Villa General Mitre, Argentina

John F. Kennedy Argentine University

www.kennedy.edu.ar
Villa General Mitre, Argentina

The Universidad Argentina John F. Kennedy was founded by Miguel Herrera Figueroa, JD, on April 4, 1964 in Buenos Aires, Argentina. The university has 18 units in the City of Buenos Aires and two in Buenos Aires Province. In 2008, it had 39 academic departments and a students department, where activities related to sports are developed with the Kennedy Choir and the Theater Workshop. Wikipedia.

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Guarracino J.F.,University of Buenos Aires | Cinalli A.R.,University of Buenos Aires | Fernandez V.,John F. Kennedy Argentine University | Roquel L.I.,John F. Kennedy Argentine University | Losavio A.S.,University of Buenos Aires
Neuroscience | Year: 2016

It is known that adenosine 5'-triphosphate (ATP) is released along with the neurotransmitter acetylcholine (ACh) from motor nerve terminals. At mammalian neuromuscular junctions (NMJs), we have previously demonstrated that ATP is able to decrease ACh secretion by activation of P2Y receptors coupled to pertussis toxin-sensitive Gi/o protein. In this group, the receptor subtypes activated by adenine nucleotides are P2Y12 and P2Y13. Here, we investigated, by means of pharmacological and immunohistochemical assays, the P2Y receptor subtype that mediates the modulation of spontaneous and evoked ACh release in mouse phrenic nerve-diaphragm preparations. First, we confirmed that the preferential agonist for P2Y12-13 receptors, 2-methylthioadenosine 5'-diphosphate trisodium salt hydrate (2-MeSADP), reduced MEPP frequency without affecting MEPP amplitude as well as the amplitude and quantal content of end-plate potentials (EPPs). The effect on spontaneous secretion disappeared after the application of the selective P2Y12-13 antagonists AR-C69931MX or 2-methylthioadenosine 5'-monophosphate triethylammonium salt hydrate (2-MeSAMP). 2-MeSADP was more potent than ADP and ATP in reducing MEPP frequency. Then we demonstrated that the selective P2Y13 antagonist MRS-2211 completely prevented the inhibitory effect of 2-MeSADP on MEPP frequency and EPP amplitude, whereas the P2Y12 antagonist MRS-2395 failed to do this. The preferential agonist for P2Y13 receptors inosine 5'-diphosphate sodium salt (IDP) reduced spontaneous and evoked ACh secretion and MRS-2211 abolished IDP-mediated modulation. Immunohistochemical studies confirmed the presence of P2Y13 but not P2Y12 receptors at the end-plate region. Disappearance of P2Y13 receptors after denervation suggests the presynaptic localization of the receptors. We conclude that, at motor nerve terminals, the Gi/o protein-coupled P2Y receptors implicated in presynaptic inhibition of spontaneous and evoked ACh release are of the subtype P2Y13. This study provides new insights into the types of purinergic receptors that contribute to the fine-tuning of cholinergic transmission at mammalian neuromuscular junction. © 2016 IBRO.


Cinalli A.R.,CONICET | Guarracino J.F.,CONICET | Fernandez V.,John F. Kennedy Argentine University | Roquel L.I.,John F. Kennedy Argentine University | Losavio A.S.,CONICET
British Journal of Pharmacology | Year: 2013

Background and Purpose The role of inosine at the mammalian neuromuscular junction (NMJ) has not been clearly defined. Moreover, inosine was classically considered to be the inactive metabolite of adenosine. Hence, we investigated the effect of inosine on spontaneous and evoked ACh release, the mechanism underlying its modulatory action and the receptor type and signal transduction pathway involved. Experimental Approach End-plate potentials (EPPs) and miniature end-plate potentials (MEPPs) were recorded from the mouse phrenic-nerve diaphragm preparations using conventional intracellular electrophysiological techniques. Key Results Inosine (100 μM) reduced MEPP frequency and the amplitude and quantal content of EPPs; effects inhibited by the selective A3 receptor antagonist MRS-1191. Immunohistochemical assays confirmed the presence of A3 receptors at mammalian NMJ. The voltage-gated calcium channel (VGCC) blocker Cd2+, the removal of extracellular Ca2+ and the L-type and P/Q-type VGCC antagonists, nitrendipine and ω-agatoxin IVA, respectively, all prevented inosine-induced inhibition. In the absence of endogenous adenosine, inosine decreased the hypertonic response. The effects of inosine on ACh release were prevented by the Gi/o protein inhibitor N-ethylmaleimide, PKC antagonist chelerytrine and calmodulin antagonist W-7, but not by PKA antagonists, H-89 and KT-5720, or the inhibitor of CaMKII KN-62. Conclusion and Implications Our results suggest that, at motor nerve terminals, inosine induces presynaptic inhibition of spontaneous and evoked ACh release by activating A3 receptors through a mechanism that involves L-type and P/Q-type VGCCs and the secretory machinery downstream of calcium influx. A3 receptors appear to be coupled to Gi/o protein. PKC and calmodulin may be involved in these effects of inosine. © 2013 The British Pharmacological Society.


