Guangzhou, China
Guangzhou, China

Jinan University is a public research and comprehensive university based in Guangzhou, Guangdong province, China. It is one of the oldest universities established on mainland China tracing back to the Qing Empire. Its mission is to spread Chinese learning and culture from North to South, and from China overseas. Jinan University was the first university in China to recruit foreign students, and is currently the Chinese university with the largest number of international students.In 1996, it was included in the state's Project 211 for development of key universities. It is a university administered by the Overseas Chinese Affairs Office of the State Council and the Chinese Ministry of Education. Wikipedia.


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Lindsay L.,Oak Ridge National Laboratory | Kuang Y.,Jinan University
Physical Review B - Condensed Matter and Materials Physics | Year: 2017

Intrinsic thermal resistivity critically depends on features of phonon dispersions dictated by harmonic interatomic forces and masses. Here we present the effects of functional group mass variance on vibrational properties and thermal conductivity (κ) of functionalized graphene from first-principles calculations. We use graphane, a buckled graphene backbone with covalently bonded hydrogen atoms on both sides, as the base material and vary the mass of the hydrogen atoms to simulate the effect of mass variance from other functional groups. We find nonmonotonic behavior of κ with increasing mass of the functional group and an unusual crossover from acoustic-dominated to optic-dominated thermal transport behavior. We connect this crossover to changes in the phonon dispersion with varying mass which suppress acoustic phonon velocities, but also give unusually high velocity optic modes. Further, we show that out-of-plane acoustic vibrations contribute significantly more to thermal transport than in-plane acoustic modes despite breaking of a reflection-symmetry-based scattering selection rule responsible for their large contributions in graphene. This work demonstrates the potential for manipulation and engineering of thermal transport properties in two-dimensional materials toward targeted applications. © 2017 American Physical Society.


Xu Z.,Florida State University | Xu Z.,Lawrence Berkeley National Laboratory | Hu B.X.,Florida State University | Hu B.X.,Jinan University
Water Resources Research | Year: 2017

A hybrid discrete-continuum numerical model, Variable-Density Flow and Solute Transport—Conduit Flow Process (VDFST-CFP), is developed to simulate seawater intrusion to a coastal karst aquifer with a conduit network. The Darcy-Weisbach equation is applied to simulate the nonlaminar groundwater flow in the conduit system that is conceptualized as pipes, while the Darcy equation is used for laminar groundwater flow in the continuum porous medium. Density-dependent groundwater flow with appropriate additional density terms in the conduit is analytically derived. The flow and transport equations are coupled, and numerically solved by the finite difference method with an implicit iteration procedure. Two synthetic benchmarks are developed to compare the VDFST-CFP model results with other numerical models, such as the variable-density SEAWAT, constant-density continuum MODFLOW/MT3DMS, and constant-density discrete-continuum CFPv2/UMT3D models. The VDFST-CFP model compares reasonably well with the other model results in both conduit and porous medium domains, and well describes water and salt exchange between the two systems. Under turbulent flow conditions within the conduit, the Darcy-Weisbach equation calculates the flow rate more accurately without overestimation by the Darcy equation. Sensitivity analysis indicates that conduit diameter, friction factor, matrix hydraulic conductivity, and effective medium porosity are important parameters in the VDFST-CFP model. The pros and cons of the VDFST-CFP model are discussed, including the model assumptions and simplifications, limitations of the discrete-continuum modeling method, and the convergence criteria. In general, the newly developed VDFST-CFP model provides a new numerical modeling method for simulating seawater intrusion in a coastal karst aquifer with conduits. © 2016. American Geophysical Union. All Rights Reserved.


Lunagariya J.,Jinan University | Dhar A.,University of Calcutta | Vekariya R.L.,Fuzhou University
RSC Advances | Year: 2017

The efficiency in the catalytic performance of halogen free functionalized room-temperature Brönsted acidic ionic liquids (BAILs), having different acidities (due to the ILs containing zero, a single, and or a double -SO3H functional group), for the esterification of n-butanol with acetic acid under various reaction conditions was investigated. The synthesized BAILs have much weaker corrosiveness than that of H2SO4. The nature of both counter anion and cation as well as the presence of additional functional (-SO3H) groups influenced the behavior of the catalyst. Interestingly, the acidic character of the ILs facilitates the reaction under extremely mild conditions, with a short reaction time, and reduction in the side reactions; moreover, the liquid-liquid biphasic reaction mode leads to good yields. The physicochemical properties of these BAILs were characterized by a variety of different analytical and spectroscopic techniques, such as NMR, FT-IR, mass spectrometry, TGA, and UV-vis spectroscopy for the determination of Hammett acidity. In particular, IL-5 having the highest acidity demonstrated excellent catalytic activity for esterification. An additional advantage of BAILs is the simple procedure for the separation of product and catalyst, where the catalysts can be easily recycled without the loss of catalytic activities, making IL-5 an important alternative catalyst for a commercially viable esterification process. © 2017 The Royal Society of Chemistry.


Chen B.,South China University of Technology | Zou D.,South China University of Technology | Yang Y.,Jinan University
Chemosphere | Year: 2017

Ocean acidification caused by rising CO2 is predicted to increase the concentrations of dissolved species of Fe(II) and Fe(III), leading to the enhanced photosynthetic carbon sequestration in some algal species. In this study, the carbon and nitrogen metabolism in responses to increased iron availability under two CO2 levels (390 μL L−1 and 1000 μL L−1), were investigated in the maricultivated macroalga Pyropia haitanensis (Rhodophyta). The results showed that, elevated CO2 increased soluble carbonhydrate (SC) contents, resulting from enhanced photosynthesis and photosynthetic pigment synthesis in this algae, but declined its soluble protein (SP) contents, resulting in increased ratio of SC/SP. This enhanced photosynthesis performance and carbon accumulation was more significant under iron enrichment condition in seawater, with higher iron uptake rate at high CO2 level. As a key essential biogenic element for algae, Fe-replete functionally contributed to P. haitanensis photosynthesis. Increased SC fundamentally provided carbon skeletons for nitrogen assimilation. The significant increase of carbon and nitrogen assimilation finally contributed to enhanced growth in this alga. This was also intuitively reflected by respiration that provided energy for cellular metabolism and algal growth. We propose that, in the predicted scenario of rising atmospheric CO2, P. haitanensis is capable to adjust its physiology by increasing its carbon and nitrogen metabolism to acclimate the acidified seawater, at the background of global climate change and simultaneously increased iron concentration due to decreased pH levels. © 2017 Elsevier Ltd


Liu Y.,Jinan University | Jiang T.,Fudan University
Journal of Computational and Theoretical Nanoscience | Year: 2016

As the high competitiveness of enterprises depends on the overall quality of staff, how the human resource department designs a set of fair, scientific and rational evaluation system will be directly related to the long-term development of enterprises. Because of the fuzziness of factors in evaluation system, this paper constructs a fuzzy comprehensive evaluation model of performance evaluation. But as fuzzy comprehensive evaluation model is highly subjective, this paper combines RBF neural network model and data envelopment analysis with fuzzy comprehensive evaluation model, to improve the reliability and scientificity of the fuzzy comprehensive evaluation model of performance evaluation, and ensure employees' good development and self-interest. The performance evaluation of human resource not only can combine both qualitative analysis and quantitative analysis, but also can fairly, scientifically and rationally reflect the work of the staff. Meanwhile, this method is simple and clear, has wide application range and flexible operation, and can be perfectly applied and continuously improved in practice. © 2016 American Scientific Publishers All rights reserved.


Liu Y.,Jinan University | Liu M.,Jinan University
Composites Science and Technology | Year: 2017

Polypyrrole-wrapped halloysite nanotubes (PPy@HNTs) are prepared by polymerization of pyrrole on the surfaces of HNTs. PPy@HNTs show improved dispersion ability and stability in water compared with pure PPy due to the increased zeta potential. The PPy@HNTs dispersions are compounded with carboxylated styrene-butadiene rubber (xSBR) latex to prepare conductive xSBR/PPy@HNTs composites. The morphology, conductive performance, mechanical properties, and swelling performance of the xSBR/PPy@HNTs composites are determined. PPy@HNTs can be uniformly dispersed in the rubber matrix and form a conductive network. The conductivity of the composites increases with the loading of PPy@HNTs. When the content of PPy@HNTs is 10%, the conductivity of the xSBR rubber increases to 1.82 × 10−4 s/m which is much higher than the corresponding xSBR/neat PPy composites (4.62 × 10−8 s/m). Also, the composites show significantly improved mechanical properties both in static and dynamic condition. The tensile strength, Young's modulus, and storage modulus of the composites are substantially higher than those of pure xSBR. The rigid filler networks effectively limit the mobility of rubber molecule chains, which leads to decreased water swelling capacity and crosslink density. The prepared high performance rubber composites with good conductivity show promising applications in many areas such as piezoresistive sensor. © 2017 Elsevier Ltd


Chen S.,Jinan University | Tang Y.,Jinan University | Li Z.,Jinan University
ACM International Conference Proceeding Series | Year: 2016

Information technology is one of the most critical tools in higher education, and higher education becomes increasingly dependent on its IT capabilities. With the rapid development of information technologies, university's IT architecture have been changing accordingly. This article presents an improved reference model of university IT architecture named UNITA. UNITA is a general, comprehensive and extensible model, it consists of six layers, including hardware infrastructure, data and information resource, software system, user service, cyber security and IT governance. Compared with the existing model, the improvements of UNITA are: (a) both "service" and "IT governance" are listed separately as a layer in the model, (b) UNITA model reflects the "cloud-pipedevice" structure of the hardware, (c) UNITA model emphasizes the importance of data in higher education institute, (d) UNITA model can be applied to other enterprise industries and can be easily extended to adopt the emerging new technologies. © 2016 ACM.


Ma L.,Jinan University | Fan S.,Jinan University
BMC Bioinformatics | Year: 2017

Background: The random forests algorithm is a type of classifier with prominent universality, a wide application range, and robustness for avoiding overfitting. But there are still some drawbacks to random forests. Therefore, to improve the performance of random forests, this paper seeks to improve imbalanced data processing, feature selection and parameter optimization. Results: We propose the CURE-SMOTE algorithm for the imbalanced data classification problem. Experiments on imbalanced UCI data reveal that the combination of Clustering Using Representatives (CURE) enhances the original synthetic minority oversampling technique (SMOTE) algorithms effectively compared with the classification results on the original data using random sampling, Borderline-SMOTE1, safe-level SMOTE, C-SMOTE, and k-means-SMOTE. Additionally, the hybrid RF (random forests) algorithm has been proposed for feature selection and parameter optimization, which uses the minimum out of bag (OOB) data error as its objective function. Simulation results on binary and higher-dimensional data indicate that the proposed hybrid RF algorithms, hybrid genetic-random forests algorithm, hybrid particle swarm-random forests algorithm and hybrid fish swarm-random forests algorithm can achieve the minimum OOB error and show the best generalization ability. Conclusion: The training set produced from the proposed CURE-SMOTE algorithm is closer to the original data distribution because it contains minimal noise. Thus, better classification results are produced from this feasible and effective algorithm. Moreover, the hybrid algorithm's F-value, G-mean, AUC and OOB scores demonstrate that they surpass the performance of the original RF algorithm. Hence, this hybrid algorithm provides a new way to perform feature selection and parameter optimization. © 2017 The Author(s).


Liu J.,Jinan University | Tang H.,Jinan University
Pan-Pacific Entomologist | Year: 2017

Djalmabatista sinica sp. n. is described and figured based on the adult male and pupa. The male can be separated from its congeners by the characteristic banded wing and the abdominal coloration, while the pupa differs from other species in the setal arrangement on abdominal segment III and the shape of the thoracic horn and the anal lobe. In addition, the pupa of D. reidi (Freeman, 1955) collected in China is briefly redescribed and a new distribution record is given.


Long J.,Jinan University | Wang J.,South China University of Technology | Yu T.,South China University of Technology
Computational intelligence and neuroscience | Year: 2017

The hybrid brain computer interface (BCI) based on motor imagery (MI) and P300 has been a preferred strategy aiming to improve the detection performance through combining the features of each. However, current methods used for combining these two modalities optimize them separately, which does not result in optimal performance. Here, we present an efficient framework to optimize them together by concatenating the features of MI and P300 in a block diagonal form. Then a linear classifier under a dual spectral norm regularizer is applied to the combined features. Under this framework, the hybrid features of MI and P300 can be learned, selected, and combined together directly. Experimental results on the data set of hybrid BCI based on MI and P300 are provided to illustrate competitive performance of the proposed method against other conventional methods. This provides an evidence that the method used here contributes to the discrimination performance of the brain state in hybrid BCI.


Huang Y.,Jinan University | Yu B.,Jinan University | Guo T.,Jinan University | Guan B.-O.,Jinan University
RSC Advances | Year: 2017

Ultrasensitive and in situ DNA detection at different pH values, ranging from 4.3 to 8.5, based on a microfiber with a graphene oxide linking layer was proposed and experimentally demonstrated. The graphene oxide coating over the silica microfiber provides a strong π-π interaction with the detected single-stranded DNA molecules, and induces an amplified surface refractive index modulation over the fiber, and finally leads to a wavelength shift of the optical interference fringe for interrogation. Using this strategy, in situ DNA measurement with a detection limit of up to 10−12 M and a linear response in the pH range from 4.3-8.5 has been achieved. Benefiting from its compact size, high sensitivity, and ease of use, together with remote operational ability, the proposed sensor opens up a multitude of opportunities for quantifying DNA in various hard-to-reach environments. It may supplement the existing DNA detection tools. © The Royal Society of Chemistry.


Six ferrous chloride complexes ((Ar-BIANX)FeCl2: Ar = 2,6-diisopropylphenyl (Dipp), X = F (1), Cl (2), Me (3); Ar = mesityl (Mes), X = F (4), Cl (5), Me (6)) supported by sterically bulky asymmetric bis(arylimino)acenaphthene (BIAN) ligands were prepared through the treatment of anhydrous FeCl2 with the corresponding ligands in a molar ratio of 1:1. The compounds were characterized by X-ray crystallography, IR, NMR and electrochemical methods. This series of complexes represents rare examples of structurally characterized iron compounds bearing asymmetric bidentate BIAN ligands. The complexes were tested for catalytic hydrosilylation of aldehydes and ketones at room temperature, and moderate to good yields of alcohol derivatives were obtained after hydrolysis workup. © The Royal Society of Chemistry.


Cao Y.M.,South-Central University for Nationalities | Tang H.Q.,Jinan University
Zoological Studies | Year: 2017

Three species of the genus Zavreliella from East Asia are reviewed in this paper, including a new species, Z. shidai sp. n., based on associated pupae and males collected from Guangdong Province, China. The new species can be separated from other congeners by the uniquely banded wing and marked abdomen. The Japanese species Z. inawaheia Sasa, Kitami and Suzuki, 2001 is re-described based on the holotype. In addition, two additional records are added for Z. marmorata (v. d. Wulp). An emended generic diagnosis and keys to the known males from Asia are also provided. © 2017, Academia Sinica. All rights reserved.


Li X.,Jinan University | Yin P.,Jinan University | Zhao L.,Jinan University
Food and Chemical Toxicology | Year: 2017

Bisphenol A, dibutyl phthalate and cadmium can be found in environment simultaneously. Several studies suggested that they had genotoxic effect. In this study, mono-exposure and co-exposure treatments, designed by 3 × 3 full factorial, were established to determine the individual toxicity and binary mixtures’ combined effects on the oxidative stress and genotoxicity in HepG 2 cells. The highest oxidative damage was observed in the Cd treatments groups. Compared with control groups, the maximum level of reactive oxygen species and malondialdehyde were ∼1.4 fold and ∼2.22 fold respectively. And a minimum level of superoxide dismutase activity was found with the decrease of 43%. The mechanism that excessive oxidative stress led to the DNA damage was inferred. However, cells treated with BPA showed the worst DNA damage rather than Cd, which may because Cd mainly damages DNA repairing mechanism. For the joint effect, different interactions can be found in different biological endpoints for different combinations since different mechanisms have been clarified in mixture toxicity studies. It is sure that the co-exposure groups enhanced cytotoxicity, oxidative stress and genotoxicity compared to the mono-exposures. Synergistic and additive interactions were considered, which means greater threat to organisms when exposed to multiple estrogenic endocrine disruptors. © 2017 Elsevier Ltd


Qiu C.,Jinan University | Wang B.,South China University of Technology | Wang Y.,Jinan University | Teng Y.,Jinan University
Food Hydrocolloids | Year: 2017

Deamidated wheat gliadin (gliadin) and its conjugates were applied to form complexes with resveratrol (Res). Res is a kind of polyphenol known for its low bioavailability and solubility in aqueous solution. The morphology of the complexes and Res stability were compared. Colloidal complexes were incorporated into emulsion to evaluate antioxidant activity. Results indicated that both gliadin and glycosylated gliadin can form nanocomplexes with Res and had high retention efficiency. Colloidal complexes solution can efficiently protect Res from UV-light transformation and the content of trans-Res was significantly improved (p < 0.05). Higher Res bioaccessibility in the complexes after digestion indicated that colloidal complexes can efficiently increase Res stability during gastrointestinal digestion. Dextran moieties were more efficient to enhance Res bioaccessibility due to steric hindrance. In the emulsion system, the colloidal complexes can significantly lower lipid oxidation evaluated by lipid hydroperoxides and hexanal generation during storage. The emulsifying properties of protein promoted the accumulation of Res at the oil-water surface. Therefore, it can act as an antioxidant to inhibit lipid oxidation. These results proved that the gliadin based delivery system is efficient to incorporate bioactive compounds in food beverages and emulsions. © 2017 Elsevier Ltd


Luo X.,Jinan University | Yu Y.,Jinan University | Xiang Z.,Jinan University | Wu H.,Jinan University | And 5 more authors.
Journal of Neurochemistry | Year: 2017

Adding a free radical-scavenging nitrone moiety on tetramethylpyrazine, we have previously synthesized a chemical named 2-[[(1,1-dimethylethyl)oxidoimino]-methyl]-3,5,6-trimethylpyrazine (tetramethylpyrazine nitrone, or TBN) and proved its neuroprotective effect but with limited understanding of its mechanism. Here we ask if TBN protects retinal ganglion cells (RGCs) against excitotoxicity induced by NMDA and explore the underlying mechanism. NMDA was intravitreally injected to induce RGC injury in rats, followed by daily intraperitoneal administrations of TBN. Measurements of TBN concentration at different times after intraperitoneal administration showed that more than 200 μM TBN reached the aqueous humor quickly. Then RGCs' survival was evaluated by quantifying Brn3-positive cells, and retinal functions were examined by electroretinogram and visual behaviors. TBN significantly increased the survival of RGCs after NMDA insult, recovered the amplitude of photopic negative responses to flash, and restored the visual behavior. Furthermore, TBN inhibited the apoptotic process, as indicated by the elevated ratios of cleaved caspase-3/caspase-3 and of Bax/Bcl-2, and decreased the level of reactive oxygen species. Moreover, TBN reduced RGC's calcium overload induced by NMDA or by KCl. Whole-cell patch recording from RGCs further showed that TBN slightly but significantly inhibited L-type calcium channels, but had little effect on T-type calcium channel or NMDA-, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid(AMPA)-induced current. Thus our data indicate that TBN alleviates NMDA-elicited injury of rat RGCs both morphologically and functionally, possibly by inhibiting the L-type calcium channel thus reducing Ca2+ overload and by directly scavenging free radicals. Therefore, TBN may be a novel candidate for treating excitotoxicity-related visual disorders such as glaucoma. © 2017 International Society for Neurochemistry.


Wang F.,Jinan University | Zhang Q.,Jinan University
North American Journal of Aquaculture | Year: 2017

Saprolegniasis, a fungal fish disease, is an important challenge in the aquaculture industry. To find a natural agent to control saprolegniasis, an anti-Saprolegnia bacterium, named XL03, was isolated from the sediment of the Baimang River in Xili, Guangzhou, China. The bacterium was identified as Pseudomonas protegens by means of 16S rDNA gene sequence and its fluorescent character. Saprolegnia sp. was used to evaluate the antifungal activity of the P. protegens strain XL03. The cell-free disruption supernatant (CFDS) showed an antifungal zone of 19.34 ± 1.20 mm against the Saprolegnia hyphae on the potato dextrose agar (20% potato extract, 2% glucose, 2% agar) medium plate and depicted a minimum inhibitory concentration (MIC = 12.5 mg/mL) against the Saprolegnia spore germination in vitro. To purify the antifungal substance from the CFDS, ammonium sulphate precipitation, gel filtration, and ion-exchange chromatography were applied, and the purified peptide exhibited a single band at molecular weight about 58 kDa on a SDS-PAGE gels. Furthermore, the MIC of the purified peptide was identified as 0.0625 mg/mL against Saprolegnia spores, and the activity of peptide showed thermal stability between 30°C and 60°C but retained activity only in a narrow pH range of 5.0–9.0. These findings demonstrate that P. protegens strain XL03 has the potential to be used in biocontrol of saprolegniasis in aquaculture. Received June 21, 2016; accepted October 24, 2016 Published online February 23, 2017 © American Fisheries Society 2017.


Two interesting species, Conchapelopia togamaculosa Sasa & Okazawa and a new species, Conchapelopia brachiata sp. n., were collected from southern China. The male, pupa and larva of the new species are described, and new distributions of the former species are noted. Although the male of the new species is very distinct from that of the former in the hypopygial median volsella, the pupa and larva stunningly resemble those of the former. © 2017 Magnolia Press.


Chen F.-P.,South China University of Technology | Ou S.-Y.,Jinan University | Chen Z.,South China University of Technology | Tang C.-H.,South China University of Technology
Journal of Agricultural and Food Chemistry | Year: 2017

The complexation between soy soluble polysaccharide (SSPS) and curcumin at pH 7.0 and 4.0, as well as some physicochemical characteristics of the resultant complexes, was investigated. The encapsulation efficiency and loading amount of curcumin in the complexes at pH 4.0 reached 67.3% and 4.49 μg/mg SSPS, respectively. Ethanol-induced denaturation and structural unfolding of the protein fraction in SSPS was essential for complex formation. The complexation with curcumin resulted in aggregation of SSPS and the subsequent formation of compacted nanoparticles with curcumin as the core. The complexation greatly improved the heat stability and in vitro bioaccessibility of curcumin. In general, the encapsulation efficiency, heat stability, and bioaccessibility of curcumin in the complexes at pH 4.0 were better than those at pH 7.0. The findings are of importance for the development of food grade nanovehicles for enhanced water solubility, stability, and bioaccessibility of hydrophobic bioactives. © 2017 American Chemical Society.


