Kagawa Prefectural College of Health Sciences

www.pref.kagawa.jp/daigaku/
Takamatsu-shi, Japan

Kagawa Prefectural College of Health science is a public university in Takamatsu, Kagawa, Japan. The predecessor of the school was founded in 1999, and it was chartered as a university in 2004. Wikipedia.

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Nakamura T.,Japan Beekeeping Co. | Ohta Y.,Aichi University | Ohashi K.,Health Science University | Ikeno K.,Japan Beekeeping Co. | And 3 more authors.
Phytotherapy Research | Year: 2012

In the present study we examined the protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress (WIRS) in comparison with that of vitamin E (VE). Fasted rats orally received Brazilian green propolis ethanol extract (BPEE; 10, 50 or 100 mg/kg), VE (250 mg/kg) or vehicle at 30 min before the onset of WIRS. Exposure of vehicle-treated rats to 6 h of WIRS caused liver cell damage, judging from the levels of serum alanine aminotransferase and aspartate aminotransferease, increased hepatic lipid peroxide, NOx contents and myeloperoxidase activity, and decreased hepatic non-protein SH, ascorbic acid contents and superoxide dismutase activity. Preadministration of BPEE (50 or 100 mg/kg) or VE to the stressed rats protected against the hepatic damage and attenuated the increased hepatic lipid peroxide and NOx contents and myeloperoxidase activity and the decreased hepatic non-protein SH and ascorbic acid contents and superoxide dismutase activity. These protective effects of BPEE (50 mg/kg) were greater than those of BPEE (100 mg/kg) and were almost equal to those of VE. These results indicate that BPEE protects against hepatic oxidative damage in rats exposed to WIRS possibly through its antioxidant and antiinflammatory properties such as VE. Copyright © 2012 John Wiley & Sons, Ltd.


Hata T.,Kagawa University | Tanaka H.,Kagawa University | Noguchi J.,Kagawa Prefectural College of Health Sciences | Hata K.,Kagawa Prefectural College of Health Sciences
Placenta | Year: 2011

Conventional two-dimensional (2D) ultrasound has been widely used for the evaluation of the placenta during pregnancy. This 2D ultrasound evaluation includes the morphology, anatomy, location, implantation, anomaly, size, and color/power and pulsed Doppler sonographic assessment of the placenta. The introduction of three-dimensional (3D) ultrasound would facilitate the novel assessment of the placenta, such as surface-rendered imaging and volume measurement. With the recent advances in 3D power Doppler (3DPD) ultrasound as well as quantitative 3DPD histogram analysis, quantitative and qualitative assessments of the vascularization and blood flow of the placenta have become feasible. These novel techniques may assist in the evaluation of the feto-placental function, and offer potential advantages relative to conventional 2D sonographic assessments. 3D ultrasound may be an important modality in future placental research, in the evaluation of feto-placental insufficiency in clinical practice, and in the prediction of fetal growth restriction and pre-eclampsia, although some limitations regarding the assessment of the placenta employing 3D ultrasound still remain unresolved. © 2010 Elsevier Ltd. All rights reserved.


Sato M.,Kagawa University | Kanenishi K.,Kagawa University | Hanaoka U.,Kagawa University | Noguchi J.,Kagawa Prefectural College of Health Sciences | And 2 more authors.
International Journal of Gynecology and Obstetrics | Year: 2014

Objective To assess the frequency of fetal facial expressions at 20-24 weeks of gestation using four-dimensional (4D) ultrasonography and to determine whether there was any correlation between facial expression and gestational age (20-34 weeks). Methods The facial expressions of 23 healthy fetuses were examined using 4D ultrasound at 20-24 weeks. Each fetus was recorded continuously for 15 minutes. The frequencies of mouthing, yawning, smiling, tongue expulsion, scowling, sucking, and blinking were assessed and the data combined with those expressions observed at 25-34 weeks of gestation in two previous studies (n = 34) to determine the correlation between gestational age and each of the facial expressions. Results Mouthing was significantly more frequent than the other six facial expressions at 20-24 weeks (P < 0.05). Yawning was significantly more frequent than smiling, scowling, and blinking (P < 0.05), and sucking was significantly more frequent than smiling, scowling, and blinking (P < 0.05). The frequency of yawning, smiling, tongue expulsion, scowling, and blinking increased with gestational age (P < 0.05). Mouthing movement and sucking frequencies remained constant between 20 and 34 weeks. Conclusion Frequencies of complicated facial expressions such as smiling and scowling may increase with advancing gestation owing to the development of the fetal brain and central nervous system. © 2014 International Federation of Gynecology and Obstetrics.


