Baraut, India
Baraut, India

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Kumar S.,Punjabi University | Kumar S.,JV College | Verma N.,Punjabi University | Singh A.K.,Punjabi University
Sensors and Actuators, B: Chemical | Year: 2017

A novel and highly specific recombinant biosensor has been investigated for detection of highly toxic metal ion cadmium (Cd(II)) based on Escherichia coli DH5α (pNV12). The working principle of biosensor is based on the expression of gfp gene under the control of cad promoter and the cadC gene of Staphylococcus aureus plasmid pI258. Escherichia coli DH5α (pNV12) was tested for its response in the presence of different combinations of Pb(II), Cd(II) and Zn(II) ions. The investigation resulted in the development of a cadmium specific recombinant whole cell biosensor with a response time as low as 15 min. A good linear range for cadmium ion concentrations of 10–50 μg/l with R2 0.9946 was obtained and the detection limit of 10 μg/l. Further, we reduced the detection limit up to 5 μg/l by increasing the incubation time from 15 to 30 min. High throughput microarray techniques for testing of 48 samples simultaneously has also been developed with reaction volume miniaturized from 2000 μl to 200 μl. The developed biosensor was applied to detect cadmium ion concentration in milk samples collected from different areas of Punjab, India. Two samples collected from industrial areas, shown to have cadmium concentration above permissible limits (10 μg/l). © 2016


Kumar V.,Jv College | Jain G.,Jv College | Kishor S.,Jv College | Ramaniah L.M.,Bhabha Atomic Research Center
Computational and Theoretical Chemistry | Year: 2011

The structures and energetics, as well as several important chemical parameters, of some alkylating drug molecules in both neutral and charged states, are investigated. The reactivity of the drug molecules with the DNA bases is then determined. These studies are carried out within the framework of first-principles density functional theory (DFT), using the Becke-Lee-Yang-Parr (BLYP) generalized gradient corrections to the local spin density approximation exchange and correlation energy, norm-conserving pseudopotentials, a plane-wave expansion of Kohn-Sham orbitals, and conceptual DFT. Our results are in good agreement with the few existing experiments. Further, for most of the drug molecules studied, the Guanine moiety in DNA is found to be the primary target, validating earlier experimental findings for anti-tumour drugs. © 2011 Elsevier B.V.


Kumar N.,Indian Central Electronics Engineering Research Institute | Kumar N.,JV College | Singh U.,Indian Central Electronics Engineering Research Institute | Kumar A.,Indian Central Electronics Engineering Research Institute | And 3 more authors.
Journal of Fusion Energy | Year: 2010

This paper presents the design of 127.5 GHz ITER start-up gyrotron interaction cavity. Particle-in-Cell electromagnetic simulation approach has been used for the cold cavity and beam-wave interaction analysis. In-house developed code GCOMS has been used for the mode selection. TE24,8 mode has been chosen as the operating mode. The simulation results show the output power more than 1.5 MW at the operating frequency of 127.8 GHz and the cavity centre magnetic field of 5.1 T. The study of the parametric dependency of the output power and the efficiency on the electron beam and the cavity geometry parameters has also been carried out. © 2010 Springer Science+Business Media, LLC.


Kumar N.,Indian Central Electronics Engineering Research Institute | Alaria M.K.,Indian Central Electronics Engineering Research Institute | Singh U.,Indian Central Electronics Engineering Research Institute | Bera A.,Indian Central Electronics Engineering Research Institute | And 2 more authors.
Journal of Infrared, Millimeter, and Terahertz Waves | Year: 2010

A beam tunnel for a 42 GHz, 200 kW gyrotron for an Indian TOKAMAK system has been designed. The initial design of the beam tunnel has been carried out on the basis of the required electron beam parameters at the interaction cavity and the electron beam simulation of the magnetron injection gun. The design optimization of the beam tunnel has been done with the help of 3-D simulation software CST-Microwave Studio. In the simulation, the absorption, the reflection and the transmission of RF power by the beam tunnel have been analyzed. Three different lossy ceramics, Al 2O 3-SiC, AlN-SiC and BeO-SiC have been investigated during the simulation. The simulation results obtained with CST-Microwave Studio have been validated with another 3-D simulation software HFSS. The Q value of the beam tunnel for different ceramic material has also been analyzed to investigate the parasitic mode excitation in the beam tunnel. © 2010 Springer Science+Business Media, LLC.


