Jinan Water and Waste Water Monitoring Center

Jinan, China

Jinan Water and Waste Water Monitoring Center

Jinan, China
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Liu S.Q.,University of Jinan | Chen Y.F.,University of Jinan | Wang L.G.,University of Jinan | Jia R.B.,Jinan Water and Waste Water Monitoring Center
Advanced Materials Research | Year: 2011

It is well known that microcystins (MCs) are the most abundant toxins produced by cyanobacteria in freshwater. The separation and characterization of MCs isomers are very important to the research of algal pollution in freshwater. In this paper MCs isomers were extracted by using methanol water solution and separated by reverse phase high performance liquid chromatography (HPLC). The different isomers were characterized by using HPLC-MS method. Different ratio of extract solvent and chromatographic conditions were discussed. Five MCs isomers were successfully extracted from cyanobacteria of Dianchi Lake. Three of which were characterized to be MC-RR, MC-YR and MC-LR, 1.5mg (92.3 purity) of MC-LR was prepared by using a semi-preparation HPLC system. © (2011) Trans Tech Publications.


Yang H.-Y.,Shandong University | Jia R.-B.,Jinan Water and Waste Water Monitoring Center | Chen B.,Shandong University | Li L.,Shandong University
Environmental Science and Pollution Research | Year: 2014

This study investigates the ability of Rhodococcus sp. strain p52, a dioxin degrader, to biodegrade petroleum hydrocarbons. Strain p52 can use linear alkanes (tetradecane, tetracosane, and dotriacontane), branched alkane (pristane), and aromatic hydrocarbons (naphthalene and phenanthrene) as sole carbon and energy sources. Specifically, the strain removes 85.7 % of tetradecane within 48 h at a degradation rate of 3.8 mg h−1 g−1 dry cells, and 79.4 % of tetracosane, 66.4 % of dotriacontane, and 63.9 % of pristane within 9–11 days at degradation rates of 20.5, 14.7, and 20.3 mg day−1 g−1 dry cells, respectively. Moreover, strain p52 consumes 100 % naphthalene and 55.3 % phenanthrene within 9–11 days at respective degradation rates of 16 and 12.9 mg day−1 g−1 dry cells. Metabolites of the petroleum hydrocarbons by strain p52 were analyzed. Genes encoding alkane-hydroxylating enzymes, including cytochrome P450 (CYP450) enzyme (CYP185) and two alkane-1-monooxygenases, were amplified by polymerase chain reaction. The transcriptional activities of these genes in the presence of petroleum hydrocarbons were detected by reverse transcription-polymerase chain reaction. The results revealed potential of strain p52 to degrade petroleum hydrocarbons. © 2014, Springer-Verlag Berlin Heidelberg.


Chen L.,Shandong University | Wang M.,Shandong University | Jia R.,Jinan Water and Waste Water Monitoring Center | Li L.,Shandong University
Advanced Materials Research | Year: 2012

In this study, the indicators assimilable organic carbon (AOC), dissolved organic carbon (DOC), heterotrophic plate counts (HPC) and microbially available phosphorus (MAP) were measured to evaluate the biological stability of drinking water in Jinan, an east China city, which uses different water sources in its distribution system. The results were shown that the concentration of AOC below 50μg ac-C·L-1 covered the detection of 58% sampling points in the city distribution systems. The difference of source water exercised a great influence to the concentration of AOC. The lower heterotrophic colony counts were detected when the AOC values were higher, and vice versa. The value of AOC/DOC showed its irregularity. MAP was negatively correlated with the AOC. Higher values of AOC and HPC were detected in pumping water than that in pipe water, while values of MAP were equivalent to each other. © (2012) Trans Tech Publications, Switzerland.


