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Wadhawa G.C.,Karmaveer Bhaurao Patil College | Shivankar V.S.,Karmaveer Bhaurao Patil College | Patil S.S.,Karmaveer Bhaurao Patil College | Gaikwad Y.A.,Karmaveer Bhaurao Patil College | And 4 more authors.
Rasayan Journal of Chemistry | Year: 2017

Alum, have been investigated for their catalytic activity in the condensation reaction between o-phenylenediamine and an aldehyde or a ketone to synthesizes 2-substituted benzimidazole and 1,5-disubstituted benzodiazepines respectively. The isolated yields of 2-substituted benzimidazole and 1,5-disubstituted benzodiazepines are in the range of 30% to 95%. This method has been found to be simple and economical. The solid supports could be regenerated and reused without much loss in their activity. Further, the solid supports have been also found to be effective as general catalysts in the condensation of o-phenylenediamine with other substituted aldehydes and ketones. © RASĀYAN. All rights reserved.


Patil R.J.,Karmaveer Bhaurao Patil College
Rasayan Journal of Chemistry | Year: 2012

Various high molecular weight amines (liquid anion exchangers) are used for solvent extraction studies of metals. Extractive separation of Bi(III) from thiocyanate media using 4-Methyl-N-n-octyl aniline have been reported in this paper. Bi(III) was quantitatively extracted from 0.5M KSCN and 1.0M sulphuric acid with equal volume of 2% 4- Methyl-N-n-octyl aniline in xylene. It was stripped from the organic phase with acetate buffer and estimated complexometrically. The effects of acidity, thiocyanate concentration, reagent concentration, diluents, foreign ions and aqueous to organic phase volume ratio on the extraction have been discussed. Nature of extracted species and extraction mechanism is discussed. The method is applied to synthetic mixtures & alloy. It is fast, accurate & precise. © 2012 RASĀYAN. All rights reserved.


Yadav S.P.,Karmaveer Bhaurao Patil College | Shinde S.S.,Shivaji University | Kadam A.A.,College of Engineering, Pune | Rajpure K.Y.,Shivaji University
Journal of Alloys and Compounds | Year: 2013

The manganese (Mn) substituted cobalt ferrite (Co1-xMn xFe2O4) is synthesized by simple ceramic method with x varied from 0 to 0.5 in step of 0.1. The role of Mn substitution on structural, morphological, dielectrical, magnetic and impedance properties of cobalt ferrite has been investigated. Phase identification was performed by X-ray diffraction analysis which shows the spinel cubic crystal structure. The lattice parameter is found to increase with increase in Mn content. The saturation magnetization (Ms) and coercivity (Hc) are measured from hysteresis plots. Dielectric dispersion having Maxwell-Wagner-type interfacial polarization has been observed for cobalt ferrite samples. Dielectric constant and loss tangent are found to decrease with frequency. AC conductivity measurements suggest that the conduction is due to small polaron hopping. The semiconducting behavior of the samples has been studied by dc resistivity measurements. Room temperature complex impedance analysis shows semicircle attributed to the high resistance values at lower frequencies. © 2012 Elsevier B.V. All rights reserved.


Ghosh S.B.,Bhabha Atomic Research Center | Bhattacharya K.,Bhabha Atomic Research Center | Nayak S.,Karmaveer Bhaurao Patil College | Mukherjee P.,Bhabha Atomic Research Center | And 2 more authors.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

