Dhanapal R.,Kakatiya Institute of Pharmaceutical science |
Kumar A.S.,SVCP Sri Sainathnagar |
Ratna J.V.,Andhra University
Asian Pacific Journal of Tropical Biomedicine | Year: 2012
Objective: To study the effect of antioxidant activity of the ethanol extract of whole plant of Ammania baccifera, Linn (EEAB). Methods: To investigate in rats against carbon tetrachloride (CCl4) induced erythrocyte damage. Simultaneous intraperitoneal administration of the crude extracts (600 or 800 mg/kg body weight/day) with carbon tetrachloride (1ml/kg of body weight) to rats for alternate days of two weeks protected the loss of functional integrity and membrane lipid alteration in red blood cells induced by oxidative stress. Results: EEAB inhibited the accumulation of lipid peroxidation products in the plasma as well as maintained the activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalase. The extracts further had the ability to decrease the membrane fluidity induced by carbon tetrachloride. Conclusion: It can therefore be suggested that the whole plant of Ammania baccifera, Linn possess an erythrocyte protective activity against drug induced oxidative stress. These findings also provide a rationale for further studies on isolation of active principles and its pharmacological evaluation. © 2012 Asian Pacific Tropical Biomedical Magazine.
Kiran G.,Kakatiya Institute of Pharmaceutical science |
Rajyalakshmi G.,Kakatiya University
Toxicological and Environmental Chemistry | Year: 2013
The aim of the present study was to evaluate the hepatoprotective activity of 1-(4-(dimethylamino)benzylidene)-5-(2-oxoindolin-3-ylidene) thiocarbohydrazone, a novel isatin derivative against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Hepatic damage was induced by administration of CCl4 (1 ml/kg, b.w., p.o.) in combination with liquid paraffin (1:1) as a single dose. The hepatotoxic rats were treated with test compound at doses of 50 or 100 mg/kg for three days and liver damage biomarkers, including activities of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), serum alkaline phosphatase (ALP), and levels of total serum bilirubin (TB) measured in blood samples. Results demonstrated that treatment with test compound at doses of 50 or 100 mg/kg to hepatotoxic rats produced a significant dose-dependent reduction of elevated SGOT, SGPT, ALP activities and TB levels indicating a hepatoprotective effect that was confirmed by histopathological examination of liver tissues. The study results confirmed the hepatoprotective activity of 1-(4-(dimethylamino)benzylidene)-5-(2-oxoindolin-3-ylidene) thiocarbohydrazone in rats. © 2014 Taylor & Francis.
Gangarapu K.,Kakatiya Institute of Pharmaceutical science |
Gangarapu K.,Jawaharlal Nehru Technological University |
Thumma G.,SVS Group of Institutions |
Manda S.,Kakatiya University
Journal of Chemistry | Year: 2014
The present study was aimed at evaluating the cardioprotective effect of novel synthetic N'', N''' -bis[5-methyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene] carbonohydrazide derivative, by estimating the various biomarkers like creatine kinase-myoglobin (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and triglycerides (TG) in plasma and antioxidants like catalase, superoxide dismutase in heart tissue homogenate, and histopathological examination of heart tissues. The results showed the significant (P < 0.05) dose dependent decrease in elevated cardiotoxic biomarkers CK-MB, LDH, AST, and TG levels. The histopathological studies of heart tissues showed mild degeneration of muscle bundles and less interstitial edematous changes. The results showed the significant (P < 0.05) dose dependent increase in antioxidant enzymes catalase and superoxide dismutase in heart tissue homogenates. These observations enable us to conclude that N'', N''' -bis[5-methyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene]carbonohydrazide has cardioprotective activity against doxorubicin induced cardiotoxicity. © 2014 Salma Tabassum et al.
Burra M.,Kakatiya Institute of Pharmaceutical science |
Jukanti R.,St Peters Institute Of Pharmaceutical Science |
Janga K.Y.,St Peters Institute Of Pharmaceutical Science |
Sunkavalli S.,St Peters Institute Of Pharmaceutical Science |
And 3 more authors.
Advanced Powder Technology | Year: 2013
The current oral therapy with raloxifene hydrochloride (RXH) is less effective due to its poor bioavailability (only 2%). Henceforth, an attempt was made to investigate the utility of triglyceride (trimyristin, tripalmitin and tristearin) based solid lipid nanoparticles (SLNs) for improved oral delivery of RXH. The SLN formulations prepared were evaluated for particle size, zeta potential and % entrapment and the optimized formulation was lyophilized. Solid state characterization studies unravel the transformation of RXH to amorphous or molecular state from the native crystalline form. Further the in situ perfusion studies carried out in rat intestine reveal the potential of SLN for enhanced permeation of raloxifene HCl across gastrointestinal barrier. To derive the conclusions, in vivo pharmacokinetic study was conducted in rats to assess the bioavailability of RXH from SLN formulation compared to drug suspension. Overall a twofold increase in bioavailability with SLN formulations confer their potential for improved oral delivery of RXH. © 2012 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder.
Cherala S.,Kakatiya University |
Lingabathula H.,Kakatiya Institute of Pharmaceutical science |
Ganta R.,Kakatiya Institute of Pharmaceutical science |
Ampati S.,Kakatiya Institute of Pharmaceutical science |
Manda S.,Kakatiya University
E-Journal of Chemistry | Year: 2012
In the present investigation we have synthesized a series of new 1-[3-(4-substitutedphenyl)-5-phenyl-4H-1,2,4-triazol-4-yl]urea and 1-[3-(4-substitutedphenyl)-5-phenyl-4H-1,2,4-triazol-4-yl]thiourea derivatives (4Ia - 4IId). The newly synthesised derivatives were characterized by using the data of IR, 1 H NMR and Mass Spectral analysis. Thus synthesised and characterized targetted compounds were further screened for their anti-inflammatory activity by using Carrageenan - induced paw edema rat model. Among all the newly synthesized derivatives, Compounds 4Ia-4Ic and Compounds 4IIa-4IId were reduced the inflammation very significantly (p <0.0001), thus these compounds showed promising anti-inflammatory activity and only one compound (4Id) showed moderate anti-inflammatory activity (p <0.05). © 2012 Hindawi Publishing Corporation.