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Shi M.-D.,Kaohsiung Veterans General Hospital Tainan Branch | Chao J.-K.,Yuli Veterans Hospital | Ma M.-C.,National Cheng Kung University | Hao L.-J.,Kaohsiung Veterans General Hospital Tainan Branch | Chao I.-C.,Chinese Institute of Clinical Medicine
Journal of Sexual Medicine | Year: 2014

Introduction: Obesity has been receiving an increasing amount of attention recently, but investigations regarding the potential impact of obesity, sexual behaviors, and sex hormones on erectile dysfunction (ED) in men have not completely clarified the association. Aim: To identify the relationship between ED, sexual behavior, sexual satisfaction, sex hormones, and obesity in older adult males in Taiwan. Methods: Data were obtained from a baseline survey of 476 older adult males (≧40 years old). Their demographic data, body mass index (BMI), sex hormones, sexual desire, sexual satisfaction, and ED status were assessed. Main Outcome Measures: The International Index of Erectile Function-5 (IIEF-5), Sexual Desire Inventory (SDI), and Sexual Satisfaction Scale (SSS) were used to assess ED, sexual desire, and sexual satisfaction. Results: In all, 476 men were available for analysis. The mean age of the sample was 51.34±7.84 years (range 40 to 70 years). The IIEF total score had a mean of 19.44±4.98; 264 (55.5%) subjects had ED, 250 (52.9%) were currently obese (BMI≧27), and 297 (62.4%) had metabolic syndrome. The results showed an increased risk of ED among obese men and subjects with lower levels of sex hormones and lower sexual desire. Testosterone levels were lower in subjects with obesity (P<0.001). Among the predictors of ED, obesity (odds ratio [OR]=1.62, 95% CI=1.07-2.44, P=0.021), abnormal high sensitivity C-reactive protein (hs-CRP) (OR=10.59, 95% CI=4.70-23.87, P<0.001), and lower serum full testosterone (OR=3.27, 95% CI=2.16-4.93, P<0.001) were significantly independent factors. Conclusions: This study supports the idea of a close relationship between low levels of sex hormones, sexual desire, sexual satisfaction, obesity, and ED, and also shows that low free testosterone and hs-CRP may predict ED, even in obese populations. © 2013 International Society for Sexual Medicine.


Wang S.-C.,Kaohsiung Veterans General Hospital Tainan Branch | Yu C.-L.,Kun Shan University
Aging and Mental Health | Year: 2015

Objectives: The purpose was to examine the effectiveness of music care on cognitive function, depression, and behavioral problems among elderly people with dementia in long-term care facilities in Taiwan. Methods: The study had a quasi-experimental, longitudinal research design and used two groups of subjects. Subjects were not randomly assigned to experimental group (n = 90) or comparison group (n = 56). Based on Bandura's social cognition theory, subjects in the experimental group received Kagayashiki music care (KMC) twice per week for 24 weeks. Subjects in the comparison group were provided with activities as usual. Results: Results found, using the control score of the Clifton Assessment Procedures for the Elderly Behavior Rating Scale (baseline) and time of attending KMC activities as a covariate, the two groups of subjects had statistically significant differences in the mini-mental state examination (MMSE). Results also showed that, using the control score of the Cornell Scale for Depression in Dementia (baseline) and MMSE (baseline) as a covariate, the two groups of subjects had statistically significant differences in the Clifton Assessment Procedures for the Elderly Behavior Rating Scale. Conclusion: These findings provide information for staff caregivers in long-term care facilities to develop a non-invasive care model for elderly people with dementia to deal with depression, anxiety, and behavioral problems. © 2015 Taylor & Francis


PubMed | China Institute of Technology, Kaohsiung Veterans General Hospital, Tajen University, St Joseph Hospital and 2 more.
Type: Journal Article | Journal: The Chinese journal of physiology | Year: 2016

