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Yang J.,Xinxiang Medical University | Yang J.,Kamp Institute for Medical Research | Yang J.,Yangzhou University | Li P.,Xinxiang Medical University | And 9 more authors.
Peptides | Year: 2011

Arginine vasopressin (AVP), which is synthesized and secreted in the hypothalamic paraventricular nucleus (PVN), is the most important bioactive substance in the pain modulation. Our pervious study had shown that AVP plays an important role in pain modulation in caudate nucleus (CdN). The experiment was designed to investigate the source of AVP in CdN by the nucleus push-pull perfusion and radioimmunoassay. The results showed that: (1) pain stimulation increased the AVP concentration in the CdN perfusion liquid, (2) PVN decreased the effect of pain stimulation which was stronger in both sides than in one side of PVN cauterization; and (3) L-glutamate sodium would excited the PVN neurons by the PVN microinjection that could increase the AVP concentration in the CdN perfusion liquid. The data suggested that AVP in the CdN might come from the PVN in the pain process, i.e., AVP in the PVN might be transferred to the CdN to participate in the pain modulation. © 2010 Elsevier Inc. All rights reserved.


Zhou L.-Y.,Xinxiang Medical University | Zhou L.-Y.,KAMP Institute for Medical Research | Yang J.,Xinxiang Medical University | Yang J.,KAMP Institute for Medical Research | And 6 more authors.
Asian Journal of Chemistry | Year: 2012

The specific synthetic route to synthesize the actarit (4-acetyl-amino phenylacetic acid) by acylation was designed in which p-amino benzaldehyde (a) and nitromethane (b) was used as the raw materials, through Knoevenagel reaction. The selective reduction of potassium borohydride and p-amino phenylacetic acid to be oxidated under the acidic conditions. After optimization of the synthetic conditions, the yield of each step was more than 85 %. The synthesized compound was confirmed by the elemental analysis and nuclear magnetic resonance. Because of the readily available raw materials, simple operations, high yield and avoiding highly toxic reagents in the synthesis of actarit (4-acetyl-amino phenylacetic acid), the synthetic route is suitable for the industrial production.


Yang J.,Xinxiang Medical University | Yang J.,Kamp Institute for Medical Research | Yang J.,Yangzhou University | Pan Y.-J.,Xinxiang Medical University | And 7 more authors.
Peptides | Year: 2011

Our previous studies have demonstrated that oxytocin (OXT) in the central nervous system plays a role in pain modulation. Many studies have found that caudate nucleus (CdN) enriches OXT and OXT receptors by the methods of historadioautograph and gene expression. The communication was designed to investigate OXT effect in the rat CdN on pain modulation. The results showed that (1) intra-CdN microinjection of OXT receptor antagonist, desGly-NH 2, d(CH 2) 5[d-Tyr 2, Thr-sup-4]OVT decreased the pain threshold, whereas the local administration of OXT increased the pain threshold in a dose-dependent manner; (2) OXT receptor antagonist can attenuate the analgesic role induced intra-CdN administration of OXT; and (3) pain stimulation could increase OXT concentration in the CdN perfusion liquid. The data suggested that OXT in the CdN was involved in this pain process via OXT receptors. © 2011 Elsevier Inc. All rights reserved.


Yang J.,Xinxiang Medical University | Yang J.,Kamp Institute for Medical Research | Yang J.,Yangzhou University | Liang J.-Y.,Xinxiang Medical University | And 8 more authors.
Peptides | Year: 2011

Our pervious study has demonstrated that the hypothalamic supraoptic nucleus (SON) plays a role in pain modulation. Oxytocin (OXT) and arginine vasopressin (AVP) are the important hormones synthesized and secreted by the SON. The experiment was designed to investigate which hormone was relating with the antinociceptive role of the SON in the rat. The results showed that (1) microinjection of l-glutamate sodium into the SON increased OXT and AVP concentrations in the SON perfusion liquid, (2) pain stimulation induces OXT, but not AVP release in the SON, and (3) intraventricular injection (pre-treatment) with OXT antiserum could inhibit the pain threshold increase induced by SON injection of l-glutamate sodium, but administration of AVP antiserum did not influence the antinociceptive role of SON stimulation. The data suggested that the antinociceptive role of the SON relates to OXT rather than AVP. © 2011 Elsevier Inc. All rights reserved.


Yang J.,Xinxiang Medical University | Yang J.,Kamp Institute for Medical Research | Yang J.,Yangzhou University | Liang J.-Y.,Xinxiang Medical University | And 6 more authors.
Peptides | Year: 2011

Periaqueductal gray (PAG) plays a very important role in pain modulation through endogenous opiate peptides including leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep) and dynorphin A 1-13 (DynA1-13). Our pervious study has demonstrated that intra-PAG injection of oxytocin (OXT) increases the pain threshold, and local administration of OXT receptor antagonist decreases the pain threshold, in which the antinociceptive role of OXT can be reversed by pre-PAG administration of OXT receptor antagonist. The experiment was designed to investigate the effect of OXT on endogenous opiate peptides in the rat PAG during the pain process. The results showed that (1) the concentrations of OXT, L-Ek, M-Ek and β-Ep, not DynA1-13 in the PAG perfusion liquid were increased after the pain stimulation; (2) the concentrations of L-Ek, M-Ek and β-Ep, not DynA1-13 in the PAG perfusion liquid were decreased by the OXT receptor antagonist; (3) the increased pain threshold induced by the OXT was attenuated by naloxone, an opiate receptor antagonist; and (4) the concentrations of L-Ek, M-Ek and β-Ep, not DynA1-13 in the PAG perfusion liquid were increased by exogenous OXT administration. The data suggested that OXT in the PAG could influence the L-Ek, M-Ek and β-Ep rather than DynA1-13 to participate in pain modulation, i.e. OXT in the PAG participate in pain modulation by influencing the L-Ek, M-Ek and β-Ep rather than DynA1-13. © 2011 Elsevier Inc. All rights reserved.

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