Okanoue T.,Saiseikai Suita Hospital |
Shima T.,Saiseikai Suita Hospital |
Hasebe C.,Red Cross |
Karino Y.,Sapporo Kohseiren Hospital |
And 18 more authors.
Hepatology Research | Year: 2016
Aim: We analyzed the 5-year post-treatment response to peginterferon α-2a (PEG IFN-α-2a) in hepatitis B e-antigen (HBeAg) positive and negative chronic hepatitis B patients. Methods: One hundred and thirty-seven chronic hepatitis B (CHB) patients receiving 90 μg or 180 μg of PEG IFN-α-2a for 24 or 48 weeks in phase II or III studies were enrolled in the study, including 100 HBeAg positive patients and 37 HBeAg negative patients; 121 patients (88.4%) had genotype C. Results: Of the 137 patients, 94 received additional antiviral therapy because of viral reactivation and 43 did not receive any additional antiviral treatment during follow up. Five years upon PEG IFN-α-2a treatment, 32 patients (23.4%) who did not receive any additional antiviral agent after PEG IFN-α-2a therapy achieved a good response (normal serum alanine aminotransferase, low-level hepatitis B virus [HBV] DNA, and HBeAg negativity). Female sex and low HBV DNA levels by the end of treatment were independently associated with favorable 5-year post-treatment responses. Forty-eight-week administration of PEG IFN-α-2a showed a better response (26.4%) than 24-week administration (18.0%). Six patients (4.3%), four males and two females, cleared hepatitis B surface antigen (HBsAg) during the 5-year follow-up period. Conclusion: The 48-week administration of PEG IFN-α-2a achieved better biochemical and virological responses than the 24-week administration, particularly in younger females. The 5-year post-treatment response rate was 23.4%; however, more than two-thirds of the patients received additional antiviral therapy because of viral reactivation after PEG IFN-α-2a treatment. HBsAg clearance was noted in six patients (4.3%). PEG IFN-α-2a is effective in young female patients. © 2016 The Japan Society of Hepatology
Shiozaki K.,Kagoshima University |
Shiozaki K.,Kagoshima UniversityKagoshima |
Harasaki Y.,Kagoshima University |
Fukuda M.,Kagoshima University |
And 7 more authors.
Biochimie | Year: 2016
Sialidase Neu3b is an unique enzyme conserved in medaka and tilapia, but not in mammals. Previous study revealed that medaka Neu3b is localized at cytosol and is a ganglioside-specific sialidase. Neu3b functions, however, have not been understood, while Neu3a sialidase, which is widely conserved from human to fish, is known as a regulator of neurite formation. Here, we investigated the biological function of Neu3b for C2C12 myoblast cell differentiation. Bioinformatics analysis using genome browser revealed the presence of neu3b gene in some orders of fish species such as Beloniformes, Perciformes and Cyprinodontiformes. With the treatment of 2% horse serum, Neu3b-overexpression accelerated myoblast cell differentiation to myotubes accompanied with up-regulation of myogenesis biomarkers mRNA, myod and myog. Neu3b altered ganglioside composition in C2C12 cells results showing a decrease in GM2, and the increase of Lac-Cer, while desialylation of glycoproteins were not detected. Contrary to cell differentiation, Neu3b cell proliferation was suppressed in normal growth medium. To understand the mechanism of the alteration of cell differentiation and proliferation, phosphorylation of signal molecules in EGFR/ERK pathway was investigated. Neu3b induced a decline in phosphorylation of ERK and EGFR. Surprisingly, immuno-blot and real-time PCR analysis revealed that down-regulation of egfr gene could be involved in the acceleration of cell differentiation by Neu3b. These results suggested that Neu3b sialidase is a positive regulator for myoblast differentiation, similar with mammalian cytosolic sialidase Neu2. © 2016 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.
Funahashi A.,Kagoshima University |
Komatsu M.,Kagoshima University |
Furukawa T.,Kagoshima UniversityKagoshima |
Furukawa T.,Cancer Therapy and Research Center |
And 9 more authors.
