Hitachi, Japan
Hitachi, Japan

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Sato H.,Chiba University | Funaki A.,Chiba University | Kimura Y.,Chiba University | Sumitomo M.,Chiba University | And 3 more authors.
Nutrition Research | Year: 2016

In this study, we examined the cell differentiation effect of an ethanol extract of Cyclolepis genistoides D. Don, a herbaceous perennial belonging to the family Asteraceae (vernacular name: palo azul). Palo azul has numerous physiological effects that contribute to the prevention of metabolic syndromes, although the mechanism remains unclear. We previously suggested that palo azul has antidiabetic activity via an adipose differentiation effect. Here, we focused on whether palo azul promoted the differentiation of myoblasts. The mouse muscle myoblast cell line C2C12 was cultured and differentiated using horse serum with or without an ethanol extract of palo azul (12.5-200 μg/mL). Quantitative real-time polymerase chain reaction was performed to evaluate differentiation markers, including insulin-like growth factor-1 and myogenin. To evaluate myotube formation, myosin heavy-chain (MHC) expression and localization were detected by immunohistochemistry. Palo azul increased the expression of the differentiation markers. Furthermore, immunohistochemistry analysis revealed increased formation of MHC myotubes after palo azul treatment along with increased diameter and fusion indices of the myotubes. The expression level of MHC was also increased. In conclusion, palo azul may increase muscle mass in the body and improve insulin resistance conditions by facilitating the formation of myotubes by promoting myocyte differentiation. © 2016 Elsevier Inc.


Sato H.,Chiba University | Ishikawa M.,Chiba University | Funaki A.,Chiba University | Kimura Y.,Chiba University | And 4 more authors.
Nutrition Research | Year: 2013

Cyclolepis genistoides D. Don is a herbaceous perennial belonging to the family Asteraceae, and its vernacular name is "palo azul" (palo). Palo has been reported to exhibit many physiological effects that contribute to the prevention of metabolic syndromes, although its mechanism is unclear. Among palo's various activities, we investigated the hypothesis that palo promotes adipocytes differentiation and regulates adipokine profiles in 3T3-L1 adipocytes by modulation of peroxisome proliferator-activated receptor (PPAR) γ, a major regulator of adipose differentiation. 3T3-L1 adipocytes were cultured and differentiated in Dulbecco modified Eagle medium with 50 to 200 μg/mL palo for 7 days or were cultured with palo without differentiation protocol for 14 days. Palo down-regulated the expression of 2 types of expressed/secreted adipokines, leptin and resistin, in a concentration-dependent manner. Under a nondifferentiated condition, palo promoted the accumulation of lipid droplets in cells. Real-time polymerase chain reaction and luciferase reporter assay showed that palo up-regulated expression and transcriptional activity of PPAR. γ. Furthermore, palo increased the expression of insulin-sensitizing adipokine, adiponectin, which is a directly target of PPAR. γ, both at the messenger RNA level and at the protein level. In summary, palo demonstrated the potential to improve insulin resistance by promoting adipocyte differentiation via PPAR. γ activation. Results suggest an increase in adiponectin secretion and a decrease in insulin-resistant factors such as leptin and resistin. © 2013 Elsevier Inc.


Mori C.,Chiba University | Kakuta K.,Kakuta Child and Allergy Clinic | Matsuno Y.,Chiba University | Todaka E.,Chiba University | And 4 more authors.
Environmental Science and Pollution Research | Year: 2014

Individuals' exposure to various persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs), and its adverse health effects have been a cause of concern. We measured blood PCB concentrations from samples taken from 507 Japanese individuals ranging from infants to those over 80 years of age. The blood PCB levels increased with age for both male (Spearman's r = 0.69, p < 0.001) and female (Spearman's r = 0.70, p < 0.001) participants. Adult men and nulliparous women showed similar increases with age. However, the PCB levels of multiparous women were lower than those of nulliparous women in their thirties (p = 0.005), probably because the PCBs were transferred from the mothers to their children during pregnancy and lactation. Among infants (<2 years of age), some had as high levels of accumulated PCB levels as those in adults >30 years of age. In some cases, the PCB levels were over 0.8 ng/g wet weight, similar to levels observed in adults over 50 years of age. In the future, it will be necessary to do research on the health of the children who are exposed by high concentration level of POPs. © 2013 The Author(s).


PubMed | JPD Co., Chiba University and IHM Inc
Type: Journal Article | Journal: Nutrition research (New York, N.Y.) | Year: 2016

In this study, we examined the cell differentiation effect of an ethanol extract of Cyclolepis genistoides D. Don, a herbaceous perennial belonging to the family Asteraceae (vernacular name: palo azul). Palo azul has numerous physiological effects that contribute to the prevention of metabolic syndromes, although the mechanism remains unclear. We previously suggested that palo azul has antidiabetic activity via an adipose differentiation effect. Here, we focused on whether palo azul promoted the differentiation of myoblasts. The mouse muscle myoblast cell line C2C12 was cultured and differentiated using horse serum with or without an ethanol extract of palo azul (12.5-200 g/mL). Quantitative real-time polymerase chain reaction was performed to evaluate differentiation markers, including insulin-like growth factor-1 and myogenin. To evaluate myotube formation, myosin heavy-chain (MHC) expression and localization were detected by immunohistochemistry. Palo azul increased the expression of the differentiation markers. Furthermore, immunohistochemistry analysis revealed increased formation of MHC myotubes after palo azul treatment along with increased diameter and fusion indices of the myotubes. The expression level of MHC was also increased. In conclusion, palo azul may increase muscle mass in the body and improve insulin resistance conditions by facilitating the formation of myotubes by promoting myocyte differentiation.


