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Jinzhou, China

Wang Z.,Jinzhou | Ren X.-Q.,Medical Team | Wei D.-X.,Jinzhou Center Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Although medications can reduce and delay the progression of Alzheimer’s disease to a certain extent, but the effect is not obvious. Therefore, cell replacement therapy is a new attempt and exploration for the treatment of Alzheimer’s disease. OBJECTIVE: To study the improvement in learning and memory abilities of rats with Alzheimer’s disease after transplantation of bone marrow mesenchymal stem cells. METHODS: Alzheimer’s disease model was induced by injecting beta-amyloid 1-40 protein into the bilateral hippocampi. The bone marrow mesenchymal stem cells were then implanted into the hippocampus. After 4 weeks, the spatial cognition and memory ability of rats were evaluated with Morris water maze. BrdU and NF, GFAP immunofluorescence double-staining was adopted to test the differentiation and survival of implanted bone marrow mesenchymal stem cells. Western blot and immunohistochemistry methods were used to detect the expression of beta-amyloid protein in the cortex and hippocampus. RESULTS AND CONCLUSION: Compared with the model group, the escape latency of hidden platform was shortened significantly in the stem cell transplantation group (P < 0.01), while the number of crossing the platform was increased significantly (P < 0.05). The BrdU/NSE and BrdU/GFAP double staining positive cells could be found around the bilateral hippocampi. Western blot result showed the beta-amyloid protein expression decreased obviously after transplantation of bone marrow mesenchymal stem cells (P < 0.05). These findings indicate that transplanted bone marrow mesenchymal stem cells can survive and differentiate in the brain of rats, which can improve the learning and memory abilities of Alzheimer’s disease rats. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Song H.,Jinzhou Center Hospital | Ren X.-Q.,Jinzhou | Wei D.-X.,Jinzhou Center Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Hydroxyapatite materials have similar spatial structure and hardness to the natural bone matrix, but due to the imperfect early technology, there are some problems in the pore and degradation. OBJECTIVE: To compare the effects of nano-hydroxyapatite materials and hydroxyapatite materials in the repair of disconnected defects of the tibia in rats. METHODS: Thirty-six Sprague-Dawley rats were randomized into four groups, and a 5 mm disconnected defect model was established in the left tibia. Bone marrow mesenchymal stem cells/nano-hydroxyapatite complex was implanted into the experimental group, bone marrow mesenchymal stem cells/hydroxyapaptite complex into the control group, and bone marrow mesenchymal stem cells into the cell group. There was no treatment in the blank control group. At 2, 8, 12 weeks after implantation, X-ray measurement, hematoxylin-eosin staining, western blot assay were used for observation of bone repair, osteogenesis, and expression of type I collagen in the repair area. RESULTS AND CONCLUSION: After 12 weeks of implantation, in the experimental group, the newborn calluses covered the whole defect region and formed visible bridge connections, the scaffold was degraded mostly, a great amount of osteoid tissues were visible to form the bone trabecula with lamellar bone structure, and there were a lot of type I collagens; in the control group, the number of calluses in the defect area was increased, poor integration was found at the broken end, bone sclerosis developed, the scaffold was not completely degraded, bone trabecula and lamellar bone formed, and a large amount of type I collagen was visible but lower than that in the experimental group; in the cell group and blank control group, no obvious bone regeneration was seen, and the expression of type I collagen was weak. These findings suggest that nano-hydroxyapatite is better than hydroxyapatite to promote bone regeneration. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Jiang S.,Jinzhou Center Hospital | Dong S.-C.,Jinzhou Center Hospital | Wei D.-X.,Jinzhou Center Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Bone marrow stromal stem cells transplanted into infracted cardiac tissue can inhibit and reduce myocardial apoptosis, but whether this effect is correlated with improvement in cardiac function is still unclear. OBJECTIVE: To study the early effect of bone marrow stromal stem cells transplanted into the ischemic myocardium on the cardiac function. METHODS: Models of acute myocardial infarction were established by ligation of the left anterior descending branch, while no ligation was done in the sham group. In the transplantation group, rat bone marrow stromal stem cells (0.1 mL, 2x106) were injected into five sites on the edge of infarcted myocardial tissues at 30 minutes after myocardial infarction. In the sham group and model group, the same volume of normal saline was injected into the myocardial tissues. Three days after cell transplantation, hemodynamic monitoring, echocardiography, TUNEL assay were employed to detect myocardial apoptosis. RESULTS AND CONCLUSION: At 3 days after cell transplantation, myocardial apoptosis was more evident in the infarct and ischemic zones of the model group than the sham group; the number of apoptotic myocardial cells was significantly lower in the infarct and ischemic zones of the transplantation group than the model group. Compared with the sham group, the mean arterial blood pressure and left ventricular systolic pressure were significantly reduced, the left ventricular end diastolic pressure was increased, and the left ventricular ejection fraction and shortened fraction were significantly lowered in the model and transplantation groups (P < 0.05), but there was no difference between the model and transplantation groups (P > 0.05). These findings indicate that myocardial apoptosis can be reduced but the cardiac function cannot be improved in acute myocardial infarction rats at early stage after bone marrow stromal stem cells transplantation. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All Rights Reserved. Source

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