JK AgriGenetics Ltd.

Hyderabad, India

JK AgriGenetics Ltd.

Hyderabad, India
SEARCH FILTERS
Time filter
Source Type

Gupta S.K.,Indian International Crops Research Institute for the Semi Arid Tropics | Rai K.N.,Indian International Crops Research Institute for the Semi Arid Tropics | Singh P.,Indian International Crops Research Institute for the Semi Arid Tropics | Ameta V.L.,Devgen Seeds and Crop Technologies Private Ltd. | And 6 more authors.
Field Crops Research | Year: 2015

Pearl millet has recently emerged as a significant irrigated summer season cereal crop in north-western India. But its flowering coincides with air temperatures of ≥40. °C, leading to reduced seed set and poor grain yield in most of the available hybrids, although a few hybrids with good seed set and high yield potential are widely cultivated. Under a recent initiative to diversify the genetic base of heat tolerant hybrids, field screening of 221 hybrid parental lines (both B- and R-lines), 53 germplasm accessions and 4 improved populations over four-year period revealed large genetic variability in seed set at daily maximum air-temperature of ≥42. °C during flowering. Two locations data on 46 medium maturing genotypes screened during summer 2009 showed that seed set in pearl millet started declining when maximum air temperatures reached 42. °C and decreased in curvilinear fashion to 20 percent at 46. °C. Similar relationship of seed set with minimum and mean temperature was observed with threshold values of 26.4. °C and 34.2. °C, respectively. Similarly, the relationship of percent seed set with vapor pressure deficit (VPD) showed threshold value of 6.2. kPa for maximum VPD, 1.2. kPa for minimum VPD and 3.7. kPa for mean VPD. Seed set on 2 each of heat tolerant and susceptible genotypes fitted well on the seed set-temperature response curve for the maximum, minimum and mean air temperatures. Based on 3 to 4 year field screening (2009-2012), five hybrid seed parents (ICMB 92777, ICMB 05666, ICMB 00333, ICMB 02333 and ICMB 03555) and a germplasm accession IP 19877 with 61 to 69% seed set as compared to 71% seed set in a heat tolerant commercial hybrid 9444 (used as a control) was identified. Intra-population variability for heat tolerance was observed in four populations, and highly heat tolerant progenies from two of them were identified. Evaluation of six hybrid parents under controlled environment (maximum temperature of 43. °C and minimum temperature of 22. °C) revealed boot-leaf stage of pearl millet plant to be more heat sensitive than panicle-emergence stage, and investigations on 6 A-/B-pairs under controlled environment (max. temperature of 44. °C and min. temperature of 22. °C) revealed female reproductive system of pearl millet to be more heat sensitive than pollen. Comparison of 23 hybrids and their parents for seed set at high air temperature (>42. °C) showed heat tolerance as a dominant trait, implying heat tolerance in one parent would be adequate to produce heat tolerant hybrids in pearl millet. Heat tolerant composite developed using identified lines showed high mean seed set under high air temperatures during flowering. © 2014 Elsevier B.V.


Saikumar S.,Barwale Foundation and 3 6 666 | Saikumar S.,JK Agri Genetics Ltd | Varma C.M.K.,Bayer South East Asia Pte Ltd. | Saiharini A.,Barwale Foundation and 3 6 666 | And 4 more authors.
NJAS - Wageningen Journal of Life Sciences | Year: 2016

Understanding response of plants to drought stress is critical for developing drought tolerant genotypes with stable performance under water limiting conditions. A backcross inbred population derived in the background of a popular variety Swarna, was evaluated for yield and yield attributing traits under reproductive stage and shoot morphological traits at seedling stage under drought stress over a period of two years during 2011-2013. Selection intensity varied across seasons and years with varied level of moisture stress. Traits such as flowering (DTF), plant height (PH), spikelet fertility (SF), biomass (BM), harvest index (HI) and grain yield (GY) are drastically affected by drought across seasons. Yield reduction varied from 48.5% to 92.4% over control trials. Chlorophyll content (CC) and canopy temperature (CT) recorded higher under drought rather than control. Yield was highly associated with DTF (-0.29* to -0.76**), SF (0.32** to 0.77**), HI (0.82** to 0.97**) and CT (-0.38**). Heritability is found to be similar for grain yield under both conditions. Genotypes displaying higher SF and HI, lower CT, reduction in flowering delay and low DSI are able to produce better grain yield under drought. High yielding genotypes at reproductive stage stress also showed superior performance under stress at seedling stage. Hence, selection intensity for high yield potential under favorable conditions, drought tolerance to varied level of stress (across seasons) and screening under target population environment had the advantage of identifying stable high yielding genotypes with wider adaption suitable for drought-prone regions, which can significantly benefits the poor farmers relying on rainfed lowland rice. © 2016 Royal Netherlands Society for Agricultural Sciences.


