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Isesaki, Japan

Nukui S.,Niigata University | Kitamura S.,Niigata University | Kitamura S.,Kaneko Seeds Co. | Hioki T.,Niigata University | And 6 more authors.
Breeding Science | Year: 2011

Fertile plants undergoing male gametogenesis can be treated with nitrous oxide (N 2O) gas to obtain 2n male gametes. N 2O treatment is also expected to restore the fertility of interspecific hybrids through meiotic restitution or mitotic amphidiploidization. However, this technique has few applications to date, and it is unknown how N 2O treatment restores fertility in sterile hybrids. To establish optimal N 2O treatment conditions and determine its cytological mechanism of action, we treated various sized floral buds with N 2O gas at different anther developmental stages from fertile and sterile hybrid lilies. N 2O treatment using the optimal 1- 4 mm floral buds induced mitotic polyploidization of male archesporial cells to produce 2n pollen in fertile hybrid lilies. In sterile hybrid lilies, N 2O treatment doubled the chromosome number in male archesporial cells followed by homologous chromosome pairing and normal meiosis in pollen mother cells (PMC), resulting in restoration of pollen fertility. Backcrossing the resultant fertile pollen to Lilium × formolongi produced many triploid BC1 plants. Thus N 2O treatment at the archesporial cell proliferating stage effectively overcame pollen sterility in hybrid lilies, resulting in fertile, 2n pollen grains that could produce progeny. The procedure presented here will promote interspecific or interploidy hybridization of lilies.

Tomioka K.,Japan National Institute of Agrobiological Science | Sato T.,Japan National Institute of Agrobiological Science | Moriwaki J.,Horticultural Research Institute | Terasawa Y.,Kaneko Seeds Co. | Koganezawa H.,Kaneko Seeds Co.
Journal of General Plant Pathology | Year: 2012

Severe spotting and blighting of leaves were found on bacopa (Sutera cordata), a scrophulariaceous ornamental, in greenhouses in Gunma Prefecture, Japan, from January through February 2007. After we isolated and identified the causal fungus as Colletotrichum destructivum and inoculated host plants with the isolate to confirm pathogenicity, we named this new disease anthracnose of bacopa. © 2012 The Phytopathological Society of Japan and Springer.

Oikawa E.,Tohoku University | Takuno S.,Tohoku University | Takuno S.,University of California at Irvine | Izumita A.,Sakata Seed Corporation | And 4 more authors.
Molecular Breeding | Year: 2011

In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i. e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds. © 2010 Springer Science+Business Media B.V.

Shirasawa S.,Tohoku University | Kifuji Y.,Tohoku University | Kifuji Y.,Kaneko Seeds Co. | Komiya R.,Tohoku University | And 4 more authors.
Molecular Breeding | Year: 2013

Stable genic male sterility (GMS), which is not influenced by environmental factors, has not been used for F1 hybrid seed production because male-sterile inbred lines cannot be developed and male-sterile plants must be selected from segregating populations every time. However, the stability of male sterility may provide a reliable system for F1 seed production without contamination of selfed seeds. A genic male-sterile mutant in rice (Oryza sativa L.), C204, which was selected from progeny of the cultivar 'Koshihikari' irradiated by gamma rays, has shorter and whiter anthers than those of 'Koshihikari' and has no pollen grains. Segregation analysis of C204 suggested the male sterility of this mutant to be controlled by a recessive allele of a single gene. Linkage analysis of a mutated gene responsible for the male sterility revealed the gene to be in a region of ca. 75 kb on the long arm of chromosome 9. The nine genes predicted in the 75-kb region were sequenced, and compared with the published Nipponbare genome sequences. A single-base deletion was found in the first exon of a C204 allele of Os09g0493500, which encodes an NAD-dependent epimerase/dehydratase family protein, resulting in a frameshift causing a premature stop codon. A dot-blot single nucleotide polymorphism marker for detection of the single-base deletion in Os09g0493500 was developed. We herein propose an F1 hybrid seed production system using stable GMS with a simple selection method of GMS plants. © 2013 Springer Science+Business Media Dordrecht.

Kaneko Seeds Co. | Date: 2011-11-22

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