Warangal, India
Warangal, India

Kakatiya University is a public university located in Warangal in the Indian state of Telangana.The university offers about 120 programs at undergraduate and postgraduate levels in the faculties of arts, science, commerce and business management, social science, education, engineering and pharmaceutical science with constituent and affiliated colleges spread over four districts of Telangana. The faculties include 248 teaching staff and 622 non-teaching staff. Wikipedia.

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Samala S.,Kakatiya University | Veeresham C.,Kakatiya University
Phytotherapy Research | Year: 2016

The effect of boswellic acids (BA) and andrographolide (AD) on the pharmacokinetics and pharmacodynamics of glyburide in normal as well as in streptozotocin-induced diabetic rats was studied. In normal and diabetic rats, the combination of glyburide with BA or AD increased significantly (p < 0.01) all the pharmacokinetic parameters, such as Cmax, AUC0-n, AUCtotal, t1/2, and mean residence time, and decreased the clearance, Vd, markedly as compared with the control group. In rat liver, microsomes BA and AD have shown CYP3A4 inhibitory activity significantly (p < 0.01), compared with the vehicle group. The increase in hypoglycemic action by concomitant administration of glyburide with BA or AD was more in diabetic rats than when the drugs were used singly and with the control group, which suggests the enhancement of glucose reduction capacity of glyburide in diabetic rats along with BA or AD. In PK/PD modeling of BA and AD with glyburide, the predicted PK and PD parameters are in line with the observed PK and PD parameters. The results revealed that BA and AD led to the PK/PD changes because of glyburide-increased bioavailability and because of the inhibition of CYP3A4 enzyme. In conclusion, add-on preparations containing BA or AD may increase the bioavailability of glyburide, and hence the dose should be monitored. Copyright © 2016 John Wiley & Sons, Ltd.

Dudhipala N.,Kakatiya University | Veerabrahma K.,Kakatiya University
Drug Delivery | Year: 2016

Candesartan cilexetil (CC) is used in the treatment of hypertension and heart failure. It has poor aqueous solubility and low oral bioavailability. In this work, CC loaded solid lipid nanoparticles (CC-SLNs) were developed to improve the oral bioavailability. Components of the SLNs include either of trimyristin/tripalmitin/tristearin, and surfactants (Poloxamer 188 and egg lecithin E80). The CC loaded nanoparticles were prepared by hot homogenization followed by ultrasonication method. The physicochemical properties, morphology of CC-SLNs were characterized, the pharmacokinetic and pharmacodynamic behaviour of CC-SLNs were evaluated in rats. Stable CC-SLNs having a mean particle size of 180-220 nm with entrapment efficiency varying in between 91-96% were developed. The physical stability of optimized formulation was studied at refrigerated and room temperature for 3 months. Further, freeze drying was tried for improving the physical stability. DSC and XRD analyses indicated that the drug incorporated into SLN was in amorphous form but not in crystalline state. The SLN-morphology was found to be nearly spherical by electron microscopic studies. Pharmacokinetic results indicated that the oral bioavailability of CC was improved over 2.75-fold after incorporation into SLNs. Pharmacodynamic study of SLNs in hypertensive rats showed a decrease in systolic blood pressure for 48 h, while suspension showed a decrease in systolic blood pressure for only 2 h. Taken together, these effects are due to enhanced bioavailability coupled with sustained action of CC in SLN formulation. Thus, the results conclusively demonstrated the role of CC-SLNs for a significant enhancement in oral bioavailability along with improved pharmacodynamic effect. © 2014 Informa Healthcare USA, Inc.