Sarnachiaro G.O.,Columbia University | Sarnachiaro G.O.,Temple University | Chu S.J.,Columbia University | Sarnachiaro E.,Fundacion Cientifica Buenos Aires | And 2 more authors.
Clinical Implant Dentistry and Related Research | Year: 2016

Purpose: To measure the buccal plate reconstruction of extraction sockets with labial plate dehiscence defects using a bone allograft in combination with an absorbable collagen membrane and a custom-healing abutment at the time of tooth removal. Materials and Methods: Ten patients underwent immediate implant placement and reconstruction of the buccal plate. Cone beam computed tomography (CBCT) was performed preextraction, immediately after bone grafting and implant placement (day 0), and between 6 and 9 months following implant surgery. Measurements were taken at three levels: coronal (L1), middle (L2), and apical (L3) level. Results: Implants placed into sockets with labial plate dehiscence defects demonstrated radiographic reformation of the labial plate dehiscence defect at 6 to 9 months posttreatment. The net gain in labial plate on cone beam computerized tomography (CBCT) in L1 and L2 was 3.0 mm, where 0 mm existed at pretreatment. The minimum amount of labial plate thickness of 2.0 mm was achieved in all treated sites, evaluated radiographically at 6 to 9 months postoperatively, in a single procedure, without flap elevation and maintaining the gingival architecture and satisfactory esthetics. Conclusion: Placing an absorbable membrane, bone graft, and custom-healing abutment at the time of flapless anterior tooth extraction and immediate implant placement into a socket with a labial osseous dehiscence is a viable clinical technique to reconstitute the absence of the labial bone plate. © 2015 Wiley Periodicals, Inc.


Romero J.,CONICET | Muniz J.,CONICET | Tornatore T.L.,CONICET | Holubiec M.,CONICET | And 9 more authors.
Neuroscience Letters | Year: 2014

Perinatal asphyxia represents an important cause of severe neurological deficits including delayed mental and motor development, epilepsy, major cognitive deficits and blindness. However, at the moment, most of the therapeutic strategies were not well targeted toward the processes that induced the brain injury during perinatal asphyxia. Traditionally, experimental research focused on neurons, whereas astrocytes have been more related with the damage mechanisms of perinatal asphyxia. In this work, we propose to review possible protective as well as deleterious roles of astrocytes in the asphyctic brain with the aim to stimulate further research in this area of perinatal asphyxia still not well studied. © 2013 Elsevier Ireland Ltd.


Cosentino J.C.,John F. Kennedy Argentine University
Agora (Brazil) | Year: 2013

This is a critical and comparative reading of the manuscripts of The Ego and the Id. We can read in this work a dissymmetry between the repressed unconscious and the material that remains unknown. We depart from chapter IV, "The two kinds of Drives". As Freud announces, the matters he displays resume thoughts from his writings of 1920. So, starting with the redefinition of Ucs and afterwards, with the reformulation of the idea of Todestrieb, they remain much closer to psychoanalysis than Beyond the pleasure principle. But what he calls, "our speculation" returns in the conclusions of chapter IV about the assumption of the Todestrieb. With the breaking through of the not bound: what are the purposes the Todestrieb serves, when, as he says in 1923, it has its hands free? In 1924 he confirms that not-all the drive is inscribed in the representation. The silence of the drive intervenes: this border, where id speaks, id enjoys and knows nothing.


Catanzaro O.,John F. Kennedy Argentine University | Capponi J.A.,John F. Kennedy Argentine University | Michieli J.,John F. Kennedy Argentine University | Labal E.,John F. Kennedy Argentine University | And 2 more authors.
Peptides | Year: 2013