Xin X.,Jinan Water and Waste Water Monitoring Center | Wang M.,Jinan Water and Waste Water Monitoring Center | Ge X.,Jinan Water and Waste Water Monitoring Center | Zhao Q.,Jinan Water and Waste Water Monitoring Center | And 2 more authors.
Monatshefte fur Chemie | Year: 2014

Geosmin (GSM) and 2-methylisoborneol (2-MIB) have been identified as the major taste and odor-causing compounds in drinking water obtained from surface water. Carboxylated multi-walled carbon nanotubes (C-MWCNs) were developed for the highly efficient removal of GSM and 2-MIB from water owing to their unique large surface area, well-defined pore size, and high pore volume. Besides, the carboxyl group on the surface of C-MWCNs can improve the adsorption capacity of GSM and 2-MIB which each contain one hydroxyl group. Each adsorption of 200 cm3 1 μg/dm3 of GSM (or 2-MIB) onto 40 mg/dm 3 of C-MWCNs reached equilibrium within 120 min. This agreed well with the Langmuir adsorption model with maximum adsorption capacities of 23.56 and 23.52 μg/g for GSM and 2-MIB. The adsorption rates of GSM and 2-MIB on C-MWCNs fit pseudo-second-order kinetic models (R 2 > 0.99), which suggest that the adsorption process could be a rate-limiting step. Thermodynamic studies illustrated that the adsorption process was endothermic and spontaneous. C-MWCNs are able to remove over 90 % of GSM and 2-MIB in 200 cm3 of solution containing 1 μg/dm3 GSM (or 2-MIB) under optimized conditions. Graphical Abstract: [Figure not available: see fulltext.] © 2014 Springer-Verlag Wien.


Peng P.,Shandong University | Yang H.,Shandong University | Jia R.,Jinan Water and Waste Water Monitoring Center | Li L.,Shandong University
Applied Microbiology and Biotechnology | Year: 2013

A newly isolated Rhodococcus sp. strain p52 could aerobically utilize dibenzofuran as the sole source of carbon and energy, and completely remove dibenzofuran at 500 mg l-1 within 48 h. The strain metabolizes dibenzofuran by initial angular dioxygenation to yield 2,2′,3- trihydroxybiphenyl. Strain p52 could also remove 70 % of 100 mg l-1 2-chlorodibenzofuran within 96 h and could metabolize a variety of aromatic compounds, namely dibenzo-p-dioxin, 2,8-dichlorodibenzofuran, dibenzothiophene, biphenyl, naphthalene, fluorene, phenanthrene, anthracene, carbazole, indole, xanthene, phenoxathiin, xanthone, and 9-fluorenone. Two distinct gene clusters encoding angular dioxygenases (DbfA and DfdA) were amplified and sequenced. The dbfA and dfdA gene clusters are located on two circular plasmids, pDF01 and pDF02, respectively. Both plasmids are self-transmissible; that is, they can transfer to the Gram-positive bacterium Bacillus cereus by conjugation. © 2012 Springer-Verlag.


Liu Y.-M.,Shandong University | Wang M.-H.,Shandong University | Jia R.-B.,Jinan Water and Waste Water Monitoring Center | Li L.,Shandong University
Desalination and Water Treatment | Year: 2012

In the present study, the biocontrol of harmful algae by an algicidal bacterium Aeromonas sp. strain FM was examined. The threshold concentration of FM for its algicidal activity against the exponentially growing Microcystis aeruginosa was 2.1×108 cfumL-1. The influence of temperature on algae removal was tested, and the result indicated that the summer temperature 30°C favored the algicidal activity of the bacterium strain when compared to 20°C, the representative temperature of spring or autumn. A strong algicidal effect of strain FM was observed against M. aeruginosa, M. flos-aquae, Anabaena cylindrica, A. flos-aquae, and Nodularia spumigena. Based on these tests, the removal of M. aeruginosa treated by Aeromonas sp. strain FM harboring in Aquamats was carried out. After 7 d treatment, M. aeruginosa at 7.5×105 cellsmL-1 with a total volume of 10 L decreased 85% of its biomass, following with a decay of the inoculated algicidal strain FM. The electron microscope images demonstrated that the strain severely damaged the cell wall of M. aeruginosa. The results of the present study indicated the application potential of the biocontrol of harmful algae by algicidal bacteria. © 2012 Desalination Publications.