This study explored the effect of deltamethrin, a pesticide, on intracellular free Ca concentration ([Ca]i) in PC3 human prostate cancer cells. Deltamethrin at concentrations between 5 M and 20 M evoked [Ca]i rises in a concentration-dependent manner. This Ca signal was inhibited by 22% by removal of extracellular Ca. Nifedipine, econazole, and SKF96365 also inhibited the Ca signal. Treatment with the endoplasmic reticulum Ca pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) in Ca-free medium nearly abolished deltamethrin-induced [Ca]i rises. Treatment with deltamethrin also inhibited most of BHQ-induced [Ca]i rises. Inhibition of phospholipase C (PLC) with U73122 failed to alter deltamethrin-evoked [Ca]i rises. Deltamethrin killed cells at concentrations of 20-100 M in a concentration-dependent fashion. Chelation of cytosolic Ca with 1,2-bis (2-aminophenoxy) ethane-N, N, N, N-tetraacetic acid/acetoxymethyl ester (BAPTA/AM) did not prevent deltamethrins cytotoxicity. Together, in PC3 human prostate cancer cells, deltamethrin induced [Ca]i rises that involved Ca entry through store-operated Ca channels and PLC-independent Ca release from the endoplasmic reticulum. Deltamethrin induced cytotoxicity in a Ca-independent manner.


Lee Y.-Y.,I - Shou University | Lee Y.-Y.,Kaohsiung Veterans General Hospital Tainan Branch | Lo C.-T.,Fooyin University | Sheu S.-J.,Kaohsiung Veterans General Hospital | And 2 more authors.
Investigative Ophthalmology and Visual Science | Year: 2013

Purpose. We investigated the independent impact of potential risk factors on myopia in young adults. Methods. A survey study was conducted with male military conscripts aged 18 to 24 years between February 2010 and March 2011 in Taiwan. The participants were examined using non-cycloplegic autorefraction and biometry. The participants provided data about potential risk factors, including age, parental myopia, education, near work, outdoor activity, and urbanization. Myopia was defined as the mean spherical equivalent of the right eye of ≤0.5 diopters (D). Results. Among 5145 eligible participants, 5048 (98.11%) had refraction and questionnaire data available; 2316 (45.88%) of these received axial length examination. The prevalence of myopia was 86.1% with a mean refractive error of -3.66 D (SD = 2.73) and an axial length of 25.40 mm (SD = 1.38). Older age, having myopic parents, higher education level, more time spent reading, nearer reading distance, less outdoor activity, and higher urbanization level were associated with myopia and longer axial length. More computer use was related to longer axial length. All risk factors associated with myopia also were predictors of high myopia (≤-6.0 D), with the exception of outdoor activity. Finally, an interaction analysis showed shorter axial length was associated with more time spent outdoors only at high urbanization level. Conclusions. Older age, parental myopia, higher education level, more near work, less outdoor activity, and higher urbanization level were independent predictors of myopia. These data provided evidence to the multifactorial nature of myopia in young men in Taiwan. © 2013 The Association for Research in Vision and Ophthalmology, Inc.


Chien S.-T.,Kaohsiung Armed Forces General Hospital | Chien S.-T.,Fooyin University | Shi M.-D.,Kaohsiung Veterans General Hospital Tainan Branch | Shi M.-D.,Chung Hwa University of Medical Technology | And 3 more authors.
Cancer Cell International | Year: 2015

Background: Galangin (3,5,7-trihydroxyflavone) is a flavonoid compound found in high concentration in lesser galangal. The objective of this study was to investigate the ability of galangin to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced the invasion and metastasis of HepG2 liver cancer cells. Results: First, using a cell-matrix adhesion assay, immunofluorescence assay, transwell-chamber invasion/migration assay, and wound healing assay, we observed that galangin exerted an inhibitory effect on TPA-induced cell adhesion, morphology/actin cytoskeleton arrangement, invasion and migration. Furthermore, the results of gelatin zymography and reverse transcriptase polymerase chain reaction (RT-PCR) assays showed that galangin reduced the TPA-induced enzyme activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in HepG2 cells; moreover, the messenger RNA level was downregulated. We also observed through a Western blotting assay that galangin strongly inhibited the TPA-induced protein expressions of protein kinase Cα (PKCα), protein kinase Cδ (PKCδ), phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2), the phospho-inhibitor of kappaBα (phospho-IΚBα), c-Fos, c-Jun, and nuclear factor kappa B (NF-ΚB). Next, galangin dose-dependently inhibited the binding ability of NF-ΚB and activator protein 1 (AP-1) to MMP-2/MMP-9 promoters, respectively, resulting in the suppression of MMP-2/MMP-9 enzyme activity. Conclusions: The results revealed that galangin effectively inhibited the TPA-induced invasion and migration of HepG2 cells through a protein kinase C/extracellular signal-regulated kinase (PKC/ERK) pathway. Thus, galangin may have widespread applications in clinical therapy as an anti-metastatic medicament. © Chien et al.