Ramachandran E.,Allahabad Agricultural Institute DU | Ramachandran E.,JK Agrigenetics Ltd. | Bhattacharya S.K.,JK Agrigenetics Ltd. | John S.A.,Allahabad Agricultural Institute DU | And 3 more authors.
Journal of Biotechnology | Year: 2011

The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4-15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carryout the inheritance study of expressed characters in the progeny. © 2011 Elsevier B.V.


Sohrab S.S.,King Abdulaziz University | Kamal M.A.,King Abdulaziz University | Ilah A.,Omar Al-Mukhtar University | Husen A.,Post University | And 2 more authors.
Saudi Journal of Biological Sciences | Year: 2015

Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV. © 2014 The Authors.


PubMed | King Abdulaziz University, Omar Al-Mukhtar University, JK AgriGenetics Ltd. and Post University
Type: Journal Article | Journal: Saudi journal of biological sciences | Year: 2016

Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using C1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV.


Sohrab S.S.,King Abdulaziz University | Azhar E.I.,King Abdulaziz University | Kamal M.A.,King Abdulaziz University | Bhattacharya P.S.,JK AgriGenetics Ltd. | Rana D.,JK AgriGenetics Ltd.
Saudi Journal of Biological Sciences | Year: 2014

Cotton is an important crop and its production is affected by various disease pathogens. Monopartite begomovirus associated betasatellites cause Cotton leaf curl disease (CLCuD) in Northern India. In order to access the occurrence and genetic variability of Cotton leaf curl betasatellites, an extensive field survey was conducted in states of Rajasthan, Punjab and Haryana. We selected the betasatellite sequence for analysis as they are reported as important for disease severity and sequence variability. Based on the field observations, the disease incidence ranged from 30% to 80% during the survey. Full genome and DNA β were amplified from various samples while no amplicon was obtained in some samples. The nucleotide sequence homology ranged from 90.0% to 98.7% with Cotton leaf curl virus (CLCuV), 55.2-55.5% with Bhendi yellow vein mosaic virus, 55.8% with Okra leaf curl virus and 51.70% with Tomato leaf curl virus isolates. The lowest similarity (47.8%) was found in CLCuV-Sudan isolate. Phylogenetic analysis showed that analyzed isolates formed a close cluster with various CLCuV isolates reported earlier. The analysis results show sequence variation in Cotton leaf curl betasatellite which could be the result of recombination. The results obtained by genome amplification and sequence variability indicate that some new variants are circulating and causing leaf curl disease in Rajasthan, Punjab and Haryana. © 2014 The Authors.


Krishna G.,Allahabad University | Krishna G.,JK Agri Genetics Ltd. | Reddy P.S.,JK Agri Genetics Ltd. | Ramteke P.W.,Allahabad University | Bhattacharya P.S.,JK Agri Genetics Ltd.
Plant Cell Reports | Year: 2010

Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in many developing countries. In India, it is the second important food legume contributing to 80% of the global production. Several biotic and abiotic stresses are posing a big threat to its production and productivity. Attempts to address these problems through conventional breeding methods have met with partial success. This paper reviews the chronological progress made in tissue culture through organogenesis and somatic embryogenesis, including the influence of factors such as genotypes, explant sources, and culture media including the supplementation of plant growth regulators. Comprehensive lists of morphogenetic pathways involved in in vitro regeneration through organogenesis and somatic embryogenesis using different explant tissues of diverse pigeon pea genotypes are presented. Similarly, the establishment of protocols for the production of transgenics via particle bombardment and Agrobacterium-mediated transformation using different explant tissues, Agrobacterium strains, Ti plasmids, and plant selectable markers, as well as their interactions on transformation efficiency have been discussed. Future research thrusts on the use of different promoters and stacking of genes for various biotic and abiotic stresses in pigeon pea are suggested. © 2010 Springer-Verlag.


