Biswas P.P.,Katihar Medical College |
Dey S.,Katihar Medical College |
Sen A.,Katihar Medical College |
Adhikari L.,Sikkim Manipal University
Journal of Global Infectious Diseases | Year: 2016
Background: The aim of this study was to find out the correlation between presence of virulence (gelatinase [gel E], enterococcal surface protein [esp], cytolysin A [cyl A], hyaluronidase [hyl], and aggregation substance [asa1]) and vancomycin-resistant genes (van A and van B) in enterococci, with their phenotypic expression. Materials and Methods: A total of 500 isolates (250 each clinical and fecal) were processed. Enterococci were isolated from various clinical samples and from fecal specimens of colonized patients. Various virulence determinants namely asa1, esp, hyl, gel E, and cyl were detected by phenotypic methods. Minimum inhibitory concentration (MIC) of vancomycin was determined by agar dilution method. Multiplex polymerase chain reaction (PCR) was used to detect the presence of virulence and van genes. Results: Out of all the samples processed, 12.0% (60/500) isolates carried van A or van B genes as confirmed by MIC test and PCR methods. Genes responsible for virulence were detected by multiplex PCR and at least one of the five was detected in all the clinical vancomycin-resistant enterococci (VRE) and vancomycin-sensitive enterococci (VSE). gel E, esp, and hyl genes were found to be significantly higher in clinical VRE. Of the fecal isolates, presence of gel E, esp, and asa1 was significantly higher in VRE as compared to VSE. The presence of hyl gene in the clinical VRE was found to be statistically significant (P = 0.043) as against the fecal VRE. Correlation between the presence of virulence genes and their expression as detected by phenotypic tests showed that while biofilm production was seen in 61.1% (22/36) of clinical VRE, the corresponding genes, i.e., asa1 and esp were detected in 30.5% (11/36) and 27.8% (10/36) of strains only. Conclusion: Enterococcus faecium isolates were found to carry esp gene, a phenomenon that has been described previously only for Enterococcus faecalis, but we were unable to correlate the presence of esp with their capacity to form biofilms. © 2016 Journal of Global Infectious Diseases | Published by Wolters Kluwer - Medknow.
Biswas P.P.,Katihar Medical College |
Dey S.,Katihar Medical College |
Adhikari L.,Sikkim Manipal University |
Sen A.,Katihar Medical College
Journal of Global Infectious Diseases | Year: 2014
Background: The aim of study was to find out the potential pathogenic role of virulence factors elaborated by strains of vancomycin resistant enterococci (VRE) isolated from clinical samples and VRE colonizing the gastrointestinal tract of hospitalized patients. Materials and Methods: Enterococci were isolated from various clinical samples and also from fecal specimens of colonized patients at the time of admission, after 48 h and after 5 days of admission. Various virulence determinants were detected by phenotypic tests. Vancomycin susceptibility in enterococci was detected by disc diffusion and agar screen method. Minimum inhibitory concentration was determined by agar dilution method. Results: Out of all the clinical and fecal samples processed, 12.0% isolates were either vancomycin resistant or vancomycin intermediate. Hemagglutinating activity against rabbit red blood cells was seen with 27.8% and 25.0% of clinical and fecal strains, respectively. Slime layer formation was seen with fecal VRE strains (37.5%) when compared to clinical VRE (27.8%). Among the clinical VRE strains the most prolific biofilm producers were Enterococcus. fecalis (92.9%) when compared to Enterococcus. faecium (52.9%). Biofilm formation/(presence of adhesions) was also seen in (29.2%) of the fecal VREs. In wound infection production of gelatinase, deoxyribonuclease (DNase), and caseinase (70.0% each) were the major virulence factors. The predominant virulence factors seen in the blood stream infection were adhesin, and hemolysin (44.4% each) and in catheter induced infection were DNase and adhesins (75.0% each). Adhesin (29.2%), slime layer (37.6%), DNAse (33.3%), gelatinase (25.0%), lipase (20.8%) and caseinase (16.6%) and hemolysin (8.3%) were produced the fecal isolates. Conclusion: An association between adhesin (as detected by biofilm formation) and urinary tract infection, adhesion and hemolysin with BSI, as also between DNase gelatinase & caseinase with wound infection was noted.
Das A.,KPC Medical College |
Kumar P.,Katihar Medical College
Dermatology Online Journal | Year: 2016
Syringomas are common appendageal neoplasms, manifested as skin-colored papules, most commonly distributed over the periorbital region. We describe a patient with syringoma localized over the neck and upper chest. © 2016 by the article author(s).
Mandal R.K.,North Bengal Medical College |
Koley S.,North Bengal Medical College |
Banerjee S.,North Bengal Medical College |
Kabiraj S.P.,North Bengal Medical College |
And 2 more authors.
Indian Journal of Dermatology, Venereology and Leprology | Year: 2013
Reed's syndrome or familial leiomyomatosis cutis et uteri is an autosomal dominant disorder, characterized by multiple cutaneous and uterine leiomyomas. We report here a case of a 53-year-old woman who presented to us with multiple painful nodules over different parts of her body. Based on the histopathological examination, imaging, and past medical records, a diagnosis of Reed's syndrome was made. Three of her sisters had similar disease. Subsequently, it was found that a total of nine members of their family in two successive generations were affected with cutaneous leiomyomas. The present case series has been reported for its interesting clinical presentations and rarity.
