Karnataka Veterinary, Animal and Fisheries Sciences University

Bidar, India

Karnataka Veterinary, Animal and Fisheries science University is a public university. It was established by an Act of Karnataka Legislative Assembly which was passed on 10 February 2004. The university was formerly established and inaugurated on 28 February 2005. This university has its headquarters in the Bidar district of Karnataka state of India.As the name says this university was created for the education and control of veterinary, animal and fisheries science education in the state of Karnataka. As many as 21 allied institutions including colleges, research institutions, veterinary hospitals among others and the employees are transferred to the new university. These include Veterinary College, Bangalore; College of Veterinary Science & Animal Husbandry and Veterinary College, Bidar; Veterinary College Hospital, Hebbal; Dairy Science College, Bangalore; Rural Veterinary Hospital at Yelahanka; District Veterinary Hospital, Bidar of Animal Husbandry and Veterinary Services Department.Institutions relating to fisheries which are transferred under the new university include College of Fisheries at Mangalore; Fisheries Research Station, Hesaraghatta; Fisheries Research Station, Ankola; Fish Seed Production farms at Munirabad and Shivapura, in Koppal district.Other institutions under the university are the Institute of Animal Health and Veterinary Biologicals, Bangalore; livestock farm at Hesaraghatta; Livestock Breeding and Training Centre, Kurikuppe, Bellary; Devani Cattle Breeding Station, Hallikhed, Bidar; Killar Breeding Station, Arabhavi; Sheep Breeding, Bandoor, Malavalli; Main Research Station, Hebbal; Agriculture Research Station at Mangalore and Nagamangala and Zonal ARS at Konnehally, Tiptur. Wikipedia.

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Basavaraja N.,Karnataka Veterinary, Animal and Fisheries Sciences University | Raghavendra C.H.,Indian Central Institute of Freshwater Aquaculture
Aquaculture International | Year: 2017

The present investigation was conducted with a view to produce a female-free population and to test the heredity of body colour in tilapia. In this study, 6-day-old red tilapia were fed diets incorporated with 0, 25, 50, 75 and 100 mg/kg diet of a synthetic androgen, 17α-methyltestosterone (17α-MT), for 30 days. Administration was followed by 120 days of rearing in nylon hapas where hormone-free diet was given. At the end of the 120-day rearing, 17α-MT at 50 ppm induced 100% sex reversal, whereas 25, 75 and 100 mg/kg resulted in incomplete sex reversal. Immersion of newly hatched red tilapia fry in an aqueous solution of 17α-MT at 200 μg/L for 1 h produced 73% males; however, when given to 2-day-old fry, it yielded 78% males. The results indicate that oral administration of 17α-MT is more effective than treatment by immersion to produce male dominant red tilapia populations. Treatment of red tilapia with the androgen did not indicate any definite trend in the gonado-somatic index of both males and females. The results of progeny testing indicate that red tilapia does not belong to either XX-XY or WZ-ZZ sex determination systems, the two most common sex determination systems that exist in fish. When mating, Oreochromis mossambica (black tilapia) and red hybrid tilapia produced F1 progeny with a brown colour, the intermediate colour between the two parental body colours. When F1 hybrids were interbred, the three phenotypes got separated in the F2 generation with a 1 black/2 brown/1 red phenotypic ratio as expected of a single autosomal gene with incomplete dominant gene action. The importance of monosex male red tilapia farming and body colour inheritance is also discussed. © 2017 Springer International Publishing Switzerland

Gowda A.K.J.,Karnataka Veterinary, Animal and Fisheries Sciences University
Journal of Parasitic Diseases | Year: 2014

The present work was carried out to study the sero-prevalence of Haemonchus contortus infection in sheep by Indirect-Enzyme Linked Immuno Sorbent Assay (Indirect-ELISA) using somatic antigen. Out of 100 abomasums screened, 57 found positive for H. contortus adult worms. A total of 250 serum samples which includes, 100 serum samples from local abattoir in and around shimoga region from the animals from which the abomasums were collected and 150 serum samples from migratory sheep were used to detect the circulating antibody against H. contortus by Indirect-ELISA using somatic antigen. Of the 57 sheep harboring adult worms in their abomasums, the serum samples showed positive reaction by Indirect-ELISA with somatic antigen. However, among 43 sheep which are not showing any adult worms of H. contortus in their abomasums, but their 21 serum samples showed positive reaction by Indirect-ELISA. The sensitivity and specificity of Indirect-ELISA was found to be 100 and 67.18 %, respectively. Also, the sero-prevalence of H. contortus infection was found to be 58.66 % out of 150 migratory sheep serum samples screened for detecting circulating antibodies of H. contortus by Indirect-ELISA using somatic antigen in and around shimoga region. © 2014 Indian Society for Parasitology.

