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Taheri Sarvtin M.,Jiroft University of Medical science | Taheri Sarvtin M.,Kerman Medical University | Taheri Sarvtin M.,Mazandaran University of Medical Sciences | Shokohi T.,Mazandaran University of Medical Sciences | And 3 more authors.
International Journal of Dermatology | Year: 2014

Background: Psoriasis is an inflammatory skin disease that can considerably affect a patient's quality of life. Environmental and genetic factors, as well as superantigens and toxins from Candida species, may play various roles in the exacerbation and persistence of psoriasis. In the present study, we evaluated candidal colonization and specific humoral responses against Candida albicans in patients with psoriasis. Methods: A total of 100 patients with psoriasis vulgaris and 50 healthy control individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagar Candida medium. Isolated yeast-like fungi were identified using the sequence of the D1/D2 domain of the 26S rRNA gene. Enzyme-linked immunosorbent assays (ELISAs) were used to detect immunoglobulin M (IgM), IgA, and IgG antibodies against C. albicans in sera of patients and healthy individuals. Results: Candida species were isolated from the skin of 15% of patients and 4% of controls and from oral specimens of 60% of patients and 20% of controls. There was a significant difference in candidal colonization between patients and controls (P < 0.05). Serum IgM, IgA, and IgG levels against C. albicans were significantly lower in patients with psoriasis than in controls (P < 0.05). There was no significant association between serum levels of specific antibodies against C. albicans or the frequency of candidal colonization with the clinical severity of the disease (P > 0.05). Conclusions: The results of the present study show a higher rate of candidal colonization in patients with psoriasis in comparison with controls and a reduction in humoral immune responses in patients. © 2014 The International Society of Dermatology. Source


Yahaghi E.,Baqiyatallah Medical Sciences University | Khamesipour F.,Islamic Azad University | Mashayekhi F.,Jiroft University of Medical science | Dehkordi F.S.,Islamic Azad University | And 3 more authors.
BioMed Research International | Year: 2014

From a clinical and epidemiological perspective, it is important to know which genotypes and antibiotic resistance patterns are present in H. pylori strains isolated from salads and vegetables. Therefore, the present investigation was carried out to find this purpose. Three hundred eighty washed and unwashed vegetable samples and fifty commercial and traditional salad samples were collected from Isfahan, Iran. Samples were cultured and those found positive for H. pylori were analyzed using PCR. Antimicrobial susceptibility testing was performed using disk diffusion method. Seven out of 50 (14%) salad and 52 out of 380 (13.68%) vegetable samples harbored H. pylori. In addition, leek, lettuce, and cabbage were the most commonly contaminated samples (30%). The most prevalent virulence genes were oipA (86.44%) and cagA (57.625). VacA s1a (37.28%) and iceA1 (47.45%) were the most prevalent genotypes. Forty different genotypic combinations were recognized. S1a/cagA+/iceA1/oipA+ (33.89%), s1a/cagA+/iceA2/oipA (30.50%), and m1a/cagA+/iceA1/oipA+ (28.81%) were the most prevalent combined genotypes. Bacterial strains had the highest levels of resistance against metronidazole (77.96%), amoxicillin (67.79%), and ampicillin (61.01%). High similarity in the genotyping pattern of H. pylori among vegetable and salad samples and human specimens suggests that vegetable and salads may be the sources of the bacteria. Copyright © 2014 Emad Yahaghi et al. Source


Sepanjnia A.,Jiroft University of Medical science | Eskandari-Nasab E.,Zahedan University of Medical Sciences | Moghadampour M.,Isfahan University of Medical Sciences | Tahmasebi A.,Iran University of Science and Technology | Dahmardeh F.,University of Zabol
Infectious Diseases | Year: 2015

