Kagoshima Immaculate Heart University is a private women university in Satsumasendai, Kagoshima, Japan. The predecessor of the school was founded in 1933, and it was chartered as a junior university in 1961. In 1994, it became a four-year college and adopted the present name. Wikipedia.
Takeshita H.,Kagoshima University |
Takeshita H.,Kagoshima Immaculate Heart University |
Takeshita H.,University of Shizuoka |
Horiuchi M.,Kagoshima University |
And 6 more authors.
Environmental Health and Preventive Medicine
Objectives To determine the impact of long-term voluntary exercise, representing habitual exercise for the prevention of lifestyle-related diseases, on glucose, lipid, and amino acid metabolism in mice. Methods Twenty-four mice aged 6 weeks were divided into three groups. Two groups (16 mice) were housed individually in either cages equipped with a running wheel (8 mice, exercising, Ex-mice) or without (8 mice, sedentary, Se-mice) for 24 weeks. The remaining group (8 mice) was sacrificed at 6 weeks of age. Biomarkers related to glucose, lipid, and amino acid metabolism were examined. Results Ex-mice ran voluntarily, predominantly in the dark. The distance per day peaked at 4 weeks and then decreased until 12 weeks to around the level seen at the beginning of the experimental period, and was maintained at 4.9 ± 0.2 km/day from 12 to 24 weeks. Ex-mice showed a similar adrenal weight and vitamin C content to Se-mice but had a significantly lower body weight and higher food intake. Ex-mice also showed a higher skeletal muscle weight, a lower white adipose tissue and liver weight, associated with lower plasma leptin and insulin-like growth factor-1 levels, and a lower hepatic triglyceride content. Analysis of plasma amino acids showed that Ex-mice had significantly higher phenylalanine, tyrosine, and glutamine levels, resulting in a significantly lower Fischer's ratio. Conclusions We present an animal model of long-term voluntary exercise under low stress. Findings related to the effects of long-term voluntary exercise on lipid, and amino acid metabolism in our mouse model indicate that such an exercise regimen may affect pathophysiological states related to appetite and behavior. © The Japanese Society for Hygiene 2011. Source
Kuchiiwa S.,Kagoshima University |
Kuchiiwa T.,Kagoshima University |
Kuchiiwa T.,Kagoshima Immaculate Heart University
Journal of Neuroscience Methods
Background: Currently, behavioral research of aggressiveness is often conducted with intraspecific intermale aggression tests. Intraspecific aggression is not detectable in early stages of psychiatric disorders or in female animals, except during the nursing period. New method: We developed a semi-automated apparatus (ARM: Aggression Response Meter) for measurement of aggressive biting behavior (ABB) in mice. The apparatus is loaded with computer-controlled sticks that stimulate the mouse through touch, inducing irritation and anger. When the mouse bites the sticks in anger, a load sensor attached to the sticks detects ABB dynamically. Changes in ABB were assessed with isolation-reared/re-socialized mice using the ARM, and additional isolation-reared mice were tested using both the ARM and the resident-intruder test, and then buspirone, a serotonin 1A receptor agonist, was administered. Results: ABB significantly increased during isolation rearing, and then significantly decreased throughout the re-socialization period; both changes were time-dependent. The ARM also detected ABB of female mice after 3 weeks of isolation rearing. Buspirone significantly inhibited aggressive behavior in both tests in a similar manner. Comparison with existing method: The ARM detects aggressiveness in psychiatric disorders at an earlier stage and in both male and female mice. Conclusions: ABB toward inanimate objects is a reliable paradigm that makes it possible to detect aggressiveness in the early stage of psychiatric disorders. The ARM is useful for the quantification of aggressiveness using the same individual repeatedly, and for objective evaluation of the effects of drugs on aggressiveness. The ARM can be used with both male and female mice. © 2014 Elsevier B.V. Source
Yamada K.,Gifu Municipal Hospital |
Tsunoda K.,Kagoshima Immaculate Heart University |
Kawai K.,Gifu University |
Ikeda T.,Gifu Municipal Hospital |
And 4 more authors.
Aim: Fenofibrate (FF) is known to block ATP synthesis in mitochondria by inhibiting electron transport at NADH-ubiquinone (UQ) oxidoreductase (complex I) in the respiratory chain. Methods: Toxicity to mitochondria of bezafibrate (BF) was compared with that of FF, focusing exclusively on their effects on impairment of the electron-transport and energy-transfer systems in oxidative phosphorylation, by experiments with isolated rat liver mitochondria and submitochondrial particles (SMP). Results: FF and BF had an uncoupling effect, enhancing ATP hydrolytic activity (latent ATPase) in mitochondria. DNP-stimulated ATPase activity was not suppressed by either agent, indicating no interference with the energy-transfer system. Both reagents inhibited NAD-linked respiration but did not affect succinate-linked respiration, indicating specific inhibition of electron-transport activity in complex I. NADH oxidase in SMP was strongly inhibited by FF, and activity was recovered by treatment with tetramethyl-p-phenylenediamine, indicating that the site of inhibition is not located at the NADH dehydrogenase portion but at the UQ site in complex I. Uncoupling and electron-transport inhibition by BF were noticeably weaker than those by FF. Conclusions: From these results it was concluded that BF and FF were toxic to mitochondria through the dual actions of an uncoupling effect and electron-transport inhibition. The toxicity of BF to mitochondrial function was found to be noticeably weaker than that of FF, implying that BF might be much less toxic than FF for long term in-vivo use as an antihyperlipidemic agent. © 2013 The Japan Diabetes Society. Source
Kimura K.,Niigata University |
Nakano K.,Niigata University |
Ohashi S.,Niigata University |
Takizawa K.,Niigata University |
Nakano T.,Kagoshima Immaculate Heart University
In Japan, fertilised chicken eggs are used to produce influenza vaccines; however, some eggs die and then contaminate the vaccine stock solution, which causes large economic losses and raises health concerns. Therefore, a non-destructive test that allows distinction of normal and unsuitable chicken eggs was developed, first using visible and near-infrared (VNIR) spectroscopy and subsequently a light-emitting diode (LED) light source. Eggs were sampled from an egg farm, and each of the eggs categorised as either normal or unsuitable using candling. Linear discriminant analysis was applied using wavelength absorbance data to distinguish between normal and unsuitable eggs. All of the optical absorbance values of the normal eggs were found to be higher than those of unsuitable eggs. To reduce the production cost, LED lights and photodiodes were used as the light source and light receptors, respectively. A discrimination rate of 92.9% was obtained when LED was used as the light source. © 2015 IAgrE. Source
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