JNTUA Oil Technological Research Institute

Ananthapuram, India

JNTUA Oil Technological Research Institute

Ananthapuram, India
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Hima Sai Bindu N.,JNTUA Oil Technological Research Institute | Rubesh Kumar S.,JNTUA Oil Technological Research Institute
International Journal of PharmTech Research | Year: 2013

The herbal plant Myristica fragrans also called as nutmeg, has many uses in Ayurvedic system of medicine. The principle constituent of the fatty oil of Myristica fragrans is a triglyceride named Trimyristin and the principle constituent of essential oil is myristicin.The essential oil part of the nutmeg mostly myristicin was believed to be responsible for the various pharmacological actions of the herb. But to the fact, the triglyceride Trimyristin was reported to have anxiogenic activity which is the principle use of the herbal drug in various polyherbal formulations. So considering the need for the method development a simple and sensitive spectrophotometric method was developed for the determination of Trimyristin in API and in poly herbal Ayurvedic formulations and the method was validated as per the ICH guidelines. Trimyristin was extracted from the seeds of Myristica fragrans by reflux method with hexane as a solvent. The UV wavelength was detected at 222 nm by using the co-solvency method. The stability indicating stress degradation studies was also performed as per the ICH guidelines by using a UV spectroscopic method. The method was simple, specific, precise, and accurate. The system obeyes the Beer's law in the concentration range of 10-60 μg/ml. The percentage purity was found to be 99.0% w/v. The percentage recovery studies performed showed a percentage recovery of 98 -101.7%w/v and found to be linear with a correlation coefficient (r2) of 0.99, method was found to be precise with relative standard deviation of less than 2%, detection limit and quantification limit were estimated to be 0.3μg/ml, 1μg/ml respectively. The stability indicating stress degradation studies showed degradation on subjecting to acidic hydrolysis, basic hydrolysis, oxidative conditions, dry heat and photolytic degradation conditions. This method can be successfully employed for quantitative analysis of Trimyristin in API and in polyherbal formulations.


Subba Reddy G.V.,Sri Krishnadevaraya University | Subba Reddy G.V.,University of South Africa | Rafi M.M.,Sri Krishnadevaraya University | Rubesh Kumar S.,JNTUA Oil Technological Research Institute | And 5 more authors.
3 Biotech | Year: 2016

A novel aerobic gram-negative bacterial strain capable of utilizing 2-hydroxyquinoxaline (2-HQ) as sole source of carbon and energy was isolated from Indian agricultural soil and named as HQ1. Strain HQ1 was identified as Ochrobactrum sp. on the basis of morphology, physico-biochemical characteristics and 16S rRNA sequence analysis. The generation time of Ochrobactrum sp. HQ1 on 2-HQ at log phase is 0.71 h or 42.6 min. The degradation of 2-HQ by HQ1 under various physico-chemical parameters was analysed by HPLC and observed to be optimum with a high inoculum density (1.0 OD) at pH 7–8, temperatures 37–40°C and a high concentration of 2-HQ (500 ppm). Degradation of 2-HQ was also improved when additional nitrogen sources were used and this was attributed to the enhanced growth of the bacterium on the readily available nitrogen sources. Analysis of 2-HQ degradation by GC–MS resulted in elucidation of the degradation pathway for HQ1, a novel observation for aerobic Gram-negative bacteria. These findings are a possible indication of the application of HQ1 in the bioremediation of pesticide/metabolite contamination. © 2016, The Author(s).


Duganath N.,JNTUA Oil Technological Research Institute
Der Pharmacia Lettre | Year: 2014

Flavonoids are one of the active principles isolated from plant sources. These compounds have found to have enormous pharmacological activity, Hesperidin, a molecule with a wide spectrum of pharmacological activity still has not been used for treatment of any diseases due to its lacking potency. The present study was focused to synthesize various derivatives of Hesperidin and to evaluate their activity. Among the various synthesized compounds, compound Hg, Hn and Hj have shown more potent activity even when compared with the compound. Whereas all other compounds were not potent as standard compound but were found to have increased in potency while comparing with the standard compound. Thus we conclude substitution of the oxygen moiety with the hydrazide to form hydrazones increases the antibacterial activity and presence of electron donating groups further increases the activity.


