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Vuralkan E.,Trabzon Kanuni Research and Training Hospital | Mutlu M.,Diskapi Yildirim Beyazit Research and Training Hospital | Firat I.H.,Diskapi Yildirim Beyazit Research and Training Hospital | Akaydin S.,Gazi University | And 4 more authors.
European Archives of Oto-Rhino-Laryngology | Year: 2014

The aim of the study was to assess the markers of oxidant-antioxidant status in patients with obstructive sleep apnea syndrome (OSAS) who underwent uvulopalatal flap (UPF) surgery. Twenty-five patients who underwent UPF surgery participated in this study. Polysomnographic examinations were performed before and after the surgery to assess sleep apnea in all patients and to determine the success of the UPF surgery regarding the improvement in the apnea-hypopnea index (AHI). Descriptive factors (BMI, age, gender and neck thickness, etc.) of patients were recorded before operation. Blood samples were taken preoperatively, and repeated postoperatively at 6-month intervals to determine the changes in serum malondialdehyde (MDA) and matrix metalloproteinase-9 (MMP-9) levels. The mean age at surgery was 45.6 ± 9.9 years (range 25-63 years). There was a significant difference between preoperative and postoperative AHI, MDA and MMP-9 values (p < 0.05). There was no significant correlation between categorical variables. There was no correlation between postoperative ODI, MMP-9 and MDA. These results indicate that OSAS is associated with abnormal lipid peroxidation, which can be improved by UPF surgery. OSAS may increase risks of cardiovascular morbidity; however, UPF might be useful for decreasing these risks in patients with OSAS who are suitable candidates for UPF surgery. © 2013 Springer-Verlag Berlin Heidelberg. Source

Akarsu S.,Izmir University | Bagirzade M.,Azerbaijan Medical University | Omeroglu S.,Gazi University | Buke B.,Kayseri Research and Training Hospital
Journal of Maternal-Fetal and Neonatal Medicine | Year: 2016

Objective: The objective of this study is to analyze the alteration in vascularization and apoptosis in the placentas of patients with Type 1 or gestational diabetes mellitus. Methods: Placental samples drawn from normal (n = 6), GDM (n = 6), and Type 1 DM (n = 6) pregnancies were rinsed in PBS and fixed in 4% paraformaldehyde. The obtained sections were examined by both light and electron microscopy. Subsequently, immunohistochemical staining was performed to evaluate apoptosis and vascularization with caspase-9 and VEGF antibodies. Results: Capillary structures in various sizes, both in free and in stem villi, were observed to be denser in the GDM group than in the control and Type-1 DM groups, utilizing electron microscopy. Similarly, when compared with Type-1 DM and controls, a decreased amount of microvilli with more irregularity and blunting on the villus surface was detected. GDM group showed increased immunoreactivity in capillaries of stem villi, free villi, and endothelial cells when compared with Type-1 DM and control groups. Regarding the immunohistochemical staining with VEGF, Type-1 DM, and GDM groups showed stronger immunoreactivity than the control group, especially in syncytiotrophoblastic cell nuclei and stromal cell nuclei. However, there was no significant difference between Type-1 DM and GDM groups. Conclusion: Type-1 DM and GDM placentas showed increased villous stromal capillarization, increased immunoreactivity with VEGF and caspase-9, and increased syncytial nodes, which may develop secondary to placental hypoxia-ischemia. However, more participants are needed to confirm these conclusions. © 2016 Informa UK Limited, trading as Taylor & Francis Group. Source

Alim A.,Cumhuriyet University | Oguzkaya-Artan M.,Erciyes University | Artan C.,Kayseri Research and Training Hospital
Nigerian Journal of Clinical Practice | Year: 2015

