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Guangzhou, China

Jinan University is a public research and comprehensive university based in Guangzhou, Guangdong province, China. It is one of the oldest universities established on mainland China tracing back to the Qing Empire. Its mission is to spread Chinese learning and culture from North to South, and from China overseas. Jinan University was the first university in China to recruit foreign students, and is currently the Chinese university with the largest number of international students.In 1996, it was included in the state's Project 211 for development of key universities. It is a university administered by the Overseas Chinese Affairs Office of the State Council and the Chinese Ministry of Education. Wikipedia.

Li Z.,Baylor College of Medicine | Li Z.,Jinan University | Burns A.R.,Baylor College of Medicine | Burns A.R.,University of Houston | And 2 more authors.
FASEB Journal | Year: 2011

After corneal epithelial abrasion, leukocytes and platelets rapidly enter the corneal stroma, and CCR6 + IL-17 + γδ T cells migrate into the epithelium. γδ T-cell-deficient (TCRδ -/-) mice have significantly reduced inflammation and epithelial wound healing. Epithelial CCL20 mRNA increased 19-fold at 3 h, and protein increased ∼16-fold at 6 h after injury. Systemic or topical treatment of wild-type C57BL/6 mice with anti-CCL20 reduced γδ T-cell accumulation in the cornea by >50% with a concomitant decrease in epithelial healing and stromal inflammation. In addition to CCR6 and IL-17, corneal γδ T cells stained positively for RORγt, IL-23R, and IL-22. Anti-IL-22 reduced peak cell division of the healing epithelium by 52%. Treatment of TCRδ -/- mice with rIL-22 significantly promoted wound closure, with peak epithelial cell division increased >3-fold. In addition, rIL-22 restored neutrophil and platelet influx in the TCRδ -/- mice to wild-type levels and increased CXCL1 production by wounded corneal explants >2-fold. These results indicate that an important aspect of the healing response to corneal epithelial abrasion includes CCL20-dependent influx of CCR6 + IL-17 + IL-22 + γδ T cells and that IL-22 contributes to the inflammatory response and promotes epithelial healing. © FASEB.

Fang Z.,South China Normal University | Qiu X.,South China Normal University | Huang R.,South China Normal University | Li M.,Jinan University
Desalination | Year: 2011

Reductive immobilization of chromium in wastewater by nanoscale zero-valent metal (nZVM) prepared from steel pickling waste liquor was investigated. A series of characterization techniques were conducted to characterize the properties of the synthesized nanoparticles. Effects of pH, buffer substances, natural organic matter (NOM) and hardness were evaluated to probe the impact of environmental factors. Removal of Cr(VI) benefited from a decrease in pH value and the presences of buffer substances and Ca2+. NOM was found to be suppressive on the activity of ZVM, and coupled effect of Ca2+ and NOM was additive effect due to their non-interference when operating under acidic condition. The nZVM was proved to be more effective (up to 40.6-fold) than zero-valent iron and nanoscale zero-valent iron for Cr(VI) removal, and its removal capacity in wastewater was 182±2mgg-1. The removal mechanism included mass transfer on solid-liquid interface, reduction of Cr(VI) and simultaneous co-precipitation as Cr-Fe (oxy)hydroxide on the surface of nanoparticles. The results presented herein indicated nZVM was promising for in-situ remediation of Cr(VI). © 2011 Elsevier B.V.

Fan Z.,Jinan University
International Journal of Distributed Sensor Networks | Year: 2013

Duty-cycled operation has been introduced as an efficient way to preserve nodes energy and prolong network lifetime for wireless sensor networks. However, such networks are often logically disconnected since there is a limited number of active nodes within a period of time. Traditional routing algorithms, which have been designed for always-awake wireless networks, suffer excessive waiting time incurred by asynchronous schedule of nodes and cannot be applied to these time-dependent sensor networks. In this work, we study the optimization of delivery delay for low-duty-cycle sensor networks. Specially, we theoretically analyze the sleep latency in low-duty-cycle networks and present a new routing metric, which takes both lossy link and asynchronous schedule of nodes into consideration. Based on the metric, we propose delay-driven routing algorithms to find optimal forwarder in order to reduce delivery delay for source-to-sink communication. We compare our design against state-of-the-art routing algorithms derived in wireless networks through large-scale simulations and testbed experiments, which show that our algorithms can achieve a significant reduction in delivery delay. © 2013 Zuzhi Fan.

Man Z.,Swinburne University of Technology | Wang W.,Defence Science and Technology Organisation, Australia | Khoo S.,Deakin University | Yin J.,Jinan University
Mechanical Systems and Signal Processing | Year: 2012

In this paper, the synchronous signal average of gear mesh vibration signals is modelled with the multiple modulated sinusoidal representations. The signal model parameters are optimised against the measured signal averages by using the batch learning of the least squares technique. With the optimal signal model, all components of a gear mesh vibration signal, including the amplitude modulations, the phase modulations and the impulse vibration component induced by gear tooth cracking, are identified and analysed with insight of the gear tooth crack development and propagation. In particular, the energy distribution of the impulse vibration signal, extracted from the optimal signal model, provides sufficient information for monitoring and diagnosing the evolution of the tooth cracking process, leading to the prognosis of gear tooth cracking. The new methodologies for gear mesh signal modelling and the diagnosis of the gear tooth fault development and propagation are validated with a set of rig test data, which has shown excellent performance. © 2012 Elsevier Ltd.

Zhu X.,Jinan University | Dai S.,Hong Kong University of Science and Technology | Xiang Y.,Hong Kong University of Science and Technology
International Journal of Plasticity | Year: 2013

We present a simulation method for the dynamics of dislocation arrays. In this numerical method, dislocation arrays are considered as continuous surfaces in three dimensions, and the level set representation is used for these dislocation array surfaces. The level set representation of the surfaces has the advantage of automatically handling the topological changes occurring during the evolution, and simple implementation using standard accurate finite difference schemes on a uniform grid. The driving force of the evolution of the dislocation array surfaces comes from both the long-range interaction of the constituent dislocations and their local curvature effect. The long-range interaction, which is expressed by a complicated integral over the whole dislocation array surface, is calculated efficiently using the fast Fourier transform (FFT) method. Simulations are performed for dislocation arrays bypassing different particles under applied stress and are compared with those of a single dislocation. The long-range nature of the stress fields of nonplanar infinite dislocation arrays is discussed, and is shown to be essentially different from that by a single dislocation. Examples also show that such long-range stress fields may contribute to the formation of inhomogeneities of dislocation distributions. © 2012 Elsevier Ltd. All rights reserved.

Tan M.,Jinan University
Neural Processing Letters | Year: 2010

Asymptotic stability problem of a class of fuzzy cellular neural networks with unbounded distributed delays is studied. New stability criteria are derived by employing a Lyapunov-Krasovskii functional and using LMI approach. Numerical examples are provided to illustrate the effectiveness and less conservativeness of the developed techniques. © 2010 Springer Science+Business Media, LLC.

Pan D.,Jinan University
Journal of Convergence Information Technology | Year: 2010

With the growth in the amount of data generated and collected, the need for automatically analyzing the data has received more and more attention from the business community in recent times. It requires some theoretical considerations, which are well understood and popularized throughout the community. The traditional dominant KDD process model offers valuable guidance on the projected-management oriented stages, with no formal foundation to provide guidance in the crucial issue of business understanding, model selection and known knowledge integration. An integrative framework for continuous knowledge discovery process is proposed in evolving environment, and a DIKW (data-information-knowledge-wisdom) conceptual hierarchy to characterize the process operation features and interrelations is discussed. As the theoretical basis, the main notions used in the process model are represented formally, in linear temporal logic.

Akiyama T.,Tokyo Medical University | Shinzawa M.,Tokyo Medical University | Qin J.,Tokyo Medical University | Qin J.,Jinan University | Akiyama N.,Tokyo Medical University
Frontiers in Immunology | Year: 2013

Elimination of potential self-reactive T cells in the thymus is crucial for preventing the onset of autoimmune diseases. Epithelial cell subsets localized in thymic medulla [medullary thymic epithelial cells (mTECs)] contribute to this process by supplying a wide range of self-antigens that are otherwise expressed in a tissue-specific manner (TSAs). Expression of some TSAs in mTECs is controlled by the autoimmune regulator (AIRE) protein, of which dysfunctional mutations are the causative factor of autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). In addition to the elimination of self-reactive T cells, recent studies indicated roles of mTECs in the development of Foxp3-positive regulatory T cells, which suppress autoimmunity and excess immune reactions in peripheral tissues. The TNF family cytokines, RANK ligand, CD40 ligand, and lymphotoxin were found to promote the differentiation of AIRE- and TSA-expressing mTECs. Furthermore, activation of NF-κB is essential for mTEC differentiation. In this mini-review, we focus on molecular mechanisms that regulate induction of AIRE and TSA expression and discuss possible contributions of these mechanisms to prevent the onset of autoimmune diseases. © 2013 Akiyama, Shinzawa, Qin and Akiyama.

The recombinant peptide, DBAYL, a promising therapeutic peptide for type 2 diabetes, is a new, potent, and highly selective agonist for VPAC2 generated through site-directed mutagenesis based on sequence alignments of pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal peptide (VIP), and related analogs. The recombinant DBAYL was used to evaluate its effect and mechanism in blood glucose metabolism and utilization. As much as 28.9 mg recombinant DBAYL peptide with purity over 98% can be obtained from 1 l of Luria-Bertani medium culture by the method established in this study and the prepared DBAYL with four mutations (N10Q, V18L, N29Q, and M added to the N-terminal) were much more stable than BAY55-9837. The half-life of recombinant DBAYL was about 25 folds compared with that of BAY55-9837 in vitro. The bioactivity assay of DBAYL showed that it displaced [(125)I]PACAP38 and [(125)I]VIP from VPAC2 with a half-maximal inhibitory concentration of 48.4 ± 6.9 and 47.1 ± 4.9 nM, respectively, which were significantly lower than that of BAY55-9837, one established VPAC2 agonists. DBAYL enhances the cAMP accumulation in CHO cells expressing human VPAC2 with a half-maximal stimulatory concentration (EC(50)) of 0.68 nM, whereas the receptor potency of DBAYL at human VPAC1 (EC(50) of 737 nM) was only 1/1083 of that at human VPAC2, and DBAYL had no activity toward human PAC1 receptor. Western blot analysis of the key proteins of insulin receptor signaling pathway: insulin receptor substrate 1 (IRS-1) and glucose transporter 4 (GLUT4) indicated that the DBAYL could significantly induce the insulin-stimulated IRS-1 and GLUT4 expression more efficiently than BAY55-9837 and VIP in adipocytes. Compared with BAY55-9837 and PACAP38, the recombinant peptide DBAYL can more efficiently promote insulin release and decrease plasma glucose level in Institute of Cancer Research (ICR) mice. These results suggested that DBAYL could efficiently improve glucose uptake and glucose-dependent insulin secretion by VPAC2-mediated effect.

Wu F.Y.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2010

To investigate the inhibitory effect of survivin antisense oligodeoxynuleotides (ASODN) mediated by polyethylenimine (PEI) on hepatocelluar carcinoma SMMC-7721 cell proliferation and its effect on chemosensitivity to 5-FU in tumor-bearing mice. The inhibitory effect of PEI-ASODN on SMMC-7721 cell proliferation was assayed by WST-8 test, Trypan blue exclusion test, and cell clone formation assay. In mouse models of transplanted H22 cell hepatocarcinoma and ascites tumor, the effect of 5-FU combined with PEI-ASODN on the weight and volume of the subcutaneous tumors was examined. The tumor inhibition rate in the tumor-bearing mice was calculated and the average survival time recorded. SMMC-7721 cells incubated with different concentrations of PEI-ASODN for 48 h showed significantly reduced cell proliferation in comparison with the control cells, while PEI or ASODN alone produced no such inhibitory effect. Incubation of SMMC-7721 cells with 0.75 micromol/L PEI-ASODN for 24, 48, 72, and 96 h resulted in significantly suppressed cell proliferation, and a 7-day incubation of the cells with PEI-ASODN at different concentrations (0.25-0.75 micromol/L) significantly inhibited the cell clone formation. In the tumor-bearing mice, the tumor weight and volume were obviously reduced with a tumor inhibition rate of 56.91% and volume inhibition rate of 57.83%, significantly different from those in saline-treated mice (P<0.01). In the mice bearing ascites tumor, the average survival time was 22.0 days in saline group and 42.7 days in 5-FU+PEI-ASODN treatment group, showing a a life-prolonging rate of 94.09% in the latter group. A synergetic effect was noted between 5-FU and PEI-ASODN. PEI-ASODN complex can significantly inhibit the proliferation of hepatocarcinoma SMMC-7721 cells and enhance 5-FU chemosensitivity of the tumor cells in vitro and transplanted H22 tumors in mice.

Lingyun G.,Jinan University
Acta Mathematica Scientia | Year: 2010

In this article, we mainly investigate the behavior of systems of complex differential equations when we add some condition to the quality of the solutions, and obtain an interesting result, which extends Gackstatter and Laine's result concerning complex differential equations to the systems of algebraic differential equations. © 2010 Wuhan Institute of Physics and Mathematics.

Feng G.C.,Jinan University
Telematics and Informatics | Year: 2015

China's Internet industry has boomed over the last two decades. Given that China is home to the world's largest Internet population, we may wonder what factors have caused this medium, which is strictly controlled in China, to grow and flourish. Based on previous studies and theories, a framework called EPIC (Economy-Policy-Infrastructure-Content) is proposed, and a multivariate time series analysis is performed to examine the relationships between Internet diffusion and these factors. The growth of Internet penetration was found to be mainly driven by Internet content and access cost; however, GDP per capita and telecommunications infrastructure were inconsequential. The implications of these findings are discussed. © 2015 Elsevier Ltd. All rights reserved.

Tan M.,Jinan University
Neural Processing Letters | Year: 2015

In reality, the neural networks (NNs) with different dimensions may be coupled as a complex network. This paper deals with the stabilization problem for an array of coupled NNs with time delays, in which nodes of the isolated NNs have different state dimensions from each other. Without assuming the boundedness or monotonicity of the activation functions and the symmetry or irreducibility of the coupling configuration matrices, several sufficient criteria are derived for ensuring the existence and uniqueness of the equilibrium point of such coupled NNs. The decentralized linear controllers are employed to stabilize the system. By constructing suitable Lyapunov functional, the criteria are formulated in terms of linear matrix inequalities and can be easily verified by using MATLAB LMI Toolbox. Two numerical examples are given to demonstrate the effectiveness of the proposed theoretical results. © 2015 Springer Science+Business Media New York

Tang S.,Jinan University | Rebholz B.J.,Orthopedic Institute of Wisconsin
Journal of Surgical Research | Year: 2013

Background: Microdiscectomy is a common surgical procedure used to treat lumbar disc herniation. Following microdiscectomy, the space of the excised nucleus pulposus is replaced by fibrocartilaginous granulation tissue. This results in alterations of both the material properties of the intervertebral disc and the biomechanics of the lumbar motion segments, potentially contributing to adjacent segmental disc degeneration. To our knowledge, there is no published study in the English literature investigating this potential effect. Materials and methods: A previously developed three-dimensional finite element model of L3-L5 was used as a normal control. From this normal model, two different grades of disc degeneration models (mild and moderate) and corresponding microdiscectomy models were developed by changing either the geometry or associated material properties of L4-L5 segment. The 800 N pre-compressive loading plus 10 Nm moments simulating flexion, extension, lateral bending, and axial rotation were imposed on L3 superior end plate of each model. The intradiscal pressure, intersegmental rotation, and tresca stress of annulus fibrosus in L3-L4 segment were investigated. Results: The intradiscal pressure, intersegmental rotation, and tresca stress of L3-L4 segment in mild degeneration microdiscectomy model are higher than those in the mild degeneration model under all motion directions. The above parameters in moderate degeneration microdiscectomy model present a similar trend to the mild degeneration microdiscectomy model. However, the intersegmental rotation of L3-L4 in moderate degeneration microdiscectomy model is lower than that in the moderate degeneration model in lateral bending, and the intradiscal pressure of L3-L4 in moderate degeneration microdiscectomy model is lower than that in the moderate degeneration model in axial rotation. Conclusions: Lumbar microdiscectomy can result in altered biomechanics, which may have an adverse effect on the development of adjacent upper segmental disc degeneration.

Jiang L.,South China Normal University | Wang Y.,Jinan University | Bjorn L.O.,South China Normal University | Bjorn L.O.,Lund University | Li S.,South China Normal University
Planta | Year: 2011

Even though a number of studies have shown that UV-B radiation inhibits plant growth and regulates the cell cycle progress, little is known about the molecular and cellular mechanisms. Here, we developed a synchronous root-tip cell system to investigate expression changes of cell cycle marker genes and DNA damage under UV-B radiation. Expression analysis of cell cycle marker genes revealed that G1-to-S transition in root-tip cells was accomplished within 6 h. In the in vivo synchronous root-tip cells, high level of UV-B radiation (0.45 W m-2) induced expression changes of the cell cycle regulatory genes. Genes involved in G1-to-S transition, Histone H4 and E2Fa, were down-regulated by UV-B radiation during 2-6 h; whereas transcripts for KRP2, a negative regulator of G1-to-S transition, were up-regulated by UV-B at 2 h. The peak time for transcript level of CYCD3;1, a positive factor in G1-to-S transition, was delayed by UV-B radiation. Interestingly, a medium level of UV-B radiation (0.25 W m-2) did not change the expression of these genes in root tip cells from wild type. However, cell cycle regulatory genes were greatly affected in uvh1 mutant, which exhibited higher content of cyclobutane pyrimidine dimers (CPDs). Ascorbic acid treatment did not change the expression pattern of cell cycle regulatory genes that were affected by high-level UV-B. Our results implied that UV-B-induced DNA damage results in the delay of G1-to-S transition of plant cell cycle. UV-B-induced G1-to-S arrest may be a protective mechanism that prevents cells with damaged DNA from dividing and may explain the plant growth inhibition under increased solar UV-B radiation. © 2011 Springer-Verlag.

Shao Z.-G.,South China Normal University | Ai B.-Q.,South China Normal University | Zhong W.-R.,Jinan University
Applied Physics Letters | Year: 2014

Effect of defects on negative differential thermal resistance (NDTR) is investigated using non-equilibrium molecular dynamics method for the symmetric graphene nanoribbons (GNRs) with defects. Remarkably, it is found that NDTR will disappear as the number of defects increases. In addition, heat current of GNRs with defects is larger than that of the pristine GNRs when the temperature difference is larger. The results are explained qualitatively utilizing the theory of phonon spectra mismatch. The work may be useful in designing thermal-nano devices on the basis of GNRs. © 2014 AIP Publishing LLC.

Xue J.,Jinan University
Thin-Walled Structures | Year: 2012

This paper presents a unique approach to analyze the buckling of an infinitely long cylindrical shell subjected to the external pressure. Buckling is considered to occur locally in the shell, spreading over a certain length along the longitudinal axis of the shell. A plausible function of the flexural displacement is created according to Timonshenkos ring solution of the transverse collapse mode. The governing equations based on DonnellMYVTAPNs shell theory are solved using Ritz method and the equilibrium conditions are educed. Numerical computations are performed for cases when shell thickness/radius ratios are 0.1, 0.05 and 0.03. In general, the pressure decreases sharply with a very slight increase of the normalized radial deflection just at the beginning of the initiation, then falls quite slowly till the two opposite points on the inner surface of the shell contact each other. It is found that the buckling pressure of the shell converges to the critical value given by DonnellMYVTAPNs shell theory and the span of the buckling mode in the longitudinal axis of the shell is independent of material properties. Solutions given in this paper can be used to address the problem of steady-state buckle propagation in the shells. © 2012 Elsevier Ltd. All rights reserved.

Liu S.W.,Jinan University
Sheng li xue bao : [Acta physiologica Sinica] | Year: 2011

The present study aimed to clarify the effect of berberine on the chloride channels in human colorectal carcinoma cells (SW480). The whole-cell patch clamp technique was used to detect the Cl(-) current activated by berberine. The physiological and pharmacological characteristics of the current were clarified by changing the osmotic pressure of extracellular perfusate and applying chloride channel blockers. The results showed that, under isotonic conditions, the background current of SW480 cells was weak and stable. A large current was induced by perfusing the cells with the isotonic solution containing berberine (10 nmol/L), current density being (85.8 ± 4.6) pA/pF at +80 mV, (-71.9 ± 3.5) pA/pF at -80 mV, with a latency of (115.6 ± 21.7) s. The chloride current showed weak outward rectification and negligible time- and voltage-dependent inactivation. The reversal potential (-5.5 mV ± 1.2 mV) of the current was close to the calculated equilibrium potential for Cl(-) (ECl = -0.9 mV). Experiments under different osmotic pressures showed that the properties of hypotonicity-activated current recorded in SW480 cells were similar to those of the current induced by berberine, and hypertonic solutions suppressed the berberine-induced current by (98.6 ± 2.3)%. On the other hand, berberine-induced Cl(-) current was significantly inhibited by the chloride channel blockers NPPB (100 μmol/L) and tamoxifen (20 μmol/L), with the inhibition ratios of (83.1 ± 3.6)% and (95.6 ± 1.2)% respectively. These results suggest that berberine can activate the chloride channels that are sensitive to NPPB and tamoxifen, as well as the changes of cell volume in human colorectal carcinoma cells.

Hu S.,Beijing Normal University | Jin H.,South China Normal University | Chen Z.,Jinan University | Mo L.,South China Normal University | Liu J.,Beijing Normal University
Cortex | Year: 2013

Previous functional magnetic resonance imaging (fMRI) studies have identified several regions in the ventral visual pathway that are specialized for processing faces, words and general objects. However, little is known about the origin of the functional selectivity of these regions. Here, we reported a pediatric patient who suffered a left occipitoparietal lesion in the first year after birth from a subdural hematoma. After the hematoma was removed at the age of six, the hemianopia in the right visual field was alleviated, and no obvious deficits in low-level vision were observed in the patient at the age of twelve. In line with the behavioral observations, meridian mappings with fMRI showed that the early visual cortex of the left hemisphere was significantly activated, which was similar to that of the intact right hemisphere. However, the left ventral temporal cortex failed to show selective responses for faces, words and objects, which were in contrast to the normal selective responses for these objects in the right counterpart. Therefore, it is likely that the development of object selectivity in the ventral temporal cortex depends on visual inputs from the early visual cortex at an early age. © 2013 Elsevier Ltd.

Weilong W.,Sun Yat Sen University | Xiaobo F.,Jinan University
Journal of Nanomaterials | Year: 2013

Fe/Mn mixed metal oxides were synthesized facilely by a grinding method and were characterized by TEM, XRD, XPS, and BET. The characterization results revealed that mixed metal oxides were mainly composed of not highly crystallized Fe2O3 and Mn3O4 nanoparticles with a diameter about 3-5 nm. The specific BET surface areas of the composite were affected by the amounts of KCl diluent in the preparation process and about 268 m2/g of the composite can be achieved. Compared with metal oxide adsorbents existent, the composites showed good adsorption capacity, stability, and regeneration activity for Cr(VI) removal. The enhanced adsorption capacity was speculated to be ascribed to the synergistic effect of the mixed metal oxides. By monitoring the valence change in the adsorption process using XPS characterization, the mechanism for Cr(VI) removal on the composites was found to be a combination of electrostatic attraction and ion exchange. The above results demonstrated that the synthesized metal oxides nanocomposite is of great potential for Cr(VI) removal in the fields of remediation of environmental problems. © 2013 Wang Weilong and Fu Xiaobo.

Zhao T.,Southern Medical University | Li Y.,Southern Medical University | Wei W.,Meizhou Peoples Hospital | Savage S.,Imperial College London | And 2 more authors.
Neurobiology of Disease | Year: 2014

Commonly used anesthetic agents, e.g. ketamine, may be neurotoxic to the developing brain but there has been little attention to the neurobehavioral consequences for offspring when used for maternal anesthesia. We hypothesize that treatment of pregnant rats with ketamine during the second trimester would affect brain development of the offspring. Pregnant rats on gestational day 14, about equal to midtrimester pregnancy in humans, received a sedative dose of ketamine intravenously for 2. h. Brain hippocampal morphology of their pups at postnatal days 0 (P0) and P30 was examined by Nissl-staining and the characteristics of dendrites were determined using the Golgi-Cox staining, while cell proliferation in subventricular zone (SVZ) and dentate gyrus (DG) was labeled with bromodeoxyuridine (BrdU). Their neurobehavioral functions were tested at P25-30 after which the NR1 and NR2 subunits of N-methyl-d-aspartate (NMDA) receptor, brain-derived neurotrophic factor (BDNF) and postsynaptic density protein 95 (PSD-95) in the hippocampus were analyzed by western blot. When pregnant rats were exposed to ketamine, there was neuronal loss, pyramidal neuronal abnormality and reduced cell proliferation in the hippocampus of offspring. These morphological abnormalities were associated with depression- and anxiety-like behaviors, and impaired memory up to young adult age. The treatment further caused NR2A receptor subunit up-regulation and NR2B receptor subunit, BDNF and PSD-95 down-regulation. These data suggest that maternal anesthesia with ketamine during the fetal brain development period can cause fetal brain damage and subsequent neurobehavioral abnormality, which is likely associated with the imbalanced expression of NMDA receptor subunits. © 2014.

Su Y.,Chinese University of Hong Kong | Jiang J.,Chinese University of Hong Kong | Ke N.,Chinese University of Hong Kong | Zhao N.,Chinese University of Hong Kong | And 2 more authors.
Journal of Materials Chemistry C | Year: 2013

Low-voltage, flexibility and low-cost are essential prerequisites for large scale application of organic thin film transistors (OTFTs) in future low-end electronics. Here, we demonstrate a low-voltage flexible OTFT by using a low-temperature, solution-processed gate dielectric. Such a dielectric can be well integrated with an Au coated polyimide film, and exhibits a low leakage current density of less than 10-6 A cm-2 and a high capacitance density of 180 nF cm-2. Pentacene films deposited onto the solution-processed dielectric show a highly ordered "thin film phase". The source-drain (S/D) electrodes are made of in situ modified Cu encapsulated by Au (Au/M-Cu). The obtained flexible OTFT exhibits outstanding electrical characteristics under a gate voltage of only -2 V, which include an on/off ratio of 2 × 104, a mobility (μ) of 1.5 cm 2 V-1 s-1, a threshold voltage (VT) of -0.3 V and a subthreshold slope (SS) of 161 mV dec-1. The obtained mobility value is among the highest achieved in flexible pentacene OTFTs. The mechanical flexibility and reliability of the OTFTs are also studied and discussed in detail, and the observed degradation of the device performance under strains is attributed to the damage induced in the electrodes giving rise to increased contact resistance and the phase transition from the thin film phase to bulk phase of the pentacene films. © The Royal Society of Chemistry 2013.

Dong H.-P.,Jinan University | Dong H.-P.,University of Maryland, Baltimore | Williams E.,University of Maryland, Baltimore | Wang D.-Z.,Xiamen University | And 8 more authors.
Plant Physiology | Year: 2013

The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on Nannochloropsis species have provided insights into the regulation of lipid production in response to nitrogen stress. Previous studies have focused on the responses of Nannochloropsis species to short-term nitrogen stress, but the effect of long-term nitrogen deprivation remains largely unknown. In this study, physiological and proteomic approaches were combined to understand the mechanisms by which Nannochloropsis oceanica IMET1 is able to endure long-term nitrate deprivation and its ability to recover homeostasis when nitrogen is amended. Changes of the proteome during chronic nitrogen starvation espoused the physiological changes observed, and there was a general trend toward recycling nitrogen and storage of lipids. This was evidenced by a global down-regulation of protein expression, a retained expression of proteins involved in glycolysis and the synthesis of fatty acids, as well as an up-regulation of enzymes used in nitrogen scavenging and protein turnover. Also, lipid accumulation and autophagy of plastids may play a key role in maintaining cell vitality. Following the addition of nitrogen, there were proteomic changes and metabolic changes observed within 24 h, which resulted in a return of the culture to steady state within 4 d. These results demonstrate the ability of N. oceanica IMET1 to recover from long periods of nitrate deprivation without apparent detriment to the culture and provide proteomic markers for genetic modification. © 2013 American Society of Plant Biologists. All Rights Reserved.

