Central Hospital of Jinzhou

Jinzhou, China

Central Hospital of Jinzhou

Jinzhou, China
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Li X.,Liaoning Medical University | Li X.,Central Hospital of Jinzhou | Zhang D.,Liaoning Medical University | Zheng J.-M.,Liaoning Medical University | And 2 more authors.
Chinese Pharmacological Bulletin | Year: 2013

Aim: To investigate the effects of urocortin (UCN) on hemodynamics and the remodeling of myocardium, and its mechanism in spontaneous hypertensive rat (SHR). Methods: UCN (1 μg·kg-1·d-1, 3 μg·kg-1·d-1, 6 μg·kg -1·d-1) was intravenously injected daily for 2 weeks and its effects on hemodynamics in spontaneous hypertensive rat were observed. The whole-cell patch clamp technique was used to compare the effects of UCN on left myocardial cell's L-type calcium channel. The flow cytometry method was used to test the content of fluorescence calcium in myocardium. Results: UCN improved the systolic and diastolic function of SHR, obviously decreased the AMP, LVSP, LVEDP (P < 0.01), and increased +dp/dtmax and -dp/dtmax (P < 0.01), improved the hymodynamics for hypertension in SHR. UCN could inhibit the opening of L-type calcium channel and decrease the electric current of cardiamyocytes (P < 0.01). Further more, UCN could decrease the contents of fluorescence calcium in SHR myocardium (P < 0.01). However, AST, the corticotropin-regulating receptor's antagonist (CRF2R), could reverse the UCN's inhibitory effects (P < 0.01). Conclusion: UCN plays an role in treating hypertension via CRF2 receptor and inhibiting the myocardial calcium channel.

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