Yusheng Y.,Second Affiliated Hospital |
Chengyue W.,Jinzhou Central Hospital |
Zhiying W.,Second Affiliated Hospital |
Guijun W.,First Affiliated Hospital
Cleft Palate-Craniofacial Journal | Year: 2014
Objective: The purpose of this study was to evaluate the effect of different mechanical forces on the expansion of the palatine suture using transsutural distraction osteogenesis. Methods: A total of 48 dogs were used in this study. The experimental groups were treated with a custom-designed internal distractor. Bone regeneration was determined with x-rays and histology. The computed values underwent statistical analyses using analysis of variance. Results: The maxillary complex was most noticeably advanced with an applied mechanical force of 600 g (20.15 ± 1.36 mm), compared with forces of 400 g (19.88 ± 1.41 mm) and 800 g (2.24 ± 0.93 mm). Immunohistochemical staining showed that the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 fluctuated with different mechanical forces. These changes were statistically significant when 600 g of force was applied within 30 days of distraction (P < .05). Conclusions: Transsutural distraction osteogenesis in the growing dog should be safe and well tolerated in inducing bony lengthening of the maxilla, and the optimal force is 600 x g. Bone morphogenetic protein-2 and bone morphogenetic protein-4 may play an important roles in the signaling pathways that link mechanical forces and biological responses. © Copyright 2014 American Cleft Palate-Craniofacial Association.
Zhao J.,Shenyang University |
Wang X.,Shenyang University |
Xu J.,Liaoning Province People Hospital |
Li N.,Jinzhou Central Hospital |
And 3 more authors.
Acta Biochimica et Biophysica Sinica | Year: 2012
Atherosclerosis is an important pathophysiological basis of atherothrombotic stroke (ATS), and inflammation plays a significant role in atherosclerosis formation. In this study, single-nucleotide polymorphisms (SNPs) in three key inflammation-related genes, 5-lipoxygenase activating protein (ALOX5AP), phosphodiesterase 4D (PDE4D), and interleukin-1α (IL-1α), were investigated to determine their association with ATS in Northern Han Chinese. Six-hundred and eighty-two ATS patients and 598 unrelated controls were recruited. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism and matrix-assisted laser desorption ionization time-of-flight mass spectrometry primer extension. The genotype and allele frequencies of each SNP were statistically analyzed. Risk of ATS was found for the ALOX5AP SG13S114A/T AA genotype (P 0.040) and A allele (P 0.033), PDE4D SNP83C/T TT genotype (P 0.010) and T allele (P 0.008) and SNP219A/G GG genotype (P 0.025) and G allele (P 0.022), and the IL-1α-889C/T T allele (P 0.035). The differences still remained significant after adjustment. The ALOX5AP HapA haplotype was not correlated with ATS (P 0.834), but GCGA represented an at-risk haplotype (P 0.008). Furthermore, the PDE4D AA haplotype at SNP219-220 might be an at-risk haplotype (P 0.013), while GA might be a protective haplotype (P 0.005). The ALOX5AP (SG13S114A/T), PDE4D (SNP83C/T, 219A/G), and IL-1α (-889C/T) SNPs were associated with an increased risk of ATS in Northern Han Chinese. © 2012 The Author 2012. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. © 2012 The Author.
