Cui X.,Fudan University |
Hong J.,Fudan University |
Hong J.,Xiamen University |
Hong J.,Institutes of Brain Science |
And 7 more authors.
Optometry and Vision Science | Year: 2014
Purpose. To investigate the features of corneal epithelial thickness topography with Fourier-domain optical coherence tomography (OCT) in dry eye patients. Methods. In this cross-sectional study, 100 symptomatic dry eye patients and 35 normal subjects were enrolled. All participants answered the ocular surface disease index questionnaire and were subjected to OCT, corneal fluorescein staining, tear breakup time, Schirmer 1 test without anesthetic (S1t), and meibomian morphology. Several epithelium statistics for each eye, including central, superior, inferior, minimum, maximum, minimum - maximum, and map standard deviation, were averaged. Correlations of epithelial thickness with the symptoms of dry eye were calculated. Results. The mean (+SD) central, superior, and inferior corneal epithelial thickness was 53.57 (+3.31) μm, 52.00 (+3.39) μm, and 53.03 (+3.67) μmin normal eyes and 52.71 (+2.83) μm, 50.58 (+3.44) μm, and 52.53 (+3.36) μmin dry eyes, respectively. The superior corneal epitheliumwas thinner in dry eye patients compared withnormal subjects (p=0.037),whereas central and inferior epitheliumwere not statistically different. In the dry eye group, patients with higher severity grades had thinner superior (p = 0.017) and minimum(p < 0.001) epithelial thickness, more wide range (p = 0.032), and greater deviation (p = 0.003). The average central epithelial thickness hadno correlation with tear breakup time, S1t, or the severityofmeibomian glands,whereas average superior epithelial thickness positively correlated with S1t (r = 0.238, p = 0.017). Conclusions. Fourier-domain OCT demonstrated that the thickness map of the dry eye corneal epithelium was thinner than normal eyes in the superior region. In more severe dry eye disease patients, the superior and minimum epithelium was much thinner, with a greater range of map standard deviation. Copyright © American Academy of Optometry.
Luo Q.,Jinshan Hospital |
Luo Q.,Fudan University |
Chen Y.,Jinshan Hospital |
Chen Y.,Fudan University
Clinical Interventions in Aging | Year: 2016
Long noncoding RNAs (lncRNAs) are typically defined as transcripts longer than 200 nucleotides. lncRNAs can regulate gene expression at epigenetic, transcriptional, and posttranscriptional levels. Recent studies have shown that lncRNAs are involved in many neurological diseases such as epilepsy, neurodegenerative conditions, and genetic disorders. Alzheimer’s disease is a neurodegenerative disease, which accounts for >80% of dementia in elderly subjects. In this review, we will highlight recent studies investigating the role of lncRNAs in Alzheimer’s disease and focus on some specific lncRNAs that may underlie Alzheimer’s disease pathophysiology and therefore could be potential therapeutic targets. © 2016 Luo and Chen.
Wu X.,Huazhong University of Science and Technology |
Wang Q.K.,Huazhong University of Science and Technology |
Wang Q.K.,Cleveland Clinic |
Gui L.,Huazhong University of Science and Technology |
And 9 more authors.
Journal of Huazhong University of Science and Technology - Medical Science | Year: 2010
Even though mutations in LMNA have been reported in patients with typical dilated cardiomyopathy (DCM) and atrioventricular block (AVB) previously, the purpose of this study was to disclose this novel genetic abnormality in one Chinese family with the atypical phenotype of progressive AVB followed by DCM with normal QRS interval. Genome-wide linkage analysis mapped the AVB gene in this family to a marker at chromosome 1q21.2, where the LMNA gene was located. Direct DNA sequence analysis revealed a heterozygous G to A transition at nucleotide 244 in exon 1 of LMNA, which resulted in an E82K mutation. The E82K mutation co-segregated with all affected individuals in the family, and was not present in 200 normal controls. Further clinical evaluation of mutation carriers showed that 5 of 6 AVB patients exhibited mild DCM with a late onset of age in the fourth and fifth decades. Ejection fractions were documented in 5 patients with DCM, but 4 showed a normal value of ≥50%. Echocardiography showed that atrial dilatation occurred earlier than ventricular dilatation in the patients. This study suggests that progressive AVB with normal QRS interval and accompanying DCM at later stages may represent a distinct type of DCM. The molecular mechanism by which the E82K mutation causes AVB as the prominent phenotype in DCM may be a focus of future studies. © 2010 Huazhong University of Science and Technology and Springer Berlin Heidelberg.