Shi M.-D.,Kaohsiung Medical University | Shi M.-D.,Kaohsiung Veterans General Hospital Tainan Branch | Shi M.-D.,Chung Hwa University of Medical Technology | Shih Y.-W.,Chung Hwa University of Medical Technology | And 3 more authors.
Cell Biochemistry and Biophysics | Year: 2013

α-Tomatine, isolated from Lycopersicon esculentum Linn., is a naturally occurring glycoalkaloids in immature green tomatoes. Some reports demonstrated that α-tomatine had various anti-carcinogenic properties. First, the result demonstrated α-tomatine could inhibit TPA-induced the abilities of the adhesion, morphology/actin cytoskeleton arrangement, invasion, and migration by cell-matrix adhesion assay, immunofluorescence stain assay, Boyden chamber invasion assay, and wound-healing assay. Data also showed α-tomatine could inhibit the activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) and protein kinase C-α (PKCα) involved in the downregulation of the enzyme activities and messenger RNA levels of matrix metalloproteinase-2/9 (MMP-2/MMP-9) induced by TPA. Next, α-tomatine also strongly inhibited TPA-induced the activation of nuclear factor kappa B (NF-κB) and phospho-inhibitor of kappa Bα (phospho-IκBα). In addition, TPA-induced translocation of PKC-α from cytosol to membranes, and suppression of TPA elicited the expression of PKC-α by adding the PKC-α inhibitors, GF-109203X and Gö-6983. The treatment of specific inhibitor for ERK (U0126) to MCF-7 cells could inhibit TPA-induced MMP-2/MMP-9 and phospho-ERK along with an inhibition on cell invasion and migration. Application of α-tomatine to prevent the invasion/migration of MCF-7 cells through blocking PKCα/ERK/NF-κB activation is first demonstrated herein. © 2012 Springer Science+Business Media New York.


Lee Y.-Y.,I - Shou University | Lee Y.-Y.,Kaohsiung Veterans General Hospital Tainan Branch | Lo C.-T.,Fooyin University | Sheu S.-J.,Kaohsiung Veterans General Hospital | And 2 more authors.
Ophthalmic Epidemiology | Year: 2015

Purpose: To investigate the association between potential risk factors for myopia and its progression in young adult Taiwanese men. Methods: A survey of male military conscripts (aged 18-24 years) was conducted from February 2010 to March 2011 in Taiwan. Participants underwent comprehensive eye examinations, including measurements of axial length and corneal radius by optical biometry and non-cycloplegic autorefraction. Participants also provided self-reported progression of myopia and information regarding potential risk factors, including age, parental myopia, educational level, close work, outdoor activities, and urbanization. Results: Of 5145 eligible participants, 5048 (98.11%) provided refraction and questionnaire data; 2316 (45.88%) of the 5048 also had biometric measurements. The prevalence of myopia was 86.1% in this group, with a mean refractive error of -3.66 diopters (D). Of the 5048 participants, 1376 (27.3%) had experienced progression of their myopia during the past year. There were trends for a higher prevalence of myopia among older participants (p = 0.014), those with a history of parental myopia (p<0.001), higher levels of education (p = 0.001), increased time spent reading (p<0.001), less time outdoors (p=0.003), and higher levels of urbanization (p = 0.010). However, only parental myopia, close work, and higher urbanization levels were significantly associated with self-reported progression of myopia. Conclusion: Older age, parental myopia, higher educational level, close work, fewer outdoor activities, and higher urbanization level were associated with the prevalence of myopia in Taiwanese men. © Informa Healthcare USA, Inc.