Definitive identification of microorganisms, including pathogenic and non-pathogenic bacteria, is extremely important for a wide variety of applications including food safety, environmental studies, bio-terrorism threats, microbial forensics, criminal investigations and above all disease diagnosis. Although extremely powerful techniques such as those based on PCR and microarrays exist, they require sophisticated laboratory facilities along with elaborate sample preparation by trained researchers. Among different spectroscopic techniques, FTIR was used in the 1980s and 90s for bacterial identification. In the present study five species of Bacillus were isolated from the aerobic predigester chamber of Nisargruna Biogas Plant (NBP) and were identified to the species level by biochemical and molecular biological (16S ribosomal DNA sequence) methods. Those organisms were further checked by solid state spectroscopic absorbance measurements using a wide range of electromagnetic radiation (wavelength 200 nm to 25,000 nm) encompassing UV, visible, near Infrared and Infrared regions. UV-Vis and NIR spectroscopy was performed on dried bacterial cell suspension on silicon wafer in specular mode while FTIR was performed on KBr pellets containing the bacterial cells. Consistent and reproducible species specific spectra were obtained and sensitivity up to a level of 1000 cells was observed in FTIR with a DTGS detector. This clearly shows the potential of solid state spectroscopic techniques for simple, easy to implement, reliable and sensitive detection of bacteria from environmental samples. © 2015 Elsevier B.V. All rights reserved.


Salunkhe P.R.,SASMIRA | Nayak S.S.,Karmaveer Bhaurao Patil College
Man-Made Textiles in India | Year: 2012

The synthesis of nanoparticles has been entirely a chemical process since decades. Owing to environmental concerns the development of eco-friendly processes for synthesis of nanomaterials is the need of the day. One approach that shows great potential is synthesis of nanoparticles using micro-organisms such as bacteria, yeast and fungi. In this article, isolation of a silver resistant Pseudomonas species from atmospheric air is reported. The product was isolated and tested for silver nanoparticle synthesis by exposing it to various concentrations of silver nitrate in liquid media. Silver nanoparticles (AgNPs) synthesis was confirmed by various analytical techniques. The application of the product developed to impart antibacterial properties to textiles gave encouraging results.


Pawar K.,Karmaveer Bhaurao Patil College | Rokade K.,Shri Vijaysinha Yadav Arts and Science College | Mali G.,Bharati Vidyapeeths M.B.S.K. Kanya Mahavidyalaya
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2013

The present investigation describes about the antibacterial activity of three different natural honey samples obtained from different locations of Sangli district. The pH values of these collected honey samples ranges from 4.0 to 4.5. Antibacterial activity of honey at its natural acidic pH as well as by maintaining neutral pH was studied on seven pathogenic bacteria. Marked variations were observed in the antibacterial activity of these honey samples. All the honey samples at natural acidic pH as well as at neutral pH showed a spectrum of antibacterial activity on five different bacterial species such as Salmonella typhi, Shigella dysentriae, Bacillus megaterium, Staphylococcus aureus, and Proteus vulgaris. © Global Science Publications.


Ghorpade R.,Karmaveer Bhaurao Patil College | Balasubramanya R.H.,Institute of Chemical Technology
Man-Made Textiles in India | Year: 2013

Banana belongs to the family Musaceae of the genus Musa. The banana pseudostem which yields the fibers is considered to be an agricultural waste; so utilization of the pseudostem to extract the fibers by microbial retting is done. The pseudostem is retted anaerobically with the microbial consortium for the specific length of time to remove lignin and pectin to separate the fibers from plant tissue. The microbes involved in retting were isolated and identified. The extracted fibers have been studied for its physico-chemical and mechanical properties. The retted fibers were used in preparation of yarn, nonwoven fabric, blending with jute fabric and handicrafts.


Mahajan P.M.,Institute of Chemical Technology | Nayak S.,Karmaveer Bhaurao Patil College | Lele S.S.,Institute of Chemical Technology
Journal of Bioscience and Bioengineering | Year: 2012