Ai B.-Q.,South China Normal University | He Y.-F.,Hebei University | Zhong W.-R.,Jinan University
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2010

Directed transport of overdamped Brownian particles driven by fractional Gaussian noises is investigated in asymmetrically periodic potentials. By using Langevin dynamics simulations, we find that rectified currents occur in the absence of any external driving forces. Unlike white Gaussian noises, fractional Gaussian noises can break thermodynamical equilibrium and induce directed transport. Remarkably, the average velocity for persistent fractional noise is opposite to that for antipersistent fractional noise. The velocity increases monotonically with Hurst exponent for the persistent case, whereas there exists an optimal value of Hurst exponent at which the velocity takes its maximal value for the antipersistent case. © 2010 The American Physical Society.

Zhong W.-R.,Jinan University
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2010

A double-stranded system, modeled by a Frenkel-Kontorova lattice, is studied through nonequilibrium molecular dynamics simulations. We have investigated the thermal conductance influenced by the intrachain interaction as well as by the interchain interaction. It is found that the intrachain interaction always enhance the thermal conductance. The interchain interaction, however, has a positive effect on the thermal conductance in the case of strong nonlinear potential, and has a negative effect on the thermal conductance in the case of weak nonlinear potential. This phenomenon can be explained by the transition of thermal transport mode and the phonon band shift of the particles. It is suggested that the inter- and intrachain interactions present different thermal properties in double-stranded lattices. © 2010 The American Physical Society.

Tang S.,Jinan University | Meng X.,Central Hospital of Yidu
Turkish Neurosurgery | Year: 2011

Aim: The restoration of disc space height of fused segment is essential in anterior lumbar interbody fusion, while the disc space height in many cases decreased postoperatively, which may adversely aggravate the adjacent segmental degeneration. However, no literature available focused on the issue. Material and Methods: A normal healthy finite element model of L3-5 and four anterior lumbar interbody fusion models with different disc space height of fused segment were developed. 800 N compressive loading plus 10 Nm moments simulating exion, extension, lateral bending and axial rotation were imposed on L3 superior endplate. The intradiscal pressure, the intersegmental rotation, the tresca stress and contact force of facet joints in L3-4 were investigated. Results: Anterior lumbar interbody fusion with severely decreased disc space height presented with the highest values of the four parameters, and the normal healthy model presented with the lowest values except, under extension, the contact force of facet joints in normal healthy model is higher than that in normal anterior lumbar interbody fusion model. With disc space height decrease, the values of parameters in each anterior lumbar interbody fusion model increase gradually. Conclusion: Anterior lumbar interbody fusion with decreased disc space height aggravate the adjacent segmental degeneration more adversely.

Ge R.,Sun Yat Sen University | Sun X.,Jinan University | Wang D.,Sun Yat Sen University | Zhou Q.,Sun Yat Sen University | Sun H.,University of Hong Kong
Biochimica et Biophysica Acta - Molecular Cell Research | Year: 2011

Helicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. © 2011 Elsevier B.V.

Peng G.,Jinan University
Physica A: Statistical Mechanics and its Applications | Year: 2010

We study the validity of Zipf's Law in a data set of Chinese city sizes for the years 19992004, when the numbers of cities remain almost constant after a rapid urbanization process during the period of the market-oriented economy and reform-open policy. Previous investigations are restricted to loglog ranksize regression for a fixed sample. In contrast, we use rolling sample regression methods in which the sample is changing with the truncation point. The intuition is that if the distribution is Pareto with a coefficient one (Zipf's law holds), rolling sample regressions should yield a constant coefficient regardless of what the sample is. We find that the Pareto exponent is almost monotonically decreasing in the truncation point; the mean estimated coefficient is 0.84 for the full dataset, which is not so far from 1. © 2010 Elsevier B.V.

Liu Q.,Baylor College of Medicine | Smith C.W.,Baylor College of Medicine | Zhang W.,University of Houston | Burns A.R.,Baylor College of Medicine | And 3 more authors.
American Journal of Pathology | Year: 2012

Natural killer (NK) cells are lymphocytes of the innate immune system that have crucial cytotoxic and regulatory roles in adaptive immunity and inflammation. Herein, we consider a role for these cells in corneal wound healing. After a 2-mm central epithelial abrasion of the mouse cornea, a subset of classic NK cells migrated into the limbus and corneal stroma, peaking at 24 hours with an eightfold increase over baseline. Depletion of γδ T cells significantly reduced NK cell accumulation (>70%; P < 0.01); however, in neutrophil-depleted animals, NK cell influx was normal. Isolated spleen NK cells migrated to the wounded cornea, and this migration was reduced by greater than 60% (P < 0.01) by ex vivo antibody blocking of NK cell CXCR3 or CCR2. Antibody-induced depletion of NK cells significantly altered the inflammatory reaction to corneal wounding, as evidenced by a 114% increase (P < 0.01) in neutrophil influx at a time when acute inflammation is normally waning. Functional blocking of NKG2D, an activating receptor for NK cell cytotoxicity and cytokine secretion, did not inhibit NK cell immigration, but significantly increased neutrophil influx. Consistent with excessive neutrophil accumulation, NK depletion and blocking of NKG2D also inhibited corneal nerve regeneration and epithelial healing (P < 0.01). Findings of this study suggest that NK cells are actively involved in corneal healing by limiting the innate acute inflammatory reaction to corneal wounding. © 2012 American Society for Investigative Pathology.

Liu J.,University of Saskatchewan | Shim Y.Y.,University of Saskatchewan | Poth A.G.,University of Queensland | Reaney M.J.T.,University of Saskatchewan | Reaney M.J.T.,Jinan University
Food Hydrocolloids | Year: 2016

Flaxseed gum (FG) is a mixture of natural polysaccharide and protein derived from flaxseed (Linum usitatissimum L.) that has potential for thickening foods, stabilizing emulsions, and gelling solutions. The composition and identities of proteins in FG have never been reported. In this study, gum prepared from whole flaxseed was deglycosylated by treating it with trifluoromethanesulfonic acid (TFMS). The resultant proteins were separated by 2D-gel electrophoresis. The major protein spots with estimated molecular weights (MW) of 10-11 kDa and 11-12 kDa were excised, digested with trypsin, and analyzed using matrix-assisted laser ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Peptide MS of tryptic digestion fragments was compared to MS of peptides from gene models available through National Center for Biotechnology Information (NCBI) database. Fragments consistent with the seed storage protein conlinin, the low-molecular-mass 2S storage flaxseed protein, were identified as the major spot constituents. Emulsification properties of FG were determined before and after protease hydrolysis with emulsion activity index (EAI) and emulsion stability (ES) as indicators. Both EAI and ES decreased from 98.7 ± 5.4 to 59.9 ± 3.2 m2/g and from 66.4 ± 1.1 to 42.1 ± 2.0%, respectively, after protease treatment. Conlinin is the major protein associated with FG and it plays a fundamental role in determining FG emulsification properties. © 2015 Elsevier Ltd.

The paper took Tencent enterprise microblog platform as example, using experiment to discover what kind of microblog text can effectively influence users' attitudes and behaviors. It was found that advertising information can not significantly changed users' attitudes toward the brand, but non-advertising information can significantly change users' attitudes toward the company's microblog, and ultimately change their attitudes toward brand. Some suggestions for business marketing on microblog were also proposed. © (2014) Trans Tech Publications, Switzerland.

Ai B.-Q.,South China Normal University | Zhong W.-R.,Jinan University | Hu B.,University of Houston
Journal of Physical Chemistry C | Year: 2012

Negative differential thermal resistance (NDTR) in approximate graphene nanoribbons (GNRs) is investigated from one dimension to three dimensions by using classical molecular dynamics method. For single-layer GNRs, NDTR cannot be observed for very narrow GNRs (one dimension), and NDTR appears when the width of GNRs increases (two dimensions). However, NDTR disappears gradually on further increasing the width. For multiple-layer GNRs, when the number of the layers increases, GNRs change from a 2-D system to 3-D system, and NDTR regime reduces and eventually disappears. In addition, when the length of GNRs increases, NDTR regime also reduces and vanishes in the thermodynamic limit. These effects may be useful for designing thermal devices where NDTR plays an important role. © 2012 American Chemical Society.

Feng H.,Jinan University
2011 2nd International Conference on Artificial Intelligence, Management Science and Electronic Commerce, AIMSEC 2011 - Proceedings | Year: 2011

Notice of Retraction After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE's Publication Principles We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper. The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.

Pleissner D.,City University of Hong Kong | Lau K.Y.,City University of Hong Kong | Zhang C.,Jinan University | Lin C.S.K.,City University of Hong Kong
ChemSusChem | Year: 2015

The potential of lipids derived from food-waste and algal biomass (produced from food-waste hydrolysate) for the formation of plasticizers and surfactants is investigated herein. Plasticizers were formed by epoxidation of double bonds of methylated unsaturated fatty acids with in situ generated peroxoformic acid. Assuming that all unsaturated fatty acids are convertible, 0.35 and 0.40 g of plasticizer can be obtained from 1 g of crude algae- or food-waste-derived lipids, respectively. Surfactants were formed by transesterification of saturated and epoxidized fatty acid methyl esters (FAMEs) with polyglycerol. The addition of polyglycerol would result in a complete conversion of saturated and epoxidized FAMEs to fatty acid polyglycerol esters. This study successfully demonstrates the conversion of food-waste into value-added chemicals using simple and conventional chemical reactions. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Li K.,University of Connecticut Health Center | Zhang J.,Jinan University | Li J.Y.H.,University of Connecticut Health Center
PLoS ONE | Year: 2012

Unlike the laminar arrangement of neurons in the neocortex, thalamic neurons aggregate to form about dozens of nuclei, many of which make topographic connections with specific areas in the neocortex. The molecular mechanisms underlying the formation of thalamic nuclei remain largely unknown. Homeodomain transcription factor Gbx2 is specifically expressed in the developing thalamus. Deleting Gbx2 leads to severe disruption of the histogenesis of the thalamus in mice, demonstrating an essential role of Gbx2 in this brain structure. Using inducible genetic fate mapping, we have previously shown that the neuronal precursors for different sets of thalamic nuclei have distinctive onset and duration of Gbx2 expression, suggesting that the dynamic expression of Gbx2 plays an important role in the specification and differentiation of thalamic nuclei. Here, we showed that the Gbx2 lineage exclusively gives rise to neurons but not glia in the thalamus. We performed conditional deletion to examine the temporal requirements of Gbx2 in the developing thalamus in mice. Corresponding to the dynamic and differential expression of Gbx2 in various thalamic nucleus groups, deleting Gbx2 at different embryonic stages disrupts formation of distinct sets of thalamic nuclei. Interestingly, different thalamic nuclei have remarkably different requirements of Gbx2 for the survival of thalamic neurons. Furthermore, although Gbx2 expression persists in many thalamic nuclei until adulthood, only the initial expression of Gbx2 following neurogenesis is crucial for the differentiation of thalamic nuclei. Our results indicate that the dynamic expression of Gbx2 may act as an important determinant in coupling with other developmental programs to generate distinct thalamic nuclei. © 2012 Li et al.

Wang H.,Rutgers University | Yao K.,King Abdullah University of Science and Technology | Zhang Z.,Jinan University | Jagiello J.,Micromeritics Instrument Co. | And 3 more authors.
Chemical Science | Year: 2014

In industry, cryogenic rectification for separating xenon from other noble gases such as krypton and argon is an energy and capital intensive process. Here we show that a microporous metal-organic framework, namely Co 3(HCOO)6 is capable of effective capture and separation of xenon from other noble gases. Henry's constant, isosteric heat of adsorption (Qst), and IAST selectivity are calculated based on single component sorption isotherms. Having the highest Qst reported to date, Co 3(HCOO)6 demonstrates high adsorption capacity for xenon and its IAST selectivity for Xe-Kr is the largest among all MOFs investigated to date. To mimic real world conditions, breakthrough experiments are conducted on Xe-Kr binary mixtures at room temperature and 1 atmosphere. The results are consistent with the calculated data. These findings show that Co 3(HCOO)6 is a promising candidate for xenon capture and purification. Our gas adsorption measurements and molecular simulation study also reveal that the adsorption of xenon represents the first example of commensurate adsorption of atomic gases near ambient conditions. © 2014 The Royal Society of Chemistry.

Yang Q.H.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

To research the effects of soothing liver and invigorating spleen recipes on expression of TLR4 mRNA and protein expression in hepatic tissue of rats with non-alcoholic steatohepatitis and its mechanism. 72 male SD rats were randomly divided into 8 groups: normal group, model group, high-dose soothing liver group (receiving gavage of Chaihu Shugan Powder 9. 6 g/kg), low-dose soothing liver group (receiving gavage of Chaihu Shugan Powder 3.2 g/kg), high-dose invigorating spleen group (receiving gavage of Shen Ling Baizhu Powder 30 g/kg), low-dose invigorating spleen group (receiving gavage of Shen Ling Baizhu Powder 10 g/kg), high-dose integrated Group (receiving gavage of Chaihu Shugan Powder and Shen Ling Baizhu Powder combination recipes 39.6 g/kg), low-dose integrated Group (receiving gavage of Chaihu Shugan Powder and Shen Ling Baizhu Powder combination recipes 13.2 g/kg), 9 rats were in each group. Used high fat diet (10 mL/kg) to establish experiment model of NASH rat. At the end of the sixteenth weeks, the levels of serum lipids, liver lipids and serum aminotransferase were measured by automatic biochemical analyzer; Liver pathology was analyzed by HE and Oil red O staining; TLR4 mRNA was assayed by real-time fluorescent quantitative polymerase chain reaction (RT Q-PCR); TLR4 protein was detected by Western blot. Compared with normal group, the levels of TC, LDL-C in the serum, TC,TG as well as the expression of TLR4 mRNA and protein in the hepatic tissue were dramatically increased in model group (P < 0.01). Compared with the model group, the levels of serum lipids, liver lipids, the expression of TLR4 mRNA and protein in the hepatic tissue were decreased in each treatment group (P < 0.05 or P < 0.01). Soothing liver and invigorating spleen recipes can inhibit hepatic TLR4 expression, that may be one of their therapeutic mechanisms. There is much difference between high-does and low-does treatment groups in various testing items, which shows that there is does-effect relationship in intervention NASH.

Huang F.,Jinan University
Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji | Year: 2011

Ferulic acid (FA) is a natural compound that expresses antioxidant and anti-inflammatory activities. Microglial cells are innate immune cells that reside within the central nervous system (CNS). Activated microglia mediated neuronal immunity contributes to the neurodegeneration associated with Alzheimer's disease. In this study, we investigated the inhibitory effect of FA on neuroinflammation in BV-2 microglial cells induced by lipopolysaccharides (LPS). Our study showed that FA significantly suppressed the production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1β (IL-1β), and decreased induced type II nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein in LPS-stimulated BV-2 microglia cells in a dose dependent manner. We hypothesized that this was achieved by suppressing the protein level of Toll-like receptor 4 (TLR4).

Han X.-H.,Guangdong University of Technology | Xue S.-J.,Jinan University
Jisuanji Jicheng Zhizao Xitong/Computer Integrated Manufacturing Systems, CIMS | Year: 2010

In order to increase operational efficiency, the reverse channel decision was studied based on evolutionary game in the closed-loop supply chain with competitive manufactures and one dominant retailer. Results indicated that the competition among manufacturers, the collection difficulty and remanufacturing cost saving influenced the evolution result of reverse channel together. And by adopting subsidy policy, government encouraged manufacturers to choose direct reverse channel, which was more benefit to customers and society.

Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
Journal of the Mechanics and Physics of Solids | Year: 2014

We present a continuum framework for dislocation structure, energy and dynamics of dislocation arrays and low angle grain boundaries that are allowed to be nonplanar or nonequilibrium. In our continuum framework, we define a dislocation density potential function on the dislocation array surface or grain boundary to describe the orientation dependent continuous distribution of dislocations in a very simple and accurate way. The continuum formulations incorporate both the long-range dislocation interaction and the local dislocation line energy, and are derived from the discrete dislocation model. The continuum framework recovers the classical Read-Shockley energy formula when the long-range elastic fields of the low angle grain boundaries are canceled out. Applications of our continuum framework in this paper are focused on dislocation structures on static planar and nonplanar low angle grain boundaries and misfitting interfaces. We present two methods under our continuum framework for this purpose, including the method based on the Frank×3.

Niu B.,Shenzhen University | Niu B.,Chinese Academy of science | Niu B.,China Institute of Technology | Wang H.,Shenzhen University | And 2 more authors.
Neurocomputing | Year: 2013

This paper describes a novel Bacterial Foraging Optimization (BFO) approach to multi-objective optimization, called Multi-objective Bacterial Foraging Optimization (MBFO). The objectives in the Multi-objective Bacterial Foraging Optimization are maintained by a fitness survive mechanism. Bacteria with the smaller health values have the better chance to survive. Meanwhile, the main goal of multi-objective optimization problems is to obtain a superior non-dominated front which is closed to the true Pareto front. With identification of such features, the idea of integration between health sorting approach and pareto dominance mechanism are developed to search for Pareto-optimal set of problems. Moreover, strategy keeping a certain unfeasible border solutions based on a given probability is considered to improve the diversity of individuals. In addition, two different performance metrics: Diversity and Generational Distance are introduced as well to evaluate multi-objective optimization problems. Compared to two other multi-objective optimization evolutionary algorithms MOPSO and NSGA-II, simulation results show that in most cases, the proposed MBFO is able to find a much better spread of solutions and convergence to the true Pareto-optimal front faster. It suggests that MBFO is very promising in dealing with ordinary multi-objective optimization problems. © 2012 Elsevier B.V.

Yang L.,Jinan University
Wireless Personal Communications | Year: 2010

In this paper, we present a comprehensive performance analysis ofmultiple-input multiple-output (MIMO) systems with transmit antenna selection (TAS) and stochastic power allocation (SPA) for the spatially correlated fading channels. Two best transmit antennas that maximize the instantaneous received signal-to-noise (SNR) are selected to transmit the Alamouti scheme and maximal-ratio combining (MRC) is applied at the receiver. With correlation matrices available to the transmitter, SPA is applied on these selected antennas. Two different methods are given to derive the explicit upper bounds on the bit-error rate (BER) performance. Finally we present numerical results to verify our analysis. It is shown that the TAS/SPA scheme can achieve high performance in spatially correlated channels. © Springer Science+Business Media, LLC. 2009.

Peng H.,Jinan University
Lecture Notes in Electrical Engineering | Year: 2013

The purpose of the paper is to research the constraint factors of group purchasing tourism products online in the new electronic marketing channel. The scale of constraint factors of group purchasing online was developed, and the questionnaire method was used in the study. The result shows that the constraint factors of group purchasing tourism products online include four dimensions, and the website information and perceived risk are the main obstacle factors for the tourism product's group purchasing online. There are different impacts on the constraint factors for the partial demographic and group purchasing habit factors. The website of group purchasing tourism products should take the corresponding measures according to the constraint factors to improve the consumers' intention of tourism product group purchasing online. © 2013 Springer-Verlag.

Tang S.H.,Jinan University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To evaluate the efficacy and safety of the angiotensin II receptor blockers (ARB) in reducing portal hypertension (PHT) in patients with cirrhosis. PubMed, EMBASE, Web of Science, The Cochrane Central Register of Controlled Trials, Chinese Biomedical Database, Chinese Journals Full-text Database and WanFang Digital Journal Full-text database were searched. Statistical analysis was performed by meta-analysis using RevMan4.2 software. Among 8 randomized controlled trials (RCT) including 282 patients met the inclusion criteria, 4 trials were analyzed to compare the ARB with the placebo or no treatment and the other 4 trials were analyzed to compare the ARB with propranolol. Meta-analysis results were as follows. (1)The ARB resulted in more significant hepatic venous pressure gradient (HVPG) reduction as compared with the placebo or no treatment [WMD = 1.87 mm Hg (1 mm Hg = 0.133 kPa), 95%CI (0.86 - 2.87) mm Hg, P = 0.00003]. And the ARB were similar to propranolol in reducing HVPG [WMD = 0.92 mm Hg, 95%CI (-0.41 - 2.26) mm Hg, P = 0.17]. (2)The ARB led to more significant reduction in mean arterial pressure than the placebo or no treatment [WMD = 8.89 mm Hg, 95%CI (7.16 - 10.62) mm Hg, P < 0.000 01], but they were similar to propranolol in giving rise to mean arterial pressure reduction (WMD = 0.41, 95%CI -4.46 - 5.28, P = 0.87) which had no significant difference. And the ARB had no significant effect on the heart rate of the patients, which was similar to no treatment group (P > 0.05). Whereas, propranolol could greatly decrease heart rate of the patients (WMD = -21.25, 95%CI -25.83 - 16.68, P < 0.000 01). (3) No significant differences were found in serum bilirubin and creatinine levels between the ARB and the placebo or no treatment groups (P > 0.05). The rate of nonspecific adverse events was higher in the ARB groups than in the placebo or no treatment groups (P = 0.03), but it showed there was no difference between the ARB and propranolol groups (P = 0.72). The ARB is effective in reducing portal hypertension in patients with cirrhosis, which is similar to propranolol. Their effects on mean arterial pressure is similar to propranolol without significant effects on hear rate, liver function and kidney function, and with less nonspecific adverse events. The ARB could become a new choice for the treatment of portal hypertension.

He G.-N.,Sun Yat Sen University | Huang B.,Jinan University | Shen H.,Sun Yat Sen University
Journal of Crystal Growth | Year: 2010

Vertically aligned arrays of ZnO nanorod were synthesized on the Au/SiO2/Si(1 0 0) substrate by a simple aqueous solution growth process, without pre-prepared ZnO seed layer. For comparison, glass and SiO 2/Si were also used as substrates, and the results show that the Au layer plays a decisive role in orienting the growth of the ZnO nanorod. The effects of other growth parameters, including Zn2 concentration and growth time, on morphology, density, and orientation of the ZnO nanostructure were also studied and with longer reaction time, a new structure namely ZnO nanotip was obtained. Moreover, the growth mechanism of ZnO nanorod arrays grown on the Au/SiO2/Si substrate was proposed. © 2010 Elsevier B.V. All rights reserved.

Zhu X.,Hong Kong University of Science and Technology | Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
Scripta Materialia | Year: 2011

In this paper, we study the glide force due to stress on the constituent dislocations of slightly perturbed symmetric low-angle tilt boundaries. We show that the stabilizing force comes from both the long-range interaction of the constituent dislocations and their local line tension effect. We also present a continuum model for this glide force. The obtained results and continuum model provide a basis for further understanding of the stress-driven migration of distorted grain boundaries. © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Zhou M.,Jinan University | Wong M.H.,Chinese University of Hong Kong
Information Sciences | Year: 2011

Dynamic time warping (DTW) is a powerful technique in the time-series similarity search. However, its performance on large-scale data is unsatisfactory because of its high computational cost and the fact that it cannot be indexed directly. The lower bound technique for DTW is an effective solution to this problem. In this paper, we explain the existing lower-bound functions from a unified perspective and show that they are only special cases under our framework. We then propose a group of lower-bound functions for DTW and compare their performances through extensive experiments. The experimental results show that the new methods are better than the existing ones in most cases, and a theoretical explanation of the results is also given. We further implement an index structure based on the new lower-bound function. Experimental results demonstrate a similar conclusion. © 2011 Elsevier Inc. All rights reserved.

Gong Q.,Nanyang Normal University | Qin Y.,Jinan University
Zhongguo Jiguang/Chinese Journal of Lasers | Year: 2010

An approach based on the correlation between two intensity distribution images of the object wave is proposed to determine the phase-shifting value in two-step phase-shifting digital holography. By changing the phase-shifting values used for reconstruction in a certain range around the nominal phase-shifting value, a series of correlation coefficients between the object intensity recorded by CCD and the same intensity reconstructed by computer are obtained. The accurate phase-shifting value will be determined when the correlation coefficient reaches its maximum. The real phase-shifting value is successfully extracted in computer simulations by this method, thereafter the approach is employed in LED-based digital holography. The quality of the reconstructed field by using the obtained value and the nominal value are compared. The feasibility of this method has been verified by both computer simulations and experiments.

Aim: To determine whether transforaminal lumbar interbody fusion aggravate adjacent segmental degeneration more adversely than anterior lumbar interbody fusion. Material and Methods: A normal finite element model, an anterior lumbar interbody fusion model and a transforaminal lumbar interbody fusion model of L3-5 were developed. 800 N compressive loading plus 10 Nm moments simulating flexion, extension, lateral bending and axial rotation were imposed on L3 superior endplate. The intradiscal pressure, intersegmental rotation and tresca stress in L3-4 were investigated. Results: Under all loading directions, the values of three parameters in transforaminal lumbar interbody fusion model were the highest and those in normal model were the lowest, and the values in the ALIF model were higher than the normal model while lower than the TLIF model. In the ALIF and normal models, the values of the three parameters on left lateral bending and axial rotation were equal to those on right lateral bending and axial rotation. However, in the TILF model, the values on right lateral bending and axial rotation were larger than those on left lateral bending and axial rotation. Conclusion: Transforaminal lumbar interbody fusion aggravates adjacent segmental degeneration more adversely than anterior lumbar interbody fusion.

Chen J.-F.,Jinan University | Morozov E.V.,University of New South Wales
Composite Structures | Year: 2016

This paper is concerned with the development and validation of a consistency elasto-viscoplastic damage model that accounts for the strain rate-dependent plastic response and the progressive post-failure behaviour of composite materials. The model proposed is suitable for progressive failure analysis of composite materials and structures subjected to loadings at various strain rates. A strain rate-dependent yield criterion is adopted; whereas the standard Kuhn-Tucker conditions for plastic loading and unloading remain valid. Also, the plastic consistency condition for strain rate-dependent material is satisfied. The relevant strain-driven implicit numerical integration procedure for the proposed material model is developed. A tangent stiffness operator including the strain rate-dependent effects and consistent with the integration procedure developed is derived to ensure the quadratic convergence rate pertaining to the Newton-Raphson method in a finite element analysis. The implicit integration algorithm is implemented in the finite element code Abaqus through the user-defined material subroutine UMAT. The validation of the proposed model is performed on S2/8552 glass/epoxy off-axis block composite specimens subjected to compression at various strain rates. It is shown that the strain rate-dependent response of composite materials is well captured and the predicted results agree well with the experimental data reported in the literature. © 2016 Elsevier Ltd.

Jin Z.,South China University of Technology | Han S.-Y.,South China University of Technology | Zhang L.,South China University of Technology | Zheng S.-P.,South China University of Technology | And 2 more authors.
Bioresource Technology | Year: 2013

Lipase-displaying whole cells appear to be efficient biocatalysts because of their low preparation costs and simple recycling procedure. The combined utilization of. Candida antarctica lipase B (CALB) and. Rhizomucor miehei lipase (RML), separately displayed on. Pichia pastoris whole cells, to produce biodiesel in co-solvent media was investigated. A response surface methodology incorporating a D-optimal design was employed to obtain the optimum reaction conditions for methyl ester (ME) synthesis. The synergistic effect of the two displayed lipases and the use of tert-butanol and isooctane as the co-solvent media were found to significantly improve the transesterification reaction. Scaled-up reactions using various types of feedstock were carried out in a 0.5-l stirred reactor under optimum conditions, affording ME yields over 90% in 12 h. Moreover, the ME yields remained above 85% after 20 repeated batch cycles. In conclusion, this biocatalyst affords a promising route to efficient biodiesel production. © 2012 Elsevier Ltd.

Chen H.,Harvard University | Chen H.,Jinan University | Shen J.,Harvard University | Choy E.,Harvard University | And 2 more authors.
Cancer Treatment Reviews | Year: 2016

Sarcomas are a group of cancers that arise from transformed cells of mesenchymal origin. They can be classified into over 50 subtypes, accounting for approximately 1% of adult and 15% of pediatric cancers. Wide surgical resection, radiotherapy, and chemotherapy are the most common treatments for the majority of sarcomas. Among these therapies, chemotherapy can palliate symptoms and prolong life for some sarcoma patients. However, sarcoma cells can have intrinsic or acquired resistance after treatment with chemotherapeutics drugs, leading to the development of multidrug resistance (MDR). MDR attenuates the efficacy of anticancer drugs and results in treatment failure for sarcomas. Therefore, overcoming MDR is an unmet need for sarcoma therapy. Certain protein kinases demonstrate aberrant expression and/or activity in sarcoma cells, which have been found to be involved in the regulation of sarcoma cell progression, such as cell cycle, apoptosis, and survival. Inhibiting these protein kinases may not only decrease the proliferation and growth of sarcoma cells, but also reverse their resistance to chemotherapeutic drugs to subsequently reduce the doses of anticancer drugs and decrease drug side-effects. The discovery of novel strategies targeting protein kinases opens a door to a new area of sarcoma research and provides insight into the mechanisms of MDR in chemotherapy. This review will focus on the recent studies in targeting protein kinase to reverse chemotherapeutic drug resistance in sarcoma. © 2015 Elsevier Ltd

Liu H.,Jinan University
International Journal of Emerging Technologies in Learning | Year: 2013

Based on the topic models database and time density of caption's dialogue, designed a adaptive, iterative incremental learning algorithm, which could meanwhile carry out topic detection, words clustering and calculating words' used degree. Lastly, based on words' used degree, by calculating degree of difficulty of caption's dialogue, graded videos' corresponding to captions, constructed a large multimedia database for oral Chinese teaching.