Li W.,Jinshan Hospital |
Wang X.,Fudan University |
Chen R.,Affiliated Hospital of Xuzhou Medical University |
Zhu H.,Affiliated Hospital of Xuzhou Medical University |
And 2 more authors.
Journal of Surgical Research | Year: 2012
Background: Co-stimulatory molecules are pivotal for T cell activation. It is increasingly recognized that programmed death ligand 1 (PD-L1) is a novel co-stimulatory molecule, which raises the question as to whether PD-L1 regulates T cell responses. This study aimed to investigate the inhibitory effects of PD-L1 on T cell activation. Materials and Methods: We constructed a transgenic vector containing the complete PD-L1 gene, which interacts with the inhibitory receptor PD-1 in T cell-mediated immune activation. Donor dendritic cells (DCs) derived from C57BL/6 mice were transfected with PD-L1 and mixed with allogeneic, recipient T cells from BALB/c mice. The T cell activation was determined by the MTT assay and T cell proliferation was determined using carboxyfluoroscein succinimidyl ester (CFSE)-labeling following in vitro mixed leukocyte reactions. Results: The expression of PD-L1 protein in PD-L1-transfected DCs was 47.97% ± 1.06%, compared with 4.66% ± 0.76% and 5.30% ± 0.60% in blank and negative controls, respectively (P < 0.05). PD-L1 protein was effectively expressed in DCs. Furthermore, in DCs stably transfected with PD-L1, T cell activation was significantly suppressed and T cell proliferation rate was decreased by 35% compared with untransfected DCs (P < 0.05). Conclusion: PD-L1 delivers an immunoinhibitory signal, suppressing T cell activation. Overexpression of PD-L1 signaling induces tolerance, which presents a promising immunotherapeutic approach for long-term graft acceptance. © 2012 Elsevier Inc. All rights reserved.
Di Y.,Jinshan Hospital |
Di Y.,Fudan University |
Lu N.,Jinshan Hospital |
Li B.,Jinshan Hospital |
And 4 more authors.
Current Eye Research | Year: 2013
Aims: To investigate the effect of prolonged flickering illumination exposure on the growth of the guinea pig eye. Methods: Thirty-six 2-week-old guinea pigs were randomized to one of the three treatment groups (n=12 for each). Two strobe-reared groups were raised with a duty diurnal cycle of 50 % at a flash rate of 0.5Hz and 5Hz respectively. Illumination intensity varied between the minimum-maximum light levels of 0-600lux during each cycle. The control group was exposed to steady 300lux illumination. All animals underwent refraction and biometric measurements prior to and after 2,4,6,8,10 and 12 weeks of treatment. Finally, flash electroretinograms were compared, and retinal microstructures were examined. Results: There was a significant correlation between refractive errors and axial eye elongation, with myopia increasing between 1.5 and 3.4D per mm eye elongation. After 12 weeks of treatment, the animals raised in 0.5Hz flickering light were 5.5±0.4D more myopic than the group raised in continuous illumination, followed by the group raised at 5Hz flicker light which was about 2.2±1.3D more myopic. In animals raised in flickering light of 5 or 0.5Hz for 12 weeks, the implicit time of the a-wave was delayed by 4 and 8.5ms, respectively. At this time, the outer segment disc membranes were found deformed and detached. Conclusion: Chronic exposure to 0.5 and 5Hz temporally modulated illumination induces electrophysiological and histological changes in retinal activities that alter the emmetropization of the guinea pig eye. © 2013 Informa Healthcare USA, Inc.
Chu D.-J.,Jinshan Hospital |
Guo S.-G.,Jinshan Hospital |
Pan C.-F.,Jinshan Hospital |
Wang J.,Jinshan Hospital |
And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012
Objective: To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. Methods: A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. Results: The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.000 compared with normal controls). Conclusions: A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.
Li G.,Fudan University |
Li G.,Jinshan Hospital |
Wei Q.,Fudan University |
Wang Y.,Fudan University |
And 3 more authors.
