PubMed | CAS Shanghai Institutes for Biological Sciences, Liaoning Cancer Hospital and Institute, Jinqiu Hospital of Liaoning Province, Liaoning Medical University and Shenyang Medical College
Type: Journal Article | Journal: Oncology letters | Year: 2017
Lung cancer is a malignant tumor with high morbidity and mortality rates. To date, no suitable molecular diagnostic tool to predict disease recurrence and metastasis has been identified. The current study aimed to evaluate the potential of N-terminal truncated carboxypeptidase E (CPEN) to predict the recurrence and metastasis of lung adenocarcinoma. Western blotting revealed the co-expression of CPE and CPEN in the surgically collected pathological and pericarcinoma tissues tissues of 62.1% (59/95) lung adenocarcinoma patients. The full length CPE protein was predominantly expressed in pericarcinoma tissues and CPEN expression was identified in the pericarcinoma normal tissues of only 5.26% (5/95) patients. The 3-year postoperative recurrence and metastasis rates were significantly higher in patients with positive CPEN expression than in patients with negative CPEN expression (P=0.009). Furthermore, the overall survival rate of patients with predominant nuclear CPE expression was lower than that of patients with predominant cytoplasmic CPE expression (46.3 vs. 64.7%); however, no statistically significant difference was identified (P=0.125). Thus, the results of the current study indicated that CPEN may present a novel molecular biomarker for predicting recurrence and metastasis of lung adenocarcinoma, which may aid with stratifying patients by risk and thus, may facilitate individualized therapy.
Zhang T.-T.,First Peoples Hospital of Yibin |
Liang X.-N.,Jinqiu Hospital of Liaoning Province |
Ren W.,General Hospital of Shenyang Military Command |
Xue W.-C.,General Hospital of Shenyang Military Command |
Yu J.-B.,Liaoning University of Traditional Chinese Medicine
Reviews in Medical Microbiology | Year: 2016
Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS) shows an excellent performance as a tool for microbial identification, which can analyze the strains not only for identification purposes, but also potentially for genetic typing. We present the typing results (compared with multilocus sequence typing) of 290 methicillin-resistant Staphylococcus aureus isolates by MALDI-TOFMS and a review of the literature about the application of MALDI-TOFMS on the molecular typing of other pathogens. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.
Wang K.,Shenyang University |
Cheng L.,Shenyang University |
Liang Y.,Jinqiu Hospital of Liaoning Province |
Liu D.,Jinqiu Hospital of Liaoning Province |
And 2 more authors.
Molecular Biology Reports | Year: 2011
Overexpression of basic fibroblast growth factor (bFGF) has been implicated in the pathogenesis of benign prostatic hyperplasia (BPH) and bFGF has been considered to be a promising therapy target for BPH. RNA interference (RNAi) based therapeutic approaches hold promise for the treatment of a variety of diseases. However, RNAi experiments have seldom been performed in human prostatic stromal cells (PrSCs). In the present study, we tranfected adenovirus type 5 vector mediated small hairpin RNA (shRNA) against human bFGF mRNA (Ad-sh-bFGF) to examine the proliferation and apoptosis effects on cultured human primary PrSCs. The gene-silencing effect of shRNA was evaluated by western blot. Cell proliferation was determined by MTT assays. Cell apoptosis was analyzed by flow cytometry and detection of caspase-3 activity. The effect of Ad-sh-bFGF on Bcl-2 gene expression was also examined. Adenovirus type 5 can efficiently delivered shRNA against bFGF into to PrSCs and the level of protein was depressed significantly in cells infected by Ad-sh-bFGF, approximately 50% lower than those cells infected by adenovirus-delivered nonsense shRNA (P < 0.01). Moreover, Ad-sh-bFGF is able to induce apoptosis and inhibit proliferation of cultured human primary PrSCs significantly (P < 0.01). Bcl-2 protein expression was markedly inhibited by transfection with Ad-sh-bFGF. In conclusion, our findings suggest that RNAi delivered via an adenovirus vector offers a prospect of improvement in treatment of BPH and bFGF is a potential target worth exploiting in BPH. © 2010 Springer Science+Business Media B.V.