Yi X.,University of Electronic Science and Technology of China | Chen X.,University of Electronic Science and Technology of China | Sharma D.,University of Electronic Science and Technology of China | Li C.,State Key Laboratory of Optical Communication Technology and Networks | And 4 more authors.
Optics Express | Year: 2014

Digital coherent superposition (DCS) provides an approach to combat fiber nonlinearities by trading off the spectrum efficiency. In analogy, we extend the concept of DCS to the optical OFDM subcarrier pairs with Hermitian symmetry to combat the linear and nonlinear phase noise. At the transmitter, we simply use a real-valued OFDM signal to drive a Mach-Zehnder (MZ) intensity modulator biased at the null point and the so-generated OFDM signal is Hermitian in the frequency domain. At receiver, after the conventional OFDM signal processing, we conduct DCS of the optical OFDM subcarrier pairs, which requires only conjugation and summation. We show that the inter-carrier-interference (ICI) due to phase noise can be reduced because of the Hermitain symmetry. In a simulation, this method improves the tolerance to the laser phase noise. In a nonlinear WDM transmission experiment, this method also achieves better performance under the influence of cross phase modulation (XPM). © 2014 Optical Society of America.

Xu C.L.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To investigate the inhibitory effect of TBN on rabbit platelet aggregation in vitro and in vivo, and compare with tetramethylpyrazine (TMP). To seek out the maximum of platelet aggregation (expressed in percentage) within 5 minutes which was induced by ADP, PAF and AA according to the Born turbidimetric method with a Platelet-Aggregometer. TBN significantly inhibited platelet aggregation induced by ADP, PAF and AA both in vitro and in vivo. TBN was more active than TMP. TBN has significant activity inhibiting platelet aggregation induced by ADP, PAF and AA in vitro and in vivo.

Caucheteur C.,University of Mons | Guo T.,Jinan University | Albert J.,Carleton University
Analytical and Bioanalytical Chemistry | Year: 2015

This paper presents a brief overview of the technologies used to implement surface plasmon resonance (SPR) effects into fiber-optic sensors for chemical and biochemical applications and a survey of results reported over the last ten years. The performance indicators that are relevant for such systems, such as refractometric sensitivity, operating wavelength, and figure of merit (FOM), are discussed and listed in table form. A list of experimental results with reported limits of detection (LOD) for proteins, toxins, viruses, DNA, bacteria, glucose, and various chemicals is also provided for the same time period. Configurations discussed include fiber-optic analogues of the Kretschmann–Raether prism SPR platforms, made from geometry-modified multimode and single-mode optical fibers (unclad, side-polished, tapered, and U-shaped), long period fiber gratings (LPFG), tilted fiber Bragg gratings (TFBG), and specialty fibers (plastic or polymer, microstructured, and photonic crystal fibers). Configurations involving the excitation of surface plasmon polaritons (SPP) on continuous thin metal layers as well as those involving localized SPR (LSPR) phenomena in nanoparticle metal coatings of gold, silver, and other metals at visible and near-infrared wavelengths are described and compared quantitatively.[Figure not available: see fulltext.] © 2015 Springer-Verlag Berlin Heidelberg

Dai J.F.,Jinan University
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2010

To describe and analyze the prevalence and epidemiological features of people with disability caused by injury in the Chinese population, and to provide scientific basis for developing the prevention and control programs on injuries. Statistics and intervention measures were used to analyze the data from the Second China National Sample Survey on injury-caused Disability. Cluster analysis was used to analyze the differences in regions. The overall prevalence of disability caused by injury was 99.68/10 000 which occupied 15.59% of all the disability, with multiple disability excluded. Physical disability and hearing disability accounted for 65.59% and 23.35% of all the injury-caused disability respectively, while those ranked Grade IV and III making up the majority (55.14% and 25.83%) of the disability, respectively. There were significant differences in the distribution of injury-caused disability among different age groups (χ(2) = 23 106.14, P < 0.001) and gender (χ(2) = 2161.74, P < 0.001). Ageing and being males having a higher risk on injury-caused disability. Five clusters were identified by cluster analysis, in which injury-caused disability were severe in the fifth (Sichuan province) and the fourth clusters (Hunan, Chongqing, Guizhou, Ningxia province). Problems discovered by injury-caused disability in the Chinese population should not be ignored. Both physical and hearing disabilities appeared to be the two main types of disability while age, gender and region were related to injury-caused disability. Targeted strategies should be developed to decrease the injury-caused disability in China.

Liu J.,Hong Kong Polytechnic University | Guo N.,Hong Kong Polytechnic University | Li Z.,Jinan University | Yu C.,National University of Singapore | Lu C.,Hong Kong Polytechnic University
Optics Letters | Year: 2013

A microwave photonic filter with the highest reported quality factor (Q) value of 4895.31 is proposed and experimentally demonstrated by using two cascaded infinite impulse response (IIR) filters. Each IIR filter comprises both optical and electronic signals in a feedback loop and thus the loop length can be reduced without the need to consider the light coherence length. The Vernier effect enables a significant improvement of the free spectral ranges and Q values of the cascaded filter. The Q value of the proposed microwave photonic filter can be changed when the loop lengths of two cascaded filters are carefully adjusted. In addition, for a fixed Q, the frequency response of the filter can also be tuned by adjusting the bias of the Mach-Zehnder modulator in each loop. © 2013 Optical Society of America.

Fei L.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Dong S.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Xue L.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Liang Q.,Jinan University | Yang W.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research
Energy Policy | Year: 2011

This paper applies the panel unit root, heterogeneous panel cointegration and panel-based dynamic OLS to re-investigate the co-movement and relationship between energy consumption and economic growth for 30 provinces in mainland China from 1985 to 2007. The empirical results show that there is a positive long-run cointegrated relationship between real GDP per capita and energy consumption variables. Furthermore, we investigate two cross-regional groups, namely the east China and west China groups, and get more important results and implications. In the long-term, a 1% increase in real GDP per capita increases the consumption of energy by approximately 0.48-0.50% and accordingly increases the carbon dioxide emissions by about 0.41-0.43% in China. The economic growth in east China is energy-dependent to a great extent, and the income elasticity of energy consumption in east China is over 2 times that of the west China. At present, China is subject to tremendous pressures for mitigating climate change issues. It is possible that the GDP per capita elasticity of carbon dioxide emissions would be controlled in a range from 0.2 to 0.3 by the great effort. © 2010 Elsevier Ltd.

Ouyang D.,Jinan University | Xu W.,City University of Hong Kong | Lo T.Y.,City University of Hong Kong | Sham J.F.C.,City University of Hong Kong
Construction and Building Materials | Year: 2011

Making use of industrial by-product or waste clay to partially replace cement in concrete has greatly contributed to sustainable development of environment. This study investigated the optimal activation condition for producing high reactivity metakaolin (MK) by using kaolin by-products (KB) from paper industry. Initially, the material properties of KB were analyzed in this study and the results indicated its great potential to be treated by calcination to be a very effective pozzolan. Afterwards, MK samples produced from different activation treatments of KB, including different calcining temperature, calcining duration, initial temperature rise rime and grinding particle size, were applied in mortar to determine their pozzolanic effect. Results indicated that the optimal activation condition for KB to high reactivity MK conversion is 2-h calcining duration at 750 °C and with 7 μm grinding particle size. Finally, compressive and flexural strengths of mortar samples produced by replacing cement with 0-25% MK content were tested and the results showed that 15% was the optimal cement replacement level of MK and the mortar so produced exhibited 20% improvement in compressive strength compared to control mortar. © 2010 Elsevier Ltd. All rights reserved.

Ma Y.,Northwest University, China | Qiao X.,Northwest University, China | Guo T.,Jinan University | Wang R.,Northwest University, China | And 5 more authors.
Optics Letters | Year: 2012

A novel reflective refractometer based on a thin-core fiber (TCF) sandwiched between a leading single-mode fiber (SMF) and a fiber Bragg grating (FBG) imprinted SMF stub was demonstrated. The reflection from the fiber stub occurs in two well-defined wavelength bands, corresponding to the Bragg core mode and cladding modes. The TCF section functions as a tailorable bridge between the FBG core mode reflection and the surrounding refractive index (SRI). Linear response with enhanced sensitivity of 133.26 dB/refractive index unit for temperature-immune SRI measurement within the biologically desirable sensing range of 1.33-1.41 has been achieved via cost-effective power detection. © 2012 Optical Society of America.

Rong J.,Jinan University | Niu Z.,CAS Technical Institute of Physics and Chemistry | Lee L.A.,University of South Carolina | Wang Q.,University of South Carolina
Current Opinion in Colloid and Interface Science | Year: 2011

Viruses, as typical bionanoparticles from nature, possess many superb properties, such as exquisite symmetry, uniformity of size and shape, well-characterized surface chemistry that governs the potential interparticle interactions. These features make viral particles ideal building blocks for self-assembly studies and novel materials development. This review outlines some of the recent research activities in the area of controlled self-assembly of viruses and its potential use as materials. One particular application of the assembled viruses, especially for biomaterials, is also briefly discussed. © 2011 Elsevier Ltd.

Jiang G.Y.,Jinan University
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2012

To explore the characters of lung injury induced by tin dusts and to provide the diagnosis evidence of tin pneumoconiosis. Forty SD rats were randomly divided into four groups: the group exposed to tin dusts from smelting workshop, the group exposed to tin dusts from tin refining workshop, the positive control group exposed to standard quartz dusts and the negative control group exposed to saline. The pathological changes of rat lungs were observed dynamically. In rats exposed to tin dusts, on the 30th day after exposure to tin dusts, the scattered hoar tip size of the spots in surface and section of the lungs were observed, the scattered focal granulomatous inflammation around the small bronchi and dust particles in lung tissue were observed under microscope; on the 90th day after exposure to tin dusts, the granulomatous inflammation increase, the fibroblasts proliferation, collagen fibers formation and positive VG staining were found. There were significant differences, as compared with positive or negative controls (P < 0.05). These pathological changes were basically the characters of specific pathological changes in early tin pneumoconiosis. Non-ferrous metal tin dusts can induce the specific lung injury (granuloma formation) in lung tissue of rats exposed to tin dusts, which fulfilled the diagnostic criteria of specific pathological changes in early tin pneumoconiosis.

Guo Q.Z.,Jinan University
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2010

To evaluate the outcome of health education program on drowning prevention among primary and secondary school children in rural areas. A township was selected and all the students from grade 3 to 5, grade 7 to 8, and grade 10 to 11 were selected to take part in the program. Twelve intervention measures on natural water safety and drowning prevention were carried out for one year. Information was collected using the same questionnaire before and after the intervention program. One year after the intervention was carried out, children's knowledge on drowning prevention improved significantly (13.21, 95%CI: 12.51 - 13.90), and a positive effect was also noticed among boys (12.77, 95%CI: 11.77 - 13.77), girls (13.80, 95%CI: 12.82 - 14.78), and among primary school children(15.51, 95%CI: 14.30 - 16.72), senior high school children (10.78, 95%CI: 9.50 - 12.05) and junior high school children (12.77, 95%CI: 11.84 - 13.71). Overall rates on risk behaviors dropped from 41.4% to 32.2% (by 22.2%) including 15.6% for boys, 35.2% for girls and 13.8%, 29.3%, 26.3% for primary school children, senior high school children, junior high school children, respectively. The incidence rates for non-fatal drowning decreased by 58.9% (from 5.6% to 2.3%). The person-times for treatment on sight, in emergency settings, in outpatient clinic or in the hospitals had a reduction from 399, 78, 36 to 175, 32, 14, respectively. Health education program could improve children's perception on water safety, and reduce their risk behaviors as well as on the incidence of non-fatal drowning in the rural areas.

Zhang C.,Jinan University | Bai X.,University of Massachusetts Dartmouth | Teng J.,Ohio State University | Xuan D.,Ohio State University | Jia W.,City University of Hong Kong
IEEE Journal on Selected Areas in Communications | Year: 2010

Low-connectivity and full-coverage three dimensional Wireless Sensor Networks (WSNs) have many real-world applications. By low connectivity, we mean there are at least k disjoint paths between any two sensor nodes in a WSN, where k ≤ 4. In this paper, we design a set of patterns to achieve 1-, 2-, 3- and 4-connectivity and full-coverage, and prove their optimality under any value of the ratio of communication range rc over sensing range rs, among regular lattice deployment patterns. We further investigate the evolutions among all the proposed lowconnectivity patterns. Finally, we study the proposed patterns under several practical settings. © 2010 IEEE.

Tang P.,Jinan University
2013 IEEE Conference Anthology, ANTHOLOGY 2013 | Year: 2014

Emergencies challenge emergency managers' decision making capabilities for developing unified action plans to ensure effective coordination of multiple responding agencies. In this paper, the HTN planner SHOP2 is extended for adapting to temporal characteristics of emergency response. The experimental results in flood evacuation show that our planner satisfies the specific planning requirements. © 2013 IEEE.

Xu Y.,Guangzhou University | Xu Y.,Jinan University | Miroshnichenko A.E.,Australian National University
Physical Review B - Condensed Matter and Materials Physics | Year: 2014

We propose a dynamically tunable nonreciprocal response for wave propagations by employing nonlinear Fano resonances. We demonstrate that the transmission contrast between waves propagating in opposite directions can be controlled by an excitation signal. In particular, the unidirectional transmission can be flipped at different time of a pulse, resembling a Fano diode with dynamical reconfigurable nonreciprocity. The key mechanism is the interaction between a linear and a nonlinear Fano resonance that allows for the tunable unidirectional wave propagation and ultrahigh transmission contrast ratio. We further present a realistic photonic example which demonstrates the properties of nonreciprocity can be dynamically manipulated using a pump pulse, based on the general theoretical model. © 2014 American Physical Society.

Zhao X.J.,Jinan University
The Tohoku journal of experimental medicine | Year: 2011

Citrin deficiency (CD) is an autosomal recessive disorder with SLC25A13 as causative gene that encodes citrin, the liver-type aspartate/glutamate carrier isoform 2 (AGC2). Neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD), the major CD phenotype at pediatric age, has been previously reported as a self-limiting condition with clinical presentations resolving between 6 months and 1 year of life. We report the prenatal diagnosis of CD in a family with a fatal NICCD proband. The proband was a 10-month-old male presenting cough for 8 days and jaundiced skin 1 day. Physical examination revealed fever, dark jaundiced sclera and skin, hoarse breathing sounds, and hepatosplenomegaly. Laboratory tests uncovered elevated cholestatic indices, increased ammonia, and prolonged activated partial thromboplastin time and prothrombin time, and reduced fibrinogen. Sonography showed the features of liver cirrhosis. Metabolome analysis uncovered large quantity of 4-hydroxyphenyllactate and dicarboxylates in urine and increased citrulline and methionine in blood. The patient passed away due to liver failure at his age of 13.5 months. Mutation analysis revealed him a homozygote of 851del4, a four-base deletion in exon 9 of SLC25A13 gene. On request of the parents who had a second fetus, prenatal diagnosis of CD was performed by PCR-electrophoresis following amniocentesis and amniocyte culture, and demonstrated the fetus a carrier of the same mutation. The fatal proband in the present report has provided clinical evidence challenging the traditional concept on NICCD prognosis. Moreover, as the first trial on CD prenatal diagnosis, this study might open a novel area for clinical management of CD.

Fan X.,Jinan University
Applied Mechanics and Materials | Year: 2014

On the basis of the thought and target of adaptation on the socialist market economy reform and development, this paper discusses the problems about how to develop out domestic accounting computerization with the purpose of complying with the development trend of nowadays' network times. ERP system reflects the advanced financial accounting, management accounting and cost management thought. It can meet the diversified information demands of the information users. The financial system in ERP is integrated, management and decision oriented. It realizes the transformation from the reflection on the financial information after the event to the real-time support on the financial management and decision making, and from the closed single financial management to the integrated comprehensive enterprise management. Therefore, ERP system will be the inexorable trend of our domestic accounting computerization development. © (2014) Trans Tech Publications, Switzerland.

Bai Y.J.,Jinan University
Applied Mechanics and Materials | Year: 2014

With the general progress of information technology, the computer & network technology are generally applied in the tax system of which it will make more rooms for the tax administration and services in implementing the electronization, networking, transparency and efficiency developments. Meanwhile, the computer & network security issues become more prominent and significant in the e-tax system. In the paper, the author puts forward the corresponding solutions and measures by analyzing the network risks of the e-tax applications from the technical and management points. © (2014) Trans Tech Publications, Switzerland.

Xu A.-D.,Jinan University | Wang Y.-J.,Capital Medical University | Wang D.Z.,Illinois College
CNS Neuroscience and Therapeutics | Year: 2013

Background: The last update of the consensus statement on intravenous recombinant tissue plasminogen activator (IV rt-PA) for acute ischemic stroke (AIS) by the Chinese Stroke Therapy Expert Panel was published in 2006. Great progress has been made since then. Aim: To provide another update on the new knowledge of IV rt-PA for AIS since 7 years ago. Method: In summer of 2012, the Chinese Stroke Therapy Expert Panel was reconvened. New publications on the use of IV rt-PA for AIS were reviewed. In addition, all newly published consensus and guidelines from other countries were reviewed. The 2006 version of Chinese Consensus was then updated. Results: There is now clinical evidence to support the use of IV rt-PA between 3 and 4.5 h after the onset with several exclusion criteria. More studies are needed to provide the evidence for IV rt-PA use beyond 4.5 h. There is benefit giving IV rt-PA within 3 h to patients who are older than 80 and in patients with ongoing atrial fibrillation. Patients with INR<1.7 while on warfarin, minor strokes, rapid improving strokes and severe strokes should be treated and can all be benefited from IV rt-PA. Discussion: Since IV rt-PA was initially recommended in 1996, there is now more evidence support its use, efficacy and safety. The treatment time window is also being expanded. More public education on stroke recognition are needed so many stroke patients may benefit from the treatment. Conclusion: The 2013 version of Chinese IV rt-PA consensus contains the most up-to-date information on the use of IV rt-PA for AIS. It will be a useful tool and guideline to provide appropriate thrombolytic therapy to stroke patients who meet the criteria. © 2013 John Wiley & Sons Ltd.

Wu C.,Hong Kong Polytechnic University | Wu C.,Dalian University of Technology | Guan B.-O.,Jinan University | Lu C.,Hong Kong Polytechnic University | Tam H.-Y.,Hong Kong Polytechnic University
Optics Express | Year: 2011

We proposed and experimentally demonstrated a highly sensitive salinity sensor using a polyimide-coated Hi-Bi photonic crystal fiber Sagnac interferometer based on the coating swelling induced radial pressure. This is the first time to exploit fiber coating induced pressure effect for salinity sensing. The achieved salinity sensitivity is 0.742 nm/(mol/L), which is 45 times more sensitive than that of a polyimide-coated fiber Bragg grating. A bare fiber Bragg grating is incorporated into the fiber loop for temperature compensation. © 2011 Optical Society of America.

Jiang Z.,Jinan University | Reilly J.,Novartis
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

Drug-induced phospholipidosis (PLD) is an excessive accumulation of polar phospholipids within cells as a result of medical treatment. Even though a direct link between in vitro drug-induced PLD and toxicity in humans has not yet been firmly established, the development of PLD during preclinical testing in animals is a recognized problem in the pharmaceutical industry and can delay or abort the development process. Therefore, it is of interest to investigate the potential PLD risk of candidates at an early stage of the drug discovery process. In this work, a high throughput physicochemical approach, which is based on measuring the retention factors of the test compound within several different separation systems, was developed for screening phospholipidosis-inducing potential (PLIP) of pharmaceuticals. The measured retention factors of 36 drugs were compared with literature data on PLIP risk from three different sources. It is clearly shown that there is a statistical correlation between the chromatographic retention parameters of tested drugs and their PLIP risk. In conclusion, the retention factor (logk AOT) observed on a docusate sodium salt (AOT) surfactant vesicle electrokinetic chromatography (EKC) system and the logarithm of the volume of distribution (logV d) calculated from immobilized artificial membrane chromatography at pH 7.4 (CHI IAM 7.4) and HSA binding value (% HSA) can provide primary profile prediction for a large number of drug candidates early in the drug discovery process with minimal resources. The observations are that the higher the value of both logk AOT and logV d, the higher the PLIP risk, and we would recommend this dual approach. © 2011 Elsevier B.V..

Chen J.X.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To study the chemical constituents of the full plant of Lobelia chinensis. The compounds were isolated and purified using several column chromatographic methods. Their structures were elucidated based on their physicochemical properties and spectral data. Twelve compounds were isolated from L. chinensis. Their structures were elucidated as 6,7-dimethoxycoumarin (1), fraxinol (2), 5-hydroxy-7-methoxycoumarin (3), tomentin (4), 3'-hydroxygenkwanin (5), apigenin (6), quercetin (7), luteolin (8), linarin (9), luteolin 3',4'-dimethylether-7-O-beta-D-glucoside (10), isoferulic acid (11), and ethyl ros-marinate (12), respectively. Compounds 1 -5 and 10 -12 are isolated from this plant for the first time.

Xue J.,Jinan University
Journal of Applied Mechanics, Transactions ASME | Year: 2013

When a buckle is initiated in a pipe subjected to external pressure, it will propagate along the longitudinal direction of the pipe if the external pressure is greater than its buckle propagation pressure. For a steady state condition, the propagation is simply considered as the translation of the buckle along the pipeline. This paper presents a unique approach to determine the length of the transition zone in a buckle propagating pipe by analyzing the mechanism of postbuckling of the pipe subjected to the external pressure. Buckling is considered to occur locally in the shell, spreading over a certain length along the longitudinal axis of the shell. The governing equations are derived from the postbuckling theory. Approximate solutions are obtained from the Ritz method, using a plausible function of the flexural displacement created based on Timoshenko's ring solution of the transverse collapse mode. The postbuckling equilibrium path shows that the pipeline experiences unstable collapse until the two opposite points on the inner surface contact each other. The length of the transition zone is found to be proportional to the ratio of (radius)3/2/(thickness)1/2 and is hardly affected by the material properties. The analysis is performed by comparing the obtained results with well-established predictions. © 2013 by ASME.

Zhang W.,Fudan University | Zhang W.,Jinan University | Li N.,Fudan University | Bohinc K.,University of Ljubljana | And 2 more authors.
Physical Review Letters | Year: 2013

Using the techniques of optical microscopy and particle tracking, we measure the correlated diffusion in a monolayer of uniform silica spheres dispersed at a water-air interface. It is found that the correlated motion of the interfacial particles can be well described by two universal response functions, the normalized longitudinal and transverse diffusion coefficients DË(r/r0) and DË(r/r0), where r is the interparticle distance and r0=a(λS/ a)3/2 is a new scaling length, which depends on both the Saffman length λS and particle radius a. The obtained response functions characterize the crossover behavior of the colloidal monolayers from the subphase-dominated three-dimensional hydrodynamics at low surface coverage to the monolayer-dominated 2D hydrodynamics at high concentrations. The surface viscosity ηs(2) of the colloidal monolayer obtained by two-particle rheology compares well with the one-particle measurements. © 2013 American Physical Society.

Wang J.B.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To investigate the protective effect and mechanism of curcumin against ActD/TNF-α-induced synergistically apoptosis in PC12 cells. MTT assay was used to evaluate the optimal concentration of drugs; Hoechst 33258 fluorescent staining to observe apoptosis of PC12 cells, JC-1 was used to detect the mitochondrial membrane potential (MMP), real-time PCR was used to detect the expression of two apoptotic genes: Bcl-1 and Bax. RESUITS: ActD/TNF-α can synergistically reduce viability of PC12 cells(P<0.05), increase the number of cells with pyknosis and karyorrhexis, increase apoptotic rate of cells (P<0.05), decrease MMP in cells, and downregulate expression of Bcl-2(P<0.05). After treating with curcumin(5 μmol/L), survival of PC12 cells was increased(P<0.05), the number of cells with pyknosis and karyorrhexis was reduced, MMP and expression of Bcl-2 were increased(P<0.05). Curcumin can resist the ActD/TNF-α-induced synergistically apoptosis in PC12 cells, the mechanisms of which may be related to an increase in MMP and Bcl-2 expression.

Zheng H.T.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011

To establish a new method which analyzes T cell receptor (TCR) gene rearrangement for identification of T cells acute lymphoblastic leukemia (T-ALL) clone, it will provide the basis for the study of T-ALL including the chromosome translocation involving TCR loci. Total DNA was isolated from peripheral blood mononuclear cells (PBMC) of one case with T-ALL. Using the fine-tiling array comparative genomic hybridization (fine-tiling aCGH) to analyze the genomic DNA differences of the case and control group, we could find the breakpoints and their position in the chromosomes. According to the preliminary results, we could design the specific primers for the positions of the breakpoints relative to sequence. Furthermore, the ligation-mediated PCR (LM-PCR) and sequence analysis were used to identify the TCR gene rearrangement. And TCR gene expression was detected by RT-PCR. The fine-tiling aCGH results of the T-ALL showed that the TCRα/δ locus of chromosome 14 appeared four breakpoints, corresponding to TCR Vδ1, Vδ2, Jδ1 and Jδ2. By LM-PCR, sequencing and sequence analysis, TCR gene of the case of T-ALL was involved in Vδ1Dδ2Dδ3Jδ1, Vδ2Dδ3Jδ2 rearrangement. RT-PCR results also confirmed the expression of these TCR gene rearrangements. The results demonstrated that fine-tiling aCGH and LM-PCR techniques could be used to identify the TCR gene rearrangement as one of the best perfect methods. And it was also a way to find some fusion genes involving in TCR gene.

He Z.,Jinan University
Proceedings of the 2010 IEEE International Conference on Progress in Informatics and Computing, PIC 2010 | Year: 2010

In this paper, a new Wavelet-AR feature for activity recognition from a tri-axial acceleration signals has been proposed. We use Wavelet Transform to decompose the raw accelerometer signals and obtain the decomposed singnals that can efficiently discriminate the different activities. After that we build autoregressive (AR) model for the decomposed signals and extract the AR coefficients as features for activity recognition. As a consequence, Multi-class Support Vector Machines is uesed to distinguish different human activities. The average recognition results for four activities using the proposed Wavelet-AR features are 95.45%, which are better than traditional features. The results show that Wavelet-AR feature obvious discriminates different human activities and it can be extract as an effective feature for the recognition of accelerometer data. ©2010 IEEE.

Liu Z.,Hong Kong Polytechnic University | Wu C.,Hong Kong Polytechnic University | Wu C.,Jinan University | Tse M.-L.V.,Hong Kong Polytechnic University | And 2 more authors.
Optics Letters | Year: 2013

Inthis Letter, we reported on an ultrahigh birefringence photonic crystal fiber (PCF) with a germanium-doped elliptical core, which is fabricated in our lab using the stack-and-draw method. An ultrahigh birefringence of 1.1 × 10-2 is obtained experimentally, which isclosetothe theoretical value of1.4 × 10-2 at the wavelength of1550 nm. Toour knowledge, this is the highest birefringence reportedto date for fabricated index-guiding PCF. Fiber Bragggratings(FBG) were written in the fiber to confirm its ultrahigh birefringence, and we demonstrated the capability to simultaneously measure the FBG's pressure and temperature experimentally. Because of the large separation of the two FBG peaks (>12 nm), such fiber is a promising candidate for a single polarization device. © 2013 Optical Society of America.

Sui W.,Guangxi Key laboratory of Metabolic Diseases Research | Hou X.,Guangxi Key laboratory of Metabolic Diseases Research | Hou X.,Guangxi Normal University | Che W.,Guangxi Key laboratory of Metabolic Diseases Research | And 3 more authors.
Genes and Immunity | Year: 2013

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by the production of autoantibodies directed against nuclear self-Antigens and circulating immune complexes. This results in damages to various organs or systems, including skin, joints, kidneys and the central nervous system. Clinical manifestations of SLE could be diverse, including glomerulonephritis, dermatitis, thrombosis, vasculitis, seizures and arthritis. The complicated pathogenesis and varied clinical symptoms of SLE pose great challenges in the diagnosis and monitoring of this disease. Unfortunately, the etiological factors and pathogenesis of SLE are still not completely understood. It is noteworthy that recent advances in our understanding of the biological omics and emerging technologies have been providing new tools in the analyses of SLE, such as genomics, epigenomics, transcriptomics, proteomics, metabolomics and so on. In this article, we summarize our current knowledge in this field for a better understanding of the pathogenesis, diagnosis and treatment for SLE. © 2013 Macmillan Publishers Limited.

