Joint Center for Biosciences

Incheon, South Korea

Joint Center for Biosciences

Incheon, South Korea
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The present invention provides an antiseptic and antibiotic composition containing a Pseudomonas aeruginosa culture solution extract as an active ingredient. The present invention provides a composition for preventing or treating acne, containing a Pseudomonas aeruginosa culture solution extract as an active ingredient. The composition containing a Pseudomonas aeruginosa culture solution extract, of the present invention, exhibits a broad antibiotic spectrum and a high antioxidant effect against various bacteria, and the composition can be applied to a cosmetic composition such as a cosmetic product and can also be useful for antibiotic, sterilizing and antiseptic purposes in various fields such as food, medical supplies, agricultural chemicals and household items. The present invention identifies a specific active ingredient causing the antibiotic effect of a Pseudomonas aeruginosa culture solution extract, and provides the structure thereof.


The present invention provides an antiseptic and antibiotic composition containing a Pseudomonas aeruginosa culture solution extract as an active ingredient. The present invention provides a composition for preventing or treating acne, containing a Pseudomonas aeruginosa culture solution extract as an active ingredient. The composition containing a Pseudomonas aeruginosa culture solution extract, of the present invention, exhibits a broad antibiotic spectrum and a high antioxidant effect against various bacteria, and the composition can be applied to a cosmetic composition such as a cosmetic product and can also be useful for antibiotic, sterilizing and antiseptic purposes in various fields such as food, medical supplies, agricultural chemicals and household items. The present invention identifies a specific active ingredient causing the antibiotic effect of a Pseudomonas aeruginosa culture solution extract, and provides the structure thereof.


Kwiatkowski W.,Salk Institute for Biological Studies | Kwiatkowski W.,Joint Center for Biosciences | Gray P.C.,Salk Institute for Biological Studies | Choe S.,Salk Institute for Biological Studies | Choe S.,Joint Center for Biosciences
Trends in Pharmacological Sciences | Year: 2014

TGF-β superfamily ligands govern normal tissue development and homeostasis, and their dysfunction is a hallmark of many diseases. These ligands are also well defined both structurally and functionally. This review focuses on TGF-β superfamily ligand engineering for therapeutic purposes, in particular for regenerative medicine and musculoskeletal disorders. We describe the key discovery that structure-guided mutation of receptor-binding epitopes, especially swapping of these epitopes between ligands, results in new ligands with unique functional properties that can be harnessed clinically. Given the promising results with prototypical engineered TGF-β superfamily ligands, and the vast number of such molecules that remain to be produced and tested, this strategy is likely to hold great promise for the development of new biologics. © 2014 Elsevier Ltd. All rights reserved.


Yang Z.,University of Rochester | Yang Z.,Joint Center for Biosciences | Hayes J.J.,University of Rochester
Biochemistry | Year: 2011

We previously reported that reconstituted nucleosomes undergo sequence-dependent translational repositioning upon removal of the core histone tail domains under physiological conditions, indicating that the tails influence the choice of position. We report here that removal of the core histone tail domains increases the exposure of the DNA backbone in nucleosomes to hydroxyl radicals, a nonbiased chemical cleavage reagent, indicative of an increase in the motility of the DNA on the histone surface. Moreover, we demonstrate that the divalent cations Mg 2+ and Ca 2+ can replace the role of the tail domains with regard to stabilization of histone-DNA interactions within the nucleosome core and restrict repositioning of nucleosomes upon tail removal. However, when nucleosomes were incubated with Mg 2+ after tail removal, the original distribution of translational positions was not re-established, indicating that divalent cations increase the energy barrier between translational positions rather than altering the free energy differences between positions. Interestingly, other divalent cations such as Zn 2+, Fe 2+, Co 2+, and Mn 2+ had little or no effect on the stability of histone-DNA interactions within tailless nucleosomes. These results support the idea that specific binding sites for Mg 2+ and Ca 2+ ions exist within the nucleosome and play a critical role in nucleosome stability that is partially redundant with the core histone tail domains. © 2011 American Chemical Society.


