Jondishapoor University of Medical Science

Ahvāz, Iran

Jondishapoor University of Medical Science

Ahvāz, Iran

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Salamat N.,Khorramshahr Marine Science and Technology University | Erfani Majd N.,Shahid Chamran University | Hashemitabar M.,Jondishapoor University of Medical Science | Mesbah M.,Shahid Chamran University | Ahangarpoor A.,Jondishapoor University of Medical Science
Iranian Journal of Fisheries Sciences | Year: 2010

To study viability and activity of isolated common carp, Cyprinus carpio, ovarian follicular cells (granulosa and theca cells), 17-α-Hydroxy progesterone (17α-OHP) and 17β-Estradiol (E 2) levels were estimated in the culture media of cultivated carp ovarian follicular cells, using radioimmunoassay (RIA). Oocytes were isolated from the ovaries of female carp. Interstitial tissue was manually removed in order to obtain single oocytes surrounded only by the follicular envelope. Such a preparation was trypsinized at room temperature. Follicular cell suspension contained both cells and cell clumps. It was the mixture of theca (T) and granulosa (G) cells. The cells were grown as monolayer in 24-well microplates in M199 medium supplemented with FBS. Culture media were analyzed for estrogen and progesterone content by appropriate radioimmunoassay. Trypsinization of ovarian follicles resulted in the formation of the cell suspension which contained a mixture of G and T cells. The follicular cells attached to glass and grew well during culture period. E2 was the main steroid hormone secreted by cultivated cells. Estrogen secretion increased by 415.52 ± 25 pg/mL at the first 3 days up to 530.25 ± 55.8 pg/ml on day 5 and it didn't change significantly until the end of the experiment. 17α-OHP secretion, however, gradually increased from 23.84 ± 8.2 pg/ml at the beginning of culture up to 35.76 ± 5.4 pg/ml at the end of cultivating. As the result of the present study the fish follicular cells grown in tissue culture were steroidogenically active cells as expressed by the secretion of E 2 and P 4 and The E 2 was a dominant hormone secreted by isolated follicular cells, which it correlated closely with vitellogenes stage.


Salamat N.,Khorramshahr Marine Science and Technology University | Erfani Majd N.,Shahid Chamran University | Hashemitabar H.,Jondishapoor University of Medical science | Mesbah M.,Shahid Chamran University | AhangarPour A.,Jondishapoor University of Medical Science
Iranian Journal of Fisheries Sciences | Year: 2012

Vitellogenic stage carp ovaries were collected aseptically and the cells of ovarian follicles were dispersed by trypsin treatment and cultivated as monolayer. Control cultures were grown in medium M199 alone. Media for experimental cultures were supplemented either with 100 μl ml -1 of CPH and CPS-carp GnRH (cultivated pituitary cell secretion affected by 10 -6, 10 -8, 10 -10 M carp GnRH). The direct effect of these factors on 17-α-Hydroxy progesterone (17α-OHP) and 17β-Estradiol (E2) secretion of isolated follicular cells in cell culture was assayed by appropriate Radioimmunoassay (RIA). According to the results the E2 was a main hormone secreted by cultivated carp granulosa and theca cells. Adding 100 μl ml -1of 10 -6 M CPS-carp GnRH had no significant effect on cultivated granulosa and theca cells endocrine activity (P> 0.05). On the other hand, carp granulosa and theca cells treated with the lower contents of CPS-carp GnRH (10 -8, 10 -10 M) showed a 4.02 and 3.74-fold increase in E2 concentration and 4.27 and 3.64-fold increase in 17α-OHP level, which was significantly higher than CPH (P<0.05). Also, there was a significant increase of steroid secretion in group stimulated with 100 μl ml -1 of CPS and CPH, but there were no significant differences between them (P> 0.05).