Ohsaki H.,Ehime Prefectural University of Health Sciences | Hirakawa E.,Kagawa Prefectural College of Health Sciences | Nakamura M.,National Hospital Organization Zentsuji Hospital | Norimatsu Y.,Ehime Prefectural University of Health Sciences | And 2 more authors.
Cytopathology | Year: 2011

Objective: Reactive renal tubular cells show features of an atypical repair reaction. Differentiation between reactive renal tubular cells and low-grade urothelial carcinoma (LG-UC) cells can therefore be a diagnostic challenge based on morphology alone. In this study, we evaluated the diagnostic utility of vimentin and a high-molecular-weight cytokeratin antibody (clone 34ßE12) in differentiating reactive renal tubular cells from LG-UC. Methods: We evaluated voided urine cytology and surgical specimens from 40 patients with renal disease, and 17 patients with LG-UC. All slides were stained with vimentin and 34ßE12. Results: In the reactive renal tubular cells in voided urine cytology, vimentin showed strong cytoplasmic staining in 39/40 (97.5%) cases, but all were negative for 34ßE12. LG-UC cells showed positive staining for 34ßE12 in 3/17 (17.6%) cases, whereas none were positivity for vimentin. The reactive renal tubular cells of histological specimens in the renal disease group demonstrated positive for vimentin in all 40 cases and all were negative for 34ßE12. The LG-UC group showed abnormal staining for 34ßE12 in 4/17 (23.5%) cases, whereas none were positive for vimentin. Conclusions: Vimentin expression in urine cytology can help to distinguish reactive renal tubular cells from LG-UC. However, 34ßE12 does not appear to be a useful adjunct to distinguish these two groups in voided urine cytology. © 2010 Blackwell Publishing Ltd.


Kanenishi K.,Kagawa University | Hanaoka U.,Kagawa University | Noguchi J.,Kagawa Prefectural College of Health Sciences | Marumo G.,Itabashi Chuo Medical Center | Hata T.,Kagawa University
International Journal of Gynecology and Obstetrics | Year: 2013

Objective To assess the frequency of fetal facial expressions at 25-27 weeks of gestation using 4D ultrasound. Methods Twenty-four normal fetuses were examined using 4D ultrasound. The face of each fetus was recorded continuously for 15 minutes. The frequencies of tongue expulsion, yawning, sucking, mouthing, blinking, scowling, and smiling were assessed and compared with those observed at 28-34 weeks of gestation in a previous study. Results Mouthing was the most common facial expression at 25-27 weeks of gestation; the frequency of mouthing was significantly higher than that of the other 6 facial expressions (P < 0.05). Yawning was significantly more frequent than the other facial expressions, apart from mouthing (P < 0.05). The frequencies of yawning, smiling, tongue expulsion, sucking, and blinking differed significantly between 25-27 and 28-34 weeks (P < 0.05). Conclusion The results indicate that facial expressions can be used as an indicator of normal fetal neurologic development from the second to the third trimester. 4D ultrasound may be a valuable tool for assessing fetal neurobehavioral development during gestation. © 2013 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.