Objective: Cytokines play a critical role in the regulation of the immune response against brucellosis infection, and mediate production of many pro-and anti-inflammatory signals. Transforming growth factor-beta 1 (TGFβ?1), a powerful suppressive cytokine, inhibits macrophage activation and modulates T-cell function, and plays crucial roles in regulation of microbial replication and host responses to brucella. Methods: The association of three polymorphisms in the TGFβ?1 gene ( - 509 C/T [rs1800469],+868 C/T [rs1800470], and+913 G/C [rs1800471]) in promoter, codons 10 and 25, respectively, with brucellosis infection was evaluated. This case-control study was performed on a total of 281 Iranian subjects including 153 patients with active brucellosis and 128 age-and sex-matched healthy individuals as controls. Genotyping for the TGFβ?1 - 509 C/T and+868 C/T variants was performed using tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). Also, the+913 G/C polymorphism was genotyped using an allelespecific PCR. Results: The results demonstrated that the TGFβ?1+868 C/T mutant homozygote genotype (TT vs CC), was a risk factor for developing brucellosis in the co-dominant and recessive models (odds ratio (OR)=2.60, p=0.023; OR=2.602, p=0.014, respectively). Additionally, the diplotype analyses revealed that TGFβ?1 codon 10 and 25 diplotype, TT/GG, was associated with an increased risk of brucellosis (OR=2.49, p=0.038). Other TGFβ?1 variants did not increase the risk of brucellosis infection. Conclusions: Our findings propose that TGFβ?1+868 TT genotype and TT/GG diplotype may confer increased risk of brucellosis in the examined population. © 2015 Informa Healthcare. Source


Sarvtin M.T.,Mazandaran University of Medical Sciences | Sarvtin M.T.,Jiroft University of Medical science | Hedayati M.T.,Mazandaran University of Medical Sciences | Shokohi T.,Mazandaran University of Medical Sciences | HajHeydari Z.,Mazandaran University of Medical Sciences
Archives of Iranian Medicine | Year: 2014

Background: Psoriasis is a common chronic and recurrent inflammatory skin disorder characterized by hyper proliferation of keratinocytes and infiltration of T cells, monocytes/macrophages and neutrophils into dermal and epidermal layers of the skin. The prevalence of cardiovascular disorders in these patients is remarkably higher compared to normal individuals, which seems to be associated with the hyperlipidemia. This study was designed and conducted to investigate the serum lipid profile in psoriatic patients and its association with the severity of disease. Materials and Methods: This case-control study was performed on 50 plaque-type psoriasis patients and 50 healthy individuals as control, matched for age and sex. Blood samples were collected after 14 h fasting. Serum triglyceride, cholesterol and lipoproteins were assayed using the standard kit (made by Pars Among Co. Iran). Results: Certain parameters, including serum triglyceride, cholesterol, low density lipoprotein (LDL), and very low density lipoprotein (VLDL), were significantly higher in the case group compared to the controls (P < 0.001), while high density lipoprotein (HDL) was significantly lower in the former (P < 0.001). In addition, there was a significant relationship between severity of psoriasis and serum lipid profile. Conclusion: The results have revealed the higher plasma level of lipids in psoriatic patients. This may elevate the risk of atherosclerosis, particularly cardiovascular disorders. Therefore, from the epidemiological point of view, screening psoriatic patients, particularly those with severe psoriasis, is recommended. Source


Naderi A.,Kerman Medical University | Nikvarz M.,Jiroft University of Medical science | Arasteh M.,Kerman Special Disease Center | Shokoohi M.,Kerman Medical University
Archives of Iranian Medicine | Year: 2012

Background: Types A and B hemophilia are coagulation disorders associated with many complications. Osteoporosis is a skeletal condition characterized by the decreased density of normally mineralized bone. This study aims to determine the relationship between osteoporosis and hemophilic arthropathy in severe hemophilia patients over the age of 20 years in Kerman, Iran. Methods: We performed a cross-sectional study of bone density among 40 male patients with severe hemophilia. Lumbar spine and femoral bone mineral density (BMDs) were measured using a Dual Energy X-ray Absorptiometry (DEXA) scan. The T-scores for BMDs were computed and values from -2.5 to -1 were considered as osteopenia. Those less than -2.5 were considered to be osteoporotic. Results: About 42% of patients had normal BMD, 50% were osteopenic, and the rest were osteoporotic. The mean BMI, number of arthropathic joints, and the numbers of joint bleeding during the previous year were significantly higher in osteoporotic patients than osteopenic and normal groups (P = 0.05, = 0.003 and = 0.011, respectively). The mean for factor replacement, the number of joint bleeding episodes in the past year, and the number of arthropathic joints were significant independent predictors of both spinal and femoral BMD. Their odds ratios (OR) were 1.29 (factor replacement), 1.17 (numbers of joint bleeding episodes), and 1.73 (number of arthropathic joints), which were significant (P < 0.05). Conclusion: Our results suggest that men with severe hemophilia have reduced BMD. Patients at risk are those with signs of hemophilic arthropathy. Because osteoporosis may complicate the future treatment of patients with hemophilia, screening of patients with hemophilic arthropathy for reduction of BMD and preventive therapies is highly recommended. Source

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