Bedada S.K.,Kakatiya University | Neerati P.,Kakatiya University
Phytotherapy Research | Year: 2016

The purpose of the present study was to investigate the effect of resveratrol (RSV) pretreatment on CYP2E1 enzyme activity and pharmacokinetics of chlorzoxazone (CHZ) in healthy human volunteers. The open-label, two period, sequential study was conducted in 12 healthy human volunteers. A single dose of RSV 500 mg was administered once daily for 10 days during treatment phase. A single dose of CHZ 250 mg was administered during control and after treatment phases under fasting conditions. The blood samples were collected after CHZ dosing at predetermined time intervals and analyzed by HPLC. RSV pretreatment significantly enhanced the maximum plasma concentration (Cmax), area under the curve (AUC) and half life (T1/2) and significantly decreased elimination rate constant (Kel), apparent oral clearance (CL/F) and apparent volume of distribution (Vd/F) of CHZ as compared to that of control. In addition, RSV pretreatment significantly decreased the metabolite to parent (6-OHCHZ/CHZ) ratios of Cmax, AUC and T1/2 and significantly increased the Kel ratio of 6-OHCHZ/CHZ, which indicated the reduced formation of CHZ to 6-OHCHZ. The results suggest that the altered CYP2E1 enzyme activity and pharmacokinetics of CHZ might be attributed to RSV mediated inhibition of CYP2E1 enzyme. Thus, there is a potential pharmacokinetic interaction between RSV and CHZ. The inhibition of CYP2E1 by RSV may provide a novel approach for minimizing the hepatotoxicity of ethanol. Copyright © 2015 John Wiley & Sons, Ltd.

Rajendra Prasad A.,Kakatiya University | Nageswara Rao A.S.,Kakatiya University
Materials Today: Proceedings | Year: 2016

Man has been using several plants and herbs even from pre-historic times to cure various ailments. In the modern world, popularity of various medicinal plants and plant therapies in treating various diseases is increasing. In the present study trace elemental analysis employing Particle Induced X-ray Emission (PIXE) technique is carried out in two selected medicinal plants used in the preparation of anti-diabetic drugs. The elements K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Br, Rb, Sr and Pb are established by using GUPIX software and these elemental concentrations are quantified in (μg/g) ppm level. Comparisons of previous measurements for similar materials found in the literature were presented. The present measurements of elemental concentrations in these medicinal plants will be useful to set new standards for prescribing dosage and duration of administration of these herbal drugs to diabetic patients. © 2016 Elsevier Ltd. All rights reserved.

Rani Roy B.,Kakatiya University | Nageswara Rao A.S.,Kakatiya University
Materials Today: Proceedings | Year: 2016

The work presents the X-ray absorption fine structure (XAFS) technique for measuring the X-ray absorption coefficient of NiCO3 in the X-ray energy range of 8271.2 to 8849.4 eV using scanning EXAFS beam line (BL-09) at Indus-2 synchrotron radiation source facility, Raja Ramanna Centre for Advanced Technology (RRCAT) at Indore, India. The result represents the absorption coefficient data for NiCO3 in the XAFS region of K-edge of Ni. However, the results are compared to theoretical predictions obtained from X-COM and FFAST methods. There is a maximum deviation which is found exactly near the K-edge jump and decreases as we move away from the absorption edge. © 2016 Elsevier Ltd. All rights reserved.

Narsimhulu M.,Kakatiya University | Hussain K.A.,Kakatiya University
Materials Today: Proceedings | Year: 2016

The thermal behavior and Raman spectroscopic properties of potassium diaqua bis(oxalato)nickelate(II) tetrahydrate {K2[Ni(C2O4)2(H2O)2]·4H2O} crystals have been investigated. This compound crystallizes in the monoclinic system with P21/c space group and shows one-dimensional (1D) chain structure. The microstructural features on the surface of the single crystals were determined using a Field Emission Scanning Electron Microscope (FE-SEM). Raman spectroscopy was used to study the vibrational modes of the compound. Thermal studies indicate that the compound is stable up to 280°C. © 2016 Elsevier Ltd.