Nie P.-Y.,Jinan University
Computational and Mathematical Organization Theory | Year: 2014

Penalty is a crucial approach to maintain society in order in both the legal and the political philosophies. How to establish a rational and efficient penalty mechanism is exceedingly important in practice in economics and politics and this paper explores the optimal mechanism design of penalty. A penalty under monopoly mechanism design theory is established and developed in this piece of work. By establishing the penalty mechanism design model, this paper finds that stricter punishment can efficiently deter violation of the regulations but can decrease the profits of the monopoly firm at the same time. Furthermore, penalty increases the concavity of the monopoly firm’s profit function, which makes it easier for the firm to make decisions and which means strict penalty results in optimal decisions. We also show that punishment is in general costly, which is highly consistent with the phenomena in practice. © 2013, Springer Science+Business Media New York.

Shim Y.Y.,University of Saskatchewan | Shim Y.Y.,Prairie Tide Chemicals Inc. | Reaney M.J.T.,University of Saskatchewan | Reaney M.J.T.,Prairie Tide Chemicals Inc. | Reaney M.J.T.,Jinan University
Journal of Agricultural and Food Chemistry | Year: 2015

Cyclolinopeptides (CLs) are octa-, nona-, and decapeptides present in flaxseed (Linum usitatissimum L.) that may have immunosuppressive and antitumor activities, but little is known of their pharmacokinetics. Human serum albumin (HSA), the most abundant blood protein, is an important mediator of organic solute flux, and hence when compounds bind this protein, it potentially affects both their availability and efficacy. Quantitative thermodynamic analysis of the interaction of compounds with HSA is important in the development of biomedical applications. A surface plasmon resonance (SPR) biosensor was utilized to reliably determine binding constants for several CLs with HSA. The maximum binding response of [1-9-NαC]-CLA/HSA was almost 20-fold higher than that of [1-8-NαC],[1-MetO]-CLE/HSA. Through analysis of an array of peptides, it was possible to correlate the impact of structural changes on CL binding. The oxidation of sulfur in methionine (Met) residues formed methionine S-oxide (MetO) and reduced binding significantly. Most strikingly, the further oxidation of MetO to S,S-dioxide (MetO2) produced CLs with stronger binding. The large impact on binding by relatively small modifications of methionine containing CLs suggested that small changes in methionine oxidation can disrupt hydrophobic interaction, the predominant intermolecular force stabilizing the complex between CLs and HSA. SPR binding studies may aid in understanding the fate of CLs after consumption of flaxseed or flaxseed products or the development of CLs as drugs or drug carriers. © 2015 American Chemical Society.

Chu J.,South China University of Technology | Xie X.-H.,Jinan University | Yang S.-R.,South China University of Technology | Zhan S.-Z.,South China University of Technology
Inorganica Chimica Acta | Year: 2014

The reaction of 1,3-bis[(2-chloro)benzene]triazene (HL) and [Pd(CH 3CN)4]Cl2 gave a new dinuclear triazenido complex [Pd2L2] 1 (L = 1,3-bis[(2-chloro)benzene]triazene ion). Electrochemcial studies showed that complex 1 is capable of generating hydrogen from acetic acid in CH2Cl2. © 2013 Elsevier B.V. All rights reserved.

Ge R.,Sun Yat Sen University | Sun X.,Jinan University
Metallomics | Year: 2014

Gram-positive Streptococcus species are responsible for millions of cases of meningitis, bacterial pneumonia, endocarditis, erysipelas and necrotizing fasciitis. Iron is essential for the growth and survival of Streptococcus in the host environment. Streptococcus species have developed various mechanisms to uptake iron from an environment with limited available iron. Streptococcus can directly extract iron from host iron-containing proteins such as ferritin, transferrin, lactoferrin and hemoproteins, or indirectly by relying on the employment of specialized secreted hemophores (heme chelators) and small siderophore molecules (high affinity ferric chelators). This review presents the most recent discoveries in the iron acquisition system of Streptococcus species-the transporters as well as the regulators. This journal is © the Partner Organisations 2014.

Chi C.Y.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To study the chemical constituents of Yao Medicine Cissus pteroclada. The compounds were isolated and purified by column chromatography with silica gel, TLC and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis. Six compounds were isolated and identified as beta-sitosterol (I), bergenin (II), 11-O-galloylbergenin (III), 11-O-(4-hydroxy benzoyl) bergenin (IV), gallic acid (V), daucosterol (VI). Compounds III and NIV are obtained from the genus for the first time. All the compounds are isolated from this plant for the first time except the compound II.

You Y.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

To investigate the expression of fractalkine (FKN) in the blood and renal tissues of patients with lupus nephritis and explore its significance. According to the pathological classification, 48 patients with lupus nephritis were divided into mild group (22 cases) and severe group (26 cases), with 26 healthy subjects as the control group. RT-PCR and enzyme-linked immunosorbent assay were employed to detect the expression of FKN mRNA and protein in the blood of the subjects, and FKN expression and localization in the renal tissue of the patients with lupus nephritis were detected using immunohistochemical staining. The patients in both the mild and severe groups showed significantly increased expression of blood FKN mRNA and protein compared with the normal controls, and the increase was more obvious in severe cases (P<0.01). In the renal tissues of the patients, FKN was located mainly in the cytoplasm of the glomerular podocytes and renal tubular epithelial, and the number of positive glomerular cells number was significantly greater in severe cases than in the mild cases (P<0.01); FKN expression in the cortical interstitium did not show a significant difference between the 3 groups. FKN expression in the blood and glomeruli of patients with lupus nephritis is related to the severity of renal pathologies.

Huang Z.,University of Hawaii at Manoa | Huang Z.,Jinan University | Rose A.H.,University of Hawaii at Manoa | Hoffmann P.R.,University of Hawaii at Manoa
Antioxidants and Redox Signaling | Year: 2012

Dietary selenium (]Se), mainly through its incorporation into selenoproteins, plays an important role in inflammation and immunity. Adequate levels of Se are important for initiating immunity, but they are also involved in regulating excessive immune responses and chronic inflammation. Evidence has emerged regarding roles for individual selenoproteins in regulating inflammation and immunity, and this has provided important insight into mechanisms by which Se influences these processes. Se deficiency has long been recognized to negatively impact immune cells during activation, differentiation, and proliferation. This is related to increased oxidative stress, but additional functions such as protein folding and calcium flux may also be impaired in immune cells under Se deficient conditions. Supplementing diets with above-adequate levels of Se can also impinge on immune cell function, with some types of inflammation and immunity particularly affected and sexually dimorphic effects of Se levels in some cases. In this comprehensivearticle, the roles of Se and individual selenoproteins in regulating immune cell signaling and function are discussed. Particular emphasis is given to how Se and selenoproteins are linked to redox signaling, oxidative burst, calcium flux, and the subsequent effector functions of immune cells. Data obtained from cell culture and animal models are reviewed and compared with those involving human physiology and pathophysiology, including the effects of Se levels on inflammatory or immune-related diseases including anti-viral immunity, autoimmunity, sepsis, allergic asthma, and chronic inflammatory disorders. Finally, the benefits and potential adverse effects of intervention with Se supplementation for various inflammatory or immune disorders are discussed. © 2012, Mary Ann Liebert, Inc.

Su H.-C.,CAS Guangzhou Institute of Geochemistry | Ying G.-G.,CAS Guangzhou Institute of Geochemistry | Tao R.,CAS Guangzhou Institute of Geochemistry | Tao R.,Jinan University | And 3 more authors.
Environmental Pollution | Year: 2012

Antibiotic susceptibility, detection of sul gene types and presence of class 1, 2 and 3 integrons and gene cassettes using PCR assays were investigated in 3456 Escherichia coli isolates obtained from 38 sampling sites of the Dongjiang River catchment in the dry and wet seasons. 89.1% of the isolates were resistant and 87.5% showed resistance to at least three antibiotics. sul2 was detected most frequently in 89.2% of 1403 SXT-resistant isolates. The presence of integrons (class 1 and 2) was frequently observed (82.3%) while no class 3 integron was found. In these integrons, 21 resistance genes of 14 gene cassette arrays and 10 different families of resistance genes were identified. Three gene cassette arrays, aac(6')-Ib-cr-aar-3-dfrA27-aadA16, aacA4-catB3-dfrA1 and aadA2-lnuF, were detected for the first time in surface water. The results showed that bacterial resistance in the catchment was seriously influenced by human activities, especially discharge of wastewater. © 2012 Elsevier Ltd. All rights reserved.

Cai Yu.,Jinan University | Cai T.,Liaoning University | Chen Y.,Ontario Cancer Institute
Journal of Cellular Biochemistry | Year: 2014

Osteosarcoma is the most common malignant bone tumor in children and adolescents. Although pathologic characteristics of this disease are clear and well established, much remains to be understood about this tumor, particularly at the molecular signaling level. Secreted signaling molecules of the Wnt family have been widely investigated and found to play a central role in controlling embryonic bone development, bone mass, and postnatal bone regeneration. A variety of studies also suggest that Wnt signaling pathway is closely associated with bone malignancies, including breast or prostate cancer induced bone metastasis, multiple myeloma, as well as osteosarcoma. Here, we provide an overview of the role of Wnt signaling pathway in osteosarcoma development and progression, highlighting the aberrant activation of Wnt pathway in this bone malignancy. We also discuss the potential therapeutic applications for the treatment of osteosarcoma targeting Wnt pathway. J. Cell. Biochem. 115: 625-631, 2014. © 2013 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.

Hu Y.S.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To investigate the biotransformation of artemisinic acid by cell suspension cultures of Cephalotaxus fortunei and Artemisia annua. Artemisinic acid was added into to the media of the suspension cells of Cephalotaxus fortunei and Artemisia annua in their logarithmic growth phase. The biotransfromed product was detected with HPLC and isolated by silica gel column, Sephadex LH20 and ODS chromatography methods. The chemical structure of biotransformed product was elucidated on the basis of physical-chemical properties and spectroscopic data. Otherwise, the influence of co-cultured time on conversion ratio was investigated with HPLC. One biotransformed product, 3-alpha-hydroxyartemisinic acid, was obtained after two days of artemisinic acid administration to the suspension cells of Cephalotaxus fortunei and Artemisia annua. The optimal co-cultured time in suspension cells of Cephalotaxus fortunei was 2 days with the highest biotransformation rate of 8.42%, and in the case of Artemisia annua, it was 3 days and 3.95% respectively. It was the first time for the biotransformation of artemisinic acid to 3-alpha-hydroxyartemisinic acid by using cell suspension cultures of Cephalotaxus fortunei and Artemisia annua.

This paper investigates the common movements of commodity sectors in China as well as the economic underpinnings of the comovements. We employ a Bayesian dynamic latent factor model to disentangle the common and idiosyncratic sector-specific factors of the prices of a group of China's commodity sectors: petrochemicals, grains, energy, non-ferrous metals, oils & fats, and softs. The results indicate that the common factor accounts for a significant portion of the fluctuations of China's commodity sectors, providing evidence of the strong commodity sector comovements in China. We further use a VAR model to link the common movements across China's commodity sectors to the underlying determinants, including global oil price shocks and domestic macroeconomic fluctuations. We find that the global oil price shocks have strong effects on the common movements across commodity sectors in China in addition to its domestic macroeconomic fluctuations at long horizons. However, at short horizons, the common movements across commodity sectors in China respond more strongly to the global oil shocks than to its domestic macroeconomic fluctuations. © 2015 Elsevier Ltd.

Ai B.-Q.,South China Normal University | Zhong W.-R.,Jinan University | Hu B.,Hong Kong Baptist University | Hu B.,University of Houston
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2011

We study heat conduction through one-dimensional homogeneous lattices in the presence of the nonlinear on-site potentials containing the bounded and unbounded parts, and the harmonic interaction potential. We observe the occurrence of double negative differential thermal resistance (NDTR); namely, there exist two regions of temperature difference, where the heat flux decreases as the applied temperature difference increases. The nonlinearity of the bounded part contributes to NDTR at low temperatures and NDTR at high temperatures is induced by the nonlinearity of the unbounded part. The nonlinearity of the on-site potentials is necessary to obtain NDTR for the harmonic interaction homogeneous lattices. However, for the anharmonic homogeneous lattices, NDTR even occurs in the absence of the on-site potentials, for example, the rotator model. © 2011 American Physical Society.

Wang Y.,Jinan University | Smith Jr. W.O.,Virginia Institute of Marine Science | Wang X.,Ocean University of China | Li S.,South China Normal University
Geophysical Research Letters | Year: 2010

Recent investigations into the role of carbon dioxide on phytoplankton growth and composition have clearly shown differential effects among species and assemblages, suggesting that increases in oceanic CO2 may play a critical role in structuring lower trophic levels of marine systems in the future. Furthermore, alarming increases in the occurrence of harmful algal blooms (HABs) in coastal waters have been observed, and while not uniform among systems, appear in some manner to be linked to human impacts (eutrophication) on coastal systems. Models of HABs are in their infancy and do not at present include sophisticated biological effects or their environmental controls. Here we show that subtle biological responses occur in the HAB species Phaeocystis globosa Scherffel as a result of CO2 enrichment induced by gentle bubbling. The alga, which has a polymorphic life history involving the formation of both colonies and solitary cells, exhibited altered growth rates of colonial and solitary forms at [CO2] of 750 ppm, as well as increased colony formation. In addition, substantial modifications of elemental and photosynthetic constituents of the cells (C cell-1, N cell -1, potential quantum yield, chl a cell-1) occurred under elevated CO2 concentrations compared to those found at present CO2 levels. In contrast, other individual and population variables (e.g., colony diameter, total chlorophyll concentration, carbon/nitrogen ratio) were unaffected by increased CO2. Our results suggest that predictions of the future impacts of Phaeocystis blooms on coastal ecosystems and local biogeochemistry need to carefully examine the subtle biological responses of this alga in addition to community and ecosystem effects. Copyright © 2010 by the American Geophysical Union.

Hou D.-F.,Central China Normal University | Liu H.,Jinan University | Ren H.-C.,Rockefeller University
Physical Review D - Particles, Fields, Gravitation and Cosmology | Year: 2012

The two-loop contributions to the chiral vortical conductivity are considered. The Kubo formula together with the anomalous Ward identity of the axial vector current suggest that there may be a nonzero correction to the coefficient of the T2 term of the conductivity. © 2012 American Physical Society.

Chen X.,Jinan University | Xu J.,Baiyun District Bureau of Justice
Pattern Recognition | Year: 2016

Gait is an important biometric which can operate from a distance without subject cooperation. However, it is easily affected by changes in covariate conditions (carrying, clothing, view angle, walking speed, random noise etc.). It is hard for training set to cover all conditions. Bipartite ranking model has achieved success in gait recognition without assumption of subject cooperation. We propose a multi-view hypergraph learning re-ranking (MHLRR) method by integrating multi-view hypergraph learning (MHL) with hypergraph-based re-ranking framework. Sparse coding re-ranking (SCRR) and MHLRR are integrated under the graph-based framework to get a model. We define it as the sparse coding multi-view hypergraph learning re-ranking (SCMHLRR) method, which makes our approach achieve higher recognition accuracy under a genuine uncooperative setting. Extensive experiments demonstrate that our approach drastically outperforms existing ranking based methods, achieving good increase in recognition rate under the most difficult uncooperative settings. © 2015 Elsevier Ltd. All rights reserved.

Zeng H.L.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To establish two-dimensional electrophoresis (2-DE) pattern for proteome analysis on K562 cells before and after treatment with 6-gingerol, and mass-spectrometry was applied to identify and analyze the differentially expressed proteins. K562 cells were treated with 6-gingerol. Cell proliferation was analyzed by CCK-8 assay. Total protein of K562 cells was extracted by 2D-DIGE and then imaged by SDS gel scanning. The differentially expressed proteins were identified using Imagine Master 2D Platinum 6.0 software and functionally classified by MALDI and MALDI-TOF MS. 6-gingerol could significantly inhibit K562 cells proliferation and the efficacy was concentration and dose-dependent. After being treated for 24, 48, 72 h, IC50 was 22.86, 15.75, 11.18 microg/mL, respectively. 42 differentially expressed proteins were identified, including 19 up-regulated expressed proteins and 10 down-regulated expressed proteins. Proteomic technique can be used to screen multiple proteins associate with the anti-leukemia effect of 6-gingerol, involving some important proteins related to oxidative stress, cell cycle regulation, apoptosis signal transduction, biosynthesis and glycometabolism.

Bai Z.Q.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2010

AIM: To explore the the effect of gastrodine on hepatitic cell line L02 injury induced by ethanol. METHODS: The growing L02 cells were treated with different concentrations of alcohol. For screening the proper concentration of alcohol, the proliferation of the cells were measured by MTT colorimetry. The level of reactive oxygen species (ROS) and change of mitochondrial membrane potential in cells were tested by flow cytometry. Reversed-phase high-performance liquid chromatography (RP-HPLC) was applied to assay ATP content in cells. RESULTS: The cells were injured when treated with 25 mL/L alcohol for 36 h. Compared with the control group , the ROS level in cells markedly increased while mitochondrial membrane potential and ATP content in cells significantly decreased (P<0.01). Gastrodine obviously inhibited cell damage, reduced ROS level, and increased mitochondrial membrane potential and ATP content in cells. CONCLUSION: Above results suggest that gastrodine may suppress cellular injury and lipid peroxidation induced by ethanol, improve mitochondrial function and raise ATP level, which play an important role in protecting hepatocytes.

Zeng H.L.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To observe the effects of 6-gingerol on reactive oxygen species (ROS) and mitochondrial membrane potential(deltapsim) of chronic myeloid leukemia K562 cells and human acute T lymphoblastic leukemia MOLT4 cells, to investigate the role of mitochondrial pathway in the signal transduction of leukemia cell. With different concentrations of 6-gingerol treatment, using 2,7-dichloro fluoresceinciactate (DCFH-DA) as ROS probe, rhodamine-123 as deltapsim probe, the levels of ROS and deltapsim of K562 cells and MOLT4 cells were tested by flow cytomentry. After treated with 6-gingerol, the ROS levels of K562 cells were significantly higher than control group (P < 0.01), while the deltapsim were significantly lower than control group (P < 0.01), and the ROS levels of MOLT4 cells were significantly higher than control group (P < 0.05). 6-gingerol can significantly increase ROS levels of K562 cells and MOLT4 cells, decrease deltapsim of K562 cells,induce apoptosis of leukemia cells by mitochondrial pathway.

Jia H.,Jinan University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2010

To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms. siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology. 1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%. siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.

The monofunctional and bifunctional bindings of the potential anticancer drug trans-isopropylaminedimethylaminedichloroplatinum (trans-IPADMADP) and its cis isomer to purine base in DNA are explored by using density functional theory and IEF-PCM solvation models. The computed lowest free energy barrier in the aqueous solution is 14.0/11.6 kcal/mol (from trans-Pt-chloroaqua complex to trans-/cis-monoadduct) for guanine(G), and 11.7/13.3 kcal/mol (from trans-Pt-chloroaqua complex to trans-/cis-monoadduct) for adenine(A). Our calculations demonstrate that the trans reactant complexes (or isolated reactants) can generate trans- or cis-monoadducts via similar trigonal bipyramidal transition state structures, suggesting that the monoadducts can subsequently close to form the bifunctional intrastrand Pt-DNA adducts and simultaneously distort DNA in the similar way as cisplatin. Our calculations show that Pt(isopropylamine)(dimethylamine)G 2 2+ head-to-head path has the lowest free energy of activation at 17.6 kcal/mol, closely followed by the Pt(isopropylamine)(dimethylamine)GA 2+ head-to-head path at 19.6 kcal/mol when the monofunctional cis-Pt-G complex serves as the reactant; while the Pt(isopropylamine)(dimethylamine)G 2 2+ head-to-tail adduct has the lowest barrier of 20.5 kcal/mol, closely followed by the Pt(isopropylamine)(dimethylamine)GA 2+ head-to-tail adduct at 23.0 kcal/mol if the monofunctional trans-Pt-G complex is the reactant. The calculated relatively lower activation energy barrier than that of cisplatin theoretically confirm that trans-[PtCl 2(isopropylamine)(dimethylamine)] is a potential anticancer drug as described by experiment. © 2011 Elsevier B.V. All rights reserved.

Fang R.,Sun Yat Sen University | Zhang G.,Sun Yat Sen University | Guo Q.,Sun Yat Sen University | Ning F.,Sun Yat Sen University | And 3 more authors.
Cancer Letters | Year: 2013

Nodal, an important embryonic morphogen, has been reported to function in tumorigenesis. Here we report for the first time that Nodal promotes malignancy by inducing epithelial-mesenchymal transition (EMT) in B16 murine melanoma. These cells displayed increased migration and invasion abilities upon treating with Nodal, accompanying with typical phenotype changes of EMT. In contrast, Nodal knockdown or blocking Nodal signaling using a specific antagonist SB431542 repressed the EMT phenotype as well as reduced cell motility and invasiveness. Treatment with Nodal also induced expression of transcription factor Snail. Snail knockdown abolished the Nodal-induced EMT in B16 cells. We further show that Snail expression is mediated by the Nodal-regulated AKT/GSK-3β signaling. Taken together, these results revealed that Nodal promotes the aggressive phenotype of B16 murine melanoma cells by inducing EMT via up-regulation of Snail. This study provides a better understanding of Nodal function in melanoma, and suggests a potential novel target for clinical therapeutic research. © 2013 Elsevier Ireland Ltd.

Li B.,University of Hong Kong | Li B.,Center for Cancer Research | Tsao S.W.,University of Hong Kong | Tsao S.W.,Center for Cancer Research | And 8 more authors.
Clinical Cancer Research | Year: 2014

Purpose: To investigate the autocrine/endocrine role of Id1-induced insulin-like growth factor-II (IGF-II) in esophageal cancer, and evaluate the potential of IGF-II-and IGF-type I receptor (IGF-IR)-targeted therapies. Experimental Design: Antibody array-based screening was used to identify differentially secreted growth factors from Id1-overexpressing esophageal cancer cells. In vitro and in vivo assays were performed to confirm the induction of IGF-II by Id1, and to study the autocrine and endocrine effects of IGF-II in promoting esophageal cancer progression. Human esophageal cancer tissue microarray was analyzed for overexpression of IGF-II and its correlation with that of Id1 and phosphorylated AKT (p-AKT). The efficacy of intratumorally injected IGF-II antibody and intraperitoneally injected cixutumumab (fully human monoclonal IGF-IR antibody) was evaluated using in vivo tumor xenograft and experimental metastasis models. Results: Id1 overexpression induced IGF-II secretion, which promoted cancer cell proliferation, survival, and invasion by activating AKT in an autocrine manner. Overexpression of IGF-II was found in 21 of 35 (60%) esophageal cancer tissues and was associated with upregulation of Id1 and p-AKT. IGF-II secreted by Id1-overexpressing esophageal cancer xenograft could instigate the growth of distant esophageal tumors, as well as promote metastasis of circulating cancer cells. Targeting IGF-II and IGF-IR had significant suppressive effects on tumor growth and metastasis in mice. Cixutumumab treatment enhanced the chemosensitivity of tumor xenografts to fluorouracil and cisplatin. Conclusions: The Id1-IGF-II-IGF-IR-AKT signaling cascade plays an important role in esophageal cancer progression. Blockade of IGF-II/IGF-IR signaling has therapeutic potential in the management of esophageal cancer. © 2014 American Association for Cancer Research.

Chen A.,Jinan University | Groenewold N.,University of Western Australia
Annals of Regional Science | Year: 2013

Fiscal decentralisation has been an important part of the restructuring of China's economy over the past three decades. Yet, there has been only limited analysis of the way in which decentralisation in China might affect variables such as welfare, output and income at the aggregate level and none at the regional level. This paper makes a start at filling this gap by analysing the aggregate and regional effects of various policies which aim to change the balance between fiscal activities of the national and regional governments. For this, we use a small theoretical model designed to capture some of the features of the Chinese economy, and we solve it numerically using a parameterisation based on Chinese data. We analyse the effects of four different shocks; all of them involve a transfer of resources to the regional governments financed by a cut in central government expenditure, but they differ in the way in which regional governments use the additional funds: (1) they adjust expenditure on the consumption good, (2) they adjust infrastructure expenditure, (3) they maximise the size of their own budget, and (4) they maximise the welfare of the representative citizen. We find that the aggregate economic effects of decentralisation depend on the precise nature of the policy and that aggregate benefits may often mask a deterioration in the inter-regional distribution of those benefits. © 2013 Springer-Verlag Berlin Heidelberg.

Nie P.-Y.,Jinan University
Energy and Environment | Year: 2013

Based on existing pollution emission policies, this study addresses the relationship between innovation and energy input along with energy price. Both energy demand and innovative investment are characterized in this work. First, this paper argues that higher energy prices results in lower innovation investment in reduction of emission, higher innovation in production, lower energy demand and lower consumer surplus. Second, the increased effects of emissions on consumers yield lower innovation investment, lower energy demand and lower consumer surplus. Finally, policies for controlling emissions are also discussed in this study. Stricter policies for controlling emissions yield more innovative investments to reduce emissions and less investment to improve production efficiency, resulting in lower demand for energy and lower demand for and higher prices of products. In theory, this study develops the theory about multi-dimensional innovations and captures the relationship between innovative investments to reduce emissions and innovation to improve production efficiency.

Xiong Y.Q.,Jinan University | Hua B.,South China University of Technology
Advanced Materials Research | Year: 2014

In this paper, a cryogenic air separation process with LNG cold energy utilization is proposed to produce liquid nitrogen and high pressure pure oxygen gas economically. To reduce the electric energy consumption of air separation products, liquid nitrogen have been produced by condensing the separated pure nitrogen gas with LNG cold energy utilization, and the recycled nitrogen is served to transfer cold energy from LNG stream to cool off air stream in the proposed cryogenic air separation process. The specifications of streams and the major equipments of the air separation process are simulated with Aspen Plus software and the main parameters analysis are performed. The results show that the energy consumption of the proposed air separation process with LNG cold energy utilization decreased about 58.2% compared with a conventional cryogenic air separation process. The compressed pressure of recycled nitrogen has a big impact on the cost of air separation products and utilization efficiency of LNG cold energy. The LNG cold energy could be fully utilized when the recycled nitrogen has been compressed to above 6.5MPa. © (2014) Trans Tech Publications, Switzerland.

Lu Y.-Y.,South China Normal University | Chen T.-S.,Sun Yat Sen University | Wang X.-P.,Jinan University | Li L.,Sun Yat Sen University
Journal of Biomedical Optics | Year: 2010

Dihydroartemisinin (DHA), a front-line antimalarial herbal compound, has been shown to possess promising anticancer activity with low toxicity. We have previously reported that DHA induced caspase-3-dependent apoptosis in human lung adenocarcinoma cells. However, the cellular target and molecular mechanism of DHAinduced apoptosis is still poorly defined. We use confocal fluorescence microscopy imaging, fluorescence resonance energy transfer, and fluorescence recovery after photobleaching techniques to explore the roles of DHA-elicited reactive oxygen species (ROS) in the DHAinduced Bcl-2 family proteins activation, mitochondrial dysfunction, caspase cascade, and cell death. Cell Counting Kit-8 assay and flow cytometry analysis showed that DHA induced ROS-mediated apoptosis. Confocal imaging analysis in a single living cell and Western blot assay showed that DHA triggered ROS-dependent Bax translocation, mitochondrial membrane depolarization, alteration of mitochondrial morphology, cytochrome c release, caspase-9, caspase-8, and caspase-3 activation, indicating the coexistence of ROS-mediated mitochondrial and death receptor pathway. Collectively, our findings demonstrate for the first time that DHA induces cell apoptosis by triggering ROS-mediated caspase-8/Bid activation and the mitochondrial pathway, which provides some novel insights into the application of DHA as a potential anticancer drug and a new therapeutic strategy by targeting ROS signaling in lung adenocarcinoma therapy in the future. © 2010 Society of Photo Optical Instrumentation Engineers.

