Kanak Manjari Institute of Pharmaceutical science

Rourkela, India

Kanak Manjari Institute of Pharmaceutical science

Rourkela, India
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Saha P.,Kanak Manjari Institute of Pharmaceutical science
Tropical Journal of Pharmaceutical Research | Year: 2010

Purpose: To develop ampicillin trihydrate-loaded chitosan nanoparticles by modified ionic gelation method and evaluate their antimicrobial activity. Methods: Ampicillin trihydrate-loaded chitosan nanoparticles were prepared by ionic gelation method with the aid of sonication. Parameters such as the zeta potential, polydispersity, particle size, entrapment efficiency and in vitro drug release of the nanoparticles were assessed for optimization. The antibacterial properties of the nanoparticle formulation were evaluated and compared with that of a commercial formulation (reference). Results: Scanning electron microscopy revealed that the nanoparticles were in the nanosize range but irregular in shape. Concentrations of 0.35 %w/v of chitosan and 0.40 %w/v sodium tripolyphosphate (TPP) and a sonication time of 20 min constituted the optimum conditions for the preparation of the nanoparticles. In vitro release data showed an initial burst followed by slow sustained drug release. The nanoparticles demonstrated superior antimicrobial activity to plain nanoparticles and the reference, due probably to the synergistic effect of chitosan and ampicillin trihydrate. Conclusion: Modified ionic gelation method can be utilized for the development of chitosan nanoparticles of ampicillin trihydrate. Polymer and crosslinking agent concentrations and sonication time are rate-limiting factors for the development of the optimized formulation. The chitosan nanoparticles developed would be capable of sustained delivery of ampicillin trihydrate. © Pharmacotherapy Group.


Mukherjee A.,Bhabha Atomic Research Center | Sahoo S.,Kanak Manjari Institute of Pharmaceutical science | Sarma H.D.,Bhabha Atomic Research Center | Chakraborti C.K.,Kanak Manjari Institute of Pharmaceutical science | Samuel G.,Bhabha Atomic Research Center
Applied Radiation and Isotopes | Year: 2014

Preparation of three mucoadhesive formulations was optimized and pharmaceutically evaluated. Ofloxacin was radiolabeled with 99mTc and radiolabeled complex was characterized by HPLC. 99mTc-Ofloxacin was added as a tracer to the formulations namely Oflox C934, Oflox C940 and Oflox HPMC and the formulations were fed orally to rats. Imaging studies were carried out to assess the prolonged gastric retention of the formulations. 99mTc-Ofloxacin served as a good tracer for studying the pharmacokinetics of three controlled release mucoadhesive dosage forms by gamma scintigraphy studies. © 2014 Elsevier Ltd.


Sahoo S.,Kanak Manjari Institute of Pharmaceutical science | Chakraborti C.K.,Kanak Manjari Institute of Pharmaceutical science | Behera P.K.,Sambalpur University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Very few formulations are available, at present, from which the drug is absorbed uniformly, so that safe and effective blood level of Ciprofloxacin could be maintained for a prolonged period. Considering this limitation, a controlled release mucoadhesive suspension of Ciprofloxacin with Carbopol polymer (Carbopol940) has been prepared following a novel method of ultrasonication. The chemical interaction between Ciprofloxacin and polymer in formulation has been studied by FTIR and Raman Spectroscopy. From the spectral interpretation, it has been found that in formulation, the carboxylic groups of Ciprofloxacin and hydroxyl groups of Carbopol940 undergo chemical interaction, leading to esterification and hydrogen bonding. The formation of micellies due to esterification and hydrogen bonding causes more drug entrapment and a stable formulation. Due to that the formulation of Ciprofloxacin gives better controlled release and mucoadhesive action in the gastrointestinal tract. Hence, Carbopol940 could be considered as an effective carrier for Ciprofloxacin.


Sahoo S.,Kanak Manjari Institute of Pharmaceutical science | Chakraborti C.K.,Kanak Manjari Institute of Pharmaceutical science | Mishra S.C.,National Institute of Technology Rourkela
African Journal of Microbiology Research | Year: 2011

Considering various effects of mucoadhesive suspension containing Ciprofloxacin, it was found that newly designed mucoadhesive formulation was better than its conventional immediate release preparation. Due to that their in vitro antibacterial activity was also studied and compared. It was found that the newly designed mucoadhesive formulation showed much larger zones of inhibition against all the strains used in the study than the conventional immediate release preparation. In addition, this novel formulation and even the standard discs of Ciprofloxacin produced more or less similar zones of inhibition. However, the conventional immediate release preparation was much inferior to the standard discs as far as the zones of inhibition were concerned. ©2011 Academic Journals.