Ji Y.,Jinqiu Hospital of Liaoning Province |
Li Y.,Jinqiu Hospital of Liaoning Province |
Lin X.,Shenyang University
Journal of Pure and Applied Microbiology | Year: 2014
As a common infectious disease in the elderly, gingivitis is caused by microbial plaque on tooth supporting tissues. In order to investigate whether there is a linkage between the gingivitis and hemorheology of elderly patients with lacunar infarction, 123 cases of patients were divided into two groups, i.e. a severe gingivitis group (33 patients with a gingival index ≥ 2.1) and a mild gingivitis group (90 patients with a gingival index < 2.1). The hemorheological variables, i.e. whole blood viscosity, plasma viscosity, whole blood reducing viscosity, hematocrit and plasma fibrinogen of these patients were determined. The statistical T-test results showed that the plasma fibrinogen (Fib) (P < 0.01) and whole blood viscosity at middle shear rate (ηbM) (P < 0.05) of patients in the severe gingivitis group were significantly higher than that in the mild gingivitis group. This result revealed that there was a linkage between gingivitis and lacunar infarction. In addition, this study implied that gingivitis would increase the occurrence frequency of lacunar infarction in old people, and the hemorheological variables, ηbM and Fib, might be potential indicators for early diagnostics of lacunar infarction.
Zhang M.,Jinqiu Hospital of Liaoning Province |
Yu L.,Liaoning University of Traditional Chinese Medicine
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Number of experimental and clinical studies have shown that stem cell transplantation can establish new blood vessels and improve heart function instead of necrotic myocardium, to significantly improved clinical symptoms and prognosis of cardiovascular disease patients. Objective: To observe the safety of human umbilical cord blood mononuclear cells transplantation in patients with coronary heart disease and heart failure. Methods: A total of 12 patients with coronary heart disease and heart failure (acute myocardial infarction and heart failure in six cases, old myocardial infarction and heart failure in six cases) were enrolled in this study. Patients were treated on the basis of standard medication and percutaneous coronary intervention. The coronary pathway was established via a percutaneous catheter, and suspension of cord blood mononuclear cells was injected through microcatheter into the distal artery. Routine blood test was carried out at 1 week after cell transplantation, blood routine, liver function, kidney function, C-reactive protein, IgA, IgG were compared preoperatively and postoperatively. Results And Conclusion: The incidence of adverse reactions in cord blood stem cell transplantation was 8.3%, including one case of fever. No micro-embolism occurred. During 1-week follow-up, no graft-versus-host disease appeared. After cell transplantation, there were no significant changes in blood routine, liver function, kidney function, C-reactive protein, IgA, IgG. These findings indicate umbilical cord blood monomuclear cells transplantation is safe in a short term for patients with coronary heart disease and heart failure.