Zhong M.,Jinan University | Yin P.,Jinan University | Zhao L.,Jinan University
Environmental Science and Pollution Research | Year: 2017

The objective of the present work was to evaluate the toxic effect of nonylphenol (NP) on the antioxidant response and antitumor activity of Gracilaria lemaneiformis. An obvious oxidative damage was observed in this study. The thallus exposed to NP showed 1.2–2.0-fold increase in lipid peroxide and displayed a maximum level of 16.58 μmol g−1 Fw on 0.6 mg L−1 for 15-day exposure. The activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) enhanced significantly by 1.1–3.2-fold and subsequently diminished at the high concentrations and prolonged exposure. The results of DNA damage in comet assay also supported that NP was obviously toxic on G. lemaneiformis with increasing the percentage of tail DNA in a dose-dependent manner. Furthermore, the ethanol extract of G. lemaneiformis (EEGL) did exhibit antitumor potential against HepG-2 cells. While decreased in cell inhibition, ROS generation, apoptosis, and caspase-3 in HepG-2 cells treated with the EEGL were observed when G. lemaneiformis was exposed to NP for 15 days, and which were related to exposure concentration of NP. These suggested that NP has strongly toxic effect on the antitumor activity of G. lemaneiformis. The results revealed in this study imply that macroalgae can be useful biomarkers to evaluate marine pollutions. © 2017 Springer-Verlag Berlin Heidelberg


Zhou B.,Jinan University | Qiu Y.,Jinan University | Wen Q.,Jinan University | Zhu M.,Jinan University | Yang P.,Jinan University
ACS Applied Materials and Interfaces | Year: 2017

(Figure Presented) A mutiplex cytosensor based on a dual electrochemiluminescence (ECL) signal system was fabricated for in situ and simultaneous detection of the expression levels of multiple cell-surface receptors, mannose and epidermal growth factor receptor (EGFR), using luminol-capped gold nanoparticles (Au@luminol) and CdS quantum dots (CdS QDs) as potential-resolved ECL nanoprobes. Two spatially resolved areas on indium tin oxide (ITO) electrodes were modified with polyaniline (PANI) by electropolymerization, on which gold nanoparticles (AuNPs) were attached to strengthen conductivity and stability of the sensing interface. Human mucin1 protein (MUC1) aptamer was immobilized onto AuNPs for capturing MUC1-positive MCF-7 cells. Au@luminol and CdS QDs as ECL nanoprobes were covalently linked with concanavalin A (ConA) and epidermal growth factor (EGF) to label MCF-7 cells on the two areas of the cytosensor separately. Compared to conventional multiplex biosensor, we demonstrated a novel analysis platform for the simultaneous detection of multiple cell-surface receptors; it could provide two sensitive and potential-resolved ECL signals during one potential scanning and avoid cross-reactivity between the two nanoprobes. The quantification of MCF-7 cells on the two spatially resolved areas could be achieved over the linear range from 102 to 1.0 × 106 cells mL-1 with a detection limit of 20 cells mL-1. This multiplex cytosensor was further applied for simultaneous quantitative evaluation of the expression levels of mannose and EGFR on MCF-7 cells, revealed that the average numbers of mannose and EGFR per captured MCF-7 cell were 1.2 × 106 and 0.86 × 105 with the relative standard deviation of 5.3% and 4.2%, respectively. The multiplex cytosensor was capable of evaluating multiple cell-surface receptors, which would be beneficial to developing a better diagnostic tool for diseases. © 2016 American Chemical Society.


Ou J.,Jinan University | Zhong X.,Guangdong University of Finance | Qi X.,Hong Kong University of Science and Technology
Naval Research Logistics | Year: 2016

In this article, we study a parallel machine scheduling problem with inclusive processing set restrictions and the option of job rejection. In the problem, each job is compatible to a subset of machines, and machines are linearly ordered such that a higher-indexed machine can process all those jobs that a lower-indexed machine can process (but not conversely). To achieve a tight production due date, some of the jobs might be rejected at certain penalty. We first study the problem of minimizing the makespan of all accepted jobs plus the total penalty cost of all rejected jobs, where we develop a (5/3+ ε)- approximation algorithm with a time complexity of O(nm/ε2 · log P). We then study two bicriteria variants of the problem. For the variant problem of minimizing the makespan subject to a given bound on the total rejection cost, we develop a (5/3+ ε) -approximation algorithm with a time complexity of O(n2/ε2). For the variant problem of maximizing the total rejection cost of the accepted jobs subject to a given bound on the makespan, we present a 0.5-approximation algorithm with a time complexity of O(n log n). © 2016 Wiley Periodicals, Inc. Naval Research Logistics 63: 667–681, 2017. © 2017 Wiley Periodicals, Inc.


Liang L.,Jinan University | Jin L.,Jinan University | Guan B.-O.,Jinan University
2016 Conference on Lasers and Electro-Optics, CLEO 2016 | Year: 2016

A wavelength-encoded optofluidic sensor is fabricated by aligning a tapered optical fiber in lateral contact with a liquid-filled silica capillary. Preliminary experiment demonstrates its capability of molecular microRNA quantification with detection limit less than 2nM. © 2016 OSA.


Zhan J.,Jinan University | Yu L.,Jinan University
Zhendong yu Chongji/Journal of Vibration and Shock | Year: 2017

Here, a novel effective independence-improved modal strain energy (EI-IMSE) method was proposed to improve the effective independence (EI) algorithm in structural health monitoring field. The EI-IMSE optimal sensor placement (OSP) method was verified using a numerical simulation for a 3D truss tower and a test for a rectangular hollow steel beam hinged at both ends. Based on four criteria, the EI-IMSE OSP method was evaluated compared with the EI method, the EI-driving point residue (EI-DPR) one, the EI-average driving DOF velocity (EI-ADDOFV) one, and the EI-average acceleration amplitude (EI-AAA) one. The results showed that the proposed EI-IMSE method can not only retain advantages of the EI method but also have a better accuracy in identifying low order modes with a good robustness. © 2017, Editorial Office of Journal of Vibration and Shock. All right reserved.


Yang Q.,Jinan University | Yang Q.,Huazhong University of Science and Technology | Di H.,Huazhong University of Science and Technology
International Journal of Modern Physics A | Year: 2017

If axion-like particles (ALPs) constitute a major part of dark matter (DM), due to their bosonic nature and a relatively small mass, they could behave differently from the pointlike dark matter particles on the formation of the cosmic structures. When studying the structure formation, it is often useful to consider DM as a special fluid with a given density and a given velocity. ALP fluid obeys the same continuity equation compared to the pointlike collisionless DM, but has a different first-order velocity equation. In the linear regime of structure formation, the resulted observational differences are negligible for the QCD axions but can be interesting for very light ALPs. © 2017 World Scientific Publishing Company.


Cheng Y.,Jinan University
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2016

Alagille syndrome (ALGS) is an autosomal dominant disorder which is mainly caused by JAG1 gene mutation and can affect multiple systems including the liver, heart, eyes, skeleton and face. This paper reports the clinical and genetic features of an ALGS patient. A 2-year-and-9-month-old boy was referred to the hospital with the complaint of abnormal liver function and heart murmur discovered over two years. Jaundice of the skin and sclera was not observed. The child had a prominent forehead, left esotropia, depressed nasal bridge and micromandible. The two lungs were clear on auscultation, but a systolic cardiac murmur of grade 2/6 could be heard between the 2nd and 3rd intercostal space at the left sternal border. Neither abdominal distension nor enlarged liver or spleen was discovered. X-ray radiography uncovered butterfly malformation of the 6th and 8th thoracic vertebrae. Serum biochemistry analysis revealed elevation of total bile acids, bilirubin and transaminases. Based on the clinical characteristics and the consultation opinion of the ophthalmologist, the child was diagnosed to have ALGS with Duane retraction syndrome. DNA direct sequencing detected a novel JAG1 mutation c.2419delG(p.Glu807AsnfsX819) in the child. Symptomatic and supportive therapy was performed thereafter and clinical follow-up was conducted until he was 4 years and 2 months. In the follow-up visits, his general condition remained stable, but the facial malformations, left esotropia, cardiac murmur and abnormal liver function persistend. The long-term outcome needed to be observed.


Qiu S.,Jinan University | Liu J.,Jinan University | Xing F.,Jinan University
Cancer Science | Year: 2017

Polyamines are multivalent and organic cations essential for cellular growth, proliferation, differentiation, and apoptosis. Increased levels of polyamines are closely associated with numerous forms of cancer. An autoregulatory circuit composed of ornithine decarboxylase (ODC), antizyme (AZ) and antizyme inhibitor (AZI) govern the intracellular level of polyamines. Antizyme binds with ODC to inhibit ODC activity and to promote the ubiquitin-independent degradation of ODC. Antizyme inhibitor binds to AZ with a higher affinity than ODC. Consequently, ODC is released from the ODC–AZ complex to rescue its activity. Antizyme inhibitor increases the ODC activity to accelerate the formation of intracellular polyamines, triggering gastric and breast carcinogenesis as well as hepatocellular carcinoma and esophageal squamous cell carcinoma development. Antizyme inhibitor 1 (AZIN1), a primary member of the AZI family, has aroused more attention because of its contribution to cancer. Even though its conformation is changed by adenosine-to-inosine (A→I) RNA editing, it plays an important role in tumorigenesis through regulating intracellular polyamines. Encouragingly, AZIN1 has been revealed to have an additional function outside the polyamine pathway so as to bypass the deficiency of targeting the polyamine biosynthetic pathway, promising to become a critical target for cancer therapy. Here, we review the latest research advances into AZIN1 and its potential contribution to carcinogenesis. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.


Li J.,Jinan University | Wang R.,Jinan University | Huang S.-G.,Jinan University
American Journal of Translational Research | Year: 2017

This study aims to observe expression of IL-27 on different cells in periapical tissues of different types of human chronic periapical diseases. Periapical tissue specimens of 60 donors, including healthy control (n=20), periapical granuloma group (n=20) and radicular cysts group (n=20), were fixed in 10% buffered formalin, stained with hematoxylin and eosin for histopathology. Then specimens were stained with double- immuno-fluorescence assay for identification of IL-27-tryptase (mast cells, MCs), IL-27-CD14 (mononuclear phagocyte cells, MPs) and IL-27-CD31 (endothelial cells, ECs) double-positive cells in periapical tissues. The results indicated that compared with healthy control, the densities (cells/mm2) of IL-27-tryptase, IL-27-CD14 and IL-27-CD31 double-positive cells were significantly increased in human chronic periapical diseases (periapical granuloma group and radicular cysts group) (P<0.001). The density of IL-27-tryptase double positive cells in radicular cysts group was significantly higher than those in periapical granuloma group (P<0.001). Densities of IL-27-CD14 and IL-27-CD31 double-positive cells in periapical granuloma group had no significant difference with those in radicular cysts group (P=0.170 and 0.138, respectively). IL-27-CD14 double positive cells density achieved to peak among three cell groups in radicular cysts groups. In conclusion, IL-27 expressed in MCs, MPs and ECs of human chronic periapical diseases with different degrees. IL-27-tryptase double-positive cells may participate in pathogenic mechanism of chronic periapical diseases, especially for formation of fibrous in periapical cysts. IL-27-CD14 and IL-27-CD31 double-positive cells may participate in immunologic response to resist periapical infection, and they may play an dual role in pathogenesis and localization of periapical diseases. © 2017, E-Century Publishing Corporation. All rights reserved.


Feng F.-R.,Jinan University | Ran Y.,Jinan University | Guan B.-O.,Jinan University
Optics InfoBase Conference Papers | Year: 2016

An abnormal phenomenon of secondary dip existing in type In Bragg grating formation, which is highly dependent on the diameter and photosensitivity of the fiber core, is discovered and investigated. © OSA 2016.


Fang J.,Jinan University | Deng W.,Jinan University | Ma X.,Jinan University | Huang J.,Jinan University | Wu W.,South China University of Technology
IEEE Electron Device Letters | Year: 2017

In this letter, for both non-degenerate conduction and degenerate conduction, a new compact model of amorphous oxide semiconductor thin-film transistors is developed. The contributions of the trapped and free charges are considered in the closed-form solutions of surface potential and drain current under a degenerate regime. Furthermore, proof of the model's accuracy has been obtained by comparisons with numerical results and measured data. The proposed scheme is capable of capturing real-device dc characteristics and of maintaining high computational efficiency, which provides a better platform to develop advanced surface-potential-based model for circuit simulation. © 1980-2012 IEEE.


Guan B.-O.,Jinan University | Sun L.-P.,Jinan University | Huang Y.,Jinan University
Optics InfoBase Conference Papers | Year: 2016

In this paper, the basic principles and characteristics of optical microfiber mode interferometer are introduced, and the recent progress in their applications as chemical and biological sensors are reviewed. © OSA 2016.


Feng F.-R.,Jinan University | Ran Y.,Jinan University | Guan B.-O.,Jinan University
Optics InfoBase Conference Papers | Year: 2016

An abnormal phenomenon of secondary dip existing in type In Bragg grating formation is discovered and the laser applications based on those secondary-type In gratings are researched. © OSA 2016.


Xiao J.,Jinan University | Xiao J.,Shenzhen Third Peoples Hospital | Xiao J.,University of Hong Kong | Tipoe G.L.,University of Hong Kong
Frontiers in Bioscience - Landmark | Year: 2016

Non-alcoholic fatty liver disease (NAFLD) is a leading liver disorder in the world. Inflammation is one of the most important pathological events during the development of NAFLD and also represents the hallmark between simple steatosis and non-alcoholic steatohepatitis (NASH). Inflammasomes are novel protein complex platforms assembled in response to patternassociated molecular patterns (PAMPs) and damageassociated molecular patterns (DAMPs). Currently, there are several identified inflammasomes, including nod-like receptor protein (NLRP)-1, 2, 3, 6, 10, 12, NLRC4 and absent in melanoma 2 (AIM2) inflammasomes. In the liver, inflammasomes are primarily expressed in immune cells. However, increasing evidence suggests that their expressions in other types of cells in the liver are also present. In general, inflammasomes are up-regulated in various liver diseases. In NAFLD, it is reported that the levels of inflammasome components (e.g. NLRPs, caspase-1, IL-1β and IL-18) are elevated. Silence of these components attenuates hepatic injury. Collectively, the main purposes of this review are to examine the recent progress of hepatic inflammasome research and to discuss possible directions of therapeutic strategy and development against NAFLD.


Zheng J.,Jinan University | Cheng F.,Jinan University | Yang L.,Jinan University
Jisuanji Jicheng Zhizao Xitong/Computer Integrated Manufacturing Systems, CIMS | Year: 2017

To solve the jointed decisions problem of ordering, pricing and old products disposing faced for selling perishable products with a multi-period shelf life over an infinite horizon effectively, a model with Markov decision theory was established and the optimal policy was computed by using Q-learning algorithm. The optimal policy indicated the action of all states which could maximize the long-run discounted expected profit from current period. Through interacting with the environment and obtaining the feedback continuously, the iterate formula of algorithm renewed the optimal policy constantly. The stationary optimal policy would be computed after sufficient learning under situation of state and action space were finite and discrete, while the state transition probability and expected profit were not necessarily be known. The research showed that the different parameters had different and significant impact on the characteristic of each decision, and the conclusion provided some support and thought for researches of heuristic strategy. © 2017, Editorial Department of CIMS. All right reserved.


Cheng Q.,Jinan University | Yi H.,Ohio State University
Renewable and Sustainable Energy Reviews | Year: 2017

This paper provides an extensive review of two streams of literature: the first part of the review focuses on policy tools and their interrelationships and the second part focuses on the literature on the state renewable energy policy tools. Based on the reviews, this paper investigates how policymakers choose a set of interrelated renewable energy policy instruments and identify under what conditions policy instruments complement or substitute. We extend the political market framework by examining the influences of: (1) administrative agencies on the supply side; (2) interest groups on the demand side; and (3) the policy-induced problem situation changes on the adoption of state renewable energy policy tools, building upon reviews of both policy tools and policy diffusion theories. A set of hypotheses are advanced for state renewable energy policy interactions among public benefit fund (PBF), renewable portfolio standards (RPS) and corporate tax incentive (CTI). The hypotheses are tested by three Event History models, in which RPS, PBF and CTI serve as dependent variables separately. The complementary effects between previous use of RPS and adoption of PBF, between previous use of PBF and adoption of RPS, and between previous use of CTI and adoption of RPS are confirmed. We also find support for the policy substitutability between previous use of CTI and adoption of PBF. © 2016 Elsevier Ltd


Huang S.-Q.,Jinan University | Zhang Z.,Jinan University | Li Y.,Jinan University | Liu Z.-S.,Guangdong University of Technology | Li Y.-H.,University of Sydney
Information Sciences | Year: 2017

In multi-hop wireless networks, the maximum number of hops between clients and the service gateway (GW) significantly impacts the quality of network service, and thus GW deployment optimization plays a critical role in network design and planning. It is well know that the optimal GW deployment can be formulated as a vertex k-centre problem. However, achieving the optimal solution of a k-centre problem is highly complex due to its large solution space. To address this issue we propose a new algorithm based on the substitution principle of network by exploring the inclusion relationship of adjacent node subsets, to reduce the original network to a smaller scale substitution graph. The proposed algorithm can eliminate a large number of redundant nodes, thus reducing the solution space of the optimization problem, and improving the probability of achieving a globally optimal solution. The performance of the proposed algorithm is also analysed. Simulation results show that the proposed t-step substitution algorithm can significantly reduce the solution space of the k-centre problem by up to 80%. We then apply the proposed algorithms to traditional optimization methods, such as genetic (GA), artificial immune (AIA), and K-means for solving discrete space problems, and it is shown that the substitution based algorithm can significantly improve the performance of respective traditional GA, AIA and K-means methods,yielding a better GW deployment scheme with a smaller covering radius. © 2017 Elsevier Inc.


Zheng L.M.,Jinan University | Zhang S.X.,Jinan University | Tang K.S.,City University of Hong Kong | Zheng S.Y.,Sun Yat Sen University
Information Sciences | Year: 2017

Differential evolution (DE) algorithms have demonstrated excellence performance in dealing with global optimization problems. In DE, mutation is the sole process providing new components to form potential candidates, and it does so by combining various existing solution vectors. In the past two decades, many mutation strategies have been proposed with the goal of achieving better searching capability. Commonly, the best candidate in the current population or its subset is employed. In this study, we challenge the approach of adopting only the single best vector and suggest enhancing DE with the collective information of the m best candidates. The evolutionary information of these m best candidates is linearly combined to form a part of the difference vector in mutation. Moreover, the collective information can also be used in crossover. Consequently, a new DE variant called collective information-powered differential evolution (CIPDE) is constructed. To verify its effectiveness, CIPDE is compared with seven state-of-the-art DE variants on 28 CEC2013 benchmark functions. Numerical results confirm that CIPDE is superior to the other DEs for most of the test functions. The impacts of the components of CIPDE and performance sensitivities to system parameters are also investigated. © 2017 Elsevier Inc.


Yu X.,Jinan University | Yu X.,Hong Kong Polytechnic University | Yao Z.-P.,Hong Kong Polytechnic University | Yao Z.-P.,Yanbian University
Analytica Chimica Acta | Year: 2017

Chiral analysis is of great importance to fundamental and applied research in chemical, biological and pharmaceutical sciences. Due to the superiority of mass spectrometry (MS) over other analytical methods in terms of speed, specificity and sensitivity, chiral analysis by MS has attracted much interest in recent years. Chiral analysis by MS typically involves introduction of a chiral selector to form diastereomers with analyte enantiomers, and comparison of the behaviors of diastereomers in MS. Chiral differentiation can be achieved by comparing the relative abundances of diastereomers, the thermodynamic or kinetic constants of ion-molecule reactions of diastereomers in the gas phase, the dissociation of diastereomers in MS/MS, or the mobility of diastereomers in ion mobility mass spectrometry. In this review, chiral recognition and determination of enantiomeric excess by these chiral MS methods were summarized, and the prospects of chiral analysis by MS were discussed. © 2017 Elsevier B.V.


Wu Y.Y.,Jinan University | Meng M.,Jinan University | Wang G.Y.,Jinan University | Feng P.,Jinan University | Liu C.Y.,Jinan University
Chemical Communications | Year: 2017

Four thienylene (C4H2S) bridged Mo2 dimers, [Mo2(DAniF)3]2(μ-OOCC4H2SCOO) (DAniF = N,N′-di(p-anisyl)formamidinate), [Mo2(DAniF)3]2(μ-N(H)SCC4H2SCN(H)S), [Mo2(DAniF)3]2(μ-OSCC4H2SCSO) and [Mo2(DAniF)3]2(μ-SSCC4H2SCSS), have been synthesized and studied in terms of electronic coupling. The subtle structural differences between these compounds vary largely the extent of electron delocalization; consequently, a systematic transition from Class II to Class III via Class II-III is achieved, which is probed using spectral parameters of intervalence charge transfer (IVCT) absorption (band energy, intensity and shape) for the mixed-valence complexes. Significantly, the species in Class II-III displays a low energy, half cut-off and solvent-dependent IVCT band, while a high energy, less asymmetrical IVCT band is observed for the complex in Class III. These results give fresh and detailed understanding of the system transition. © The Royal Society of Chemistry.


Huang S.,Jinan University | Tao M.,Dongguan University of Technology
Sensors (Switzerland) | Year: 2017

Wireless sensor network topology optimization is a highly important issue, and topology control through node selection can improve the efficiency of data forwarding, while saving energy and prolonging lifetime of the network. To address the problem of connecting a wireless sensor network to the Internet in cyber-physical systems, here we propose a geometric gateway deployment based on a competitive swarm optimizer algorithm. The particle swarm optimization (PSO) algorithm has a continuous search feature in the solution space, which makes it suitable for finding the geometric center of gateway deployment; however, its search mechanism is limited to the individual optimum (pbest) and the population optimum (gbest); thus, it easily falls into local optima. In order to improve the particle search mechanism and enhance the search efficiency of the algorithm, we introduce a new competitive swarm optimizer (CSO) algorithm. The CSO search algorithm is based on an inter-particle competition mechanism and can effectively avoid trapping of the population falling into a local optimum. With the improvement of an adaptive opposition-based search and its ability to dynamically parameter adjustments, this algorithm can maintain the diversity of the entire swarm to solve geometric K-center gateway deployment problems. The simulation results show that this CSO algorithm has a good global explorative ability as well as convergence speed and can improve the network quality of service (QoS) level of cyber-physical systems by obtaining a minimum network coverage radius. We also find that the CSO algorithm is more stable, robust and effective in solving the problem of geometric gateway deployment as compared to the PSO or Kmedoids algorithms. © 2017 by the authors; licensee MDPI, Basel, Switzerland.


Jin B.,Sun Yat Sen University | Wang C.,Sun Yat Sen University | Li J.,Sun Yat Sen University | Du X.,Guangdong General Hospital Guangdong Academy of Medical science | And 3 more authors.
Clinical Cancer Research | Year: 2017

Purpose: Leukemia stem cells (LSC), which are insensitive to tyrosine kinase inhibitors (TKI), are an important source of TKI resistance and disease relapse in chronic myelogenous leukemia (CML). Obstacles to eradicating LSCs include limited understanding of the regulation network of LSCs. The current study aimed to examine the interplay between NF-κB and FOXM1/β-catenin, and the effect of its chemical intervention on CML LSCs. Experimental Design: The interplay between NF-κB and FOXM1/β-catenin was analyzed by reciprocal coimmunoprecipitation (co-IP) and chromatin immunoprecipitation (ChIP) assay in CML cells. The effect of disturbing NF-κB and FOXM1/β-catenin by niclosamide on the self-renewal capacity and survival of LSCs was evaluated in vitro in human primary CML CD34+ cells and in vivo in CML mice. Results: Reciprocal co-IP experiments showed physical interaction of p65 and FOXM1. p65 promoted transcription of FOXM1 gene. ChIP assay revealed recruitment of p65 on the promoter of FOXM1 gene. Conversely, FOXM1 and β-catenin positively regulated the nuclear translocation and transcriptional activity of NF-κB in CML cells. Niclosamide disrupted the positive feedback loop between NF-κB and FOXM1/β-catenin, thereby impairing the self-renewal capacity and survival of CML LSCs. Niclosamide decreased the long-term engraftment of human CML LSCs in NOD-SCID IL2Rg chain-deficient (NOG) mice, and prolonged the survival of CML mice. Conclusions: Interaction of p65 with FOXM1/β-catenin is critical in CML and its disruption by niclosamide eradicates LSCs. These findings may improve the understanding of a self-renewal regulatory mechanism of LSCs and offer a rationale-based approach to eliminate LSCs in CML. ©2016 AACR.


