Joint Genomic Center

Sofia, Bulgaria

Joint Genomic Center

Sofia, Bulgaria
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Banov M.,Institute of Soil Science | Atanassov A.,Joint Genomic Center | Fartzov K.,Joint Genomic Center | Ivanov A.,Agricultural University of Plovdiv
Bulgarian Journal of Agricultural Science | Year: 2016

The most important factors for growing grape and for wine production are soil and climate. The presented material considered soil and climatic characteristics of the territory of a particular vineyard. Growing grape variety is "Mavrud". Subject to the soil and climate characteristics is agricultural land - plot No 58058, cadastral ? 01001, 01002 01003, 01004, village Pravishte, municipality Saedinenie, located in the Loziata neighborhood, vegetated with vines "Mavrud". The data for climate characteristics show, that the area is suitable for growing grape and for wine production. The soil research at the investigated region was made on the basis of archive data and field and on site research. Samples were taken on site. Those samples were then analyzed regarding the following parameters: distribution of the mechanical fractions; contents of organic matter (humus); medium reaction (pH in water solution); general phosphorus; general potassium; carbonates (CaCO3). A soil survey was carried out in order to determine the soil cover in the Pravishte village. The results of the soil research show that the subject area falls into one soil type - Leached Vertisols. A morphological description has been made, physico-chemical properties are determined and a summary of the above mentioned soil type is prepared. © 2016, National Centre for Agrarian Sciences. All rights reserved.


Danova K.,Bulgarian Academy of Science | Nikolova-Damianova B.,Bulgarian Academy of Science | Denev R.,Joint Genomic Center | Dimitrov D.,Bulgarian Academy of Science
Plant Cell, Tissue and Organ Culture | Year: 2012

Hypericin is a widely studied phytopharmaceutical produced by the more developed representatives of the Hypericum genus. We established that Gamborg's (G5) vitamins significantly raised hypericins levels in H. rumeliacum and H. tetrapterum shoot cultures in comparison with the Murashige and Skoog's (MS) vitamin supplementation. To elucidate this effect, we further examined the impact of the two vitamin formulations on the polyphenolic compounds, morphological development, hydrogen peroxide (H 2O 2) and malondialdehyde (MDA) levels in the two species, as well as in hypericin non-producing H. calycinum. It was found that in all three species Gamborg vitamins decreased the polyphenolic compounds content, stimulated multiplication, decreased leaf compactness and increased dark glands density per square millimeter (for the two hypericin producing species) as compared to MS vitamins. In addition, the raised levels of hypericins in H. rumeliacum and H. tetrapterum, were also connected to increased content of H 2O 2 and MDA in vitro. Moreover, the values of these two parameters were considerably lower in hypericin non-producing H. calycinum, implying that oxidative stress either by increased metabolic activity, connected with higher hypericin production, or by a possible auto-toxic effect of the high amounts of pro-oxidant hypericin itself could be induced in Hypericum in vitro. © 2012 Springer Science+Business Media B.V.


Simova S.,Bulgarian Academy of Science | Atanassov A.,Joint Genomic Center | Shishiniova M.,Joint Genomic Center | Bankova V.,Bulgarian Academy of Science
Food Chemistry | Year: 2012

An NMR-based approach for rapid differentiation of oak honeydew honey from all other honey types (floral and other honeydew honeys) was proposed. It is based on the identification of the signals of the protons and the carbon of the methylene group of quercitol in the 1H and 13C NMR spectra of honey. The presence of quercitol was supported by TOCSY spectroscopy. Quercitol is a deoxyinositol which is regarded as a good taxonomic marker for the genus Quercus. All samples of oak honeydew honey contained quercitol, while in floral honey samples and honeydew honeys from fir and spruce it was absent. In addition, the described approach is promising with respect to quercitol quantification in honey by qNMR. © 2012 Elsevier Ltd. All rights reserved.


Danova K.,Bulgarian Academy of Science | Nikolova-Damianova B.,Bulgarian Academy of Science | Denev R.,Joint Genomic Center | Markovska Y.,Sofia University
Plant Growth Regulation | Year: 2012

Due to its high hypericin and pseudohypericin in vitro biosynthetic capacity, the Balkan endemic Hypericum rumeliacum was selected as a prospective candidate for long-term preservation of valuable medicinal plant germplasm. Initial cryopreservation experiments were previously conducted based on the successful protocol established and reported for the widely studied H. perforatum. This is the first report on the impact of pre-culture duration on the short- and long-term in vitro recovery of the biosynthetic potential and antioxidant defense system of H. rumeliacum cryopreserved by vitrification. Cryopreservation did not impair the phenolics and flavonoids production of the regenerated plants. Moreover, hypericin and pseudohypericin levels even increased substantially in one of the regenerated lines, reaching yields from 0. 107 and 0. 752 mg g-1 DW in the control up to 0. 277 and 1. 112 mg g-1 DW for hypericin and pseudohypericin, respectively. However, the physical injury stress of the pre-culture treatment manipulations affected the physiological status of regenerants in a time dependent manner. Within 6 months after thawing, regenerants with the highest oxidative stress after pre-culture, were characterized with an augmentation of antioxidant metabolites such as phenolics, flavonoids, glutathione and ascorbic acid as well as increased antioxidant enzymatic activities in comparison with both the non-frozen control and the regenerants with the lowest pre-culture oxidative stress. Then, after 18 months of recovery, the same first H. rumeliacum group displayed a marked drop of enzymatic antioxidant activity as compared with the other groups of plants. Further research is needed to target oxidative stress alleviation to optimize H. rumeliacum cryopreservation protocol. © 2012 Springer Science+Business Media B.V.


