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Horng C.-T.,Kaohsiung Armed Force General Hospital | Chiang N.-N.,Kaohsiung Veterans General Hospital Pingtung Branch | Chen I.-L.,Tajen University | Liang W.-Z.,Kaohsiung Veterans General Hospital | And 4 more authors.
Journal of Receptors and Signal Transduction | Year: 2013

Clotrimazole is an antimycotic imidazole derivative that interferes with cellular Ca2+ homeostasis. This study examined the effect of clotrimazole on cytosolic Ca2+ concentrations ([Ca2+]i) and viability in HA59T human hepatoma cells. The Ca2+-sensitive fluorescent dye fura-2 was applied to measure [Ca2+]i. Clotrimazole induced [Ca2+]i rises in a concentration-dependent manner. The response was reduced by removing extracellular Ca2+. Clotrimazole-evoked Ca2+ entry was suppressed by store-operated channel inhibitors (nifedipine, econazole and SK&F96365) and protein kinase C modulators (GF109203X and phorbol, 12-myristate, 13-acetate). In Ca 2+-free medium, incubation with the endoplasmic reticulum Ca 2+ pump inhibitor 2,5-di-tert-butylhydroquinone abolished clotrimazole-induced [Ca2+]i rise. Inhibition of phospholipase C with U73122 abolished clotrimazole-induced [Ca2+]i rise. At 10-40 M, clotrimazole inhibited cell viability, which was not reversed by chelating cytosolic Ca2+. Clotrimazole at 10 and 30 M also induced apoptosis. Collectively, in HA59T cells, clotrimazole-induced [Ca2+]i rises by evoking phospholipase C-dependent Ca2+ release from the endoplasmic reticulum and Ca2+ entry via store-operated Ca2+ channels. Clotrimazole also caused apoptosis. © 2013 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. Source


Kuo C.-Y.,National Defense Medical Center | Huang B.-R.,National Defense Medical Center | Chen H.-C.,National Defense Medical Center | Shih C.-P.,National Defense Medical Center | And 6 more authors.
BioMed Research International | Year: 2015

The aim of this study was to retrospectively review the long-term hearing results and the impact of mastoid exclusion/obliteration in patients with cholesteatoma (102 ears) who underwent retrograde tympanomastoidectomy and in whom bone chips/paté were applied as the sole materials during the procedure. In 79 ears, this was combined with ossiculoplasty in a single-stage procedure. In >71% of ears, the results of audiometric testing were monitored for more than 2 years. The results suggested there was a significant gain in hearing following surgery, with respect to the postoperative change in both air-conduction thresholds and air-bone gaps (P < 0.001). Linear regression analyses of pure-tone averages at different frequencies, before and after surgery, demonstrated that patients benefitted from a postoperative hearing gain at low and middle frequencies, but their hearing often deteriorated at frequencies of 8000 Hz. As for the impact of the type of tympanoplasty on hearing outcomes, type III-interposition markedly increased hearing gain. The overall rate of postoperative adverse events was 8.8%. We conclude that reconstruction of the ear canal and mastoid via mastoid exclusion/obliteration using bone chips/paté can be considered as an alternative procedure following retrograde mastoidectomy. It gives excellent surgical results and has fewer postoperative adverse events. © 2015 Chao-Yin Kuo et al. Source


Chiang N.-N.,Kaohsiung Veterans General Hospital Pingtung Branch | Horng C.-T.,Kaohsiung Armed Force General Hospital | Chang S.S.,Tajen University | Lee C.-F.,Kaohsiung Veterans General Hospital Pingtung Branch | And 3 more authors.
Life Science Journal | Year: 2013