Sahoo S.,Kanak Manjari Institute of Pharmaceutical science | Chakraborti C.K.,Kanak Manjari Institute of Pharmaceutical science | Mishra S.C.,National Institute of Technology Rourkela
International Journal of Pharmacy and Technology | Year: 2011

Aims: Ofloxacin, an antibacterial agent, is having low solubility in aqueous solution. Moreover, the drug possesses short half-life, so frequent dosing is required. To overcome these difficulties, many researchers have prepared different formulations of Ofloxacin. But till now very few formulations are available from which the drug is absorbed uniformly, so that safe and effective blood level of Ofloxacin can be maintained for a prolonged period. To fulfill this requirement, in the present study, a controlled release drug delivery system has been designed and chemical interaction between Ofloxacin and polymer in formulation has been studied by FTIR and Raman Spectroscopy. Methods: Ultrasonication method was used for preparation of mucoadhesive Ofloxacin formulation, taking HPMC polymer with drug to polymer weight ratio 1:5. FTIR (400 cm -1 to 4000 cm -1 region) and Raman (140 to 2400 cm -1 region) Spectroscopic studies were carried out and spectra were used for interpretation. Results: From the spectral interpretation, it has been found that in formulation, the carboxylic groups of Ofloxacin and hydroxyl groups of HPMC undergo chemical interaction leading to esterification and hydrogen bonding (both intermolecular and polymeric). Conclusion: The formation of micellies due to esterification and hydrogen bonding causes more drug entrapment and formation of a stable formulation. As a result, the mucoadhesive formulation of Ofloxacin gives better controlled release and mucoadhesive action in the gastrointestinal tract. Hence, HPMC can be considered as an effective carrier of Ofloxacin.


Sahoo S.,Kanak Manjari Institute of Pharmaceutical science | Chakraborti C.K.,Kanak Manjari Institute of Pharmaceutical science | Behera P.K.,Sambalpur University
International Journal of Pharma and Bio Sciences | Year: 2012

Mucoadhesive polymeric (HPMC) suspension of Ciprofloxacin was prepared by ultrasonication and optimised with the aim to develop an oral controlled release gastroretentive dosage form. The qualitative analysis of the formulation was performed by Fourier Transform Infrared Spectroscopy (FTIR), Raman Spectroscopy, X-ray powder diffraction (XRD) and Scanning electron microscopy (SEM). FTIR (400 cm-1 to 4000 cm-1 region) and Raman (140 to 2400 cm-1 region) spectra were used for interpretation. XRD data of pure drug, polymer and the formulation were obtained using a powder diffractometer. The dispersion of particle was observed using SEM techniques. The results from FTIR and Raman Spectroscopic analyses suggested that in formulation, the carboxylic groups of Ciprofloxacin and hydroxyl groups of HPMC undergo chemical interaction leading to esterification and hydrogen bonding. The XRD data suggested that the retention of crystalline nature of Ciprofloxacin in the formulation could lead to increase in stability and drug loading; decrease in solubility; and delay in release of the drug from polymeric suspension with better bioavailability and penetration capacity. The SEM image analysis provided supporting evidences for homogeneous, uniformly dispersed, stable, controlled release of Ciprofloxacin suspension which would be pharmaceutically acceptable.


Chakraborti C.,Kanak Manjari Institute of Pharmaceutical science
Indian Journal of Pharmacology | Year: 2011

Presence of vitamin D receptors in noncalcemic tissues and subsequent identification of its involvement in growth factor(s)-mediated cellular function suggested its probable beneficial role in genesis, progression and survival of cancerous growths. Data collected from both in vitro and in vivo studies are highly optimistic regarding its potential in prevention and regression of colorectal, prostate and breast cancers. The vitamin has been found to interfere with the transduction pathways of various growth factor(s)-activated receptors (receptor tyrosine kinases) thereby modulating transcription and alteration of genomic functions resulting in inhibition of cell proliferation and angiogenesis and facilitation of cell differentiation and apoptosis. It also increases the level of an endogenous protein - cystatin D, which possesses antitumor and antimetastatic property, by facilitation of the expression of the gene coding for it. Though not as a primary anticancer agent, this vitamin may be used for the prevention of cancer and included as an adjuvant in combination chemotherapy for the treatment of cancer.