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology | Year: 2016
Green fluorescent protein (GFP) from eel (Anguilla japonica) muscle (eelGFP) is unique in the vertebrates and requires bilirubin as a ligand to emit fluorescence. This study was performed to clarify the physiological function of the unique GFP. Investigation of susceptibility to oxidative stress was carried out using three types of cell lines including jellyfish (Aequorea coerulescens) GFP (jfGFP)-, or eel GFP (eelGFP)-expressing HEK293 cells, and control vector-transfected HEK293 cells. Binding of eelGFP to bilirubin was confirmed by the observation of green fluorescence in HEK293-eelGFP cells. The growth rate was compared with the three types of cells in the presence or absence of phenol red which possessed antioxidant activity. The growth rates of HEK293-CV and HEK293-jfGFP under phenol red-free conditions were reduced to 52 and 31% of those under phenol red. Under the phenol red-free condition, HEK293-eelGFP had a growth rate of approximately 70% of the phenol red-containing condition. The eelGFP-expressing cells were approximately 2-fold resistant to oxidative stress such as H2O2 exposure. The fluorescence intensity partially decreased or disappeared after exposure to H2O2, and heterogeneous intensity of fluorescence was also observed in isolated eel skeletal muscle cells. These results suggested eelGFP, but not jfGFP, coupled with bilirubin provided the antioxidant activity to the cells as compared to non-bound free bilirubin. © 2016 Elsevier Inc. All rights reserved.
Xu Q.,Kyoto University |
Yokotani T.,Kyoto University |
Sato K.,Kagoshima UniversityKagoshima |
Hori F.,Osaka Prefecture University
Journal of Nuclear Materials | Year: 2016
Electrical resistivity measurement is a useful experimental method for investigating the recovery of defects that are induced by irradiation in metals and alloys. In this study, an Fe-0.6%Cu alloy, used to model steel from old commercial reactor pressure vessels, was irradiated by neutrons at a low temperature range of 14–19 K with a dose of about 1.3 × 1020 neutrons/m2 (E > 0.1 MeV) in the Kyoto University Reactor (KUR); electrical resistivity measurement was performed during irradiation and after annealing of the irradiated sample from 20 K to 300 K to investigate the migration of point defects in the Fe-0.6%Cu alloy. The electrical resistivity was measured at 14–19 K. With the increase in the irradiation dose, the electrical resistivity increased linearly. Four peaks appeared at 70 K, 100 K, 150 K, and 260 K, in the change of electrical resistivity during annealing of the irradiated sample up to 300 K. The former two peaks were caused by the recombination of interstitials and vacancies, and the latter two peaks were caused by the formation of interstitial clusters and the migration of vacancies. Compared with previous electron irradiation results, the former two peaks represent new data, as does the ratio of recombination caused by close-pair and correlation to that caused by migrations of mixed-interstitials Fe[sbnd]Cu and vacancies decreased in neutron irradiation. © 2016 Elsevier B.V.
Zhao X.,Kagoshima UniversityKagoshima |
Zheng S.-H.,Saga University |
Arima S.,Saga University
Plant Production Science | Year: 2014
Nitrogen assimilation during seed filling limits the seed yield in soybean. Seed nitrogen dependence on either redistributed nitrogen or absorbed nitrogen from soil during seed filling shows varietal differences. The objective of this study was to investigate the timing of nitrogen enrichment for effective nitrogen assimilation. Two soybean cultivars Sachiyutaka and Tamahomare were sown in the pots filled with well-washed fine sand. The plants were well watered with nutrient solution containing 100 ppm nitrogen and other nutrient elements before and after the treatment. The treatments were conducted from reproductive stage R1 to R5 or from R5 to R7 by applying the nutrient solution with different nitrogen concentrations. The high nitrogen concentration from R1 to R5 delayed the decline in SPAD value and leaf nitrogen concentration and improved the seed yield performance in Sachiyutaka, whereas stimulated the decline in SPAD value and leaf nitrogen concentration and had no effect on seed yield in Tamahomare. However, high nitrogen concenntration during R5 to R7 delayed the decline in SPAD value and leaf nitrogen concentration and improved the yield performance more significantly in Tamahomare than in Sachiyutaka. The large seed yield increase by nitrogen enrichment during R5 to R7 in Tamahomare could be caused by both the high photosynthetic rate and vigorous nitrogen uptake during seed filling. These results suggested that the most effective timing of nitrogen enrichment during the reproductive growth period to increase seed yield varies with the cultivar due to the difference in the pattern of nitrogen assimilation. © 2014 by The Crop Science Society of Japan.