CHEN S.-J.,Sun Yat Sen University | ZHANG Y.-X.,Sun Yat Sen University | HUANG S.-G.,Jinan University | LU F.-L.,Sun Yat Sen University
Parasitology | Year: 2017

Ocular toxoplasmosis (OT) caused by Toxoplasma gondii is a major cause of infectious uveitis, however little is known about its immunopathological mechanism. Susceptible C57BL/6 (B6) and resistant BALB/c mice were intravitreally infected with 500 tachyzoites of the RH strain of T. gondii. B6 mice showed more severe ocular pathology and higher parasite loads in the eyes. The levels of galectin (Gal)-9 and its receptors (Tim-3 and CD137), interferon (IFN)-γ, IL-6 and IL-10 were significantly higher in the eyes of B6 mice than those of BALB/c mice; however, the levels of IFN-α and -β were significantly decreased in the eyes and CLNs of B6 mice but significantly increased in BALB/c mice after infection. After blockage of galectin–receptor interactions by α-lactose, neither ocular immunopathology nor parasite loads were different from those of infected BALB/c mice without α-lactose treatment. Although the expressions of Gal-9/receptor were significantly increased in B6 mice and Gal-1 and -3 were upregulated in both strains of mice upon ocular T. gondii infection, blockage of galectins did not change the ocular pathogenesis of genetic resistant BALB/c mice. However, IFN-α and -β were differently expressed in B6 and BALB/c mice, suggesting that type I IFNs may play a protective role in experimental OT. Copyright © Cambridge University Press 2017


Wang J.,Jinan University | Chen M.,Jinan University | Du J.,Jinan University
Medicine (United States) | Year: 2017

Rationale: Elbow injury in children by improper treatment or a delay of more than 3 weeks could lead to old unreduced Monteggia fracture, which are difficult to manage. Conservative or normal surgical methods usually fail. Patient concerns: Herein, we present a 6-year-old boy with sustaining injury approximately 1 month to his left elbow. Activity in his elbow was restricted, and his ability to extend his wrist and fingers was impaired. Diagnoses: Type III Monteggia elbow fracture-dislocation consisting of radial head dislocation and malunion of the ulna associated with posterior interosseous nerve palsy were confirmed, which requiring surgical treatment. Interventions: A closed reduction was performed with hyperplastic scar tissues erased and the radial head relocated. Outcomes: Follow-up 4 months later showed satisfactory recovery of function. Lessons: Forearm fractures in children may be misjudged, and that early anatomical reduction rather than conservative treatment may be required. © 2017 the Author(s). Published by Wolters Kluwer Health, Inc.


Wang C.,Jinan University | Xing L.,University of Massachusetts Dartmouth | Peng R.,University of Science and Technology Beijing | Pan Z.,Zhejiang Normal University
Reliability Engineering and System Safety | Year: 2017

A phased-mission system (PMS) involves multiple, consecutive, non-overlapping phases of operation. The system structure function and component failure behavior in a PMS can change from phase to phase, posing big challenges to the system reliability analysis. Further complicating the problem is the functional dependence (FDEP) behavior where the failure of certain component(s) causes other component(s) to become unusable or inaccessible or isolated. Previous studies have shown that FDEP can cause competitions between failure propagation and failure isolation in the time domain. While such competing failure effects have been well addressed in single-phase systems, only little work has focused on PMSs with a restrictive assumption that a single FDEP group exists in one phase of the mission. Many practical systems (e.g., computer systems and networks), however may involve multiple FDEP groups during the mission. Moreover, different FDEP groups can be dependent due to sharing some common components; they may appear in a single phase or multiple phases. This paper makes new contributions by modeling and analyzing reliability of PMSs subject to multiple FDEP groups through a Markov chain-based methodology. Propagated failures with both global and selective effects are considered. Four case studies are presented to demonstrate application of the proposed method. © 2017 Elsevier Ltd


Chen S.,Jinan University | Yan R.,Jinan University
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2016

Estimating unknown parameters for chaotic system is a key problem in the field of chaos control and synchronization. Through constructing an appropriate fitness function, parameter estimation of chaotic system could be converted to a multidimensional parameter optimization problem. In this paper, a new method base on improved boundary chicken swarm optimization (IBCSO) algorithm is proposed for solving the problem of parameter estimation in chaotic system. However, to the best of our knowledge, there is no published research work on chicken swarm optimization for parameters estimation of chaotic system. Computer simulation based on Lorenz system and comparisons with chicken swarm optimization, particle swarm optimization, and genetic algorithm shows the effectiveness and feasibility of the proposed method. © 2016 SPIE.


Wang W.,Jinan University | Zhang Y.,Jinan University
Pacific Asia Conference on Information Systems, PACIS 2015 - Proceedings | Year: 2015

Information technologies (IT) are increasingly prevalent in modern organizations. Given the important role of employee users in the IT implementation process, recent studies have acknowledged employee users' satisfaction with the implemented IT (IT satisfaction) as a salient aspect of job performance. This study extends prior literature by evaluating employees' job satisfaction as a surrogate of job performance induced by IT utilization. Drawing on IT performance literature, we develop a research model to examine the relationship between employee users' satisfaction with information and communication technologies (ICTs) and job satisfaction. To have a more comprehensive understanding about the relationship between IT satisfaction and job satisfaction, we introduce user-technology fit (job fit and professional fit), which concerns employee users' perceptions of IT use, as moderator. Moreover, we suggest that job fit and professional fit can mediate the positive impact of IT satisfaction on job satisfaction. Based on an online survey of 229 employee users of ICTs, this study finds that job fit positively moderates the impact of IT satisfaction on job satisfaction. Also, the results approve the mediating effect of both job fit and professional fit. This study contributes to IT performance literature by moving beyond researchers' focus on ITrelated outcomes, such as employee user satisfaction, to broader job-related outcomes.


Li C.-M.,Jinan University | Tang Z.-F.,Jinan University
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2016

Tungsten carbide thin films were prepared by dual-target magnetron sputtering deposition using pure tungsten target and carbon target. The influence of the substrate temperatures and the sputtering power of carbon target was studied. The results show that the suitable temperature of sputtering the tungsten carbide was 400℃. When the sputtering power of W target is 55 W, the XRD result indicates that the different thin films of W, W2C, WC1-x were obtained. Electrochemical investigations show that W2C, WC1-x both exhibited electro catalytic activity in the reaction of methanol oxidation and had a large real surface area. The real surface area of 1 cm2 W2C and WC1-x thin films were 33.32 cm2 and 64.68 cm2, respectively. © 2016, Chinese Ceramic Society. All right reserved.


Xu D.,Jinan University | Tan M.,Jinan University
Nonlinear Dynamics | Year: 2017

This paper focuses on the global asymptotic stability of complex-valued bidirectional associative memory (BAM) neutral-type neural networks with time delays. By virtue of homeomorphism theory, inequality techniques and Lyapunov functional, a set of delay-independent sufficient conditions is established for assuring the existence, uniqueness and global asymptotic stability of an equilibrium point of the considered complex-valued BAM neutral-type neural network model. The assumption on boundedness of the activation functions is not required, and the LMI-based criteria are easy to be checked and executed in practice. Finally, we give one example with simulation to show the applicability and effectiveness of our main results. © 2017 Springer Science+Business Media Dordrecht


Fan Z.,Jinan University
International Journal of Ad Hoc and Ubiquitous Computing | Year: 2017

Battery-powered sensor networks are often duty-cycled to conserve nodes energy and prolong network lifetime. In such intermittently-connected networks, the waiting time over multi-hop data forwarding dominates delivery latency, which is the main challenge and unacceptable for delay-sensitive applications. In this work, we address the optimisation problem for duty-cycled sensor network with transmission power control. In particular, we propose dynamic transmission power switch (DTPS), a cross-layer approach which jointly considers power control with multi-parent forwarding for the fast data collection. In DTPS, each node dynamically selects a sequence of transmission pairs to deliver data packets. Extensive simulation results show that our scheme can reduce both delivery delay and energy consumption for data collection applications. Copyright © 2017 Inderscience Enterprises Ltd.


Huang X.,Jinan University | Liu H.,Jinan University | Li R.,Jinan University
Gynecological Endocrinology | Year: 2017

Purpose: Integrin αvβ3 (ITG αvβ3) participates in the process of implantation between the embryo and the endometrium. This study investigated the effects of prostaglandin E2 (PGE2) on endometrial receptivity and implantation efficiency of the embryo, and their possible mechanisms. Methods: Quantitative real-time reverse transcription PCR (qRT-PCR) and western blotting were used to detect the changes in mRNA and protein levels of ITG αvβ3 in RL95-2 cells after administering PGE2. BeWo trophoblast cells and RL95-2 endometrial epithelial cells were used to establish an in vitro model, which was used to observe the adhesion rate and spreading efficiency between BeWo spheroids and RL95-2 cell monolayers after pretreatment with different concentrations of PGE2. Results: PGE2 at 200 nM increased the mRNA and protein levels of ITG αv significantly (p < 0.05); 100 nM PGE2 increased the mRNA and protein levels of ITG β3 significantly (p < 0.05). PGE2 at 200 nM increased significantly the adhesion and spreading efficiency of BeWo spheres to RL95-2 cell monolayers. Conclusions: An appropriate concentration of PGE2 might increase the expression of ITG αvβ3, which would, promote embryo adhesion and spreading efficiency. This study provides further evidence that increased expression of ITG αvβ3 might promote implantation by improving endometrial receptivity. © 2017 Informa UK Limited, trading as Taylor & Francis Group.


Shen S.Q.,Jinan University | Wang R.,Jinan University | Huang S.G.,Jinan University
Genetics and Molecular Research | Year: 2017

Stem cell factor (SCF), an important stem cell cytokine, has multiple functions. Fibroblasts (FBs), mature mast cells, endothelial cells (ECs), and eosinophil granulocytes can produce SCF in the inflammatory process. Therefore, we aimed to observe SCF expression in FBs, ECs, and macrophages (MPs) in periapical tissues in human chronic periapical disease and investigate the effects of cells expressing SCF in pathogenesis of the disease. Healthy (N = 20), periapical cyst (N = 15), and periapical granuloma (N = 15) tissues were fixed in 10% formalin for 48 h, embedded in paraffin, and stained with hematoxylin and eosin to observe histological changes. SCF expression was observed in FBs, ECs, and MPs in periapical tissues by double immunofluorescence. CD334, CD31, and CD14 are specific markers of FBs, ECs, and MPs, respectively. Results showed that densities of CD334-SCF double-positive FBs, CD31-SCF double-positive ECs, and CD14-SCF double-positive MPs were significantly increased in periapical tissue groups (P < 0.01). There were no significant differences in CD334-SCF double-positive FB and CD31-SCF double-positive EC levels between the two periapical tissue groups (P > 0.05). CD14- SCF double-positive MP density was considerably higher in periapical granulomas than in cysts (P < 0.01). FB, EC, and MP levels were significantly high and densities of CD334-SCF double-positive FBs, CD31-SCF double-positive ECs, and CD14-SCF double-positive MPs improved considerably in chronic periapical tissues, suggesting that the cells might be related to occurrence, development, and pathogenesis of chronic periapical disease. © 2017 The Authors.


Qiu S.,Jinan University | Liu J.,Jinan University | Xing F.,Jinan University
Cell Death and Differentiation | Year: 2017

Pyroptosis is a lytic form of cell death distinguished from apoptosis, ferroptosis, necrosis, necroptosis, NETosis, oncosis, pyronecrosis and autophagy. Proinflammatory caspases cleave a gasdermin D (GSDMD) protein to generate a 31 kDa N-terminal domain. The cleavage relieves the intramolecular inhibition on the gasdermin-N domain, which then moves to the plasma membrane to exhibit pore-forming activity. Thus, GSDMD acts as the final and direct executor of pyroptotic cell death. Owing to the selective targeting of the inner leaflet of the plasma membrane with the pore-forming that determines pyroptotic cell death, GSDMD could be a potential target to control cell death or extracellular bacterial infections. Intriguingly, other gasdermin family members also share similar N-terminal domains, but they present different cell death programs. Herein, we summarize features and functions of the novel player proteins in cell death, including GSDMD triggering pyroptosis, Gsdma3/GSDMA initiating autophagy/apoptosis and DFNA5 inducing apoptosis/secondary necrosis. The gasdermin N terminus appears to be a novel pore-forming protein. This provides novel insight into the underlying roles and mechanisms of lytic or nonlytic forms of programmed cell death, as well as their potential applications in inflammation-associated diseases. © 2017 The Author(s).


Zhu J.,Zhejiang University | Zhu J.,Jinan University | Yang H.,Zhejiang University
Journal of the Optical Society of America B: Optical Physics | Year: 2017

A mode solver based on the spectral element method with mesh adaptation is proposed to calculate the modal characteristics of a semivectorial field in open varying-index optical waveguides. General optical waveguides with varying refractive indices are studied for the transverse electric and transverse magnetic cases, where perfectly matched layers (PMLs) are used to truncate the unbounded waveguides. The optical field expanded by a suitable set of orthogonal basis points (Gauss-Lobatto-Legendre or Gauss-Chebyshev collocation points) through the spectral element method are all represented by processing of characterization of composite material through mesh adaptation. By this combined solver, a large number of accurate PML modes can be easily calculated. Our results are still found to be in good agreement with those produced by other numerical methods, but with more efficiency. Additionally, the relations of the PML modes' distribution to the parameters of PMLs are analyzed through the pitchfork phenomenon in the spectrum. © 2017 Optical Society of America.


Pan M.,Jinan University | Zhang J.,Jinan University
Genetics and Molecular Research | Year: 2017

Microarray gene expression technology provides a systematic approach to patient classification. However, microarray data pose a great computational challenge owing to their large dimensionality, small sample sizes, and potential correlations among genes. A recent study has shown that gene-gene correlations have a positive effect on the accuracy of classification models, in contrast to some previous results. In this study, a recently developed correlation-based classifier, the ensemble of random subspace (RS) Fisher linear discriminants (FLDs), was utilized. The impact of gene-gene correlations on the performance of this classifier and other classifiers was studied using simulated datasets and real datasets. A cross-validation framework was used to evaluate the performance of each classifier using the simulated datasets or real datasets, and misclassification rates (MRs) were computed. Using the simulated data, the average MRs of the correlation-based classifiers decreased as the correlations increased when there were more correlated genes. Using real data, the correlation-based classifiers outperformed the non-correlation-based classifiers, especially when the gene-gene correlations were high. The ensemble RS-FLD classifier is a potential state-of-the-art computational method. The correlation-based ensemble RS-FLD classifier was effective and benefited from gene-gene correlations, particularly when the correlations were high. © 2017 The Authors.


Liang Y.,City University of Hong Kong | Liang Y.,Jinan University | Jin L.,Jinan University | Guan B.-O.,Jinan University | Wang L.,City University of Hong Kong
Optics Letters | Year: 2017

Fast functional photoacoustic microscopy requires multiwavelength pulsed laser sources with high pulse repetition rates, short wavelength switching time, and sufficient pulse energies. Here, we report the development of a stimulatedRaman-scattering-based multi-wavelength pulsed laser source for fast functional photoacoustic imaging. The new laser source is pumped with a 532 nm 1 MHz pulsed laser. The 532 nm laser beam is split into two: one pumps a 5 m optical fiber to excite a 558 nm wavelength via stimulated Raman scattering; the other goes through a 50 m optical fiber to delay the 532 nm pulse by 220 ns. The two beams are combined and coupled into an optical fiber for photoacoustic excitation. As a result, the new laser source can generate 2 million pulses per second, switch wavelengths in 220 ns, and provide hundreds of nanojoules pulse energy for each wavelength. Using this laser source, we demonstrate optical-resolution photoacoustic imaging of microvascular structures and oxygen saturation in the mouse ear. The ultrashort wavelength switching time enables oxygen saturation imaging of flowing red blood cells, which is valuable for high-resolution functional imaging. © 2017 Optical Society of America.


Cai Y.,Jinan University
Proceedings - 2016 9th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics, CISP-BMEI 2016 | Year: 2016

Nucleosome which is the basic chromatin unit plays an important role in the genome packaging. We found the characteristics of nucleosome positioning among different genes of the human CD4 + cells. It was found that a linear combination of approximately 20 factors explaining the nucleosome positioning of each genome. In these linear combinations, a histone modification and the properties of the 6 DNA sequences are shared. Other factors are different. This study has a high resolution, which greatly simplifies the means to predict and understand the different genomic features in human CD4 + cells, providing a more accurate nucleosome occupancy. © 2016 IEEE.


Zhang F.-Z.,Jinan University | Chen X.-P.,Jinan University
Yantu Gongcheng Xuebao/Chinese Journal of Geotechnical Engineering | Year: 2010

A series of tests are performed to measure the variation of deformation and shear strength of unsaturated clays subjected to repeated drying and wetting cycles so as to study the effect of repeated drying and wetting cycles on unsaturated soil by using unsaturated triaxial apparatus. The test results show: firstly, the matric suction of the same soil moisture in SWCC reduces, and shrinkage behaviors of soil enhance during the pre-yield stage of shrinkage and do not change approximately during the post-yield stage of shrinkage; secondly, the compression index increases during the post-yield stage of isotropic compression, and the influence on compression behavior decreases with the increasing suction; and thirdly, a few samples subjected to repeated drying and wetting cycles exhibit a slide plane in triaxal shear tests. Consistently, the stress exhibits an obvious peak value and then decreases sharply with the increasing strain. In addition, repeated drying and wetting cycles not only decrease the triaxal shear coefficient φ' but also have an effect on coefficient φb with respect to matric suction to a certain extent. It indicates that the change of mechanical properties is irreversible after the unsaturated soil is subjected to repeated drying and wetting cycles.


Zhang W.,Fudan University | Zhang W.,Jinan University | Li N.,Fudan University | Bohinc K.,University of Ljubljana | And 2 more authors.
Physical Review Letters | Year: 2013

Using the techniques of optical microscopy and particle tracking, we measure the correlated diffusion in a monolayer of uniform silica spheres dispersed at a water-air interface. It is found that the correlated motion of the interfacial particles can be well described by two universal response functions, the normalized longitudinal and transverse diffusion coefficients DË(r/r0) and DË(r/r0), where r is the interparticle distance and r0=a(λS/ a)3/2 is a new scaling length, which depends on both the Saffman length λS and particle radius a. The obtained response functions characterize the crossover behavior of the colloidal monolayers from the subphase-dominated three-dimensional hydrodynamics at low surface coverage to the monolayer-dominated 2D hydrodynamics at high concentrations. The surface viscosity ηs(2) of the colloidal monolayer obtained by two-particle rheology compares well with the one-particle measurements. © 2013 American Physical Society.


Lan Y.,Jinan University | Qin J.,University of Science and Technology Beijing | Feng S.,Beijing Normal University
Physics Letters, Section A: General, Atomic and Solid State Physics | Year: 2013

The interplay between the superconducting gap and normal-state pseudogap in the bilayer cuprate superconductors is studied based on the kinetic energy driven superconducting mechanism. It is shown that the charge carrier interaction directly from the interlayer coherent hopping in the kinetic energy by exchanging spin excitations does not provide the contribution to the normal-state pseudogap in the particle-hole channel and superconducting gap in the particle-particle channel, while only the charge carrier interaction directly from the intralayer hopping in the kinetic energy by exchanging spin excitations induces the normal-state pseudogap in the particle-hole channel and superconducting gap in the particle-particle channel, and then the two-gap behavior is a universal feature for the single layer and bilayer cuprate superconductors. © 2013 Elsevier B.V. All rights reserved.


Wang S.,University of Macau | Qiu J.,Jinan University | Shi Z.,University of Macau | Shi Z.,Jinan University | And 2 more authors.
Biotechnology Advances | Year: 2015

Taxanes are one type of the most extensively used chemotherapeutic agents to treat cancers. However, their clinical use is severely limited by intrinsic and acquired resistance. A diverse variety of mechanisms has been implicated about taxane resistance, such as alterations of drug targets, overexpression of efflux transporters, defective apoptotic machineries, and barriers in drug transport. The deepening understanding of molecular mechanisms of taxane resistance has spawned a number of targets for reversing resistance. However, circumvention of taxane resistance would not only possess therapeutic potential, but also face with clinical challenge, which accelerates the development of optimal nanoscale delivery systems. This review highlights the current understanding on the mechanisms of taxane resistance, and provides a comprehensive analysis of various nanoscale delivery systems to reverse taxane resistance. © 2014 Elsevier Inc.


Zhang F.,Jinan University | Zhang F.,Guangdong Pharmaceutical University | Yang L.,Jinan University | Li Y.,Jinan University
Cancer Cell International | Year: 2012

Autoimmune phenomena were identified in many different cases of hematological diseases and solid tumors, which may be due to alterations in the expression and function of the NF-κB signaling pathway. Recently, a number of studies have shown that the deletion or mutation of A20, a negative regulator of NF-κB, is frequently found in lymphomas, suggesting that it may be a linker between the altered immune response and leukemogenesis. The aim of this review is to summarize current findings of the A20 biological functions and its molecular mechanism as a tumor suppressor and immune regulator. The identification of A20 mutations and deletions in lymphocytic malignancy and the predictive significance of these aberrations are also reviewed. © 2012 Zhang et al.; licensee BioMed Central Ltd.


Fu W.-H.,National Chi Nan University | Hsiao V.K.S.,National Chi Nan University | Tang J.-Y.,Jinan University | Wu M.-H.,National Chi Nan University | Chen Z.,Jinan University
Sensors and Actuators, B: Chemical | Year: 2011

This study presents a highly sensitive, all-fiber sensor for in situ detecting light. A fiber-optic light sensing platform was created by overlaying an in-line side-polished fiber (SPF) with a photoresponsive liquid crystal (P-LC) consisting of an azobenzene dye, a chiral dopant, and a nematic LC. The resulting P-LC overlaid SPF light sensor is sensitive to three different light sources, including 380 nm light emitting diode (LED), mercury lamp, and office ceiling lights. Under the light illumination, the energy of irradiation from short wavelengths of light (<450 nm) initiates the trans-to-cis photoisomerization of azobenzene. The photochemical LC-phase transition induced by the generated cis-moiety of azobenzene changes the refractive index of LC-overlaid side-polished area. Light illumination increased the attenuation of the input laser signal. After turning off the illumination, the attenuation returned to its original value, allowing the fiber-optic light sensor to be reused. The sensitivity of the resulting fiber-optic light sensor was 0.16 dB/(μW/cm2) with a detection limit of 5 μW/cm2 and 0.06 dB/lx with a detection limit of 45 lx when a 380 nm LED and office ceiling lights were used as illumination sources, respectively. The detection limit increased from 45 to 12 lx when P-LC containing 20 wt% azobenzene was used as light sensing material. The proposed fiber-based light sensor has potential use in harsh environments, such as severely humid and corrosive environments, which could damage mechanical and electronic light sensors. Crown Copyright © 2011 Published by Elsevier B.V. All rights reserved.


Liu J.,Hong Kong Polytechnic University | Guo N.,Hong Kong Polytechnic University | Li Z.,Jinan University | Yu C.,National University of Singapore | Lu C.,Hong Kong Polytechnic University
Optics Letters | Year: 2013

A microwave photonic filter with the highest reported quality factor (Q) value of 4895.31 is proposed and experimentally demonstrated by using two cascaded infinite impulse response (IIR) filters. Each IIR filter comprises both optical and electronic signals in a feedback loop and thus the loop length can be reduced without the need to consider the light coherence length. The Vernier effect enables a significant improvement of the free spectral ranges and Q values of the cascaded filter. The Q value of the proposed microwave photonic filter can be changed when the loop lengths of two cascaded filters are carefully adjusted. In addition, for a fixed Q, the frequency response of the filter can also be tuned by adjusting the bias of the Mach-Zehnder modulator in each loop. © 2013 Optical Society of America.


