Vilnius, Lithuania
Vilnius, Lithuania

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Veteikyte A.,Vilnius University | Siekstele R.,Vilnius University | Tvaska B.,JSC Biocentras | Matijosyte I.,Vilnius University
Applied Microbiology and Biotechnology | Year: 2017

Currently, much attention is paid to technologies which can be drivers of the circular economy across different sectors, in particular, to develop technologies for utilization or reusability of biocompatible materials from industrial waste. One of such is the milk whey, which is a cheap biobased raw material, the disposal of which is a major problem for the dairy industry. Our proposed and investigated technology is based on a continuous exploitation of the whey combining microbiology and biotechnology. Primarily, whey was used as a nutrition source for the cultivation of Kluyveromyces lactis with the aim to produce the targeted biocatalyst—lipase. During cultivation, the whey was transformed into the hydrolyzed form, which was further successfully applied as a protein feeder (external linker) for immobilization of lipase by cross-linked enzyme aggregate (CLEA) method. The first time use of whey as a co-feeder for immobilization of enzymes by CLEA method has shown promising results and increased the stability of lipases for temperature and organic solvents. Hydrolysis of rapeseed oil catalyzed with immobilized derivatives was obtained with 45–96% efficiency at non-optimized conditions. Additionally, the determined kinetic parameters indicated that the rate of p-nitrophenyl palmitate hydrolysis was not changed drastically after immobilization. © 2017 Springer-Verlag Berlin Heidelberg


Aikaite-Stanaitiene J.,JSC Biocentras | Grigiskis S.,Vilnius Gediminas Technical University | Levisauskas D.,Kaunas University of Technology | Cipinyte V.,JSC Biocentras | And 2 more authors.
Journal of Environmental Engineering and Landscape Management | Year: 2010

A new composting technology of waste with high fat content was developed in JSC "Biocentras". The composting technology of fat-contaminated waste is based on the use of fat-oxidizing microorganisms. Developed technology is commended for cleaner production/pollution prevention approach as well as meets strict environmental and hygiene requirements. The composting process was investigated for the process optimization by applying the response surface methodology. Values of parameters of composting process were monitored in lab-scale composters. The optimal composition of the composing mixture was determined: the initial fat content - 5%, the concentration of bacterial preparation cells -109 CFU/g, the quantity of structural materials - 9.5%. Fat degradation rate slowed down 3 times if the initial fatty concentration increased from 5% to 20%. Concentrated fatty-waste disposal site prototype was designed. Composting process duration lasted 1 to 1.5 year.


Javmen A.,JSC Biocentras | Javmen A.,State Scientific Research Institute Center for Innovative Medicine | Grigiskis S.,JSC Biocentras | Rudenkov M.,Vilnius Gediminas Technical University | Mauricas M.,State Scientific Research Institute Center for Innovative Medicine
Protein Journal | Year: 2013

A β-1,3-endoglucanase produced by Streptomyces rutgersensis was purified to a homogeneity by the fractional precipitation with ammonium sulfate, ion exchange chromatography on Q-Sepharose and hydrophobic chromatography on Butyl Sepharose. A typical procedure provided 11.74-fold purification with 12.53 % yield. SDS-PAGE of the purified protein showed one protein band. The exact molecular mass of the enzyme obtained by mass spectrometry was 41.25 kDa; the isoelectric point was between pH 4.2-4.4. The optimal β-glucanase catalytic activity was at pH 7 and 50 C. An enzyme was only active toward glucose polymers containing β-1,3 linkages and hydrolyzed Saccharomyces cerevisiae cell wall β-glucan in an endo-like way: reaction products were different molecular size β-glucans, which were larger than glucose. © 2013 Springer Science+Business Media New York.


