Kaohsiung Armed Force General Hospital
Kaohsiung Armed Force General Hospital
Lin H.-C.,Taipei Veterans General Hospital |
Lin H.-C.,Kaohsiung Armed Force General Hospital |
Chen C.-L.,Taipei Veterans General Hospital |
Lin H.-S.,Fooyin University |
And 10 more authors.
Clinical Endocrinology | Year: 2014
Objective Secondary hyperparathyroidism and its associated abnormalities in mineral metabolism and haemodynamic changes increase the cardiovascular risk in patients with end-stage renal disease (ESRD). Our objective was to determine the association of parathyroidectomy (PTX) with major cardiovascular events in nondiabetic dialysis patients with severe secondary hyperparathyroidism (SHPTH). Design and Patients We performed a cohort study with fifty-three nondiabetic ESRD patients who were treated with maintenance haemodialysis and who had intact parathyroid hormone (PTH) levels > 800 pg/ml. Participants received either only medical therapy or medical therapy and total PTX with autotransplantation for SHPTH. Measurements We evaluated the associations between PTX and major cardiovascular events including death, cerebrovascular accident and myocardial infarction. The biochemical and haemodynamic changes associated with PTX were measured. Results During the mean follow-up of 72 months, twenty-three patients received only medical treatment (medical group) while thirty patients underwent PTX in addition to medical treatment (PTX group). The two groups were comparable in respect of baseline characteristics. PTX group was found to be associated with a reduced incidence of major cardiovascular events (P = 0·021). A multiple Cox regression analysis showed that the variable significantly associated with major cardiovascular events was treatment modality (medical therapy vs medical therapy and parathyroidectomy, hazard ratio = 26·12, 95% CI = 1·30-526·27, P = 0·033). Blood pressure, haemoglobin, alkaline phosphatase, calcium, phosphate and calcium × phosphate product significantly improved after PTX. Conclusions PTX was associated with better cardiovascular outcome in nondiabetic dialysis patients with severe SHPTH. © 2013 John Wiley & Sons Ltd.
PubMed | Kaohsiung Veterans General Hospital, National Sun Yat - sen University, Kaohsiung Chang Gung Memorial Hospital, National Museum of Marine Biology and Aquarium and 2 more.
Type: Journal Article | Journal: The Prostate | Year: 2016
Prostate cancer is one of the most prevalent cancers in men worldwide. Aberrant activation of c-Met/signal transducer and activator of transcription-3 (STAT3) signaling is involved in prostate carcinogenesis, underscoring the demand for developing c-Met/STAT3-targeting drugs. Thus, we first utilized virtual screening strategy to identify STAT3-inhibiting marine compound, heteronemin, and then validated the STAT3-inhibiting function of heteronemin in prostate cancer cells.Human prostate cancer LNCaP, DU145, and PC-3 cell lines were treated with heteronemin for 24hr, then the cell viability was evaluated by MTT assay. Flow cytometry was performed to analyze the apoptosis in heteronemin-treated cells. Western blot and quantitative real-time PCR were executed to further confirm the c-Met/STAT3 signaling inhibition by heteronemin in DU145 and PC-3 cells.In this study, we employed the virtual screening strategy to identify heteronemin, a spongean sesterterpene, as a potential STAT3 inhibitor from Taiwan marine drugs library. Application of heteronemin potently suppressed the viability and anchorage-independent growth of human prostate cancer cells. Besides, heteronemin induced apoptosis in prostate cancer cells by activation of both intrinsic (caspase-9) and extrinsic (caspase-8) apoptotic pathways. By luciferase assay and expression analysis, it was confirmed that heteronemin inhibited the phosphorylation of c-Met/src/STAT3 signaling axis, STAT3-driven luciferase activities and expression of STAT3-regulated genes including Bcl-xL, Bcl-2, and Cyclin D1. Finally, heteronemin effectively antagonized the hepatocyte growth factor (HGF)-stimulated c-Met/STAT3 activation as well as the proliferation and colonies formation in refractory prostate cancer cells.These findings suggest that heteronemin may constitute a novel c-Met/STAT3-targeting agent for prostate cancer. Prostate 76:1469-1483, 2016. 2016 Wiley Periodicals, Inc.
