Kanazawa Gakuin College

Kanazawa-shi, Japan

Kanazawa Gakuin College

Kanazawa-shi, Japan

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Awatsuhara R.,Kanazawa Gakuin College | Harada K.,National Fisheries University | Maeda T.,National Fisheries University | Nomura T.,Kanazawa Gakuin College | Nagao K.,Tokyo Kasei University
Molecular Medicine Reports | Year: 2010

Buckwheat flour is well known for its highly anti-oxidative ingredient, rutin. We have undertaken to examine alterations in the characteristics of rutin treated with various proteins. In this study, the radical scavenging activities of a rutin-ovalbumin complex were examined. Dissolved rutin hydrate and ovalbumin were combined and boiled in water for 10 min. In the resulting rutin-ovalbumin complex, a new high molecular weight peak was detected using gel permeation chromatography analysis, and an existing high molecular weight area of ovalbumin was observed to be increased by the addition of rutin. This suggested that ovalbumin molecules produce a complex through their interaction with rutin. Alkaline luminol chemiluminescence and electron spin resonance analysis revealed the formation of a rutin-ovalbumin complex that markedly enhanced the peroxyl, but not the hydroxyl, radical scavenging activity of rutin. Rutin also demonstrated antioxidative activity against hydroxyl radicals in a DNA protection assay. We therefore conclude that, compared with ovalbumin or rutin alone, the rutin-ovalbumin complex has improved antioxidative activities in the form of enhanced peroxyl radical scavenging activity and DNA protection from apurinic/apyrimidinic site formation caused by hydroxyl radicals.


Munesue S.,Kanazawa University | Yamamoto Y.,Kanazawa University | Urushihara R.,Kanazawa University | Inomata K.,Tohoku Pharmaceutical University | And 6 more authors.
Food and Function | Year: 2013

Advanced glycation end-products (AGE) have been implicated in aging and the pathogenesis of diabetic complications, inflammation, Alzheimer's disease, and cancer. AGE engage the cell surface receptor for AGE (RAGE), which in turn elicits intracellular signaling, leading to activation of NF-κB to cause deterioration of tissue homeostasis. AGE are not only formed within our bodies but are also derived from foods, endowing them with flavor. In the present study, we assessed the agonistic/antagonistic effects of food-derived AGE on RAGE signaling in a reporter assay system and found that low-molecular weight AGE can antagonize the action of AGE-BSA. Foods tested were Japanese soy sauce, coffee, cola, and red wine, all of which showed fluorescence characteristics of AGE. Soy sauce and coffee contained Nε-carboxymethyl-lysine (CML). Soy sauce, coffee, and red wine inhibited the RAGE ligand-induced activation of NF-κB, whereas cola had no effect on the ligand induction of NF-κB. The liquids were then fractionated into high-molecular weight (HMW) fractions and low-molecular weight (LMW) fractions. Soy sauce-, coffee-, and red wine-derived LMW fractions consistently inhibited the RAGE ligand induction of NF-κB, whereas the HMW fractions of these foods activated RAGE signaling. Using the LMW fraction of soy sauce as a model food-derived RAGE antagonist, we performed a plate-binding assay and found that the soy sauce LMW fractions competitively inhibited AGE-RAGE association. Further, this fraction significantly reduced AGE-dependent monocyte chemoattractant protein-1 (MCP-1) secretion from murine peritoneal macrophages. The LMF from soy sauce suppressed the AGE-induced RAGE trafficking to lipid rafts. These results indicate that small components in some, if not all, foods antagonize RAGE signaling and could exhibit beneficial effects on RAGE-related diseases. © 2013 The Royal Society of Chemistry.


Oguntoyinbo B.,Kanazawa Institute of Technology | Hirama J.,Kanazawa Institute of Technology | Yanagibashi H.,Kanazawa Technical College | Matsui Y.,Kanazawa Gakuin College | And 5 more authors.
Environmental Control in Biology | Year: 2015

This paper describes the principles of automating the Maitake (Grifola frondosa Dicks. Fr.S. F. Gray) mushroom's cultivation process using the developed speaking mushroom approach (SMA) system. The system measures the bioelectric potential signal from the mushrooms and uses it as a control parameter for the lighting conditions. The purpose of the SMA system is to allow optimal control over the cultivation environment in order to improve the running cost and production yields within mushroom factories. It uses sensors and actuators to maintain the optimal temperature and humidity, and uses the known inherent mushroom bioelectric potential to control the lights. The results from the SMA system in this experiment showed a clear bioelectric potential present in the Maitake fruit body. These bioelectric potential signals reflect the internal rhythm of the mushroom as well as external stimulations. Analysing the measured signals the SMA system demonstrated that it will be possible to save energy and time during mushroom cultivation.


Oguntoyinbo B.,Kanazawa Institute of Technology | Ozawa T.,Kanazawa Institute of Technology | Kawabata K.,Kanazawa Institute of Technology | Hirama J.,Kanazawa Institute of Technology | And 6 more authors.
IFAC Proceedings Volumes (IFAC-PapersOnline) | Year: 2013

This paper defines the principles of automating the mushroom cultivation process by using their bioelectric potential feedback as a control parameter. Previous studies within this research group examined different environmental stimulations with respect to mushroom bioelectric potential feedback characteristics. This SMA (Speaking Mushroom Approach) system proposes the use of these identified bioelectric feedback characteristics as a method to control the environmental variables. It is theorized that through this system, optimal growth conditions can be realized in a repeatable and reliable manner.


