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Jinzhou, China

Wang J.-h.,Jinzhou Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2012

BACKGROUND: Range of motion after artificial total knee replacement is the key to evaluate whether the patients are satisfied with the treatment or not, as well as the main outcome measures of functional recovery of joint knee. OBJECTIVE: To analyze the trends in the field of joint activity after artificial total knee replacement and to explore the influence factors of joint activity after artificial total knee replacement. METHODS: A total of 244 research articles are found by searching related literature on joint activity after artificial total knee replacement in CNKI database by the author, with the key words of "Knee", "Artificial joint", "Artificial prosthesis" and "Range of motion" both in Chinese and English, from 2002 to 2011. A total of 211 related articles were included in the final analysis according to inclusion criteria and exclusion criteria. The influence factors were analyzed from the aspects of the joint activity before knee replacement, type of prosthesis and rehabilitation training after replacement. RESULTS AND CONCLUSION: The number of research literature related to joint activity after artificial total knee replacement was gradually increased in CNKI over the past decade among which 2010 is the most productive year (n=38, 18.0%). According to the key words, knee replacement was widely used in the treatment of osteoarthritis disease, but the fund of related research literature was far from being enough. Journal of Clinical Rehabilitative Tissue Engineering Research published the most related articles with the help of the column of hard tissue implants (n=41, 19.4%). The bibliometric analysis provides valuable reference for Chinese medical workers in the field of artificial total knee replacement. There are many factors influencing joint activity after artificial total knee replacement, as well as lots of controversies. Clinicians should continuously improve the treatment techniques and rehabilitation training methods to recover the function of joint knee in patients. Source

Zhao J.,Shenyang University | Wang X.,Shenyang University | Xu J.,Liaoning Province People Hospital | Li N.,Jinzhou Central Hospital | And 3 more authors.
Acta Biochimica et Biophysica Sinica | Year: 2012

Atherosclerosis is an important pathophysiological basis of atherothrombotic stroke (ATS), and inflammation plays a significant role in atherosclerosis formation. In this study, single-nucleotide polymorphisms (SNPs) in three key inflammation-related genes, 5-lipoxygenase activating protein (ALOX5AP), phosphodiesterase 4D (PDE4D), and interleukin-1α (IL-1α), were investigated to determine their association with ATS in Northern Han Chinese. Six-hundred and eighty-two ATS patients and 598 unrelated controls were recruited. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism and matrix-assisted laser desorption ionization time-of-flight mass spectrometry primer extension. The genotype and allele frequencies of each SNP were statistically analyzed. Risk of ATS was found for the ALOX5AP SG13S114A/T AA genotype (P 0.040) and A allele (P 0.033), PDE4D SNP83C/T TT genotype (P 0.010) and T allele (P 0.008) and SNP219A/G GG genotype (P 0.025) and G allele (P 0.022), and the IL-1α-889C/T T allele (P 0.035). The differences still remained significant after adjustment. The ALOX5AP HapA haplotype was not correlated with ATS (P 0.834), but GCGA represented an at-risk haplotype (P 0.008). Furthermore, the PDE4D AA haplotype at SNP219-220 might be an at-risk haplotype (P 0.013), while GA might be a protective haplotype (P 0.005). The ALOX5AP (SG13S114A/T), PDE4D (SNP83C/T, 219A/G), and IL-1α (-889C/T) SNPs were associated with an increased risk of ATS in Northern Han Chinese. © 2012 The Author 2012. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. © 2012 The Author. Source

Yusheng Y.,Liaoning Medical College | Chengyue W.,Jinzhou Central Hospital | Zhiying W.,Liaoning Medical College | Guijun W.,Liaoning Medical College
Cleft Palate-Craniofacial Journal | Year: 2014