Li Z.,Sun Yat Sen University | Li Z.,Jinan University | Chen Z.,Jinan University | Hsiao V.K.S.,National Chi Nan University | And 3 more authors.
Optics Express | Year: 2012

This work presents an optically tunable chirped fiber Bragg grating (CFBG). The CFBG is obtained by a side-polished fiber Bragg grating (SPFBG) whose thickness of the residual cladding layer in the polished area (DRC) varies with position along the length of the grating, which is coated with a photoresponsive liquid crystal (LC) overlay. The reflection spectrum of the CFBG is tuned by refractive index (RI) modulation, which comes from the phase transition of the overlaid photoresponsive LC under ultraviolet (UV) light irradiation. The broadening in the reflection spectrum and corresponding shift in the central wavelength are observed with UV light irradiation density of 0.64mW/mm2. During the phase transition of the photoresponsive LC, the RI increase of the overlaid LC leads to the change of the CFBG reflection spectrum and the change is reversible and repeatable. The optically tunable CFBGs have potential use in optical DWDM system and an all-fiber telecommunication system. © 2012 Optical Society of America.


Yau S.-Y.,University of Hong Kong | Yau S.-Y.,University of Victoria | Li A.,University of Hong Kong | Li A.,Jinan University | And 2 more authors.
Neural Plasticity | Year: 2015

Adult hippocampal neurogenesis is a process involving the continuous generation of newborn neurons in the hippocampus of adult animals. Mounting evidence has suggested that hippocampal neurogenesis contributes to some forms of hippocampus-dependent learning and memory; however, the detailed mechanism concerning how this small number of newborn neurons could affect learning and memory remains unclear. In this review, we discuss the relationship between adult-born neurons and learning and memory, with a highlight on recently discovered potential roles of neurogenesis in pattern separation and forgetting. © 2015 Suk-yu Yau et al.


Zhao H.,Guangdong University of Technology | Jin H.,Jinan University | Cai J.,Jinan University
Materials Letters | Year: 2014

In this paper, nano-hydroxyapatite/chitosan (n-HA/CS) composites with high compressive strength were prepared by the urease-catalyzed method (UCM) under mild conditions. The samples were characterized by infrared spectroscopy (IR), X-ray diffraction (XRD), laser diffraction particle size analyzer (LDPSA) and transmission electron microscopy (TEM). The results showed that the shape, size and crystallinity index of nano-HA crystals in composites were similar to that of nature bone. The n-HA/CS samples prepared by UCM had smaller particle size compared with those prepared by traditional co-precipitation technique. Moreover, mechanical testing showed that the maximum compressive strength of the composite materials was 130.3±9.3 MPa, which was close to that of cortical bone. UCM has great potential in fabricating non-sintering and drug-loaded bioceramics with improved mechanical features in bone tissue engineering. © 2013 Elsevier B.V.


Tang H.,Sun Yat Sen University | Wu Z.,Fudan University | Zhang J.,Jinan University | Su B.,Biomedical Research Institute | And 2 more authors.
Molecular Medicine Reports | Year: 2013

Oral squamous cell carcinoma (OSCC) is a common and lethal malignancy. Thus, improvement in current knowledge of molecular changes associated with OSCC is urgently needed to explore novel avenues of diagnostics and treatment of this disease. While aberrant expression of long non.coding RNAs (lncRNAs) has been functionally associated with certain types of cancer, including lung, breast and prostate carcinomas, their expression pattern and biological relevance in OSCC is currently unknown. In the present study, the relative abundance of a collection of lncRNAs in tissue or saliva samples from OSCC patients was investigated. It was shown that subsets of lncRNAs are expressed across non.tumor, tumor and metastatic tissue samples. Some detected lncRNAs were shown to be aberrantly expressed in cases of oral cancer and metastasis. Moreover, whole saliva contained a detectable amount of some lncRNAs, which appeared to be potential markers for OSCC. These findings suggest that the detection of lncRNAs in saliva may be used as a noninvasive and rapid diagnostic tool for the diagnosis of oral cancer.


Han X.-H.,Guangdong University of Technology | Xue S.-J.,Jinan University
Jisuanji Jicheng Zhizao Xitong/Computer Integrated Manufacturing Systems, CIMS | Year: 2010

In order to increase operational efficiency, the reverse channel decision was studied based on evolutionary game in the closed-loop supply chain with competitive manufactures and one dominant retailer. Results indicated that the competition among manufacturers, the collection difficulty and remanufacturing cost saving influenced the evolution result of reverse channel together. And by adopting subsidy policy, government encouraged manufacturers to choose direct reverse channel, which was more benefit to customers and society.


Niu B.,Shenzhen University | Niu B.,Chinese Academy of science | Niu B.,China Institute of Technology | Wang H.,Shenzhen University | And 2 more authors.
Neurocomputing | Year: 2013

This paper describes a novel Bacterial Foraging Optimization (BFO) approach to multi-objective optimization, called Multi-objective Bacterial Foraging Optimization (MBFO). The objectives in the Multi-objective Bacterial Foraging Optimization are maintained by a fitness survive mechanism. Bacteria with the smaller health values have the better chance to survive. Meanwhile, the main goal of multi-objective optimization problems is to obtain a superior non-dominated front which is closed to the true Pareto front. With identification of such features, the idea of integration between health sorting approach and pareto dominance mechanism are developed to search for Pareto-optimal set of problems. Moreover, strategy keeping a certain unfeasible border solutions based on a given probability is considered to improve the diversity of individuals. In addition, two different performance metrics: Diversity and Generational Distance are introduced as well to evaluate multi-objective optimization problems. Compared to two other multi-objective optimization evolutionary algorithms MOPSO and NSGA-II, simulation results show that in most cases, the proposed MBFO is able to find a much better spread of solutions and convergence to the true Pareto-optimal front faster. It suggests that MBFO is very promising in dealing with ordinary multi-objective optimization problems. © 2012 Elsevier B.V.


Tang S.,Jinan University | Meng X.,Central Hospital of Yidu
Turkish Neurosurgery | Year: 2011

Aim: The restoration of disc space height of fused segment is essential in anterior lumbar interbody fusion, while the disc space height in many cases decreased postoperatively, which may adversely aggravate the adjacent segmental degeneration. However, no literature available focused on the issue. Material and Methods: A normal healthy finite element model of L3-5 and four anterior lumbar interbody fusion models with different disc space height of fused segment were developed. 800 N compressive loading plus 10 Nm moments simulating exion, extension, lateral bending and axial rotation were imposed on L3 superior endplate. The intradiscal pressure, the intersegmental rotation, the tresca stress and contact force of facet joints in L3-4 were investigated. Results: Anterior lumbar interbody fusion with severely decreased disc space height presented with the highest values of the four parameters, and the normal healthy model presented with the lowest values except, under extension, the contact force of facet joints in normal healthy model is higher than that in normal anterior lumbar interbody fusion model. With disc space height decrease, the values of parameters in each anterior lumbar interbody fusion model increase gradually. Conclusion: Anterior lumbar interbody fusion with decreased disc space height aggravate the adjacent segmental degeneration more adversely.


Man Z.,Swinburne University of Technology | Wang W.,Defence Science and Technology Organisation, Australia | Khoo S.,Deakin University | Yin J.,Jinan University
Mechanical Systems and Signal Processing | Year: 2012

In this paper, the synchronous signal average of gear mesh vibration signals is modelled with the multiple modulated sinusoidal representations. The signal model parameters are optimised against the measured signal averages by using the batch learning of the least squares technique. With the optimal signal model, all components of a gear mesh vibration signal, including the amplitude modulations, the phase modulations and the impulse vibration component induced by gear tooth cracking, are identified and analysed with insight of the gear tooth crack development and propagation. In particular, the energy distribution of the impulse vibration signal, extracted from the optimal signal model, provides sufficient information for monitoring and diagnosing the evolution of the tooth cracking process, leading to the prognosis of gear tooth cracking. The new methodologies for gear mesh signal modelling and the diagnosis of the gear tooth fault development and propagation are validated with a set of rig test data, which has shown excellent performance. © 2012 Elsevier Ltd.


Yan C.,Guangdong Pharmaceutical University | Yin Y.,Jinan University | Zhang D.,Guangdong Medical College | Yang W.,Jinan University | Yu R.,Jinan University
Carbohydrate Polymers | Year: 2013

The shrub, Taxus yunnanensis is famed as the source of the important anticancer drug, paclitaxel. But research on its polysaccharides contents has been scarce. The present research aimed to investigate the polysaccharide content of T. yunnanensis leaves and study the antitumor activities of isolated polysaccharide( s) using human tumor cells (K-562 and MCF). A novel heteropolysaccharide (TMP70W) was isolated and purified by anion-exchange and gel-permeation chromatography. Its molecular weight was 36.94 kDa and structural features were elucidated by partial acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, GC-MS, HPAEC-PAD, FT-IR, and NMR. The repeating unit of TMP70W had a backbone composed of (1→5)-linked-α-l- Araf, (1→2,5)-linked-α-l-Araf, and (1→6)-linked-α-d-Galp with a branch of α-d-Glcp-(1→2)-α-d-Galp-(1→ at the position of C-2 of arabinose. TMP70W displayed mild cytotoxicity against K562 cells with the IC50 value of 39.63 ± 2.37 μg/mL and inhibitory activity against MCF-7 cells (32.08 ± 0.39% at the concentration of 400 μg/mL) in a concentration-dependent manner. © 2013 Elsevier Ltd. All rights reserved.


Hu S.,Beijing Normal University | Jin H.,South China Normal University | Chen Z.,Jinan University | Mo L.,South China Normal University | Liu J.,Beijing Normal University
Cortex | Year: 2013

Previous functional magnetic resonance imaging (fMRI) studies have identified several regions in the ventral visual pathway that are specialized for processing faces, words and general objects. However, little is known about the origin of the functional selectivity of these regions. Here, we reported a pediatric patient who suffered a left occipitoparietal lesion in the first year after birth from a subdural hematoma. After the hematoma was removed at the age of six, the hemianopia in the right visual field was alleviated, and no obvious deficits in low-level vision were observed in the patient at the age of twelve. In line with the behavioral observations, meridian mappings with fMRI showed that the early visual cortex of the left hemisphere was significantly activated, which was similar to that of the intact right hemisphere. However, the left ventral temporal cortex failed to show selective responses for faces, words and objects, which were in contrast to the normal selective responses for these objects in the right counterpart. Therefore, it is likely that the development of object selectivity in the ventral temporal cortex depends on visual inputs from the early visual cortex at an early age. © 2013 Elsevier Ltd.


Cui Y.-H.,Guangzhou University | Pan H.-W.,Jinan University | Pan H.-W.,First Affiliated Hospital
American Journal of Clinical Nutrition | Year: 2013

Background: Observational studies have been inconsistent regarding the association between blood antioxidants or vitamins and risk of age-related cataract. Objective: We performed a meta-analysis to determine whether an association exists between blood levels of antioxidants or vitamins and age-related cataract in observational studies. Design: We searched PubMed, EMBASE, and the Web of Science for relevant studies from inception to October 2012. Study-specific risk estimates were combined by using a random-effects model. Results: A total of 13 studies with 18,999 participants were involved in this meta-analysis. A pooled estimate showed vitamin E (OR: 0.75; 95% CI: 0.58, 0.96), α-carotene (OR: 0.72; 95% CI: 0.59, 0.88), lutein (OR: 0.75; 95% CI: 0.65, 0.87), and zeaxanthin (OR: 0.70; 95% CI: 0.60, 0.82) were inversely associated with age-related cataract. Vitamins A (OR: 0.69; 95% CI: 0.58, 0.83) and C (OR: 0.67; 95% CI: 0.57, 0.78) were inversely associated with age-related cataract in Asian populations but not in Western populations. β-Carotene (OR: 0.90; 95% CI: 0.78, 1.05), lycopene (OR: 0.86; 95% CI: 0.65, 1.15), and β-cryptoxanthin (OR: 0.83; 95% CI: 0.68, 1.02) had no significant association with risk of cataract. Conclusions: This meta-analysis provides additional evidence supporting the view that blood levels of certain antioxidants are inversely associated with risk of age-related cataract. However, the role of antioxidant or vitamin supplement intake in preventing cataract should be further investigated in interventional studies. © 2013 American Society for Nutrition.


Wen Y.-D.,National University of Singapore | Wang H.,National University of Singapore | Kho S.-H.,National University of Singapore | Rinkiko S.,Jinan University | And 3 more authors.
PLoS ONE | Year: 2013

Background: Hydrogen sulfide (H2S) has been shown to have cytoprotective effects in models of hypertension, ischemia/reperfusion and Alzheimer's disease. However, little is known about its effects or mechanisms of action in atherosclerosis. Therefore, in the current study we evaluated the pharmacological effects of H2S on antioxidant defenses and mitochondria protection against hydrogen peroxide (H2O2) induced endothelial cells damage. Methodology and Principal Findings: H2S, at non-cytotoxic levels, exerts a concentration dependent protective effect in human umbilical vein endothelial cells (HUVECs) exposed to H2O2. Analysis of ATP synthesis, mitochondrial membrane potential (ΔΨm) and cytochrome c release from mitochondria indicated that mitochondrial function was preserved by pretreatment with H2S. In contrast, in H2O2 exposed endothelial cells mitochondria appeared swollen or ruptured. In additional experiments, H2S was also found to preserve the activities and protein expressions levels of the antioxidants enzymes, superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase in H2O2 exposed cells. ROS and lipid peroxidation, as assessed by measuring H2DCFDA, dihydroethidium (DHE), diphenyl-l-pyrenylphosphine (DPPP) and malonaldehyde (MDA) levels, were also inhibited by H2S treatment. Interestingly, in the current model, D, L-propargylglycine (PAG), a selective inhibitor of cystathionine γ-lyase (CSE), abolished the protective effects of H2S donors. Innovation: This study is the first to show that H2S can inhibit H2O2 mediated mitochondrial dysfunction in human endothelial cells by preserving antioxidant defences. Significance: H2S may protect against atherosclerosis by preventing H2O2 induced injury to endothelial cells. These effects appear to be mediated via the preservation of mitochondrial function and by reducing the deleterious effects of oxidative stress. © 2013 Wen et al.


Tan C.,Sun Yat Sen University | Hu S.,Guangdong University of Technology | Liu J.,Sun Yat Sen University | Liu J.,Jinan University | Ji L.,Sun Yat Sen University
European Journal of Medicinal Chemistry | Year: 2011

Two new ruthenium complexes, trans,cis,cis-[RuCl2(DMSO) 2(H2biim)] (1) and mer-[RuCl3(DMSO)(H 2biim)] (2) (DMSO = dimethyl sulfoxide and H2biim = 2,2′-biimidazole), have been synthesized and fully characterized by single-crystal X-ray analysis. The less stable complex 2 is more cytotoxic against the four human cancer cell lines tested than 1. Further studies show that 1 and 2 exhibit cell growth inhibition by triggering G0/G1 cell cycle arrest and mitochondria-mediated apoptosis. Additionally, complex 2 exerts potent inhibitory effects on the adhesion and migration of human cancer cells comparable to that of NAMI-A ([ImH][trans-[RuCl4(Im)(DMSO-S)], Im = imidazole). Target validation studies show that cyclin-dependent kinases (CDKs), other than DNA, are more likely to be targets of 1 and 2. © 2011 Elsevier Masson SAS. All rights reserved.


Chen D.,China Institute of Technology | Yao D.,China Institute of Technology | Xie C.,China Institute of Technology | Xie C.,Jinan University | And 2 more authors.
Food Control | Year: 2014

Developing the rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper, we developed a label-free aptasensor for electrochemical detection of tetracycline. The reorganization of tetracycline binding aptamer was confirmed by Isothermal Titration Calorimetry, Kd=5.18×10-5molL-1. According to the electrochemical impendence spectroscopy (EIS) analysis, there was a linear relationship between the log concentration of tetracycline and the charge transfer resistance (δRet) from 5.0 to 5.0×103ngmL-1 of the tetracycline conc. The detection limit was 1.0ngmL-1 within a detection time of 15min. The average of assemble rate Q was at 82.4% with a differential batches' RSD of 4.6%. The current change of this aptasensor lies within at 8.5% after a storage of 15 days under 4°C. The result aptasensor had shown a good reproducibility with an acceptable stability in tetracycline detection. The recoveries of TET in spiked milk samples were in the range of 90.0-95.7%. © 2014 Elsevier Ltd.


Zhang W.,South China Normal University | Zhang W.,China Institute of Technology | Wang X.,Jinan University | Chen T.,South China Normal University
Cellular Signalling | Year: 2012

Our recent study have shown that resveratrol (RV), a natural plant polyphenol found in red grape skins as well as other food product, induced apoptosis via the downstream factors, caspase-independent AIF and to lesser extent caspase-9, of intrinsic apoptosis pathway in human lung adenocarcinoma (ASTC-a-1) cells. This report is designed to explore the roles of the upstream mediators of the intrinsic pathway, such as Bak/Bax, Bim, Puma and Noxa, during RV-induced apoptosis in human lung adenocarcinoma (ASTC-a-1 and A549) cell lines. RV treatment remarkably induced the activation of Bak but not Bax, and silencing Bak but not Bax by shRNA almost completely prevented RV-induced cell death, mitochondrial dysfunction and also largely prevented RV-induced AIF release, demonstrating the preferential engagement of Bak but not Bax during RV-induced apoptosis. In addition, although RV treatment induced a significant degradation of Mcl-1, knockdown of Mcl-1 by shRNA only modestly increased RV-induced Bak activation. Interestingly, silencing Bim but not Puma and Noxa remarkably attenuated RV-induced cell death, loss of mitochondrial membrane potential, and Bak activation, suggesting the important roles of Bim. Collectively, our findings for the first time demonstrate that RV induces apoptosis dominantly via a Bak- but not Bax-mediated AIF-dependent mitochondrial apoptotic signaling pathway in which Bim but not Puma and Noxa may supply the force to trigger Bak activation and subsequent apoptosis in both ASTC-a-1 and A549 cell lines. © 2012 Elsevier Inc..


Chen Z.,Jinan University | Hsiao V.K.S.,Jinan University | Hsiao V.K.S.,National Chi Nan University | Li X.,Jinan University | And 3 more authors.
Optics Express | Year: 2011

This paper demonstrates light-induced tuning of the optical spectrum by a microfiber-knot resonator overlaid with a photoresponsive liquid crystal (LC) mixture containing photosensitive diluents (non-mesogenic azobenzene molecules), a chiral dopant and a nematic LC. The high-quality resonator is made by drawing a single mode fiber to a micro-size diameter and causing the microfiber to self-twist into a knot. A thin layer of a photosensitive mixture was placed on the overlap (knot) area and gentle heating was used to obtain a uniform thin film which coated the fiber's surface. Upon irradiation with UV light, noticeable changes to the peak resonance wavelengths were observed which we associate with a local change in the refractive index (RI) in the fiber's tapering area. Repeatable and reversible spectral shifting (0.15 nm) of the resonance wavelength is demonstrated by irradiation with 50 mW/cm2 UV light. © 2011 Optical Society of America.


Liu G.-Y.,Guangdong University of Technology | Hu J.-M.,Wuhan University | Wang H.-L.,Jinan University
Scientometrics | Year: 2012

The aim of this study is to map the intellectual structure of digital library (DL) field in China during the period of 2002-2011. Co-word analysis was employed to reveal the patterns of DL field in China through measuring the association strength of keywords in relevant journals. Data was collected from Chinese Journal Full-Text Database during the period of 2002-2011. And then, the co-occurrence matrix of keywords was analyzed by the methods of multivariate statistical analysis and social network analysis. The results mainly include five parts: seven clusters of keywords, a two-dimensional map, the density and centrality of clusters, a strategic diagram, and a relation network. The results show that there are some hot research topics and marginal topics in DL field in China, but the research topics are relatively decentralized compared with the international studies. © 2011 Akadémiai Kiadó, Budapest, Hungary.


Yaua S.Y.,University of Hong Kong | Yaua S.Y.,University of Victoria | Lia A.,University of Hong Kong | Hooc R.L.C.,University of Hong Kong | And 6 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2014

Adiponectin (ADN) is an adipocyte-secreted protein with insulinsensitizing, antidiabetic, antiinflammatory, and antiatherogenic properties. Evidence is also accumulating that ADN has neuroprotective activities, yet the underlying mechanism remains elusive. Here we show that ADN could pass through the blood-brain barrier, and elevating its levels in the brain increased cell proliferation and decreased depression-like behaviors. ADN deficiency did not reduce the basal hippocampal neurogenesis or neuronal differentiation but diminished the effectiveness of exercise in increasing hippocampal neurogenesis. Furthermore, exerciseinduced reduction in depression-like behaviors was abrogated in ADN-deficient mice, and this impairment in ADN-deficient mice was accompanied by defective running-induced phosphorylation of AMP-activated protein kinase (AMPK) in the hippocampal tissue. In vitro analyses indicated that ADN itself could increase cell proliferation of both hippocampal progenitor cells and Neuro2a neuroblastoma cells. The neurogenic effects of ADN were mediated by the ADN receptor 1 (ADNR1), because siRNA targeting ADNR1, but not ADNR2, inhibited the capacity of ADN to enhance cell proliferation. These data suggest that adiponectin may play a significant role in mediating the effects of exercise on hippocampal neurogenesis and depression, possibly by activation of the ADNR1/AMPK signaling pathways, and also raise the possibility that adiponectin and its agonists may represent a promising therapeutic treatment for depression.


Feng B.H.,Guangdong Pharmaceutical University | Zhang Z.Y.,Jinan University
Advanced Materials Research | Year: 2011

A new chitosan derivate (CMC-g-RA) was prepared by grafting ricinoleic acid anhydride (RA) on carboxymethy chitosan (CMC) molecules. The reaction factors such as molecular weight, mole ratio of anhydride to amino, temperature and solvent were examined. Nanoparticles were obtained by blending botanical insecticide capsaicin (Cap) and CMC-g-RA. Surface shape, particle size, polydispersity index, surface charge, LE and stability of Cap/CMC-g-RA nanoparticles were characterized. The results showed that lower molecular weight was of advantage to higher degree of RA substitution. CMC-g-RA and Cap formed drug delivery nanoparticles with spherical shape and negative surface charge in water. The increased concentration of drug or carrier resulted in higher zeta potential and narrower polydispersity index (PDI) of Cap/CMC-g-RA. The loading efficiency (LE) of nanoparticles was up to 0.86, which provided efficiently protection for Cap, extended drug releasing period and dosage. © (2011) Trans Tech Publications.


Yin J.,Jinan University | Wang W.,Defence Science and Technology Organisation, Australia | Man Z.,Swinburne University of Technology | Khoo S.,Deakin University
Mechanical Systems and Signal Processing | Year: 2013

The purpose of this paper is to reveal the pattern of gear tooth crack growth under variable-amplitude loading. To this end, a nonlinear dynamic model is proposed to describe the gear tooth crack growth. The state variables of the model are crack length and crack opening stress. The dynamics of crack growth is modeled as a modified Paris equation based on the concept of crack closure. A nonlinear second-order autoregressive equation is developed to model the dynamic behavior of the crack opening stresses. The model parameters are estimated by means of a two-step estimation method because of relatively small sample size of crack length data for G6 gear tests. The model is also validated with the crack growth data of the G6 gear. © 2013 Elsevier Ltd.


