Sun H.,Jining First Peoples Hospital |
Yu T.,Jining Medical University |
Li J.,Shanghai Skin Diseases Hospital
Cancer Letters | Year: 2011
Here we report an oral alkylphospholipid perifosine dramatically sensitizes chemo-resistant ovarian cancer cells to paclitaxel induced cell death and apoptosis in vitro. We found that co-administration perifosine with paclitaxel in human ovarian cancer cells led to the inhibition of AKT/mTOR complex 1 (mTORC1), a marked increase in ceramide and reactive oxygen species (ROS) production, and a striking increase in the activation of pro-apoptosis pathways, including caspase 3, c-Jun N-terminal kinases (JNK) and AMP-activated protein kinase (AMPK). These signaling events together caused a marked increase of cancer cell apoptosis. Combining paclitaxel with perifosine may represent a novel anti-ovarian cancer strategy. © 2011 Elsevier Ireland Ltd.
Wang X.-B.,Jining Medical University |
Zhou H.-Y.,Shandong Cancer Hospital and Institute
Biomedicine and Pharmacotherapy | Year: 2015
Molecularly targeted therapy emerged as a novel therapeutic strategy in the treatment of multiple cancers. In the present study, we have developed gemcitabine (GEM)-loaded cetuximab (CET) surface modified poly(lactic) acid (PLA) nanoparticles (NP) (CET-GEM/PLA NP) to target to epidermal growth factor receptor (EGFR) overexpressing non-small cell lung cancer (A549) cells. The resultant CET-GEM/PLA NP showed a very uniform particle size of. ~. 120. nm and spherical morphology. It exhibited a pH-dependent controlled release pattern. A sustained release of drug in the physiological conditions and faster release in tumor pH will greatly improve the chemotherapeutic efficiency of therapeutic system. Higher or enhanced cellular uptake of CET-GEM/PLA NP in A549 cancer cells clearly indicates the EGFR-mediated receptor based active targeting. Nearly, a two-fold increase in fluorescent intensity was observed for CET-GEM/PLA NP comparing to that of non-targeted NP in the cancer cells. EGFR-mediated internalization of the targeted NP was further confirmed by the confocal microscopy. MTT assay clearly showed the enhanced cell killing effect of CET-conjugated NP due to the selective delivery of GEM to the EGFR over expressing cancer cells. Finally, comparing to the non-targeted NP, CET-GEM/PLA NP showed greater level of cell apoptosis (early and late apoptosis. ~. 40%). Our results showed that antibody conjugation on the surface of NP could be a potential treatment strategy for EGFR over expressing cancer cells. This suggests that CET-GEM/PLA NP could be potentially used for the treatment of NSCLC (lung cancers). © 2015 Published by Elsevier Masson SAS.
Qiu C.,Karolinska Institutet |
Qiu C.,Jining Medical University
Journal of Alzheimer's Disease | Year: 2012
Alzheimer's disease (AD) is a major cause of functional dependence, poor quality of life, institutionalization, and mortality among elderly people. As a multifactorial disorder, AD has been frequently linked to vascular risk factors (e.g., smoking, hypertension, obesity, diabetes, hyperlipidemia, and inflammation) in numerous prospective cohort studies of the general population. Systematic reviews and meta-analyses of prospective studies have from the life-course perspective revealed an age-dependent association with the risk of AD for several vascular risk factors such as high blood pressure, obesity, and high total cholesterol, such that possessing these factors in mid-life, but not necessarily in late-life, is associated with an increased risk of AD. The biological plausibility for vascular risk factors to be involved in the pathogenesis and clinical manifestation of Alzheimer syndrome is partly supported by population-based neuroimaging and neuropathological studies. However, randomized controlled trials that target those major cardiovascular risk factors (e.g., antihypertensive, cholesterol-lowering, and anti-inflammatory therapies) have generally failed to prove as efficacious preventative approaches for AD. To bridge the gap, the multifactorial nature of AD and the proper time-window for intervention should be taken into account in the future when designing preventative interventions against this devastating disorder. © 2012 - IOS Press and the authors. All rights reserved.
Yang J.,Jining Medical University |
Wang X.,Heze University |
Shi H.,Qingdao University of Science and Technology
Sensors and Actuators, B: Chemical | Year: 2012
A highly sensitive method for detection of DNA hybridization and phosphinothricin acetyltransferase (PAT) gene fragment, one of the screening detection genes of the transgenic plants, was developed based on the glassy carbon electrode (GCE) modified with polyaniline-(mesoporous nanozirconia)/poly-tyrosine film. The film exhibited excellent properties for immobilizing DNA probes (ss-DNA) and sensing DNA hybridization. The fabrication processes of the film, the DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and found that the electron transfer resistance (Ret) of the electrode surface increased with the concentration of the target DNA hybridized with the DNA probes, which could be applied for determination of the DNA hybridization and PAT gene. The dynamic detection range was from 1.0 × 10 -13 mol L -1 to 1.0 × 10 -6 mol L -1, and the detection limit was 2.68 × 10 -14 mol L -1. Compared with the other electrochemical DNA biosensors based on zirconia-based materials, the proposed biosensor showed its own performance of simplicity, good stability, fine selectivity and high sensitivity. © 2011 Elsevier B.V. All rights reserved.