Noguchi J.,Kagawa University | Noguchi J.,Kagawa Prefectural College of Health Sciences | Tanaka H.,Kagawa University | Koyanagi A.,Miyake Clinic | And 2 more authors.
Archives of Gynecology and Obstetrics | Year: 2015

Background: We assessed placental perfusion based on placental vascular sonobiopsy (PVS) at 18–22 weeks of gestation in a low-risk population to predict fetal growth restriction (FGR) or pregnancy-induced hypertension [PIH; gestational hypertension (GH) and preeclampsia (PE)]. Methods: PVS using three-dimensional (3D) power Doppler ultrasound with the VOCAL imaging analysis program was performed in 226 pregnancies [FGR, 25; appropriate-for-gestational age (AGA) 191; and large-for-gestational age (LGA), 10] [PIH, 13 (GH, 7 and PE, 6) and non-PIH, 213] at 18–22 weeks of gestation. 3D power Doppler indices such as the vascularization index (VI), flow index (FI), and vascularization flow index (VFI) using PVS were calculated in each placenta. Results: There were no significant differences in VI, FI, or VFI values among FGR, AGA, and LGA pregnancies. No significant differences in VI, FI, or VFI values between PHI and non-PIH pregnancies were noted. There were also no significant differences in VI, FI, or VFI values between GH and PE pregnancies. Conclusions: 3D power Doppler placental vascular indices at 18–22 weeks could not be used to predict high-risk pregnancies that develop FGR or PIH in a low-risk population. © 2014, Springer-Verlag Berlin Heidelberg.


Itoh Y.,Asahikawa University | Ichihara K.,Yamaguchi University | Kishi K.,Sysmex Corporation Kobe | Hosogaya S.,Kagawa Prefectural College of Health Sciences | Yamada T.,Jichi Medical University
Clinica Chimica Acta | Year: 2012

Background: International external quality assessments have shown variation in results of urinary albumin among various immunoassays. A well-defined candidate reference material for urine albumin (cRM-UA) was prepared to improve standardization. Methods: cRM-UA was prepared from a commercially available preparation of human serum albumin by using gel-filtration HPLC. The value was assigned by transfer from ERM-DA470 using immunoassay systems qualified based on the linearity and variability observed in dilution tests of pooled urine and the calibrators. Effectiveness of recalibration using the cRM-UA was evaluated by measuring 129 urine specimens. Results: The cRM-UA had a monomeric albumin peak which accounted for 98.9% of the total area by gel filtration HPLC. The lyophilized preparation of the cRM-UA had suitable homogeneity, and short- and long-term stability. Nine of 14 immunoassays met the criteria were used for value assignment. The assigned concentration was 225.1 ± 9.11 mg/l [mean ± U: expanded uncertainty with k = 2] when reconstituted with 3.00. ml of purified water on weight basis. Recalibration of 7 qualified immunoassays using the cRM-UA resulted in between-method CV of 6.6%. Conclusions: The cRM-UA was successful in achieving standardization of urine albumin results among 7 immunoassays which possess performance attributes representing uniform reactivity to both cRM-UA and clinical urine samples. © 2011 Elsevier B.V.


Miyoshi M.,Kobe University | Miyoshi M.,Kagawa Prefectural College of Health Sciences | Sakaki H.,Kobe University | Sakaki H.,Tokushima Prefectural Central Hospital | And 5 more authors.
Clinical Nutrition | Year: 2011

Background & aims: Short-chain fatty acids, especially butyrate, have various biological activities including inhibition of tumor necrosis factor (TNF)-α secretion, via attenuation of nuclear factor-κB (NF-κB) activation. Here, we evaluated the protective effect of oral administration of tributyrin, a prodrug of butyrate, on lipopolysaccharide (LPS)-induced liver injury in rats. Methods: Rats were divided into four groups: normal control, tributyrin, LPS, and tributyrin/LPS (treated with tributyrin 1 h before LPS). Plasma levels of butyrate and TNF-α, expression of TNF-α, NF-κB, Toll-like receptor (TLR) 2, and TLR4 mRNA in liver, blood biochemical tests, and histopathological analysis of liver were performed. Results: Oral tributyrin increased plasma butyrate level in the portal vein to 2.4 mM at 1 h and 0.7 mM at 2.5 h. Tributyrin attenuated NF-κB activation and liver tissue injury associated with LPS injection. The increases in TNF-α level, and hepatic TLR2 mRNA expression were lower in the tributyrin/LPS group. We believe that this study provides the first evidence that orally administered tributyrin increases butyrate level in the hepato-portal system and attenuates liver injury and subsequent inflammatory responses. Conclusion: Oral tributyrin increased plasma butyrate in the portal vein and attenuated liver injury in endotoxemic rats. © 2010 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism.