Wang S.,University of Macau | Chen R.,University of Macau | Zhong Z.,University of Macau | Shi Z.,Jinan University | And 2 more authors.
American Journal of Chinese Medicine | Year: 2014

Drug resistance remains an on-going challenge in breast cancer chemotherapy. Combination of two or more drugs is an effective strategy to access context-specific multiple targets and overcome undesirable toxicity that is almost inevitable in single-drug chemotherapy. Many plant food-derived polyphenolic compounds have been proven to modulate many key factors responsible for cancer drug resistance, which makes them a promising group of low toxicity candidates for reversing cancer resistance. In this study, we analyzed the combination effect of two chemopreventive polyphenols, curcumin (Cur) and epigallocatechin-3-gallate (EGCG), in combating resistant breast cancer. Our present results showed that EGCG significantly enhanced the growth inhibition and apoptosis in both doxorubicin (DOX)-sensitive and resistant MCF-7 cells induced by Cur. The mechanism may be related to the further activation of caspase-dependent apoptotic signaling pathways and the enhanced cellular incorporation of Cur by inhibiting P-glycoprotein (P-gp) pump function. Moreover, Cur and EGCG in combination could enhance the toxicity of DOX and increase the intracellular level of DOX in resistant MCF-7 cells. Our findings with this practical combination of Cur and EGCG encourage us to move on to a promising strategy for successful treatment of human breast cancer resistance by combining two low-toxic chemotherapeutic agents from diet. © 2014 World Scientific Publishing Company and Institute for Advanced Research in Asian Science and Medicine.


Yin J.,Jinan University | Wang W.,Defence Science and Technology Organisation, Australia | Man Z.,Swinburne University of Technology | Khoo S.,Deakin University
Information Sciences | Year: 2014

A statistical model of gear mesh vibration signals is proposed in this paper. It is seen that the sample mean is first removed from the gear mesh vibration signal, and the remaining signal components are approximated with a set of sinusoidal functions in the signal model. The signal model parameters can be estimated by the least-square method and the optimal model order is determined based on the Akaike Information Criterion (AIC) or the modified Bayesian Information Criterion (BIC). Within the framework of the signal model, to perform the gear fault diagnosis and analysis, the residual signal between the synchronous signal average and the output of the optimal signal model is further computed and the corresponding kurtosis values are employed for efficiently detecting the gear tooth fault. © 2013 Elsevier Inc. All rights reserved.


Yin J.,Jinan University | Khoo S.,Deakin University | Man Z.,Swinburne University of Technology | Yu X.,RMIT University
Automatica | Year: 2011

This paper presents a new definition of finite-time stability for stochastic nonlinear systems. This definition involves stability in probability and finite-time attractiveness in probability. An important Lyapunov theorem on finite-time stability for stochastic nonlinear systems is established. A theorem extending the stochastic Lyapunov theorem is also proved. Moreover, an example and a lemma are presented to illustrate the scope of extension. A useful inequality, extended from Bihari's inequality, is derived, which plays an important role in showing the Lyapunov theorem. Finally, a Lyapunov theorem on finite-time instability is proved, which states that almost surely globally asymptotical stability is not equivalent to finite-time stability for some stochastic systems. Two simulation examples are given to illustrate the theoretical analysis. © 2011 Elsevier Ltd. All rights reserved.


Chen X.-P.,Jinan University
Yantu Lixue/Rock and Soil Mechanics | Year: 2013

As the engineering practice indicated that the rheological behavior is obviously under unloading of the soft clay. According to the typical pit unloading paths, a series of unloading rheological tests were done with stress-control triaxial devices under different unloading conditions. The unloading conditions include confining stress, unloading stress paths and drainage conditions. Through experiments, the unloading rheological deformation of soft clay can be divided into instantaneous rebound deformation and hysteretic rebound deformation. When confining pressure and axial pressure unloaded under the same time, the initial strain rate is relatively larger on a short time after unloading; at the same condition, the hysteretic rebound effect is not obvious because the soil have negative pore pressure. When the axial pressure unloaded, the hysteretic rebound effect is more obvious. At the same unloading path, the rheological properties are more obvious under drainage condition because of that the soil makes water swelling, and the negative pore pressure will eventually be dissipated. Based on the test results, the linear function between the Merchant rheological model parameters and unloading conditions is established; and then established the soft soil unloading rheological empirical model which can consider the effects of the confining pressure and the unloading path. These conclusions are very useful to research the soft clay rheological deformation model and the calculation of excavation unloading rheological deformation.


Khoo S.,Deakin University | Khoo S.,Jinan University | Khoo S.,Swinburne University of Technology | Yin J.,Jinan University | And 2 more authors.
Automatica | Year: 2013

In this paper, we investigate the problem of almost surely finite-time stabilization of a class of stochastic nonlinear systems. Based on the recently proposed almost surely finite-time stability theorem in Yin, Khoo, Man, and Yu (2011), we prove that, almost surely global finite-time stability of stochastic nonlinear systems in strict-feedback form can be guaranteed by a continuous control law. A systematic design algorithm is developed for the construction of the controller. Simulation results are given to illustrate the theoretical analysis. Crown Copyright © 2013 Published by Elsevier Ltd. All rights reserved.


Dong D.,Jinan University | Wang H.,Jinan University | Wang Y.,Jinan University
Molecular Pharmaceutics | Year: 2015

Efflux of glucuronide is facilitated by the membrane transporters including BCRP and MRPs. In this study, we aimed to determine the effects of transporter expression on glucuronide efflux and cellular glucuronidation. Single efflux transporter (i.e., BCRP, MRP1, MRP3, or MRP4) was stably knocked-down in UGT1A1-overexpressing HeLa cells. Knock-down of transporters was performed by stable transfection of short-hairpin RNA (shRNA) using lentiviral vectors. Glucuronidation and glucuronide transport in the cells were characterized using three different aglycones (i.e., genistein, apigenin, and emodin) with distinct metabolic activities. BCRP knock-down resulted in significant reductions in excretion of glucuronides (42.9% for genistein glucuronide (GG), 21.1% for apigenin glucuronide (AG), and 33.7% for emodin glucuronide (EG); p < 0.01) and in cellular glucuronidation (38.3% for genistein, 38.6% for apigenin, and 34.7% for emodin; p < 0.01). Knock-down of a MRP transporter led to substantial decreases in excretion of GG (32.3% for MRP1, 36.7% for MRP3, and 36.6% for MRP4; p < 0.01) and AG (59.3% for MRP1, 24.7% for MRP3, and 34.1% for MRP4; p < 0.01). Also, cellular glucuronidation of genistein (38.3% for MRP1, 32.3% for MRP3, and 31.1% for MRP4; p < 0.01) and apigenin (40.6% for MRP1, 32.4% for MRP3, and 34.6% for MRP4; p < 0.001) was markedly suppressed. By contrast, silencing of MRPs did not cause any changes in either excretion of EG or cellular glucuronidation of emodin. In conclusion, cellular glucuronidation was significantly altered by decreasing expression of efflux transporters, revealing a strong interplay of glucuronidation with efflux transport. © 2015 American Chemical Society.


Li H.,University of Macau | Gao L.,Jinan University
Acta Mathematica Scientia | Year: 2015

Applying Nevanlinna theory of the value distribution of meromorphic functions, we mainly study the growth and some other properties of meromorphic solutions of the type of system of complex differential and difference equations of the following form (*){∑j=1nαj(z)f1(λj1)(z+cj)=R2(z,f2(z)),∑j=1nβj(z)f2λj2(z+cj)=R1(z,f1(z)). where λij (j = 1,2,···, n; i = 1,2) are finite non-negative integers, and cj (j = 1,2,·,n) are distinct, nonzero complex numbers, αj(z), βj(z) (j = 1,2,·,n) are small functions relative to fi(z) (i = 1,2) respectively, Ri(z,f(z)) (i = 1,2) are rational in fi(z) (i = 1,2) with coefficients which are small functions of fi(z)(i = 1,2) respectively. © 2015 Wuhan Institute of Physics and Mathematics.


Wang Y.,University of Macau | He Q.-Y.,Jinan University | Chiu J.-F.,Shantou University | Chiu J.-F.,University of Hong Kong
European Journal of Pharmacology | Year: 2014

Saponins have shown promise in cancer prevention and therapy; however, little is known about the detailed signaling pathways underlying their anticancer activities. In the present study, we examined the mechanisms of action of dioscin, a glucosides saponin isolated from Polygonatum zanlanscianense pump, in human myeloblast leukemia HL-60 cells. Dioscin suppressed HL-60 cell growth in a dose-dependent manner. This inhibition was due to the induction of apoptosis as revealed by the externalization of phosphatidylserine, and cleavages of lamin A/C and PARP-1. Treatment with dioscin induced apoptosis through activation of caspases 3, 7, 8, 9, and 10. Phosphorylation of p38 MAPK and JNK contributed to dioscin-induced apoptosis upstream of caspase activation. Using various inhibitors and antioxidant agents, we found that mitochondrial derived reactive oxygen species and depletion of mitochondrial transmembrane potential lead to the phosphorylation of p38 MAPK and JNK. Taken together, our results demonstrated that dioscin induces apoptosis by activation of p38 MAPK and JNK through the caspase-dependent mitochondrial death pathway. This work suggests that dioscin may be used as a drug lead for the treatment of myeloblast leukemia. © 2014 Elsevier B.V.


Lee T.M.C.,University of Hong Kong | Wong M.L.,University of Hong Kong | Lau B.W.M.,Hong Kong Polytechnic University | Lee J.C.D.,University of Hong Kong | And 3 more authors.
Psychoneuroendocrinology | Year: 2014

Recent findings have suggested that aerobic exercise may have a positive effect on brain functioning, in addition to its well-recognized beneficial effects on human physiology. This study confirmed the cognitive effects of aerobic exercise on the human brain. It also examined the relationships between exercise and the serum levels of neurotrophic factors (BDNF, IGI-1, and VEGF). A total of 91 healthy teens who exercised regularly participated in this study. A between-group design was adopted to compare cognitive functioning subserved by the frontal and temporal brain regions and the serum levels of neurotrophic factors between 45 regular exercisers and 46 matched controls. The exercisers performed significantly better than the controls on the frontal and temporal functioning parameters measured. This beneficial cognitive effect was region-specific because no such positive cognitive effect on task-tapping occipital functioning was observed. With respect to the serum levels of the neurotrophic factors, a negative correlation between neurotrophic factors (BDNF and VEGF) with frontal and medial-temporal lobe function was revealed. Furthermore, the levels of BDNF and VEGF interacted with exercise status in predicting frontal and temporal lobe function. This is the first report of the interaction effects of exercise and neurotrophic factors on cognitive functioning. Herein, we report preliminary evidence of the beneficial effects of regular aerobic exercise in improving cognitive functions in teens. These beneficial effects are region-specific and are associated with the serum levels of neurotrophic factors. Our findings lay the path for future studies looking at ways to translate these beneficial effects to therapeutic strategies for adolescents. © 2013 The Authors.


Li J.,Jinan University | Yu C.,National University of Singapore | Yu C.,Institute for Infocomm Research | Li Z.,Jinan University
Optics Letters | Year: 2014

A novel polarization-modulator-based complementary frequency shifter (PCFS) has been proposed and then used to implement the generation of a frequency-locked multicarrier with single- and dual-recirculating frequency shifting loops, respectively. The transfer functions and output properties of PCFS and PCFS-based multicarrier generator have been studied theoretically. Based on our simulation results through VPItransmissionMaker software, 100 stable carriers have been obtained with acceptable flatness while no DC bias control is required. The results show that the proposed PCFS has the potential to become a commercial product and then used in various scenarios. © 2014 Optical Society of America.


Xie Q.,Armed Police Corps Hospital | Xie Q.,Jinan University | Yan Y.,Jinan University | Huang Z.,Jinan University | And 2 more authors.
Neuropathology | Year: 2014

MicroRNAs (miRNAs) are short regulatory RNAs that negatively regulate protein biosynthesis at the post-transcriptional level and participate in the pathogenesis of different types of human cancers, including glioblastoma. In particular, the levels of miRNA-221 are overexpressed in many cancers and miRNA-221 exerts its functions as an oncogene. Nevertheless, the roles of miRNA-221 in carmustine (BCNU)-resistant glioma cells have not been totally elucidated. In the present study, we explored the effects of miRNA-221 on BCNU-resistant glioma cells and the possible molecular mechanisms by which miRNA-221 mediated the cell proliferation, survival, apoptosis and BCNU resistance were investigated. We found that miR-221 was overexpressed in glioma cells, including BCNU-resistant cells. Moreover, we found that miR-221 regulated cell proliferation and BCNU resistance in glioma cells. Overexpression of miR-221 led to cell survival and BCNU resistance and reduced cell apoptosis induced by BCNU, whereas knockdown of miR-221 inhibited cell proliferation and prompted BCNU sensitivity and cell apoptosis. Further investigation revealed that miR-221 down-regulated PTEN and activated Akt, which resulted in cell survival and BCNU resistance. Overexpression of PTEN lacking 3'UTR or PI3-K/Akt specific inhibitor wortmannin attenuated miR-221-mediated BCNU resistance and prompted cell apoptosis. We propose that miR-221 regulated cell proliferation and BCNU resistance in glioma cells by targeting PI3-K/PTEN/Akt signaling axis. Our findings may provide a new potential therapeutic target for treatment of glioblastoma. © 2014 Japanese Society of Neuropathology.


SHANGHAI, Nov 2, 2016 /PRNewswire/ -- SPI Energy Co., Ltd. ("SPI Energy" or the "Company") (Nasdaq: SPI), a global provider of photovoltaic (PV) solutions for business, residential, government and utility customers and investors, today announced changes to the senior management team and board of directors of the Company (the "Board"), effective on October 29, 2016: Mr. Roger Dejun Ye has resigned as Executive Vice President in charge of the Company's solar business but will remain as a non-executive director of the Board; Mr. Minghua Zhao, who currently serves as Joint Chief Operating Officer ("COO") of the Company's China domestic business, has been appointed as a director to the Board; and Mr. Fei Yun, who previously served as General Manager of Xinghang PV Technology (Suzhou) Co., Ltd., has joined the Company as Senior Vice President in charge of R&D and Solar Technology Development. "I would like to thank Roger for his contributions to the development of Company's solar business during his tenure and we look forward to continuing to work closely with Roger in his role as a director of the Board. On behalf of the management team and the Board, I also would like to extend our warm welcome to Minghua in joining the Board and Fei in joining the Company," said Xiaofeng Peng, Chairman and Chief Executive Officer of SPI Energy. Mr. Minghua Zhao currently serves as Joint COO of the Company's China domestic business and previously served as Senior Vice President of the Company's finance service business between February 2015 and June 2016. Before he joined the Company in February 2015, Mr. Zhao served as general manager of Suzhou Industrial Park Chengcheng Enterprises Guarantee Co., Ltd., a financial services company, and from 2003 to 2009 as president of Suzhou Industrial Park Branch of Suzhou Bank. Prior to that, he worked at CITIC Bank for six years. Mr. Zhao graduated from Jiangsu Province Business School in 1997 with a degree in Business Administration and from Southwestern University of Finance and Economics in 2008 with a degree in Business Management. Mr. Fei Yun has more than 30 years of experience in the research and development of solar cells, PV systems and senior management role in the industry in Australia and China. Mr. Fei Yun joined us from Xinghang PV Technology (Suzhou) Co., Ltd. where he has served as General Manager since July 2014. Previously, Mr. Yun held senior management positions at various solar companies, including as Vice President of Technology at LDK Solar Co., Ltd. from February 2010 to June 2013; Chief Technology Officer at Solar Enertech Corp. from December 2007 to January 2010; Vice President of Technology at SolarFun (Now Hanwha Solar One) from July 2006 to November 2007; General Manager and Chief Engineer at Tera Solar Technologies from March 2004 to June 2006. Mr. Yun received his bachelor's degree in Physics from Jinan University, his master's degree in Solar Energy from the Asian Institute of Technology(AIT) in Bangkok, Thailand. He also had nearly 10 years of research and development experience in silicon-based solar cells at the ARC Photovoltaics Centre of Excellence at the University of New South Wales in Sydney, Australia, his expertise is focused on the high efficiency silicon solar cell. About SPI Energy Co., Ltd. SPI Energy Co., Ltd. is a global provider of photovoltaic (PV) solutions for business, residential, government and utility customers and investors. SPI Energy focuses on the downstream PV market including the development, financing, installation, operation and sale of utility-scale and residential solar power projects in China, Japan, Europe and North America. The Company operates an innovative online energy e-commerce and investment platform, www.solarbao.com, which enables individual and institutional investors to purchase innovative PV-based investment and other products; as well as www.solartao.com, a B2B e-commerce platform offering a range of PV products for both upstream and downstream suppliers and customers. The Company has its operating headquarters in Shanghai and maintains global operations in Asia, Europe, North America and Australia. For additional information, please visit: www.spisolar.com, www.solarbao.com or www.solartao.com. This release contains certain "forward-looking statements." These statements are forward-looking in nature and subject to risks and uncertainties that may cause actual results to differ materially. All forward-looking statements included in this release are based upon information available to the Company as of the date of this release, which may change, and the Company undertakes no obligation to update or revise any forward-looking statements, except as may be required under applicable securities law. For investors and media inquiries please contact: To view the original version on PR Newswire, visit:http://www.prnewswire.com/news-releases/spi-energy-co-ltd-announces-new-director-and-management-appointments-to-strengthen-its-board-and-executive-leadership-300355730.html


Chen Z.-Y.,Jinan University | Nie P.-Y.,Guangdong University of Finance
Applied Energy | Year: 2016

Almost all countries around the world concern about the emission of greenhouse gas. The most widely used model of carbon tax is based on complete competition model of energy market, which, however, cannot completely reflect the real condition. This article establishes a social optimal welfare model that based on oligopoly competition of energy department. According to the model, the article calculates the substitution elasticity of factors in China's energy sectors, non-energy department and consumption preference in domestic energy and non-energy commodities. Based on the social optimal welfare model, the effect on social welfare caused by carbon taxes in different links is further evaluated. The results show that a certain amount of carbon tax in the production link raises the social welfare, while in consumption and redistribution links lowers the social welfare. Specifically, the absolute value of marginal social welfare in the redistribution link is larger. Moreover, the values of the three types of carbon taxes vary under different redistribution demands though the variation trends of tax in the same link are similar. As a result, a small amount of carbon tax on the production link contributes to the growth of social welfare. © 2016 Elsevier Ltd


Guo T.,Jinan University | Shang L.,Jinan University | Ran Y.,Jinan University | Guan B.-O.,Jinan University | Albert J.,Carleton University
Optics Letters | Year: 2012

A directional vibration sensor based on polarization-controlled cladding-to-core recoupling is demonstrated. A compact structure in which a short section of multi-mode fiber (MMF) stub containing a weakly tilted fiber Bragg grating (TFBG) is spliced to another single-mode fiber without any lateral offset. Multiple core modes of theMMFare coupled at the junction and appear as well defined resonances in reflection from the TFBG. Some of those resonances exhibit a strong polarization and bending dependence. Both the orientation and the amplitude of the vibrations can be determined unambiguously via dual-path power detection of the orthogonal-polarimetric lowest order LP1n modes. Meanwhile, the unwanted power fluctuations and temperature perturbations can be referenced out by monitoring the fundamental LP01 mode resonance. © 2012 Optical Society of America.


Cai Z.,Jinan University | Liu F.,Jinan University | Guo T.,Jinan University | Guan B.-O.,Jinan University | And 2 more authors.
Optics Express | Year: 2015

A novel tip-reflective and power-referenced refractometer based on strong fiber-to-fiber optical coupling for a large range of surrounding refractive index (SRI) (from 1.33 to 1.45) is proposed and experimentally demonstrated. A short D-shaped fiber stub is placed in parallel and close contact to another standard circular fiber containing a weakly tilted Bragg grating (TFBG). The TFBG couples the light from the circular fiber's core into its cladding where it remains guided. Apart from the direct light coupling over the contact interface, the evanescent field from the guided cladding modes penetrates the surroundings and reaches the D-fiber core by tunneling across the medium into which the fiber pair is located. The amount of tunneling depends strongly on the SRI so that the total amount of light collected by the D-fiber provides a measure of the SRI. Sensitivities ranging from ∼1000 to 13000 nW/RIU (Refractive Index Unit) have been obtained and the result is independent of temperature (within +/-10 nW of uncertainty). The measurement can be temperature-referenced through measurement of the TFBG spectrum if needed. © 2015 Optical Society of America.


Guo T.,Jinan University | Liu F.,Jinan University | Guan B.-O.,Jinan University | Albert J.,Carleton University
Optics Express | Year: 2014

The polarimetric sensing characteristics of multi-mode-fiber based tilted fiber Bragg gratings (MMF-TFBG) have been analyzed and experimentally demonstrated. The larger diameter fiber core and graded index core/cladding profile enable the tilted gratings to excite multiple highorder core modes with significantly different polarization dependence and to form a well-defined "comb" of spectrally separated resonances at different wavelengths. Orientation-recognized twist/rotation measurements (90° to 90°) have been achieved with sensitivity of 0.075 dB/deg by using intensity monitoring of two orthogonally polarized odd core-modes (LP11 and LP12). The proposed sensor is compact, works in reflection (a short section of MMF-TFBG spliced with a leading-in single mode fiber without any offset or tapering), is insensitive to temperature (intensity detection instead of wavelength monitoring) and is immune to unwanted intensity fluctuations (differential intensity measurement). Other TFBG sensing modalities, such as lateral pressure and surrounding refractive index are demonstrated separately with the same device configuration and interrogation principles. © 2014 Optical Society of America.


Guo T.,Jinan University | Liu F.,Jinan University | Du F.,Jinan University | Zhang Z.,Jinan University | And 3 more authors.
Optics Express | Year: 2013

A compact fiber-optic vector rotation sensor in which a short section of polarization-maintaining (PM) fiber stub containing a straight fiber Bragg grating (FBG) is spliced to another single mode fiber without any lateral offset is proposed and experimentally demonstrated. Due to the intrinsic birefringence of the PM fiber, two well-defined resonances (i.e. orthogonally polarized FBG core modes) with wavelength separation of 0.5 nm have been achieved in reflection, and they exhibit a high sensitivity to fiber rotation. Both the orientation and the angle of rotation can be determined unambiguously via simple power detection of the relative amplitudes of the orthogonal core reflections. Meanwhile, instead of using a broadband source (BBS), the sensor is powered by a commercial vertical cavity surface emitting laser (VCSEL) with the laser wavelength matched to the PM-FBG core modes, which enables the sensor to work at much higher power levels (̃15 dB better than BBS). This improves the signal-tonoise ratio considerably (̃50 dB), and makes a demodulation filter unnecessary. Vector rotation measurement with a sensitivity of 0.09 dB/deg has been achieved via cost-effective single detector real time power measurement, and the unwanted power fluctuations and temperature perturbations can be effectively referenced out. © 2013 Optical Society of America.