Ding D.-P.,Southern Medical University | He X.-F.,Southern Medical University | Zhang Y.,Jinan University
Breast Cancer Research and Treatment | Year: 2011

The xeroderma pigmentosum group G (XPG or ERCC5) and group F (XPF or ERCC4) play an important role in DNA repair, and produce dual incision 3 and 5 to the damaged nucleotide fragment. Several polymorphisms in the XPF and XPG gene have been described, including the commonly occurring Asp1104His in XPG and Arg415Gln in XPF. The published data on the association between these polymorphisms and breast cancer remained controversial. This meta-analysis of literatures was performed to derive a more precise estimation of the relationship. A total of 17 studies were identified to the meta-analysis, including 5,235 cases and 5,685 controls for XPG Asp1104His (from ten studies) and 3,910 cases and 3,985 controls for XPF Arg415Gln (from seven studies). Overall, no significantly elevated breast cancer risk was found in all genetic models when all studies were pooled into the meta-analysis (for XPG Asp1104His Asp/His vs. Asp/Asp: OR 1.02, 95% CI 0.94-1.11; His/His vs. Asp/Asp: OR 0.96, 95% CI 0.83-1.11; dominant model: OR 1.01, 95% CI 0.94-1.09; and for XPF Arg415Gln Arg/Gln vs. Arg/Arg: OR 1.00, 95% CI 0.89-1.12; Gln/Gln vs. Arg/Arg: OR 2.40, 95% CI 0.62-9.22; dominant model: OR 1.03, 95% CI 0.90-1.18). In stratified analyses, we observed an overall OR of 5.20 (95% CI 2.08-12.95) for breast cancer developing risk in the Caucasian ethnicity, comparing Gln/Gln type to wild-type Arg/Arg for Arg415Gln polymorphism. In conclusion, this meta-analysis suggests that XPG Asp1104His polymorphism is not associated with increased breast cancer risk, and XPF Arg415Gln may be a low-penetrant risk factor in the Caucasian ethnicity for developing breast cancer. © 2011 Springer Science+Business Media, LLC.

Liu Z.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

Adefovir dipivoxil (ADV) is an acyclic nucleotide phosphate analogue, currently used for anti-HBV therapy. A few cases of hypophosphatemia related to ADV were reviewed. We report two cases of chronic hepatitis B patients with the chief complaints of chest pain due to hypophosphatemia associated with ADV treatment.

Hu X.Q.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To isolate and identify the polysaccharides of Ginkgo biloba and determine their antioxidant activities in vitro. Used hot extraction and alcohol precipitation to get the crude polysaccharides, removed protein with Sevag method, further purify by column chromatography packed with DEAE-52 and DEAE-Sepharose Fast Flow. The homogeneity and molecular weights were evaluated, configuration and monosaccharide composition were measured by IR and high performance ion chromatography analysis, the scavenging activities of GBPB-S on hydroxyl radical and DPPH were measured. The molecular weights of GBPB-W and GBPB-S were 26 300 and 19 100, and both of them were composed of rhamnose, arabinose, galactose, glucose and mannose with the ratios of (3.48: 8.47:3.73: 1.76: 1) and (5.34: 5.37: 5.27: 1:1.68). GBPB-S had certain scavenging effect on hydroxyl radical and DPPH. The polysaccharide isolated from Ginkgo biloba has a direct antioxidant activities in vitro.

Bi W.,Sun Yat Sen University | Zhu L.,Jinan University | Wang C.,Sun Yat Sen University | Liang Y.,Sun Yat Sen University | And 3 more authors.
Brain Research | Year: 2011

Microglial activation plays an important role in the pathophysiology of neurodegenerative diseases, and suppression of microglial activation prevents the progression of neurodegeneration. Rifampicin, a bacteriocidal antibiotic, induces immunosuppression. We hypothesized that rifampicin might be neuroprotective by inhibiting the production of pro-inflammatory mediators, thereby suppressing microglial activation. In the present study, we examined the effects of rifampicin on the production of lipopolysaccharide (LPS)-induced pro-inflammatory mediators and their signaling pathways in BV2 microglia. We also assessed the neuroprotective effects of rifampicin using a co-culture of microglia and neurons. Our results showed that rifampicin inhibited the LPS-stimulated expression of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interleukin-1β, as well as the production of nitric oxide and prostaglandin E 2. Moreover, rifampicin suppressed LPS-induced nuclear factor-kappa B activation by blocking the degradation of the inhibitor of the nuclear transcription factor NF-kappa B. Rifampicin inhibited the phosphorylation of mitogen activated protein kinases, although protein kinase B was not inhibited. Preincubation of microglia with rifampicin reduced neurotoxicity and improved neuron survival in a microglia-neuronal co-culture system. Taken together, these findings suggest that rifampicin, with its anti-inflammatory properties, might be a novel treatment for neurodegenerative diseases. © 2011 Elsevier B.V. All rights reserved.

Duan R.,Chinese University of Hong Kong | Liu S.,Jinan University | Xu J.,Nanjing University of Aeronautics and Astronautics
Archive for Rational Mechanics and Analysis | Year: 2016

The unique global strong solution in the Chemin–Lerner type space to the Cauchy problem on the Boltzmann equation for hard potentials is constructed in a perturbation framework. Such a solution space is of critical regularity with respect to the spatial variable, and it can capture the intrinsic properties of the Boltzmann equation. For the proof of global well-posedness, we develop some new estimates on the nonlinear collision term through the Littlewood–Paley theory. © 2015, Springer-Verlag Berlin Heidelberg.

Lan Y.,Jinan University | Qin J.,University of Science and Technology Beijing | Feng S.,Beijing Normal University
Physics Letters, Section A: General, Atomic and Solid State Physics | Year: 2013

The interplay between the superconducting gap and normal-state pseudogap in the bilayer cuprate superconductors is studied based on the kinetic energy driven superconducting mechanism. It is shown that the charge carrier interaction directly from the interlayer coherent hopping in the kinetic energy by exchanging spin excitations does not provide the contribution to the normal-state pseudogap in the particle-hole channel and superconducting gap in the particle-particle channel, while only the charge carrier interaction directly from the intralayer hopping in the kinetic energy by exchanging spin excitations induces the normal-state pseudogap in the particle-hole channel and superconducting gap in the particle-particle channel, and then the two-gap behavior is a universal feature for the single layer and bilayer cuprate superconductors. © 2013 Elsevier B.V. All rights reserved.

Liu N.,South China University of Technology | Wang Y.,Jinan University | Zhao Q.,South China University of Technology | Cui C.,South China University of Technology | And 2 more authors.
Food Chemistry | Year: 2012

Immobilisation of lecitase® ultra and production of diacylglycerols (DAG) through glycerolysis of soybean oil catalysed by immobilised lecitase® ultra (IM-LU) were investigated in this study. This enzyme was immobilised by macroporous resin adsorption, and IM-LU with an enzyme activity of 1682 U/g, achieved under selected conditions. The results showed that the solvent-free system was preferred for the IM-LU-catalysed glycerolysis reaction as compared with the organic solvent systems. In a solvent-free system, with a reaction of 12 h under the optimum conditions, an upper lipid layer of the reaction mixture with 53.7 wt.% DAG content was obtained. IM-LU exhibited a remarkable reusability in recycling experiments by reusing the recovered IM-LU for 28 consecutive batches. Furthermore, IM-LU was observed to have sn-1, 3-specificity towards triacylglycerols in the glycerolysis reaction and a tendency to enrich saturated fatty acids in DAG with respect to the original oil. © 2012 Elsevier Ltd. All rights reserved.

The study on environmental attitude and behavior intention of tourist is a hot topic in the environmental protection research. Taking Dongzhou islet of Hengyang, in Hunan Province of China as an example, the paper researched the environmental attitude, behavior intention of tourists with environment resource in river islet and their relationship. The study collected the data by questionnaire and analyzed the data by SPSS software. The result shows that the environmental attitude of tourist with environment resource in river islet can be categorized into four dimensions, namely environmental morality, environmental knowledge, environmental responsibility and environmental sentiment. The dimensions of environmental morality, environmental knowledge and environmental sentiment have the positive relations with the environmental behavior intention. © (2012) Trans Tech Publications, Switzerland.

Objectives: To compare the influence of posterior lumbar interbody fusion (PLIF) and transforaminal lumbar interbody fusion (TLIF) on adjacent segment degeneration. Methods: The study was carried out in the Traumatology and Orthopedics Laboratory, Department of Traditional Chinese Medicine, Medical School, Jinan University, Guangzhou, China, between December 2013 and November 2014. A normal, healthy finite element model of L3-5 was developed, a PLIF and a TLIF model were modified from the normal model, and interbody fusions were performed in the L4-5 segment. An 800 N compressive loading plus 10 Nm moments simulating flexion, extension, lateral bending, and axial rotation were imposed on the L3 superior endplate. Intradiscal pressure and intersegmental rotation in L3-4 were investigated. Results: The values of intradiscal pressure and intersegmental rotation in the PLIF or TLIF model were higher than those in the normal, healthy model, but the values in the TLIF model were relatively lower than those in the PLIF model in all directions. Conclusion: Posterior lumbar interbody fusion has more adverse influence on the superior adjacent segment than TLIF. © 2015, Saudi Med J. All right reserved.

Chen Y.,Sun Yat Sen University | Qiao L.,Sun Yat Sen University | Yu B.,Sun Yat Sen University | Li G.,Sun Yat Sen University | And 3 more authors.
Chemical Communications | Year: 2013

An AIPE-active iridium(iii) complex was found to possess high specificity for mitochondria, superior photostability, low cytotoxicity, and high resistance to the loss of mitochondrial membrane potential. Thus, this complex can be used for mitochondrial imaging and tracking in living cells. © 2013 The Royal Society of Chemistry.

Sun Q.,South China Normal University | Chen T.,South China Normal University | Wang X.,Jinan University | Wei X.,South China Normal University | Wei X.,Fudan University
Journal of Cellular Physiology | Year: 2010

Our recent studies have shown that high concentration of taxol induced a caspase-independent paraptosis-like cell death and cytoplasmic vacuolization derived predominantly from endoplasmic reticulum (ER) swelling in human lung carcinoma cell lines (ASTC-a-1). In this report, we further explored the relationship between taxol-induced cell death and vacuolization, and the roles of protein synthesis, mitogen-activated protein kinase kinases (MEK), c-jun N-terminal kinase (JNK) and P38 in taxol-induced paraptosis. Enhanced green fluorescent protein (EGFP) was used to probe the cell morphological change, while ER-targeted red fluorescent protein (er-RFP) was used to probe ER spatial distribution. Real-time monitoring of the ER swelling dynamics during the formation of vacuolization inside single living cells co-expressing EGFP and er-RFP further demonstrated that taxol-induced cytoplasmic vacuolization was from the ER restructuring due to fusion and swelling. PI staining showed that taxol-induced vacuolization was not necrosis. These results further demonstrated that the taxol-induced cell death was neither apoptosis nor necrosis, and fitted the criteria of paraptosis characterized by cytoplasmic vacuolization, caspase-independence, lack of apoptotic morphology and insensitivity to broad caspase inhibitor. Our data further indicated that taxol-induced paraptosis required neither protein synthesis nor the participation of MEK, JNK, and P38, which was different from the insulin-like growth factor I receptor (IGFIR)-induced paraptosis. These results suggest that high concentration of taxol activates an alternative paraptotic cell death pathway. © 2009 Wiley-Liss, Inc.

Using the Nevanlinna theory of the value distribution of meromorphic functions and theory of differential algebra, we investigate the problem of the forms of meromorphic solutions of some specific systems of generalized higher order algebraic differential equations with exponential coefficients and obtain some results. © 2011 Wuhan Institute of Physics and Mathematics.

Huang X.,Jinan University | Barringer S.A.,Ohio State University
LWT - Food Science and Technology | Year: 2016

To investigate the kinetics of furan formation during pasteurization in soy sauce, the furan concentration in five samples was determined with Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS) after hot water bath heating at 30, 50, 70, or 80 °C for 10 min to 7.25 h, respectively. The furan concentrations in soy sauce were 12-215 ng/mL. Furan formation in soy sauce is a zero-order reaction and the reaction rate constants were 3.71 × 10-9 mol/L.h for Pearl River Bridge brand (PRBB) soy sauce and 6.99 × 10-9 mol/Lh for Koon Chun brand (KCSB) at 80 °C respectively. In addition, by the Arrhenius equation and active complex theory, the apparent activation energy (Ea), enthalpy (δH≠θ) and entropy (δS≠θ) were summarized as the equations, lnk = -77237/RT + 6.61 and lnk/T = -74522/RT - 0.18 for PRBB, and lnk = -72018/RT+5.35 and lnk/T = -63301/RT + 0.08 for KCSB, where k is the rate constant, T is the absolute temperature and R is the universal gas constant. Ea, δH≠θ and δS≠θ for PRBB are 77.237 kJ/mol, 74.522 kJ/mol, and -199.01 J/K·mol. Ea, δH≠θ and δS≠θ for KCSB are 72.018 kJ/mol, 63.301 kJ/mol, and -209.72 J/K·mol. These results can be used to calculate or predict furan formation in soy sauce during pasteurization. © 2015.

Ke Y.,Jinan University | Ke Y.,South China University of Technology | Wang Y.J.,South China University of Technology | Ren L.,South China University of Technology | And 2 more authors.
Acta Biomaterialia | Year: 2010

An ideal scaffold provides an interface for cell adhesion and maintains enough biomechanical support during tissue regeneration. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds with pore sizes ranging from 100 to 500 μm and porosity ∼90% were prepared by the particulate-leaching method, and then modified by the introduction of polyacrylamide (PAM) on the inner surface of scaffolds using in situ UV polymerization, with the aim of enhancing the biological and mechanical properties of the PHBV scaffolds. The modified PHBV scaffolds had interconnected pores with porosity of 75.4-78.6% and pore sizes at peak volume from 20 to 50 μm. The compressive load and modulus were up to 62.45 N and 1.06 MPa, respectively. The water swelling percentage (WSP) of the modified PHBV scaffolds increased notably compared with that of the PHBV scaffolds, with the maximum WSP at 537%. Sheep bone mesenchymal stem cells (BMSC) were cultured on the PHBV and modified PHBV. The hydrophilic PAM chains did not influence BMSC viability or proliferation index, but the initial cell adhesion at 1 h of culture was enhanced significantly. Framing PHBV scaffold along with gel-like PAM chains inside is a novel model of inner surface modification for PHBV scaffolds, which shows potential in tissue engineering applications. © 2009 Acta Materialia Inc.

Gao Q.,Max Planck Institute of Colloids and Interfaces | Gao Q.,Jinan University | Giordano C.,Max Planck Institute of Colloids and Interfaces | Antonietti M.,Max Planck Institute of Colloids and Interfaces
Angewandte Chemie - International Edition | Year: 2012

Learning from nature: The development of an MoS2/Ta 3N5 nanocomposite as a catalyst for selective aerobic oxidation by O2 activation was inspired by the nitrogenase enzymes in nature. The superior performance of this biomimetic catalyst, which shows potential for the selective oxidation of multifunctional substrates (see picture), results from the integration of Ta3N5 and MoS2 at the nanoscale and the synergistic enhancement of their activity. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Liu H.,Jinan University
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2012

To introduce the related issues in the clinical translational application of adipose-derived stem cells (ASCs). The latest papers were extensively reviewed, concerning the issues of ASCs production, management, transportation, use, and safety during clinical application. ASCs, as a new member of adult stem cells family, bring to wide application prospect in the field of regenerative medicine. Over 40 clinical trials using ASCs conducted in 15 countries have been registered on the website (http://www.clinicaltrials.gov) of the National Institutes of Health (NIH), suggesting that ASCs represents a promising approach to future cell-based therapies. In the clinical translational application, the related issues included the quality control standard that management and production should follow, the prevention measures of pathogenic microorganism pollution, the requirements of enzymes and related reagent in separation process, possible effect of donor site, age, and sex in sampling, low temperature storage, product transportation, and safety. ASCs have the advantage of clinical translational application, much attention should be paid to these issues in clinical application to accelerate the clinical translation process.

Fu S.,South China University of Technology | Yang H.,South China University of Technology | Li G.,Jinan University | Deng Y.,South China University of Technology | And 2 more authors.
Organic Letters | Year: 2015

The first Cu(II)-catalyzed highly enantioselective intramolecular cyclization of N-alkenylureas was developed for the concise assembly of chiral vicinal diamino bicyclic heterocycles. Facile removal of carbonyl group of the carbamido moiety allowed for ready access to enantioenriched cyclic vicinal diamines. © 2015 American Chemical Society.

Jia S.,Jinan University | Wildner H.,University of Zurich | Birchmeier C.,Max Delbruck Center for Molecular Medicine
Developmental Biology | Year: 2015

Epithelial progenitor cells of the lung generate all cell types of the mature airway epithelium, among them the neuroendocrine cells. The balance between formation of pulmonary neuroendocrine and non-neuroendocrine cells is controlled by Notch signaling. The Notch target gene. Hes1 is expressed by non-neuroendocrine and absent in neuroendocrine cells. The transcription factor. Ascl1 is expressed in a complementary pattern and provides key regulatory information that specifies the neuroendocrine cell fate. The molecular events that occur after the induction of the neuroendocrine differentiation program have received little attention. Here we show that. Insm1 is expressed in pulmonary neuroendocrine cells, and that. Insm1 expression is not initiated in the lung of. Ascl1 mutant mice. We use mouse genetics to show that pulmonary neuroendocrine cells depend on. Insm1 for their differentiation. Mutation of. Insm1 blocks terminal differentiation, upregulates Hes1 protein in neuroendocrine cells and interferes with maintenance of. Ascl1 expression. We show that Insm1 binds to the. Hes1 promoter and represses. Hes1, and we propose that the Insm1-dependent. Hes1 repression is required for neuroendocrine development. Our work demonstrates that Insm1 is a key factor regulating differentiation of pulmonary neuroendocrine cells. © 2015 The Authors.

Niu Y.,Shantou University | Chen K.C.,Shantou University | He T.,Shantou University | Yu W.,Shantou University | And 2 more authors.
Biomaterials | Year: 2014

Nerve guide scaffolds from block polyurethanes without any additional growth factors or protein were prepared using a particle leaching method. The scaffolds of block polyurethanes (abbreviated as PUCL. ran-EG) based on poly(e{open}-caprolactone) (PCL-diol) and poly(ethylene glycol) (PEG) possess highly surface-area porous for cell attachment, and can provide biochemical and topographic cues to enhance tissue regeneration. The nerve guide scaffolds have pore size 1-5 μm and porosity 88%. Mechanical tests showed that the polyurethane nerve guide scaffolds have maximum loads of 4.98 ± 0.35 N and maximum stresses of 6.372 ± 0.5 MPa. The histocompatibility efficacy of these nerve guide scaffolds was tested in a rat model for peripheral nerve injury treatment. Four types of guides including PUCL. ran-EG scaffolds, autograft, PCL scaffolds and silicone tubes were compared in the rat model. After 14 weeks, bridging of a 10 mm defect gap by the regenerated nerve was observed in all rats. The nerve regeneration was systematically characterized by sciatic function index (SFI), histological assessment including HE staining, immunohistochemistry, ammonia silver staining, Masson's trichrome staining and TEM observation. Results revealed that polyurethane nerve guide scaffolds exhibit much better regeneration behavior than PCL, silicone tube groups and comparable to autograft. Electrophysiological recovery was also seen in 36%, 76%, and 87% of rats in the PCL, PUCL. ran-EG, and autograft groups respectively, whilst 29.8% was observed in the silicone tube groups. Biodegradation in vitro and in vivo show proper degradation of the PUCL. ran-EG nerve guide scaffolds. This study has demonstrated that without further modification, plain PUCL. ran-EG nerve guide scaffolds can help peripheral nerve regeneration excellently. © 2014 Elsevier Ltd.

Ge R.,Sun Yat Sen University | Sun X.,Jinan University
BioMetals | Year: 2012

Helicobacter pylori infections are closely associated with peptic ulcers, gastric malignancy and iron deficiency anemia. Iron is essential for almost all living organisms and the investigation of iron uptake and trafficking system is thus important to understand the pathological roles of H. pylori. Up to now, the iron trafficking system of H. pylori is not yet fully clear and merits further efforts in this regards. The available information about iron uptake and regulation has been discussed in this concise review, such as FeoB in ferrous transportation, FrpB2 in hemoglobin uptake, HugZ in heme processing, virulence factors (VacA and CagA) in transferrin utilization, Pfr and NapA in iron storage and Fur in iron regulation. The identified iron trafficking system will help us to understand the pathological roles of H. pylori in the various gastric diseases and iron deficiency anemia and stimulates further development of effective anti-bacterial drugs. © Springer Science+Business Media, LLC. 2011.

Wang H.,Sun Yat Sen University | Wang H.-S.,Sun Yat Sen University | Zhou B.-H.,Sun Yat Sen University | Li C.-L.,Sun Yat Sen University | And 6 more authors.
PLoS ONE | Year: 2013

Chronic inflammation-promoted metastasis has been considered as a major challenge in cancer therapy. Pro-inflammatory cytokine TNFα can induce cancer invasion and metastasis associated with epithelial-mesenchymal transition (EMT). However, the underlying mechanisms are not entirely clear. In this study, we showed that TNFα induces EMT in human HCT116 cells and thereby promotes colorectal cancer (CRC) invasion and metastasis. TNFα-induced EMT was characterized by acquiring mesenchymal spindle-like morphology and increasing the expression of N-cadherin and fibronectin with a concomitant decrease of E-cadherin and Zona occludin-1(ZO-1). TNFα treatment also increased the expression of transcription factor Snail, but not Slug, ZEB1 and Twist. Overexpression of Snail induced a switch from E-cadherin to N-cadherin expression in HCT116 cells, which is a characteristic of EMT. Conversely, knockdown of Snail significantly attenuated TNFα-induced EMT in HCT116 cells, suggesting that Snail plays a crucial role in TNFα-induced EMT. Interestingly, exposure to TNFα rapidly increased Snail protein expression and Snail nuclear localization but not mRNA level upregulation. Finally, we demonstrated that TNFα elevated Snail stability by activating AKT pathway and subsequently repressing GSK-3β activity and decreasing the association of Snail with GSK-3β. Knockdown of GSK-3β further verified our finding. Taken together, these results revealed that AKT/GSK-3β-mediated stabilization of Snail is required for TNFα-induced EMT in CRC cells. Our study provides a better understanding of inflammation-induced CRC metastasis. © 2013 Wang et al.

But P.P.-H.,Chinese University of Hong Kong | Jiang R.-W.,Chinese University of Hong Kong | Jiang R.-W.,Jinan University | Shaw P.-C.,Chinese University of Hong Kong
Journal of Ethnopharmacology | Year: 2013

Ethnopharmacological relevance: Red edible bird's nests are regarded as of higher beneficial value for health and hence fetch a higher price than the white ones. Their red colour remains a myth. Aim of the study: To determine if white edible bird's nests can turn red by vapours generated from sodium nitrite in acidic conditions and by vapours from 'bird soil'. Materials and methods: White edible bird's nests were exposed to vapours from sodium nitrite dissolved in 2% HCl or from 'bird soil' in hot and humid conditions. Conclusions: Vapours from sodium nitrite dissolved in 2% HCl or from 'bird soil' containing guano droppings from swiftlet houses were able to turn white edible bird's nests red. The reddening agent in 'bird soil' was water-soluble and heat-stable. The red colour of edible bird's nests is likely caused by the environmental factors in cave interiors and swiftlet houses. © 2012 Elsevier Ireland Ltd.

Gu B.,Jinan University
International Review of Hydrobiology | Year: 2012

Nitrogen stable isotopes (δ 15N) of dissolved inorganic nitrogen (DIN = NH 4 + and NO 3 -), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON) were measured in Smith Lake, Alaska to assess their usefulness as proxies for the biological nitrogen cycling processes, nutrient concentration, and lake productivity. Large seasonal variations in δ 15NH 4 +, δ 15NO 3 - and δ 15N PON occurred in response to different processes of nitrogen transformation that dominated a specific time period of the annual production cycle. In spring, 15N depletion in all three pools was closely related to the occurrences of a N 2-fixing cyanobacterial bloom (Anabaena flos-aquae). In summer, δ 15N PON increased as phytoplankton community shifted to use NH 4 + and decreased as a brief N 2-fixing bloom (Aphanizomenon flos-aquae) occurred in August. In early and mid-winter, microbial nitrogen processes were dominated by nitrification that resulted in the largest isotope fractionation between NO 3 - and NH 4 + in the annual cycle. This was followed by denitrification that led to the highest 15N enrichment in NO 3 -. A peak of NH 4 + assimilation by phytoplankton along with the elevated δ 15N PON and Chl a concentration occurred just before the ice break due to increased light penetration. The δ 15N DON displayed little temporal and spatial variations. This suggests that the DON pool was not altered by biological transformations of nitrogen as the results of its large size and possibly refractory nature. There was a positive correlation between Chl a concentration and δ 15N PON, and a negative correlation between NH 4 + and δ 15N PON, suggesting that δ 15N PON is a useful proxy for nitrogen productivity and ammonium concentration. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Wu W.,South China University of Technology | Wang C.,Jinan University | Zheng J.,South China University of Technology
LWT - Food Science and Technology | Year: 2012

Molecular distillation for deacidification of crude low-calorie cocoa butter was optimized. Processing parameters of molecular distillation including evaporator temperature, feed flow rate and pressure were investigated on the quality indicators (acid value and color) of refined low-calorie cocoa butter by single factor experiments. Optimum operating parameters were obtained by response surface methodology. Evaporator temperature, feed flow rate and their interaction term showed significant effects on the responses (deacidification rate and Lovibond color), but pressure was not significant. The optimum parameters for production of low-calorie cocoa butter with low acidity and light color were as follows: evaporator temperature 185.0. °C, feed flow rate 2.3. mL/min and pressure of 1.0. Pa. The predicted results of deacidification rate and yellow and red values (95.25%, 5.5 and 0.7) were close to the experimental values (95.48±0.48%, 5.5±0.7 and 0.6±0.1). © 2011 Elsevier Ltd.

Liu D.,Jinan University
Journal of Ambient Intelligence and Humanized Computing | Year: 2014

Real-time online interactive application (ROIA) is an emerging large scale distributed application in recent years. To satisfy the high demands on performance, ROIA needs a highly robust and efficient architecture to cope with the huge concurrent users. Previous works are almost based on the Client/Server or Peer to Peer mode, and their scalability and resource utilization are relatively low. So we try to take advantage of the cloud computing technologies to achieve higher scalability and resource utilization. However, the node failure is no longer considered to be an accidental event, but a normal one in the cloud computing. Therefore, there are higher requirements for fault tolerance in ROIA. In this paper, we propose a new fault-tolerant architecture for ROIA in cloud, which is based on cell overlapping technique. This new architecture provides redundancy to enhance the robustness and the scalability of ROIA. We focus on analyzing three merits of new architecture: seamless migration across zones, server crash protection and dynamic load balance. This paper sheds a little light to the related further research on ROIA. © 2014 Springer-Verlag Berlin Heidelberg

Deng J.,China National Analytical Center Guangzhou | Fan C.,Jinan University | Yang Y.,China National Analytical Center Guangzhou
Journal of Pharmaceutical and Biomedical Analysis | Year: 2011

Deng's herbal tea (DHT), a famous traditional Chinese herbal tea consisting of six traditional Chinese medicines (Honeysuckle, Chrysanthemum, Rhizoma imperatae, Folium mori, dandelion and liquorice), is widely used in China for its health benefits. In this paper, a rapid resolution liquid chromatography coupled with mass spectrometry (RRLC-MS) method was developed for the identification and determination of the major constituents in DHT granules. A good RRLC separation was achieved using an Agilent Poroshell 120 SB-C 18 column and gradient elution (0.5% formic acid in water/acetonitrile) within 30min. Twenty-eight compounds were identified or tentatively characterized based on their exact molecular weights and fragmentation patterns. Fifteen major bioactive constituents of those 28 compounds were chosen as the benchmark substances. Their quantitative analyses were performed by a triple quadrupole tandem mass spectrometer (MS/MS) operating in multiple-reaction monitoring mode, and a full quantitative analysis of the 15 major constituents was performed by our developed RRLC-MS/MS method in only 10min. Of the 16 DHT granule samples tested, the quality of the results was stable, which confirms that the developed method was efficient and robust for the quality control of DHT granules. © 2011 Elsevier B.V.

Han L.,China University of Mining and Technology | Han Z.,Jinan University
ICCMS 2010 - 2010 International Conference on Computer Modeling and Simulation | Year: 2010

Graph coloring problem is a classical NP-hard combinatorial optimization problem. In this paper, a new bi-objective model for the coloring problem is presented. Based on this new model, a bi-objective genetic algorithm is proposed which employs effective crossover and simple mutation operator as the genetic operators. The global convergence of the proposed algorithm to globally optimal set with probability one is proved. Experimental results demonstrate that the proposed algorithm is expected. © 2010 IEEE.