Ghosh A.,KPC Medical College and Hospital |
Sarkar D.,West Bengal University of Health Sciences |
Mukherji B.,Katihar Medical College |
Pal R.,All India Institute of Medical Sciences
Annals of Tropical Medicine and Public Health | Year: 2013
Background: Cardiovascular diseases will be prime cause of morbidity by 2020 in India. Objective: To determine prevalence and correlates of hypertension in an adult rural community. Materials and Methods: A cross-sectional study was carried out in the field practice area of the Department of Community Medicine of tertiary care teaching institute of Bihar among adult population to find out the prevalence of hypertension with the sociodemographic correlates from October 2009 to September 2010. A pretested predesigned questionnaire was used to collect sociodemographics data by interview technique from 997 study participants. The blood pressure was assessed and classified using recent JNC 7 and WHO criteria to grade hypertension. Results: Majority of the study participants were Muslims (89%) and in 40-49 age group (21.26%). In males and females, the overweight and obese combined were, respectively, 13%. In the dietary habit, 85.36% were non-vegetarian; 8.83% consumed salt more than 10 grams per day; 26.78% reported themselves as alcoholics; tobacco smokers were 58.28%, highest in 40-49 age group (23.58%). Overall, 11.43% were hypertensive and 16% were prehypertensive. Among hypertensive, majority were male (61%); and in 60-69 age group (27.27%), overweight and obese (56.14%), smokers (75.44%), non-vegetarian (67.54%). There was a significant linear trend between age and salt intake with prevalence of hypertension (P<0.0001). Conclusions: Hypertension in the rural population of Bihar was lower than previous estimates.
Kumar P.,Katihar Medical College |
Savant S.,Katihar Medical College |
Das A.,West Dermatology |
Hassan S.,Katihar Medical College |
Barman P.,Mahatma Gandhi Missionary New Bombay Hospital
Indian Journal of Dermatology | Year: 2015
An 18-year-old girl presented with multiple itchy hyperpigmented papules and plaques, along with tense blisters over the lower limbs and buttocks for last 3 months. These papules, plaques, and bullae were mostly localized to preexisting scars. The histopathological findings from papule and bulla were consistent with lichen planus (LP) and bullous pemphigoid, respectively. Direct immunofluorescence (DIF) of perilesional skin around bulla showed linear deposition of IgG and C3. Considering clinical, histopathological and DIF findings, diagnosis of LP pemphigoides (LPP) was made. The preferential localization of LPP lesions over preexisting scars was a very interesting finding in our case an extremely rare instance of the isotopic phenomenon. © 2015 Indian Journal of Dermatology | Published by Wolters Kluwer - Medknow.
Kumar P.,Katihar Medical College
Dermatology online journal | Year: 2014
A 5-year-old boy presented with widespread asymptomatic hyperpigmented verrucous plaques since 3 months of age. The lesions were distributed in a linear manner along Blaschko's lines on trunk and extremities and were accentuated in flexures and around joints. There was no history of blistering or redness and no other family member was affected. Ichthyosis hystrix (of Curth and Macklin) and generalized linear/mosaic epidermolytic hyperkeratosis (EHK) were considered in the differential diagnosis. Biopsy from both trunk lesion and lesion on knee revealed characteristic epidermolytic hyperkeratosis, thereby clinching the diagnosis of systematized linear EHK.
Datta S.,Katihar Medical College |
Iqbal Z.,Katihar Medical College |
Prasad K.R.,Katihar Medical College
Indian Journal of Clinical Biochemistry | Year: 2011
Acute myocardial Infarction is one of the major causes of morbidity & mortality in world and atherosclerosis is the major cause of ischemic heart disease. In order to determine the better clinical marker of atherosclerosis, we estimated serum low-density lipoprotein (LDL-C) and high sensitivity C-reactive protein (hsCRP). Hundred patients of myocardial infarction and 100 controls irrespective of age and sex were studied for these parameters over a period of 2 years. The statistical analysis showed that the serum hsCRP was significantly raised in myocardial infarction cases than controls (P < 0.01) but LDL-C was not (P > 0.05). We conclude that the serum hsCRP has better predictive value for risk of atherosclerosis. © Association of Clinical Biochemists of India 2011.
Sharma S.,Katihar Medical College |
Jha A.K.,Katihar Medical College |
Mallik S.K.,Katihar Medical College
Indian Journal of Dermatology | Year: 2011
Background: Nevus of Ota is common condition in Indian patients. The condition is more common in females, with a male-female ratio of 1:4.8. Aim : To evaluate long-term efficacy safety and stability of Q-switched ND:YAG laser in treatment of Nevus of Ota. Design: 6 month follow-up of patients of Nevus of Ota, treated with Q-switched ND:YAG laser Materials and Methods : Twenty-five patients of Nevus of Ota were treated with Q-switched ND:YAG laser for a period of 1 year and 9 months; patient had fitzpatricks skin type 4 and 5; detailed history, clinical examination, ophthalmoscopy, and otoscopy was done in all cases; clinical photographs were taken before and after the completion of treatments. Six-month follow-up was done after the last session. Response to treatment was graded based on physician′s global assessment. Result : More than 70% improvement was seen in 15 patients (60%). Eight patients (32%) had moderate and two patients (8%) showed mild improvement.
Haque N.-U.,Katihar Medical College |
Karim R.,Narayan medical college
Bangladesh Journal of Medical Science | Year: 2015
ADPKD presenting as congestive cardiac failure due to dilated cardiomyopathy is rare. ADPKD is an inherited systemic disorder with major renal manifestation and in some cases extrarenal manifestation or combination of both. In this report 45 year male patient presented with complains of dyspnoea, abdominal distension, pain right hypochondriac region. He was hospitalized, examined clinically and advised various bio-chemical and imaging tests. The finding was suggestive of ADPKD with dilated cardiomyopathy with congestive cardiac failure. He was managed with diuretics, ACE inhibitors, digoxin, Moist oxygen inhalation and he responded to the treatment. © 2015. Ibn Sina Trust. All Rights Reserved.