Aim: This study investigated the seroprevalence, complications and risk factors of Brucella infection in rural areas of Sivas, Turkey. Materials and Methods: The study was conducted in three hyperendemic counties for brucellosis known as Gurun, Altinyayla and Kangal in Sivas between April and October in 2011. A total of 1,430 subjects were consulted. Results: Of the 1,430 subjects, 217 (15.2%) with clinical findings compatible with brucellosis were examined by taking blood samples to study both standard tube agglutination test (STAT) and enzyme-linked immunosorbent assay (ELISA) (Genzyme Virotech GmbH, Rosselsheim, Germany). The seroprevalance of Brucella was found to be 8.0%. Brucella seropositivity was detected in 114 (52.5%) of the 217 subjects with STAT. There was no significant difference between female and male subjects with regard to Brucella seropositivity (P = 0.214). The seropositivity of subject 16-65 age group was significantly higher than those of subjects in <16 and >65 age groups (P = 0.001). In Brucella ELISA test results, 123 (56.7%) subjects had positive IgG antibodies and 96 (44.2%) IgM antibodies. Skeletal complications were the most frequent; joint, muscle, and waist pain were found in 87.1%, 79.7%, and 74.6% of subjects respectively. Most subjects (90.8%) gave a history of frequent consumption of fresh cheese directly from the cattle they own and contact with animals (77.8%) for risk factors of brucellosis. Conclusion: Brucella seropositivity is high in Gurun, Altinyayla and Kangal counties and primary care physicians should keep in mind the clinical and laboratory findings of brucellosis especially in family members of brucellosis patients. Source

Coban I.,Nightingale | Kokenek-Unal T.D.,Kayseri Research and Training Hospital | Alper M.,Ankara Diskapi Yildirim Beyazit Research and Training Hospital
Indian Journal of Dermatology | Year: 2015

Acroangiodermatitis is a rare self-limited angioproliferative lesion which can be associated with congenital vascular malformations or acquired venous insufficiency. Despite of its benign character, differential diagnosis of this lesion is very important because it closely resembles Kaposi sarcoma. Here we present a 26-year-old male patient with unilateral, purplish-red colored papules on his right ankle which diagnosed as acroangiodermatitis and discuss histopathological features, differential diagnosis and treatment of this unusual condition. Source

Berk E.,SB Kayseri Egitim ve Arastirma Hastanesi | Berk E.,Kayseri Research and Training Hospital | Kustimur S.,Kayseri Research and Training Hospital | Kustimur S.,Gazi University | And 2 more authors.
Mikrobiyoloji Bulteni | Year: 2011

Trichophyton rubrum is the most frequently encountered dermatophyte species causing onichomycosis. The routine diagnosis of dermatophytes depends on the direct microscopic examination (DME) and culture methods, however due to the phenotypic identification problems related to those agents, the molecular methods come into question. The aim of this study was to evaluate the diagnostic performance of real-time polymerase chain reaction (RT-PCR) for the identification of T.rubrum by comparing to DME and culture methods, from nail samples of patients with the complaints of onychomycosis. A total of 90 patients of whom 58 were male who were admitted to the dermatology outpatients clinics of our hospital with the complaints of color/shape changes in the nails and thickening of the nail, were included in the study, together with the 20 healthy volunteer subjects as controls. The nail scraping samples obtained from the patients and controls were examined with direct microscopy using 15% potassium hydroxide, dimethyl sulphoxide and chlorazole black mixture and cultivated onto Sabouraud dextrose agar with and without cycloheximide. For DNA isolation, after the disruption of nail samples with a steel tool, phenol-chloroform-isoamyl alcohol purification method were used. The amplification and demonstration of the T.rubrum DNA have been performed by using specific primers and probes following TaqMan protocol of RT-PCR (LightCycler-Roche, USA) method. Seventy-two of the patients yielded positive and 18 yielded negative results with DME. Growth of molds was detected in the cultures of 20 (27.8%) of the 72 DME positive patients and all of the isolates were identified as T.rubrum. No fungal growth was seen in the samples of 18 patients who were DME negative. In DME positive group, 67 (93%) patients were found to be positive in RT-PCR, while 8 (44.4%) patients were RT-PCR positive in DME negative group. All of the culture positive samples (n = 20) were also found positive in RT-PCR. All of the samples from the control group with healthy nails yielded negative results in DME, culture and RT-PCR methods. The performance of PCR method were compared to direct microscopy that had higher sensitivity than culture and the sensitivity, specificity, positive and negative predictive values of RTPCR assay were estimated as 93%, 56%, 89% and 67%, respectively. In conclusion RT-PCR was thought to be an efficient and rapid assay in the diagnosis of onichomycosis. Although RT-PCR seems more expensive than culture, for the centres which already have support for the molecular methods, the difference in total cost doesn't count much. In conclusion, by the use of molecular methods DNA isolation was successfully done from a relatively difficult clinical specimen, namely nail scraping, a protocole that could easily be applied in routine laboratory was established and species-level identification in a short time was accomplished in this study. Source

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