Li X.,Jinqiu Hospital of Liaoning Province |
Shi C.-L.,Jinqiu Hospital of Liaoning Province |
Wang X.-Y.,Jinqiu Hospital of Liaoning Province |
Zhang M.,Jinqiu Hospital of Liaoning Province
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: As the exact mechanism of vascular restenosis after cardiovascular stent implantation is unclear, to reduce the incidence of restenosis after implantation becomes increasingly urgent. The occurrence of vascular restenosis after stent implantation is associated with the corrosion resistance of stents, Young’s modulus, yield strength and material flexibility, as well as the host’s inflammatory response, endothelial cell proliferation, and fibrous tissue proliferation after stent implantation. OBJECTIVE: To analyze the international research trend of cardiovascular stent biocompatibility based on data from Web of Science and Clincaltrials.gov, in order to provide a reference basis for relevant studies. METHODS: (1) Keywords of “cardiovascular stent” and “biocompatibility” were used for article retrieval. (2) The retrieval time was from 2005 to 2014. (3) Information sources: Web of Science and Clincaltrials.gov. RESULTS AND CONCLUSION: (1) Totally 2 875 articles included in Web of Science (2005-2014) were enrolled and analyzed using analytic tools of the Web of Science. Then, eight articles with high citation frequency were obtained. (2) There are 2 875 articles addressing the biocompatibility of cardiovascular stent materials included in the Web of Science (2005-2014), among which, 931 articles come from the U.S.A and account for 32.383%. During 2005-2014, the institutions publishing literatures about biocompatibility of cardiovascular stent materials are ranked as follows: Harvard University, Brigham and Women’s Hospital, Medical University of Vienna, Erasmus University Rotterdam, Columbia University. American Journal of Cardiology has published 117 relevant articles, which ranks the first in this field, and the number of published literature accounts for 4.070% of all literature. In recent years, international literature about cardiovascular stent biocompatibility shows an overall gradual upward trend. Of the 2 875 articles published from 2005 to 2014, 223 articles come from China, ranking the fourth. It indicates that there are more achievements in this field in China. (3) There are 155 clinical trials about the biocompatibility of cardiovascular stent materials included in Clincaltrials.gov from 2005 to 2014, including 140 interventional studies and 15 observational studies. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Zhao W.-B.,Jinqiu Hospital of Liaoning Province |
Li J.,Jinqiu Hospital of Liaoning Province |
Li Y.,Jinqiu Hospital of Liaoning Province |
He Z.-W.,Jinqiu Hospital of Liaoning Province
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Bone cement used in the vertebroplasty treatment of osteoporotic vertebral compression fractures is nonabsorbable and has stronger intensity than normal bone tissue, which can cause further fractures of adjacent segments. OBJECTIVE: To investigate the short-term effect of vertebroplasty with artificial bone in the treatment of osteoporotic thoracolumbar fractures. METHODS: A total of 21 patients with osteoporotic vertebral compression fractures were enrolled, including 7 males and 14 females, aged 61-89 years old. All the patients were subjected to vertebroplasty with calcium sulfate injection. Visual analog scale and Oswestry Disability Index assessment were used before and at 0, 24, 48 weeks after treatment; meanwhile, X-ray and CT were used to observe the filling and leakage of artificial bone. RESULTS AND CONCLUSION: After treatment, all the patients showed improvements in the scores of visual analog scale and Oswestry Disability Index (P < 0.01). X-rays and CT scans showed that there were three cases of leakage but without spinal cord and nerve compressions. After 3 months, most of the artificial bone was absorbed. These findings indicate that vertebroplasty with artificial bone can ease pain and improve living conditions of patients with osteoporotic vertebral compression fractures. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
PubMed | Jinqiu Hospital of Liaoning Province
Type: | Journal: Scientific reports | Year: 2015
Uncertainty exists about the role of diabetes in the development of surgery-related pressure ulcers. Therefore, we conducted a meta-analysis to explore the association between pre-existing diabetes mellitus and pressure ulcers among patients after surgery. Summary odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using random effects models. Thirteen eligible studies of 2367 patients in total and 12,053 controls were included in the final analysis. Compared with patients without diabetes, the pooled odds ratio (OR) of the incidence of pressure ulcers in diabetic patients was 1.74 [95% confidence interval (CI)=1.40-2.15, I(2)=51.1%]. Estimates by type of surgery suggested similar results in cardiac surgery [OR=2.00, 95% CI=1.42-2.82, I(2)=0%], in general surgery [OR=1.75, 95% CI=1.42-2.15, I(2)=0%], and in major lower limb amputations [OR=1.65, 95% CI=1.01-2.68, I(2)=0%] for diabetic patients versus non-diabetic controls. We did not find an increased incidence of pressure ulcers in diabetic patients undergoing hip surgery compared with non-diabetic controls [OR=1.46, 95% CI=0.62-3.47, I(2)=93.1%]. The excess risk of pressure ulcers associated with pre-existing diabetes was significantly higher in patients undergoing surgery, specifically in patients receiving cardiac surgery. Further studies should be conducted to examine these associations in other types of surgery.