Karpagam Arts and Science College

Coimbatore, India

Karpagam Arts and Science College

Coimbatore, India
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Praveesh B.V.,Karpagam Arts and Science College | Angayarkanni J.,Bharathiar University | Palaniswamy M.,Karpagam University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

Milk proteins are precursors of many different biologically active peptides. These peptides are inactive within the sequence of the precursor proteins but can be released during microbial fermentation. In the present study, the cow milk hydrolysate showed multifunctional properties like angiotensin converting enzyme inhibition, antioxidant and anticancer activity. The optimized fermentation conditions for angiotensin converting enzyme inhibitor production by the combination of Lactobacillus plantarum and Lactobacillus casei were also studied. Angiotensin converting enzyme inhibition activity (69.28±0.37%) to be highest at 370°C with inoculum concentration of 1% after 24 hours of incubation. The milk hydrolysate exhibited scavenging potential with IC 50 value of 185.87 μg/ml for 2, 2-diphenyl-1- icrylhydrazyl. And also the cow milk hydrolysate showed in vitro anticancer activity against HeLa cell with IC 50 of 150 μg/ml.

Jeyaramraja P.R.,Hamelmalo Agricultural College | Thushara S.S.,Karpagam Arts and Science College
Photosynthetica | Year: 2013

Responses of drought-tolerant (DT) and drought-susceptible (DS) pot-grown groundnut (Arachis hypogaea L.) varieties to changes in leaf relative water content (RWC) were studied. Water stress (WS) was imposed on 30-day-old plants for 2 weeks. Leaf RWC decreased significantly under WS conditions with simultaneous decrease in net photosynthetic rate (P N) and stomatal conductance (g s). Even though no significant difference was observed between DT and DS varieties with regard to RWC, DT varieties were able to maintain significantly higher P N than DS varieties. Higher values of water use efficiency (WUE) were also observed in DT varieties during WS conditions. The decline in P N due to WS could be attributed to both reduction in g s (i.e. stomatal limitation) and to reduction in chlorophyll content (Chl). No significant difference in leaf area index (LAI) was found between DT and DS types and LAI was not reduced by WS. Significant differences were found among the studied groundnut varieties, but not between DT and DS types, in terms of root, aboveground, and total dry mass. These growth parameters significantly decreased under WS conditions. Based on the results, a sequence of physiological responses in groundnut crop subjected to WS was postulated. © 2013 Springer Science+Business Media Dordrecht.

Vijayakumar R.,Karpagam Arts and Science College | Jagannathan S.,Pasteur Institute of India | Chaansha S.,Kongunadu Arts and Science College
Nano Biomedicine and Engineering | Year: 2011

The present era of nanotechnology has reached to a stage where scientist are able to develop and programme complex machines that are built at molecular level which can work inside the patient body. One such challenge is a nanorobot, which once thought to be a desire as came into reality now. The proposed application of nanorobot can range from common cold to dreadful diseases like cancer, Diabetes, influenza, cerebral aneurysm. This study of nanorobot serves as a lead to the field of nanomedicine. There are many applications for nanorobotic systems and its biggest impact would be in the area of medicine. This article deals with the nanorobotic design and their applications in molecular diagnosis. © 2011 Vijayakumar R, et al.

Vishnupriya B.,Karpagam Arts and Science College | Selvam K.,NGP
International Journal of ChemTech Research | Year: 2010

Lipases are enzymes, which are widely used in many industries. The actinomycetes member streptomyces griseus strain was purchase from MTCC 4734(Microbial type culture collection-Chandigarh), which was having lipase coding gene. In the present study, Several factors affecting the activity of lipase were investigated. Olive oil was the best substrate for enhancing the enzyme activity. Due to high cost, instead of using olive oil, sunflower oil and palm oil were used as a substrate for lipase production and the enzyme activity was determined by titrimetic method. In this study, olive oil was using as a standard (standard), while sunflower oil and palm oil as a (test). The maximum lipase activity was achieved at 24 & 48 h of incubation period and the enzyme activity was 51.9 and 51.9U/ml by using sunflower oil and palm oil as a substrate. The incubation period was very short for obtaining maximum lipase by using sunflower oil than palm oil. No significant changes obtained in the pH ranges from 6 to 9.

Rani S.A.,Karpagam University | Sundaram L.,Karpagam University | Vasantha P.B.,Karpagam Arts and Science College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

L-asparaginase has been used as anti-tumor agent for the effective treatment of acute lymphoblastic leukemia and as food processing aid to reduce the acrylamide formation during frying of starchy foods at high temperature. In the present study thirty eight fungal strains were isolated from soils which collected from different parts of India. The isolated fungi were screened for L-asparaginase production using Czapek's agar media. On the basis of pink colour zone formed, one fungal strain was selected and identified as Aspergillus sp (KUFS20). A comparative study was carried out to evaluate L-asparaginase production in submerged and solid state fermentation using different substrates. Among the twenty substrates used, orange peel (70.67±1.14 U/g) showed maximum enzyme production.