PubMed | JPD Co. and Chiba University
Type: Journal Article | Journal: Pharmacognosy magazine | Year: 2015

Many health experts support the hypothesis that stressful lifestyles are the leading cause of illness, like depression. Therefore, from the standpoint of preventive medicine, it is important to reduce stress. Young green barley leaves are a good natural source of vitamins and minerals, and their juice is widely consumed as a functional food for health reasons in Japan. This study investigated the protective effect of young green barley leaves for stress control.ICR outbred mice were exposed to 3-h sessions of restraint stress. Young green barley leaves (400 and 1,000 mg/kg) were administered orally 1 h before the sessions for 5 days. To analyze voluntary behavior, wheel-running activity was monitored during the dark period. Brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) expression in the whole hippocampus was measured by real-time quantitative polymerase chain reaction.Restraint stress resulted in a significant decrease in voluntary wheel-running behavior, but this decrease was ameliorated by the administration of young green barley leaves. The leaves also enhanced the decreased levels of BDNF mRNA induced by restraint stress; in particular, a significant protective effect was shown in the exon IV variant as compared to vehicle control mice.The findings suggest that young green barley leaves have potent anti-stress properties, as evidenced by preventing decreases in the levels of voluntary wheel-running activity and hippocampal BDNF mRNA in response to restraint stress. Our findings support the possibility that supplementation with young green barley leaves might be beneficial for preventing stress-related psychiatric disorders like depression.


Ueyama H.,JPD Co. | Aotsuka Y.,JPD Co. | Okawa M.,Fukuoka University | Ogura Y.,JPD Co. | And 3 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2011

Lutonarin was isolated from young barley (Hordeum vulgare L. var. nudum Hook) leaves. It has been suggested that lutonarin found in plants, especially in leaves, plays a role in removing reactive oxygen species 6). In this study, we examined the antioxidative effects of lutonarin and related flavones against the free radical DPPH and Fenton's reaction in vitro. The DPPH radical-scavenging activity was higher in the flavones with a catechol struc-ture in the B-ring of the flavone compared to those in which the B-ring contained only a single hydroxyl mole-cule. Furthermore, the higher DPPH radical scavenging activity of saponarin compared to apigenin suggests that the 6-C-and/or 7-O-glucosyl moiety in saponarin stimu-lates its radical scavenging activity. In a comparison of lutonarin with luteolin, the inhibitory action against the oxidation of ethyl linoleate by Fenton's reaction appeared to be weakened by the 6-C-and/or 7-O-glucosyl moiety.


Chen D.H.,Dalian University | Zhang G.Q.,Dalian University | Chen X.,JPD company
Advanced Materials Research | Year: 2013

In order to explore the dynamics performance of WN (Wildhaber-Novikov) gear transmission, according to the WN gear meshing transmission principle and the forced vibration theory with multi-mass and multi-degree of freedom, the nonlinear two degree of freedom forced vibration analysis model of WN gear pairs load meshing was established. Using vibration analysis and experiment, the meshing vibration behavior and tooth root bend strength of WN Gear were synthetically researched. The example calculation and the solution of the motion differential equations were completed by using numerical analysis method. The application foundation is improved for the dynamics design of WN gear transmission. © (2013) Trans Tech Publications, Switzerland.


Saito K.,Yamagata University | Endo R.,Yamagata University | Nozawa Y.,Yamagata University | Minamitani Y.,Yamagata University | Fukata H.,JPD Co.
IEEJ Transactions on Fundamentals and Materials | Year: 2015

It is said that the sterilization by pulsed electric field occurs by pores generated by dielectric breakdown of cell membranes. In the paper, we have discussed what factor by the electric field has generated electric discharge on a cell membrane of bacteria and has been dominant for the sterilization ratio. The dominant factor generating the discharge on the cell membrane has been indicated by organizing the relationship between the condition applying the pulse electric field and the sterilization ratio. As the result, it has been shown that the energy consumption in the membrane of bacteria has worked dominantly in sterilization. It shows the possibility that thermal breakdown takes place by the Joule heat generated by the energy that is consumed in the cell membrane. This result is evidence that electrical breakdown is dominant factor for sterilization in PEF. © 2015 The Institute of Electrical Engineers of Japan.


Trademark
JPD Co. | Date: 2010-04-27

pet food supplements, namely, nutritional additives for cats.


Trademark
JPD Co. | Date: 2012-10-09

Dietary supplements for humans; dietary supplements for animals. Animal foodstuffs; pet food. Vegetable juices (beverage) mainly made from young leaves of barley; vegetable juices (beverage) preparations mainly made from young leaves of barley; vegetable juices (beverage).

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