Nie P.-Y.,Jinan University
Economic Computation and Economic Cybernetics Studies and Research | Year: 2010

This paper establishes model with spatial competitions to characterize technology spillover. Based on spatial competitions and technology spillover, we achieve the optimal distance between two firms. We show that industrial clusters are formed if technology spillovers are not prominent. The industrial organization approaches are focused on to rationally explain technology spillovers under spatial competitions.

Xie H.,Jinan University
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2011

The bone marrow mesenchymal stem cells (BMSCs) have the capacity to differentiate into insulin-producing cells (IPCs) in vitro. However, low differentiation efficiency and poor maturity are the main obstacles. To investigate the feasibility of BMSCs differentiation into IPCs in diabetic pancreatic microenvironment of pigs. BMSCs were isolated and purified from the bone marrow of a 4-week-old male pig. Fifteen female pigs (aged 8 to 10 weeks, weighing 8 to 10 kg) were randomly divided into 3 groups: normal control group (group A, n=5), diabetic control group (group B, n=5), and BMSCs transplanted group (group C, n=5). The pigs of groups B and C were treated by auris vein injections of streptozocin and alloxan for 3 days to induce diabetes mellitus (DM) model, whose blood glucose level 2 days all greater than 17 mmol/L was successful DM model. A total of 1.1 mL of the 3rd passage BMSCs labeled with enhanced green fluorescent protein (EGFP), with cell density of 5 x 10(7)/ mL, were injected into subcapsular pancreas of group C at multiple points, normal saline at the same dosage into those of groups A and B. After 30 days of monitoring blood glucose, the histological analysis of islet number and size were done; the immunofluorescence staining was used to detect the protein expression of insulin in the new-formed islets. The EGFP+ cells were collected from the sections using laser-capture microdissection; RT-PCR was used to detect insulin mRNA and pancreatic and duodenal homeobox factor 1 (PDX1) mRNA expressions from EGFP+ cells, and the insulin and sex determining region of the Y chromosome (SRY) genes were detected by fluorescence in situ hybridization (FISH). The blood glucose level decreased significantly in group C when compared with that in group B from 18 days and gradually decreased with time (P < 0.05). The histological observation showed that the number of islets was increased significantly in group C when compared with that in group B (10.9 +/- 2.2 vs. 4.6 +/- 1.4, P < 0.05), and there was no significant difference when compared with that in group A (10.9 +/- 2.2 vs.12.6 +/- 2.6, P > 0.05). The size of new-formed islets in group C was significantly smaller than that in group A [(47.2 +/- 19.6) microm vs. (119.6 +/- 27.7) microm, P < 0.05]. The immunofluorescence staining showed that new-formed islets of group C expressed insulin protein. RT-PCR showed that the microdissected EGFP+ cells of group C expressed insulin mRNA and PDX-1 mRNA. FISH showed that the new-formed islet cells of group C contained SRY gene in Y chromosome and insulin double positive cells. BMSCs can differentiate into IPCs in diabetic pancreatic microenvironment of pigs.

Xue X.Y.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To study the neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons. The newborn rat cerebral cortex neurons were cultured in vitro. The convert phase microscope was used to count the survival neurons with neurites and measure the average length of neurites. MTT and LDH assay were carried out to investigate the effect of PCA on the neuronal viability. Compared with control group, different concentration of PCA could increase the number of survival neurons with neurites and the average length of neurites. MTT and LDH assay showed that PCA promoted neuron survival in a dose-dependent manner. PCA can enhance the survival of rat cortical neurons with neurites and promote the neurite outgrowth of rat cortical neurons.

Dong M.,Shenzhen University | Jiang X.,Jinan University | Jiang X.,University of Chicago | Yan B.P.,Chinese University of Hong Kong
Clinical Cardiology | Year: 2013

Background Recent evidence suggests that Rho-kinase (ROCK) plays an important role in the pathogenesis of atherosclerosis and a marker of atherosclerotic burden. Polyvascular disease with concomitant peripheral arterial disease (PAD) and coronary artery disease (CAD) is common and associated with a worse prognosis. The aim of this study was to evaluate ROCK activity as a marker of polyvascular disease. Hypothesis Methods We retrospectively analyzed patients undergoing coronary angiography at our institution between February 2009 and May 2009. Patients with only CAD (n = 40) defined by coronary artery stenosis of ≥50% by angiography, only PAD (n = 40) defined by an ankle brachial index (ABI) <0.9, and combined CAD/PAD (n = 40) were matched by age and sex to control patients (n = 40) without CAD or PAD. ROCK activity was determined by phosphorylation of the myosin binding subunit in leukocytes and then compared between each group. Multivariate analysis was used to determine independent predictors of polyvascular disease. Discriminative ability of elevated ROCK activity was assessed using receiver operator characteristics (ROC) curves. Results Patients (age 68 ± 12 years, 79% male) with CAD, PAD, and CAD/PAD had a mean ABI of 1.08, 0.62, and 0.65, respectively, compared to 1.08 in the control group. There was an incremental increase in ROCK activity in patients with CAD (4.61 ± 2.11), PAD (4.27 ± 1.39), and CAD/PAD (5.96 ± 1.94) compared to control (2.40 ± 0.43) (all P < 0.05). ROCK activity (odds ratio: 4.53, 95% confidence interval: 1.26-6.30) was an independent predictor of polyvascular disease. The ROCK cutoff value of 4.85 had a sensitivity of 72.7% and a specificity of 65.7%, with an area under ROC curve of 0.71 for polyvascular disease. Conclusions Patients with concomitant peripheral and coronary arterial disease are associated with increased Rho-kinase activity. Rho-kinase activity may be a potential marker of atherosclerotic burden for patients with polyvascular disease. © 2013 Wiley Periodicals, Inc.

Zhao S.,Jinan University | Ma X.,Sun Yat Sen University
IEEE Photonics Technology Letters | Year: 2016

Visible light communication (VLC) systems are expected to support a variety of applications, such as common-information broadcasting, real-time multimedia streaming, and large file downloading. Typically, these applications have different delay requirements. Hence, it is desirable to design high-performance coding scheme capable of supporting a wide range of delays but with acceptable hardware complexity. To achieve this, we propose a delay-tunable coding scheme for VLC systems based on block Markov superposition transmission of short non-binary low-density parity-check (NBLDPC) codes. The proposed coding scheme includes the following advantages: 1) it is easily configurable to fulfill different delay requirements while keeping the code rate constant; 2) it requires essentially the same hardware modules to implement the encoder/decoder as the involved short NBLDPC code; and 3) it can have a larger coding gain if a longer delay is tolerated. Numerical results are presented to show the expected tradeoff between delay and energy in a VLC system. © 1989-2012 IEEE.

Luo J.M.,Jinan University
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] | Year: 2012

A simple, rapid and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was established to detect norovirus genotype GII. The method employed a set of six specially designed primers that recognized eight distinct sequences of RNA-dependant RNA polymerase and capsid protein gene for amplification of nucleic acid under isothermal conditions at 65 degrees C for 60 minutes. The amplification process of RT-LAMP was monitored by the addition of HNB (Hydroxy naphthol blue) dye prior to amplification. A positive reaction was indicated by a color change from violet to sky blue and confirmed by agarose electrophoresis. The specificity of the RT-LAMP was validated by detecting several different diarrhea viruses including norovirus genotype GII. The sensitivity was determined by serial dilutions of RNA molecules from in vitro transcription of norovirus genotype GII in parallel with conventional RT-PCR detection. The assay was further evaluated with 93 clinical specimens of diarrhea patients. The results showed that the sensitivity of RT-LAMP was 1 000 copies/microL with a high specificity and the relative sensitivity was at the same level as that of conventional RT-PCR. Positive rate of RT-LAMP in analysis of clinical specimens was approximately the same as that of conventional RT-PCR as well. This colorimetric RT-LAMP assay was potential for rapid detection of norovirus genotype GII on spot due to the observation of visual result with high specificity and sensitivity, time-saving and cost benefit.

Jia H.,Jinan University | Jia H.,University of Southern California | Guo Y.,University of Southern California | Zhao W.,KingMed Diagnostics | Wang K.,University of Southern California
Scientific Reports | Year: 2014

Long-range PCR remains a flexible, fast, efficient and cost-effective choice for sequencing candidate genomic regions in a small number of samples, especially when combined with next-generation sequencing (NGS) platforms. Several long-range DNA polymerases are advertised as being able to amplify up to 15...kb or longer genomic DNA. However, their real-world performance characteristics and their suitability for NGS remain unclear. We evaluated six long-range DNA polymerases (Invitrogen SequalPrep, Invitrogen AccuPrime, TaKaRa PrimeSTAR GXL, TaKaRa LA Taq Hot Start, KAPA Long Range HotStart and QIAGEN LongRange PCR Polymerase) to amplify three amplicons, with sizes of 12.9...kb, 9.7...kb, and 5.8...kb, respectively. Subsequently, we used the PrimeSTAR enzyme to amplify entire BRCA1 (83.2...kb) and BRCA2 (84.2...kb) genes from nine subjects and sequenced them on an Illumina MiSeq sequencer. We found that the TaKaRa PrimeSTAR GXL DNA polymerase can amplify almost all amplicons with different sizes and Tm values under identical PCR conditions. Other enzymes require alteration of PCR conditions to obtain optimal performance. From the MiSeq run, we identified multiple intronic and exonic single-nucleotide variations (SNVs), including one mutation (c.5946delT in BRCA2) in a positive control. Our study provided useful results for sequencing research focused on large genomic regions.

Tan C.,Sun Yat Sen University | Hu S.,Guangdong University of Technology | Liu J.,Sun Yat Sen University | Liu J.,Jinan University | Ji L.,Sun Yat Sen University
European Journal of Medicinal Chemistry | Year: 2011

Two new ruthenium complexes, trans,cis,cis-[RuCl2(DMSO) 2(H2biim)] (1) and mer-[RuCl3(DMSO)(H 2biim)] (2) (DMSO = dimethyl sulfoxide and H2biim = 2,2′-biimidazole), have been synthesized and fully characterized by single-crystal X-ray analysis. The less stable complex 2 is more cytotoxic against the four human cancer cell lines tested than 1. Further studies show that 1 and 2 exhibit cell growth inhibition by triggering G0/G1 cell cycle arrest and mitochondria-mediated apoptosis. Additionally, complex 2 exerts potent inhibitory effects on the adhesion and migration of human cancer cells comparable to that of NAMI-A ([ImH][trans-[RuCl4(Im)(DMSO-S)], Im = imidazole). Target validation studies show that cyclin-dependent kinases (CDKs), other than DNA, are more likely to be targets of 1 and 2. © 2011 Elsevier Masson SAS. All rights reserved.

Luo Y.,Jinan University
Guangdian Gongcheng/Opto-Electronic Engineering | Year: 2011

In optical alignment, because quantizing and compensating of the coupling relation keeps a problem, the coordinates coupling is usually ignored, resulting in adjusting error in accurate optical adjusting. Through modeling and simulation, the coupling relation of each adjusting freedom of a multidimensional precision optical adjusting frame is analyzed in detail. Through decomposed transformation of each adjusting freedom, the coupling relation between each adjusting freedom is compensated and corrected. Test result shows that: by using this method, the error between expected optical adjusting value and actual mechanical adjusting value can be reduced effectively, and more accurate optical adjusting is achieved.

Wang X.,Chinese University of Hong Kong | Xie W.,Chinese University of Hong Kong | Xie W.,Jinan University | Du J.,Chinese University of Hong Kong | And 4 more authors.
Advanced Materials | Year: 2012

Graphene field-effect transistors (GFETs) with different metal electrodes are fabricated to explore the contact characteristics. The contact resistance and the spatial potential distribution along the graphene/metal interface are investigated. The low-doped graphene/metal contact can be reversibly switched between "ohmic" and "space-charge region limited" states. The observed switching attributes are highly reproducible and stable, which provides a new avenue to produce high-performance graphene memory devices. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Tyurin V.A.,University of Pittsburgh | Balasubramanian K.,University of Pittsburgh | Winnica D.,University of Pittsburgh | Tyurina Y.Y.,University of Pittsburgh | And 7 more authors.
Cell Death and Differentiation | Year: 2014

Diversified anionic phospholipids, phosphatidylserines (PS), externalized to the surface of apoptotic cells are universal phagocytic signals. However, the role of major PS metabolites, such as peroxidized species of PS (PSox) and lyso-PS, in the clearance of apoptotic cells has not been rigorously evaluated. Here, we demonstrate that H 2O2 was equally effective in inducing apoptosis and externalization of PS in naive HL60 cells and in cells enriched with oxidizable polyunsaturated species of PS (supplemented with linoleic acid (LA)). Despite this, the uptake of LA-supplemented cells by RAW264.7 and THP-1 macrophages was more than an order of magnitude more effective than that of naive cells. A similar stimulation of phagocytosis was observed with LA-enriched HL60 cells and Jurkat cells triggered to apoptosis with staurosporine. This was due to the presence of PSox on the surface of apoptotic LA-supplemented cells (but not of naive cells). This enhanced phagocytosis was dependent on activation of the intrinsic apoptotic pathway, as no stimulation of phagocytosis occurred in LA-enriched cells challenged with Fas antibody. Incubation of apoptotic cells with lipoprotein-associated phospholipase A 2 (Lp-PLA 2), a secreted enzyme with high specificity towards PSox, hydrolyzed peroxidized PS species in LA-supplemented cells resulting in the suppression of phagocytosis to the levels observed for naive cells. This suppression of phagocytosis by Lp-PLA 2 was blocked by a selective inhibitor of Lp-PLA 2, SB-435495. Screening of possible receptor candidates revealed the ability of several PS receptors and bridging proteins to recognize both PS and PSox, albeit with diverse selectivity. We conclude that PSox is an effective phagocytic 'eat-me' signal that participates in the engulfment of cells undergoing intrinsic apoptosis. © 2014 Macmillan Publishers Limited All rights reserved.

Lai Y.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

To investigate the effect of dexmedetomidine on oxygenation function in adult patients with balanced anesthesia by propofol-fentanyl under one-lung ventilation (OLV). Twenty-two patients undergoing thoracic operation were randomly divided into the study group and control group, both receiving propofol and fentanyl balanced anesthesia. In the study group, additional infusion of dexmedetomidine (0.3 μg/kg loading dose, 0.3 μg·kg(-1)·h(-1) maintenance dose) was administered, and the patients in the control group received only normal saline. Arterial blood samples were obtained at 4 time points from each patient during anesthesia for blood gas analysis. In the study group, the pH values remained stable, the oxygenation index tended to decline progressively, but the incidence of hypoxemia was low; in the control group, the pH value and oxygenation index both declined progressively with a higher incidence of hypoxemia. Dexmedetomidine can better maintain the oxygenation function of OLV patients in balanced anesthesia by propofol and fentanyl, and its mechanism may be related to the decreased dose of propofol used.

Guan B.-O.,Jinan University | Wang S.-N.,Dalian University of Technology
IEEE Photonics Technology Letters | Year: 2010

A novel fiber-optic current sensor based on a dual-polarization fiber grating laser is proposed and experimentally demonstrated. A permanent magnet exerts transversal force to the dual-polarization fiber grating laser due to the action of a magnetic field produced by an electric current. The transversal force modulates the fiber birefringence and, therefore, the beat frequency between the two polarization modes from the fiber grating laser. The sensor is capable of measuring both dc and ac current, and has advantages of ease of interrogation, absolute frequency encoding, and capability to multiplex a number of sensors on a single fiber. © 2010 IEEE.

Liu X.,Jinan University
2nd International Workshop on Education Technology and Computer Science, ETCS 2010 | Year: 2010

Scenarios are critical for requirement analysis and system design. In this paper, we present a lightweight framework for scenario elicitation from natural language requirements. First, the events are elicited from sentences using event templates. Then the elicited events are associated with event tree to constitute scenarios. © 2010 IEEE.

Chen Y.P.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011

To explore the morbidity of hypertension and prehypertension and analyze the association between body mass index (BMI) and blood pressure in Chinese college students. This epidemiological study involved 490 Chinese college students (aged 15-25 years, mean 18.9∓1.2 years), and their body height, body weight, blood pressure and heart rate were recorded. The BMI was divided into four groups according to Chinese population standard classification. Most students were southern Han Chinese. The incidences of overweight plus obesity and obesity were 12.9% and 4.1%, respectively. The morbidity of hypertension was 0.8% in this student population. The incidences of hypertension and pre-hypertension were higher in high BMI groups than those in low BMI groups. The systolic blood pressures were significantly higher in over-weight and obese groups than in the normal BMI and lean groups. BMI was positively correlated to systolic and diastolic blood pressures in this population. High BMI is a predictor of elevated blood pressure in adolescent students.

Zhang Y.M.,Jinan University
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2013

To observe the arousal effect of electroacupuncture (EA) stimulation of Baihui (GV 20), Shuigou (GV 26), etc. on severe craniocerebral injury patients. A total of 90 cases of severe craniocerebral injury were randomly allocated to routine medication, naloxone and EA groups, with 30 cases in each group. For patients of the routine medication group, mild hypothermia therapy, medicines for dehydration, hormonal therapy, vascular dilation, cerebral nutrition supporting, anti-inflammation, etc. were given. For patients of the naloxone group, intravenous drip of naloxone 0.4 mg/kg in the first 3 days, 0.2 mg/kg for 7 days and 0. 1 mg/kg afterwards. For patients of the EA group, EA (1 Hz/50 Hz) was given for 30 min once daily. All the treatments were conducted once a day for 14 days. The Glasgow Coma Scale (GCS) and Glasgow Outcome Scale (GOS) were used for assessing the therapeutic effect. In comparison with pre-treatment in each one of the routine medication, naloxone and EA groups, GCS scores were all obviously increased in the 3 groups following the treatment, and one month's follow-up (P<0. 05). The GCS scores of both naloxone and EA groups were significantly higher than those of the routine medication group (P<0.05). No significant difference was found between the naloxone group and EA group in GCS scores (P>0. 05). According to the GOS, one month's follow-up showed that of the three 30 cases in the routine medication, naloxone and EA groups, 6, 12 and 14 were improved, 8, 10 and 10 moderate handicap, 8, 3 and 2 severe handicap, 5, 3 and 2 vegetative state, and 3, 2 and 2 dead, with the arousal rates being 46. 66% , 73. 33% and 80. 00%, respectively. The therapeutic effects of both naloxone and EA groups were significantly superior to those of the routine medication group (P<0.05). EA intervention at early stage can promote the recovery of neurological function, accelerate the consciousness from coma and improve the outcomes of patients with severe traumatic brain injury.

Xu X.,CAS South China Botanical Garden | Xu X.,University of Chinese Academy of Sciences | Xie H.,CAS South China Botanical Garden | Wang Y.,Jinan University | Wei X.,CAS South China Botanical Garden
Journal of Agricultural and Food Chemistry | Year: 2010

Two new A-type trimeric proanthocyanidins with two doubly bonded interflavanoid linkages, litchitannin A1 [epicatechin-(2β→O→7, 4β→6)-epicatechin-(2β→O→7,4β→8)-catechin] (1) and litchitannin A2 [epicatechin-(2β→O→7,4β→6)- epicatechin-(2β→O→7,4β→6)-epicatechin] (2), were isolated from lychee (Litchi chinensis Sonn. cv. Heiye) seeds together with aesculitannin A (3), epicatechin-(2β→O→7,4β→8)- epiafzelechin-(4α→8)-epicatechin (4), proanthocyanidin A1 (5), proanthocyanidin A2 (6), proanthocyanidin A6 (7), epicatechin-(7,8-bc)-4β- (4-hydroxyphenyl)-dihydro-2(3H)-pyranone (8), and epicatechin (9). Their structures were elucidated on the basis of spectroscopic and chemical evidence. It is the first time that compounds 1-4, 7, and 8 have been reported in this species. Compounds 1-9 showed more potent antioxidant activity than l-ascorbic acid with ferric reducing antioxidant power (FRAP) values of 3.71-24.18 mmol/g and IC50 values of 5.25-20.07 μM toward DPPH radicals. Moreover, litchitannin A2 (2) was found to exhibit in vitro antiviral activity against coxsackie virus B3 (CVB3) and compounds 3 and 6 displayed antiherpes simplex virus 1 (HSV-1) activity. © 2010 American Chemical Society.

Zhu Y.,Hong Kong University of Science and Technology | Wang H.,Yunnan University of Finance and Economics | Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
International Journal of Plasticity | Year: 2014

One of the main targets in the development of dislocation based continuum crystal plasticity theories is to establish continuum constitutive relations which approximately summarize the underlying discrete dislocation dynamics (DDD). However, rigorously transiting from discrete to continuum in describing the evolution of dislocation system is extremely challenging for complex networks of curved dislocations and their interactions at multiple length scales. To address this difficulty, a coarse-grained disregistry function (CGDF) was proposed to represent the continuous distributions of curved dislocations. In this paper, we present a dislocation based continuum model for crystal plasticity incorporating the Frank-Read sources, which serves as a crucial step towards systematically building a three-dimensional dislocation based continuum plasticity theory. The continuum model is derived accurately from the DDD model, and is validated by comparisons of the results with theoretical predictions and DDD simulations conducted under the same conditions. Furthermore by considering dislocation loop pileups within a rectangular grain, we derive analytical formulas which generalize the traditional Hall-Petch relation into two dimensions without any adjustable parameters. It is shown that the yield stress of a rectangular grain depends not only on the grain size, but also on the grain aspect ratio whose exact form is associated with the harmonic mean of the length and width of the rectangle. The derived formulas of the yield stress are shown in excellent agreement with the results by our continuum model and DDD simulations. © 2014 Elsevier Ltd. All rights reserved.

Zheng H.,Jinan University
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2011

To explore the patients' genotypes and the mutation spectrum of Tyrosinase (TYR) gene and the effects on protein structure and function in oculocutaneous albinism type 1 (OCA1). The polymerase chain reaction (PCR) and sequencing techniques were applied to amplify and analyze the regions of exon, exonintron and promoter of TYR gene of 15 OCA1 probands and some of their parents. The protein structure and function were forecasted and analyzed by bioinformatics software. Sequencing result showed 11 kinds of mutations, including 5 missense mutations (W400L, R299H, E294K, R77Q and K142M), 3 nonsense mutations (R116X, R278X and G295X), 2 insertion mutation (929insC and 232insGGG) and 1 splice site mutation (IVS1-3C > G). The nosogenesis was related to the change of protein structure and function in four pathological mutations. It seemes that W400L is the frequent mutations, which accounted for about 30.0% in Chinese mainland OCA1 alleles. It is doable to make some reasonable interpretation about TYR gene nosogenesis by bioinformatics method.

Lyu C.,Tianjin University | Wu C.,Hong Kong Polytechnic University | Wu C.,Jinan University | Tam H.-Y.,Hong Kong Polytechnic University | And 2 more authors.
Optics Express | Year: 2013

A DBR fiber grating laser acoustic sensor based on polarization beat signal modulation analysis has been demonstrated for directional acoustic signal measurement. The acoustic sensor was fabricated in birefringent erbium-doped fiber, and the influences of external-acoustic pressure on fiber grating laser sensor were analyzed, considering the effect of relative orientation of the acoustic wave on the degrees of birefringence modulation. In experiment, the birefringence in sensing fiber was modulated by ultrasonic pressure. Agreement between theoretical and experimental results was obtained for ultrasound wave propagating from different directions (0-360 degrees in 15 degrees intervals) corresponding to a nonlinearly change in beat frequency modulation rates. The results demonstrate that the DBR fiber grating laser acoustic sensor has an orientation recognizable ability, offering a potential for acoustic vector signal detection. © 2013 Optical Society of America.

Chen A.,Jinan University | Groenewold N.,University of Western Australia
Growth and Change | Year: 2011

Despite high economic growth over the past 30 years, China's substantial and persistent regional disparities have been the subject of continuing concern to policy makers, as well as the target of a wide variety of policies. An important issue in the policy debate about whether and how best to attack these disparities is whether measures designed to improve regional equality come at a cost to national development, i.e., whether there is a trade-off between the level of national output and the equality of its distribution across the regions. There is little analysis of this issue in the literature. We help fill this gap by setting up a two-region model designed to capture some of the salient features of the Chinese economy. We subject this model to a number of policy shocks and assess the effects on regional disparities in per capita output, on the one hand, and on aggregate output on the other to investigate the trade-off. We also consider income and welfare as alternatives to output. We find, first, that disparities in per capita output, income, and welfare may move in different directions so that it is important to specify which disparity is being targeted. Second, since both disparities and aggregate outcomes are endogenous, how they move together depends on the nature of the shock driving the model. Thus, some policies designed to reduce disparities face a trade-off and others do not. Only a reduction in internal migration restrictions unambiguously reduces all three disparity measures and increases aggregate output, income, and welfare. All other policies considered face a trade-off in at least one dimension. Third, whether there is a trade-off depends also on the time horizon-some policies face a trade-off in the short run and not in the long run and vice versa. © 2011 Wiley Periodicals, Inc.

Wang Y.,CAS Guangzhou Institute of Geochemistry | Wang Y.,University of Chinese Academy of Sciences | Luo C.,CAS Guangzhou Institute of Geochemistry | Li J.,CAS Guangzhou Institute of Geochemistry | And 3 more authors.
Environmental Pollution | Year: 2011

The concentration and composition of PBDEs in the soils and plants near a typical e-waste recycling site in South China were investigated. The total concentration of PBDEs (ΣPBDEs) in soil ranged from 4.8 to 533 ng/g dry wt. The ΣPBDEs in vegetation were from 2.1 to 217 ng/g dry wt. For the vegetable, the highest concentration of 19.9 ng/g dry wt. was observed in the shoot of Brassica alboglabra L. BDE 209 was the predominant congener in all samples. In comaprison with other e-waste contaminated sites in China, lower concentrations of PBDEs and higher concentrations of PCBs were observed in both soils and plants suggesting different e-waste types involved in the present study. The PBDEs contaminated vegetables around the e-waste dismantling site may pose a potential health risk to the local inhabitants. © 2010 Published by Elsevier Ltd.

Groenewold N.,University of Western Australia | Chen A.,Jinan University | Lee G.,Xian Jiaotong University
Regional Studies | Year: 2010

Groenewold N., Chen A. and Lee G. Inter-regional spillovers of policy shocks in China, Regional Studies. Inter-regional output spillovers from policy shocks are analysed in a three-region vector-autoregressive (VAR)-based model of China using investment as the policy variable. The contemporaneous effect of policy is greater in the coastal region than in the other two regions, and the effect in the central region is larger than in the western region, suggesting that at least part of the expenditure boosts in the poorer inland regions find their way to the coastal provinces. These results are confirmed when the effects of the policy shocks are simulated over time and are found to be generally robust to alternative model specifications. © 2010 Regional Studies Association.

Wang Y.,CAS Guangzhou Institute of Geochemistry | Wang Y.,University of Chinese Academy of Sciences | Luo C.-L.,CAS Guangzhou Institute of Geochemistry | Li J.,CAS Guangzhou Institute of Geochemistry | And 3 more authors.
Chemosphere | Year: 2011

This study aimed at identifying the levels of PCBs generated from e-waste recycling, and their potential impacts on the soils and vegetations as well. The ΣPCBs concentrations in soil and plant samples ranged from 7.4 to 4000ngg -1 and from 6.7 to 1500ngg -1, respectively. For the plant samples, Chrysanthemum coronarium L. from vegetable field and the wild plant Bidens pilosa L. from the burning site showed relatively higher PCB concentrations than other species. For the soil samples, the e-waste burning site had relatively higher PCB concentrations than the adjacent areas, and vegetable soils had higher PCB concentrations than paddy soils. The PCB concentrations showed a clear decreasing trend with the increasing distance from the e-waste recycling site. PCB 28, 99, 101, 138, 153, and 180 were the predominant congeners. Principal component analysis results showed a potential fractionation of PCB compositions from the burning site to the surroundings. The PCB congener pattern at the burning site was similar to Arochlor 1260, pointing to an input of non-domestic e-waste. Similar PCB congeners were found in soils and related vegetables, indicating they derived from the same source. The consumption of vegetables grown in soils near e-waste recycling sites should be strictly avoided due to the high PCBs in the plant tissues. © 2011 Elsevier Ltd.

