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Song E.J.,Pukyong National University | Kim A.R.,Ju Yeong NS Co. | Kim K.B.W.R.,Pukyong National University | Lee S.Y.,Korea Food Research Institute | And 6 more authors.
Food Science and Biotechnology | Year: 2011

Ecklonia cava (EC) was irradiated with γ-rays at doses of 3, 7, and 20 kGy. The extraction yields, total polyphenol content (TPC), DPPH radical scavenging activity, antimicrobial activity, and color of the EC extracts were assessed. The results showed that irradiation caused an increase in the extraction yields of ethanol and water. TPC was found to be significantly increased when EC powder was subjected to irradiation, followed by ethanol and water extraction. However, DPPH radical scavenging and antimicrobial activities were stable under all irradiation conditions. In the heat and pH stability tests, the DPPH radical scavenging activities of EC ethanol extracts were not influenced by irradiation. Irradiation caused an increase in the lightness and redness of EC extracts and decreased the yellowness. In conclusion, γ-irradiation increased the extraction yield and TPC and brightened the color, while maintaining the antioxidant and antimicrobial activities. © KoSFoST and Springer 2011.

Lee D.-G.,Kyung Hee University | Park J.-H.,Kyung Hee University | Yun J.-H.,Ju Yeong NS Co. | Song M.-C.,Kyung Hee University | And 3 more authors.
Food Science and Biotechnology | Year: 2011

Typical solvent extraction of succinic acid from a chitosan-succinic acid powder mixture gave unsatisfactory yield (10. 0%) due to the presence of intramolecular crosslinks between the chitosan amine groups and succinic acid carboxylic groups. An improved extraction method, which included hydrolysis of cross-links by KOH to liberate succinic acid from the matrix, afforded satisfactory yield (95. 2±4. 0%). Quantitative analysis of succinic acid in chitosan-succinic acid mixture was performed using GCMS and the optimized analysis method showed good validation in terms of specificity, linearity, accuracy (recovery rate), precision (inter-day and intra-day precision), limit of quantification (LOQ), and limit of detection (LOD). © 2011 The Korean Society of Food Science and Technology and Springer Netherlands.

Sim B.Y.,Daejeon University | Bak J.W.,Daejeon University | Lee H.J.,Daejeon University | Jun J.A.,Daejeon University | And 6 more authors.
Journal of Nutrition and Health | Year: 2015

Purpose: The aim of this study is to investigate anti-arthritis activity using natural eggshell membrane (NEM). Methods: NEM was administered at 52 mg/kg, 200 mg/kg, and 400 mg/kg to SD-Rat, where arthritis was induced by monosodium iodoacetate (MIA) at 3 mg. NO production in serum was measured using Griess reagent. Cytokines including IL-1β, and IL-6 were measured by Luminex and PGE2, MMP-2, MMP-9, TIMP-1, LTB4, and hs-CRP were measured by ELISA. The cartilage of patella volume was examined and 3-D high-resolution reconstructions of the cartilage of patella were obtained using a Micro-CT system. Results: Production of NO, IL-1β, IL-6, PGE2, MMP-2, MMP-9, TIMP-1, LTB4, and hs-CRP in serum was decreased, respectively, in comparison with control. The cartilage of patella volume increased significantly. In addition, the NEM group showed a decrease in the cartilage of patella, synovial membrane, and transformation of fibrous tissue. Conclusion: The results for NEM showed significant anti-arthritis activity. These results may be developed as a raw material for new health food to ease the symptoms mentioned above. © 2015 The Korean Nutrition Society.

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