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Hascik P.,Katedra Hodnotenia A Spracovania Zivocisnych Produktov | Kulisek V.,Katedra Mikrobiologie | Kacaniova M.,Katedra Mikrobiologie | Pochop J.,Katedra Mikrobiologie
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2010

The aim of this study was histological and histochemical analyze of musculus pectoralis major (MPM) and musculus biceps femoris (MBF) of 12-weeks old Landes geese husbandry by sex from Hruboňovo (Czech Republic). The geese had live weight of 3979.0g and the ganders had live weight of 4779.0g. Higher a White fiber percentage representation of musculus pectoralis major and musculus biceps femoris of 12-weeks old Landes geese histological analyses we followed. Representation of sex identical MBF was 60.0% (gander) and 64.1% (geese) and MPM was 47.6% (ganders) and 51.1% (geese). The lowest α Red fibre percentage content in MPM was 6.7% (ganders) and 4.7% (geese) and β Red fiber in MBF was 10.7% (ganders) and 9.5% (geese). No statistically significant differences (P ≥ 0,05) among sex in the fat cells thickness of geese were found, but significant differences (P ≤ 0,01) was found in MBF fat cells between ganders (26.3 μm) and geese (21.9 μm). Highest thickness of a White fibre in muscles breast and femoral were found in both sex and lowest was found in β Red fibre. Muscles fibres thickness was higher femoral muscles in average (59.9 μm - ganders; 58.3 μm - geese) opposite breast muscles (47.7 μm - ganders, 44.9 μm - geese), what is the mean higher consistence of femoral muscles for consumer. In term of lowest musculus fiber thickness of Landes geese in average were 44.9 μm - MPM, 58.3 μm - MBF opposite of ganders 47.7 μm - MPM, 59.9 μm - MBF. Higher a White fibre representation was both muscles (51.1% - MPM, 64.1% - MBF). We recommended for experience used individual rearing of male. Source


Hascik P.,Katedra Hodnotenia A Spracovania Zivocisnych Produktov | Kacaniova M.,Katedra Mikrobiologie | Bobko M.,Katedra Hodnotenia A Spracovania Zivocisnych Produktov | Pochop J.,Katedra Mikrobiologie | And 2 more authors.
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2011

In the experiment were verified the application of probiotic preparation through a water supply for feeding of cock's hybrids Ross 308, Hubbard JV and Cobb 500 in the chemical composition of the most valuable parts of the carcass. Probiotic was based on the strain Lactobacillus fermentům with containing of 1.109 cfu.g-1 and potentially components of maltodextrin and oligofructose in 1% concentration. Length of feeding period was 42 days. Cocks were fed an ad libitum with the same starter mixture HYD-01 to 21-th- day and from 22-nd to 42-nd day of feeding with mixture HYD-02 in powdery form. The average of protein content of breast muscle was highest in Hubbard JV hybrid (23.93 g.100 g-1), lower in Cobb 500 hybrid (23.90 g.100 g-1) and lowest in Ross 308 hybrid (23.73 g.100 g-1), without significant differences (P ≥ 0.05) between hybrids and hybrids groups. Effect of probiotics had increased the protein content (P ≥ 0.05) in breast muscle of Ross 308 and Cobb 500 cocks and at the Hubbard JV only lower doses application during the feeding. The average of fat content in lOOg of breast muscle was lowest in Cobb 500 hybrid (1.09 g), higher in Hubbard JV hybrid (1.28g) and highest in Ross 308 hybrid (1.35 g). Effect of probiotic to reduce fat content in breast muscle of cocks was at Ross 308 hybrid (1.33 and 1.23 g.100 g-1), Cobb 500 hybrid (0.98 and 1.02 g.100 g-1) and in second experimental group at Hubbard JV hybrid (1.03 g.100 g-1) statistically significant (P > 0.05) in compared with control group, but significantly (P ≤0.05) between hybrids Cobb 500 and Hubbard JV in the first test groups. The average of energy value in lOOg of breast muscle was highest in Hubbard JV hybrid (449.24 kj), lower in Ross 308 hybrid (448.40 kj) and lowest in Cobb 500 hybrid (441.45 kj), without significant differences (P ≥ 0.05) between hybrids and hybrids groups. The average of protein content of the femur was highest in Ross 308 hybrid (18.56 g.100 g-1), lower in Cobb 500 hybrid (18.42 g.100 g-1) ) and lowest in Hubbard JV (17.54 g-1) without statistical significance (P ≥ 0.05). Significant differences (P ≤ 0.05) of the femur were found at lower dose of probiotics between cock's hybrids Hubbard JV (16.95 g.100 gr1) and Ross 308 (18.48 g.100 g-1) in the protein content. The average of fat content in the femur was highest in Ross 308 hybrid (10.58 g.100 g-1), lower in Hubbard JV hybrid (10.51 g.100 g-1) and lowest in Cobb 500 hybrid (10.29g.100 g-1) without significant differences (P ≥ 0.05) between hybrids and hybrids groups. The higher fat and protein content in 100 g of the femur in Ross 308 hybrid was ensured the highest energy value (710.88 kj), lower in Cobb 500 hybrid (696.40 kj) and lowest in Hubbard JV hybrid (689.77 kj) without significant differences (P ≥0.05) between hybrids and hybrids groups. The verified probiotic preparation in the final analysis had not negative effect on the chemical composition of the most valuable parts of the carcase cock's hybrids Ross 308, Hubbard JV and Cobb 500. Source


Lahucky R.,Katedra Hodnotenia A Spracovania Zivocisnych Produktov | Bucko O.,Katedra Hodnotenia A Spracovania Zivocisnych Produktov | Hascik P.,Katedra Hodnotenia A Spracovania Zivocisnych Produktov | Liptaj T.,Slovak University of Technology in Bratislava
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2012

The effects of supplementing the diet of pigs with creatine monohydrate (CMH) and vitamin E on blood plasma creatine concentration, vitamin E level in muscle, muscle energy metabolism, antioxidant capacity and meat (musculus longissimus dorsi) quality of pigs (DNA tested and negative on malignant hyperthermia) were investigated. Two treatments were used: supplementation with CMH alone (2 g.kg-1 of feed, 10 days before slaughter); and supplementation with both vitamin E (500 mg α-tocopherol.kg-1 of feed for minimum of 30 days) and CMH (2 g.kg-1 of feed, 10 days before slaughter). Pigs supplemented with CMH alone showed elevated plasma creatine concentration (P ≤ 0.05). Phosphorus nuclear magnetic resonance (31P NMR) measurements on post mortem (15 min.) muscle samples showed the highest phosphocreatine levels and ratio PCr/Pi (P ≤ 0.05) in CMH supplemented pigs alone and in combination with vitamin E. Dietary supplementation with vitamin E significantly (P ≤0.05) increased the concentration of α-tocopherol in meat. Supplementation with CMH alone or in combination with vitamin E resulted in higher (P = 0.07) a values of loin chops at 5 days of storage. Antioxidative capacity (measured as MDA production after incubation of longissimus muscle homogenates with Fe2+/ ascorbate) was substantially improved by vitamin E and somewhat by CMH supplementation. Source

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