Yokoyama H.,University of Shizuoka | Mizutani R.,Tokai University | Satow Y.,University of Tokyo | Sato K.,Hokkaido University | And 3 more authors.
Acta Crystallographica Section D: Biological Crystallography | Year: 2012

Pyrimidine (6-4) pyrimidone DNA photoproducts produced by ultraviolet light are highly mutagenic and carcinogenic. The crystal structure of the dTT(6-4)TT photoproduct in complex with the Fab fragment of the antibody 64M-2 that is specific for (6-4) photoproducts was determined at 2.4 Å resolution. The dT(6 - 4)T segment is fully accommodated in the concave binding pocket of the Fab, as observed in the complex of dT(6 - 4)T with the Fab. The pyrimidine and pyrimidone bases of the dT(6 - 4)T segment are positioned nearly perpendicularly to each other. The thymidine segments flanking both ends extend away from the dT(6-4)T segment. The 5́-side thymine base is parallel to the side chain of Tyr100iH of the antibody heavy chain and is also involved in electrostatic interactions with Asn30L, Tyr32L and Lys50L of the antibody light chain. The 5́-side and 3́-side phosphate groups exhibit electrostatic inter-actions with Asn28L and Ser58H, respectively. These inter-actions with the flanking nucleotides explain why longer oligonucleotides containing dT(6-4)T segments in the centre show higher antibody-binding affinities than the dT(6 - 4)T ligand. © 2012 International Union of Crystallography Printed in Singapore - all rights reserved.


Nishino K.,Kanazawa Gakuin College | Nomura T.,Kanazawa Gakuin College | Takeshita Y.,Kanazawa University | Komatsu T.,Doshisha Women's College of Liberal Arts | Takamura T.,Kanazawa University
Journal of the Japan Diabetes Society | Year: 2013

The effects of split meal serving were examined in middle-aged overweight males working at night at the same company. All of the subjects consumed a large dinner late at night without breakfast; therefore, they had metabolic problems, including visceral fat accumulation, fatty liver, high serum TG levels and low adi-ponectin levels. Their dinner was divided into two meals with the same total energy count: a light supper in the evening (around 7 pm) and a smaller dinner at night. Eating breakfast, consuming less sweets and engaging in light physical exercise were also recommended. The subjects reduced both their body mass and BMI. The serum TG levels and fatty liver significantly improved, and the serum adiponectin levels increased after eight weeks of intervention. Therefore, the use of split dinner servings is suggested to be effective for controlling obesity and related syndromes.


Shibata A.,Kanazawa University | Hitomi Y.,Kanazawa University | Kambayashi Y.,Kanazawa University | Hibino Y.,Kanazawa University | And 7 more authors.
Research in Autism Spectrum Disorders | Year: 2013

Although autism is now recognized as being very common (Buie et al.; 2010) and as developing due to not only genetic but also environmental factors, there is insufficient epidemiological evidence on the relationship between autism and allergy. In this study, therefore, we attempted to clarify the association of environmental factors with autism and allergy using a population-based epidemiological study and to propose a newly developed screening method with improved validity by determining the relationship between ASD (autism spectrum disorders) subscales. We examined the association among autistic score from the Autism Screening Questionnaire (ASQ Japanese version), living environment factors, and allergic disease in 1407 children aged 3-5 years old. We recognized that children with higher ASD score have significantly higher prevalence of nasal allergy and associated with significantly factors of boy and first in terms of birth order and maternal smoking. In addition, nasal allergy were observed to be associated with "reciprocal social interaction", "language and communication", and "repetitive and stereotyped patterns of behaviors" as subscales of ASD. These results suggest that autism in children were closely related to allergy and environmental factors. Investigation of allergy symptoms in addition to subscale of ASD seem to enable more efficient screening of autism tendency at an earlier stage. © 2012 Elsevier Ltd. All rights reserved.


Han D.,Kanazawa University | Yamamoto Y.,Kanazawa University | Munesue S.,Kanazawa University | Motoyoshi S.,Kanazawa University | And 6 more authors.
Genes to Cells | Year: 2013

Glucolipotoxicity, which is exerted by free fatty acids (FFA) and prolonged hyperglycemia, is implicated in pancreatic β-cell failure in diabetes. Pattern recognition receptors such as receptor for advanced glycation end products (RAGE) and toll-like receptors 2 and 4 could mediate danger signals in β-cells. We examined whether RAGE contributes to β-cell failure in a type 2 diabetes mouse model. Pancreatic islets were isolated from ob/ob, db/db, diet-induced obesity (DIO), RAGE-null (RAGE-/-), and RAGE+/+ wild-type (WT) control mice and dispersed into single cells for flow cytometry. RAGE expression was detected in insulin-positive β-cells of ob/ob and db/db mice, but not of WT, DIO, or RAGE-/- mice: thus, inadequate leptin receptor signaling and RAGE expression may be linked. Compared with RAGE+/+ db/db mice, RAGE-/- db/db mice showed higher β-cell number and mass with less apoptosis as well as glucose tolerance with higher insulin secretion without any differences in serum levels of FFA and adiponectin. Palmitate or oleate pretreatment combined with a leptin antagonist induced RAGE expression, AGE-elicited apoptosis, and impaired glucose-stimulated insulin secretion by advanced glycation end products (AGE) in MIN6 cells. FFA elevation with concomitant AGE formation during prolonged hyperglycemia could cause β-cell damage through insufficient leptin action and subsequent RAGE induction in type 2 diabetes. © 2013 The Authors Genes to Cells © 2013 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.

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