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Moukamnerd C.,Osaka University | Kino-Oka M.,Osaka University | Sugiyama M.,Osaka University | Kaneko Y.,Osaka University | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2010

To save cost and input energy for bioethanol production, a consolidated continuous solid-state fermentation system composed of a rotating drum reactor, a humidifier, and a condenser was developed. Biomass, saccharifying enzymes, yeast, and a minimum amount of water are introduced into the system. Ethanol produced by simultaneous saccharification and fermentation is continuously recovered as vapor from the headspace of the reactor, while the humidifier compensates for the water loss. From raw corn starch as a biomass model, 95∈±∈3, 226∈±∈9, 458∈±∈26, and 509∈±∈64 g∈l-1 of ethanol solutions were recovered continuously when the ethanol content in reactor was controlled at 10-20, 30-50, 50-70 and 75-85 g∈kg-mixture-1, respectively. The residue showed a lesser volume and higher solid content than that obtained by conventional liquid fermentation. The cost and energy for intensive waste water treatment are decreased, and the continuous fermentation enabled the sustainability of enzyme activity and yeast in the system. © 2010 Springer-Verlag. Source

Kansai Chemical Engineering Co. and Bio energy Corporation | Date: 2013-03-21

A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle.

A solution component recovery method, a solution component recovery apparatus, and an impregnation process/impregnation component recovery system for separating a first component from a second component. The separation between the first and second components is accomplished by reducing the pressure on a solution that contains the first component, which results in the solidification of the first component in the solution at a temperature that is equal to or higher than a predetermined solidification temperature. The second component, in which the first component is dissolved, is evaporated at a temperature range that is less than the predetermined solidification temperature. The evaporated second component is then recovered by a cooling step.

Bio Energy Corporation and Kansai Chemical Engineering Co. | Date: 2010-02-15

Provided is a lactic acid bacterium capable of homolactic fermentation using a pentose as a substrate, the lactic acid bacterium utilizing a pentose, and in which a phosphoketolase pathway is blocked and a pentose phosphate pathway is activated. Also provided is a method for producing lactic acid from a pentose using the lactic acid bacterium and a method for preparing the lactic acid bacterium.

Kobe University, Bio Energy Corporation and Kansai Chemical Engineering Co. | Date: 2010-12-10

The present invention provides a method for producing a cellulose degradable yeast, comprising the step of co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast sequence. According to the invention, a yeast having an improved cellulose degradation ability are provided.

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