Kumar N.,Indian Central Electronics Engineering Research Institute | Kumar N.,JV College | Singh U.,Indian Central Electronics Engineering Research Institute | Singh T.P.,JV College | Sinha A.K.,Indian Central Electronics Engineering Research Institute
Physics of Plasmas | Year: 2011

This paper presents the design criteria of the parasitic mode oscillations suppression for a periodic, ceramic, and copper loaded gyrotron beam tunnel. In such a type of beam tunnel, the suppression of parasitic mode oscillations is an important design problem. A method of beam-wave coupling coefficient and its mathematical formulation are presented. The developed design criteria are used in the beam tunnel design of a 42 GHz gyrotron to be developed for the Indian TOKAMAK system. The role of the thickness and the radius of the beam tunnel copper rings to obtain the developed design criteria are also discussed. The commercially available electromagnetic code CST and the electron trajectory code EGUN are used for the simulations. © 2011 American Institute of Physics.


Kumar N.,Indian Central Electronics Engineering Research Institute | Kumar N.,Jv College | Kumar A.,Indian Central Electronics Engineering Research Institute | Singh U.,Indian Central Electronics Engineering Research Institute | And 3 more authors.
International Journal of Thermophysics | Year: 2011

Thermal and structural analysis for a 120 GHz, 1 MW gyrotron interaction cavity and the effect of structural deformation on the operational mode excitation is presented. Finite element analysis codes ANSYS and COSMOS have been used for the thermal and structural simulation. The values of the water flow rate and the hydraulic diameter of the outer jacket have been optimized. The effect of the maximum radial expansion of the interaction cavity on eigenfrequency and mode excitation has also been analyzed. The change in eigenfrequency for the operating mode due to thermal expansion is 0.606GHz. This value is very near the theoretically calculated value and under the tolerance limit of the maximum frequency deviation for the gyrotron operating mode. © Springer Science+Business Media, LLC 2011.


Kumar N.,Indian Central Electronics Engineering Research Institute | Kumar N.,JV College | Singh U.,Indian Central Electronics Engineering Research Institute | Kumar A.,Indian Central Electronics Engineering Research Institute | And 3 more authors.
Progress In Electromagnetics Research B | Year: 2011

The complete design of 35GHz, 200kW gyrotron for various material processing and heating applications is presented in this article. The components of the device, such as Magnetron Injection Gun, interaction cavity, collector and RF window, are designed for the TE03 mode. Various in-house developed codes (GCOMS, MIGSYN and MIGANS) and commercially available codes (MAGIC, EGUN and CST-MS) are used for the design purpose. A thorough sensitivity analysis of the gyrotron components is also carried out. The designed device shows the capability to generate more than 200kW of output power with more than 40% of efficiency.


Kumar N.,Indian Central Electronics Engineering Research Institute | Kumar N.,JV College | Singh U.,Indian Central Electronics Engineering Research Institute | Singh T.P.,JV College | Sinha A.K.,Indian Central Electronics Engineering Research Institute
International Journal of Electronics | Year: 2011

Parasitic oscillation excitation analysis has been carried out for the 42 GHz gyrotron beam tunnel. This article presents a systematic approach for the analysis of parasitic oscillation excitation. The electron trajectory code EGUN has been used for the estimation of the electron beam parameters in the beam tunnel. The electromagnetic simulation code CST-MS has been used for the eigenmode and Q value analysis. The analysis of the parasitic oscillations has been performed for the symmetric TE modes and the first three cavity side copper rings. Four different approaches- the Q value study, the mode maxima-electron beam radius mismatching, the electron cyclotron frequency-mode excitation frequency mismatching and the backward wave interaction analysis- have been used for the parasitic oscillation analysis. © 2011 Taylor & Francis.


Different Schiffbases derived by nucleophilic addition of (i) 4-amino-3-ethyl-5-mercapto-s-triazole and 4-amino-5-mercapto-3-n-propyl-s-triazole to p-chlorobenzaldehyde, 2-acetyl pyridine, 4-methyl acetophenone, 2,5-dinitroacetophenone (ii) 4-amino-5-mercapto-3-trifluoromethyl-s-triazole to p-chlorobenzaldehyde and 4-methylacetophenone and their metal complexes of Ni(ii), Cu(ii), have been synthesized and characterized by elemental analysis, IR, PMR, Electronic magnetic and conductance measurement.


Schiffbases 5-phenylazo salicylidene-3-Hydrazino-5,6-Diphenyl-1,2, 4-Triazine was prepared by the dissolving of 3-Hydrazino-5,6-diphenyl-1,2,4-triazine dropwise to the ethanolic solution of 5-phenylazo salicylaldehyde (PAS). The ligand and Cu(II), Co(II), Zn(II), Cd(II), Hg(II) metal complexes were screened against selected fungi and bacteria to examine their antimicrobial properties. gram positive bacteria (Bacillus substilis and Staphylococcus aureus) and gram negative bacteria (Escherichia coli, Klebsiella pneumonia, Pseudomonos aerogens, Salmonella paratyphi B and Proteus vulgaris).

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