Rahimi E.,Islamic Azad University | Momtaz H.,Islamic Azad University | Mohammad Hosseini Anari M.,Islamic Azad University | Alimoradi M.,Jondishapoor University of Medical science | And 2 more authors.
African Journal of Biotechnology | Year: 2012

Human diseases caused by Escherichia coli O157:NM and E. coli O157:H7 strains have been reported throughout the world. In developed countries, serotype O157:H7 represents the major cause of human diseases; however, there have been increasing reports of non-O157 Shiga toxin (Stx)-producing E. coli strains associated with gastrointestinal infections. The present study was conducted to investigate the presence of E. coli O157 and E. coli O157: H7 strains and the presence of virulence genes stx1, stx2, eaeA and ehlyA insolates derived from some traditional dairy products and minced beef meat. A total number of 201 samples including 50 samples from traditional butter, 50 samples from traditional cream, 35 samples from kashk, 30 samples from doogh and 36 samples from minced beef meat were purchased in different supermarkets and retailer shops in Isfahan, Chaharmahal, Bakhtyari and Khuzestan provinces in Iran, over a period of 11-month from August 2010 to May 2011. E. coli non- O157, E. coli O157:NM and E. coli O157:H7 were isolated from 14 samples (7%), 3 samples (1.5%) and 1 sample (out of 50 samples of traditional butter) (0.5%) of the 201 dairy products, respectively, in this study. All the E. coli O157:H7/NM isolates were positive for eaeA and stx1 and/or stx2, and one E. coli O157:H7 isolate was positive for EhlyA. Of the 3 stx positive isolates, 1 and 2 isolates had stx1 and stx2, respectively. To our knowledge, the present study is the first report on isolation and identification of E. coli O157 from traditional butter and cream samples in Iran. © 2012 Academic Journals.


Varedi P.,Shariati Hospital | Varedi P.,Jondishapoor University of Medical science | Shirani G.,Shariati Hospital | Bohluli B.,University of Tehran | And 2 more authors.
Journal of Oral and Maxillofacial Surgery | Year: 2013

Lateral osteotomy is usually performed to narrow a wide nasal base, widen a narrow nasal base, straighten deviated nasal bones, or close an (iatrogenic) open roof deformity. Based on the approach used, nasal osteotomy can be classified as external or internal. Although nasal osteotomy is an essential part of cosmetic rhinoplasty, it can be very difficult for young surgeons to perform external osteotomy. This article focuses on external osteotomy and its essential concepts. In addition, a new splint-guide method is introduced, which can help young surgeons perform external osteotomy in a very safe and predictable manner. © 2013 American Association of Oral and Maxillofacial Surgeons.


Oveisi M.R.,Tehran University of Medical Sciences | Sadeghi N.,Tehran University of Medical Sciences | Jannat B.,Food and Drug Laboratory Research Center | Hajimahmoodi M.,Tehran University of Medical Sciences | And 2 more authors.
Iranian Journal of Basic Medical Sciences | Year: 2011

Objective(s): Bone is a dynamic tissue that is continuously renewed throughout life by the process of bone remodeling. Antioxidant system might be involved in the pathogenesis of bone loss, so the aim of this study was to evaluate the total antioxidant capacity (TAC), vitamin C and vitamin E levels of plasma besides measuring enzymatic antioxidants, superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) enzymes activity in Iranian osteoporotic women comparing to the control group. Materials and Methods: Bone mineral density (BMD) of the femoral neck and lumbar spine was measured by dual x-ray absorptiometry. The participants were divided into groups: a) total participants (-3.9 ≤ T-score ≤ 3.6) including 192 women, b) the control group (T-score ≥ -1) including 76 women, c) the total patients (T-score < -1) including 76 women. Then, plasma TAC, vitamin C levels, SOD and GR activities, erythrocyte CAT were measured using spectrophotometrical methods separately, and for vitamin E by HPLC analysis. Results: Comparing the control group and osteoporotic women showed that: a) plasma levels for vitamin C and erythrocyte CAT were markedly lower in the patients than in the controls, but plasma activity of TAC, SOD and GR were significantly higher, respectively. b) the differences were higher between control and patients with severe disease (T-score <-1.7) comparing to patients in the group with milder disease (-1.7 ≤ T-score <-1). c) Femoral neck BMD adjusted with age and BMI showed a positive and significant correlation with plasma levels of vitamin C in all infjects, but this relation was reverse or negative for TAC, SOD and GR. Conclusion: It seems that a physiologic increase in the amount of some antioxidants occurs in osteoporosis; even though this amount may not be sufficient for the human body requirements.