Jadhav R.,Kakatiya University | Puchchakayala G.,Kakatiya University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

The aim of the current study was to investigate the oral antidiabetic activity and antihyperlipidemic activity of four flavonoids: Boswellic acid (1), Ellagic acid (2), Quercetin (3), Rutin (4). Normoglycemic and STZ-nicotinamide induced diabetic rats were treated with these flavonoids with 50 mg/kg, 100mg/kg and the hypoglycemic and antidiabetic effects in acute experiments were determined. After 14 days of administration to STZnicotinamide induced diabetic rats, flavonoids induced a significantly diminishing of total cholesterol, Triglyceride compared with the control group (p < 0.001). To find out the probable mechanism of action of selected flavonoids as a antidiabetic agents i) glucose transport inhibition activity, and ii) glucose uptake by isolated rat hemi-diaphragm were estimated. Selected flavonoids increased the uptake of glucose by rat hemi-diaphragm significantly (P<0.001), There was a significant decrease in glucose transport activity (P<0.05. Compounds 4, 3, and 2 were found most active in both experiments in comparison with control group (p < 0.001).

Umate P.,Kakatiya University
Plant Signaling and Behavior | Year: 2011

The enzymes called lipoxygenases (LoXs) can dioxygenate unsaturated fatty acids, which leads to lipoperoxidation of biological membranes. This process causes synthesis of signaling molecules and also leads to changes in cellular metabolism. LoXs are known to be involved in apoptotic (programmed cell death) pathway, and biotic and abiotic stress responses in plants. Here, the members of LoX gene family in arabidopsis and rice are identified. The arabidopsis and rice genomes encode 6 and 14 LoX proteins, respectively, and interestingly, with more LoX genes in rice. The rice LoXs are validated based on protein alignment studies. This is the first report wherein LoXs are identified in rice which may allow better understanding the initiation, progression and effects of apoptosis, and responses to bitoic and abiotic stresses and signaling cascades in plants. © 2011 Landes Bioscience.

Lipid bodies store oils in the form of triacylglycerols. Oleosin, caleosin and steroleosin are unique proteins localized on the surface of lipid bodies in seed plants. This study has identified genes encoding lipid body proteins oleosin, caleosin and steroleosin in the genomes of five plants: Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, Selaginella moellendorffii and Physcomitrella patens. The protein sequence alignment indicated that each oleosin protein contains a highly-conserved proline knot motif, and proline knob motif is well conserved in steroleosin proteins, while caleosin proteins possess the Dx[D/N]xDG-containing calcium-binding motifs. The identification of motifs (proline knot and knob) and conserved amino acids at active site was further supported by the sequence logos. The phylogenetic analysis revealed the presence of magnoliophyte- and bryophyte-specific subgroups. We analyzed the public microarray data for expression of oleosin, caleosin and steroleosin in Arabidopsis and rice during the vegetative and reproductive stages, or under abiotic stresses. Our results indicated that genes encoding oleosin, caleosin and steroleosin proteins were expressed predominantly in plant seeds. This work may facilitate better understanding of the members of lipid-body-membrane proteins in diverse organisms and their gene expression in model plants Arabidopsis and rice. © 2012 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China.

Light-harvesting antenna system possesses an inherent property of photoprotection. The single-helix proteins found in cyanobacteria play role in photoprotection and/or pigment metabolism. The photoprotective functions are also manifested by the two- and four-helix proteins. The photoprotection mechanism evolved earlier to the mechanism of light-harvesting of the antenna complex. Here, the light-harvesting complex genes of photosystems I and II from Arabidopsis are enlisted, and almost similar set of genes are identified in rice. Also, the three-helix early light-inducible proteins (ELIPs), two-helix stress-enhanced proteins (SEPs), and one-helix high light-inducible proteins [one-helix proteins (OHPs)] are identified in rice. Interestingly, two independent genomic loci encoding PsbS protein are also identified with implications on additional mode of non-photochemical quenching (NPQ) mechanism in rice. A few additional LHC-related genes are also identified in rice (LOC_Os09g12540, LOC_Os02g03330). This is the first report of identification of light-harvesting complex genes and light-inducible genes in rice.

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