Caucheteur C.,University of Mons | Guo T.,Jinan University | Albert J.,Carleton University
Analytical and Bioanalytical Chemistry | Year: 2015

This paper presents a brief overview of the technologies used to implement surface plasmon resonance (SPR) effects into fiber-optic sensors for chemical and biochemical applications and a survey of results reported over the last ten years. The performance indicators that are relevant for such systems, such as refractometric sensitivity, operating wavelength, and figure of merit (FOM), are discussed and listed in table form. A list of experimental results with reported limits of detection (LOD) for proteins, toxins, viruses, DNA, bacteria, glucose, and various chemicals is also provided for the same time period. Configurations discussed include fiber-optic analogues of the Kretschmann–Raether prism SPR platforms, made from geometry-modified multimode and single-mode optical fibers (unclad, side-polished, tapered, and U-shaped), long period fiber gratings (LPFG), tilted fiber Bragg gratings (TFBG), and specialty fibers (plastic or polymer, microstructured, and photonic crystal fibers). Configurations involving the excitation of surface plasmon polaritons (SPP) on continuous thin metal layers as well as those involving localized SPR (LSPR) phenomena in nanoparticle metal coatings of gold, silver, and other metals at visible and near-infrared wavelengths are described and compared quantitatively.[Figure not available: see fulltext.] © 2015 Springer-Verlag Berlin Heidelberg


Wang Y.,Capital Medical University | Zhao X.,Capital Medical University | Liu L.,Capital Medical University | Wang D.,Illinois College | And 12 more authors.
New England Journal of Medicine | Year: 2013

BACKGROUND: Stroke is common during the first few weeks after a transient ischemic attack (TIA) or minor ischemic stroke. Combination therapy with clopidogrel and aspirin may provide greater protection against subsequent stroke than aspirin alone. METHODS: In a randomized, double-blind, placebo-controlled trial conducted at 114 centers in China, we randomly assigned 5170 patients within 24 hours after the onset of minor ischemic stroke or high-risk TIA to combination therapy with clopidogrel and aspirin (clopidogrel at an initial dose of 300 mg, followed by 75 mg per day for 90 days, plus aspirin at a dose of 75 mg per day for the first 21 days) or to placebo plus aspirin (75 mg per day for 90 days). All participants received open-label aspirin at a clinician-determined dose of 75 to 300 mg on day 1. The primary outcome was stroke (ischemic or hemorrhagic) during 90 days of follow-up in an intention-to-treat analysis. Treatment differences were assessed with the use of a Cox proportional-hazards model, with study center as a random effect. RESULTS: Stroke occurred in 8.2% of patients in the clopidogrel-aspirin group, as compared with 11.7% of those in the aspirin group (hazard ratio, 0.68; 95% confidence interval, 0.57 to 0.81; P<0.001). Moderate or severe hemorrhage occurred in seven patients (0.3%) in the clopidogrel-aspirin group and in eight (0.3%) in the aspirin group (P = 0.73); the rate of hemorrhagic stroke was 0.3% in each group. CONCLUSIONS: Among patients with TIA or minor stroke who can be treated within 24 hours after the onset of symptoms, the combination of clopidogrel and aspirin is superior to aspirin alone for reducing the risk of stroke in the first 90 days and does not increase the risk of hemorrhage. Copyright © 2013 Massachusetts Medical Society.


Guo T.,Jinan University | Liu F.,Jinan University | Liu Y.,Jinan University | Chen N.-K.,National United University | And 2 more authors.
Biosensors and Bioelectronics | Year: 2014

Tilted fiber Bragg grating (TFBG) biosensors can be used as a cost-effective and relatively simple-to-implement alternative to well established biosensor platforms for high sensitivity biological sample measurements in situ or possibly in vivo. The fiber biosensor presented in this study utilizes an in-fiber 12° tilted Bragg grating to excite a strong evanescent field on the surface of the sensor over a large range of external medium refractive indices. The devices have minimal cross-sensitivity to temperature and their fabrication does not impact the structural integrity of the fiber and its surface functionalization. Human acute leukemia cells with different intracellular densities and refractive index (RI) ranging from 1.3342 to 1.3344 were clearly discriminated in-situ by using the differential transmission spectrum between two orthogonal polarizations for the last guided mode resonance before "cut-off", with an amplitude variation sensitivity of 1.8×104dB/RIU, a wavelength shift sensitivity of 180nm/RIU, and a limit of detection of 2×10-5RIU. The detection process was precisely controlled with a micro-fluidic chip which allows the measurement of nL-volumes of bio-samples. The proposed in-fiber polarimetric biosensor is an appealing solution for rapid, sub-microliter dose and highly sensitive detection of analytes at low concentrations in medicine, chemical and environmental monitoring. © 2014 Elsevier B.V.


Su M.,Chinese University of Hong Kong | Chung H.Y.,Chinese University of Hong Kong | Li Y.,Jinan University | Li Y.,TCM and New Drug Research
Biochemical and Biophysical Research Communications | Year: 2011

Deoxyelephantopin (ESD), a naturally occurring sesquiterpene lactone present in the Chinese medicinal herb, Elephantopus scaber L. exerted anticancer effects on various cultured cancer cells. However, the cellular mechanisms by which it controls the development of the cancer cells are unavailable, particularly the human nasopharyngeal cancer CNE cells. In this study, we found that ESD inhibited the CNE cell proliferation. Cell cycle arrest in S and G2/M phases was also found. Western blotting analysis showed that modulation of cell cycle regulatory proteins was responsible for the ESD-induced cell cycle arrest. Besides, ESD also triggered apoptosis in CNE cells. Dysfunction in mitochondria was found to be associated with the ESD-induced apoptosis as evidenced by the loss of mitochondrial membrane potential (ΔΨm), the translocation of cytochrome c, and the regulation of Bcl-2 family proteins. Despite the Western blotting analysis showed that both intrinsic and extrinsic apoptotic pathways (cleavage of caspases-3, -7, -8, -9, and -10) were triggered in the ESD-induced apoptosis, additional analysis also showed that the induction of apoptosis could be achieved by the caspase-independent manner. Besides, Akt, ERK and JNK pathways were found to involve in ESD-induced cell death. Overall, our findings provided the first evidence that ESD induced cell cycle arrest, and apoptosis in CNE cells. ESD could be a potential chemotherapeutic agent in the treatment of nasopharyngeal cancer (NPC). © 2011 Elsevier Inc.


Sun P.,Hainan University | Sun P.,Jinan University | Nie P.-Y.,Jinan University
Renewable Energy | Year: 2015

By establishing a two-stage model in which a monopolization firm first chooses R&D input and then the quantity of energy, this paper compares the different effects of two regulatory policies: feed-in tariff (FIT) and renewable portfolio standard (PRS). The results show that FIT is more efficient than RPS to increase the quantity of renewable energy (installed capacity) and to stimulate the R&D input to reduce costs. And RPS policy is more efficient to reduce the carbon emissions and to improve the consumer surplus. Apart from existing findings, we cannot obtain the accurate conclusion about the effects of social welfare under the two policies. The effects of two policies on the social welfare heavily depend on the level of negative externality. © 2014 Elsevier Ltd.


Song T.-T.,Beijing University of Posts and Telecommunications | Song T.-T.,University of Toronto | Wen Q.-Y.,Beijing University of Posts and Telecommunications | Guo F.-Z.,Beijing University of Posts and Telecommunications | Tan X.-Q.,Jinan University
Physical Review A - Atomic, Molecular, and Optical Physics | Year: 2012

The length of signal pulses is finite in practical quantum key distribution. The finite-key analysis of an unconditional quantum key distribution is a burning problem, and the efficient quantum key distribution protocol suitable for practical implementation, measurement-device-independent quantum key distribution (MDI QKD), was proposed very recently. We give the finite-key analysis of MDI QKD, which removes all detector side channels and generates many orders of key rate higher than that of full-device-independent quantum key distribution. The secure bound of the ultimate key rate is obtained under the statistical fluctuations of relative frequency, which can be applied directly to practical threshold detectors with low detection efficiency and highly lossy channels. The bound is evaluated for reasonable values of the observed parameters. The simulation shows that the secure distance is around 10 km when the number of sifted data is 1010. Moreover the secure distance would be much longer in practice because of some simplified treatments used in our paper. © 2012 American Physical Society.


Liu S.,Jinan University | Yang X.,Shanghai JiaoTong University
Archive for Rational Mechanics and Analysis | Year: 2016

Boundary effects are central to the dynamics of the dilute particles governed by the Boltzmann equation. In this paper, we study both the diffuse reflection and the specular reflection boundary value problems for the Boltzmann equation with a soft potential, in which the collision kernel is ruled by the inverse power law. For the diffuse reflection boundary condition, based on an L2 argument and its interplay with intricate (Formula presented.) analysis for the linearized Boltzmann equation, we first establish the global existence and then obtain the exponential decay in (Formula presented.) space for the nonlinear Boltzmann equation in general classes of bounded domain. It turns out that the zero lower bound of the collision frequency and the singularity of the collision kernel lead to some new difficulties for achieving the a priori (Formula presented.) estimates and time decay rates of the solution. In the course of the proof, we capture some new properties of the probability integrals along the stochastic cycles and improve the (Formula presented.) theory to give a more direct approach to overcome those difficulties. As to the specular reflection condition, our key contribution is to develop a new time-velocity weighted (Formula presented.) theory so that we could deal with the greater difficulties stemming from the complicated velocity relations among the specular cycles and the zero lower bound of the collision frequency. From this new point, we are also able to prove that the solutions of the linearized Boltzmann equation tend to equilibrium exponentially in (Formula presented.) space with the aid of the L2 theory and a bootstrap argument. These methods, in the latter case, can be applied to the Boltzmann equation with soft potential for all other types of boundary condition. © 2016 Springer-Verlag Berlin Heidelberg


Xiao L.-J.,Jinan University | Wang T.,Jinan University | Hu R.,Jinan University | Han B.-P.,Jinan University | And 3 more authors.
Water Research | Year: 2011

Liuxihe reservoir is a deep, monomictic, oligo-mesotrophic canyon-reservoir in the subtropical monsoon climate region of southern China. Phytoplankton functional groups in the reservoir were investigated and a comparison made between the succession observed in 2008, an exceptionally wet year, and 2009, an average year. The reservoir shows strong annual fluctuations in water level caused by monsoon rains and artificial drawdown. Altogether 28 functional groups of phytoplankton were identified, including 79 genera. Twelve of the groups were analyzed in detail using redundancy analysis. Because of the oligo-mesotrophic and P-limited condition of the reservoir, the dominant functional groups were those tolerant of nutrient (phosphorus) deficiency. The predominant functional groups in the succession process were Groups A (Cyclotella with greatest axial linear dimension < 10 μm), B (Cyclotella with greatest axial linear dimension >10 μm), L O (Peridinium), L M (Ceratium and Microcystis), E (Dinobryon and Mallomonas), F (Botryococcus), X 1 (Ankistrodesmus, Ankyra, Chlorella and Monoraphidium) and X 2 (Chlamydomonas and Chroomonas). The development of groups A, B and L O was remarkably seasonal. Group A was dominant during stratification, when characteristic small size and high surface/volume ratio morphology conferred an advantage. Group L O was dominant during dry stratification, when motility was advantageous. Group B plankton exhibited a high relative biomass during periods of reduced euphotic depth and isothermy. Groups L M, E, F, X 1 and X 2 occasionally exhibited high relative biomasses attributable to specific environmental events (e.g. drawdown, changes in zooplankton community). A greater diversity of phytoplankton functional groups was apparent during isothermy. This study underscores the usefulness of functional algal groups in studying succession in subtropical impoundments, in which phytoplankton succession can be significantly affected by external factors such as monsoonal hydrology and artificial drawdown, which alter variables such as retention time, mixing regime and thermal structure and influence light and nutrient availability. © 2011 Elsevier Ltd.


Deng J.,China National Analytical Center Guangzhou | Fan C.,Jinan University | Yang Y.,China National Analytical Center Guangzhou
Journal of Pharmaceutical and Biomedical Analysis | Year: 2011

Deng's herbal tea (DHT), a famous traditional Chinese herbal tea consisting of six traditional Chinese medicines (Honeysuckle, Chrysanthemum, Rhizoma imperatae, Folium mori, dandelion and liquorice), is widely used in China for its health benefits. In this paper, a rapid resolution liquid chromatography coupled with mass spectrometry (RRLC-MS) method was developed for the identification and determination of the major constituents in DHT granules. A good RRLC separation was achieved using an Agilent Poroshell 120 SB-C 18 column and gradient elution (0.5% formic acid in water/acetonitrile) within 30min. Twenty-eight compounds were identified or tentatively characterized based on their exact molecular weights and fragmentation patterns. Fifteen major bioactive constituents of those 28 compounds were chosen as the benchmark substances. Their quantitative analyses were performed by a triple quadrupole tandem mass spectrometer (MS/MS) operating in multiple-reaction monitoring mode, and a full quantitative analysis of the 15 major constituents was performed by our developed RRLC-MS/MS method in only 10min. Of the 16 DHT granule samples tested, the quality of the results was stable, which confirms that the developed method was efficient and robust for the quality control of DHT granules. © 2011 Elsevier B.V.


Ke Y.,Jinan University | Ke Y.,South China University of Technology | Wang Y.J.,South China University of Technology | Ren L.,South China University of Technology | And 2 more authors.
Acta Biomaterialia | Year: 2010

An ideal scaffold provides an interface for cell adhesion and maintains enough biomechanical support during tissue regeneration. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds with pore sizes ranging from 100 to 500 μm and porosity ∼90% were prepared by the particulate-leaching method, and then modified by the introduction of polyacrylamide (PAM) on the inner surface of scaffolds using in situ UV polymerization, with the aim of enhancing the biological and mechanical properties of the PHBV scaffolds. The modified PHBV scaffolds had interconnected pores with porosity of 75.4-78.6% and pore sizes at peak volume from 20 to 50 μm. The compressive load and modulus were up to 62.45 N and 1.06 MPa, respectively. The water swelling percentage (WSP) of the modified PHBV scaffolds increased notably compared with that of the PHBV scaffolds, with the maximum WSP at 537%. Sheep bone mesenchymal stem cells (BMSC) were cultured on the PHBV and modified PHBV. The hydrophilic PAM chains did not influence BMSC viability or proliferation index, but the initial cell adhesion at 1 h of culture was enhanced significantly. Framing PHBV scaffold along with gel-like PAM chains inside is a novel model of inner surface modification for PHBV scaffolds, which shows potential in tissue engineering applications. © 2009 Acta Materialia Inc.


Chen Z.,North China University of Water Conservancy and Electric Power | Yu L.,Jinan University
Zhendong yu Chongji/Journal of Vibration and Shock | Year: 2014

Aiming at ill-posed problems of solving moving force identification system matrix, a truncated generalized singular value decomposition(TGSVD) algorithm for moving force identification was developed by introducing regularization matrix. The numerical simulation of two-axle moving force loads showed that the proposed method TGSVD has many good properties compared with the time domain method(TDM), such as, higher identification accuracy, robust noise immunity and less effect of response type and response combination on its recognition results, it is beneficial to the application of TGSVD in field identification of dynamic axle loads on a bridge.


Liu X.,Jinan University | Xiang J.-J.,Jinan University | Tang Y.,Jinan University | Tang Y.,Pacific Northwest National Laboratory | And 4 more authors.
Analytica Chimica Acta | Year: 2012

An immunochromatographic assay (ICA) using gold nanoparticles coated with monoclonal antibody (McAb) for the detection of chromium ions (Cr) in water and serum samples was developed, optimized and validated. Gold nanoparticles coated with affinity-purified monoclonal antibodies against isothiocyanobenzyl-EDTA (iEDTA)-chelated Cr3+ were used as the detecting reagent in this completive immunoassay-based one-step test strip. The ICA was investigated to measure chromium speciation (Cr3+ and Cr6+ ions) in water samples. Chromium standard samples of 0-80ngmL-1 in water were determined by the test strips. The results showed that the visual lowest detection limit (LDL) of the test strip was 50.0ngmL-1. A portable colorimetric lateral flow reader was used for the quantification of Cr. The results indicated that the linear range of the ICA with colorimetric detection was 5-80ngmL-1. The ICA was also validated for the detection of chromium ions in serum samples. The test trips showed high stability in that they could be stored at 37°C for at least 12 weeks without significant loss of activity. The test strip also showed good selectivity for Cr detection with negligible interference from other heavy metals. Because of its low cost and short testing time (within 5min), the test strip is especially suitable for on-site large-scale screening of Cr-polluted water samples, biomonitoring of Cr exposure, and many other field applications. © 2012 Elsevier B.V.


Xi B.,Shandong University | Takeuchi F.,National Health Research Institute | Chandak G.R.,Center for Cellular and Molecular Biology | Kato N.,National Health Research Institute | And 4 more authors.
Diabetologia | Year: 2012

Aims/hypothesis: Genome-wide association studies have shown that variants near the melanocortin 4 receptor gene (MC4R) (rs17782313 and rs12970134) are associated with risk of obesity in Europeans. As obesity is associated with an increased risk of type 2 diabetes, many studies have investigated the association between polymorphisms near the MC4R gene and type 2 diabetes risk across different ethnic populations, with inconsistent results. In this study, we performed a meta-analysis to clarify the association of variants near MC4R with type 2 diabetes risk. Methods: Published literature from PubMed and Embase was retrieved. All studies that evaluated the association of at least one of the two MC4R polymorphism(s) with type 2 diabetes were included in the study. Pooled ORs with 95% CIs were calculated using the fixed-effects model. Results: A total of 19 studies (comprising 34,195 cases and 89,178 controls) of the rs17782313 polymorphism (or its proxy rs12970134) were included in the meta-analysis. The results indicated that the rs17782313 polymorphism was significantly associated with type 2 diabetes risk among the overall study population (OR 1.10, 95% CI 1.07, 1.13, p∈=∈2. 83∈×∈10-12 [Z test], I 2∈=∈9.1%, p∈=∈0.345 [heterogeneity]). The association remained significant even after adjustment for body mass index (BMI) (OR 1.06, 95% CI 1.03, 1.09, p∈=∈2.14∈×∈10-5 [Z test], I 2∈=∈4.9%, p∈=∈0.397 [heterogeneity]). Further sensitivity analysis confirmed the statistically significant association of rs17782313 polymorphism with type 2 diabetes, and no publication bias was detected. Conclusions/interpretation: The present meta-analysis confirmed the significant association of the rs17782313 polymorphism near the MC4R gene with type 2 diabetes risk, which was independent of BMI. © 2012 Springer-Verlag.


Hemberger Y.,University of Würzburg | Xu J.,Heinrich Heine University Düsseldorf | Xu J.,Hainan University | Wray V.,Helmholtz Center for Infection Research | And 3 more authors.
Chemistry - A European Journal | Year: 2013

From the endophytic fungus Pestalotiopsis sp. isolated from the leaves of the Chinese mangrove, Rhizophora mucronata, two novel hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon skeleton, named pestalotiopensA and B, were obtained, together with the already known phytotoxin altiloxinB. PestalotiopenB even contains a third, triketide-derived module. The constitutions and the absolute configurations of the new metabolites and of altiloxinB were unambiguously determined by a combination of spectroscopic methods and quantum-chemical optical-rotatory dispersion (ORD) and circular dichroism (CD) calculations. A biosynthetic pathway to pestalotiopensA and B is proposed with altiloxinB as one of the suggested precursors. PestalotiopenA shows moderate antimicrobial activity against Enterococcus faecalis. A stereochemical challenge! PestalotiopensA and B, isolated from the Chinese mangrove fungus Pestalotiopsis sp., are novel hybrid compounds. The conformational and stereochemical behavior of these highly flexible compounds and also of altiloxinB was thoroughly investigated, and their absolute configurations were successfully elucidated by the combination of spectroscopic methods and quantum-chemical calculations (see figure). Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Jiang Z.,Jinan University | Smith N.W.,King's College London | Liu Z.,Jinan University
Journal of Chromatography A | Year: 2011

Hydrophilic interaction chromatography (HILIC) has experienced increasing attention in recent years. Much research has been carried out in the area of HILIC separation mechanisms, column techniques and applications. Because of their good permeability, low resistance to mass transfer and easy preparation within capillaries, hydrophilic monolithic columns represent a trend among novel HILIC column techniques. This review attempts to present an overview of the preparation and applications of HILIC monolithic columns carried out in the past decade. The separation mechanism of various hydrophilic monolithic stationary phases is also reviewed. © 2011 Elsevier B.V.


Leung H.W.C.,Min Sheng General Hospital | Chan A.L.F.,Min Sheng General Hospital | Leung M.S.H.,Jinan University | Lu C.-L.,Chi Mei Medical Center
Annals of Pharmacotherapy | Year: 2013

Objective: To systematically review and assess the quality of cost-effectiveness analyses (CEAs) of pharmaceutical therapies for metastatic colorectal cancer (mCRC). Data sources: The MEDLINE, EMBASE, Cochrane, and EconLit databases were searched for the Medical Subject Headings or text key words quality-adjusted, QALY, life-year gained (LYG), and cost-effectiveness (January 1, 1999-December 31, 2009). Study selection: Original CEAs of mCRC pharmacotherapy published in English were included. CEAs that measured health effects in units other than quality-adjusted life years or LYG and letters to the editor, case reports, posters, and editorials were excluded. Data extraction: Each article was independently assessed by 2 trained reviewers according to a quality checklist created by the Panel on Cost-Effectiveness in Health and Medicine. Results: Twenty-four CEA studies pertaining to pharmaceutical therapies for mCRC were identified. All studies showed a wide variation in methodologic approaches, which resulted in a different range of incremental cost-effectiveness ratios reported for each regimen. We found common methodologic flaws in a significant number of CEA studies, including lack of clear description for critique of data quality; lack of method for adjusting costs for inflation and methods for obtaining expert judgment; no results of model validation; wide differences in the types of perspective, time horizon, study design, cost categories, and effect outcomes; and no quality assess ment of data (cost and effectiveness) for the interventions evaluation. Conclusions: This study has shown a wide variation in the methodology and quality of cost-effectiveness analysis for mCRC. Improving quality and harmonization of CEA for cancer treatment is needed. Further study is suggested to assess the quality of CEA methodology outside the mCRC disease state. © 1967-2013 Harvey Whitney Books Co. All rights reserved.


Wang J.,Shanghai JiaoTong University | Liu X.,Shanghai JiaoTong University | Wu H.,Shanghai JiaoTong University | Ni P.,Shanghai JiaoTong University | And 5 more authors.
Nucleic Acids Research | Year: 2010

Long non-coding RNA (lncRNA), highly up-regulated in liver cancer (HULC) plays an important role in tumorigenesis. Depletion of HULC resulted in a significant deregulation of several genes involved in liver cancer. Although up-regulation of HULC expression in hepatocellular carcinoma has been reported, the molecular mechanisms remain unknown. In this study, we used in vivo and in vitro approaches to characterize cancerdependent alterations in the chromatin organization and find a CREB binding site (encompassing from -67 to -53 nt) in the core promoter. Besides, we also provided evidence that PKA pathway may involved in up-regulation of HULC. Furthermore, we demonstrated HULC may act as an endogenous 'sponge', which down-regulates a series of microRNAs (miRNAs) activities, including miR-372. Inhibition of miR-372 leads to reducing translational repression of its target gene, PRKACB, which in turn induces phosphorylation of CREB. Over-expression of miR-372 decreases the association of CREB with the proximal promoter, followed by the dissociation of P300, resulting in a change of the histone 'code', such as in deacetylation and methylation. The study elucidates that fine tuning of HULC expression is part of an auto-regulatory loop in which it's inhibitory to expression and activity of miR-372 allows lncRNA up-regulated expression in liver cancer. © The Author(s) 2010. Published by Oxford University Press.


