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Jinshan, China

Chu D.-J.,Jinshan Hospital | Guo S.-G.,Jinshan Hospital | Pan C.-F.,Jinshan Hospital | Wang J.,Jinshan Hospital | And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012

Objective: To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. Methods: A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. Results: The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.000 compared with normal controls). Conclusions: A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease. Source

Li W.,Jinshan Hospital | Wang X.,Fudan University | Chen R.,Affiliated Hospital of Xuzhou Medical University | Zhu H.,Affiliated Hospital of Xuzhou Medical University | And 2 more authors.
Journal of Surgical Research | Year: 2012

Background: Co-stimulatory molecules are pivotal for T cell activation. It is increasingly recognized that programmed death ligand 1 (PD-L1) is a novel co-stimulatory molecule, which raises the question as to whether PD-L1 regulates T cell responses. This study aimed to investigate the inhibitory effects of PD-L1 on T cell activation. Materials and Methods: We constructed a transgenic vector containing the complete PD-L1 gene, which interacts with the inhibitory receptor PD-1 in T cell-mediated immune activation. Donor dendritic cells (DCs) derived from C57BL/6 mice were transfected with PD-L1 and mixed with allogeneic, recipient T cells from BALB/c mice. The T cell activation was determined by the MTT assay and T cell proliferation was determined using carboxyfluoroscein succinimidyl ester (CFSE)-labeling following in vitro mixed leukocyte reactions. Results: The expression of PD-L1 protein in PD-L1-transfected DCs was 47.97% ± 1.06%, compared with 4.66% ± 0.76% and 5.30% ± 0.60% in blank and negative controls, respectively (P < 0.05). PD-L1 protein was effectively expressed in DCs. Furthermore, in DCs stably transfected with PD-L1, T cell activation was significantly suppressed and T cell proliferation rate was decreased by 35% compared with untransfected DCs (P < 0.05). Conclusion: PD-L1 delivers an immunoinhibitory signal, suppressing T cell activation. Overexpression of PD-L1 signaling induces tolerance, which presents a promising immunotherapeutic approach for long-term graft acceptance. © 2012 Elsevier Inc. All rights reserved. Source

Luo Q.,Jinshan Hospital | Luo Q.,Fudan University | Chen Y.,Jinshan Hospital | Chen Y.,Fudan University
Clinical Interventions in Aging | Year: 2016

Long noncoding RNAs (lncRNAs) are typically defined as transcripts longer than 200 nucleotides. lncRNAs can regulate gene expression at epigenetic, transcriptional, and posttranscriptional levels. Recent studies have shown that lncRNAs are involved in many neurological diseases such as epilepsy, neurodegenerative conditions, and genetic disorders. Alzheimer’s disease is a neurodegenerative disease, which accounts for >80% of dementia in elderly subjects. In this review, we will highlight recent studies investigating the role of lncRNAs in Alzheimer’s disease and focus on some specific lncRNAs that may underlie Alzheimer’s disease pathophysiology and therefore could be potential therapeutic targets. © 2016 Luo and Chen. Source

Li H.,Jinshan Hospital | Cheng J.,Fudan University | Mao Y.,Jinshan Hospital | Jiang M.,Jinshan Hospital | And 2 more authors.
OncoTargets and Therapy | Year: 2015

Objective: To investigate the role of miR-21 in cyclooxygenase-2 inhibitor NS398-induced apoptosis and invasion in gastric cancer (GC) cells. Methods: AGS cells were treated with NS398 and transfected with miR-21. Quantitative real-time polymerase chain reaction was used to measure miR-21 mRNA expression. Apoptotic cells were assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling and flow cytometric analysis. The protein expression of cleaved caspase-3, Bcl-2, Bax, Bak, and PTEN was detected by Western blot. The capacities for invasion and migration were measured by transwell and wound-healing assays, respectively. Results: Treatment of AGS cells with NS398 induced apoptosis in a dose-dependent manner accompanied by significant downregulation of miR-21 mRNA expression. Upregulation of miR-21 expression by transfection of miR-21 mimics into AGS cells blocked NS398-induced apoptosis. Treatment of AGS cells with NS398 induced changes in Bcl-2 protein family members, showing an increase in the protein expression of Bax, Bak, and PTEN, with a concomitant decrease in the protein expression of Bcl-2. In cells transfected with miR-21 mimics, these changes were reversed. The decrease in cellular invasiveness and migration induced by NS398 was blocked by upregulation of miR-21. Conclusion: miR-21 mediates anticancer effects of NS398 in GC cells by regulating apoptosis-related proteins. miR-21 is one of the molecular targets of this specific cyclooxygenase-2 inhibitor in the prevention and treatment of GC. © 2015 Li et al. Source

Di Y.,Jinshan Hospital | Di Y.,Fudan University | Lu N.,Jinshan Hospital | Li B.,Jinshan Hospital | And 4 more authors.
Current Eye Research | Year: 2013

Aims: To investigate the effect of prolonged flickering illumination exposure on the growth of the guinea pig eye. Methods: Thirty-six 2-week-old guinea pigs were randomized to one of the three treatment groups (n=12 for each). Two strobe-reared groups were raised with a duty diurnal cycle of 50 % at a flash rate of 0.5Hz and 5Hz respectively. Illumination intensity varied between the minimum-maximum light levels of 0-600lux during each cycle. The control group was exposed to steady 300lux illumination. All animals underwent refraction and biometric measurements prior to and after 2,4,6,8,10 and 12 weeks of treatment. Finally, flash electroretinograms were compared, and retinal microstructures were examined. Results: There was a significant correlation between refractive errors and axial eye elongation, with myopia increasing between 1.5 and 3.4D per mm eye elongation. After 12 weeks of treatment, the animals raised in 0.5Hz flickering light were 5.5±0.4D more myopic than the group raised in continuous illumination, followed by the group raised at 5Hz flicker light which was about 2.2±1.3D more myopic. In animals raised in flickering light of 5 or 0.5Hz for 12 weeks, the implicit time of the a-wave was delayed by 4 and 8.5ms, respectively. At this time, the outer segment disc membranes were found deformed and detached. Conclusion: Chronic exposure to 0.5 and 5Hz temporally modulated illumination induces electrophysiological and histological changes in retinal activities that alter the emmetropization of the guinea pig eye. © 2013 Informa Healthcare USA, Inc. Source

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