PubMed | Tajen University, China Institute of Technology, University of Science and Technology of China, Kaohsiung Armed Force General Hospital and Kaohsiung Veterans General Hospital
Type: Journal Article | Journal: The Chinese journal of physiology | Year: 2015
Methoxychlor, an organochlorine pesticide, is thought to be an endocrine disrupter that affects Ca homeostasis and cell viability in different cell models. This study explored the action of methoxychlor on cytosolic free Ca concentrations ([Ca]i) and apoptosis in HA59T human hepatoma cells. Fura-2, a Ca-sensitive fluorescent dye, was applied to measure [Ca]i. Methoxychlor at concentrations of 0.1-1 M caused a [Ca]i rise in a concentration-dependent manner. Removal of external Ca abolished methoxychlors effect. Methoxychlor-induced Ca influx was confirmed by Mn-induced quench of fura-2 fluorescence. Methoxychlor-induced Ca entry was inhibited by nifedipine, econazole, SK&F96365, and protein kinase C modulators. Methoxychlor killed cells at concentrations of 10-130 M in a concentration-dependent fashion. Chelation of cytosolic Ca with 1,2-bis(2-aminophenoxy) ethane-N,N,N,N-tetraacetic acid/AM (BAPTA/AM) did not prevent methoxychlors cytotoxicity. Methoxychlor (10 and 50 M) induced apoptosis concentration-dependently as determined by using Annexin V/propidium iodide staining. Together, in HA59T cells, methoxychlor induced a [Ca]i rise by inducing Ca entry via protein kinase C-sensitive Ca-permeable channels, without causing Ca release from stores. Methoxychlor also induced apoptosis that was independent of [Ca]i rises.
Jao J.-C.,Kaohsiung Medical University |
Mac K.-W.,Kaohsiung Medical University |
Chang C.-Y.,Kaohsiung Medical University |
Wu Y.-C.,Kaohsiung Armed Force General Hospital |
And 2 more authors.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2017
This study aimed to investigate the VX2 tumor growth in rabbit liver using T2-weighted imaging (T2WI) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Five New Zealand white (NZW) rabbits were implanted with VX2 cell suspension in liver. Afterwards, MRI was performed 7, 14, 21 and 28 days after tumor implantation. A 1.5T clinical MRI scanner was used to perform scans. After 3-plane localizer, T1 weighted imaging (T1WI), T2WI, and DCE-MRI using a three-dimensional gradient echo pulse sequence was performed. After 4 pre-contrast images were acquired, each rabbit was injected i.v. with 0.1 mmol/kg Dotarem. The total scan time after Dotarem administration was 30 minutes. All acquired images were analyzed using ImageJ software. Several regions of interest were selected from the rims of tumor, liver, and muscle. The enhancement ratio (ER) was calculated by dividing the MR signal after Dotarem injection to the MR signal before Dotarem injection. The maximum ER (ER-max) value of tumor for each rabbit was observed right after the Dotarem injection. The T2W MR signal intensities (T2W-SI) and the ER-max values obtained 7, 14, 21 and 28 days after tumor implantation were analyzed with a linear regression algorithm. Both T2W-SI and ER-max of tumors increased with time. The changes for T2W-SI and ER-max of tumors between 7 and 28 days after tumor implantation were 32.66% and 18.14%, respectively. T2W-SI is more sensitive than ER-max for monitoring the growth of VX2 tumor in a rabbit liver model. © 2017 SPIE.
PubMed | I - Shou University, Kaohsiung Medical University and Kaohsiung Armed Force General Hospital
Type: Journal Article | Journal: Aesthetic plastic surgery | Year: 2016
Capsular contracture is the most common complication of breast augmentation. Although numerous procedures are intended to prevent capsular contracture, their efficacy does not satisfy surgeons or patients. In the present study, we used shock waves to develop innovative protocols to treat capsular contracture in rabbits.We used shock waves to treat capsular contracture in a rabbit model. Six clinical parameters were evaluated to determine the treatment efficacy of shock waves on the pathological histology of capsular contracture. Dual-flip-angle T1-mapping magnetic resonance imaging was used to confirm the pathological findings.Among the parameters, myxoid change, vascular proliferation, and lymphoplasma cell infiltration around the capsule increased more after treatment than they did in a control group. Capsular thickness, inner thinner collagen layer, and capsule wall collagen deposition decreased after shock wave treatment; only the inner thinner collagen layer and capsule wall collagen deposition changed significantly. The MRI findings for both scar thickness and water content were consistent with pathological biology findings.This was the first pilot study and trial to treat capsular contractures using shock waves. We found that shock waves can cause changes in the structure or the composition of capsular contracture. We conclude that the treatment could decrease water content, loosen structure, decrease collagen deposition, and might alleviate scar formation from capsular contracture. We believe that the treatment could be a viable remedy for capsular contractures.This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
PubMed | Tri Service General Hospital, Kaohsiung Medical University and Kaohsiung Armed Force General Hospital
Type: | Journal: BioMed research international | Year: 2015
The aim of this study was to retrospectively review the long-term hearing results and the impact of mastoid exclusion/obliteration in patients with cholesteatoma (102 ears) who underwent retrograde tympanomastoidectomy and in whom bone chips/pat were applied as the sole materials during the procedure. In 79 ears, this was combined with ossiculoplasty in a single-stage procedure. In >71% of ears, the results of audiometric testing were monitored for more than 2 years. The results suggested there was a significant gain in hearing following surgery, with respect to the postoperative change in both air-conduction thresholds and air-bone gaps (P < 0.001). Linear regression analyses of pure-tone averages at different frequencies, before and after surgery, demonstrated that patients benefitted from a postoperative hearing gain at low and middle frequencies, but their hearing often deteriorated at frequencies of 8000Hz. As for the impact of the type of tympanoplasty on hearing outcomes, type III-interposition markedly increased hearing gain. The overall rate of postoperative adverse events was 8.8%. We conclude that reconstruction of the ear canal and mastoid via mastoid exclusion/obliteration using bone chips/pat can be considered as an alternative procedure following retrograde mastoidectomy. It gives excellent surgical results and has fewer postoperative adverse events.
