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The majority of metastatic melanomas are resistant to different chemotherapeutic agents, consequently, the search for novel anti-melanoma agents and adjuvant is urgent. Here, we found that 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an inhibitor of glycosphingolipid biosynthesis, enhanced curcumin-induced cell growth inhibition and apoptosis in two melanoma cell lines (WM-115 and B16). PDMP facilitated curcumin-induced ceramide accumulation; the latter contributed to melanoma cell apoptosis. PDMP also dramatically enhanced curcumin-induced c-Jun N-terminal kinase activation, which was important to melanoma cell apoptosis. Meanwhile, curcumin plus PDMP treatment largely inhibited the activation of pro-survival PI3K/AKT signal pathway. In conclusion, PDMP-sensitized curcumin-induced melanoma cell growth inhibition and apoptosis in vitro due to changes of multiple signal events. Combining PDMP with curcumin may represent a new therapeutic intervention against melanoma.

Wang X.-B.,Jining Medical University | Zhou H.-Y.,Shandong Cancer Hospital and Institute
Biomedicine and Pharmacotherapy | Year: 2015

Molecularly targeted therapy emerged as a novel therapeutic strategy in the treatment of multiple cancers. In the present study, we have developed gemcitabine (GEM)-loaded cetuximab (CET) surface modified poly(lactic) acid (PLA) nanoparticles (NP) (CET-GEM/PLA NP) to target to epidermal growth factor receptor (EGFR) overexpressing non-small cell lung cancer (A549) cells. The resultant CET-GEM/PLA NP showed a very uniform particle size of. ~. 120. nm and spherical morphology. It exhibited a pH-dependent controlled release pattern. A sustained release of drug in the physiological conditions and faster release in tumor pH will greatly improve the chemotherapeutic efficiency of therapeutic system. Higher or enhanced cellular uptake of CET-GEM/PLA NP in A549 cancer cells clearly indicates the EGFR-mediated receptor based active targeting. Nearly, a two-fold increase in fluorescent intensity was observed for CET-GEM/PLA NP comparing to that of non-targeted NP in the cancer cells. EGFR-mediated internalization of the targeted NP was further confirmed by the confocal microscopy. MTT assay clearly showed the enhanced cell killing effect of CET-conjugated NP due to the selective delivery of GEM to the EGFR over expressing cancer cells. Finally, comparing to the non-targeted NP, CET-GEM/PLA NP showed greater level of cell apoptosis (early and late apoptosis. ~. 40%). Our results showed that antibody conjugation on the surface of NP could be a potential treatment strategy for EGFR over expressing cancer cells. This suggests that CET-GEM/PLA NP could be potentially used for the treatment of NSCLC (lung cancers). © 2015 Published by Elsevier Masson SAS.

Yang J.,Jining Medical University | Wang X.,Heze University | Shi H.,Qingdao University of Science and Technology
Sensors and Actuators, B: Chemical | Year: 2012

A highly sensitive method for detection of DNA hybridization and phosphinothricin acetyltransferase (PAT) gene fragment, one of the screening detection genes of the transgenic plants, was developed based on the glassy carbon electrode (GCE) modified with polyaniline-(mesoporous nanozirconia)/poly-tyrosine film. The film exhibited excellent properties for immobilizing DNA probes (ss-DNA) and sensing DNA hybridization. The fabrication processes of the film, the DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and found that the electron transfer resistance (Ret) of the electrode surface increased with the concentration of the target DNA hybridized with the DNA probes, which could be applied for determination of the DNA hybridization and PAT gene. The dynamic detection range was from 1.0 × 10 -13 mol L -1 to 1.0 × 10 -6 mol L -1, and the detection limit was 2.68 × 10 -14 mol L -1. Compared with the other electrochemical DNA biosensors based on zirconia-based materials, the proposed biosensor showed its own performance of simplicity, good stability, fine selectivity and high sensitivity. © 2011 Elsevier B.V. All rights reserved.

Liu C.,Jining Medical University
Archives of Pharmacal Research | Year: 2013

The cancers are a series of serious diseases in the world, and the mechanism involved in many cancers has not yet been fully elucidated. Therefore, it is a very major and significant to explore the molecular mechanisms of cancer occurence and development. MicroRNAs (miRNAs), a class of molecules that regulates gene at post-transcription expression, play an important role in tumorigenesis. It has been proved that a number of miRNAs identified as aberrantly expressed during cancer development. In addition, some of the miRNAs may have prognostic significance. It is the aim of this review to describe the important role of miRNAs in cancer initiation and development as predictive, diagnostic, prognostic biomarkers and novel therapeutic strategies. © 2013 The Pharmaceutical Society of Korea.

Su R.,Jining Medical University
Journal of Computational and Theoretical Nanoscience | Year: 2015

With the development of human-computer interface moving in the natural and efficient direction, people pay more and more attentions to the structural analysis and intelligent editing of digital Ink, also known as digital Handwriting. Firstly we puts forward a new method of the multi-level interactional structure analysis and understanding based on context awareness for handwriting, and then we have the intelligent edit operations. The result of experimental evaluation shows that the method has an effective effect for intelligent editing of handwriting. Copyright © 2015 American Scientific Publishers.

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