Wang J.-h.,Jinzhou Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2012
BACKGROUND: Range of motion after artificial total knee replacement is the key to evaluate whether the patients are satisfied with the treatment or not, as well as the main outcome measures of functional recovery of joint knee. OBJECTIVE: To analyze the trends in the field of joint activity after artificial total knee replacement and to explore the influence factors of joint activity after artificial total knee replacement. METHODS: A total of 244 research articles are found by searching related literature on joint activity after artificial total knee replacement in CNKI database by the author, with the key words of "Knee", "Artificial joint", "Artificial prosthesis" and "Range of motion" both in Chinese and English, from 2002 to 2011. A total of 211 related articles were included in the final analysis according to inclusion criteria and exclusion criteria. The influence factors were analyzed from the aspects of the joint activity before knee replacement, type of prosthesis and rehabilitation training after replacement. RESULTS AND CONCLUSION: The number of research literature related to joint activity after artificial total knee replacement was gradually increased in CNKI over the past decade among which 2010 is the most productive year (n=38, 18.0%). According to the key words, knee replacement was widely used in the treatment of osteoarthritis disease, but the fund of related research literature was far from being enough. Journal of Clinical Rehabilitative Tissue Engineering Research published the most related articles with the help of the column of hard tissue implants (n=41, 19.4%). The bibliometric analysis provides valuable reference for Chinese medical workers in the field of artificial total knee replacement. There are many factors influencing joint activity after artificial total knee replacement, as well as lots of controversies. Clinicians should continuously improve the treatment techniques and rehabilitation training methods to recover the function of joint knee in patients.
Wang Z.,Jinzhou Central Hospital |
Zhang Z.,Jinzhou Central Hospital
International Eye Science | Year: 2016
AIM: To compare the safety and effectiveness of Schlemm canaloplasty and aqueous outflow pathway reconstruction in the treatment of open angle glaucoma. METHODS: Sixty patients (65 eyes) with primary open angle glaucoma treated from January 2010 to January 2014 in our hospital were selected. The patients were divided into group A (30 patients with 32 eyes) and group B (30 patients with 33 eyes). Group A was given Schlemm canaloplasty and group B was given the aqueous outflow pathway reconstruction. During the follow-up period, the intraocular pressure (IOP) at 1, 3, 6 and 12mo after treatments as well as the kinds of anti-glaucoma drugs and incidence of complications of the two groups were compared. ■RESLUTS: Before treatments, the IOP of the two groups was fairly equal; the IOP at 1, 3, 6 and 12mo after treatments were not significantly different (P<0.05). Before treatments, patients in group A were given 1~4(mean 2.91±1.01) kinds of drugs; those in group B recieved 1~5(mean 3.12±1.28) kinds of drugs. After treatments, the number of anti- glaucoma drugs used in group A was 1~2 (mean 0.92±0.04), that used in group B was 0~1 (mean 0.14±0.01), which was significantly less than that used in group A(P<0.05). In group A there were 19 cases (59%) with hyphema and 4 cases (12%) in group B. The incidence rate of complications in group B was significantly lower than that in group A (P<0.05). CONCLUSION: Compared with Schlemm canaloplasty, the aqueous outflow pathway reconstruction has a fair effect on the reduction of IOP and has fewer complications. It can be regarded as a safe and effective operation in the clinical treatment of open angle glaucoma. Copyright 2016 by the IJO Press.
Hu W.,Jinzhou Central Hospital
Cancer Research and Clinic | Year: 2014
Objective: To investigate the effects of bortezomib on cellular migration and invasion ability in cultured human prostatic cancer cells DU145 and its mechanism. Methods: Transwell method was used to detect cell migration. Invasion was assayed by Matrigel-coated invasion chambers. Expressions of FAK and Tyr397 proteins were analyzed by Western blot. Results: After treated by bortezomib at the concentration of 10, 20 nmol/L for 24 h, the invasion index of DU145 cells were (69.05±10.56) and (52.55±6.98), they were gradually reduced compared with untreated group (81.55±10.56) (P < 0.05). The migration index were (39.35±6.45), (32.05±4.22), they were also reduced compared with untreated group (58.75±5.41) (P < 0.05). The group of treated by bortezomib showed Tyr397 protein expression had been suppressed. However FAK protein had not marked change. Conclusions: FAK is involved in the regulation of cellular migration and invasion function. Bortezomib might inhibit cells migration and invasion function by down regulation of Tyr397 expression.
Liu Q.-F.,Liaoning Medical University |
Yu H.-W.,Jinzhou Central Hospital |
You L.,Liaoning Medical University |
Liu M.-X.,Liaoning Medical University |
And 3 more authors.
