Petropavlovsk-Kamchatskiy, Russia

Kamchatka State Technical University
Petropavlovsk-Kamchatskiy, Russia
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Klochkova T.A.,Kongju National University | Klochkova T.A.,Kamchatka State Technical University | Klochkova N.G.,Kamchatka State Technical University | Yotsukura N.,Hokkaido University | Kim G.H.,Kongju National University
Algae | Year: 2017

Morphological, molecular and chromosomal studies were carried out on Tauya basicrassa, an endemic kelp species distributed on the northern continental coast of the Sea of Okhotsk in Russia. The sporophytes of T. basicrassa grow up to 3-6 m long, 1.8-2.2 m wide, and 6.5-7 kg wet weight. The thallus has a blade with very thick narrow basal portion and thinner and much broader upper portion, which usually splits into 3 bullated lobes. A dwarf laminariacean alga, which did not show any morphological similarity to the other species of the order Laminariales, was found from the same locality. The blade of this alga is thin and soft, reached 26-34 cm long and 6-6.5 cm wide and had 4 longitudinal rows of bullations that covered the entire blade. Molecular analysis showed that the dwarf alga has 100% sequence identity in plastid-encoded RuBisCo spacer, mitochondrial cytochrome c oxidase subunit 1 and nuclear-encoded rDNA genes with normal sporophytes of T. basicrassa, indicating that they are different life forms of the same species. Fluorescent DAPI staining showed that the nucleus in the normal sporophyte was 50-65% larger than those of the dwarf ones. Chromosome count using acetocarmine staining showed n = ca. 20 for the normal sporophytes of T. basicrassa and n = ca. 10 for the dwarf one. These results suggest that the dwarf thallus is a haploid parthenosporophyte of T. basicrassa, which developed in nature. This is the first evidence of parthenosporophytes of the laminariacean algae occurring naturally in the field. © 2017 The Korean Society of Phycology.

Mandrikova O.,RAS Institute of Radio Engineering and Electronics | Zhizhikina E.,Kamchatka State Technical University
E3S Web of Conferences | Year: 2016

The paper presents our software system for estimation the degree of disturbance of the geomagnetic field. The system automatically classifies registered geomagnetic data and determines the state of the geomagnetic field for the current day. The results of approbation of the system showed the prospect of its application in problems of estimation and prediction of space weather. The system allows us to allocate weak disturbances of the geomagnetic field, which may occur before strong magnetic storms. © The Authors, published by EDP Sciences, 2016.

Description of larvae of three species: Pandalus eous, P. goniurus and P. tridens (family Pandalidae) from planktonic samples taken in the Okhotsk Sea, Avacha Gulf, and in the Bering Sea is given. Morphological features of larvae for the purpose of their identification are compared. Features which are not subject to significant intraspecific variation, and which is useful for the separation of these species in larval period are discussed. The most important features for identification of larvae of these species may be the structure of the rostrum, the presence or absence of denticles on the carapace and abdomen, the number of setae on different legs. The most reliable feature for the separation of larvae of these species into stages of zoea is the structure of the maxilla. A key to identify of species in larval stages and drawings of separate stages are given. © 2017 Magnolia Press.

Klimova A.V.,Kamchatka State Technical University | Klochkova T.A.,Kongju National University
Russian Journal of Marine Biology | Year: 2017

This paper describes the formation of gametophytes and the early stages of the development of sporophytes in the kelp seaweed Alaria angusta from Kamchatka. To establish laboratory cultures we used zoospores obtained from A. angusta sporophytes collected on October 29, 2014. The gametophytes were grown under different conditions: at 6–7°С and natural light and at 10°С and illumination with cool white fluorescent bulbs, 30 μmol photon m–2 s–1, 12: 12 h L: D cycle. In the first case (natural light, 6–7°С), the vegetative growth of male and female gametophytes lasted for more than 4 months; maturation of sexual products occurred 144 days after germination of the embryospores. In the second case (artificial light, 10°С), rapid development of the gametophytes occurred; the first juvenile sporophytes appeared on the 10th day after the zoospores settled onto the substrate. Our data contribute to understanding of the regulatory effect of temperature and light on the peculiarities of species vegetation in the natural environment and formation of the age structure of the species population, one particular feature of this process is the continuous appearance of juvenile sporophytes in the warm period of the year. © 2017, Pleiades Publishing, Ltd.

Klochkova T.A.,Kongju National University | Klochkova N.G.,Kamchatka State Technical University | Kim G.H.,Kongju National University
Russian Journal of Marine Biology | Year: 2017

Molecular phylogenetic tools are often useful in distinguishing cryptic species with similar morphologies, but they can also be helpful in identifying morphotypes of a species, which displays completely different shapes. We performed molecular-phylogenetic analysis of supra-tidal green algae in the Kamchatka peninsula that belong to the order Prasiolales (Trebouxiophyceae, Chlorophyta). Based on rbcL sequences results, two new species were recorded for the first time in Kamchatka. Approximately 1.4% of the field-collected Rosenvingiella constricta had a unique uniseriate hood-like blade shape, although their rbcL sequences were 100% identical with typical multiseriate filamentous plants. Kamchatka’s population of R. constricta showed 99.4–99.6% identity in rbcL sequences with the populations from Canada and New Zealand. Another similar-looking Rosenvingiella species collected from the same locality had 93.5% identity of the rbcL gene sequence with R. constricta. Morphological and geographical analyses also suggested that this species might be a new species of the genus Rosenvingiella. Prasiola delicata was recorded for the first time in Kamchatka. The Kamchatka population of P. delicata showed 100% identity in rbcL gene sequence with the population from Vancouver, but differed from the Canadian population morphologically. © 2017, Pleiades Publishing, Ltd.