Sohrab S.S.,King Abdulaziz University | Sohrab S.S.,J K Agri Genetics Ltd | Mirza Z.,King Abdulaziz University | Karim S.,King Abdulaziz University | And 4 more authors.
Archives of Phytopathology and Plant Protection | Year: 2013

Leaf curl and yellow vein mosaic viral disease is the major constraint on okra (Abelmoschus esculentus L.) production in India. Amplified fragment sequence of DNA-β showed highest similarity of 91.7% with Bhendi yellow vein mosaic virus-Tamil Nadu (AJ308425, NC_003405) and lowest similarity of 48.5% with OKLCV (NC_004093), whereas coat protein specific amplified sequence showed highest homology with isolate of Madurai, Haryana, Ludhiana and lowest homology of 92% with Mesta yellow vein mosaic Bahraich virus (MYVMBV) (EU360303). The results obtained in the present study confirm that both the viral diseases of okra reported in southern India are caused by a begomovirus associated with DNA-β in which the plants show leaf curl symptoms and never develops yellow vein mosaic and those plants which show yellow vein mosaic, never develops leaf curl symptoms even in the same rows and field. The okra leaf curl is an emerging virus disease in India. © 2013 Copyright Taylor and Francis Group, LLC.


Sohrab S.S.,King Abdulaziz University | Bhattacharya P.S.,JK Agri Genetics Ltd. | Rana D.,JK Agri Genetics Ltd. | Kamal M.A.,King Abdulaziz University | Pande M.K.,JK Agri Genetics Ltd.
Saudi Journal of Biological Sciences | Year: 2015

Tospovirus has emerged as a serious viral pathogen for several crops including tomato. The tomato production is being severely affected worldwide by Tospovirus. Some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. Resistance to this virus has been identified as good source in wild tomato species (Lycopersicum peruvianum). But the introgression of resistance genes into cultivated tomato lines and the development of interspecific hybrid are hampered due to incompatibility, fertilization barriers and embryo abortion. But this barrier has been broken by applying the embryo rescue methods. This study describes the development of interspecific hybrid tomato plants by highly efficient embryo rescue method and screening for Tospovirus resistance. The interspecific hybrid tomato plants were developed by making a cross between wild tomato species (L. peruvianum) and cultivated tomato (Solanum lycopersicum). The immature embryos were cultured in standardized medium and interspecific hybrids were developed from embryogenic callus. The immature embryos excised from 7 to 35. days old fruits were used for embryo rescue and 31. days old embryos showed very good germination capabilities and produced the highest number of plants. Developed plants were hardened enough and shifted to green house. The hybrid nature of interspecific plants was further confirmed by comparing the morphological characters from their parents. The F1, F2 and F3 plants were found to have varying characters especially for leaf type, color of stem, fruits, size, shapes and they were further screened for virus resistance both in lab and open field followed by Enzyme linked Immunosorbant Assay confirmation. Finally, a total of 11 resistant plants were selected bearing red color fruits with desired shape and size. © 2014 The Authors.


PubMed | King Abdulaziz University and JK Agri Genetics Ltd.
Type: Journal Article | Journal: Saudi journal of biological sciences | Year: 2015

Tospovirus has emerged as a serious viral pathogen for several crops including tomato. The tomato production is being severely affected worldwide by Tospovirus. Some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. Resistance to this virus has been identified as good source in wild tomato species (Lycopersicum peruvianum). But the introgression of resistance genes into cultivated tomato lines and the development of interspecific hybrid are hampered due to incompatibility, fertilization barriers and embryo abortion. But this barrier has been broken by applying the embryo rescue methods. This study describes the development of interspecific hybrid tomato plants by highly efficient embryo rescue method and screening for Tospovirus resistance. The interspecific hybrid tomato plants were developed by making a cross between wild tomato species (L. peruvianum) and cultivated tomato (Solanum lycopersicum). The immature embryos were cultured in standardized medium and interspecific hybrids were developed from embryogenic callus. The immature embryos excised from 7 to 35days old fruits were used for embryo rescue and 31days old embryos showed very good germination capabilities and produced the highest number of plants. Developed plants were hardened enough and shifted to green house. The hybrid nature of interspecific plants was further confirmed by comparing the morphological characters from their parents. The F1, F2 and F3 plants were found to have varying characters especially for leaf type, color of stem, fruits, size, shapes and they were further screened for virus resistance both in lab and open field followed by Enzyme linked Immunosorbant Assay confirmation. Finally, a total of 11 resistant plants were selected bearing red color fruits with desired shape and size.

Loading JK AgriGenetics Ltd. collaborators
Loading JK AgriGenetics Ltd. collaborators