Dalvand P.,Jondishapoor University of Medical science | Peymani P.,Shiraz University of Medical Sciences | Maharlooei M.K.,Shiraz University of Medical Sciences | Ziaee M.,Shiraz University of Medical Sciences | Afifi S.,Shiraz University of Medical Sciences
Current Topics in Pharmacology | Year: 2010

Diabetes is a disease recognized by elevation in some blood factors like glucose, amino acids, free fatty acids etc, some impairment in insulin secretion or resistance to insulin etc. There are mainly two types of diabetes: type 1, in which there is an abnormal absence of insulin secretion, and type 2, which primarily is due to resistance to insulin while its receptors are normal or more than its amount. After some time pancreas won't be able to secrete enough insulin which causes post prandial hyper glycemia and after a while hyper glycemia would be an ever present sign, not just after meals. Insulin injection is necessary in this step. Many diabetics require up to four insulin injections per day, which can be quite inconvenient. The formulation of dietary insulin capable of eluding proteolytic digestion has proven to be challenging. In addition, the drug must pass through the stomach; thus, a synthetic pH-sensitive tablet would be helpful. More specifically, the insulin-containing tablet should be stable in the acidic environment of the stomach, but it should release its contents in the basic environment of the duodenum. We propose to design a bilayer tablet composed of an outer layer containing Antiproteases to neutralize the effect of pancreatic proteases and an inner layer of insulin. As the outer layer is revealed after encountering a basic environment, the Antiproteases start to be released and neutralize the surrounding digestive proteases. This neutralization allows sustained release of insulin from the inner layer of the tablet. The measured release of the drug allows for better absorption in more distal parts of the small intestine.


Foroughmand A.M.,Shahid Chamran University | Nikkhah E.,Shahid Chamran University | Galehdari H.,Shahid Chamran University | Jadbabaee M.H.,Jondishapoor University of Medical science
Cell Journal | Year: 2015

Objective: Coronary artery disease (CAD) is a multi-factorial and heterogenic disease with atherosclerosis plaques formation in internal wall of coronary artery. Plaque formation results to limitation of the blood reaching to myocardium leading to appearance of some problems, such as ischemia, sudden thrombosis veins and myocardial infarction (MI). Several environmental and genetic factors are involved in prevalence and incident of CAD as follows: hypertension, high low density lipoprotein-cholesterol (LDL-C), age, diabetes mellitus, family history of early-onset heart disease and smoking. According to genome wide association studies (GWAS), five polymorphisms in the 9p21 locus seem to be associated with the CAD. We aimed to evaluate the remarkable association of two polymorphisms at 9p21 locus, rs1333049 and rs10757274, with CAD. Materials and Methods: This experimental study was conducted in Golestan, Aria Hospitals and Genetics Lab of Shahid Chamran University in the city of Ahvaz, Iran, in 2010- 2011. The collected blood samples belonging to 170 CAD patients (case group) and 100 healthy individuals (control group) were analyzed by tetra-primer amplification refractory mutation system (ARMS)-polymerase chain reaction (PCR) technique. The results were analyzed using software package used for statistical analysis (SPSS; SPSS Inc., USA) version 16. A value of p<0.05 and an odd ratio (OR) with 95% confidence intervals (CI) were considered significant. Results: The frequencies of CC, CG and GG genotypes for rs1333049 polymorphism in patients were 18.2, 65.3 and 16.5%, while in controls, the related values were 25, 67 and 8%, respectively. GG genotypes of rs1333049 polymorphism in CAD patients were more than control cases (OR: 0.354, 95%CI: 0.138-0.912, p=0.032). The frequencies of AA, AG and GG genotypes for rs10757274 in CAD patients were 8.2, 58.3 and 33.5%, while in controls, the related values were 35, 63 and 2%, respectively. GG Genotype in rs10757274 polymorphism in CAD patients was found more than control cases (OR: 0.014, 95% CI: 0.003 -0.065, p=0.0001). Conclusion: The rs1333049 polymorphism at 9p21 locus shows a weak association with CAD, whereas rs10757274 polymorphism reveals a significant association with CAD. These variants may help the identification of patients with increased risk for coronary artery disease.