Biochemical and Biophysical Research Communications | Year: 2013
Apelin-13 plays an important role in the migration and proliferation of vascular smooth muscle cells (VSMCs); however, the underlying mechanisms are still unclear. Egr-1 is a nuclear transcription factor, which is considered to be the critical initiating factor of the processes of VSMC proliferation and migration. Egr-1 is known to regulate the expression of osteopontin (OPN), which is a marker of the phenotypic modulation that is a necessary condition of VSMC proliferation and migration. We hypothesized that the role of Apelin-13 is mediated via upregulation of Egr-1. To test this hypothesis, we analyzed the effects of Apelin-13 treatment on Egr-1 mRNA and protein expression in A10 rat aortic VSMCs by RT-PCR and Western blotting, respectively. Results showed that, Apelin-13 upregulated the expression of Egr-1. Furthermore, treatment with the extracellular-regulated protein kinase (ERK) inhibitor, PD98059, inhibited the upregulation of Egr-1 by Apelin-13. In addition, this upregulation was inhibited by treatment of VSMCs with the Egr-1 specific deoxyribozyme ED5 (DNAenzyme/10-23 DRz). Furthermore, ED5 treatment was found to significantly inhibit Apelin-13-induced migration and proliferation of VSMCs using transwell and MTT assays, respectively. The evaluation of OPN mRNA and protein expression levels by RT-PCR and Western blot analyses revealed that ED5 treatment also inhibited Apelin-13-induced OPN upregulation. The results of this study indicated that Apelin-13 upregulates Egr-1 via ERK. Furthermore, Apelin-13 induced the proliferation and migration of VSMCs as well as the upregulation of OPN via the upregulation of Egr-1. These results will provide an important theoretical and experimental basis for the control of inappropriate remodeling of vessel walls, and will hopefully lead to the prevention and treatment of vascular remodeling diseases. © 2013.
Tian B.,First Affiliated Hospital of Liaoning Medical College |
Zhang Y.,Jinzhou Central Hospital |
Li N.,Jinzhou Central Hospital
Cancer Biotherapy and Radiopharmaceuticals | Year: 2013
Objective: To study the expression and regulatory effects of CD146 protein in colorectal cancer and the correlation between CD146 protein expression and the prognosis of colorectal cancer. Materials and Methods: The CD146 protein level was detected by immunohistochemistry staining. The relationship between CD146 expression and clinicopathological parameters of colorectal cancer was determined. Results: It was observed that 216 (20.00%) of the 1080 cases positively expressed CD146 protein. Univariate analyses indicated that CD146 expression was related to histological grade, Duke's stage, and liver metastasis (p=0.001, 0.001, and 0.001, respectively). Spearman correlation analysis showed that CD146 expression has line correlation to histological grade, Duke's stage, and liver metastasis (p=0.02, 0.01 and 0.001, respectively). After multivariate analysis, Duke's stage and CD146 were related to liver metastasis (p=0.01 and 0.001, respectively). In the Cox regression test, histological grade, Duke's stage, and CD146 were detected as the independent prognostic factors (p=0.045, 0.01, and 0.001, respectively). Conclusions: CD146 protein may be a potential biomarker for the postoperative liver metastasis of colorectal cancer. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
Hu Z.,Liaoning Medical University |
Gu Z.,Liaoning Medical University |
Sun M.,Liaoning Medical University |
Zhang K.,Liaoning Medical University |
And 3 more authors.
Journal of Surgical Research | Year: 2015
Background Sepsis is characterized as a systemic inflammatory response syndrome during infection, which can result in multiple organ dysfunction and death. Ursolic acid (UA), a pentacyclic triterpene acid, has been reported to have potent anti-inflammatory and antioxidant properties. The aim of this study was to detect the possible protective effects of UA on sepsis-evoked acute lung injury. Materials and methods A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. Rats were injected intraperitoneally with UA (10 mg/kg) after CLP, and then the survival was determined twice a day for 4 d. The protective effects of UA on CLP-induced acute lung injury were assayed at 24 h after CLP. Results The results revealed that UA treatment markedly improved the survival of septic rats, and attenuated CLP-induced lung injury, including reduction of lung wet/dry weight ratio, infiltration of leukocytes and proteins, myeloperoxidase activity, and malondialdehyde content. In addition, UA significantly decreased the serum levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β, inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2 in the lung, which are involved in the productions of nitric oxide and prostaglandin E2. Conclusions These findings indicate that UA exerts protective effects on CLP-induced septic rats. UA may be a potential therapeutic agent against sepsis. © 2015 Elsevier Inc. All rights reserved.