Horng C.-T.,Kaohsiung Armed Force General Hospital |
Chiang N.-N.,Kaohsiung Veterans General Hospital Pingtung Branch |
Chen I.-L.,Tajen University |
Liang W.-Z.,Kaohsiung Veterans General Hospital |
And 4 more authors.
Journal of Receptors and Signal Transduction | Year: 2013
Clotrimazole is an antimycotic imidazole derivative that interferes with cellular Ca2+ homeostasis. This study examined the effect of clotrimazole on cytosolic Ca2+ concentrations ([Ca2+]i) and viability in HA59T human hepatoma cells. The Ca2+-sensitive fluorescent dye fura-2 was applied to measure [Ca2+]i. Clotrimazole induced [Ca2+]i rises in a concentration-dependent manner. The response was reduced by removing extracellular Ca2+. Clotrimazole-evoked Ca2+ entry was suppressed by store-operated channel inhibitors (nifedipine, econazole and SK&F96365) and protein kinase C modulators (GF109203X and phorbol, 12-myristate, 13-acetate). In Ca 2+-free medium, incubation with the endoplasmic reticulum Ca 2+ pump inhibitor 2,5-di-tert-butylhydroquinone abolished clotrimazole-induced [Ca2+]i rise. Inhibition of phospholipase C with U73122 abolished clotrimazole-induced [Ca2+]i rise. At 10-40 M, clotrimazole inhibited cell viability, which was not reversed by chelating cytosolic Ca2+. Clotrimazole at 10 and 30 M also induced apoptosis. Collectively, in HA59T cells, clotrimazole-induced [Ca2+]i rises by evoking phospholipase C-dependent Ca2+ release from the endoplasmic reticulum and Ca2+ entry via store-operated Ca2+ channels. Clotrimazole also caused apoptosis. © 2013 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Horng C.-T.,Kaohsiung Armed Force General Hospital |
Horng C.-T.,Chung Shan Medical University |
Horng C.-T.,Tajen University |
Huang J.-K.,Tajen University |
And 3 more authors.
Nutrients | Year: 2014
Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan. We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats. Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA). Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively). Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment. EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids. Trend analysis revealed that EPA dose-dependently scavenged DPPH free radicals. EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood. EPA dose-dependently decreased serum urea nitrogen and malondialdehyde levels after exercise. Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment. EPA could be a potential agent with an anti-fatigue pharmacological function. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
PubMed | Tajen University, National Taiwan Sport University and Kaohsiung Armed Force General Hospital
Type: Journal Article | Journal: Nutrients | Year: 2014
Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan. We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats. Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA). Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively). Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment. EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids. Trend analysis revealed that EPA dose-dependently scavenged DPPH free radicals. EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood. EPA dose-dependently decreased serum urea nitrogen and malondialdehyde levels after exercise. Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment. EPA could be a potential agent with an anti-fatigue pharmacological function.
PubMed | Tajen University, China Medical University at Taichung, Taichung Hospital and Kaohsiung Armed Force General Hospital
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2014
Chondrosarcoma, a primary malignant bone cancer, has potential for local invasion and distant metastasis, especially to the lungs. Patients diagnosed with it show poor prognosis. Paeonol (2-hydroxy-4-methoxyacetophenone), the main active compound of traditional Chinese remedy Paeonia lactiflora Pallas, exhibits anti-inflammatory and anti-tumor activity; whether paeonol regulates metastatic chondrosarcoma is largely unknown. Here, we find paeonol do not increase apoptosis. By contrast, at non-cytotoxic concentrations, paeonol suppresses migration and invasion of chondrosarcoma cells. We also demonstrate paeonol enhancing miR-141 expression and miR-141 inhibitor reversing paeonol-inhibited cell motility; paeonol also reduces protein kinase C (PKC)d and c-Src kinase activity. Since paeonol inhibits migration and invasion of human chondrosarcoma via up-regulation of miR-141 via PKCd and c-Src pathways, it thus might be a novel anti-metastasis agent for treatment of metastatic chondrosarcoma.