Chen K.-S.,Chung Hwa University of Medical Technology | Shi M.-D.,Kaohsiung Veterans General Hospital Tainan Branch | Shi M.-D.,Chung Hwa University of Medical Technology | Chien C.-S.,Chi Mei Medical Center | Shih Y.-W.,Chung Hwa University of Medical Technology
Cell and Bioscience | Year: 2014

Background: Pinocembrin is the most abundant flavonoid in propolis. In this study, we investigated the antimetastatic effect of pinocembrin on TGF-β1-induced epithelial-mesenchymal transition (EMT) and metastasis of human Y-79 retinoblastoma cells.Results: Firstly, the results showed that pinocembrin significantly suppresses the TGF-β1-induced abilities of the invasion and migration of Y-79 cells under non-cytotoxic concentration. Pinocembrin decreased TGF-β1-induced expression of vimentin, N-cadherin, αv and β3 integrin in Y-79 cells. Molecular data also showed pinocembrin inhibits the activation of focal adhesion kinase (FAK) and p38α signal involved in the downregulation of enzyme activities, protein and messenger RNA levels of matrix metalloproteinase-2/9 (MMP-2/-9) induced by TGF-β1. Next, pinocembrin also strongly inhibited the degradation of inhibitor of kappaBα (IκBα) and the nuclear levels of nuclear factor kappa B (NF-κB). Also, a dose-dependent inhibition on the binding ability of NF-κB was further observed under pinocembrin treatment.Conclusions: Presented results indicated that pinocembrin inhibits TGF-β1-induced epithelial-mesenchymal transition (EMT) and metastasis of Y-79 cells by inactivating the αvβ3 integrin/FAK/p38α signaling pathway. Thus, our findings point to the anticancer potential of pinocembrin against retinoblastoma cells. © 2014 Chen et al.; licensee BioMed Central Ltd.


Shi M.-D.,Kaohsiung Medical University | Shi M.-D.,Kaohsiung Veterans General Hospital Tainan Branch | Shi M.-D.,Chung Hwa University of Medical Technology | Liao Y.-C.,Chi Mei Medical Center | And 2 more authors.
Phytomedicine | Year: 2013

Hepatocyte growth factor (HGF), and its receptor, c-Met activation has recently been shown to play important roles in cancer invasion and metastasis in a wide variety of tumor cells. We use HGF as an invasive inducer of human HepG2 cells to investigate the effect of four flavones including apigenin, tricetin, tangeretin, and nobiletin on HGF/c-Met-mediated tumor invasion and metastasis. Among them, nobiletin markedly inhibited HGF-induced the abilities of the adhesion, invasion, and migration by cell-matrix adhesion assay and transwell-chamber invasion/migration assay under non-cytotoxic concentrations. Data also showed nobiletin inhibited HGF-induced cell scattering and cytoskeleton changed such as filopodia and lamellipodia. Furthermore, nobiletin could inhibit HGF-induced the membrane localization of phosphorylated c-Met, ERK2, and Akt, but not phosphorylated JNK1/2 and p38. Next, nobiletin significantly decreased the levels of phospho-ERK2 and phospho-Akt in ERK2 or Akt siRNA-transfected cells concomitantly with a marked reduction on cell invasion and migration. In conclusion, nobiletin attenuates HGF-induced HepG2 cells metastasis involving both ERK and PI3K/Akt pathways and are potentially useful as anti-metastatic agents for the treatment of hepatoma. © 2013 Elsevier GmbH. All rights reserved.


Chuang Y.-C.,National Cheng Kung University | Lin Y.-S.,National Cheng Kung University | Liu C.-C.,National Cheng Kung University | Liu H.-S.,National Cheng Kung University | And 4 more authors.
Journal of the Formosan Medical Association | Year: 2013

Hemorrhage is one of the hallmarks of dengue hemorrhagic fever. However, the mechanisms that cause hemorrhage are unclear. In this review we focus on the possible factors that may be involved in the disturbance of coagulation and fibrinolysis during dengue virus (DENV) infection. Factors such as autoantibodies and cytokines induced by DENV infection as well as hemostatic molecules expressed on DENV-infected cells, and DENV viral proteins may all contribute to the defect of hemostasis during DENV infection. It is the combination of these viral and host factors that may tilt the balance of coagulation and fibrinolysis toward bleeding in dengue patients. © 2012.

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