Jodhpur, India
Jodhpur, India

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Gehlot H.S.,Jn Vyas University | Tak N.,Jn Vyas University | Kaushik M.,Jn Vyas University | Mitra S.,University of Wisconsin - Milwaukee | And 17 more authors.
Annals of Botany | Year: 2013

Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial. © 2013 The Author 2013.

Gehlot H.S.,Jn Vyas University | Ardley J.,Murdoch University | Tak N.,Jn Vyas University | Tian R.,Murdoch University | And 17 more authors.
Standards in Genomic Sciences | Year: 2016

Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal. © 2016 The Author(s).

Rathore S.,Jn Vyas University | Sherwani M.R.K.,Jn Vyas University
Asian Journal of Chemistry | Year: 2010

In present study, physico-chemical characteristics and fatty acids composition of kakri (Cucumis momordica L.) seeds oil were determined. oil contents, protein contents, moisture content, free fatty acid, saponification values, iodine values, refractive index, specific gravity, unsaponifiable matter and fatty acids composition of oils extracted from seeds were determined. The oil content was 22.22%. The oil consists of 68.16% of unsaturated fatty acids and the main fatty acids are linoleic acid (52.63%), palmitic acid (21.05%), oleic acid (14.33%), stearic acid (10.79%) and linolenic acid (1.20%). The other characteristics of the kakri seeds oil are approximately as follows: protein contents, 16.40%; moisture content, 7.0%; free fatty acid, 3.6%; saponification value, 126; refractive index, 1.479; specific gravity, 0.914 g/cm3; unsaponifiable matter, 2.5% and iodine value, 176.

Acharya S.,Jn Vyas University | Sengar K.S.,Jn Vyas University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Indole-3-Butyric acid (I-3-BA) is a plant hormone in the auxin family and is an ingredient in many commercial horticulture plant rooting product. It is a growth regulating hormone and is biologically and analytically an important molecule. Micellar solubilization of I-3-BA in nonionic and ionic surfactants heteromicroenvironment is monitored by fluorescence and absorption spectral techniques has been reported by the authors. The influence of surfactant, concentration and working experimental conditions on the fluorescence spectra of I-3-BA is thoroughly evaluated and discussed. The increase in fluorescence intensity in micellar media can be attributed to the increase in quantum efficiency suggests that the suspended hydrophobic I-3-BA molecules have been solubilized. The solubilizing action has been supplemented and confirmed by few theoretically calculated spectral parameters like, empirical fluorescence coefficient (kf), quantum yield (φf), molar extinction coefficient (e) and Stokes' shift values.

Mehta R.,Jn Vyas University | Daga K.,Jn Vyas University | Gehlot P.,Jn Vyas University
Asian Journal of Chemistry | Year: 2011

In the present investigation attempts have been made to assess the efficiency of low cost adsorbent material prepared from Datura stramonium for removal of Pb(II) from aqueous solution. Operating variables studied were initial metal ion concentration, pH, temperature and contact time. Equilibrium data fit well with the Langmuir and Freundlich isotherm models. Comprehensive characterization of parameters indicates Datura stramonium to be a good adsorbent for adsorption of Pb(II) to treat wastewaters containing low concentration of the metal.

Acharya S.,Jn Vyas University | Mathur V.,Jn Vyas University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2010

Quinine hydrochloride (QH) is a pharmaceutically important heterocyclic alkaloid derived from the bark of cinchona tree. Micellar solubilization of QH in nonionic and ionic surfactant heteromicroenvironment monitored by fluorescence and absorption spectral techniques has been reported by the authors in this communication. The relatively week fluorescence of QH was significantly enhanced in nonionic micellar media formed by Tween-20 surfactant. The influence of the surfactant, concentration and working experimental conditions on the fluorescence spectra of QH is thoroughly evaluated and discussed. The solubilizing action of the surfactants has been confirmed by the theoretically calculated spectral parameters like, empirical fluorescence coefficient, quantum yield, molar extinction coefficient and Stokes' shift. The authors provide a unique format for the analytical and medicinal application of QH based on micellization of the surfactants which makes them promising drug carriers, employing fluorescence, absorption and light scattering measurements.

Surana D.M.,Jn Vyas University | Parihar S.K.,Jn Vyas University
Journal of Mines, Metals and Fuels | Year: 2013

This paper is based on a study and generic investigation on the slope stability of highwall in Saniem iron ore mine, Goa. For geotechnical details, the results of investigations carried out by various agencies for the mine in the past were reviewed. They were also compared to literature and results for the similar rocks in the Goa region. Based on all these a set of strength parameters was developed. These were validated by having sensitivity analysis of recently failed and existing slopes. Sensitivity analyses were carried out for ultimate pit slopes both in foot wall and hanging wall. For design of slopes at Saniem mine, five models for foot wall and six models for hanging wall were prepared along the two sections. In all the models the height and slope of the benches were 6.0 M and 70 degree, respectively. The width of benches varied from 8 M to 12 M.Based on the analysis of the results the most appropriate ultimate pit slope is the one with bench width of 10 M provided the groundwater is not allowed to form any unconfined aquifer. With 10 M and 12 M wide benches, the ultimate pit slopes are 26 degree and 23 degree, respectively excluding the wider benches.

Godara R.N.,Research Laboratory | Gadi B.R.,Jn Vyas University
Biochemical and Cellular Archives | Year: 2015

A lab experiment was carried out to assess the effect of sodium chloride (75 and 150 mM NaCl) and uniconazole (10 mg/l) on the soluble protein, proline and total soluble sugars content and activities of nitrate reductase (NR), in cotyledonary leaves of Ziziphus mauritiana var. rotundifolia. NaCl stress decreased the soluble protein content and NR activity whereas, proline and total soluble sugars content increased significantly at 4,7 and 9 days after seed sowings (DAS). Treatment of uniconazole with combination of NaCl (75 and 150 mM) alleviates the adverse effect of the NaCl, exhibited increases in NR activity and soluble protein content compared with NaCl treatment. The proline and total soluble sugars contents was more at 9 days after sowing (DAS) with uniconazole alone and in combination of 150 mM NaCl respectively. The NaCl salinity was partially ameliorated by uniconazole might be due to increase in metabolites level (i.e. soluble protein, proline, total soluble sugars) and NR activity.

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