This study was to evaluate the hypolipidemic activity of Polygonatum alte-lobatum Hayata extract (PAHE) in hamsters fed with high-fat diet (HFD). Experimentally induced hyperlipidemia was produced by feeding hamsters with HFD for 14 days. Hypercholesterolemic hamsters were administered gavagely with PAHE at the dose of 300 and 750 mg/kg bw along with HFD for 28 days in order to estimate their hypolipidemic activity. The lipid profile and histopathological studies were carried out at the end of experiment. Supplementation with PAHE resulted in hypolipidemic effect by lowering the serum lipid parameters such as significant decrease in total cholesterol (TC), low-density lipoprotein cholesterol (LDL) and very low-density lipoprotein cholesterol (VLDL), and increase in high-density lipoprotein cholesterol (HDL). Significant decrease of TC and triglyceride (TG) was also found in liver after administration of PAHE for 28 days. Histopathological findings in hamster liver supported the effect of PAHE on reduction of HFD-induced hepatic steatosis. The consumption of P. alte-lobatum may act as a functional food with ameliorating hyperlipidemia. Source


Chen Y.-L.,Kaohsiung Armed Force General Hospital | Tzeng D.-S.,Kaohsiung Armed Force General Hospital | Tzeng D.-S.,Hyperbaric and Undersea Institute | Cheng T.-S.,Kaohsiung Armed Force General Hospital | Lin C.-H.,Calo Psychiatric Center
Annals of General Psychiatry | Year: 2012

Background: The objective of this study was to assess sentinel event analysis and relative factors in different mental healthcare settings. In addition, the occurrence of sentinel events in different hospital settings was compared and potential risk factors contributing to sentinel events identified.Methods: A total of 75 consecutive adult subjects were enrolled from 2 psychiatric units, 1 within a general hospital and 1 at a psychiatric hospital in southern Taiwan. A retrospective chart review of the psychiatric inpatients was conducted for patients that met the criteria for a sentinel event between July 2004 and May 2011. A comparison of the hospital settings was made and differences between suicidal and non-suicidal sentinel events studied.Results: Psychiatric patients that received general hospital psychiatric services (1) appeared to experience a sentinel event soon after admission, (2) the time between the sentinel event occurrence and patient death was shorter, (3) there was a higher probability of potential medical illness than among inpatients treated at a specialized psychiatric hospital, (4) the sentinel event subjects that committed suicide were younger, had a shorter hospital stay, shorter time to occurrence of the sentinel event followed by an unexpected death than the non-suicidal group, and (5) a younger age, higher education level, previous suicide attempt and family psychiatric history were important predictors of suicide among psychiatric inpatients.Conclusions: The results of this study suggest that psychiatric inpatients treated at a general hospital require careful examination for potential physical illness and greater efforts to prevent suicide. A younger age, higher education level, history of a previous suicide attempt and family psychiatric history are additional risk factors for suicide among these patients. © 2012 Chen et al; licensee BioMed Central Ltd. Source


Chen P.C.,I - Shou University | Kuo S.M.,I - Shou University | Jao J.C.,Kaohsiung Medical University | Yang S.W.,Kaohsiung Medical University | And 3 more authors.
Aesthetic Plastic Surgery | Year: 2016

Background: Capsular contracture is the most common complication of breast augmentation. Although numerous procedures are intended to prevent capsular contracture, their efficacy does not satisfy surgeons or patients. In the present study, we used shock waves to develop innovative protocols to treat capsular contracture in rabbits. Methods: We used shock waves to treat capsular contracture in a rabbit model. Six clinical parameters were evaluated to determine the treatment efficacy of shock waves on the pathological histology of capsular contracture. Dual-flip-angle T1-mapping magnetic resonance imaging was used to confirm the pathological findings. Results: Among the parameters, myxoid change, vascular proliferation, and lymphoplasma cell infiltration around the capsule increased more after treatment than they did in a control group. Capsular thickness, inner thinner collagen layer, and capsule wall collagen deposition decreased after shock wave treatment; only the inner thinner collagen layer and capsule wall collagen deposition changed significantly. The MRI findings for both scar thickness and water content were consistent with pathological biology findings. Conclusion: This was the first pilot study and trial to treat capsular contractures using shock waves. We found that shock waves can cause changes in the structure or the composition of capsular contracture. We conclude that the treatment could decrease water content, loosen structure, decrease collagen deposition, and might alleviate scar formation from capsular contracture. We believe that the treatment could be a viable remedy for capsular contractures. No Level Assigned: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. © 2016, Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery. Source

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