Maiti B.,Karnataka Veterinary, Animal and Fisheries Sciences University | Shetty M.,Karnataka Veterinary, Animal and Fisheries Sciences University | Shekar M.,Karnataka Veterinary, Animal and Fisheries Sciences University | Karunasagar I.,Karnataka Veterinary, Animal and Fisheries Sciences University
Veterinary Immunology and Immunopathology | Year: 2012

Aeromonas hydrophila is an important fish pathogen responsible for huge economic losses in aquaculture sector. The bacterial outer membrane proteins (OMPs), especially adhesins play a key role in the virulence of the bacteria and are considered potential vaccine candidates. We evaluated the immunogenicity of two important outer membrane proteins namely Aha1 and OmpW of A. hydrophila. These proteins were over-expressed in Escherichia coli, purified and used for the vaccination of common carp. Sequence analysis predicted that, Aha1 and OmpW are adhesins and antigenic. Common carp immunized with recombinant Aha1 and OmpW proteins showed significant antibody production and a relative percentage survival of 52 and 71 respectively indicating their protective efficacy against A. hydrophila infection. © 2012 Elsevier B.V.

Javaregowda A.K.,Karnataka Veterinary, Animal and Fisheries Sciences University
Journal of Parasitic Diseases | Year: 2016

The present study was conducted to observe the prevalence of endo-parasites in wild carnivores maintained under captive state at Tyavarekoppa Tiger and Lion Safari unit near to Veterinary College, Shimoga. A total of 54 wild carnivores were included in the study and the fresh fecal samples were collected, examined on the same day by direct and sedimentation techniques for endo-parasitic eggs/ova in the laboratory. Out of 15 tigers samples screened, 12 were harboring mixed infections of Strongyle spp., Toxocara spp, oocysts of coccidia and Spirometra spp. ova/eggs. Among 21 leopards sample screened, only 11 fecal samples showed eggs of Toxocara spp. and three showed eggs/ova of Spirometra spp. Of the 12 lion fecal samples examined, only 3 harbored eggs Toxocara spp. and two showed mixed infections of Strongyle spp., Toxocara spp, oocysts of coccidia. Among six Jackals screened, three faecal samples found positive for Strongyle spp. and Toxocara spp. eggs/ova. © 2015, Indian Society for Parasitology.

Agency: European Commission | Branch: FP7 | Program: CP-FP-SICA | Phase: KBBE.2011.2.5-02 | Award Amount: 3.97M | Year: 2012

Food security is a major concern for all countries in the face of population increase and diminishing energy and water supplies. Over one billion people in low and middle income countries suffer from malnutrition. To meet the UN Millennium Development Goals to eradicate hunger and poverty, it is essential to reduce post harvest losses, including in the fisheries sector. The overall objectives of SECUREFISH are to strengthen capacity in low cost technology; to improve the preservation of existing fish supplies; to utilise waste and bycatch to produce value-added products; to develop an integrated quality management tool and finally to test the developed technology and quality management tool in different real third country conditions. There are six work packages (WP). WP1 will ensure the efficient management of the project. WP2 will develop low cost innovative processing tools based on traditional technology for preserving fish including a solar tunnel drier, a modified solar assisted extruder and fast freezing/ continuous atmosphere freeze-drier (CAFD). In WP3, underutilised bycatch and waste by-products of fish processing will be recovered and converted to high value products. WP4 will develop an effective total quality management tool (safety and risk assessment; HACCP quality cost and traceability, nutritional and eating quality and carbon footprint) of three fish product chains (solar dried, extruded and frozen/CAFD) which will be tailored to suit local needs. The technological advances (WP2) and quality management tool (WP4) will be evaluated in the three fish product chain case studies in Africa (Kenya, Namibia, Ghana), Asia (India and Malaysia) and Latin America (Argentina) to include different economic, cultural and social conditions. The case studies involve stakeholders including SMEs to ensure sustained implementation of project results. WP6 details a strategy for education, training and dissemination to widely promote the results and guidelines.

Ruwandeepika H.A.D.,Ghent University | Ruwandeepika H.A.D.,Karnataka Veterinary, Animal and Fisheries Sciences University | Defoirdt T.,Ghent University | Bhowmick P.P.,Karnataka Veterinary, Animal and Fisheries Sciences University | And 2 more authors.
Environmental Microbiology | Year: 2011

Vibrios belonging to the Harveyi clade are pathogenic marine bacteria affecting both vertebrates and invertebrates, thereby causing a severe threat to the aquaculture industry. In this study, the expression of haemolysin, metalloprotease, serine protease, the quorum sensing master regulator LuxR and the virulence regulator ToxR in different Harveyi clade isolates was measured with reverse transcriptase real-time PCR with specific primers. There was relatively low variation in the in vitro expression levels of the quorum sensing master regulator luxR (sevenfold), whereas for the other genes, the difference in expression between the isolates showing lowest and highest expression levels was over 25-fold. Furthermore, there was a significant correlation between expression levels of toxR and luxR and between the expression levels of these regulators and the protease genes. The expression levels of luxR, toxR and haemolysin were negatively correlated with the survival of brine shrimp larvae challenged with the isolates. Finally, a non-virulent, a moderately virulent and a strongly virulent isolate were selected to study in vivo expression of the virulence genes during infection of gnotobiotic brine shrimp larvae. The in vivo gene expression study showed a clear difference in virulence gene expression between both virulent isolates and the non-virulent isolate. © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.