Objective: The purpose of this study was to evaluate the effect of different mechanical forces on the expansion of the palatine suture using transsutural distraction osteogenesis. Methods: A total of 48 dogs were used in this study. The experimental groups were treated with a custom-designed internal distractor. Bone regeneration was determined with x-rays and histology. The computed values underwent statistical analyses using analysis of variance. Results: The maxillary complex was most noticeably advanced with an applied mechanical force of 600 g (20.15 ± 1.36 mm), compared with forces of 400 g (19.88 ± 1.41 mm) and 800 g (2.24 ± 0.93 mm). Immunohistochemical staining showed that the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 fluctuated with different mechanical forces. These changes were statistically significant when 600 g of force was applied within 30 days of distraction (P < .05). Conclusions: Transsutural distraction osteogenesis in the growing dog should be safe and well tolerated in inducing bony lengthening of the maxilla, and the optimal force is 600 x g. Bone morphogenetic protein-2 and bone morphogenetic protein-4 may play an important roles in the signaling pathways that link mechanical forces and biological responses. © Copyright 2014 American Cleft Palate-Craniofacial Association. Source

Liu Q.-F.,Liaoning Medical University | Yu H.-W.,Jinzhou Central Hospital | You L.,Liaoning Medical University | Liu M.-X.,Liaoning Medical University | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2013

Apelin-13 plays an important role in the migration and proliferation of vascular smooth muscle cells (VSMCs); however, the underlying mechanisms are still unclear. Egr-1 is a nuclear transcription factor, which is considered to be the critical initiating factor of the processes of VSMC proliferation and migration. Egr-1 is known to regulate the expression of osteopontin (OPN), which is a marker of the phenotypic modulation that is a necessary condition of VSMC proliferation and migration. We hypothesized that the role of Apelin-13 is mediated via upregulation of Egr-1. To test this hypothesis, we analyzed the effects of Apelin-13 treatment on Egr-1 mRNA and protein expression in A10 rat aortic VSMCs by RT-PCR and Western blotting, respectively. Results showed that, Apelin-13 upregulated the expression of Egr-1. Furthermore, treatment with the extracellular-regulated protein kinase (ERK) inhibitor, PD98059, inhibited the upregulation of Egr-1 by Apelin-13. In addition, this upregulation was inhibited by treatment of VSMCs with the Egr-1 specific deoxyribozyme ED5 (DNAenzyme/10-23 DRz). Furthermore, ED5 treatment was found to significantly inhibit Apelin-13-induced migration and proliferation of VSMCs using transwell and MTT assays, respectively. The evaluation of OPN mRNA and protein expression levels by RT-PCR and Western blot analyses revealed that ED5 treatment also inhibited Apelin-13-induced OPN upregulation. The results of this study indicated that Apelin-13 upregulates Egr-1 via ERK. Furthermore, Apelin-13 induced the proliferation and migration of VSMCs as well as the upregulation of OPN via the upregulation of Egr-1. These results will provide an important theoretical and experimental basis for the control of inappropriate remodeling of vessel walls, and will hopefully lead to the prevention and treatment of vascular remodeling diseases. © 2013. Source

Objective: To investigate the effects of bortezomib on cellular migration and invasion ability in cultured human prostatic cancer cells DU145 and its mechanism. Methods: Transwell method was used to detect cell migration. Invasion was assayed by Matrigel-coated invasion chambers. Expressions of FAK and Tyr397 proteins were analyzed by Western blot. Results: After treated by bortezomib at the concentration of 10, 20 nmol/L for 24 h, the invasion index of DU145 cells were (69.05±10.56) and (52.55±6.98), they were gradually reduced compared with untreated group (81.55±10.56) (P < 0.05). The migration index were (39.35±6.45), (32.05±4.22), they were also reduced compared with untreated group (58.75±5.41) (P < 0.05). The group of treated by bortezomib showed Tyr397 protein expression had been suppressed. However FAK protein had not marked change. Conclusions: FAK is involved in the regulation of cellular migration and invasion function. Bortezomib might inhibit cells migration and invasion function by down regulation of Tyr397 expression. Source

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