Abumhadi N.,Agro Bio Institute | Todorovska E.,Agro Bio Institute | Assenov B.,Agro Bio Institute | Tsonev S.,Agro Bio Institute | And 5 more authors.
Bulgarian Journal of Agricultural Science | Year: 2012

During the recent decades, global climate change recognized as one of the most serious challenges facing the world - its people, the environment and its economies. Agriculture will face significant challenges in the 21st century, largely due to the need to increase global food supply under the declining availability of soil and water resources and increasing threats from climate change. Nonetheless, these challenges also offer opportunities to develop and promote food and livelihood systems that have greater environmental, economic and social resilience to risk. It is clear that success in meeting these challenges will require both the application of current multidisciplinary knowledge, and the development of a range of technical and institutional innovations. The changing climate is also a major challenge for agriculture and agricultural policy-making. Agriculture needs to address the double challenge of reducing its greenhouse gases emissions (GHGs) while at the same time adapting to projected impacts of climate change. In 2003, Nobel Laureate Richard E. Smalley outlined Humanity's Top Ten Problems for the next 50 years, in a talk given for the MIT Enterprise Forum. According to Professor Smalley, the biggest problems facing humanity are: Energy, Water, Food, Environment, Poverty, Terrorism and War, Disease, Education, Democracy and Population. The goal of the present study is to summarize main findings regarding some of these problems focusing on changes in climate conditions and to provide an overview of major challenges facing the global food and agricultural system in the 21st century and the impacts of the climate change. In the future, the review studies will focus on expanding the research for some specific challenges of climate change (Drought, cold, salinity) and its impact on agriculture.


Bankova V.,Bulgarian Academy of Science | Atanassov A.,Joint Genomic Center | Denev R.,Joint Genomic Center | Shishinjova M.,Joint Genomic Center
Biotechnology and Biotechnological Equipment | Year: 2012

This mini-review summarizes the most important results concerning the health-promoting potential of Bulgarian honeys, royal jelly and propolis. Bulgarian honey, propolis and royal jelly have demonstrated remarkable biological activities, such as antibacterial, antiviral, antioxidant, immunostimulating, etc., and deserve further detailed studies. The most promising perspectives for future research and utilization of these valuable Bulgarian bee products are outlined.


Tzenkova R.,Joint Genomic Center | Kamenarska Z.,Joint Genomic Center | Draganov A.,Rosbio Bulgaria Ltd | Atanassov A.,Joint Genomic Center
Biotechnology and Biotechnological Equipment | Year: 2010

The Artemisia annua essential oil was obtained by hydro distillation of species growing wild in Bulgaria and was analyzed by GC/MS. Thirty six compounds were identified. The main ones were a-caryophillene (24.73%), α-cuvebene (13.53%), α-copaene (7.42%), α-selinene (8.21%) artemisia ketone (8.45%) and camphor (3.61%). The composition of the essential oil appeared to be different from those of species growing in other geographic locations.


Todorovska E.,Agro Bio Institute | Atanassov A.,Joint Genomic Center | Vassilev D.,Agro Bio Institute
Genetika | Year: 2010

The classical concepts in plant and livestock selection for economically important quantitative traits traditionally are based on phenotypic records, aiming at improvement of the traits by obtaining better genetic gain. The increase in genetic variation together with shortening of the generation interval is the major target of long term improvement of methods and tools for selection activities. The discoveries and implementations of biotechnology and molecular biology for selection purposes provide a stable background for generating of new knowledge and practical use in agricultural research and practice as well as to meet the growing demand for more and with better quality food and feed. The innovations in molecular knowledge related to practical selection aside with the quick quantification in breeding schemes allowed to reconsider the opportunities for sustainable development of selection methods for improvement of the traits of interest in agriculture, the quick invention and practical application of new high-throughput technologies for studying of the genomic variation, evolution, translation of proteins and metabolite determination altogether put in an open and communicative environment of information technologies provide a new holistic platform for better research and more knowledge for practical application of selection decisions.


PubMed | Joint Genomic Center and Bulgarian Academy of Science
Type: Journal Article | Journal: Food chemistry | Year: 2014

An NMR-based approach for rapid differentiation of oak honeydew honey from all other honey types (floral and other honeydew honeys) was proposed. It is based on the identification of the signals of the protons and the carbon of the methylene group of quercitol in the (1)H and (13)C NMR spectra of honey. The presence of quercitol was supported by TOCSY spectroscopy. Quercitol is a deoxyinositol which is regarded as a good taxonomic marker for the genus Quercus. All samples of oak honeydew honey contained quercitol, while in floral honey samples and honeydew honeys from fir and spruce it was absent. In addition, the described approach is promising with respect to quercitol quantification in honey by qNMR.

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