Ning L.,Jining Medical University
Advanced Materials Research | Year: 2014
The popularity and improvement of resident's electronic health records plays a vital role in the improvement of human's overall health. The basic situation of the construction of residents electronic health records and the implementation of the project progress in a few representative countries all over the world in recent years were been summarized and analyzed in this paper after thorough investigation and research. Some problems existing in the construction and Application process of residents electronic health records were been pointed out, and the development focus of residents electronic health records project in next few years were been pointed out to be the popularize and strengthen of the application of data mining. © (2014) Trans Tech Publications, Switzerland.
Gao H.,Jining Medical University |
Liu C.,Jining Medical University
Biomedicine and Pharmacotherapy | Year: 2014
MicroRNAs are a class of endogenous non-coding RNAs that regulate gene expression at post-transcriptional level, thus participating in diverse biological pathways. Increasing miRNAs are found to dysregulate hepatocellular carcinoma (HCC) and are involved in liver tumorigenesis. In this study, miR-429 was found to obviously downregulate much more in hepatitis B virus (HBV)-related hepatocellular carcinoma. To evaluate the effects of miR-429, miR-429 was over-expressed in HepG2.2.15 cells. The results have proved that overexpression of miR-429 decreased cell proliferation and induced cell apoptosis. Further, overexpression of miR-429 can suppress the secretion of HBsAg and HBeAg. In concordance to this, the level of NOTCH1 expression was high in human HBV-related HCC tissues and HepG2.2.15 cells. MiR-429 directly targeted NOTCH1 and reduced both mRNA and protein levels of NOTCH1 which stimulated proliferation and suppressed apoptosis in HCC cells. Our results provide new insight into the function of miR-429 in HBV-related HCC. It is beneficial to insight into the mechanism of HBV infection and pathophysiology of HBV-related HCC. © 2014 Elsevier Masson SAS.
Yu T.,Jining Medical University
Molecular and cellular biochemistry | Year: 2012
The majority of metastatic melanomas are resistant to different chemotherapeutic agents, consequently, the search for novel anti-melanoma agents and adjuvant is urgent. Here, we found that 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an inhibitor of glycosphingolipid biosynthesis, enhanced curcumin-induced cell growth inhibition and apoptosis in two melanoma cell lines (WM-115 and B16). PDMP facilitated curcumin-induced ceramide accumulation; the latter contributed to melanoma cell apoptosis. PDMP also dramatically enhanced curcumin-induced c-Jun N-terminal kinase activation, which was important to melanoma cell apoptosis. Meanwhile, curcumin plus PDMP treatment largely inhibited the activation of pro-survival PI3K/AKT signal pathway. In conclusion, PDMP-sensitized curcumin-induced melanoma cell growth inhibition and apoptosis in vitro due to changes of multiple signal events. Combining PDMP with curcumin may represent a new therapeutic intervention against melanoma.
Liu C.,Jining Medical University
Archives of Pharmacal Research | Year: 2013
The cancers are a series of serious diseases in the world, and the mechanism involved in many cancers has not yet been fully elucidated. Therefore, it is a very major and significant to explore the molecular mechanisms of cancer occurence and development. MicroRNAs (miRNAs), a class of molecules that regulates gene at post-transcription expression, play an important role in tumorigenesis. It has been proved that a number of miRNAs identified as aberrantly expressed during cancer development. In addition, some of the miRNAs may have prognostic significance. It is the aim of this review to describe the important role of miRNAs in cancer initiation and development as predictive, diagnostic, prognostic biomarkers and novel therapeutic strategies. © 2013 The Pharmaceutical Society of Korea.
Ren Z.-F.,Jining Medical University
International Journal of Modern Physics B | Year: 2011
In He's frequencyamplitude formulation, two trial functions are chosen, which are, respectively, the solutions of linear oscillators. In this paper, an alternative choice of trial function is given: a trial function is a solution of a nonlinear oscillator whose frequency is known. The Duffing equation is used to show the effective of the method. © 2011 World Scientific Publishing Company.
Liu N.,Jining Medical University
Advanced Materials Research | Year: 2014
Residents' health records is different from general hospital medical records, because it is not just about people receive medical service records, or a continuous, sustained, long-term, comprehensive, more extensive information about health information. This article according to the actual situation to the residents of electronic health records system database design are analyzed, and the residents' information table, the doctor information table, a medical information table, health file information table, travel information table and announcement information table 6 design of basic data table made a specific description. © (2014) Trans Tech Publications, Switzerland.