K2R Inc.

Kitakyūshū, Japan
Kitakyūshū, Japan
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Kawano T.,University of Kitakyushu | Kawano T.,University of Florence | Kawano T.,University Paris Diderot | Kagenishi T.,University of Kitakyushu | And 31 more authors.
Communicative and Integrative Biology | Year: 2015

Generation of reactive oxygen species is useful for various medical, engineering and agricultural purposes. These include clinical modulation of immunological mechanism, enhanced degradation of organic compounds released to the environments, removal of microorganisms for the hygienic purpose, and agricultural pest control; both directly acting against pathogenic microorganisms and indirectly via stimulation of plant defense mechanism represented by systemic acquired resistance and hypersensitive response. By aiming to develop a novel classes of artificial redox-active biocatalysts involved in production and/or removal of superoxide anion radicals, recent attempts for understanding and modification of natural catalytic proteins and functional DNA sequences of mammalian and plant origins are covered in this review article. © Tomonori Kawano, Tomoko Kagenishi, Takashi Kadono, François Bouteau, Takuya Hiramatsu, Cun Lin, Kenichiro Tanaka, Licca Tanaka, Stefano Mancuso, Kazuya Uezu, Tadashi Okobira, Hiroka Furukawa, Junichiro Iwase, Reina Inokuchi, Frantisek Baluška, and Ken Yokawa.

Iwase J.,University of Kitakyushu | Furukawa H.,University of Kitakyushu | Hiramatsu T.,University of Kitakyushu | Bouteau F.,University of Kitakyushu | And 11 more authors.
Journal of Experimental Botany | Year: 2014

The impact of copper ions on the oxidative and calcium signal transductions, leading to cell death in plant cells, have been documented. Copper induces a series of biological and chemical reactions in plant cells including the oxidative burst reflecting the production of reactive oxygen species and the stimulation of calcium channel opening allowing a transient increase in cytosolic calcium concentrations. These early events, completed within a few minutes after the contact with copper, are known to trigger the development of cell death. The effects of DNA fragments with copper-binding motifs as novel plant cell-protecting agents were assessed using cell suspension cultures of transgenic tobacco (Nicotiana tabacum L., cell line BY-2) expressing the aequorin gene. The addition of GC-rich double-stranded DNA fragments, prior to the addition of copper ions, effectively blocked both the copper-induced calcium influx and cell death. In addition, the DNA-Cu complex examined was shown to possess superoxide-scavenging catalytic activity, suggesting that DNA-mediated protection of the cells from copper toxicity is due to the removal of superoxide. Lastly, a possible mechanism of DNA-Cu interaction and future applications of these DNA fragments in the protection of plant roots from metal toxicity or in aid of phyto-remediation processes are discussed. © The Author 2014.

Provided is a method for manufacturing a titania coated alumina fiber aggregate which includes the steps of: forming an aluminum fiber aggregate where aluminum fibers are aggregated with density per unit volume of 0.5 g/cm^(3 )to 3 g/cm^(3); forming an alumina fiber aggregate where an oxide film having a film thickness of 50 nm or more is formed on alumina fibers; and forming a titania coated alumina fiber aggregate where a titania thin film is formed on alumina fibers.

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