Shon S.-M.,Dongguk University | Choi Y.,National Cancer Center | Kim J.-Y.,Dongguk University | Lee D.K.,Joint Center for Biosciences | And 3 more authors.
Arteriosclerosis, Thrombosis, and Vascular Biology | Year: 2013

Objective-To investigate whether an intravenously injected cathepsin-B activatable theranostic agent (L-SR15) would be cleaved in and release a fluorescent agent (chlorin-e6) in mouse atheromata, allowing both the diagnostic visualization and therapeutic application of these fluorophores as photosensitizers during photodynamic therapy to attenuate plaquedestabilizing cathepsin-B activity by selectively eliminating macrophages. Approach and Results-Thirty-week-old apolipoprotein E knock-out mice (n=15) received intravenous injection of L-SR15 theranostic agent, control agent D-SR16, or saline 3× (D0, D7, D14). Twenty-four hours after each injection, the bilateral carotid arteries were exposed, and Cy5.5 near-infrared fluorescent imaging was performed. Fluorescent signal progressively accumulated in the atheromata of the L-SR15 group animals only, indicating that photosensitizers had been released from the theranostic agent and were accumulating in the plaque. After each imaging session, photodynamic therapy was applied with a continuous-wave diode-laser. Additional near-infrared fluorescent imaging at a longer wavelength (Cy7) with a cathepsin-B-sensing activatable molecular imaging agent showed attenuation of cathepsin- B-related signal in the L-SR15 group. Histological studies demonstrated that L-SR15-based photodynamic therapy decreased macrophage infiltration by inducing apoptosis without significantly affecting plaque size or smooth muscle cell numbers. Toxicity studies (n=24) showed that marked erythematous skin lesion was generated in C57/BL6 mice at 24 hours after intravenous injection of free chlorin-e6 and ultraviolet light irradiation; however, L-SR15 or saline did not cause cutaneous phototoxicity beyond that expected of ultraviolet irradiation alone, neither did we observe systemic toxicity or neurobehavioral changes. Conclusions-This is the first study showing that macrophage-secreted cathepsin-B activity in atheromata could be attenuated by photodynamic therapy using a protease-mediated theranostic agent. © 2013 American Heart Association, Inc.


Kuo M.M.C.,Joint Center for Biosciences | Kim S.,Joint Center for Biosciences | Tseng C.-Y.,Joint Center for Biosciences | Jeon Y.-H.,Joint Center for Biosciences | And 2 more authors.
Biomaterials | Year: 2014

BMP-9, whose expression is highest in liver cells, has been demonstrated to regulate expression of enzymes involved in glucose homeostasis. However, the underlying mechanism of this effect has yet to be elucidated. We observed that MB109, a recombinant BMP-9 derivative, enhanced brown adipogenesis of human adipose tissue derived stem cells. With this observation of the cell culture system, we hypothesized that MB109 may be able to improve glucose metabolism by regulating expression of brown adipogenic genes. Systemic intraperitoneal injection of MB109 (200 μg/kg/wk) suppressed weight gaining of high fat diet-induced obese mice by reducing sizes of white adipocytes and decreased 16 h fasting blood glucose levels without changing food consumption or apparent behavioral performances. MB109 induced expression of brown adipogenic genes in the subcutaneous but not in the visceral fat tissues from the mice fed with high fat diet. In addition, systematic injection of MB109 enhanced fatty acid synthase expression in the liver of obese mice, which may help attenuate an obesity-associated increase of blood glucose levels. Our results demonstrate a role of BMP-9 in brown adipogenesis and suppressing pathophysiology of high fat diet-induced obesity, presumably through the activin receptor like kinase 1 signaling pathway. © 2013 Elsevier Ltd.


Patent
Joint Center For Biosciences | Date: 2014-10-16

The disclosure provides for the expression and purification of bioactive recombinant bone morphogenetic protein (BMP)-9 from bacteria (MB109) and the use of the MB109 to treat various diseases and disorders, including cancer and obesity associated disorders.


Patent
Joint Center For Biosciences and Salk Institute for Biological Studies | Date: 2012-09-05

Provided herein are methods and compositions for efficient accumulation of structural information (e.g., three dimensional structural information) for amino acid sequences.


Patent
Salk Institute for Biological Studies and Joint Center For Biosciences | Date: 2014-12-30

The present disclosure relates to chimeric polypeptide having TGF-beta activity, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.


Patent
Salk Institute for Biological Studies and Joint Center For Biosciences | Date: 2010-02-24

The present disclosure relates to chimeric polypeptide having TGF-beta activity, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.

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