PubMed | Jondishapoor University of Medical science
Type: Journal Article | Journal: Research in pharmaceutical sciences | Year: 2011

Chlorogenic acid, a pharmacologically important compound, is a phenolic compound that occurs in certain commonly used medicinal herbs. We looked for the presence of this compound in the callus cultures of Varthemia persica DC (var. persica). We have evaluated the conditions for establishment of callus cultures of V. persica and the in vitro production of chlorogenic acid. Callus was initiated by culturing seedling of V. persica on MS basal medium supplemented with different concentrations of kinetin, naphthalene acetic acid and 2,4-diphenoxy acetic acid. Also, the influence of light, and phytohormones on the production of chlorogenic acid was examined. Kinetin stimulated the production of chlorogenic acid. Replacement of 2,4-diphenoxy acetic acid with naphthalene acetic acid did not alter the chlorogenic acid production. The ability to induce the accumulation of chlorogenic acid in the V. persica callus cultures offers an opportunity to produce a phenolic compound with therapeutic value.


PubMed | Jondishapoor University of Medical science and Shahid Chamran University
Type: Journal Article | Journal: Cell journal | Year: 2015

Coronary artery disease (CAD) is a multi-factorial and heterogenic disease with atherosclerosis plaques formation in internal wall of coronary artery. Plaque formation results to limitation of the blood reaching to myocardium leading to appearance of some problems, such as ischemia, sudden thrombosis veins and myocardial infarction (MI). Several environmental and genetic factors are involved in prevalence and incident of CAD as follows: hypertension, high low density lipoprotein-cholesterol (LDL-C), age, diabetes mellitus, family history of early-onset heart disease and smoking. According to genome wide association studies (GWAS), five polymorphisms in the 9p21 locus seem to be associated with the CAD. We aimed to evaluate the remarkable association of two polymorphisms at 9p21 locus, rs1333049 and rs10757274, with CAD.This experimental study was conducted in Golestan, Aria Hospitals and Genetics Lab of Shahid Chamran University in the city of Ahvaz, Iran, in 2010- 2011. The collected blood samples belonging to 170 CAD patients (case group) and 100 healthy individuals (control group) were analyzed by tetra-primer amplification refractory mutation system (ARMS)-polymerase chain reaction (PCR) technique. The results were analyzed using software package used for statistical analysis (SPSS; SPSS Inc., USA) version 16. A value of p<0.05 and an odd ratio (OR) with 95% confidence intervals (CI) were considered significant.The frequencies of CC, CG and GG genotypes for rs1333049 polymorphism in patients were 18.2, 65.3 and 16.5%, while in controls, the related values were 25, 67 and 8%, respectively. GG genotypes of rs1333049 polymorphism in CAD patients were more than control cases (OR: 0.354, 95%CI: 0.138-0.912, p=0.032). The frequencies of AA, AG and GG genotypes for rs10757274 in CAD patients were 8.2, 58.3 and 33.5%, while in controls, the related values were 35, 63 and 2%, respectively. GG Genotype in rs10757274 polymorphism in CAD patients was found more than control cases (OR: 0.014, 95% CI: 0.003 -0.065, p=0.0001).The rs1333049 polymorphism at 9p21 locus shows a weak association with CAD, whereas rs10757274 polymorphism reveals a significant association with CAD. These variants may help the identification of patients with increased risk for coronary artery disease.

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