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Li Y.,CAS Kunming Institute of Zoology | Li Y.,Joint Laboratory of Bioresources and Molecular Research in Common Diseases | Li Y.,University of Chinese Academy of Sciences | Huang W.,Third Peoples Hospital | And 8 more authors.
Mitochondrial DNA | Year: 2016

Mitochondrial DNA (mtDNA) is crucial to mitochondria in energy production and other physiological functions. When lowlanders arrive at high altitude, the mitochondrial content tends to decrease. However, the mtDNA content of native highlanders share the same feature as lowlanders remains unknown. It is also interesting to dissect the other changes in blood plasma that might accompany the change of mtDNA content. To address these issues, we recruited 241 Tibetan subjects in Tibet and 220 Han subjects in Shaanxi province. Relative mtDNA copy number and blood biochemical indexes were measured. Results show that relative mtDNA copy number in Tibetans is significantly lower as compared to Han subjects; sex, age, blood glucose, triglyceride and total cholesterol show no influence on mtDNA content, but carbon dioxide combining power is negatively correlated with mtDNA content. These results indicate that an increase in CO2 combining power along with lower mtDNA content may provide adaptive potential. © 2014 Informa UK Ltd.

Peng M.-S.,CAS Kunming Institute of Zoology | Peng M.-S.,Joint Laboratory of Bioresources and Molecular Research in Common Diseases | Peng M.-S.,University of Chinese Academy of Sciences | He J.-D.,CAS Kunming Institute of Zoology | And 7 more authors.
BMC Evolutionary Biology | Year: 2011

Abstract. Background: Hainan Island is located around the conjunction of East Asia and Southeast Asia, and during the Last Glacial Maximum (LGM) was connected with the mainland. This provided an opportunity for the colonization of Hainan Island by modern human in the Upper Pleistocene. Whether the ancient dispersal left any footprints in the contemporary gene pool of Hainan islanders is debatable. Results: We collected samples from 285 Li individuals and analyzed mitochondrial DNA (mtDNA) variations of hypervariable sequence I and II (HVS-I and II), as well as partial coding regions. By incorporating previously reported data, the phylogeny of Hainan islanders was reconstructed. We found that Hainan islanders showed a close relationship with the populations in mainland southern China, especially from Guangxi. Haplotype sharing analyses suggested that the recent gene flow from the mainland might play important roles in shaping the maternal pool of Hainan islanders. More importantly, haplogroups M12, M7e, and M7c1* might represent the genetic relics of the ancient population that populated this region; thus, 14 representative complete mtDNA genomes were further sequenced. Conclusions: The detailed phylogeographic analyses of haplogroups M12, M7e, and M7c1* indicated that the early peopling of Hainan Island by modern human could be traced back to the early Holocene and/or even the late Upper Pleistocene, around 7 - 27 kya. These results correspond to both Y-chromosome and archaeological studies. © 2011 Peng et al; licensee BioMed Central Ltd.

He Y.-H.,CAS Kunming Institute of Zoology | He Y.-H.,Joint Laboratory of Bioresources and Molecular Research in Common Diseases | Lu X.,CAS Kunming Institute of Zoology | Lu X.,Joint Laboratory of Bioresources and Molecular Research in Common Diseases | And 5 more authors.
Aging | Year: 2014

Human lifespan is determined greatly by genetic factors and some investigations have identified putative genes implicated in human longevity. Although some genetic loci have been associated with longevity, most of them are difficult to replicate due to ethnic differences. In this study, we analyzed the association of 18 reported gene single nucleotide polymorphisms (SNPs) with longevity in 1075 samples consisting of 567 nonagenarians/centenarians and 508 younger controls using the GenomeLab SNPstream Genotyping System. Our results confirm the association of the forkhead box O3 (FOXO3) variant (rs13217795) and the ATM serine/threonine kinase (ATM) variant (rs189037) genotypes with longevity (p=0.0075 and p=0.026, using the codominant model and recessive model, respectively). Of note is that we first revealed the association of insulin-like growth factor binding protein 3 (IGFBP-3) gene polymorphism rs11977526 with longevity in Chinese nonagenarians/centenarians (p=0.033 using the dominant model and p=0.035 using the overdominant model). The FOXO3 and IGFBP-3 form important parts of the insulin/insulin-like growth factor-1 signaling pathway (IGF-1) implicated in human longevity, and the ATM gene is involved in sensing DNA damage and reducing oxidative stress, therefore our results highlight the important roles of insulin pathway and oxidative stress in the longevity in the Chinese population. © He et al.

Li R.,CAS Kunming Institute of Zoology | Li R.,Joint Laboratory of Bioresources and Molecular Research in Common Diseases | Li R.,Yunnan University | Wang H.,CAS Kunming Institute of Zoology | And 8 more authors.
Mitochondrial DNA | Year: 2015

Macaca fascicularis, known as the long-tailed macaque, is widely distributed in southern of East Asia and Southeast Asia. It was one of the most commonly used non-human primates in biomedical research. Thus, to illustrate the maternal phylogenetic status of M. fascicularis in primates based on the whole mitochondrial DNA (mtDNA) genome and determine a reference sequence for future population genetic studies by taking mtDNA as molecular marker, in this study, the high quality whole mtDNA genome of M. fascicularis was amplified and sequenced. Our data showed that the whole mtDNA genome of M. fascicularis includes 16,571 base pairs (bps). Further phylogenetic analyses of M. fascicularis were performed by incorporating the 83 available whole mtDNA genomes belonging to 77 primate species with Tupaia belangeri as out-group. Our result supported that M. fascicularis belongs to Macaca. Cercopithecinae. Cercopithecidae. Anthropoidea. Primates, which has the closest genetic affinity with Macaca mulatta. In addition, the ancestral divergence between the tarsier and other primate species was supported with evidence from the whole mtDNA genomes. © 2013 Informa UK Ltd.

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