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Kashihara, Japan

Kansai University of Welfare science is a private university in Kashiwara, Osaka, Japan, established in 1997. Wikipedia.

Nagaki K.,Okayama University | Yamamoto M.,Kansai University of Welfare Sciences | Yamaji N.,Okayama University | Mukai Y.,Osaka Kyoiku University | Murata M.,Okayama University

Due to the ease with which chromosomes can be observed, the Allium species, and onion in particular, have been familiar materials employed in cytogenetic experiments in biology. In this study, centromeric histone H3 (CENH3)-coding cDNAs were identified in four Allium species (onion, welsh onion, garlic and garlic chives) and cloned. Anti-CENH3 antibody was then raised against a deduced amino acid sequence of CENH3 of welsh onion. The antibody recognized all CENH3 orthologs of the Allium species tested. Immunostaining with the antibody enabled clear visualization of chromosome behavior during mitosis in the species. Furthermore, three-dimensional (3D) observation of mitotic cell division was achieved by subjecting root sections to immunohistochemical techniques. The 3D dynamics of the cells and position of cell-cycle marker proteins (CENH3 and α-tubulin) were clearly revealed by immunohistochemical staining with the antibodies. The immunohistochemical analysis made it possible to establish an overview of the location of dividing cells in the root tissues. This breakthrough in technique, in addition to the two centromeric DNA sequences isolated from welsh onion by chromatin immuno-precipitation using the antibody, should lead to a better understanding of plant cell division. A phylogenetic analysis of Allium CENH3s together with the previously reported plant CENH3s showed two separate clades for monocot species tested. One clade was made from CENH3s of the Allium species with those of Poaceae species, and the other from CENH3s of a holocentric species (Luzula nivea). These data may imply functional differences of CENH3s between holocentric and monocentric species. Centromeric localization of DNA sequences isolated from welsh onion by chromatin immuno-precipitation (ChIP) using the antibody was confirmed by fluorescence in situ hybridization and ChIP-quantitative PCR. © 2012 Nagaki et al. Source

Niikawa Y.,Kansai University of Welfare Sciences
Proceedings - 2nd IIAI International Conference on Advanced Applied Informatics, IIAI-AAI 2013

In order that a specialist may perform 'learning of child and family welfare and reflection of practice', the development of reflective family social work is effective. And, the structure of reflective family social work educational program is reported by this research. Moreover, the reflective family social work practice education which utilized 'Family Support Power Education Program for Continuation and Revival of Independent Learning' was tried. © 2013 IEEE. Source

Shimizu H.,Kansai University of Welfare Sciences
Nihon rinsho. Japanese journal of clinical medicine

Angiotensin II is a potent stimulator of vascular smooth mustle cells that increases blood pressure, and angiotensin type 1 receptors have been identified on osteoblasts; thus, the renin-angiotensin system has been suggested to be involved in bone metabolism. The authors reported that angiotensin II significantly increased TRAP-positive multinuclear osteoclasts with the up-regulation of RANKL expression through extracellular kinase of osteoblast. These effects were abolished with co-treatment of angiotensin converting enzyme (ACE) inhibitors or angiotensin type 1 receptor blockers (ARB). Recently, RANK-RANKL-OPG system is proved to be involved in vascular calcification and RAAS also might be involved in the development of the disease. Thus, target therapy for RAAS might bring additional beneficial effects on bone metabolic diseases such as osteoporosis, rheumatoid arthritis and vascular calcification other than blood pressure lowering effect. Source

Yasuhiro N.,Kansai University of Welfare Sciences
Proceedings of the 2012 IIAI International Conference on Advanced Applied Informatics, IIAIAAI 2012

This study was reported the learning Web-site type database structure for the child and family welfare and reflection of practice. And, the reflective family social work practice education was tried. © 2012 IEEE. Source

Mizuno K.,RIKEN | Mizuno K.,Osaka City University | Tanaka M.,Osaka City University | Yamaguti K.,Osaka City University | And 4 more authors.
Behavioral and Brain Functions

Background: It is known that chronic fatigue is associated with sympathetic hyperactivity. However, the relationship between autonomic function and mental fatigue caused by a prolonged mental load in healthy humans is still unclear. Thus, in order to clarify the mechanisms underlying mental fatigue, we examined the association between mental fatigue and autonomic functions.Methods: The study group comprised 10 healthy participants. To induce mental fatigue, participants performed mental tasks, which consisted of the advanced trail making test, kana pick-out test and mirror drawing test, for 8 hr, corresponding to a normal work day. Autonomic functions were measured by accelerated plethysmography before and after the fatigue-inducing mental tasks. As a control, the same participants completed an 8-hr relaxation session 4 weeks before the fatigue session.Results: After the 8-hr relaxation session, low-frequency component power (LF), high-frequency component power (HF) and low-frequency component power/high-frequency component power ratio (LF/HF ratio) were not changed from baseline. In contrast, after the fatigue session, the HF and LF/HF ratio were significantly changed from baseline; specifically, the HF was lower and LF/HF ratio was higher as compared to those after the relaxation session.Conclusions: Sympathetic hyperactivity based on decreased parasympathetic activity is associated with mental fatigue induced by prolonged cognitive load. © 2011 Mizuno et al; licensee BioMed Central Ltd. Source

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