Diabetic peripheral neuropathy is one the most common complications of diabetes mellitus and frequently results in clinically significant morbidities such as pain, foot ulcers and amputations. The diabetic condition progresses from early functional changes to late, poorly reversible structural changes. The chronic hyperglycemia measured alongside diabetes development is associated with significant damage and failure of various organs. In the present study diabetes was induced in male Wistar rats by a single dose of streptozotocin (STZ) and the association between the BKB1-R and the oxidative stress and Na+-K+ ATPase activity in nervous tissues was analysed. The results showed that the resulting hyperglycemia induced a reduction of the neuronal electrical function integrity and increased oxidative stress in the sciatic nerve homogenates of 30 days diabetic rats. Malondialdehyde (MDA) used as a marker of oxidative stress was elevated whereas Biological Antioxidant Potential (BAP), glutathion (GSH) levels and superoxide dismutase (SOD) activity were decreased. Treatment of the rats 3 days before the end of the 4 week period with the BKB1 antagonist R-954 restored the neuronal activity and significantly attenuated the oxidative stress as shown by the level of the various markers returning close to levels found in control rats. Our results suggest that the BKB1-R subtype is overexpressed in sciatic nerve during the STZ-induced diabetes development as evidenced by inhibitory effects of the BKB1-R antagonist R-954. The beneficial role of BKB 1-R antagonist R-954 for the treatment of diabetic neuropathy is also suggested. © 2013 Elsevier Inc. All rights reserved.


Costa N.E.,John F. Kennedy Argentine University
Vertex (Buenos Aires, Argentina) | Year: 2012

This article is intended to try give light to one of the questions that often reveals to the student of Psychology and Psychiatry on the application of Jungian psychotherapy. How is its praxis? Is it done face to face or does it use a couch? Which are the methods employed? On the basis of Jung's own claims that it defends no doctrine and only describes facts and leaves to everyone the freedom to deal with them, we will find from a fundamentally empirical analytic space. It does not work from a scheme systematized on the basis of a prior theoretical orthodoxy. On the contrary, each patient will be presented as a new challenge to the understanding of the therapist.


Costa N.E.,John F. Kennedy Argentine University
Vertex (Buenos Aires, Argentina) | Year: 2012

Jung conceptualized the collective unconscious as the locus of the myths, the absolutely genuine and primary patterns of thinking and feeling of humanity. The legend of Oedipus or myth of incest is a irrefutable proof of its eternal validity. The present essay is an attempt to take an alternative look to a history that belong to all of us. Situations that are developed in this drama, like the characters involved in it may charge significance as an archetypal and essentially symbolic interpretation.


Catanzaro O.,John F. Kennedy Argentine University | Catanzaro O.,University of Salvador | Labal E.,John F. Kennedy Argentine University | Andornino A.,John F. Kennedy Argentine University | And 3 more authors.
Peptides | Year: 2012

The chronic hyperglycemia measured alongside diabetes development is associated with significant long-term damage and failure of various organs. In the present study it was shown that hyperglycemia induced early and long term increases in nitric oxide (NO) levels, kallikrein activity and vascular capillary permeability measured as plasma extravasation, and decreases of Na/K ATPase activity in diabetic rat retina 4 and 12 weeks after streptozotocin (STZ) injection. Treatment of the animals for 5 consecutive days with a novel selective bradykinin B 1 receptor (BKB 1-R) antagonist R-954 (2 mg/kg s.c) at the end of the 4 and 12 week periods highly reduced NO, kallikrein and capillary permeability and increased Na/K ATPase activity in the retina. These results suggest that the BKB 1-R receptor subtype is over-expressed during the streptozotocin-induced development of diabetes in rat retina as evidenced by the inhibitory effects of the BKB 1-R antagonist R-954 on NO, kallikrein and vascular permeability increases as well as Na/K ATPase decreases. The beneficial role of the BKB 1-R antagonist R-954 for the treatment of the diabetic retinopathy is also suggested. © 2012 Elsevier Inc. All rights reserved.


PubMed | Fundacion Cientifica Buenos Aires, John F. Kennedy Argentine University and Columbia University
Type: Journal Article | Journal: Clinical implant dentistry and related research | Year: 2016

To measure the buccal plate reconstruction of extraction sockets with labial plate dehiscence defects using a bone allograft in combination with an absorbable collagen membrane and a custom-healing abutment at the time of tooth removal.Ten patients underwent immediate implant placement and reconstruction of the buccal plate. Cone beam computed tomography (CBCT) was performed preextraction, immediately after bone grafting and implant placement (day 0), and between 6 and 9 months following implant surgery. Measurements were taken at three levels: coronal (L1), middle (L2), and apical (L3) level.Implants placed into sockets with labial plate dehiscence defects demonstrated radiographic reformation of the labial plate dehiscence defect at 6 to 9 months posttreatment. The net gain in labial plate on cone beam computerized tomography (CBCT) in L1 and L2 was 3.0mm, where 0mm existed at pretreatment. The minimum amount of labial plate thickness of 2.0mm was achieved in all treated sites, evaluated radiographically at 6 to 9 months postoperatively, in a single procedure, without flap elevation and maintaining the gingival architecture and satisfactory esthetics.Placing an absorbable membrane, bone graft, and custom-healing abutment at the time of flapless anterior tooth extraction and immediate implant placement into a socket with a labial osseous dehiscence is a viable clinical technique to reconstitute the absence of the labial bone plate.

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