European Journal of Clinical Microbiology and Infectious Diseases | Year: 2011
The imipenem and meropenem-resistant strains Citrobacter freundii HS70 and Escherichia coli HS510 were isolated from patients in Shanghai, China. By isoelectric focusing, PCR amplification and sequencing, these strains were each found to produce four β-lactamases: TEM-1, KPC-3, SHV-7 and CTX-M-14. A conjugation experiment and plasmid restriction digestion revealed that the blaKPC-3 gene was located on the same plasmid in both isolates. Bidirectional primer walking sequencing showed that the nucleotide sequence surrounding the 3.8 kb blaKPC-3 contained a 671-bp insertion similar to that previously characterized in China. The insertion was located between the promoter and the coding region of the blaKPC-3 gene. Susceptibility testing performed on recombinant strains carrying the blaKPC-3 gene with or without the insertion revealed that minimum inhibitory concentrations of imipenem, meropenem, cefepime, and cefotaxime for E. coli EMU-KPC3 (without insertion) were four times higher than that of E. coli EKPC3 (with insertion). The 671 bp insertion reduced blaKPC-3 expression significantly. Taken together, these results suggest that KPC-3-producing C. freundii and E. coli have begun to emerge in our hospital. © The Author(s) 2010.
Li H.,Jinshan Hospital |
Cheng J.,Fudan University |
Mao Y.,Jinshan Hospital |
Jiang M.,Jinshan Hospital |
And 2 more authors.
OncoTargets and Therapy | Year: 2015
Objective: To investigate the role of miR-21 in cyclooxygenase-2 inhibitor NS398-induced apoptosis and invasion in gastric cancer (GC) cells. Methods: AGS cells were treated with NS398 and transfected with miR-21. Quantitative real-time polymerase chain reaction was used to measure miR-21 mRNA expression. Apoptotic cells were assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling and flow cytometric analysis. The protein expression of cleaved caspase-3, Bcl-2, Bax, Bak, and PTEN was detected by Western blot. The capacities for invasion and migration were measured by transwell and wound-healing assays, respectively. Results: Treatment of AGS cells with NS398 induced apoptosis in a dose-dependent manner accompanied by significant downregulation of miR-21 mRNA expression. Upregulation of miR-21 expression by transfection of miR-21 mimics into AGS cells blocked NS398-induced apoptosis. Treatment of AGS cells with NS398 induced changes in Bcl-2 protein family members, showing an increase in the protein expression of Bax, Bak, and PTEN, with a concomitant decrease in the protein expression of Bcl-2. In cells transfected with miR-21 mimics, these changes were reversed. The decrease in cellular invasiveness and migration induced by NS398 was blocked by upregulation of miR-21. Conclusion: miR-21 mediates anticancer effects of NS398 in GC cells by regulating apoptosis-related proteins. miR-21 is one of the molecular targets of this specific cyclooxygenase-2 inhibitor in the prevention and treatment of GC. © 2015 Li et al.
Ma F.H.,Jinshan Hospital |
Qiang J.W.,Jinshan Hospital |
Cai S.Q.,Jinshan Hospital |
Zhao S.H.,Jinshan Hospital |
And 2 more authors.
American Journal of Roentgenology | Year: 2015
OBJECTIVE. The purpose of this article is to investigate the proton MR spectroscopy (1H-MRS) features of solid adnexal tumors and to evaluate the efficacy of 1H-MRS for differentiating benign from malignant solid adnexal tumors. MATERIALS AND METHODS. Sixty-nine patients with surgically and histologically proven solid adnexal tumors (27 benign and 42 malignant) underwent conventional MRI and 1H-MRS. Single-voxel spectroscopy was performed using the point-resolved spectroscopy localization technique with a voxel size of 2 × 2 × 2 cm3. Resonance peak integrals of choline, N-acetyl aspartate (NAA), creatine, lactate, and lipid were analyzed, and the choline-tocreatine, NAA-to-creatine, lactate-to-creatine, and lipid-to-creatine ratios were recorded and compared between benign and malignant tumors. RESULTS. A choline peak was detected in all 69 cases (100%), NAA peak in 67 cases (97%, 25 benign and 42 malignant), lipid peak in 47 cases (17 benign and 30 malignant), and lactate peak in eight cases (four benign and four malignant). The mean (± SD) choline-tocreatine ratio was 5.13 ± 0.6 in benign tumors versus 8.90 ± 0.5 in malignant solid adnexal tumors, a statistically significant difference (p = 0.000). There were no statistically significant differences between benign and malignant tumors in the NAA-to-creatine and lipid-to-creatine ratios (p = 0.263 and 0.120, respectively). When the choline-to-creatine threshold was 7.46 for differentiating between benign and malignant tumors, the sensitivity, specificity, and accuracy were 94.1%, 97.1%, and 91.2%, respectively. CONCLUSION. Our preliminary study shows that the 1H-MRS patterns of benign and malignant solid adnexal tumors differ. The choline-to-creatine ratio can help clinicians differentiate benign from malignant tumors. © American Roentgen Ray Society.