Matikeviciene V.,JSC Biocentras | Grigiskis S.,JSC Biocentras | Levisauskas D.,Kaunas University of Technology | Sirvydyte K.,JSC Biocentras | And 3 more authors.
Vide. Tehnologija. Resursi - Environment, Technology, Resources | Year: 2011

The aim of this study was to identify and optimize significant technological parameters influencing keratinolytic enzyme production by A. fradiae 119 and to study its ability to degrade keratin. In the present work chicken feathers meal (CFM) was found to be an excellent substrate for keratinase induction by A. fradiae 119. The strain produced 164 KU/mL keratinolytic activity in basal medium containing 7.5 g/L CFM as the sole source of carbon and nitrogen. Increased keratinolytic activity was achieved in media with ammonium sulfate as nitrogen source, the application of additional nitrogen sources to media containing CFM slightly decreased keratinase synthesis. Optimal parameters of the cultivation process were determined: pH of cultivation medium - 7.2, temperature - 34 °C and inoculum's size - 8%, using the response surface methodology. The yield of keratinase activity was increased by 46% (267 KU/mL) after optimization of the cultivation process. The good ability of cultural liquid to degrade feathers and wool was detected.


Javmen A.,State Scientific Research Institute Center for Innovative Medicine | Mauricas M.,State Scientific Research Institute Center for Innovative Medicine | Nemeikaite-Ceniene A.,State Scientific Research Institute Center for Innovative Medicine | Grigiskis S.,JSC Biocentras
International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings | Year: 2014

Nowadays non-cellulosic, β-glucans from the different source are intensively investigated due to the fact that these biological polymers are recognized as potent immunological stimulators for the mammal's immune system. Dendritic cells are the most important antigen presenting cells for activating naive T cells. The research goals of the recent investigation were to determine how the different molecular weight Saccharomyces cerevisiae β- glucan preparations affect such properties of the murine DC as phagocytosis, proliferation and cytokine synthesis in vitro. © 2015 IEEE.


Cipinyte V.,JSC Biocentras | Grigiskis S.,JSC Biocentras | Sapokaite D.,JSC Biocentras | Baskys E.,JSC Biocentras
Vide. Tehnologija. Resursi - Environment, Technology, Resources | Year: 2011

Different screening methods, such as emulsification capacity and oil spreading assays, hydrocarbon overlay agar and modified drop collapse methods were used to detect biosurfactant production by hydrocarbon degrading Arthrobacter sp N3 strain. It was indicated that oil spreading assay was the most reliable method to detect biosurfactant production. To investigate biosurfactant production, batch cultivation of Arthrobacter sp N3 was carried out in a fermenter with complex nutrient medium supplemented by sunflower oil as a carbon source. The highest oil displacement activity was achieved when Arthrobacter sp N3 strain was cultivated in two stages (with aeration for cell production and without aeration for biosurfactant synthesis). Then, two forms of the biosurfactant (crude preparation and partially purified biosurfactant) were recovered from the culture liquid. Furthermore, the biosurfactant produced by Arthrobacter sp N3 strain was analyzed by thin layer chromatography and it was estimated that even a few compounds have surface activity. The effect of temperature and pH on biosurfactant activity was also studied. It was observed that no appreciable changes in biosurfactant activity occurred at temperature and pH values ranges of 4-125 °C and 5-10, respectively.


Kleinaite E.,Vilnius University | Jaska V.,JSC Biocentras | Tvaska B.,JSC Biocentras | Matijosyte I.,Vilnius University
Journal of Cleaner Production | Year: 2014

By this study we aimed to develop a green and cleaner alternative to the existing oil-derived lubricant production by providing the market biolubricant synthesis catalyzed by enzymes. Moreover, the use of biodiesel to develop a higher added-value product is a very attractive solution for biodiesel production manufacturers in extending their production supply and the potential sales markets, especially in the East Europe region. The synthesis of 2-ethyl-1-hexyl oleate (biolubricant) was studied using commercially available immobilized lipase Lipozyme TL IM, biodiesel as a source of fatty acids and relatively cheap 2-ethyl-1-hexanol. The transesterification reaction was performed in solvent-free and water-free system. 2-Ethyl-1-hexyl oleate was successfully synthesized in 50 L scale with 100 % conversion in 10 h of reaction time at 60 °C temperature. The physico-chemical properties of 2-ethyl-1-hexyl oleate were estimated. © 2014 Elsevier Ltd. All rights reserved.