News Article | February 15, 2017
Site: www.eurekalert.org

New Rochelle, NY, February 14, 2017--A special issue on progress toward a cure for HIV includes a description of a previously unreported study started in the early 2000s that describes AIDS patients currently ages 51-67 in good health. These nine individuals were treated with a unique formula of traditional Chinese herbal medicine (TCM) from 2001-2006 or longer, with or without occasional antiviral therapy added later. The fact that the patients currently have low or undetectable HIV in their systems is unexpected and intriguing, and suggests a potential promise of TCM as a functional cure for HIV/AIDS, as discussed in a Letter to the Editor in the special issue of AIDS Research and Human Retroviruses, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. The Letter to the Editor is available open access on the AIDS Research and Human Retroviruses website. In "Long-Term Survival of AIDS Patients Treated with Only Traditional Chinese Medicine," Yifei Wang, Fujun Jin, Qiaoli Wang, and Zucai Suo, Jinan University (Guangdong, China) and The Ohio State University (Columbus), report that most of the individuals in this small study have undetectable viral loads, with one patient having a low viral load. Their CD4+ counts and CD4+/CD8+ ratios are all excellent. In an accompanying Editorial entitled "Can a Traditional Chinese Medicine Contribute to a Cure for HIV?" Thomas Hope, PhD, Editor-in-Chief of AIDS Research and Human Retroviruses and Professor of Cell and Molecular Biology at Northwestern University, Feinberg School of Medicine (Chicago, IL), while pointing out the limitations in interpreting the outcome of this small, non-placebo-controlled study, comments on the importance of putting "these observations into the hands of the HIV research community." He writes, "I believe there should be some effort to further explore this phenomenon." Both the Letter to the Editor and Editorial are part of a new Special Issue on HIV Cure Research published in AIDS Research and Human Retroviruses. AIDS Research and Human Retroviruses, published monthly online with open access options and in print, presents papers, reviews, and case studies documenting the latest developments and research advances in the molecular biology of HIV and SIV and innovative approaches to HIV vaccine and therapeutic drug research, including the development of antiretroviral agents and immune-restorative therapies. Content also explores the molecular and cellular basis of HIV pathogenesis and HIV/HTLV epidemiology. The Journal features rapid publication of emerging sequence information, reports on clinical trials of emerging HIV therapies, and images in HIV research. Tables of content and a sample issue may be viewed on the AIDS Research and Human Retroviruses website. Mary Ann Liebert, Inc., publishers is a privately held, fully integrated media company known for establishing authoritative peer-reviewed journals in many promising areas of science and biomedical research, including AIDS Patient Care and STDs, Viral Immunology, and Journal of Interferon and Cytokine Research. Its biotechnology trade magazine, GEN (Genetic Engineering & Biotechnology News), was the first in its field and is today the industry's most widely read publication worldwide. A complete list of the firm's 80 journals, books, and newsmagazines is available on the Mary Ann Liebert, Inc., publishers website.


Li B.,University of Hong Kong | Li B.,Center for Cancer Research | Tsao S.W.,University of Hong Kong | Tsao S.W.,Center for Cancer Research | And 8 more authors.
Clinical Cancer Research | Year: 2014

Purpose: To investigate the autocrine/endocrine role of Id1-induced insulin-like growth factor-II (IGF-II) in esophageal cancer, and evaluate the potential of IGF-II-and IGF-type I receptor (IGF-IR)-targeted therapies. Experimental Design: Antibody array-based screening was used to identify differentially secreted growth factors from Id1-overexpressing esophageal cancer cells. In vitro and in vivo assays were performed to confirm the induction of IGF-II by Id1, and to study the autocrine and endocrine effects of IGF-II in promoting esophageal cancer progression. Human esophageal cancer tissue microarray was analyzed for overexpression of IGF-II and its correlation with that of Id1 and phosphorylated AKT (p-AKT). The efficacy of intratumorally injected IGF-II antibody and intraperitoneally injected cixutumumab (fully human monoclonal IGF-IR antibody) was evaluated using in vivo tumor xenograft and experimental metastasis models. Results: Id1 overexpression induced IGF-II secretion, which promoted cancer cell proliferation, survival, and invasion by activating AKT in an autocrine manner. Overexpression of IGF-II was found in 21 of 35 (60%) esophageal cancer tissues and was associated with upregulation of Id1 and p-AKT. IGF-II secreted by Id1-overexpressing esophageal cancer xenograft could instigate the growth of distant esophageal tumors, as well as promote metastasis of circulating cancer cells. Targeting IGF-II and IGF-IR had significant suppressive effects on tumor growth and metastasis in mice. Cixutumumab treatment enhanced the chemosensitivity of tumor xenografts to fluorouracil and cisplatin. Conclusions: The Id1-IGF-II-IGF-IR-AKT signaling cascade plays an important role in esophageal cancer progression. Blockade of IGF-II/IGF-IR signaling has therapeutic potential in the management of esophageal cancer. © 2014 American Association for Cancer Research.


Leskinen H.M.,University of Turku | Suomela J.-P.,University of Turku | Kallio H.P.,University of Turku | Kallio H.P.,Jinan University
Rapid Communications in Mass Spectrometry | Year: 2010

The regioisomer composition of triacylglycerols (TAGs) in various vegetable oils was determined with a new liquid chromatography/tandem mass spectrometry (LC/MS/MS method). A direct inlet ammonia negative ion chemical ionization (NICI) MS/MS method was improved by adapting it to LC negative ion (NI) atmospheric pressure chemical ionization (APCI) MS/MS system using ammonia as nebulizer gas. The method is based on the preferential formation of [M-H-RCOOH-100] ions during collision-induced dissociation by loss of sn-1/3 fatty acids from [M-H] ions. Calibration curves were created from nine reference TAGs: Ala/L/L, Gla/L/L, L/L/O, L/O/O, P/O/O, P/P/O, Po/Po/ V, Po/Po/O, and C/O/O. The calibration curves were used to quantify the regioisomer compositions of selected TAGs in rapeseed oil, sunflower seed oil, palm oil, black currant seed oil, and sea buckthorn pulp oil. The method discriminates the different regioisomers and the results obtained by this method were in good agreement with previous results. This proves that this new method can be used for the determination of regiospecific distribution of fatty acids in TAGs. © 2009 John Wiley & Sons, Ltd.


Zhu Y.,Hong Kong University of Science and Technology | Wang H.,Yunnan University of Finance and Economics | Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
International Journal of Plasticity | Year: 2014

One of the main targets in the development of dislocation based continuum crystal plasticity theories is to establish continuum constitutive relations which approximately summarize the underlying discrete dislocation dynamics (DDD). However, rigorously transiting from discrete to continuum in describing the evolution of dislocation system is extremely challenging for complex networks of curved dislocations and their interactions at multiple length scales. To address this difficulty, a coarse-grained disregistry function (CGDF) was proposed to represent the continuous distributions of curved dislocations. In this paper, we present a dislocation based continuum model for crystal plasticity incorporating the Frank-Read sources, which serves as a crucial step towards systematically building a three-dimensional dislocation based continuum plasticity theory. The continuum model is derived accurately from the DDD model, and is validated by comparisons of the results with theoretical predictions and DDD simulations conducted under the same conditions. Furthermore by considering dislocation loop pileups within a rectangular grain, we derive analytical formulas which generalize the traditional Hall-Petch relation into two dimensions without any adjustable parameters. It is shown that the yield stress of a rectangular grain depends not only on the grain size, but also on the grain aspect ratio whose exact form is associated with the harmonic mean of the length and width of the rectangle. The derived formulas of the yield stress are shown in excellent agreement with the results by our continuum model and DDD simulations. © 2014 Elsevier Ltd. All rights reserved.


Zhu X.,Hong Kong University of Science and Technology | Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
Scripta Materialia | Year: 2011

In this paper, we study the glide force due to stress on the constituent dislocations of slightly perturbed symmetric low-angle tilt boundaries. We show that the stabilizing force comes from both the long-range interaction of the constituent dislocations and their local line tension effect. We also present a continuum model for this glide force. The obtained results and continuum model provide a basis for further understanding of the stress-driven migration of distorted grain boundaries. © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.


Yin G.Q.,Nanjing Southeast University | Zhong B.,Jinan University
Journal of Gastroenterology and Hepatology (Australia) | Year: 2016

Background and Aim: In China, inappropriate therapies with nucleos(t)ide analogues (NA) have induced hepatitis B virus resistance, combination therapy with nucleoside and nucleotide (ComTNsNt) failure, or multi-drug resistant mutations. However, the efficacy of combination therapy with entecavir plus tenofovir for ComTNsNt failure is limited. In the current study, the regimens of interferon-α (IFN-α) therapy, switching from NAs to IFN-α, and subsequent re-treatment with IFN-α were applied to treat ComTNsNt failure. We further evaluated the efficacy of this therapy. Methods: Eleven patients with ComTNsNt failure were enrolled in this study. Nine subjects (9/11) received IFN-α switching therapy. Combination therapy with IFN-α and ComTNsNt was administered in the first 4weeks. Then, ComTNsNt was discontinued at the end of Week 4, and IFN-α monotherapy was continued for 6months. Two (2/11) patients discontinued ComTNsNt without receiving IFN-α treatment. All 11 patients received the first re-treatment of IFN-α when they experienced hepatitis relapses after the withdrawal of IFN-α or ComTNsNt. Six (6/11) patients received a second re-treatment of IFN-α. Follow up was conducted after IFN-α therapy in all 11 patients. Results: Two patients (2/9) receiving IFN-α switching therapy experienced alanine aminotransferase (ALT) flare. In contrast, the two patients without IFN-α switching therapy experienced ALT flare. Multiple re-treatments with IFN-α resulted in a sustained response. Conclusions: Interferon-α switching therapy and IFN-α re-treatment might be applied for treatment of ComTNsNt failure. IFN-α switching therapy resulted in safe ComTNsNt cessation, and IFN-α re-treatment induced a sustained response of IFN-α in all patients. This IFN-α treatment is an optional treatment for ComTNsNt failure. © 2015 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.


Guo P.,Tissuetech | Guo P.,Jinan University | Zhang S.-Z.,Tissuetech | He H.,Tissuetech | And 2 more authors.
Investigative Ophthalmology and Visual Science | Year: 2012

PURPOSE. To investigate the role of anti-inflammatory TSG-6 in controlling MMP-1 and MMP-3, which have been shown to be upregulated in conjunctivochalasis (CCh). METHODS. Immunostaining of TSG-6 was compared between normal and CCh conjunctiva and Tenon's capsule. Second cultures of normal and CCh fibroblasts were transfected with or without TSG-6 siRNA and then with or without the addition of TNF-α or IL-1β. Cell lysates and culture media were collected to assess apoptosis with the use of ELISA and the expression of TSG-6, MMP-1, and MMP-3 transcripts and proteins with the use of qRT-PCR and Western blot analysis, respectively. RESULTS. TSG-6 expression was constitutive in the in vivo normal conjunctival epithelium. Significantly more TSG-6-positive cells than normal specimens were noted in CCh subconjunctival tissue and Tenon's capsule. TSG-6 was constitutively expressed intracellularly by both resting normal and CCh fibroblasts but was secreted extracellularly only by resting CCh fibroblasts. Intracellular and extracellular TSG-6 proteins were markedly upregulated by TNF-α or IL-1β in normal and CCh fibroblasts. Active MMP-1 was found in CCh fibroblasts intracellularly and extracellularly, whereas only proMMP-1 was found intracellularly in normal fibroblasts. Knockdown by TSG-6 siRNA upregulated more MMP-1 than MMP-3 transcripts in normal and CCh fibroblasts. TSG-6 siRNA led to extracellular MMP-1 expression by normal fibroblasts such as CCh fibroblasts. This activation of MMP-1 was further enhanced by IL-1β. Cell apoptosis was higher in CCh fibroblasts and further aggravated by TSG-6 siRNA knockdown. CONCLUSIONS. TSG-6 exerts an anti-inflammatory function by counteracting the transcription of MMP-1 and MMP-3 and the activation of MMP-1. Dysfunction of TSG-6 might play a role in the pathogenesis of CCh. © 2012 The Association for Research in Vision and Ophthalmology, Inc.


Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
Journal of the Mechanics and Physics of Solids | Year: 2014

We present a continuum framework for dislocation structure, energy and dynamics of dislocation arrays and low angle grain boundaries that are allowed to be nonplanar or nonequilibrium. In our continuum framework, we define a dislocation density potential function on the dislocation array surface or grain boundary to describe the orientation dependent continuous distribution of dislocations in a very simple and accurate way. The continuum formulations incorporate both the long-range dislocation interaction and the local dislocation line energy, and are derived from the discrete dislocation model. The continuum framework recovers the classical Read-Shockley energy formula when the long-range elastic fields of the low angle grain boundaries are canceled out. Applications of our continuum framework in this paper are focused on dislocation structures on static planar and nonplanar low angle grain boundaries and misfitting interfaces. We present two methods under our continuum framework for this purpose, including the method based on the Frank×3.


Zhu X.,Jinan University | Dai S.,Hong Kong University of Science and Technology | Xiang Y.,Hong Kong University of Science and Technology
International Journal of Plasticity | Year: 2013

We present a simulation method for the dynamics of dislocation arrays. In this numerical method, dislocation arrays are considered as continuous surfaces in three dimensions, and the level set representation is used for these dislocation array surfaces. The level set representation of the surfaces has the advantage of automatically handling the topological changes occurring during the evolution, and simple implementation using standard accurate finite difference schemes on a uniform grid. The driving force of the evolution of the dislocation array surfaces comes from both the long-range interaction of the constituent dislocations and their local curvature effect. The long-range interaction, which is expressed by a complicated integral over the whole dislocation array surface, is calculated efficiently using the fast Fourier transform (FFT) method. Simulations are performed for dislocation arrays bypassing different particles under applied stress and are compared with those of a single dislocation. The long-range nature of the stress fields of nonplanar infinite dislocation arrays is discussed, and is shown to be essentially different from that by a single dislocation. Examples also show that such long-range stress fields may contribute to the formation of inhomogeneities of dislocation distributions. © 2012 Elsevier Ltd. All rights reserved.


Yuan H.,Jinan University | Lu X.,Jinan University | Hui D.,University of New Orleans | Feo L.,University of Salerno
Composite Structures | Year: 2012

This latest experimental study proposes a theory that the bond-slip law for a FRP-concrete interface contains linear hardening and exponential softening. On the basis of this law, the paper studies the mechanic behavior and debonding process of a FRP-concrete interface. Firstly, through nonlinear fracture mechanics, the analytical solutions of the interface shear stress, the axial normal stress of FRP and the load-displacement relationship at the loaded end with the single shear test model of FRP-concrete are acquired. The shear stress propagation as well as the debonding process of the whole interface for different bond lengths could be predicted. Secondly, a simplified interface bond-slip law is used by changing the exponential softening law into a linear softening law. In addition, the analytical solutions for the simplified interface bond-slip law could also be obtained. Finally, based on the analytical solutions of the two bond-slip laws, the influences of the FRP bond length and stiffness on load-displacement curve and the ultimate load, as well as stiffness on effective bond length were discussed, with the similarities and differences between the two bond-slip laws also being studied. © 2012 Elsevier Ltd.


Liu P.,University of Turku | Kallio H.,University of Turku | Kallio H.,Jinan University | Yang B.,University of Turku | Yang B.,Jinan University
Journal of Agricultural and Food Chemistry | Year: 2011

Phenolics in the fruits and leaves of Crataegus grayana were identified by HPLC-UV-ESI-MS. The contents of these compounds and their changes during autumn were also analyzed. Epicatechin [1-7 mg/g dry mass (DM) in fruits and 1-10 mg/g DM in leaves), procyanidins B2 (2-4 and 1-8 mg/g DM) and C1 (2-4 and 1-8 mg/g DM), hyperoside (0.5-1 and 2-11 mg/g DM), and a quercetin-pentoside (0.3-0.5 and 2-6 mg/g DM) were the major phenolics in both fruits and leaves. C-Glycosyl flavones were present in leaves (2-5 mg/g DM), whereas only trace levels were found in fruits. Ideain and 5-O-caffeoylquinic acid were found only in fruits. An additional 11 phenolics were identified/tentatively identified. Total phenolic contents reached highest levels by the end of August in fruits and by the end of September in leaves. The compositional profiles of phenolics in fruits and leaves of C. grayana were different from those of other Crataegus species. © 2011 American Chemical Society.


Wang J.,Anhui University of Science and Technology | Zhou Z.,Anhui University of Science and Technology | Zhou Z.,Monash University | Zhang W.,Jinan University | And 2 more authors.
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2013

We simulate the bond and site percolation models on a simple-cubic lattice with linear sizes up to L=512, and estimate the percolation thresholds to be pc(bond)=0.24881182(10) and pc(site)=0.3116077(2). By performing extensive simulations at these estimated critical points, we then estimate the critical exponents 1/ν=1.1410(15), β/ν=0.47705(15), the leading correction exponent yi=-1.2(2), and the shortest-path exponent dmin=1.3756(3). Various universal amplitudes are also obtained, including wrapping probabilities, ratios associated with the cluster-size distribution, and the excess cluster number. We observe that the leading finite-size corrections in certain wrapping probabilities are governed by an exponent ≈-2, rather than yi≈-1.2. © 2013 American Physical Society.


Su H.,Hong Kong University of Science and Technology | Zhong Y.,Hong Kong University of Science and Technology | Zhong Y.,Jinan University | Ming T.,Chinese University of Hong Kong | And 2 more authors.
Journal of Physical Chemistry C | Year: 2012

Plasmon-coupled fluorescence from Au nanorods with fluorophore-doped silica shells has been studied. Besides the ordinary emission of the fluorophore at 590 nm, an extra emission peak was found on the normally weak fluorescence tail at the surface plasmon resonance (SPR) of the Au nanorods around 750 nm. It provides clear evidence of the plasmonic emission. Our experiment results suggest that the linear absorption coefficient of fluorophores was strongly enhanced around the SPR wavelength and the plasmonic emission at 750 nm was created by the resonance energy transfer from the excited fluorophores to SPRs. The energy transfer rate was estimated to be 5.22 ns -1, and the total quantum yield of the sample was 1.5 times that of the free fluorophore molecules in aqueous solutions. This study supports that strong fluorescence at desired wavelength can be engineered by fluorophore-plasmon coupling. © 2012 American Chemical Society.


Ou J.,Jinan University | Qi X.,Hong Kong University of Science and Technology | Lee C.-Y.,Hong Kong University of Science and Technology
Journal of Scheduling | Year: 2010

We study a parallel machine scheduling problem with multiple unloading servers. After a machine completes processing one job, an unloading server is needed to remove the job from the machine. Only after unloading, the machine is available for processing the next job. The model is motivated by the milk run operations of a logistics company that faces limited unloading docks at the warehouse. Our interest is to minimize the total completion time of the jobs. We show that the shortest-processing-time-first (SPT) algorithm has a worst-case bound of 2. We also develop other improved heuristic algorithms as well as a branch-and-bound algorithm to solve the problem. Computational experiments show that our algorithms are efficient and effective. © 2009 Springer Science+Business Media, LLC.


Liu P.,University of Turku | Kallio H.,University of Turku | Kallio H.,Jinan University | Lu D.,ShenYang Agricultural University | And 2 more authors.
Food Chemistry | Year: 2011

Eleven major phenolic compounds (hyperoside, isoquercitrin, chlorogenic acid, ideain, epicatechin, two procyanidin (PA) dimers, three PA trimers and a PA dimer-hexoside) were quantified in the fruits of 22 cultivars/origins of three species of the Chinese hawthorn (Crataegus spp.) by HPLC-ESI-MS-SIR. Hyperoside (0.1-0.8 mg/g dry mass [DM]), isoquercitrin (0.1-0.3 mg/g DM), chlorogenic acid (0.2-1.6 mg/g DM), epicatechin (0.9-11.7 mg/g DM), PA B2 (0.7-12.4 mg/g DM), PA dimer II (0.1-1.5 mg/g DM), PA trimer I (0.1-2.7 mg/g DM), PA trimer II (0.7-6.9 mg/g DM), PA trimer III (0.01-1.2 mg/g DM) and a PA dimer-hexoside (trace-1.1 mg/g DM) were detected in all the samples. Ideain (0.0-0.7 mg/g DM) was found in all the samples except Crataegus scabrifolia. Significant correlations between the contents of individual PA aglycons were observed (r > 0.9, P < 0.01). A strong correlation between flavonols was also shown (r = 0.71, P < 0.01). Fruits of Crataegus pinnatifida var. major had higher contents of PAs but lower contents of flavonols compared with Crataegus brettschneideri. The fruits of C. scabrifolia contained the highest level of PA dimer-hexoside, which was present in trace amounts in the fruits of C. pinnatifida. © 2011 Elsevier Ltd. All rights reserved.


Dong X.,Hong Kong University of Science and Technology | Dong X.,Jinan University | Yang W.,Hong Kong University of Science and Technology | Hu W.,Hong Kong University of Science and Technology | Sun J.,Hong Kong University of Science and Technology
Angewandte Chemie - International Edition | Year: 2015

The asymmetric fluorination of azolium enolates that are generated from readily available simple aliphatic aldehydes or α-chloro aldehydes and N-heterocyclic carbenes (NHCs) is described. The process significantly expands the synthetic utility of NHC-catalyzed fluorination and provides facile access to a wide range of α-fluoro esters, amides, and thioesters with excellent enantioselectivity. Pyrazole was identified as an excellent acyl transfer reagent for catalytic amide formation. © 2015 Wiley-VCH Verlag GmbH & Co. KGaA.


Zhu X.,Hong Kong University of Science and Technology | Zhu X.,Jinan University | Xiang Y.,Hong Kong University of Science and Technology
Philosophical Magazine | Year: 2010

In this paper, we present a continuum model for dislocation dynamics in a slip plane, which accurately incorporates both the long-range interaction and the local line tension effect of dislocations. Unlike the continuum models in the literature using dislocation densities, we use the disregistry across the slip plane to represent the continuous distribution of dislocations in the slip plane, which has the advantage of including the orientation dependence of dislocations in a very simple way. The continuum dislocation dynamics model is validated by linear instability analysis of a uniform dislocation array to small perturbations and comparisons of the results with those of the discrete dislocation dynamics model. Numerical examples for the evolution of distributions of dislocations and plastic slips in a slip plane are presented. © 2010 Taylor & Francis.


Liang X.,Jinan University | Nie X.,Jinan University | Ying G.,CAS Guangzhou Institute of Geochemistry | An T.,CAS Guangzhou Institute of Geochemistry | Li K.,Chinese Academy of Fishery Sciences
Chemosphere | Year: 2013

The toxic effects of triclosan (TCS) on the swordtail fish (Xiphophorus helleri) were assessed based on various biomarkers including enzymatic activities of ethoxyresorufin O-deethylase (EROD), erythromycin N-demethylase (ERND) and glutathione-s-transferase (GST) and mRNA expression levels of CYP1A, CYP3A, glutathione S-transferase (GST) and P-glycoprotein (P-gp). The acute toxicity test showed the LC50 value of 1.47mgL-1 for TCS. The mRNA expressions of CYP1A, CYP3A, GST and P-gp showed dose-effect relationships in female swordtail fish when exposed to TCS, These mRNA expression levels were found more sensitive to TCS exposure than the enzymatic activities of EROD, ERND and GST do. In addition, the male fish displayed higher gene expression levels and more dramatic changes in enzyme activities than the females did. Our data further demonstrated that TCS was a typical inducer to Phase I and Phase II metabolism enzymes and genes, suggesting it is a potential ecotoxicological risk to aquatic ecosystems. © 2012 Elsevier Ltd.


Chen D.,Hong Kong University of Science and Technology | Chen D.,Jinan University | Chen C.,Hong Kong University of Science and Technology | Baiyee Z.M.,Hong Kong University of Science and Technology | And 3 more authors.
Chemical Reviews | Year: 2015

The recent advances in the development of nonstoichiometric oxides, ranging from simple oxide, perovskite, layered perovskite, and pyrochlore, for oxygen reduction Reaction (ORR) and oxygen evolution reaction (OER) in metal-air batteries (MABs) and low-temperature fuel cells (LTFCs) are reviewed. These catalysts are characterized to be low cost and earth-abundant, as well as possess relatively high activity and stability under operation conditions. It is expected that these catalysts will be essential to the future development of multiple technologies. It is expected that the development of nonstoichiometric oxides, with the mutual development of system components, will lead to highly stable and efficient MABs and LTFCs in practical applications in the near future. The electrochemical strain microscopy technique may provide a direct visualization of the ORR/OER activation process on the scale of several nanometers and provide nanoscale understanding into local kinetics. An efficient approach to discover new materials with high intrinsic activities is to tune the electronic structure of existing materials.