Fibrinolytic enzymes are important in treatment of cardiovascular diseases. The present work reports isolation, screening and identification of marine cultures for production of fibrinolytic enzymes. A potent fibrinolytic enzyme-producing bacterium was isolated from marine niches and identified as Bacillus subtilis ICTF-1 on the basis of the 16S rRNA gene sequencing and biochemical properties. Further, media optimization using L18-orthogonal array method resulted in enhanced production of fibrinolytic enzyme (8814U/mL) which was 2.6 fold higher than in unoptimized medium (3420U/mL). In vitro assays revealed that the enzyme could catalyze blood clot lysis effectively, indicating that this enzyme could be a useful thrombolytic agent. A fibrinolytic enzyme was purified from the culture supernatant to homogeneity by three step procedures with a 34.42-fold increase in specific activity and 7.5% recovery. This purified fibrinolytic enzyme had molecular mass of 28kDa, optimal temperature and pH at 50°C and 9, respectively. It was stable at pH 5.0-11.0 and temperature of 25-37°C. The enzyme activity was activated by Ca2+ and obviously inhibited by Zn2+, Fe3+, Hg2+ and PMSF. The purified fibrinolytic enzyme showed high stability towards various surfactants and was relatively stable towards oxidizing agent. Considering these properties purified fibrinolytic enzyme also finds potential application in laundry detergents in addition to thrombolytic agent. The gene encoding fibrinolytic enzyme was isolated and its DNA sequence was determined. Compared the full DNA sequence with those in NCBI, it was considered to be a subtilisin like serine-protease. © 2011 The Society for Biotechnology, Japan.


Mote U.S.,Karmaveer Bhaurao Patil College | Anbhule P.V.,Shivaji University | Kolekar G.B.,Shivaji University
Journal of Surfactants and Detergents | Year: 2016

The fluorescence resonance energy transfer (FRET) from tyrosine to pyridoxine hydrochloride in different surfactant solutions and in deionized water has been investigated by using a fluorescence spectroscopic technique. The Stern–Volmer quenching constant, the distance between donor (tyrosine) and acceptor (pyridoxine hydrochloride), and the energy transfer efficiency were obtained using the Stern–Volmer relation and Forster’s theory of nonradiative energy transfer. The data obtained from the measurement reveals that the FRET occurs more effectively in aqueous SDS micellar solution than in CTAB or linear alcohol ethoxylate micellar systems and deionized water. Moreover, the binding constant, number of binding sites, and thermodynamic parameters ∆G, ∆H, ∆S were obtained by studying the tyrosine-pyridoxine hydrochloride interactions at three different temperatures. The decrease in binding constant with temperature reflects the existence of weak interaction between tyrosine and pyridoxine hydrochloride at high temperature. The analysis confirms that van der Waals forces and hydrogen bonding are involved in the interaction. © 2016, AOCS.


Mahajan G.B.,Piramal Life science Ltd | Balachandran L.,Karmaveer Bhaurao Patil College
Frontiers in Bioscience - Elite | Year: 2012

The discovery of Penicillin in 1928 and that of Streptomycin in 1943, has been pivotal to the exploration of nature as a source of new lead molecules. Globally, the microbiologist today is acknowledged as a crucial player in the drug discovery program. The microbial products, especially those from actinomycetes have been a phenomenal success for the past seven decades. Bioprospecting for new leads are often compounded by the recurrence of known antibiotics in newer microbial isolates. Despite all these deterrents, actinomycetes have proved to be a sustained mine of novel antibiotics, which selectively destroys the pathogens without affecting the host tissues. Each of these antibiotics is unique in their mode of action. Their versatility and immense economic value is something, which is extremely noteworthy. The anti-infective turn-over of over 79 billion US dollars in 2009, includes about 166 antibiotics and derivatives such as the Beta-lactam peptide antibiotics, the macrolide polyketide erythromycin, tetracyclines, aminoglycosides, daptomycin, tigecycline, most of which are produced by actinomycetes (1). Actinomycetes continue to play a highly significant role in drug discovery and development. Among the bioactive compounds that have been obtained so far from microbes, 45% are produced by actinomycetes, 38% by fungi and 17% by unicellular eubacteria (2). Further many chemically synthesized drugs owe their origin to natural sources. In this review article, we highlight the recent antibiotics from actinomycetes with emphasis on their source, structures, activity, mechanism of action and current status.

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