Rani S.A.,Karpagam University | Sundaram L.,Karpagam University | Vasantha P.B.,Karpagam Arts and Science College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

The in vitro antioxidant and antitumor activity of partially purified L-asparaginase enzyme from Aspergillus flavus (KUFS20) was studied. The enzyme showed a good scavenging activity against DPPH with IC 50 value of 263.63 μg/ml. It also showed total reducing activity. To determine anticancer activity, different concentration of partially purified L-asparaginase was tested on MCF-7 cancer cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The enzyme showed a significant antiproliferative activity and a dose dependent effect was observed. Minimum inhibition of 4.78% was shown by enzyme at concentration 1.953 μg/ml and maximum inhibition (67.85%) was observed at 1000 μg/ml. From the result it concludes that this L-asparaginase can be used for the development of new preparations for the therapy of tumour.

Damodharan U.,Karpagam Arts and Science College | Ganesan R.,Karpagam University | Radhakrishnan U.C.,Karpagam University
Applied Biochemistry and Biotechnology | Year: 2011

Matrix metalloproteinases (MMPs) are a family of zinc-dependent neutral endopeptidases, collectively capable of degrading essentially all matrix components. Elevated levels of distinct MMPs are detected in tumor tissue or serum of patients with advanced cancer, and they are the major prognostic indicators in cancer. Inhibition of MMPs has been explored as a therapeutic goal for almost two decades. Nitric oxide (NO), a free radical plays an important role in signaling pathways in regulation of MMP expression. In the present study, we demonstrated the role of exogenous NO levels in the regulation of MMP2 and MMP9 (gelatinases A and B) in colon cancer cell line WiDr and its inhibition with emodin (a naturally occurring anthraquinone). © 2011 Springer Science+Business Media, LLC.

Jagannathan S.,Pasteur Institute of India | Gandhi P.R.,Jamal Mohammed College | Vijayakumar R.,Karpagam Arts and Science College
Journal of Biological Sciences | Year: 2013

Rabies is an acute disease of vertebrate animals which may affect all animals from amphibian to man and it is a single stranded negative sense RNA virus belonging to the genus Lyssavirus of the family Rhabdoviridae. The production of vaccines, diagnosis and further research will therefore continue in this field because a cure for rabies would be a big boon for the developing countries. Virus inactivation is essential for the preparation of vaccines, diagnostic reagent and research purposes. The beta propiolactone (βPL) is widely used as an inactivatent; βPL is very considerable value in the production of biological for virus inactivation and perhaps for DNA inactivation. The inactivation results indicate that the rate of inactivation is directly proportional to the concentration of βPL used. Assessment of the residual βPL present in the vaccine is a mandatory test to vaccine. Vaccine manufacturing also assess the efficacy of inactivation procedure by various in process quality control tests such as tissue culture virus amplification test and inactivation kinetics. This experiments are based on the changing the parameters as well as the various concentration of βPL in the clarified, concentrated high titer of P VI1 rabies strain, subsequently that are further purified and formulated with suitable additives and stabilizers. The immunogenicity of the vaccines is analyzed. During the βPL inactivation kinetics gives an idea of the predictable time of inactivation and also the order of inactivation, validation of the inactivation process is an essential part of quality assurance. In the immunobiological industry the time is a crucial factor because to get more inactivated viral harvest yield in a short period and they are processed to further purification and downstream processing. This findings is to reduce the time frame and the residual βPL in various level influenced the tissue culture derived anti rabies vaccine. © 2013 Asian Network for Scientific Information.

Begum S.F.M.,Sri Krishna Arts and Science College | Anuradha R.,Karpagam Arts and Science College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

Plants and their extracts have been the basis of treatment of human diseases since time immemorial. Use of plants as a source of immunomodulators is under extensive use and significances. In the present study, the aqueous extract of Asparagus racemosus significantly enhanced the total WBC count, hemoglobin content and bone marrow cellularity in animals treated with Cyclophosphamide. It also enhanced the weight of lymphoid organs such as thymus and spleen, which indicates its effect on cellular immune response. The number of α-esterase positive cells, which were decreased by CTX administration, was also found to be increased by Asparagus racemosus treatment indicating its effect on stem cell differentiation. These observation indicated that the Asparagus racemosus could overcome the immunosuppression induced by Cyclophosphamide. Thus it can be concluded that the Asparagus racemosus can be used as an adjuvant along with cancer chemotherapy.

Vadivel V.,Karpagam Arts and Science College
Indian Journal of Traditional Knowledge | Year: 2010

The effect of various common processing methods on the nutritional value, antinutritional compounds, biological value and protein quality of dehulled seeds of an under-utilized tribal food legume, Tamarindus indica L. was investigated. The Dehulled Tamarind Seeds (DTS) were found to contain 23.19% of protein, 8.8% of lipid, 7.2% of fiber, 4.6% of ash and 56.1% of carbohydrates. Although, various antinutritional compounds were present in the DTS, the autoclaving treatment was found to effectively reduce their maximum levels without affecting the nutritional value of DTS. When considering the biological value, the rats fed with diet containing autoclaved DTS exhibited better growth performance. Moreover, the protein quality of DTS such as True Digestibility, Biological Value, Net Protein Utilization and Utilizable Proteins were also significantly improved by autoclaving treatment when compared to other processing methods such as soaking, cooking and roasting.

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