Shulyupin A.N.,Russian Academy of Sciences | Chermoshentseva A.A.,Kamchatka State Technical University
Thermophysics and Aeromechanics | Year: 2015

A mathematical model was developed for calculation of steam-water flow in a geothermal well for the feeding interval. The model assumes a variable mass flow rate over the channel length. The basis for this model are the flow continuity equation, momentum and energy conservation equations, taken with account for variable mass flow. The model was implemented as a computer code suitable for calculation of flow parameters upstream (downward the top level of the feeding zone). Then this model was applied for wells in the Mutnovskii geothermal field, this revealed a geyser-type mechanism of flow instabilities with the pressure oscillation period about ten minutes. The remedy for these oscillations was offered. © 2015, Pleiades Publishing, Ltd.

Mandrikova O.V.,Kamchatka State Technical University | Zhizhikina E.A.,Kamchatka State Technical University
Computer Optics | Year: 2015

We introduce a new method for estimating the geomagnetic field. The method is based on a combination of a wavelet transform with radial basis neural networks. In the method, the recorded geomagnetic field variations are decomposed into different-scale components and the degree of disturbance of each component is estimated, enabling the conclusion about the field state. For the verification of the method, we used geomagnetic data from the "Paratunka" station (Paratunka, Kamchatka region, data registration is carried out by IKIR FEB RAS). Analysis of the spectral-temporal characteristics of geomagnetic field variations during periods of moderate and strong magnetic storms was performed. Weak perturbations were detected in the geomagnetic field before the storms. The obtained results have confirmed the effectiveness of the proposed method. © 2015, Institution of Russian Academy of Sciences. All rights reserved.

Pisareva N.A.,Russian Academy of Sciences | Klochkova N.G.,Kamchatka State Technical University
Russian Journal of Marine Biology | Year: 2013

The results of taxonomic revision of the red algal genus Neoabbottiella from the Russian Far Eastern seas are presented and the differences of Neoabbottiella from the other blade-like red algae are discussed. Descriptions of three species are given, including N. araneosa (type species) and two newly described species, N. valentinae sp. nov. and N. decipiens sp. nov. The newly described species differ from N. araneosa in morphogenesis, structure, and localization of gonimoblasts on the blade, as well as some features of vegetative anatomy. A key to the species of Neoabbottiella, illustrations of their external and internal structures and some data on their distribution and ecology are given. © 2013 Pleiades Publishing, Ltd.

Mandrikova O.V.,Kamchatka State Technical University | Bogdanov V.V.,Russian Academy of Sciences | Solov'ev I.S.,Kamchatka State Technical University
Geomagnetism and Aeronomy | Year: 2013

Variations in geomagnetic field data at different spectral frequencies and with different periods are observed during increased geomagnetic activity. The formed local structures depend on the field disturbance and contain information on the magnetic storm intensity and character of development. Numerical solutions and algorithms based on wavelet transforms, which make it possible to "automatically" detect periods of increased geomagnetic activity and identify and analyze the structures forming this process, have been proposed in order to study the time characteristics of geomagnetic field variations, using the H component as an example. The separated components, characterizing disturbances, make it possible to estimate variations in the field energy characteristics. An analysis of the constructed wavelet images makes it possible to trace the dynamics of variations in the H component the day before and during a magnetic storm. © 2013 Pleiades Publishing, Ltd.

Klochkova T.A.,Kamchatka State Technical University | Kwak M.S.,Kongju National University | Han J.W.,Kongju National University | Motomura T.,Hokkaido University | And 2 more authors.
Algae | Year: 2013

A new cold-adapted Arctic strain of Haematococcus pluvialis from Blomstrandhalvøya Island (Svalbard) is described. This strain is predominantly always in non-motile palmelloid stage. Transmission electron microscopy showed the presence of very thick cell wall and abundant lipid vesicles in the palmelloids, including red and green cells. The external morphology of the non-motile palmelloid and motile bi-flagellated cells of our strain is similar to H. pluvialis; however it differs from H. pluvialis in physiology. Our strain is adapted to live and produce astaxanthin in the low temperature (4- 10°C), whilst the usual growth temperature for H. pluvialis is between 20-27°C. Phylogenetic analysis based on 18S rRNA gene data showed that our strain nested within the Haematococcus group, forming a sister relationship to H. lacustris and H. pluvialis, which are considered synonymous. Therefore, we identified our Arctic strain as H. pluvialis. © The Korean Society of Phycology.

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