Rai P.,Karnataka Veterinary, Animal and Fisheries Sciences University | Safeena M.P.,Karnataka Veterinary, Animal and Fisheries Sciences University | Karunasagar I.,Karnataka Veterinary, Animal and Fisheries Sciences University
Virus Research | Year: 2011

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) of shrimp, recently been classified as Penaeus stylirostris densovirus (PstDNV). The complete nucleic acid sequence of PstDNV from India was obtained by cloning and sequencing of different DNA fragment of the virus. The genome organisation of PstDNV revealed that there were three major coding domains: a left ORF (NS1) of 2001. bp, a mid ORF (NS2) of 1092. bp and a right ORF (VP) of 990. bp. The complete genome and amino acid sequences of three proteins viz., NS1, NS2 and VP were compared with the genomes of the virus reported from Hawaii, China and Mexico and with partial sequence available from isolates from different regions. The phylogenetic analysis of shrimp, insect and vertebrate parvovirus sequences showed that the Indian PstDNV isolate is phylogenetically more closely related to one of the three isolates from Taiwan (AY355307), and two isolates (AY362547 and AY102034) from Thailand. © 2011 Elsevier B.V.

Binsi P.K.,Karnataka Veterinary, Animal and Fisheries Sciences University | Shamasundar B.A.,Karnataka Veterinary, Animal and Fisheries Sciences University
Food Chemistry | Year: 2012

The transglutaminase (TGase) enzyme from four different fish species, namely bigeye snapper, Indian oil sardine, tilapia and common carp have been isolated and characterised. The specific activity of the enzyme was higher in tilapia followed by oil sardine, common carp and bigeye snapper. The molecular weight of pure TGase was found to be in the range of 73-95 kD for four different fish species. The temperature optima for maximum activity of TGase varied amongst four species studied. The effect of activator (calcium chloride) and inhibitors (ethylenediaminetetraacetic acid (EDTA), ammonium chloride and free lysine-hydrochloride) at different concentrations on the TGase enzyme activity have been evaluated. The addition of isolated TGase to fish mince from Cynoglossus sp. was attempted in order to evaluate the setting and gelling ability. The setting and gelling ability of fish mince in presence of TGase improved considerably as revealed by small strain and large strain test. © 2011 Elsevier Ltd. All rights reserved.

NaveenKumar S.,Karnataka Veterinary, Animal and Fisheries Sciences University | Shekar M.,Karnataka Veterinary, Animal and Fisheries Sciences University | Karunasagar I.,Karnataka Veterinary, Animal and Fisheries Sciences University
Virus Research | Year: 2013

Macrobrachium rosenbergii nodavirus (MrNV) is responsible for the newly emerging catastrophic disease known as white tail disease (WTD) in M. rosenbergii. The complete sequence of RNA2 (1175. bp) and 3126. bp region of RNA1 of an Indian strain of MrNV was generated. Sequence analysis of RNA2 revealed the presence of a single ORF encoding a capsid protein of 371 amino acids with a predicted molecular mass and p. I of 41.5. kDa and 8.97 respectively. RNA1 contained two ORFs, one encoding a partial RNA dependent RNA polymerase (RdRp) of length 1034 amino acids and another a B2-like protein with a length 133 amino acids. A phylogenetic analysis based on the amino acid sequence of the capsid protein, to related nodavirus sequences suggests the establishment of new genotypes within the Nodaviridae family and we suggest the name should be genus Gammanodavirus. A new reverse transcriptase-polymerase chain reaction (RT-PCR) assay has been developed and optimized for the detection of shrimp nodavirus with a sensitivity to detect up to 24 copy numbers of plasmid construct. © 2013 Elsevier B.V.

Saravanan V.,Gothenburg University | Saravanan V.,Karnataka Veterinary, Animal and Fisheries Sciences University | Godhe A.,Gothenburg University
European Journal of Phycology | Year: 2010

Water samples and plankton net hauls were collected 24 times from Gullmar Fjord on the Swedish west coast from February 2004 to March 2005. The abundance of Skeletonema marinoi was estimated and individual clones isolated. Abundance was highest during the spring blooms in February to March. Subsequently, S. marinoi was detected in all samples but at lower abundances. At the end of September a second peak was recorded. All clones were pre-adapted to the same culturing conditions for more than 1.5 years. Large subunit (LSU) rDNA (D1-D3) was sequenced from 23 clones isolated from three different seasons, February, June and September. Six microsatellite loci were genotyped for 19 clones to estimate within-season genetic diversity. Three clones from each season were selected for physiological experiments at different salinity and temperature combinations and monitored for average number of divisions per day, maximum cell densities, biovolume, and total RNA concentration per cell. Differentiation of physiological response among the clones was partly attributed to the month of isolation. The February isolates had a significantly higher division rate and larger biovolumes, while the September clones attained higher cell densities. The June clones were isolated during the time of the year when the natural abundance is lowest, and exhibited the smallest genetic and physiological variation, which suggests that they were under strong selection pressure. The differential physiological responses and degree of genetic heterogeneity among seasonally separated clones could indicate that different populations succeed each other in the fjord. © 2010 British Phycological Society.

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