Sahoo S.,Kanak Manjari Institute of Pharmaceutical science | Chakraborti C.K.,Kanak Manjari Institute of Pharmaceutical science | Naik S.,Kanak Manjari Institute of Pharmaceutical science | Mishra S.C.,National Institute of Technology Rourkela | Nanda U.N.,Seemanta Institute of Pharmaceutical science
Tropical Journal of Pharmaceutical Research | Year: 2011

Purpose: To evaluate physicochemical changes in ciprofloxacin following incorporation in Carbopol polymeric composites. Methods: The ciprofloxacin and Carbopol were mixed in water in a drug:polymer ratio of 1:5 (w/w) and homogenized to produce uniform composites. X-ray powder diffraction analysis of the pure ciprofloxacin and the Carbopol polymeric composites of the drug were obtained using a powder diffractometer. Spectra for the materials were also generated by Fourier transform infrared (FTIR) spectroscope interfaced with an infrared (IR) microscope operated in reflectance mode. Results: Based on the Hanawalt system, three prominent x-ray diffractogram (XRD) peaks of the pure ciprofloxacin and the drug in the polymeric composites exhibited d-spacing at similar 2 θ values, but the relative intensity of these peaks was higher in the polymeric composites. FTIR analysis indicates that there were intermolecular hydrogen bonding and esterification between the drug and polymer in the polymeric composites. Conclusion: The changes that occurred in ciprofloxacin indicate increase in stability, decrease in solubility and delayed release of the drug from polymeric composites which could facilitate the formulation of a sustained release form of the drug. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.


PubMed | Kanak Manjari Institute of Pharmaceutical science
Type: Journal Article | Journal: World journal of diabetes | Year: 2015

Because of the intimate association of obesity with type 2 diabetes mellitus (T2DM), during the last two decades, extensive research work is being conducted to find out whether the coexistence of the two is a simple association or there is a positive correlating link between the two. In this article, an attempt has been made to collect and analyse the recent developments in this field and to arrive at a conclusion on the subject. The possible role of several important factors (obtained from adipocytes/not of adipocyte origin) in linking the two has been discussed in detail. Some of the agents, specifically adiponectin, are beneficial (i.e., reduce the incidence of both), while others are harmful (i.e., increase their incidence). From the analysis, it appears that obesity and T2DM are intimately linked.


PubMed | Kanak Manjari Institute of Pharmaceutical science
Type: Journal Article | Journal: World journal of gastrointestinal pathophysiology | Year: 2015

Due to the grave pathological role of obesity, worldwide research is being continued to find out the causative factors involved in it. Recent advances in this field reveal a possible relationship between the compositional pattern of gut microbiota and genesis of obesity. Several study results have shown that short-chain fatty acids (SCFAs, microbiota-induced fermentation products) and lipopolysaccharides (LPS, an integral component of Gram negative microorganisms) play the key role in linking the two. Though several SCFAs are produced as microbiota-fermentation products, three of them, i.e., butyrate, propionate and acetate have been found to be definitely involved in obesity; though individually they are neither purely obesogenic nor antiobesogenic. Out of these, butyrate and propionate are predominantly antiobesogenic. Butyrate, though a major energy source for colonocytes, has been found to increase mitochondrial activity, prevent metabolic endotoxemia, improve insulin sensitivity, possess anti-inflammatory potential, increase intestinal barrier function and protect against diet-induced obesity without causing hypophagia. Propionate has been found to inhibit cholesterol synthesis, thereby antagonizing the cholesterol increasing action of acetate, and to inhibit the expression of resistin in adipocytes. Moreover, both these SCFAs have been found to cause weight regulation through their stimulatory effect on anorexigenic gut hormones and to increase the synthesis of leptin. Unlike butyrate and propionate, acetate, which is substantially absorbed, shows more obesogenic potential, as it acts as a substrate for hepatic and adipocyte lipogenesis. High fat diet increases the absorption of LPS, which, in turn, has been found to be associated with metabolic endotoxemia and to induce inflammation resulting in obesity. Multiple independent and interrelated mechanisms have been found to be involved in such linking processes which are discussed in this review work along with some possible remedial measures for prevention of weight gain and obesity.

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