Xu Y.,Tongji University | He J.,Tongji University | Wang Y.,Tongji University | Zhu X.,Tongji University | And 3 more authors.
FEBS Letters | Year: 2015

MicroRNAs have been reported to play critical roles in various cancers, but there has been no study on the role of miR-889 in cancers. Here, we report that over-expression of miR-889 leads to rapid proliferation of EC109 and EC9706 cells in vitro and in vivo by inducing cells into S-phase. Using bioinformatics methods, DAB2IP was further confirmed to be a direct target of miR-889. In addition, the expression of DAB2IP, which was negatively correlated with that of miR-889, was significantly associated with clinicopathological features of ESCC patients. In conclusion, miR-889 is an important regulator in ESCC and both miR-889 and DAB2IP may serve as promising biomarkers and therapeutic targets in patients with ESCC. © 2014 Federation of European Biochemical Societies.

Liang H.,Guangdong Polytechnic Normal University | Huang J.,Jinan University | Zhao Y.,Sun Yat Sen University
Nonlinear Dynamics | Year: 2014

This paper is devoted to the complete classification of global phase portraits of quasi-homogeneous but non-homogeneous coprime planar polynomial differential systems of degree (Formula present). To prove our result, we firstly study the canonical forms for these systems. Then, the global topological structures of the systems having canonical forms are studied by using the quasi-homogeneous blow-up technique for the finite singularities and the Poincaré-Lyapunov compactification for the infinite singularities. Finally we perform a topological classification for the set of global phase portraits. © 2014, Springer Science+Business Media Dordrecht.

Ye L.,Wenzhou Medical College | Su Z.-J.,Jinan University | Ge R.-S.,Wenzhou Medical College | Ge R.-S.,Rockefeller University
Molecules | Year: 2011

The Leydig cells of the testis have the capacity to biosynthesize testosterone from cholesterol. Testosterone and its metabolically activated product dihydrotestosterone are critical for the development of male reproductive system and spermatogenesis. At least four steroidogenic enzymes are involved in testosterone biosynthesis: Cholesterol side chain cleavage enzyme (CYP11A1) for the conversion of cholesterol into pregnenolone within the mitochondria, 3β-hydroxysteroid dehydrogenase (HSD3B), for the conversion of pregnenolone into progesterone, 17a-hydroxylase/17,20-lyase (CYP17A1) for the conversion of progesterone into androstenedione and 17β-hydroxysteroid dehydrogenase (HSD17B3) for the formation of testosterone from androstenedione. Testosterone is also metabolically activated into more potent androgen dihydrotestosterone by two isoforms 5a-reductase 1 (SRD5A1) and 2 (SRD5A2) in Leydig cells and peripheral tissues. Many endocrine disruptors act as antiandrogens via directly inhibiting one or more enzymes for testosterone biosynthesis and metabolic activation. These chemicals include industrial materials (perfluoroalkyl compounds, phthalates, bisphenol A and benzophenone) and pesticides/biocides (methoxychlor, organotins, 1,2-dibromo-3-chloropropane and prochloraz) and plant constituents (genistein and gossypol). This paper reviews these endocrine disruptors targeting steroidogenic enzymes. © 2011 by the authors; licensee MDPI, Basel, Switzerland.

Liu G.-Y.,Guangdong University of Technology | Hu J.-M.,Wuhan University | Wang H.-L.,Jinan University
Scientometrics | Year: 2012

The aim of this study is to map the intellectual structure of digital library (DL) field in China during the period of 2002-2011. Co-word analysis was employed to reveal the patterns of DL field in China through measuring the association strength of keywords in relevant journals. Data was collected from Chinese Journal Full-Text Database during the period of 2002-2011. And then, the co-occurrence matrix of keywords was analyzed by the methods of multivariate statistical analysis and social network analysis. The results mainly include five parts: seven clusters of keywords, a two-dimensional map, the density and centrality of clusters, a strategic diagram, and a relation network. The results show that there are some hot research topics and marginal topics in DL field in China, but the research topics are relatively decentralized compared with the international studies. © 2011 Akadémiai Kiadó, Budapest, Hungary.

Tang S.,Jinan University
Pakistan Journal of Medical Sciences | Year: 2013

Traumatic lumbar spondylolisthesis is a rare lesion and frequently noted in patients with multiple traumatic injuries. We report one case of L5 traumatic spondylolisthesis, which obtained successful decompression, reduction, interbody fusion and fxation by posterior lumbar interbody fusion, and got satisfactory outcome. We recommend early decompression, reduction, interbody fusion and fxation with posterior instrumentation to obtain the recovery of neurological function and stability of the spine.

Hu Y.,Chinese Academy of Sciences | Cheng W.,Chinese Academy of Sciences | Cai W.,Jinan University | Yue Y.,Chinese Academy of Sciences | And 3 more authors.
Clinical Rheumatology | Year: 2013

At present, rheumatoid arthritis (RA) is considered a type of autoimmune disease. Its pathology is not certain, and effective drugs with less toxicity have not been established. The establishment and application of animal models are effective methods for RA research, especially using animal models similar to humans. Arthritis is more heterogeneous, and this is an important starting point when discussing animal models for arthritis. Animal models are instrumental in understanding the etiology and pathogenetic mechanisms of RA. Appropriate animal models should be selected according to experiments because they have different traits. Various methods have been applied to induce arthritis in animal experimental models, which have provided important insights into the etiopathogenetic mechanisms of human RA. This review was written to give a broad introduction of the current stage of RA model and hope to offer beneficial help for RA-related research. © 2012 Clinical Rheumatology.

Yin J.,Jinan University
International Journal of Robust and Nonlinear Control | Year: 2015

This paper investigates asymptotic stability in probability and stabilization designs of discrete-time stochastic systems with state-dependent noise perturbations. Our work begins with a lemma on a special discrete-time stochastic system for which almost all of its sample paths starting from a nonzero initial value will never reach the origin subsequently. This motivates us to deal with the asymptotic stability in probability of discrete-time stochastic systems. A stochastic Lyapunov theorem on asymptotic stability in probability is proved by means of the convergence theorem of supermartingale. An example is given to show the difference between asymptotic stability in probability and almost surely asymptotic stability. Based on the stochastic Lyapunov theorem, the problem of asymptotic stabilization for discrete-time stochastic control systems is considered. Some sufficient conditions are proposed and applied for constructing asymptotically stable feedback controllers. Copyright © 2014 John Wiley & Sons, Ltd.

Cai Y.,Jinan University | Cai T.-G.,Liaoning University
Chemical and Pharmaceutical Bulletin | Year: 2010

Phytochemical investigation of 90% ethanol extracts of the roots of Aristolochia fangchi yielded two new aristolochic acid derivatives named Aristolochic Acid F and Aristolochic Acid G, together with three known compounds. Their structures were elucidated by spectral analysis. The cytotoxicity of the isolated compounds was also determined. © 2010 Pharmaceutical Society of Japan.

Liang N.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To investigate the chemical constituents of Elephantopus mollis. Compounds were separated and purified by various column chromatographies including macroporous resin, ODS, Sephadex LH-20 and silica gel columns. The structures were identified by their physicochemical properties and spectroscopic data. Nine compounds were identified as 2beta-deethoxy-2-hydroxyphantomolin (1), 2beta-methoxy-2-deethoxyphantomolin (2), 2beta-methoxy-2-deethoxy-8-O-deacylphantomolin-8-O-tigli-nate (3), molephantinin (4), betulinic acid (5), magnolol (6), honokiol (7), dibutly phthalate (8) and tricin (9). Compounds 5-9 are isolated from this plant for the first time.

He Z.,Jinan University
Journal of Software | Year: 2011

In this paper, a gesture recognition system based on single tri-axis accelerometer mounted on a cell phone is proposed. We present a novel human computer interaction for cell phone through recognizing seventeen complex gestures. A new feature fusion method for gesture recognition based on time-domain and frequency-domain is proposed. First of all, we extract the time-domain features from acceleration data, that is short-time energy. Secondly, we extract the hybrid features which combine Wavelet Packet Decomposition with Fast Fourier Transform. Finally, we fuse these two categories features together and employ the principal component analysis to reduce dimension of fusion features. The Classifier we used is Multi-class Support Vector Machine. The average recognition results of seventeen complex gestures using the proposed fusion feature are 89.89%, which better than previous works. The performance of experimental results show that gesturebased interaction can be used as a novel human computer interaction for mobile device and consumer electronics. ©2011 ACADEMY PUBLISHER.

Tang S.,Jinan University
Pakistan Journal of Medical Sciences | Year: 2013

Pulmonary embolism is a fatal complication in orthopaedics surgery. While, the incidence of this lifethreatening condition is low in spine surgery and few detailed reports have been published in English literatures. We present a case of pulmonary embolism which occurred after a single-stage, combined anterior and posterior approach surgery for L2 burst fracture. Although positive and timely rescue measures were performed, the patient died finally. We report the case to help spine surgeons to be aware of and take precautions against the fatal condition in spine surgery.

Yin J.,Jinan University | Khoo S.,Deakin University
International Journal of Robust and Nonlinear Control | Year: 2015

This paper is concerned with the problem of finite-time stabilization for some nonlinear stochastic systems. Based on the stochastic Lyapunov theorem on finite-time stability that has been established by the authors in the paper, it is proven that Euler-type stochastic nonlinear systems can be finite-time stabilized via a family of continuous feedback controllers. Using the technique of adding a power integrator, a continuous, global state feedback controller is constructed to stabilize in finite time a large class of two-dimensional lower-triangular stochastic nonlinear systems. Also, for a class of three-dimensional lower-triangular stochastic nonlinear systems, a recursive design scheme of finite-time stabilization is given by developing the technique of adding a power integrator and constructing a continuous feedback controller. Finally, a simulation example is given to illustrate the theoretical results. Copyright © 2014 John Wiley & Sons, Ltd.

Xiang S.,Jinan University
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2013

This paper aims at reducing the embedding distortion by improving predictor's performance for prediction-error expansion (PE) based reversible watermarking. In the existing PE embedding methods, the predicted values or their variety should be rounded to integer values. This will restrict predictor's performance since the prediction context is only with past pixels (image) or samples (audio). In this paper, we propose a non-integer PE (NIPE) embedding approach, which can proceed non-integer prediction errors for data embedding by only expanding integer element of a prediction error while keeping its fractional element unchanged. More importantly, the NIPE scheme allows the predictor to estimate the current pixel/sample not restricted only past pixels/samples. We also propose a novel noncausal prediction strategy by combining past and future pixels/samples as the context. Experimental results for some standard test clips show that the non-integer output of predictor provides higher prediction performance, and the proposed NIPE scheme with the new predicting strategy can reduce the embedding distortion for the same payload. © 2013 Springer-Verlag.

Jia Y.B.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2012

To study the changes in the frontal white matter, the head of the hippocampus, and the anterior cingulate fasciculus metabolites in first-episode patients with positive symptoms of schizophrenia. Twenty first-episode patients with positive symptoms of schizophrenia underwent diffusion tensor imaging (DTI) and proton multi-voxel spectroscopy (1H-MRS) examination. 1H-MRS images were obtained from two sides of the frontal white matter, the head of the hippocampus and the anterior cingulate fasciculus regions. The metabolites detected included N-acetyl aspartate (NAA), choline-containing compounds (Cho), creatine and phosphocreatine (Cr), and the ratios of NAA/Cr, Cho/Cr and NAA/Cho were determined. The fractional anisotropy (FA) values were measured in the frontal white matter, the head of the hippocampus, and the anterior cingulate fasciculus. The Cho/Cr ratio was significantly reduced in the left frontal white matter (1.18 ± 0.21) of the patients as compared to the right side (1.44 ± 0.34, P<0.05). In the left head of the hippocampus, NAA/Cr (1.34 ± 0.45) and Cho/Cr (1.41 ± 0.39) were significantly reduced compared to those of the right side (1.75 ± 0.15 and 1.76 ± 0.36, respectively, P<0.05). No significant differences in the metabolite levels were found between the left and right anterior cingulate fasciculus (P>0.05). DTI showed similar FA values between the left and right sides of the frontal white matter, the head of the hippocampus, and the anterior cingulate fasciculus (P>0.05). Patients with positive symptoms of schizophrenia have diminished neuronal integrity and/or function in the left frontal white matter and head of the hippocampus, but not in the anterior cingulate fasciculus.

Jin W.,Hong Kong Polytechnic University | Xuan H.,Hong Kong Polytechnic University | Jin L.,Jinan University
Journal of Lightwave Technology | Year: 2013

We report novel rocking long period gratings (R-LPGs) made by introducing permanent periodic twist along a standard single mode fiber. Very high coupling efficiency of 32.5 dB was achieved with 23 periods and a 2° rocking angle. The responses of R-LPGs to temperature, strain, and torsion were tested. Compared with a normal LPG written under the same conditions, the R-LPGs were found to have a similar sensitivity to temperature, five times smaller sensitivity to strain, and insensitive to twist. © 1983-2012 IEEE.

Wang Z.X.,Jinan University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011

To assess the DNA damage in mouse sperms induced by exogenous BDE-209 and explore the possible mechanism of BDE-209 in affecting normal zygote development. Mouse sperms were harvested from the epididymal tail and suspended in HTF medium for a 90-min exposure to BDE-209 at varied concentrations of 0, 2.5, 5.0, 10, and 20 μg/ml (groups A-E, respectively). After the exposure, the sperms were subjected to single-cell gel electrophoresis (SCGE) to assess the DNA damage. The tail length of the sperms averaged 1.15 ∓ 1.27 μm in group A. Exposure to 10 and 20 μg/ml BDE-209 resulted in a significant lengthening of the sperm tails (2.13 ∓ 1.29 μm and 2.83 ∓ 2.46 μm, respectively, P<0.01) as well as increased DNA content in the tail of the cells (P<0.01). The Olive tail moment in group A was 0.270 ∓ 0.322, and increased after BDE-209 exposure to 0.453 ∓ 0.375 and 808 ∓ 0.822 in groups D and E, respectively. The tail/head length ratio in groups C, D, and E (0.077 ∓ 0.093, 0.112 ∓ 0.068, and 0.191 ∓ 0.207) were significantly greater than that in group A (0.045 ∓ 0.049). The DNA damage of the mouse sperms was directly correlated to the concentrations of BDE-209, with correlation coefficients all above 0.9. Exogenous BDE-209 can cause mouse sperm DNA damage and lead to sperm DNA chain breakage, and this effect shows an obvious dose dependence.

Cheng K.-W.,University of Hong Kong | Wong C.-C.,University of Hong Kong | Wang M.,University of Hong Kong | He Q.-Y.,Jinan University | Chen F.,University of Hong Kong
Mass Spectrometry Reviews | Year: 2010

Natural products, and their derivatives and mimics, have contributed to the development of important therapeutics to combat diseases such as infections and cancers over the past decades. The value of natural products to modern drug discovery is still considerable. However, its development is hampered by a lack of a mechanistic understanding of their molecular action, as opposed to the emerging molecule-targeted therapeutics that are tailored to a specific protein target(s). Recent advances in the mass spectrometry-based proteomic approaches have the potential to offer unprecedented insights into the molecular action of natural products. Chemical proteomics is established as an invaluable tool for the identification of protein targets of natural products. Small-molecule affinity selection combined with mass spectrometry is a successful strategy to "fish"cellular targets from the entire proteome. Mass spectrometry-based profiling of protein expression is also routinely employed to elucidate molecular pathways involved in the therapeutic and possible toxicological responses upon treatment with natural products. In addition, mass spectrometry is increasingly utilized to probe structural aspects of natural products-protein interactions. Limited proteolysis, photoaffinity labeling, and hydrogen/deuterium exchange in conjunction with mass spectrometry are sensitive and high-throughput strategies that provide low-resolution structural information of non-covalent natural product-protein complexes. In this review, we provide an overview on the applications of mass spectrometry-based techniques in the identification and characterization of natural product-protein interactions, and we describe how these applications might revolutionize natural product-based drug discovery. © 2009 Wiley Periodicals, Inc.

Tan Y.-N.,Dalian University of Technology | Zhang Y.,Dalian University of Technology | Guan B.-O.,Jinan University
IEEE Sensors Journal | Year: 2011

A hydrostatic pressure insensitive fiber-optic hydrophone based on the integration of a dual polarization fiber grating laser and an elastic diaphragm is proposed and experimentally demonstrated. The diaphragm converts acoustic pressure into transversal force onto the fiber laser but shows no response to the hydrostatic pressure. Therefore, the beat frequency is sensitive to acoustic signal but insensitive to the hydrostatic pressure. © 2011 IEEE.

Lin Z.,Jinan University | Zhang Z.,Sun Yat Sen University | Mai K.,Sun Yat Sen University
Journal of Applied Polymer Science | Year: 2012

In this article, a novel eggshell/β-PP bio-composite was prepared and evaluated. First the waste chicken eggshell (ES) was modified by a stingy amount of pimelic acid (PA) through solution method, and then compounded with PP through melt blending method. The crystalline form, mechanical properties, and morphology of the ES/β-PP composites were studied. The results showed that ES modified by PA was a β nucleating agent with high efficiency and selectivity, resulting in the maximum K β value of 0.99 in PP composites. PA modification promoted the dispersion and interfacial bonding of ES in PP. Although the addition of PA-modified ES slightly decreased tensile properties and flexural properties of PP, it increased the impact strength of PP by 228% than that of pure PP. Taking all factors into consideration, the optimal mass ratio of PP/ES/PA composites for reaching optimum mechanical properties should be 100/5/0.000471. Thus, the application of modified ES to prepare ES/β-PP bio-composites represents a promising way to mitigate environmental pollution and to reduce the cost of polyolefin products. Copyright © 2011 Wiley Periodicals, Inc.

Pan D.,Jinan University
2010 International Conference on Machine Learning and Cybernetics, ICMLC 2010 | Year: 2010

The role of data mining has become increasingly important for organizational marketing that has large databases of information on customers. The data mining researchers have presented various mining algorithms to extract patterns in data for successful CRM approach. The traditional dominant KDD process model offers valuable guidance on the projected-management oriented stages, with no relevant mechanism to provide guidance in the crucial issue of business understanding, model selection and known knowledge integration. The requirements of continuous data mining process for marketing and CRM environment are described, and then a novel integrative framework for knowledge discovery process is proposed, and a knowledge conceptual hierarchy to characterize the process operation features and interrelations is discussed. © 2010 IEEE.

Objective: To develop and verify the procedure for virus inactivation/removal in recombinant human interleukin-12 (rhIL-12). Methods: A procedure for virus removal in rhIL-12 was developed by using pre-filter (Viresolve Shield, nano film 3.1 cm2) combined with the filter for parvovirus (Viresolve Pro, nano film 3.1 cm2). The volume of sample was 90 ml, while the time for filtration was 2 h, the safety coefficient was 1.88 and the pressure was 30 psi. The procedure was analyzed for effects on specific activity and recovery of product, and verified for efficacy of virus removal using porcine parvovirus (PPV) as an indicator. A procedure for virus inactivation in rhIL-12 was developed by low pH incubation, in which the pH value was adjusted to 3.8 ± 0.1 with 1 mol/L hydrochloric acid and, after incubation at room temperature for 180 min, to 7.4 with 1 mol/L sodium hydroxide. The procedure was analyzed for effect on content and specific activity of product, and verified for the efficacy of virus inactivation using vesicular stomatitis virus (VSV) as an indicator. Results: The recovery rate of protein in the developed virus removal procedure was 95.14%, while the specific activity was 95.12% of that before filtration. The procedure showed little influence on rhIL-12 activity. When the volume of filtrate reached 90 ml, the titer of PPV decreased by more than 4 log10, which met the General Requirements for Technology of Evaluation on Safety of Virus in Tissue Extracts and Expressed Products in Eukaryotic Cells. However, in the virus inactivation procedure, the protein contents before and after virus inactivation were 359 and 338 μg/ml, while the biological activities were 3.392 × 106 and 3.356 × 106 IU, respectively, which showed no significant change. After treatment at room temperature for 180 min, both the titers of PRV and VSV decreased by 4 log10, which met the Requirements for Technology of Evaluation on Safety of Virus in Tissue Extracts and Expressed Products in Eukaryotic Cells. Conclusion: The developed procedures were effective in inactivation/removal of viruses in rhIL-12, which ensured the quality and safety of products.

Cheng B.,General Hospital of Guangzhou Military Command | Liu H.-W.,Jinan University | Fu X.-B.,Institute for Basic Research
Journal of Burn Care and Research | Year: 2011

Although itching (or pruritus) in a scar is a very common and distressing symptom and is increasingly being recognized as a significant obstacle in burn rehabilitation, the exact mechanisms underlying this symptom have not been elucidated; hence, a reliable therapy has not been established. Recent findings have suggested that itching caused by inflammatory dermatosis can be reduced by antihistamines, but histamine antagonists cannot block all types of pruritus (eg, neuropathic itch). This indicates the presence of a histamine-independent pathway for itch. Itch or pruritus may also be evoked by direct activation of opioid receptors, which have recently been identified in the skin. This article aims to assess the current state of knowledge regarding the role of opioids in the generation of itch in hypertrophic scars in postburn patients. To this end, the authors have reviewed the relevant literature and present some clinical data. The authors hope that this review will form the basis for future research to elucidate the mechanism and treatment of itch. Copyright © 2011 by the American Burn Association.

Liu D.,Jinan University
Proceedings - 2013 8th International Conference on P2P, Parallel, Grid, Cloud and Internet Computing, 3PGCIC 2013 | Year: 2013

Cloud computing has attracted widespread attentions in recent years. Real-time Online Interactive Application (ROIA) is also an emerging distributed application. And the research on cloud ROIA is just beginning. Traditionally, Dead Reckoning (DR) algorithm will be used to reduce network traffic and mitigate consistency problem caused by network delay in ROIA. But traditional DR algorithm is mostly based on the classic formula of physics to predict, without taking the influence of the user's goal under the different situations into account, so there are some limitations. This paper proposes an improved DR algorithm based on target-predicting in a cloud platform for ROIA, and elaborates the basic idea of the improved algorithm and the computational model formula, and then carries out a simulation experiment. The analyses of the simulation results show that the improved algorithm is superior to traditional one. And the result of this study sheds a little light to the related further research on cloud ROIA. © 2013 IEEE.

Zhang W.,South China Normal University | Wang X.,Jinan University | Chen T.,South China Normal University
Molecular and Cellular Biochemistry | Year: 2011

Resveratrol (RV), a natural plant polyphenol widely present in foods such as grapes, wine, and peanuts, has an ability to inhibit various stages of carcinogenesis in vitro and in vivo. In this report, we explored the roles of intrinsic and extrinsic apoptotic pathways during RV-induced apoptosis in human lung adenocarcinoma (ASTC-a-1) cells. After exposure of cells to different concentrations of RV, we found that RV induced concentration-dependent apoptosis. Fluorometric substrates assay and western blotting (WB) analysis showed that caspase-8 was not activated, which was further verified by monitoring the cleavage of Bid to tBid using fluorescence resonance energy transfer (FRET) microscopy imaging inside single living cells, indicating that extrinsic apoptotic pathway was not involved in RV-induced apoptosis. In addition, inhibition of caspases-3 or -9 but not caspase-8 using the specific inhibitors of caspases modestly but significantly attenuated RV-induced apoptosis. Moreover, flow cytometry (FCM) analysis showed that RV treatment induced time-dependent loss of mitochondrial membrane potential (Δψm), in combination with the activation of caspases-3 and -9; we therefore concluded that RV-induced apoptosis involved the intrinsic apoptotic pathway. It is noteworthy that RV treatment induced translocation of AIF from mitochondria to nucleus in a time dependent manner, and that knockdown of AIF remarkably attenuated RV-induced apoptosis. Collectively, our findings demonstrate that RV induces caspase-8-independent apoptosis via AIF and to a lesser extent caspase-9-dependent mitochondrial pathway in ASTC-a-1 cells. © 2011 Springer Science+Business Media, LLC.

Huang F.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To study the inhibitory effect of Luteolin on LPS-induced BV-2 cell. BV-2 cells were treated with LPS (0.1 microg/mL) for inflammation model; MTT assay was used to detect the viability of BV-2 cells; Nitric oxide (NO) was detected by the method of nitric acid reductase assay; Induce type II nitric oxide synthase (iNOS) enzyme activity was determined by type of nitric oxide synthase assay;TLR4 protein expression was examined by the Western Blot analysis. Luteolin significantly decreased the NO production and TLR4 protein expression as well as iNOS activity in LPS-activated microglial cell. LPS induced activation of microglia lead to inflammatory response and its mechanism may be related to inhibiting TLR4 signaling pathway.

Hu D.,Jinan University | Tateno H.,Japan National Institute of Advanced Industrial Science and Technology | Hirabayashi J.,Japan National Institute of Advanced Industrial Science and Technology
Molecules | Year: 2015

In the post genomic era, glycomics - the systematic study of all glycan structures of a given cell or organism - has emerged as an indispensable technology in various fields of biology and medicine. Lectins are regarded as "decipherers of glycans", being useful reagents for their structural analysis, and have been widely used in glycomic studies. However, the inconsistent activity and availability associated with the plant-derived lectins that comprise most of the commercially available lectins, and the limit in the range of glycan structures covered, have necessitated the development of innovative tools via engineering of lectins on existing scaffolds. This review will summarize the current state of the art of lectin engineering and highlight recent technological advances in this field. The key issues associated with the strategy of lectin engineering including selection of template lectin, construction of a mutagenesis library, and high-throughput screening methods are discussed. © 2015 by the authors; licensee MDPI.

Jiang G.-M.,University of South China | Jiang G.-M.,Sun Yat Sen University | Wang H.-S.,Sun Yat Sen University | Zhang F.,Sun Yat Sen University | And 6 more authors.
Biochimica et Biophysica Acta - Molecular Cell Research | Year: 2013

Histone deacetylase inhibitors (HDACIs) are now emerging as a new class of anticancer drugs. Some of them have been used in clinical treatment for tumors, most impressively in the hematological tumors. But their single-agent activities in epithelial-derived tumors are limited. The mechanisms of these actions of HDACIs are not yet well understood. In this study, it was found for the first time that HDACIs were able to induce epithelial-mesenchymal transitions (EMT) which is believed to trigger tumor cell invasion and metastasis. We show that HDACIs induce fibroblast-like morphology, up-regulate Snail and Vimentin and down-regulate E-cadherin in epithelial cell-derived tumor cell lines. It demonstrates that HDACI treatment enhances further Snail acetylation and reduces its ubiquitylation, and induces Snail transcription as well as Snail nuclear translocation in CNE2 cells. Snail knockdown by siRNAs prevents the change in cell morphology and Vimentin up-regulation in response to HDACIs. The results suggested that Snail plays an important role in the HDACI-induced EMT. It is very crucial for a better understanding of clinical therapeutical failure of HDACIs in the patients with epithelial cell-derived cancers. Therefore, our results indicate that more attention should be paid to the cancer treatment using HDACIs due to the fact that it will enhance the spread risks of cancer cells to facilitate cancer progression and it is very important to select appropriate drugs for different tumors. © 2012 Elsevier B.V.

Sun L.,University of Jinan | Wang S.,Harvard University | Hu C.,Jinan University | Zhang X.,University of Jinan
Experimental Cell Research | Year: 2011

Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell proliferation and apoptosis, it is still unclear how apoptosis is regulated. The aim of this study is to analyze the relationship among apoptosis, phosphatidylinositol 3-kinase (PI3K)/Akt and nuclear factor κB (NF-κB) in FPC knockdown kidney cells. We show that PKHD1-silenced HEK293 cells demonstrate a higher PI3K/Akt activity. Selective inhibition of PI3K/Akt using LY294002 or wortmannin in these cells increases serum starvation-induced HEK293 cell apoptosis with a concomitant decrease in cell proliferation and higher caspase-3 activity. PI3K/Akt inhibition also leads to increased NF-κB activity in these cells. We conclude that the PI3K/Akt pathway is involved in apoptotic function in PKHD1-silenced cells, and PI3K/Akt inhibition correlates with upregulation of NF-κB activity. These observations provide a potential platform for determining FPC function and therapeutic investigation of ARPKD. © 2011 Elsevier Inc.