Chandra Sekhar K.B.,JNTUA Oil Technological Research Institute | Jayachandra Reddy P.,P.A. College | Ramesh Y.,P.A. College | Ramesh Y.,Ratnam Institute of Pharmacy | And 4 more authors.
International Journal of Research in Pharmaceutical Sciences | Year: 2015

The objective of the present study was to prepare and evaluate microspheres for the sustained release of Trospi-um Chloride by using various ratio of 1.5%w/v chitosan polymer, 2.5% w/v chitosan polymer, 3.5%w/v chitosan polymer. It is used to treat the Urge urinary incontinence and overactive bladders are common conditions often associated with profound impairment of the health and quality of life of the patient. It mainly acts as the M3 receptor antagonist that is present on sphincter muscle which is present at the urinary bladder opening. Trospium Chloride loaded Chitosan microspheres were prepared by a solvent evaporation method. The resultant micro-spheres were evaluated for average particle size, drug entrapment, In vitro drug release. FTIR, DSC and SEM were used to investigate the physical state of the drug in the microspheres. The mean particle size of the microspheres was influenced by varying drug Polymer ratio while drug loading was dependent on drug: polymer ratio. The entrapment efficiency was 98.23%. The release of drug from the microspheres extended upto 8 to 12hrs. The results of FTIR indicated the stable character of Trospium Chloride loaded Chitosan microspheres and also absence of drug polymer interaction. The Trospium Chloride loaded Chitosan microspheres prepared under optimized conditions showed good sustained release characteristics & were stable under the conditions studied. © JK Welfare & Pharmascope Foundation.


Jagadish R.,Government Science College | Jagadish R.,Government First Grade College | Jagadish R.,Andhra University | Shivaraj Y.,Government Science College | And 2 more authors.
International Journal of Research in Pharmaceutical Sciences | Year: 2015

Iron oxide nanoparticles were prepared by a simple, convenient and cost effective co-precipitation method using starch. The prepared iron oxide nanoparticles were characterized by XRD, SEM, IR and UV techniques. The size of the nanoparticles was calculated from XRD data using Debye-Scherer equation. The average particle size was found to be 11 nm. The electrochemical behaviour of caffeine was studied using glassy carbon electrode (GCE) modified with iron oxide nanoparticles by cyclic voltammetry and differential pulse voltammetry using 0.1M ace-tate buffer solution of pH-7.0. Caffeine gives an oxidation peak at 1.426 V with peak current at -5.6×10-5A for iron oxide NPs modified GCE whereas for unmodified GCE peak potential occurs at 1.426 V with peak current at -5.08 × 10-5A. The peak current intensity significantly increased while the peak potential shifted to lower oxidation poten-tial, compared with the unmodified electrode. The results clearly indicated that the modified electrode exhibited excellent electro catalytic activity towards oxidation of caffeine. © JK Welfare & Pharmascope Foundation.


Duganath N.,JNTUA Oil Technological Research Institute
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

Flavonoids are one of the active principles isolated from plant sources. These compounds have found to have enormous pharmacological activity, Naringin, a molecule with a wide spectrum of pharmacological activity still has not been used for treatment of any diseases due to its lacking potency. The present study was focused to synthesize various derivatives of naringin and to evaluate their activity. Among the various synthesized compounds, compound Ng, Nn and Nj have shown more potent activity even when compared with the compound. Whereas all other compounds were not potent as standard compound but were found to have increased in potency while comparing with the standard compound. Thus we conclude substitution of the oxygen moiety with the hydrazide to form hydrazones increases the antibacterial activity and presence of electron donating groups further increases the activity.


Sandhya Rani K.,JNTUA Oil Technological Research Institute | Bharath Rathna Kumar P.,JNTUA Oil Technological Research Institute | Venu Priya R.,JNTUA Oil Technological Research Institute | Chandra Sekhar K.B.,JNTUA Oil Technological Research Institute
International Journal of Research in Pharmaceutical Sciences | Year: 2015

A simple, specific, rapid, accurate and sensitive RP-HPLC method was developed and validated for the simultane-ous estimation of Lamotrigine and Zonisamide in bulk drug and pharmaceutical tablet dosage form. The separation was achieved by using C18 column (250mm×4.6mm, 5μm particle size), Inertsil ODS using a mobile phase of mixed phosphate buffer (pH 3) adjusted with ortho phosphoric acid: acetonitrile (50:50 v/v), with a flow rate of 1.0 ml/min at an ambient temperature of 30°C and at 232 nm as detection wave length. Rt values (Rt = retention time) of Lamotrigine and Zonisamide were found to be 2.666 min and 3.958 min respectively. Lamotrigine and Zonisamide follow Linearity in the concentration range of 5-30μg/ml with a correlation coefficient of 0.9998 and 0.9997 for Lamotrigine and Zonisamide respectively. Percentage purity of Lamotrigine and Zonisamide were found to be 99.45% and 99.66%w/v respectively. The proposed method has been validated for specificity, linearity, range, accuracy, precision and robustness were within the acceptance limit according to ICH Q2(B) guidelines and the developed method can be employed for determination Lamotrigine and Zonisamide in the bulk and combined pharmaceutical dosage form(tablets). © JK Welfare & Pharmascope Foundation.


