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Kanazawa-shi, Japan

Munesue S.,Kanazawa University | Yamamoto Y.,Kanazawa University | Urushihara R.,Kanazawa University | Inomata K.,Tohoku Pharmaceutical University | And 6 more authors.
Food and Function | Year: 2013

Advanced glycation end-products (AGE) have been implicated in aging and the pathogenesis of diabetic complications, inflammation, Alzheimer's disease, and cancer. AGE engage the cell surface receptor for AGE (RAGE), which in turn elicits intracellular signaling, leading to activation of NF-κB to cause deterioration of tissue homeostasis. AGE are not only formed within our bodies but are also derived from foods, endowing them with flavor. In the present study, we assessed the agonistic/antagonistic effects of food-derived AGE on RAGE signaling in a reporter assay system and found that low-molecular weight AGE can antagonize the action of AGE-BSA. Foods tested were Japanese soy sauce, coffee, cola, and red wine, all of which showed fluorescence characteristics of AGE. Soy sauce and coffee contained Nε-carboxymethyl-lysine (CML). Soy sauce, coffee, and red wine inhibited the RAGE ligand-induced activation of NF-κB, whereas cola had no effect on the ligand induction of NF-κB. The liquids were then fractionated into high-molecular weight (HMW) fractions and low-molecular weight (LMW) fractions. Soy sauce-, coffee-, and red wine-derived LMW fractions consistently inhibited the RAGE ligand induction of NF-κB, whereas the HMW fractions of these foods activated RAGE signaling. Using the LMW fraction of soy sauce as a model food-derived RAGE antagonist, we performed a plate-binding assay and found that the soy sauce LMW fractions competitively inhibited AGE-RAGE association. Further, this fraction significantly reduced AGE-dependent monocyte chemoattractant protein-1 (MCP-1) secretion from murine peritoneal macrophages. The LMF from soy sauce suppressed the AGE-induced RAGE trafficking to lipid rafts. These results indicate that small components in some, if not all, foods antagonize RAGE signaling and could exhibit beneficial effects on RAGE-related diseases. © 2013 The Royal Society of Chemistry.

Oguntoyinbo B.,Kanazawa Institute of Technology | Hirama J.,Kanazawa Institute of Technology | Yanagibashi H.,Kanazawa Technical College | Matsui Y.,Kanazawa Gakuin College | And 5 more authors.
Environmental Control in Biology | Year: 2015

This paper describes the principles of automating the Maitake (Grifola frondosa Dicks. Fr.S. F. Gray) mushroom's cultivation process using the developed speaking mushroom approach (SMA) system. The system measures the bioelectric potential signal from the mushrooms and uses it as a control parameter for the lighting conditions. The purpose of the SMA system is to allow optimal control over the cultivation environment in order to improve the running cost and production yields within mushroom factories. It uses sensors and actuators to maintain the optimal temperature and humidity, and uses the known inherent mushroom bioelectric potential to control the lights. The results from the SMA system in this experiment showed a clear bioelectric potential present in the Maitake fruit body. These bioelectric potential signals reflect the internal rhythm of the mushroom as well as external stimulations. Analysing the measured signals the SMA system demonstrated that it will be possible to save energy and time during mushroom cultivation.

Oguntoyinbo B.,Kanazawa Institute of Technology | Ozawa T.,Kanazawa Institute of Technology | Kawabata K.,Kanazawa Institute of Technology | Hirama J.,Kanazawa Institute of Technology | And 6 more authors.
IFAC Proceedings Volumes (IFAC-PapersOnline) | Year: 2013

This paper defines the principles of automating the mushroom cultivation process by using their bioelectric potential feedback as a control parameter. Previous studies within this research group examined different environmental stimulations with respect to mushroom bioelectric potential feedback characteristics. This SMA (Speaking Mushroom Approach) system proposes the use of these identified bioelectric feedback characteristics as a method to control the environmental variables. It is theorized that through this system, optimal growth conditions can be realized in a repeatable and reliable manner.

Han D.,Kanazawa University | Yamamoto Y.,Kanazawa University | Munesue S.,Kanazawa University | Motoyoshi S.,Kanazawa University | And 6 more authors.
Genes to Cells | Year: 2013

Glucolipotoxicity, which is exerted by free fatty acids (FFA) and prolonged hyperglycemia, is implicated in pancreatic β-cell failure in diabetes. Pattern recognition receptors such as receptor for advanced glycation end products (RAGE) and toll-like receptors 2 and 4 could mediate danger signals in β-cells. We examined whether RAGE contributes to β-cell failure in a type 2 diabetes mouse model. Pancreatic islets were isolated from ob/ob, db/db, diet-induced obesity (DIO), RAGE-null (RAGE-/-), and RAGE+/+ wild-type (WT) control mice and dispersed into single cells for flow cytometry. RAGE expression was detected in insulin-positive β-cells of ob/ob and db/db mice, but not of WT, DIO, or RAGE-/- mice: thus, inadequate leptin receptor signaling and RAGE expression may be linked. Compared with RAGE+/+ db/db mice, RAGE-/- db/db mice showed higher β-cell number and mass with less apoptosis as well as glucose tolerance with higher insulin secretion without any differences in serum levels of FFA and adiponectin. Palmitate or oleate pretreatment combined with a leptin antagonist induced RAGE expression, AGE-elicited apoptosis, and impaired glucose-stimulated insulin secretion by advanced glycation end products (AGE) in MIN6 cells. FFA elevation with concomitant AGE formation during prolonged hyperglycemia could cause β-cell damage through insufficient leptin action and subsequent RAGE induction in type 2 diabetes. © 2013 The Authors Genes to Cells © 2013 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.

Shibata A.,Kanazawa University | Hitomi Y.,Kanazawa University | Kambayashi Y.,Kanazawa University | Hibino Y.,Kanazawa University | And 7 more authors.
Research in Autism Spectrum Disorders | Year: 2013

Although autism is now recognized as being very common (Buie et al.; 2010) and as developing due to not only genetic but also environmental factors, there is insufficient epidemiological evidence on the relationship between autism and allergy. In this study, therefore, we attempted to clarify the association of environmental factors with autism and allergy using a population-based epidemiological study and to propose a newly developed screening method with improved validity by determining the relationship between ASD (autism spectrum disorders) subscales. We examined the association among autistic score from the Autism Screening Questionnaire (ASQ Japanese version), living environment factors, and allergic disease in 1407 children aged 3-5 years old. We recognized that children with higher ASD score have significantly higher prevalence of nasal allergy and associated with significantly factors of boy and first in terms of birth order and maternal smoking. In addition, nasal allergy were observed to be associated with "reciprocal social interaction", "language and communication", and "repetitive and stereotyped patterns of behaviors" as subscales of ASD. These results suggest that autism in children were closely related to allergy and environmental factors. Investigation of allergy symptoms in addition to subscale of ASD seem to enable more efficient screening of autism tendency at an earlier stage. © 2012 Elsevier Ltd. All rights reserved.

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