Liu P.,University of Turku | Kallio H.,University of Turku | Kallio H.,Jinan University | Lu D.,ShenYang Agricultural University | And 2 more authors.
Food Chemistry | Year: 2011

Eleven major phenolic compounds (hyperoside, isoquercitrin, chlorogenic acid, ideain, epicatechin, two procyanidin (PA) dimers, three PA trimers and a PA dimer-hexoside) were quantified in the fruits of 22 cultivars/origins of three species of the Chinese hawthorn (Crataegus spp.) by HPLC-ESI-MS-SIR. Hyperoside (0.1-0.8 mg/g dry mass [DM]), isoquercitrin (0.1-0.3 mg/g DM), chlorogenic acid (0.2-1.6 mg/g DM), epicatechin (0.9-11.7 mg/g DM), PA B2 (0.7-12.4 mg/g DM), PA dimer II (0.1-1.5 mg/g DM), PA trimer I (0.1-2.7 mg/g DM), PA trimer II (0.7-6.9 mg/g DM), PA trimer III (0.01-1.2 mg/g DM) and a PA dimer-hexoside (trace-1.1 mg/g DM) were detected in all the samples. Ideain (0.0-0.7 mg/g DM) was found in all the samples except Crataegus scabrifolia. Significant correlations between the contents of individual PA aglycons were observed (r > 0.9, P < 0.01). A strong correlation between flavonols was also shown (r = 0.71, P < 0.01). Fruits of Crataegus pinnatifida var. major had higher contents of PAs but lower contents of flavonols compared with Crataegus brettschneideri. The fruits of C. scabrifolia contained the highest level of PA dimer-hexoside, which was present in trace amounts in the fruits of C. pinnatifida. © 2011 Elsevier Ltd. All rights reserved.

Xiao F.,South China Normal University | Gao W.,South China Normal University | Wang X.,Jinan University | Chen T.,South China Normal University
Apoptosis | Year: 2012

Although caspases have been demonstrated to be involved in artemisinin (ARTE)-induced apoptosis, their exact functions are not well understood. The aim of this report is to explore the roles of caspase-8, -9 and -3 during ARTE-induced apoptosis in human lung adenocarcinoma (ASTC-a-1) cells. ARTE treatment induces a rapid generation of reactive oxygen species (ROS), and ROS-dependent apoptosis as well as the activation of caspase-8, -9 and -3 via time- and dose-dependent fashion. Of upmost importance, inhibition of caspase-8 or -9, but not caspase-3, almost completely blocks the ARTE-induced not only activation of the caspase-8, -9 and -3 but also apoptosis. In addition, the apoptotic process triggered by ARTE does not involve the Bid cleavage, tBid translocation, significant loss of mitochondrial membrane potential and cytochrome c release from mitochondria. Moreover, silencing Bax/Bak does not prevent the ATRE-induced cell death as well as the activation of caspase-8, -9 and -3. Collectively, our data firstly demonstrate that ARTE triggers a ROS-mediated positive feedback amplification activation loop between caspase-8 and -9 independent of mitochondria, which dominantly mediated the ARTE-induced apoptosis via a caspase-3-independent apoptotic pathway in ASTC-a-1 cells. Our findings imply a potential to develop new derivatives from artemisinin to effectively initiate the amplification activation loop of caspases. © 2012 Springer Science+Business Media, LLC.

Yang Y.H.,Jinan University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Sinperca chuatsi usually refuses eating dead bait or man-made feed due to its specific feeding habit. It was showed that some S. chuatsi could be induced to take lifeless bait with domestication gradually after long-term of cultivation. High cost, serious pollution, severe diseases, and other problems of S chuatsi cultivation can be solved effectively by selecting those liable to domestication via molecular marker and treating them with artificial feed on a large scale. Lipoprotein lipase, one of the key enzymes in lipoprotein metabolic processes, provides energy by catalyzing the oxidation of triglycerides lie in the core of chylomicron and very low density lipoprotein (VLDL) into glycerin and fatty acid, and saves energy. In order to search the distribution of the alleles and genotypes of lipoprotein lipase gene (lipoprotein LPL) gene in two feed habit domestication phenotype populations, SNP genetic polymorphisms in 6 and 7 introns and 6, 7, and 8 exons of LPL in S. chuatsi were analyzed by PCR product sequencing method. Three SNPs A25T, G26T, and C29G were detected, two of which were non-synonymous mutations. It was indicated by Chi-square test that the analysis between domesticated and undomesticated populations show no significant difference (P>0.05) between the three SNPs and feed habit. However, diplotype 2 in the two population showed significant difference (P<0.05) by assembling different genotypes of the three SNPs into five diplotypes. Polymorphic analysis of partial fragment of LPL genome in S. chuatsi was accomplished successfully. Therefore, LPL gene can be considered as a candidate gene and genetic marker for feed habit domestication in S. chuatsi, which lays the foundation for the work of molecular marker and selecting breed, which has extensive application prospect.

To observe the effect of inhalation of aerosolized perfluorocarbon combined with tetramethylpyrazine on the hemodynamics and histopathology in a porcine model of acute lung injury. Normal adult pigs were subjected to saline lavage of the bilateral lungs to induce acute lung injury and randomized subsequently into 3 groups for treatment with inhalation of perfluorocarbon, combined inhalation of perfluorocarbon and tetramethylpyrazine, or inhalation of tetramethylpyrazine. The changes of mean arterial pressure (MAP), PetCO(2), mPAP, CVP and PAWP were recorded at different time points following the lung injury, and the lung tissues were sampled for histological observations. The MAP, mPAP, CVP and PAWP all increased significantly in the 3 groups after acute lung injury. Interventions with combined tetramethylpyrazine and perfluorocarbon inhalation significantly improved these indices as compared with inhalation of tetramethylpyrazine or perfluorocarbon alone (P<0.05). The pulmonary pathology was the mildest in the combined inhalation group, and the most severe in tetramethylpyrazine group. Combined inhalation of perfluorocarbon and tetramethylpyrazine can effectively improve the oxygenation, reduce pulmonary arterial pressure?and ameliorate lung pathology in pigs with acute lung injury.

Li W.,Guangzhou University | Wang Q.,Jinan University | Su Q.,Guangzhou University of Chinese Medicine | Ma D.,Guangzhou University of Chinese Medicine | And 3 more authors.
Molecules and Cells | Year: 2014

Renal cell carcinoma (RCC) is associated with a high frequency of metastasis and only few therapies substantially prolong survival. Honokiol, isolated from Magnolia spp. bark, has been shown to exhibit pleiotropic anticancer effects in many cancer types. However, whether honokiol could suppress RCC metastasis has not been fully elucidated. In the present study, we found that honokiol suppressed renal cancer cells' metastasis via dual-blocking epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties. In addition, honokiol inhibited tumor growth in vivo. It was found that honokiol could upregulate miR-141, which targeted ZEB2 and modulated ZEB2 expression. Honokiol reversed EMT and suppressed CSC properties partly through the miR-141/ZEB2 axis. Our study suggested that honokiol may be a suitable therapeutic strategy for RCC treatment. © The Korean Society for Molecular and Cellular Biology. All rights reserved.

Li Z.,Baylor College of Medicine | Li Z.,Jinan University | Burns A.R.,Baylor College of Medicine | Burns A.R.,University of Houston | And 5 more authors.
American Journal of Pathology | Year: 2011

The contribution of acute inflammation to sensory nerve regeneration was investigated in the murine cornea using a model of corneal abrasion that removes the stratified epithelium and subbasal nerve plexus. Abrasion induced accumulation of IL-17+ CCR6+ γδ T cells, neutrophils, and platelets in the cornea followed by full restoration of the epithelium and ∼19% regeneration of sensory nerves within 96 hours. Mice deficient in γδ T cells (TCRδ-/-) or wildtype mice treated systemically with anti-IL-17 had >50% reduction in leukocyte and platelet infiltration and >50% reduction in nerve regeneration. Strategies used to prevent neutrophil and platelet accumulation (eg, wild-type mice treated with anti-Ly6G or anti-GP1bα antibody to deplete neutrophils or platelets) also resulted in >50% reductions in corneal nerve density. Infiltrating neutrophils and platelets stained positively for VEGF-A, tissue levels of VEGF-A peaked coincidentally with peak tissue levels of neutrophils and platelets, depletion of neutrophils before injury reduced tissue VEGF-A levels by >70%, and wild-type mice treated systemically with anti-VEGF-A antibody exhibited >80% reduction in corneal nerve regeneration. Given the known trophic effects of VEGF-A for neurite growth, the results in this report demonstrate a previously unrecognized beneficial role for the γδT cell-dependent inflammatory cascade involving IL-17, neutrophils, platelets, and VEGF-A in corneal nerve regeneration. Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

Sui W.,Jinan University
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia | Year: 2013

Transcription factors (TFs) play a central role in regulating gene expression and in providing an interconnecting regulatory between related pathway elements. Currently, the wide-spread use of kidney transplantation to treat end-stage renal disease has evolved rapidly since the initial successful transplantations from both cadaveric and living donors. However, acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate the possible mechanisms, we describe a comparison between TF' activity profile of acute rejection and controls. Through TF assay analysis and electrophoretic mobility shaft assay confirmation, we identified the activities of TFs in acute rejection after kidney transplantation. From a total of 345 screened TFs, 99 activity-differential TFs were found, of which 95 showed increased activity and four showed decreased activity. Our data indicate that TFs may be potentially involved in the pathogenesis of acute rejection, and can help to prevent, diagnose and treat acute rejection after kidney transplantation. The TF array methods could simplify the assay of multiple TFs and may facilitate high-throughout profiling of large numbers of TFs.

Luo M.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013

To prepare the monoclonal antibody against zearalenone (ZEN) toxin and preliminarily establish the colloidal gold immunochromatographic detection method for ZEN. The artificial antigen ZEN-BSA and ZEN-OVA were prepared by active ester method. Mice were immunized with ZEN-OVA and monoclonal antibodies against ZEN were prepared by regular cell fusion and subcloning approach. The titer, subtype and specificity of the antibodies were identified by ELISA. To establish a method of colloidal gold immunochromatographic assay (GICA) for the determination of ZEN, colloidal gold binding cushion was coated with anti-ZEN monoclonal antibody-colloidal gold complex, and the synthetic ZEN-BSA and goat anti-mouse immunoglobulins were sprayed on cellulose nitrate film to form the test (T) band and control (C) band. Identified by SDS-PAGE and UV spectroscopy, the artificial antigen ZEN-BSA and ZEN-OVA were successfully coupled respectively. One hybridoma line (1G4) which could secrete monoclonal antibody specifically against ZEN was obtained by three-time cloning. The ascites titer of this monoclonal antibody reached 1:1.6×10(5);. The antibody subtype was IgG2b. The IC50; to ZEN was 10.2 ng/mL, and the detction limit was 0.58 ng/mL. In addition, the mAb was proved highly specific for ZEN. ZEN metabolites α-zearalanol, β-zearalanol, zearalanone and other similar toxins deoxynivalenol, fumonisin B1, ochratoxin A showed very low or no cross-activity with the monoclonal antibody. The whole assay using the prepared colloidal gold immunochromatographic strip could be finished in 5 min, which could be read by naked eyes. The limit of detection was 100 ng/mL. The anti-ZEN mAb was successfully prepared. And the gold immunochromatographic assay(GICA) for ZEN was preliminarily established with the limit of detection being 100 ng/mL.

Lai X.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013

To investigate the effect of anhydroicaritin (AHI) against zymosan-induced peritonitis in mice. Peritonitis was induced in mice by intraperitoneal injection of zymosan. All mice were monitored for systemic toxicity and mortality for 13 d after zymosan or saline administration. In another set of experiments, the peritoneal exudates were collected. The leukocyte numbers and the production of inflammatory cytokines (IL-6, IL-10, TNF-α, MCP-1) were determined by flow cytometry. The release of nitric oxide (NO) was measured by a Griess reagent system. The Ca(2+); influx in bone marrow-derived macrophages was recorded by laser scanning confocal microscopy with Fluo4-AM loading. The expression of iNOS was determined by Western blotting. AHI (4 mg/kg) prolonged survival of peritonitis mice, inhibited massive leukocyte transmigration into the peritoneal cavity, and decreased the overproduction of NO, IL-10, TNF-α, MCP-1 and IL-6. In LPS-stimulated mouse macrophages, AHI (5 μmol/L) pretreatment significantly inhibited the elevation of intracellular Ca(2+);, and markedly decreased iNOS protein expression. AHI possesses significant protective effects on the zymosan-induced peritonitis mice, which might be associated with the regulation of Ca(2+); influx in macrophages and iNOS expression.

Ye S.S.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011

To investigate the effects of salidroside(Sal) on proliferation, apoptosis, phagocytosis, the production of ROS and NO of murine peritoneal macrophages in vitro as well as its immunoregulation. The single cell suspension of murine peritoneal macrophages was prepared under sterile condition, then co-cultured with different concentrations of Sal(80, 160 and 320 μmol/L)for 4 hours prior to stimulation with LPS and IFN-γ, the proliferation of macrophages was measured by MTT colorimetry. The effect of Sal on the apoptosis of Sytox® Green-labelled peritoneal macrophages induced by CHX was detected by Fluorescence enzyme-labelled meter. FCM was used to detect the effect of Sal on phagocytosis of peritoneal macrophages. Fluorescence enzyme-labelled meter was used to measure the effects of Sal on ROS of H(2);DCFDA-labelled macrophages induced by LPS and IFN-γ. Griess Gragent was used to detect the role of Sal in production of NO in peritoneal macrophages activated by LPS and IFN-γ. MTT result demonstrated that Sal could promote the proliferation of peritoneal macrophages activated by LPS and IFN-γ at the final concentrations of 80, 160, 320 μmol/L, respectively (P<0.05). The result of Fluorescence enzyme-labelled meter detected showed that Sal at the final concentration of 160 μmol/L could inhibit apoptosis of peritoneal macrophages induced by CHX(P<0.01). FCM analysis showed that different concentrations of Sal significantly promoted the phagocytosis of peritoneal macrophages which include un-activated and activated by LPS and IFN-γ(P<0.05). Fluorescence enzyme-labelled meter showed that Sal could reduce the production of ROS in activated peritoneal macrophages induced by LPS and IFN-γ(P<0.05). Sal also increased the production of NO in activated peritoneal macrophages induced by LPS and IFN-γ(P<0.05). Sal can promote proliferation of peritoneal macrophages stimulated by LPS and IFN-γ, and it can inhibit apoptosis of peritoneal macrophages induced by CHX, Sal also can promote the phagocytosis of peritoneal macrophages which include un-activated and activated by LPS and IFN-γ, Sal can reduce the production of ROS in activated peritoneal macrophages induced by LPS and IFN-γ, while Sal can promote the production of NO in activated peritoneal macrophages induced by LPS and IFN-γ.

Fang S.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013

To investigate c-Cbl and Cbl-b gene expressions in peripheral blood mononuclear cells (PBMCs) from multiple myeloma (MM) patients. SYBR(R); Green PCR technique was used to detect c-Cbl and Cbl-b gene expressions in PBMCs from 23 MM patients and 22 healthy individuals, and RT-PCR and DNA sequence analysis were performed to analyze the mutations of 7-10 exons of c-Cbl. The expression of c-Cbl gene in MM patients (median: 0.798%) significantly decreased as compared with that in healthy controls (median: 2.443%) (P<0.05). The expression of Cbl-b gene in MM patients (median: 0.714%) also dropped significantly as compared with that in healthy controls (median: 2.179%) (P<0.05). The 7-10 exons of c-Cbl gene had two different sizes of fragments in 2 MM patients: 483 bp and 148 bp which were wild-type and deletion mutants type of c-Cbl gene. c-Cbl gene mutations were not found in all MM patients. The expressions of c-Cbl and Cbl-b genes in PBMCs from MM patients are down-regulated.

Li Z.-J.,Jinan University | Ou-Yang P.-H.,South China Agricultural University | Han X.-P.,South China Agricultural University
Cellular Signalling | Year: 2014

MicroRNAs (miRNAs) attract more attention in the pathophysiology of liver fibrosis and miR-33a has been previously demonstrated as involved in the regulation of cholesterol and lipid metabolism. Transforming growth factor-beta1 (TGF-β1) is generally accepted to be the main stimulating factor in the hepatic stellate cells (HSCs) activation, which plays an important role in hepatic fibrosis. However, the involvement and underlying mechanism of miR-33a and its role in TGF-β1-induced hepatic fibrogenesis remains unknown. Here, we investigate the role of miR-33a in the activation of immortalized human HSCs, Lx-2 cells. Our findings have shown that the expression of miR-33a with its host gene sterol regulatory element-binding protein 2 (SREBP2) was more highly expressed in activation of Lx-2 cells than in quiescent cells. The expression of miR-33a on TGF-β1-induced HSCs activation may be modulated via the activation of PI3K/Akt pathway. In addition, miR-33a significantly correlated with TGF-β1-induced expression of α1 (I) collagen (Col1A1) and α-SMA in HSCs. Bioinformatics analyses predict that peroxisome proliferator activated receptor-alpha (PPAR-α) is the potential target of miR-33a. We further found that anti-miR-33a significantly increases target gene PPAR-α mRNA and protein level, suggesting that miR-33a involved in HSCs function might be modulated by targeting PPAR-α. Finally, our results indicate that the expression of miR-33a increased with the progression of liver fibrosis. These results suggested that anti-miR-33a inhibit activation and extracellular matrix production, at least in part, via the activation of PI3K/Akt pathway and PPAR-α and anti sense of miR-33a may be a novel potential therapeutic approach for treating hepatic fibrosis in the future. © 2013 Elsevier Inc.

Yan L.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011

To produce the monoclonal antibodies (mAb) against Heavy Metal Chromium, and develop a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) for the detection of Chromium. Chromium ions was chelated with bifunctional chelating agent Isothio-cyanobenzyl- EDTA firstly and then conjugated with bovin serum alburmin(BSA) and ovalbmin(OVA) respectively to complete antigens . Four female BALB/c mice were immunized with Cr-iEDTA-BSA, the Hybridoma lines secreting monoclonal antibodies against Chromium ions were established by the hybridoma technology. The titer and the specificity of the antibodies were characterized in the way of indirect enzym-elinked immunosorbent assay (iELISA) and ciELISA. After immunization and cell fusion, three hybridomaes which can stably secrete mAbs against chromium were obtained. The antibody titer was up to 1×10(5);, only had 9.62% cross-reactivity to Fe(3+); and little to other ions. Linear detection of ciELISA for Chromium covered a range from 0.783 μg/L-50 μg/L with the limit at the level of 1 μg/L. An excellent correlation of results obtained by ciELISA and ICP-AES when the concentration more than 1 μg/L. The monoclonal antibody against Chromium with high sensitivity and specificity has been generated and a competitive inhibition enzyme-linked immunosorbent assay for the detection of Chromium ions has been established successfully, which was met the the national standard of water quality nonitoring.

Chen Z.-D.,Jinan University
Physical Review A - Atomic, Molecular, and Optical Physics | Year: 2015

Because of the inherent coupling, the influence from the phonon bath on the coherent energy exchange between a quantum-dot (QD) two-state system (TSS) and a resonant optical mode is a twofold action. One action is the influence on the construction of the coherence, and the other is on the constructed coherence. The latter has been extensively studied by the master-equation approach, whereas the former has been ignored so far. In the present paper, the influence from the phonon bath on the construction of the coherence is studied by using the small-polaron theory. It is found that in the presence of phonon coupling, the construction of the coherence depends on the state evolution of the phonon bath during the energy exchange. The coherence construction probability is calculated and found to be a decreasing function of both temperature and coupling strength. The spectrum including the twofold decoherence effect is calculated and the obtained result can help one to understand some peculiar observations in the photoluminescence spectrum of a QD TSS system, including the additional cavity line in the strong-coupling regime and the additional coherent scattering in the case of the Mollow triplet. ©2015 American Physical Society.

Yu M.,Jinan University | Ren Q.,Guangzhou University | Yu S.Y.,Guangzhou University
Molecular Biology Reports | Year: 2014

The phosphorylation of nephrin plays an important role in maintaining the normal structure and function in podocytes. Dexamethasone (Dex) is usually used to treat glomerular diseases with proteinuria. In this study, we observated the effect of Dex and angiotensin II (AngII) on the change of nephrin phosphorylation in cultured podocytes. In vitro, cultured podocytes were exposed to AngII (10-6 mol/L) pretreated with or without Dex (100 nM) for different time periods. Nck or Fyn were silenced by small interfering RNA (siRNA), nephrin and its phosphorylation expression were analyzed by Western blotting. In vitro, the phosphorylation of nephrin was significantly reduced after AngII stimulation (P < 0.05). Dex significantly resisted podocyte injury inducted by AngII via increasing the phosphorylation of nephrin (P < 0.05), siRNA silencing Nck can partially inhibited nephrin phosphorylation, siRNA silencing Fyn can completely inhibited nephrin phosphorylation. Phosphorylation of nephrin is important for the survival status of podocytes. Glucocorticoid treatment for human glomerulonephritis may exert its function by regulating Nck and Fyn complex to promote phosphorylation of nephrin. These results elucidate a novel mechanism of glucocorticoid treatment for glomerulonephritis. © 2014 Springer Science+Business Media.

Liu Z.,Hong Kong Polytechnic University | Wu C.,Hong Kong Polytechnic University | Wu C.,Jinan University | Tse M.-L.V.,Hong Kong Polytechnic University | Tam H.-Y.,Hong Kong Polytechnic University
Journal of Lightwave Technology | Year: 2014

In this paper, we report on the fabrication, characterization, and sensing applications of a high-birefringence suspended-core fiber (HB-SCF) using the stack-and-draw technique. Capillaries in two sizes were used for stacking, forming an elliptical core, giving rise to geometric modal birefringence of the HB-SCF. Through a finite-element method, the theoretical values of the phase and group modal birefringence of the HB-SCF were found to be 2.59 \times 10 -4 and 4.75 \times 10-4, respectively. Based on a Sagnac interferometer (SI), the group modal birefringence of the HB-SCF was measured to be 4.84 \times 10-4 , which agrees well with the theoretical result. The sensing characteristics of the HB-SCF SI for pressure, strain, and torsion measurements were analyzed theoretically and investigated experimentally. It exhibits sensitivities of 2.82 nm/MPa for hydrostatic pressure, 0.43 pm/με for strain, and 0.0157/° for torsion measurements. The HB-SCF is made entirely of pure silica without stress-applying parts, thus its modal birefringence is thermally insensitive, resulting in a low temperature sensitivity of ∼1 pm/°C. © 2014 IEEE.

Deng P.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To study the alkaloids from sponge Ircinia sp.. The alkaloids were isolated and purified by various chromatographic techniques, and their structures were elucidated by physicochemical and spectral data. Five alkaloids were isolated and identified as fistularin-1 (1), fistularin-3 (2), aerothionin (3), 11 -hydroxyaerothionin (4), suberamolline A (5). All the alkaloids are isolated from Ircinia sp. for the first time.

Ai B.-Q.,South China Normal University | He Y.-F.,Hebei University | Zhong W.-R.,Jinan University
Soft Matter | Year: 2015

Dynamics and separation of mixed chiral microswimmers are numerically investigated in a channel with regular arrays of rigid half-circle obstacles. For zero shear flow, transport behaviors are the same for different chiral particles: the average velocity decreases with increase of the rotational diffusion coefficient, the direction of the transport can be reversed by tuning the angular velocity, and there exists an optimal value of the packing fraction at which the average velocity takes its maximal value. However, when the shear flow is considered, different chiral particles show different behaviors. By suitably tailoring parameters, particles with different chiralities can move in different directions and can be separated. In addition, we also proposed a space separation method by introducing a constant load, where counterclockwise and clockwise particles stay in different regions of the channel. © The Royal Society of Chemistry 2015.

Wang C.,Jinan University
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] | Year: 2011

To explore the relationship between secular trend of road traffic injuries (RTI) and gross domestic product (GDP) per capita in China. Statistical description was used in the data about cases, injuries, deaths, mileage mortality and 10 million population mortality from 1970 to 2009. Cluster analysis was used to classify the 31 provinces, municipalities and autonomous regions in China. Ecological study was used to explore the relationship between RTI and GDP per capita. There were three stages of RTI in China. It grew rapidly in 1970 - 2002 (from 1.16 to 8.52 per 10 million population), kept steady in 2003 - 2004 (from 8.08 to 8.24 per 10 million population), and decreased obviously in 2005 - 2009 (from 7.55 to 5.08 per 10 million population). The ecological study showed that the population mortality of RTI rose along with the GDP per capita's growth. When the GDP per capita reached to 14 053 yuan (equivalent to 1716 US dollar, in 2005), the mortality began to decrease obviously, the average annual decreasing rate was 10.16%(8.14% - 10.52%)in the following five years. According to the GDP per capita during the period of 1999 - 2009, the 31 provinces, municipalities and autonomous regions in China were divided into three categories of region. The curves of population mortality of RTI and GDP per capita in different category possessed the same ecological trend. That was the population mortality early rose and then fell along with the GDP per capita's growth. All of they started to decrease obviously in 2005. The GDP per capita among three categories of region was different (45 281 yuan, 22 243 yuan and 10 475 yuan respectively) in the same period. In the early stage of economic development, the mortality of RTI increased along with the economic development. When the economic development reached a certain level, the mortality decreased along with the GDP per capita's growth.

Liu C.L.,Jinan University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To study the chemical constituents of roots and stems of Nardostachys chinensis. The chemical constituents were isolated and purified by column chromatography including silica gel, Diaion HP-20, Sephadex LH-20, ODS, and HPLC. Their structures were elucidated on the basis of spectral data analyses. Six compounds were isolated and identified as 8-hydroxypinoresinol-4-O-beta-D-glucopyranoside (1), 8-hydroxypinoresinol-4'-O-beta-D-glucopyranoside (2), 8-hydroxypinoresinol-4-O-(beta-D-glucopyranosyl) 4'-O-beta-D-glucopyranoside (3), cycloolivil 6-O-beta-D-glucopyranoside(4), ferulic acid (5), chlorogenic acid methyl ester (6). All the compounds are isolated from this genus for the first time.

Li X.,Nankai University | Li Y.,Nankai University | Cai X.,Chinese University of Hong Kong | Shan J.,Jinan University
Production and Operations Management | Year: 2016

In recent years, increasing interests have arisen in adding product value through the provision of service. This study considers the problem in which a demand-enhancing service can be provided by different supply chain parties, resulting in four alternative service channels: (a) manufacturer undertaking service, namely M-channel, (b) retailer undertaking service, namely R-channel, (c) manufacturer outsourcing service to third-party (3P), namely M-3P-channel, and (d) retailer outsourcing service to 3P, namely R-3P-channel. We quantitatively model these service channels and derive the optimal decisions with the following observations: When the service costs are equal for the different parties, there is a conflict in service channel choice since both the manufacturer and the retailer prefer to undertake the service or hire the 3P by themselves, although M-channel generates the highest market demand and system profit; When the service costs are different among these parties, R-channel and 3P-channels are more preferable if the service cost is less sensitive to the service level and if the market demand is more sensitive to the service level. We also conduct an empirical study to test some of the insights developed from the analytical models; our empirical findings support the analytical results. © 2015 Production and Operations Management Society.

Thurer M.,Jinan University | Stevenson M.,Lancaster University | Land M.J.,University of Groningen
International Journal of Production Economics | Year: 2016

Workload Control is a production planning and control concept developed for high-variety job shops. It integrates two control mechanisms: (i) input control, to regulate the inflow of work to the system; and (ii) output control, which uses capacity adjustments to regulate the outflow of work from the system. Much Workload Control research has focused on input control, while output control has been largely neglected. Only recently has research emerged that uses Workload Control theory to guide capacity adjustments. Yet this literature focuses on capacity adjustments (output control) only-it fails to integrate it with Workload Control's input control element. In response, this study explores the performance impact of Workload Control when input control (controlled order release) and output control (capacity adjustments) are combined. Job shop simulation results demonstrate that input and output control can and should play complementary roles. Both elements significantly enhance performance in isolation, and performance effects appear to complement each other. Further, results indicate that the choice of the workload threshold that triggers capacity adjustments has a stronger impact on performance than the actual size of the adjustment. The measure of workload used to guide the load-based order release decision is also used to determine the workload threshold that triggers the capacity adjustment. This facilitates implementation in practice. Finally, although our study is on Workload Control, the findings have important implications for other production planning and control concepts. © 2016 Elsevier B.V. All rights reserved.

Wang W.,Jinan University | Hsieh J.P.-A.,Hong Kong Polytechnic University | Song B.,Nanjing University
International Journal of Human-Computer Interaction | Year: 2012

The current article examines user satisfaction with instant messaging in building and maintaining social relationships with friends, family members, and others. The research model integrates motivation theory with media capacity theories to explain how the attributes of media capacity (e.g., social presence and media richness) and users' intrinsic and extrinsic motivations toward using instant messaging influence user satisfaction. Data were collected from a sample of 247 Chinese university students via an online survey. The results suggest that perceived enjoyment, perceived social presence, and perceived usefulness are key to user satisfaction. Perceived social presence and perceived media richness are positively associated with perceived enjoyment. It was also found that perceived enjoyment, perceived social presence, and perceived media richness have significant effects on perceived usefulness. Of interest, perceived enjoyment and perceived social presence have stronger effects on user satisfaction than perceived usefulness. © 2012 Copyright Taylor and Francis Group, LLC.