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Ōsaka, Japan

Soga M.,Shionogi and Co. | Hashimoto S.,Shionogi and Co. | Kishimoto Y.,Shionogi and Co. | Hirasawa T.,Shionogi and Co. | And 3 more authors.
Experimental Animals | Year: 2010

In order to examine the influence of obesity on metabolic disorder and liver pathogenesis of the Fatty Liver Shionogi (FLS) mouse, which develops hereditary fatty liver and spontaneous liver tumors, we established a new congenic strain named FLS-Lepob. The Lepob gene of the C57BL/6JWakShi (B6)-Lepob/Lepob mouse was transferred into the genome of the FLS mouse, by backcross mating. FLS-Lepob/Lepob mice were maintained by intercrossing between Lepob-heterozygous littermates. The FLS-Lepob/Lepob mice of both sexes developed remarkable hyperphagia, obesity and type 2 diabetes mellitus. At 12 weeks of age, glucosuria was detected in all male and female FLS-Lepob/Lepob mice. Biochemical examination demonstrated that the FLS-Lepob/Lepob mice have severe hyperlipidemia and hyperinsulinemia. The livers of FLS-Lepob/Lepob mice showed microvesicular steatosis and deposition of large lipid droplets in hepatocytes throughout the lobules. The steatohepatitis-like lesions including the multifocal mononuclear cell infiltration and clusters of foamy cells were observed earlier in FLS-Lepob/Lepob mice than in FLS mice. B6-Lepob/Lepob mice did not show hepatic inflammatory change. Furthermore, FLS-Lepob/Lepob mice developed multiple hepatic tumors including hepatocellular adenomas and carcinomas following steatohepatitis. In conclusion, the FLS-Lepob/Lepob mice developed steatohepatitis and hepatic tumors following hepatic steatosis. The FLS-Lepob/Lepob mouse with obesity and type 2 diabetes mellitus might be a useful animal model for human non-alcoholic steatohepatitis (NASH).

Ochi T.,Astellas Research Technologies Co. | Nishiura I.,Astellas Research Technologies Co. | Tatsumi M.,Astellas Research Technologies Co. | Hirano Y.,Astellas Research Technologies Co. | And 13 more authors.
Experimental Animals | Year: 2013

Here, to determine the effects of transport stress on blood parameters in dogs, we investigated the changes in hematologic and serum chemical parameters in healthy beagle dogs transported from Beijing, China, to Osaka, Japan, to obtain the background data. Only the activity of serum alkaline phosphatase increased clearly upon arrival, a change attributed to transport stress, but the activity gradually reduced afterward. No marked changes in levels of other blood parameters were noted. Our findings here suggest that alkaline phosphatase is a useful tool for studying transport stress. Key words: alkaline phosphatase, blood parameter, dog, transport stress. © 2013 Japanese association for Laboratory animal science.

Koga T.,Daiichi Sankyo | Aoki W.,KAC Co. | Mizuno T.,KAC Co. | Wakazono K.,KAC Co. | And 8 more authors.
Journal of Microbiology, Immunology and Infection | Year: 2015

Background: Campylobacter spp. are zoonotic pathogens, however, knowledge about their presence and antimicrobial resistance in nonhuman primates is limited. Our animal facility purchased cynomolgus monkeys (Macaca fascicularis) from various Asian countries: China, Cambodia, Indonesia, the Philippines, and Vietnam. Methods: Colonization by Campylobacter spp. was investigated in 238 of the monkeys from 2009 to 2012 and antimicrobial susceptibility testing was carried out for these isolates. Furthermore, we eradicated these pathogens from these monkeys. Results: Campylobacter spp. were isolated from 47 monkeys from three specific countries: China, Cambodia, and Indonesia, with respective isolation rates of 15%, 36%, and 67%. Two monkeys, which were each infected with Campylobacter jejuni and Campylobacter coli, showed clinical symptoms of diarrhea and bloody feces. In total, 41 isolates of C. coli and 17 isolates of C. jejuni were detected. Antimicrobial susceptibility varied: in the monkeys from China, erythromycin (ERY)-, tetracycline (TET)-, and ciprofloxacin-resistant C. coli, in the monkeys from Cambodia, amoxicillin-intermediate, TET- and ciprofloxacin-resistant C. coli and amoxicillin- and ciprofloxacin-resistant C. jejuni, and in the monkeys from Indonesia, ciprofloxacin-resistant C. coli and TET- and ciprofloxacin-resistant C. jejuni were common (>75%). Multiresistant isolates of C. coli were found in monkeys from all countries and multiresistant isolates of C. jejuni were found in monkeys from Indonesia. The eradication rate with azithromycin was comparable to that with gentamicin (GEN) by oral administration, and was higher than those with amoxicillin-clavulanic acid (AMC) and chloramphenicol (CHL). Conclusion: From the perspective of zoonosis, we should acknowledge multiresistant Campylobacter spp. isolated from the monkeys as a serious warning. © 2015.

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