Yamaza H.,Nagasaki University | Yamaza H.,Kyushu University | Komatsu T.,Nagasaki University | Wakita S.,Nagasaki University | And 8 more authors.
Aging Cell | Year: 2010

The FoxO transcription factors may be involved in the antiaging effect of calorie restriction (CR) in mammals. To test the hypothesis, we used FoxO1 knockout heterozygotic (HT) mice, in which the FoxO1 mRNA level was reduced by 50%, or less, of that in wild-type (WT) mouse tissues. The WT and HT mice were fed ad libitum (AL) or 30% CR diets from 12 weeks of age. Aging- and CR-related changes in body weight, food intake, blood glucose, and insulin concentrations were similar between the WT and HT mice in the lifespan study. The response to oxidative stress, induced by intraperitoneal injection of 3-nitropropionic acid (3-NPA), was evaluated in the liver and hippocampus at 6 months of age. Several of the selected FoxO1-target genes for cell cycle arrest, DNA repair, apoptosis, and stress resistance were up-regulated in the WT-CR tissues after 3-NPA injection, while the effect was mostly diminished in the HT-CR tissues. Of these gene products, we focused on the nuclear p21 protein level in the liver and confirmed its up-regulation only in the WT-CR mice in response to oxidative stress. The lifespan did not differ significantly between the WT and HT mice in AL or CR conditions. However, the antineoplastic effect of CR, as indicated by reduced incidence of tumors at death in the WT-CR mice, was mostly abrogated in the HT-CR mice. The present results suggest a role for FoxO1 in the antineoplastic effect of CR through the induction of genes responsible for protection against oxidative and genotoxic stress. © 2010 The Authors Journal compilation © Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland 2010.


Kabuto H.,Kagawa Prefectural College of Health Sciences | Yamanushi T.T.,Kagawa Prefectural College of Health Sciences
Neurochemical Research | Year: 2011

Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the nigrostriatal system and dopamine (DA) depletion in the striatum. The most popular therapeutic medicine for treating PD, 3-(3,4-Dihydroxyphenyl)-l-alanine (L-DOPA), has adverse effects, such as dyskinesia and disease acceleration. As superoxide (•O2 -) and hydroxyl radical (•OH) have been implicated in the pathogenesis of PD, free radical scavenging and antioxidants have attracted attention as agents to prevent disease progression. Rodents injected with 6-hydroxydopamine (6-OHDA) intracerebroventricularly are considered to be a good animal model of PD. Zingerone and eugenol, essential oils extracted from ginger and cloves, are known to have free radical scavenging and antioxidant effects. Therefore, we examined the effects of zingerone and eugenol on the behavioral problems in mouse model and on the DA concentration and antioxidant activities in the striatum after 6-OHDA administration and L-DOPA treatment. Daily oral administration of eugenol/zingerone and injection of L-DOPA intraperitoneally for 4 weeks following a single 6-OHDA injection did not improve abnormal behaviors induced by L-DOPA treatment. 6-OHDA reduced the DA level in the striatum; surprisingly, zingerone and eugenol enhanced the reduction of striatal DA and its metabolites. Zingerone decreased catalase activity, and increased glutathione peroxidase activity and the oxidized L-ascorbate level in the striatum. We previously reported that pre-treatment with zingerone or eugenol prevents 6-OHDA-induced DA depression by preventing lipid peroxidation. However, the present study shows that post-treatment with these substances enhanced the DA decrease. These substances had adverse effects dependent on the time of administration relative to model PD onset. These results suggest that we should be wary of ingesting these spice elements after the onset of PD symptoms. © 2011 Springer Science+Business Media, LLC.

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