Bindu N.H.S.,JNTUA Oil Technological Research Institute | Rubesh Kumar S.,JNTUA Oil Technological Research Institute
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

The present study was aimed to develop a simple and validated chromatographic method for the estimation of Trimyristin in the API and in poly herbal Ayurvedic formulations by UFLC method, along with stress induced degradation studies of the drug and validate the method as per the ICH guidelines. The analysis was carried out under isocratic conditions using dichloromethane and acetonitrile in the ratio of 95:5 as mobile phase with a flow rate of 1.0 ml/min at 210nm. The method was simple, specific, precise, and accurate with retention times 0.904 and 0.906 minutes for the API and isolated product. The system obeys the Beer's law in the concentration range of 10-90μig/ml. The percentage recovery studies performed showed a percentage recovery of 98.3 -101.6%w/v and found to be linear with a correlation coefficient (r2) of 0.9993. The method was found to be precise with relative standard deviation of less than 2%, detection limit and quantitation limit was estimated to be 4.58μig/ml, 15.27μig/ml respectively. The method was found to be robust even by change in mobile phase ratio of ±5%, change in wavelength and change in flow rate of ±0.1 ml/min. This validated method was sensitive and reproducible enough to be used for routine analysis of Poweromin tablet and Makaradhwaj vatti in time and cost effective manner.


Hima V.,JNTUA Oil Technological Research Institute | Rubesh Kumar S.,JNTUA Oil Technological Research Institute | Duganath N.,JNTUA Oil Technological Research Institute | Devanna N.,JNTUA Oil Technological Research Institute
Der Pharma Chemica | Year: 2013

Phyllanthus Emblica, commonly known as amla used in the ayurvedic preparations of Indian system of medicine. As there are no reported methods for this ayurvedic capsule dosage form of amla, so in this present study, an attempt has been made to develop an analytical method in spectroscopy and TLC for the estimation of ascorbic acid in this formulation. Spectrophotometric estimation of ascorbic acid shows the maximum absorbance at 264nm. Beer's law is obeyed in the concentration range of 1-6 μg/ml. Assay method was developed for the ayurvedic formulation, the percentage purity was found to be 97.68 % w/v. The method was validated as per ICH guidelines and results were statistically calculated. Recovery studies were carried out and the percentage recovery was found to be 99.01-100.70%w/v, the system was found to be linear and the correlation coefficient (r2) was found to be 0.9994, method was found to be precise and the relative standard deviation for intraday and interday was found to be 0.122 and 0.163 respectively, detection and quantification limit was found to be 0.3μg/ml and 1μg/ml respectively. TLC method were carried out for identification of ascorbic acid in standard and ayurvedic formulation by using a solvent system of dichloromethane: acetone: GAA (4:3:3 v/v/v) and the Rf value of ascorbic acid was found to be 0.77. The stress degradation studies were performed for both API and Ayurvedic formulation as per ICH guidelines, the degradation was observed in oxidative, photolytic, hydrolytic degradation under acidic, alkaline conditions and dry heat induced studies.


Thejeswari Y.,JNTUA Oil Technological Research Institute | Rubesh Kumar S.,JNTUA Oil Technological Research Institute
Research Journal of Pharmacy and Technology | Year: 2013

Curcumin is an ancient medicine to present clinical trials. Curcuminoids natural yellow pigments, the principle constituents of Curcuma longa of Zingiberaceae family comprises of curcumin, demethoxycurcumin and bisdemethoxycurcumin. In the present study, a fast, simple and reliable method for the determination of curcumin present in the API and Ayurvedic formulation haridra capsule by using a single mobile phase for a cost effective method. The effluent was monitored at 419nm. The method was validated according to ICH guidelines various parameters like Specificity, Linearity, Precision, Accuracy, Limit of detection, Limit of quantification, Robustness and System suitability. The method was selected for the determination of curcumin. The system was linear with a correlation coefficient (r2) of 0.9998. RSDs% of intraday and interday precisions were determined to be 0.40 and 0.67 for curcumin. Limit of detection and limit of quantification was found to be as 2.97 and 9 for curcumin. The percentage recovery studies was found to be 99.47 to 102.2%w/v. Stress degradation studies proved that compound showed degradation in conditions like hydrolytic under acidic, alkaline, oxidative, photolytic and dry heat degradation studies for the API as per the ICH guidelines. The simplicity of the method makes it highly valuable for quantification of curcumin in API and Ayurvedic formulations. © RJPT.

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