Javmen A.,JSC Biocentras | Grigiskis S.,JSC Biocentras | Rudenkov M.,JSC Biocentras | Pelisauskaite E.,JSC Biocentras
Minerva Biotecnologica | Year: 2013

Aim: β-glucan is polymeric compound in which glucose monomers are linked by β glycosidic bonds, β-glucan is produced by many different organisms - bacteria, fungi, plants. It was noted that different β-glucans can effect mammals immunity and can be human immune system modulators. Many experiments showed that β-glucans can be possibly used in bacterial disease therapy and can increase immune system cells cytotoxicity against cancer tumors. An important source of immune active β-glucan is baker's yeast Saccharomicies cerevisiae: one of the major and structurally important component of S. cerevisiae yeast cell wall component is β-(1-3, 1-6) glucan. For baker's yeast cultivation, YEPD medium is often used, which consists of glucose, yeast extract and peptone. The aim of this work was to use usual pabular low-cost glucose for Saccharomicies cerevisiae fermentations and optimizate cultivation process conditions for maximal β-glucan yield extraction from yeast cells. Methods: To reach this aim response surface methodology (RSM) was used. Alternate medium was also used, for cultivation of Saccharomicies cerevisiae, which consists of low-cost glucose and corn steep liquor; yeast cultivation process optimization for maximum yield of β-glucan was undertaken. Results: Optimal conditions were determined and verified for both two medium types. Conclusion: Both optimized mediums can be practical used for a β-glucan production.


Siekstele R.,Vilnius University | Veteikyte A.,Vilnius University | Tvaska B.,JSC Biocentras | Matijosyte I.,Vilnius University
Journal of Industrial Microbiology and Biotechnology | Year: 2015

Many microbial lipases have been successfully expressed in yeasts, but not in industrially attractive Kluyveromyces lactis, which among other benefits can be cultivated on a medium supplemented with whey––cheap and easily available industrial waste. A new bacterial lipase from Serratia sp. was isolated and for the first time expressed into the yeast Kluyveromyces lactis by heterologous protein expression system based on a strong promoter of Kluyveromyces marxianus triosephosphate isomerase gene and signal peptide of Kluyveromyces marxianus endopolygalacturonase gene. In addition, the bacterial lipase gene was synthesized de novo by taking into account a codon usage bias optimal for K. lactis and was expressed into the yeast K. lactis also. Both resulting strains were characterized by high output level of the target protein secreted extracellularly. Secreted lipases were characterized for activity and stability. © 2015, Society for Industrial Microbiology and Biotechnology.


PubMed | JSC Biocentras and Vilnius University
Type: Journal Article | Journal: Journal of industrial microbiology & biotechnology | Year: 2015

Many microbial lipases have been successfully expressed in yeasts, but not in industrially attractive Kluyveromyces lactis, which among other benefits can be cultivated on a medium supplemented with whey--cheap and easily available industrial waste. A new bacterial lipase from Serratia sp. was isolated and for the first time expressed into the yeast Kluyveromyces lactis by heterologous protein expression system based on a strong promoter of Kluyveromyces marxianus triosephosphate isomerase gene and signal peptide of Kluyveromyces marxianus endopolygalacturonase gene. In addition, the bacterial lipase gene was synthesized de novo by taking into account a codon usage bias optimal for K. lactis and was expressed into the yeast K. lactis also. Both resulting strains were characterized by high output level of the target protein secreted extracellularly. Secreted lipases were characterized for activity and stability.

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