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Hyderabad, India

Saikumar S.,Barwale Foundation and 3 6 666 | Saikumar S.,JK AgriGenetics Ltd. | Varma C.M.K.,Bayer South East Asia Pte Ltd | Saiharini A.,Barwale Foundation and 3 6 666 | And 4 more authors.
NJAS - Wageningen Journal of Life Sciences | Year: 2016

Understanding response of plants to drought stress is critical for developing drought tolerant genotypes with stable performance under water limiting conditions. A backcross inbred population derived in the background of a popular variety Swarna, was evaluated for yield and yield attributing traits under reproductive stage and shoot morphological traits at seedling stage under drought stress over a period of two years during 2011-2013. Selection intensity varied across seasons and years with varied level of moisture stress. Traits such as flowering (DTF), plant height (PH), spikelet fertility (SF), biomass (BM), harvest index (HI) and grain yield (GY) are drastically affected by drought across seasons. Yield reduction varied from 48.5% to 92.4% over control trials. Chlorophyll content (CC) and canopy temperature (CT) recorded higher under drought rather than control. Yield was highly associated with DTF (-0.29* to -0.76**), SF (0.32** to 0.77**), HI (0.82** to 0.97**) and CT (-0.38**). Heritability is found to be similar for grain yield under both conditions. Genotypes displaying higher SF and HI, lower CT, reduction in flowering delay and low DSI are able to produce better grain yield under drought. High yielding genotypes at reproductive stage stress also showed superior performance under stress at seedling stage. Hence, selection intensity for high yield potential under favorable conditions, drought tolerance to varied level of stress (across seasons) and screening under target population environment had the advantage of identifying stable high yielding genotypes with wider adaption suitable for drought-prone regions, which can significantly benefits the poor farmers relying on rainfed lowland rice. © 2016 Royal Netherlands Society for Agricultural Sciences.

Sohrab S.S.,King Abdulaziz University | Azhar E.I.,King Abdulaziz University | Kamal M.A.,King Abdulaziz University | Bhattacharya P.S.,JK AgriGenetics Ltd. | Rana D.,JK AgriGenetics Ltd.
Saudi Journal of Biological Sciences | Year: 2014

Cotton is an important crop and its production is affected by various disease pathogens. Monopartite begomovirus associated betasatellites cause Cotton leaf curl disease (CLCuD) in Northern India. In order to access the occurrence and genetic variability of Cotton leaf curl betasatellites, an extensive field survey was conducted in states of Rajasthan, Punjab and Haryana. We selected the betasatellite sequence for analysis as they are reported as important for disease severity and sequence variability. Based on the field observations, the disease incidence ranged from 30% to 80% during the survey. Full genome and DNA β were amplified from various samples while no amplicon was obtained in some samples. The nucleotide sequence homology ranged from 90.0% to 98.7% with Cotton leaf curl virus (CLCuV), 55.2-55.5% with Bhendi yellow vein mosaic virus, 55.8% with Okra leaf curl virus and 51.70% with Tomato leaf curl virus isolates. The lowest similarity (47.8%) was found in CLCuV-Sudan isolate. Phylogenetic analysis showed that analyzed isolates formed a close cluster with various CLCuV isolates reported earlier. The analysis results show sequence variation in Cotton leaf curl betasatellite which could be the result of recombination. The results obtained by genome amplification and sequence variability indicate that some new variants are circulating and causing leaf curl disease in Rajasthan, Punjab and Haryana. © 2014 The Authors.

Sohrab S.S.,King Abdulaziz University | Yasir M.,King Abdulaziz University | El-Kafrawy S.A.,King Abdulaziz University | Mirza Z.,King Abdulaziz University | And 3 more authors.
Plant OMICS | Year: 2015

Okra leaf curl and yellow vein mosaic disease caused by begomovirus and satellite DNA complexes is a major constraint of okra (Abelmoschus esculentus L.) production in India. We performed recombination analysis, in silico structural characterization of ßC1 protein gene from okra leaf curl betasatellites isolated from Hyderabad and discussed the recombination events, hot spots and break points. The results of recombination and in silico structural characterization indicates that the virus associated with Okra leaf curl disease in Southern India could be a variant of Bhendi yellow vein mosaic virus associated betasatellite. Six major and minor inter-specific recombination events, hot spots and breakpoints were identified in Okra leaf curl betasatellites, Hyderabad isolate. The closest major and minor recombination was observed with Bhendi yellow vein betasatellite-Muthupatti and Malvastrum yellow vein betasatellite-China. Recombination was also observed with Cotton leaf curl betasatellite-Rajasthan and Cotton leaf curl Gezira betasatellite-Sudan. The predicted secondary structure indicates a mixed class with helices (29.7%), extended sheets (33.9%) and loops (36.4%).The predicted tertiary model of Okra leaf curl betasatellite has three α helix: one short helix, H1 (37-41) and two long helices, H2 (62-74) & H3 (84-97). This is the first structural characterization for the ßC1 protein gene associated with Okra leaf curl disease.

Sohrab S.S.,King Abdulaziz University | Kamal M.A.,King Abdulaziz University | Ilah A.,Omar Al-Mukhtar University | Husen A.,Post University | And 2 more authors.
Saudi Journal of Biological Sciences | Year: 2015

Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV. © 2014 The Authors.

Ramachandran E.,Allahabad Agricultural Institute Du | Ramachandran E.,JK AgriGenetics Ltd. | Bhattacharya S.K.,JK AgriGenetics Ltd. | John S.A.,Allahabad Agricultural Institute Du | And 3 more authors.
Journal of Biotechnology | Year: 2011

The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4-15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carryout the inheritance study of expressed characters in the progeny. © 2011 Elsevier B.V.

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