Luo M.,Jing Gang Shan University |
Luo M.,Wenzhou Medical College |
Zhao A.,Wenzhou Medical College |
Li J.,Southern Medical University |
And 5 more authors.
Immunopharmacology and Immunotoxicology | Year: 2015
Context: Tumor necrosis factor (TNF) α plays a key role in acute liver injury (ALI) induced by injection of d-galactosamine (D-Gal)/lipopolysaccharide (LPS). A novel recombinant trimeric sTNFRII, sTNFRII-gAD, has been tested to be effective in ameliorating ALI, when administered prior to ALI establishment. This study aims to validate the protective effect of sTNFRII-gAD when given after ALI setup and further explore its effect on hepatic apoptosis. Materials and methods: The treatments were carried out concomitantly with ALI establishment with clinically approved sTNFRII-Fc (the dimeric sTNFRII) as a positive control. Lethality, liver weight, and serum alanine transaminase were measured, and histological analysis was performed to evaluate liver injury induced by D-Gal/LPS. Additionally, Terminal-deoxynucleoitidyl transferase-mediated nick end labeling (TUNEL) and Western blot analyses of caspase-3 were used to examine hepatocellular apoptosis. Results: sTNFRII-gAD given after D-Gal/LPS injection turned out to attenuate animal mortality significantly (p<0.01), and had better hepatic protection. In terms of apoptosis, both sTNFRII-gAD and sTNFRII-Fc displayed noticeable improvement of apoptosis evidenced by dramatic decline of active caspase-3 compared to the control group. Conclusions: The results demonstrated that sTNFRII-gAD therapeutically diminished the lethality induced by D-Gal/LPS, possibly through blocking hepatic apoptosis initiated by TNFα. Of note, sTNFRII-gAD was superior to sTNFRII-Fc in some respects, indicating a promising alternative for the therapeutic strategy against the diseases associated with excessive TNFα. © 2015 Informa Healthcare USA, Inc.
He L.,Dalian Maritime University |
Chen Y.,Dalian Maritime University |
Liu L.Y.,Jing Gang Shan University |
Men N.,Dalian Maritime University
Advanced Materials Research | Year: 2013
Through analysis of large sample data of freeway traffic block event, the data source of freeway traffic block is found out and summarized. Based on that, this paper propose a national level, distributed, freeway traffic block multidimensional data warehouse(FTBMDW), and present the concept model, logical model and physical models of the data warehouse. The purpose of the FTBMDW is to support inter-provincial and inter-regional freeway traffic block analysis with reliable data. © (2013) Trans Tech Publications, Switzerland.
He L.,Dalian Maritime University |
Chen Y.,Dalian Maritime University |
Meng N.,Dalian Maritime University |
Liu L.Y.,Jing Gang Shan University
Proceedings - 2011 International Conference of Information Technology, Computer Engineering and Management Sciences, ICM 2011 | Year: 2011
Accurate conceptual model is the foundation of data warehouse building. Studies show that one of the main reasons why a data warehouse project ends up failure is that the modeling of user needs cannot be built up compressively and correctly. Nowadays the way of conceptual modeling has become a central issue in the field of data warehouse research. This article proposes an ontology-based conceptual modeling method for data warehouse, which extending the BWW model according to the special application requirements of data warehouse. The method consists of two parts which are domain modeling and property modeling. Domain modeling describes user needs by a set of theme. Each theme can be depicting by multi-dimension and each dimension can be classified into several classifications. Property modeling, which can solve the heterogeneous problem, is based on BWW ontology, and to represent the identity of dimension, classification, etc, and the relationship between them. This method can formalize the users' needs into a conceptual model with semantic information, which can effectively associate the problem space with the solution space. © 2011 IEEE.
Hu H.-M.,Jing Gang Shan University |
Li W.,Jing Gang Shan University |
Sun X.-M.,Jing Gang Shan University |
Jiang F.,Jing Gang Shan University |
Yang G.-J.,Jing Gang Shan University
Chinese Journal of Tissue Engineering Research | Year: 2014
Background: Spermatogonial stem cells are a kind of adult stem cells, which have self-renewal and differentiation potential, and can be differentiated into specific cells in vitro, suggesting that the spermatogonial stem cells may be possibly differentiated into osteoblasts. But the related research has not been reported. Objective: To observe the biological characterization and osteogenic process of mouse spermatogonial stem cells cultured in vitro. Methods: Spermatogonial stem cells were obtained from the testicle of mice aged 15-20 days, and were cultured on the feeder layer from bone marrow stroma cells in vitro. When cultured for 3 days, the cells were cultured in the conditioned medium (experimental group) and basic medium (control group). The cells proliferation capability and osteogenic property were examined by phase-contrast microscope, alkaline phosphatase activity and type I collagen immunofluorescence staining. Results And Conclusion: Spermatogonial stem cells proliferated faster in the experiment group than in the control group. Cells grew rapidly in colony-like shape in the conditioned medium at 3-6 days, the three-dimensional feeling enhanced, cell mass and clusters continued to increase in size, the extracellular matrix was increased in number and the cytoplasmic bridge was not obvious. After culture for 15 days, cells in the two groups were positive for alkaline phosphatase staining that the cytoplasmic membrane was dyed black. Under the fluorescent microscope, green fluorescence was visible in the experimental group, suggesting the cells in the experimental group was positive for type I collagen, but negative in the control group, which is similar with the biological characteristics of osteoblasts. These findings indicate that spermatogonial stem cells possess the osteogenic capability under induction conditions, which are expected to provide seed cells for bone tissue engineering.
Huang X.-M.,Jing Gang Shan University |
Chem H.-H.,Jing Gang Shan University
Proceedings - 2010 International Conference on Intelligent System Design and Engineering Application, ISDEA 2010 | Year: 2011
According to the load requirements, the DC operating power system adopting high-frequency charger module and maintained-free battery group, system protection methods including TN-S earthing, door body keeping equal potential with the steel track, and also isolating from platform construction are designed for Metro Platform Screen Doors (PSD). One cycle of open and close door is considered a short impact to determine the time of electrical discharge of the battery group. The terminal voltage and battery storage capacity as constraints, the total fee is regarded as a goal function to reasonably obtain the optimized number of high-frequency charger module and battery group by optimized calculation. One optimized example is given. The assessment guidelines on electromagnetic compatibility for the DC Operating Power was proposed, that also could be available for inspection of the same type of subway equipment. The design and assessment guidelines have been widely used in the relevant Platform Screen Door projects. © 2010 IEEE.