Tian B.,Liaoning Medical College |
Zhang Y.,Jinzhou Central Hospital |
Zhang J.,Liaoning Medical College
Tumor Biology | Year: 2014
The objective of this study is to investigate the expression level of periostin in cancer stem cells as well as in the glioma tissues and the relationship between periostin expression and clinical and pathological characteristics and prognosis of gliomas. ESA+/CD133+/lin- tumor cells were selected by flow cytometry from glioma tissues, and the periostin expression in ESA+/CD133+/lin- tumor cells and non-ESA+/CD133+/lin- tumor cells was detected by quantitative real-time polymerase chain reaction (RT-PCR) and Western blot analysis. The expression status of periostin in glioma tissues was analyzed by immunohistochemistry staining, and the relationship between periostin and clinicopathological parameters of gliomas was determined. It showed that periostin is expressed higher in ESA+/CD133+/lin- tumor cells compared to non-ESA+/CD133+/lin- tumor cells in both mRNA and protein levels. One hundred eighteen (37.82 %) glioma patients were observed with highly expressed periostin protein in immunohistochemistry. Moreover, we observed that the expression of periostin protein was related to Karnofsky performance scale score (KPS), extent of resection, Ki67, and WHO grade of gliomas in universal analysis (P=0.008, 0.045, 0.001, and 0.001, respectively). However, only WHO grade was identified to be related to periostin expression in gliomas after multivariate analysis. After survival analysis, the cases with highly expressed periostin protein attained a significantly poorer postoperative disease-specific survival and distant metastasis than those with none/low expressed periostin protein (P=0.001 and 0.002). In the Cox regression test, KPS, extent of resection, Ki67, WHO grade, and periostin were detected as the independent prognostic factors (P=0.008, 0.007, 0.032, 0.001, and 0.001, respectively). Periostin can be an important prognostic marker for gliomas, which may present a new therapeutic target for glioma patients. © 2014 International Society of Oncology and BioMarkers (ISOBM).
Jiang W.,Liaoning Medical University |
Wang Z.,Dalian Medical University |
Jiang Y.,Liaoning Medical University |
Lu M.,Liaoning Medical University |
Li X.,Jinzhou Central Hospital
Pharmacology | Year: 2015
Aims: Parkinson's disease (PD) is a neurodegenerative disorder characterized by loss of dopaminergic neurons in the substantia nigra (SN) and diminished dopamine levels in the striatum. Accumulating evidence supported that ginsenoside Rg1, the major pharmacologically active compound of ginseng, has a wide range of neurotrophic and neuroprotective effects under physiological and pathological conditions. Although Rg1 administration protects dopaminergic neurons in a rat model of PD, it is unclear if Rg1 treatment ameliorates motor function in PD. Methods: Using the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) that causes dopaminergic neurodegeneration, we investigated the effect of Rg1 on 3 tests of motor behaviors in mice: the accelerating rotarod, wire suspension and pole tests. Results: The results showed that Rg1 treatment (10 mg/kg, i.p.) succeeded in restoring motor functions to physiological level in MPTP-treated mice. Importantly, these behavioral ameliorations were accompanied by an attenuation of the MPTP-induced loss of dopaminergic neurons in the SN and striatum. Conclusions: These findings indicate that Rg1 can significantly rescue the deficit of motor function in mice model of PD, and suggest that Rg1 may be a potential therapeutic agent against PD and related disorders. © 2015 S. Karger AG, Basel.