Duganath N.,JNTUA Oil Technological Research Institute
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014
Flavonoids are one of the active principles isolated from plant sources. These compounds have found to have enormous pharmacological activity, Naringin, a molecule with a wide spectrum of pharmacological activity still has not been used for treatment of any diseases due to its lacking potency. The present study was focused to synthesize various derivatives of naringin and to evaluate their activity. Among the various synthesized compounds, compound Ng, Nn and Nj have shown more potent activity even when compared with the compound. Whereas all other compounds were not potent as standard compound but were found to have increased in potency while comparing with the standard compound. Thus we conclude substitution of the oxygen moiety with the hydrazide to form hydrazones increases the antibacterial activity and presence of electron donating groups further increases the activity.
Bindu N.H.S.,JNTUA Oil Technological Research Institute |
Rubesh Kumar S.,JNTUA Oil Technological Research Institute
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013
The present study was aimed to develop a simple and validated chromatographic method for the estimation of Trimyristin in the API and in poly herbal Ayurvedic formulations by UFLC method, along with stress induced degradation studies of the drug and validate the method as per the ICH guidelines. The analysis was carried out under isocratic conditions using dichloromethane and acetonitrile in the ratio of 95:5 as mobile phase with a flow rate of 1.0 ml/min at 210nm. The method was simple, specific, precise, and accurate with retention times 0.904 and 0.906 minutes for the API and isolated product. The system obeys the Beer's law in the concentration range of 10-90μig/ml. The percentage recovery studies performed showed a percentage recovery of 98.3 -101.6%w/v and found to be linear with a correlation coefficient (r2) of 0.9993. The method was found to be precise with relative standard deviation of less than 2%, detection limit and quantitation limit was estimated to be 4.58μig/ml, 15.27μig/ml respectively. The method was found to be robust even by change in mobile phase ratio of ±5%, change in wavelength and change in flow rate of ±0.1 ml/min. This validated method was sensitive and reproducible enough to be used for routine analysis of Poweromin tablet and Makaradhwaj vatti in time and cost effective manner.
Hima V.,JNTUA Oil Technological Research Institute |
Rubesh Kumar S.,JNTUA Oil Technological Research Institute |
Duganath N.,JNTUA Oil Technological Research Institute |
Devanna N.,JNTUA Oil Technological Research Institute
Der Pharma Chemica | Year: 2013
Phyllanthus Emblica, commonly known as amla used in the ayurvedic preparations of Indian system of medicine. As there are no reported methods for this ayurvedic capsule dosage form of amla, so in this present study, an attempt has been made to develop an analytical method in spectroscopy and TLC for the estimation of ascorbic acid in this formulation. Spectrophotometric estimation of ascorbic acid shows the maximum absorbance at 264nm. Beer's law is obeyed in the concentration range of 1-6 μg/ml. Assay method was developed for the ayurvedic formulation, the percentage purity was found to be 97.68 % w/v. The method was validated as per ICH guidelines and results were statistically calculated. Recovery studies were carried out and the percentage recovery was found to be 99.01-100.70%w/v, the system was found to be linear and the correlation coefficient (r2) was found to be 0.9994, method was found to be precise and the relative standard deviation for intraday and interday was found to be 0.122 and 0.163 respectively, detection and quantification limit was found to be 0.3μg/ml and 1μg/ml respectively. TLC method were carried out for identification of ascorbic acid in standard and ayurvedic formulation by using a solvent system of dichloromethane: acetone: GAA (4:3:3 v/v/v) and the Rf value of ascorbic acid was found to be 0.77. The stress degradation studies were performed for both API and Ayurvedic formulation as per ICH guidelines, the degradation was observed in oxidative, photolytic, hydrolytic degradation under acidic, alkaline conditions and dry heat induced studies.
Thejeswari Y.,JNTUA Oil Technological Research Institute |
Rubesh Kumar S.,JNTUA Oil Technological Research Institute
Research Journal of Pharmacy and Technology | Year: 2013
Curcumin is an ancient medicine to present clinical trials. Curcuminoids natural yellow pigments, the principle constituents of Curcuma longa of Zingiberaceae family comprises of curcumin, demethoxycurcumin and bisdemethoxycurcumin. In the present study, a fast, simple and reliable method for the determination of curcumin present in the API and Ayurvedic formulation haridra capsule by using a single mobile phase for a cost effective method. The effluent was monitored at 419nm. The method was validated according to ICH guidelines various parameters like Specificity, Linearity, Precision, Accuracy, Limit of detection, Limit of quantification, Robustness and System suitability. The method was selected for the determination of curcumin. The system was linear with a correlation coefficient (r2) of 0.9998. RSDs% of intraday and interday precisions were determined to be 0.40 and 0.67 for curcumin. Limit of detection and limit of quantification was found to be as 2.97 and 9 for curcumin. The percentage recovery studies was found to be 99.47 to 102.2%w/v. Stress degradation studies proved that compound showed degradation in conditions like hydrolytic under acidic, alkaline, oxidative, photolytic and dry heat degradation studies for the API as per the ICH guidelines. The simplicity of the method makes it highly valuable for quantification of curcumin in API and Ayurvedic formulations. © RJPT.