Racek M.,Katedra Biotechniky Parkovych A Krajinnych Uprav |
Lichtnerova H.,Katedra Biotechniky Parkovych A Krajinnych Uprav |
Dragunova M.,Katedra Biotechniky Parkovych A Krajinnych Uprav |
Gajdosova A.,Katedra Biotechniky Parkovych A Krajinnych Uprav |
Jakabova A.,Ustav Genetiky A Biotechnologii SAV
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2010
Experimental works were focused on creation of optimal protocol for Acer davidil ssp. grosseri shoots multiplication. As the basic medium we used MS (Murashige a Skoog) culture medium enriched by cytokinins TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) in different concentrations. There were used single nodal segments from four years old mother plants in experiments. Experiments were established in the middle of June in the phase of rapid growth of shoots. Cultivation conditions were: 21°C, light intensity 40 μmol.m -2.s-1, period of light 16 hours during the day. The reactions of expiants were evaluated after six and twelve month. The results were statistically analysed. The explants of Acerdavidii ssp. grosseri reacted differently on hormonal regulation. The lowest multiplication activity of expiants was found out on MS culture medium enriched by 2,6 μM BAP. The average shoot multiplication on MS + 0,5 μM TDZ culture medium was 1,2. Multiplication 1,49 shoot per expiants was found out on MS + 2,6 μM BAP + 0,5 μM TDZ. On the basic MS medium 1,3 5 shoots per expiants multiplicated. In spite of positive influence of application of TDZ and BAP on shoot multiplication the results compared with multiplication on MS medium were statistically insignificant.
Kerenyi-Nagy V.,University of West Hungary |
Bakay L.,Katedra biotechniky parkovych a krajinnych uprav |
Bohm E.I.,University of West Hungary
Journal of Landscape Ecology | Year: 2013
In this article we published some new Rosa, Crataegus and Pyrus datas from Cebovce: Ko-hegy (Central Slovakia). The most important findings were Crataegus ovalis Kit. (7th locality in Slovakia, cf. Baranec, 1986), Rosa bohemica H. Braun (1st locality in the county) and Pyrus × amphigenea Domin ex Dostalek. The research was supported by "VEGA 1/0246/13 Stratégie využitia vody xerofytnými drevinami a bylinami v urbanizovanom prostredí a krajine" project.