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Beta M.,Kamalnayan Bajaj Institute for Research in Vision and Opthalmology | Beta M.,SASTRA University | Krishnakumar S.,Kamalnayan Bajaj Institute for Research in Vision and Opthalmology | Elchuri S.V.,Kamalnayan Bajaj Institute for Research in Vision and Opthalmology | And 2 more authors.
Analytical Sciences | Year: 2015

Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 μL healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number. © The Japan Society for Analytical Chemistry.

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