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Yang H.,Guangdong Pharmaceutical University | Kang L.,Guangdong Pharmaceutical University | Hui Kai S.,Guangdong Pharmaceutical University | Sheng Quan L.,Guangdong Pharmaceutical University | Fei Jun X.,Kangmei Pharmaceutical Co.
Analytical Methods | Year: 2014

A method using molecularly imprinted solid-phase extraction combined with RP-HPLC-UV was developed for determination of liquiritin (LQR) in rat plasma after oral administration of Compound Liquorice Tablets (CLTs). To this end, molecularly imprinted polymers were prepared by in situ polymerization, using LQR as the template, acrylamide as the functional monomer, and ethylene glycol dimethacrylate as the crosslinker, whose solid-phase extraction properties for liquiritin were evaluated by HPLC-UV. The enrichment and extraction recovery were investigated in the study after the extraction procedure was optimized. The recovery was larger than 85.0% and the RSD was less than 10.0%. The calibration curve was expressed as y = 16 004x - 1.864 (r = 0.996) with a linear range of 0.007-1.683 μg mL-1. Besides, the Tmax was 0.5 h for liquiritin and the Cmax was 0.8 μg mL-1 after oral administration of compound liquorice tablets. In addition, the pharmacokinetic parameters were calculated using a two-compartment model. The results indicated that the monolithic polymer pipette presented a good extraction efficiency for liquiritin. At the same time, the results indicated that the index components could basically reveal the pharmacokinetic behavior. This study was aimed to explore an approach that could be applied to promulgate the pharmacokinetics and provide more information for clinical applications. © 2014 The Royal Society of Chemistry. Source


Li Y.,CAS Changchun Institute of Applied Chemistry | Li Y.,Chinese Academy of Sciences | Yang X.,CAS Changchun Institute of Applied Chemistry | Wang Y.,Chinese Academy of Sciences | Wang Y.,Kangmei Pharmaceutical Co.
Analytical Letters | Year: 2012

Polyoxometalates (POMs) possess an excellent property for electrochemical catalysis. A new hybrid complex containing polyoxometalate anions and layered double hydroxide (LDH) was prepared by assembly of Mg-Al LDH precursors in nitrate form with the 12-molybdophosphoric acid anions [PMo12O40]3-(PMo12). The properties of the product complexes were characterized by the methods of field-emission scanning electron microscopy (FE-SEM), spectroscopic techniques (UV-vis, FT-IR), elemental analysis (EDS), and electrochemistry. The electrochemical behaviors of the LDH/PMo12 film were investigated by cyclic voltammetry. The result indicated that the LDH/PMo12 nanocomposite films modified electrode exhibited a diffusion-controlled voltammetric feature. The films were able to catalyze the reduction of halate ions (ClO3 -,BrO3 - and IO3 -). Therefore, the prepared nanocomposite films modified electrode had potential application in the electroanalytical field. © 2012 Copyright Taylor and Francis Group, LLC. Source


Zhang Z.-X.,Hanshan Normal University | Fu Q.,Peking University | Zheng K.Y.-Z.,Hanshan Normal University | Zheng K.Y.-Z.,Sun Yat Sen University | Zheng K.Y.-Z.,Kangmei Pharmaceutical Co.
Journal of Asian Natural Products Research | Year: 2013

Three new sulfated triterpene glycosides, asprellanosides C-E (1-3), were isolated from the roots of Ilex asprella. Their structures were elucidated as 3β-[(2-O-sulfo-β-D-xylopyranosyl)oxy]urs-12,19(29)-diene-28-oic acid 28-β-D-glucopyranoside (1), 3β-[(2-O-sulfo-β-D-xylopyranosyl)oxy] urs-12,19-diene-28-oic acid 28-β-D-glucopyranoside (2), and 3β-[(2-O-sulfo-β-D-xylopyranosyl)oxy]urs-12,19-diene-28-oic acid (3) on the basis of the spectral and chemical methods. © 2013 Taylor & Francis. Source


Li Y.,Chinese Academy of Sciences | Li Y.,CAS Changchun Institute of Applied Chemistry | Yang X.,CAS Changchun Institute of Applied Chemistry | Yang F.,CAS Changchun Institute of Applied Chemistry | And 4 more authors.
Electrochimica Acta | Year: 2012

Phosphomolybdic acid anion ([PMo 12O 40] 3-) was used for the immobilization of ruthenium(II) tris(bipyridine) (Ru(bpy) 3 2+) on an electrode surface to yield a sensitive solid-state electrogenerated chemiluminescence (ECL) sensor. [PMo 12O 40] 3- anion in the prepared sensor had catalytic ability to the NADH oxidation. The ECL signal of the Ru(bpy) 3 2+/[PMo 12O 40] 3- film was about 3-fold enhancement than that for the Ru(bpy) 3 2+/Nafion film to NADH determination. The resulting ECL sensor exhibited a wide linear range from 2.5 × 10 -7 to 5.0 × 10 -3 M (R = 0.99) with the detection limit of 1.67 × 10 -8 M (S/N = 3). In addition, it had good reproducibility and excellent long-term stability, and the relative average deviation was 0.77% of ECL intensity-time curve under continuous potential scanning for 21 cycles; after being used in two weeks, the sensor was able to keep over 90% activity toward 25 μM NADH. Fabrication of the ECL sensor by this method is simple and easy. Such superior properties will promote the application of polyoxometalates in fabricating sensors for using in electroanalytical and biochemical analysis. © 2012 Elsevier Ltd. All rights reserved. Source


Jiang Y.H.,Kangmei Pharmaceutical Co.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To determine the 6 residues including organophosphorus, organochlorinated and pyrethroid pesticides simultaneously in crude yam slices, yam slices stir-fried with bran and sulfur fumigation yam. The compounds were ready for GC-MS analysis after being extracted with acetonitrile and purified. Determinations were performed with selected ion monitoring mode and external standard method was adopted in the quantificaticon. The contents of 6 kinds pesticides residue had good linear correlation with the peak area in the rang of 0.01-0.5 microg/mL. The average recoveries of 6 kinds pesticides residue were 87.5%-95.5%. The method detection limits ranged from 0.002-0.005 microg/g. Organochlorine and pyrethroid pesticides were detected in the three samples except the parathion residue. Stir-fried with bran and sulphur fumigation had nearly no effects on the pesticides residue in yam. The method is sensitive and accurate and can be used in determination of multiresidual pesticides residue in yam. Source

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