Zhang K.,Shandong Jianzhu University | Du H.,Shandong Jianzhu University | Jia R.,Jinan Water and Waste Water Monitoring Center
Civil Engineering and Urban Planning 2012 - Proceedings of the 2012 International Conference on Civil Engineering and Urban Planning | Year: 2012

The study takes the Yellow River reservoir as water source, and takes the GAC-sand filter as the experimental object. The study compares ordinary sand filter with GAC-sand filter on removal efficiency of turbidity, UV 254, ammonia and MIB. In GAC-sand filter water, turbidity is 0.44NTU, ammonia is 0.19mg/L, UV 254 is 0.018cm -1. The average removal rates of turbidity, ammonia and UV 254 are 47.45 %, 48.65% and 65.62%, respectively. In sand filter water, turbidity is 0.59NTU, ammonia is 0.25mg/L, UV 254 is 0.044cm -1. The average removal rates of turbidity, ammonia, and UV 254 are 29.65%, 35.54%, and 7.77%, respectively. For GAC-sand filter, the removal rate of MIB is up to 89.28%. The experiment shows that GAC-sand filter water quality was significantly better than sand filter water. It is feasible for GAC-sand filter to strengthen technology transformation of sand filter. © 2012 ASCE.


Liu Y.-M.,Shandong University | Chen M.-J.,Shandong University | Wang M.-H.,Shandong University | Jia R.-B.,Jinan Water and Waste Water Monitoring Center | Li L.,Shandong University
Journal of Microbiology and Biotechnology | Year: 2013

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algae-lysis effects of lysine and clavulanate were confirmed. © 2013 by The Korean Society for Microbiology and Biotechnology.


Wang M.-H.,Shandong University | Peng P.,Shandong University | Liu Y.-M.,Shandong University | Jia R.-B.,Jinan Water and Waste Water Monitoring Center | Li L.,Shandong University
Journal of Microbiology and Biotechnology | Year: 2013

Rhodococcus sp. strain p52, a previously isolated dibenzofuran degrader, could effectively inhibit the growth of cyanobacteria, including species of Microcystis, Anabaena, and Nodularia. When strain p52 was inoculated at the concentration of 7.7×107 CFU/ml, 93.5% of exponentially growing Microcystis aeruginosa (7.3×106 cells/ml initially) was inhibited after 4 day. The threshold concentration for its algicidal activity against M. aeruginosa was 7.7×106 CFU/ml. Strain p52 exerted algicidal effect by synthesizing extracellular substances, which were identified as trans-3-indoleacrylic acid, DL-pipecolic acid, and L-pyroglutamic acid. The effective concentrations of trans-3-indoleacrylic acid and DL-pipecolic acid against M. aeruginosa were tested to be 0.5 mg/l and 5 mg/l, respectively. © The Korean Society for Microbiology and Biotechnology.


Liu Y.,Shandong University | Li L.,Shandong University | Jia R.,Jinan Water and Waste Water Monitoring Center
Procedia Environmental Sciences | Year: 2011

Redfield ratio about 16N:1P indicates the atomic ratio of the average elemental composition of phytoplankton, and the initial ratio of resource availability in a system provides the capacity to support algae biomass. Field and laboratory studies, focusing on the natural aquatic environment without disturbance, obtain regular growth of Microcystis aeruginosa with varied Redfield ratios. However, whether there are similar rules of M. aeruginosa under severely disturbing conditions remains unclear. In this study, we reported the growth of M. aeruginosa under conditions with excessive input of nitrogen and phosphorus, which may serve as clues to the control of M. aeruginosa blooming. Growth of M. aeruginosa was monitored in semi-continuous cultures with abundant supply of nitrogen and phosphorus. Effect of nitrogen, phosphorus and their ratio on growth of M. aeruginosa was examined, and the uptake of nitrogen and phosphorus under different nutrient conditions was compared. The effect of N:P ratio depended on the concentration of nitrogen and phosphorus. When initial nitrogen concentration was at 10 mg l-1 and the N:P ratio of 16, the algae growth displayed the highest maximum cell yield and the longest exponential growth. The maximum uptake of nitrogen and phosphorus reached at the N:P ratio of 16, consistent to the maximum yield. On the other hand, when initial phosphorus concentration was around 1 mg l-1, the highest maximum cell yield and the longest exponential growth occurred at the N:P ratio of 40. The ratio of 40 also favored the uptake of phosphorus, while the uptake of nitrogen increased with the increase of nitrogen concentration. © 2011 Published by Elsevier Ltd.

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