Yuan X.,Jinan University | Yuan D.,Jinan University | Zeng F.,Jinan University | Zou W.,Jinan University | And 3 more authors.
Applied Catalysis B: Environmental | Year: 2013

A graphitic mesoporous carbon (GMC) had been successfully synthesized using Ni-Fe layered double hydroxide (LDH) as both template and catalyst under a relatively low pyrolysis temperature. The techniques of X-ray diffraction, transmission electron microscopy, Raman spectrum and N2 adsorption/desorption were used to characterize the physico-chemical properties of the as-prepared GMC. Meanwhile, the voltammetric behaviors of hydroquinone (HQ) and catechol (CC) were studied at the GMC modified glassy carbon electrode (GMC/GCE). The separation of the oxidation and reduction peak (ΔEp) for HQ and CC were decreased from 369 to 42mV and from 365 to 52mV, respectively, and the anodic peak currents for the oxidation of both HQ and CC were also remarkably increased at the GMC/GCE. Furthermore, at the GMC/GCE, the two components could be entirely separated with a large oxidation peak potential separation between HQ and CC. Under the optimized condition, the peak currents of HQ and CC increased linearly with increasing HQ and CC contents. The detection limit for HQ and CC was 3.7×10-7 and 3.1×10-7molL-1, respectively. © 2012 Elsevier B.V.


Li C.,Jinan University | Liu Y.,Jinan University | Zeng Q.-Y.,Sun Yat Sen University | Ao N.-J.,Jinan University
Materials Letters | Year: 2013

Natural rubber was commonly endowed with antimicrobial activity by adding low molecular weight biocidal agents. Herein we report a new antimicrobial rubber prepared by chemical modification. Quaternary phosphonium salt (QPs) groups were covalently immobilized onto the molecular chains of epoxidized natural rubber (ENR) using halogenated acid as an intermediary. The structures of modified rubbers were confirmed while the antimicrobial activity of QP modified ENR was investigated. Results show that the ring-opening ENR and the QP modified ENR are successfully prepared. The proliferation of microorganisms tested can be effectively inhibited by using QP modified ENR. Meanwhile, the modified ENR illustrates better antimicrobial activity toward Gram-negative E. coli than Gram-positive S. aureus. This method can provide potential applications in preparing permanent antimicrobial rubbers. © 2012 Elsevier B.V.


Xie S.,Sun Yat Sen University | Lu X.,Sun Yat Sen University | Zhai T.,Sun Yat Sen University | Gan J.,Sun Yat Sen University | And 5 more authors.
Langmuir | Year: 2012

We report the synthesis of Zn xCd 1-xS@ZnO nanorod arrays via a facile two-step process and the implementation of these core-shell nanorods as an environmental friendly and recyclable photocatalyst for methyl orange degradation. The band gap of Zn xCd 1-xS@ZnO core-shell nanorods can be readily tunable by adjusting the ratio of Zn/Cd during the synthesis. These Zn xCd 1-xS@ZnO core-shell nanorods exhibit a high photocatalytic activity and good stability in the degradation of the methyl orange. Moreover, these films grown on FTO substrates make the collection and recycle of the photocatalyst easier. These findings may open new opportunities for the design of effective, stable, and easy-recyclable photocatalytic materials. © 2012 American Chemical Society.


Zhang Y.,Qingzhou Hospital of Traditional Chinese Medicine | Tang S.,Jinan University | Chen G.,Qingzhou Hospital of Traditional Chinese Medicine | Liu Y.,Qingzhou Hospital of Traditional Chinese Medicine
Complementary Therapies in Medicine | Year: 2015

Objective: To determine the effect of Chinese massage combined with core stability exercises on nonspecific low back pain. Methods: In the prospective study, ninety-two participants with nonspecific low back pain were divided into experimental and control group at random, and 46 in each. The experimental group were treated using Chinese massage combined with core stability exercises, while the control group were treated using Chinese massage alone. The two groups were evaluated using visual analog scale and Oswestry disability index at baseline, immediately after two and eight weeks. In addition, the recurrence rate of nonspecific low back pain was evaluated one year after the last intervention. Results: Two weeks after treatment, both VAS and ODI scores decreased significantly in two groups (p<. 0.05), when compared with the values before treatment, but no difference between the two groups (p>. 0.05). Eight weeks later, the VAS and ODI scores decreased significantly in both groups (p<. 0.05); at the same time, both VAS and ODI scores were significantly lower (p<. 0.05) in the experimental group than those in the control group. At the final follow-up, five cases recurred in the experimental group and nineteen cases in the control group, the control group has a significantly higher recurrence rate (p<. 0.05). Conclusion: Core stability exercises can improve the therapeutic effect of Chinese massage in treating nonspecific low back pain. © 2014 Elsevier Ltd.


Zeng Y.-K.,Sun Yat Sen University | Zeng Y.-K.,Guangzhou Medical College | Yang Z.-L.,Sun Yat Sen University | Peng J.-S.,Sun Yat Sen University | And 2 more authors.
Annals of Surgery | Year: 2012

Objective: To evaluate the safety and efficacy of laparoscopy-assisted distal gastrectomy (LADG) in patients with early gastric cancer (EGC) to determine whether LADG is an acceptable alternative to open distal gastrectomy (ODG). Background: LADG combined with less than D2 or D2 lymphadenectomy for EGC is still a controversial surgical intervention for its uncertain oncological safety and economic benefit. We conducted this systematic review and meta-analysis that included randomized control trials (RCTs) and non-RCTs of LADG versus ODG to evaluate whether the safety and efficacy of LADG in patients with EGC are equivalent to those of ODG. Methods: A comprehensive search of PubMed, EMBASE, Cochrane Library, and China Knowledge Resource Integrated Database was performed. Eligible trials published between January 1, 1994, and December 31, 2010, were included in the study. Data synthesis and statistical analysis were carried out by RevMan 5.0 software. The quality of evidence was assessed by GRADEpro 3.2.2. Results: Twenty-two studies with 3411 participants were included in this study. The mean number of lymph nodes retrieved in LADG was close to that retrieved in ODG (in the less than D2 resection: weighted mean difference [WMD] =-1.79; 95% confidence interval [95% CI],-5.78 to 2.19; P = 0.38; heterogeneity: P < 0.00001, I = 98%; and in the D2 resection: WMD =-1.53; 95% CI,-3.56 to 0.51; P = 0.14; heterogeneity: P = 0.23, I = 26%). The overall postoperative morbidity was significantly less in LADG than in ODG (relative risk = 0.58; 95% CI, 0.46-0.74; P < 0.00001; heterogeneity: P = 0.94, I = 0%). LADG reduced the intraoperative blood loss, postoperative analgesic consumption, and hospital duration, without increasing the total hospitalization costs and cancer recurrence rate. The long-term survival rate of patients undergoing LADG was similar to that of patients undergoing ODG. However, LADG was still a technically dependent and time-consuming procedure. Conversion rate of LADG was 0% to 2.94%. The reported reasons for conversion were bleeding, adhesion, and safety resection margin requirement. Limitations: There were potential biases and significant heterogeneity in some clinical outcome measures in this study. Methodologically high-quality controlled clinical trials were sparse for this new surgical intervention. According to The Grading of Recommendations Assessment, Development and Evaluation approach, when assessing the safety and efficacy of LADG by comparing with those of ODG with the defined clinical outcomes in patients with EGC, the quality of the currently available clinical evidence was very low. Conclusions: LADG may be a technically feasible alternative for EGC when it is performed in experienced surgical centers in which patients undergoing LADG may benefit from the faster postoperative recovery. However, the currently available evidence cannot exclude the potential clinical benefits or harms, especially in the node-positive cases. Methodologically high-quality comparative studies are needed for further evaluation. © 2012 by Lippincott Williams & Wilkins.


Wang T.,Jinan University | Feng Z.-Q.,Sun Yat Sen University | Leach M.K.,University of Michigan | Wu J.,Dow Chemical Company | Jiang Q.,Sun Yat Sen University
Journal of Materials Chemistry B | Year: 2013

Advanced scaffold materials are required for liver tissue engineering, to improve primary hepatocyte activity and hepatic function in vitro. The nanotopography of the scaffold material plays an important role in the regulation of cell growth and function. Therefore, in the current study, we developed a novel scaffold composed of type-I collagen coated nanoporous poly(L-lactic acid) (PLLA) fibers (nPFs) to provide a nanotopography with a combination of fibrous and porous features for the culture of primary hepatocytes. The interaction between the nanotopography and the hepatocytes was described by testing cell morphology, retention, activity and hepatic function over a 15 day culture period. Primary hepatocytes cultured on the nPFs formed large-area stable immobilized monolayers after 3 days of culture, and displayed excellent cell bioactivity with higher levels of liver-specific function maintenance, in terms of albumin secretion, urea synthesis, and CYP1A and UGT enzymatic activity, than those cultured on type-I collagen coated non-porous PLLA fibers (Fs). These results indicate that the combined fibrous and porous nanotopography of nPFs has a superior promoting effect on primary hepatocyte culture compared to the non-porous fibrous nanotopography of Fs. The nPFs may be a suitable material for liver tissue engineering research and potential therapeutic applications, such as in bioartificial liver devices, and as a substrate for primary hepatocyte culture. © 2013 The Royal Society of Chemistry.


Ge R.,Sun Yat Sen University | Sun X.,Jinan University | Wang D.,Sun Yat Sen University | Zhou Q.,Sun Yat Sen University | Sun H.,University of Hong Kong
Biochimica et Biophysica Acta - Molecular Cell Research | Year: 2011

Helicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. © 2011 Elsevier B.V.


Weilong W.,Sun Yat Sen University | Xiaobo F.,Jinan University
Journal of Nanomaterials | Year: 2013

Fe/Mn mixed metal oxides were synthesized facilely by a grinding method and were characterized by TEM, XRD, XPS, and BET. The characterization results revealed that mixed metal oxides were mainly composed of not highly crystallized Fe2O3 and Mn3O4 nanoparticles with a diameter about 3-5 nm. The specific BET surface areas of the composite were affected by the amounts of KCl diluent in the preparation process and about 268 m2/g of the composite can be achieved. Compared with metal oxide adsorbents existent, the composites showed good adsorption capacity, stability, and regeneration activity for Cr(VI) removal. The enhanced adsorption capacity was speculated to be ascribed to the synergistic effect of the mixed metal oxides. By monitoring the valence change in the adsorption process using XPS characterization, the mechanism for Cr(VI) removal on the composites was found to be a combination of electrostatic attraction and ion exchange. The above results demonstrated that the synthesized metal oxides nanocomposite is of great potential for Cr(VI) removal in the fields of remediation of environmental problems. © 2013 Wang Weilong and Fu Xiaobo.


He G.-N.,Sun Yat Sen University | Huang B.,Jinan University | Shen H.,Sun Yat Sen University
Journal of Crystal Growth | Year: 2010

Vertically aligned arrays of ZnO nanorod were synthesized on the Au/SiO2/Si(1 0 0) substrate by a simple aqueous solution growth process, without pre-prepared ZnO seed layer. For comparison, glass and SiO 2/Si were also used as substrates, and the results show that the Au layer plays a decisive role in orienting the growth of the ZnO nanorod. The effects of other growth parameters, including Zn2 concentration and growth time, on morphology, density, and orientation of the ZnO nanostructure were also studied and with longer reaction time, a new structure namely ZnO nanotip was obtained. Moreover, the growth mechanism of ZnO nanorod arrays grown on the Au/SiO2/Si substrate was proposed. © 2010 Elsevier B.V. All rights reserved.


Ge R.,Sun Yat Sen University | Sun X.,Jinan University
Metallomics | Year: 2014

Gram-positive Streptococcus species are responsible for millions of cases of meningitis, bacterial pneumonia, endocarditis, erysipelas and necrotizing fasciitis. Iron is essential for the growth and survival of Streptococcus in the host environment. Streptococcus species have developed various mechanisms to uptake iron from an environment with limited available iron. Streptococcus can directly extract iron from host iron-containing proteins such as ferritin, transferrin, lactoferrin and hemoproteins, or indirectly by relying on the employment of specialized secreted hemophores (heme chelators) and small siderophore molecules (high affinity ferric chelators). This review presents the most recent discoveries in the iron acquisition system of Streptococcus species-the transporters as well as the regulators. This journal is © the Partner Organisations 2014.


Zhang L.,Jinan University | Du L.,Jinan University | Yu X.,Jinan University | Tan S.,Jinan University | And 4 more authors.
ACS Applied Materials and Interfaces | Year: 2014

Electron-hole recombination is one of the major factors limiting the efficiency of ZnO-based photocatalysts. In this work, a 2-fold enhancement strategy was employed to suppress electron-hole recombination and boost photocatalytic efficiency. First, significantly enhanced photocatalytic activity of ZnO by introducing graphene oxide (GO) was systematically investigated. Hybrid photocatalysts with different weight ratios of ZnO to GO (from 0.95:0.05 to 0.70:0.30) were synthesized and characterized. The results indicated that when the proportion ratio of ZnO to GO reached 0.85:0.15, the as-synthesized ZnO-GO nanocomposite exhibited the maximum photocatalytic efficiency on methylene blue with an apparent rate constant κapp almost 10 times faster than that of pure ZnO under UV illumination. GO was suggested to enhance the photocatalytic activity of ZnO because of its great capability in dye adsorption and charge separation. Second, Pd nanoparticles were introduced to decorate ZnO-GO to produce generally better photocatalyst ZnO-GO-Pd nanocomposites. The junction between Pd and ZnO was believed to also effectively separate the photogenerated charges due to the metal-semiconductor diode effect. These two systems of ZnO-GO and ZnO-GO-Pd nanocomposites are expected to have a broad range of applications in environmental conservation. © 2014 American Chemical Society.


Wang X.,Chinese University of Hong Kong | Xie W.,Jinan University | Chen J.,Sun Yat Sen University | Xu J.-B.,Chinese University of Hong Kong
ACS Applied Materials and Interfaces | Year: 2014

p-n junction is a fundamental building block in modern electronic circuits. We report graphene p-n junctions formed by a one-step thickness-dependent surface treatment of mono-/bilayer graphene steps. The junction electronic properties are systemically studied by means of Kelvin probe force microscopy (KPFM) and transport measurements. Because of the dissimilar modifications to graphene electronic properties, the junctions behave distinctly, i.e., two-component resistance-like for organic charge transfer doping and Shottky-junction-like for covalent doping. By exploring the spatially potential distribution, we clarify the potential profiles as well as the transport attributes across the graphene p-n junction interface under lateral bias and electrical gating. Our results not only unveil the detailed properties of graphene p-n junction interface, but also gain an insight into its practical applications in nanoelectronics. © 2013 American Chemical Society.


Lu Y.-Y.,South China Normal University | Chen T.-S.,Sun Yat Sen University | Wang X.-P.,Jinan University | Li L.,Sun Yat Sen University
Journal of Biomedical Optics | Year: 2010

Dihydroartemisinin (DHA), a front-line antimalarial herbal compound, has been shown to possess promising anticancer activity with low toxicity. We have previously reported that DHA induced caspase-3-dependent apoptosis in human lung adenocarcinoma cells. However, the cellular target and molecular mechanism of DHAinduced apoptosis is still poorly defined. We use confocal fluorescence microscopy imaging, fluorescence resonance energy transfer, and fluorescence recovery after photobleaching techniques to explore the roles of DHA-elicited reactive oxygen species (ROS) in the DHAinduced Bcl-2 family proteins activation, mitochondrial dysfunction, caspase cascade, and cell death. Cell Counting Kit-8 assay and flow cytometry analysis showed that DHA induced ROS-mediated apoptosis. Confocal imaging analysis in a single living cell and Western blot assay showed that DHA triggered ROS-dependent Bax translocation, mitochondrial membrane depolarization, alteration of mitochondrial morphology, cytochrome c release, caspase-9, caspase-8, and caspase-3 activation, indicating the coexistence of ROS-mediated mitochondrial and death receptor pathway. Collectively, our findings demonstrate for the first time that DHA induces cell apoptosis by triggering ROS-mediated caspase-8/Bid activation and the mitochondrial pathway, which provides some novel insights into the application of DHA as a potential anticancer drug and a new therapeutic strategy by targeting ROS signaling in lung adenocarcinoma therapy in the future. © 2010 Society of Photo Optical Instrumentation Engineers.


Wang H.,Sun Yat Sen University | Wang H.-S.,Sun Yat Sen University | Zhou B.-H.,Sun Yat Sen University | Li C.-L.,Sun Yat Sen University | And 6 more authors.
PLoS ONE | Year: 2013

Chronic inflammation-promoted metastasis has been considered as a major challenge in cancer therapy. Pro-inflammatory cytokine TNFα can induce cancer invasion and metastasis associated with epithelial-mesenchymal transition (EMT). However, the underlying mechanisms are not entirely clear. In this study, we showed that TNFα induces EMT in human HCT116 cells and thereby promotes colorectal cancer (CRC) invasion and metastasis. TNFα-induced EMT was characterized by acquiring mesenchymal spindle-like morphology and increasing the expression of N-cadherin and fibronectin with a concomitant decrease of E-cadherin and Zona occludin-1(ZO-1). TNFα treatment also increased the expression of transcription factor Snail, but not Slug, ZEB1 and Twist. Overexpression of Snail induced a switch from E-cadherin to N-cadherin expression in HCT116 cells, which is a characteristic of EMT. Conversely, knockdown of Snail significantly attenuated TNFα-induced EMT in HCT116 cells, suggesting that Snail plays a crucial role in TNFα-induced EMT. Interestingly, exposure to TNFα rapidly increased Snail protein expression and Snail nuclear localization but not mRNA level upregulation. Finally, we demonstrated that TNFα elevated Snail stability by activating AKT pathway and subsequently repressing GSK-3β activity and decreasing the association of Snail with GSK-3β. Knockdown of GSK-3β further verified our finding. Taken together, these results revealed that AKT/GSK-3β-mediated stabilization of Snail is required for TNFα-induced EMT in CRC cells. Our study provides a better understanding of inflammation-induced CRC metastasis. © 2013 Wang et al.


Liang H.,Guangdong Polytechnic Normal University | Huang J.,Jinan University | Zhao Y.,Sun Yat Sen University
Nonlinear Dynamics | Year: 2014

This paper is devoted to the complete classification of global phase portraits of quasi-homogeneous but non-homogeneous coprime planar polynomial differential systems of degree (Formula present). To prove our result, we firstly study the canonical forms for these systems. Then, the global topological structures of the systems having canonical forms are studied by using the quasi-homogeneous blow-up technique for the finite singularities and the Poincaré-Lyapunov compactification for the infinite singularities. Finally we perform a topological classification for the set of global phase portraits. © 2014, Springer Science+Business Media Dordrecht.


Dong H.-P.,Xiamen University | Dong H.-P.,Jinan University | Wang D.-Z.,Xiamen University | Xie Z.-X.,Xiamen University | And 2 more authors.
Geochimica et Cosmochimica Acta | Year: 2013

Dissolved organic matter (DOM) is an important reservoir of carbon and energy in the marine environment and plays a key role in regulating the global carbon cycle. This study characterized proteins of high-molecular-weight DOM (size between 5. kDa and <0.2. μm, HMW-DOM) collected from the surface seawaters in the South China Sea using a shotgun proteomic approach in combination with the global ocean sampling combined assembly protein database. A total of 367 protein groups matched by 993 unique peptides from 1991 spectra were identified from four surface HMW-DOM samples. Proteins with unknown taxonomic classification and function dominated the dissolved protein pool (43-53%) while the remaining proteins presented close similarity in biological origin among the four sampling sites. Rhodospirillaceae, Prochlorococcus, SAR11 clade and viruses were the major contributors to dissolved proteins in the HMW-DOM from surface seawaters while very few proteins were from the eukaryotic phytoplankton and no archaeal proteins were detected. Transporters with substrate specificities for nitrogen- and carbon-containing compounds (1.5% of the total spectra for each) were highly detected while no phosphate transporters were found, suggesting that carbon and nitrogen might be more limiting than phosphorus in the surface seawater. Viral proteins were assigned into three families: Myoviridae, Podoviridae and Siphoviridae, and the Myoviridae proteins were the most abundant. Among them, structure proteins were the most abundant viral proteins. This study indicated that the dissolved proteins of HMW-DOM presented compositional and biologically original homogeneity in the surface seawaters of the South China Sea, and bacteria and viruses dominated the dissolved protein pool. © 2013 Elsevier Ltd.


Pan H.-W.,Jinan University | Cui Y.-H.,Guangzhou University | Zeng J.-W.,Sun Yat Sen University
Investigative Ophthalmology and Visual Science | Year: 2014

PURPOSE. To investigate the role of angiotensin II (Ang II) in the regulation of corneal myofibroblast apoptosis and the possible signaling pathway. METHODS. Rabbit corneal myofibroblasts were cultured in vitro and the cell phenotype was identified by expression of a-smooth muscle actin (a-SMA) and formation of F-actin. The expression of Ang II type I receptor (AT1R) in keratocytes and corneal myofibroblasts were detected by immunofluorescence staining and Western blot. The effect of Ang II on corneal myofibroblast apoptosis induced by serum starvation and TNFa plus cycloheximide (CHX) was examined by TUNEL, Hoechst 33258 staining, and caspase 3/7 activity assay. The effect of Ang II on nuclear factor-jB (NF-jB)-dependent DNA binding activity and transcriptional activity was studied by electrophoresis mobility shift assay (EMSA) and luciferase reporter assay, respectively. Ang II-induced TGFb1 secretion by corneal myofibroblasts was determined by ELISA. RESULTS. Ang II type I receptor expression was more abundant in corneal myofibroblasts compared with keratocytes. Ang II reduced corneal myofibroblasts apoptotic response to serum starvation or treatment with TNFa plus CHX. This protective effect was attenuated in the presence of AT1R antagonist losartan or NF-jB-specific inhibitor Bay11-7082. Ang II increased NF-jB-dependent DNA-binding activity and transcriptional activity, and also increased TGFb1 production by corneal myofibroblasts. CONCLUSIONS. Ang II induces corneal myofibroblasts resistance to apoptosis via activating NFjB signaling pathway, and thus should be further investigated as a possible target for therapy of corneal fibrosis. © 2014 The Association for Research in Vision and Ophthalmology, Inc.


Chen Y.,Sun Yat Sen University | Qiao L.,Sun Yat Sen University | Yu B.,Sun Yat Sen University | Li G.,Sun Yat Sen University | And 3 more authors.
Chemical Communications | Year: 2013

An AIPE-active iridium(iii) complex was found to possess high specificity for mitochondria, superior photostability, low cytotoxicity, and high resistance to the loss of mitochondrial membrane potential. Thus, this complex can be used for mitochondrial imaging and tracking in living cells. © 2013 The Royal Society of Chemistry.


Tian W.-J.,Shenyang Pharmaceutical University | Yu Y.,Jinan University | Yao X.-J.,Macau University of Science and Technology | Chen H.-F.,Xiamen University | And 4 more authors.
Organic Letters | Year: 2014

Norsampsones A-D (1-4), four new decarbonyl polycyclic polyprenylated acylphloroglucinols, together with a new biogenetically related compound hypersampsone M (5), were isolated from the aerial parts of Hypericum sampsonii. Norsampsones A-D featured an unprecedented carbon skeleton with the loss of C-2 carbonyl in the phloroglucinol ring. All structures were determined by extensive NMR spectroscopic methods, ECD calculation, and single-crystal X-ray diffraction. © 2014 American Chemical Society.


Ma D.,Jinan University | Lin Q.-M.,Jinan University | Zhang L.-M.,Sun Yat Sen University | Liang Y.-Y.,Hangzhou Normal University | Xue W.,Jinan University
Biomaterials | Year: 2014

The co-delivery of drug and gene has become the primary strategy in cancer and other disease therapy. To co-deliver hydrophobic drug and functional gene efficiently into tumor cells, a star-shaped copolymer (PP-PLLD-Arg) with a photochemical internalization effect consisting of a porphyrin (PP) core and arginine-functionalized poly(l-lysine) dendron (PLLD-Arg) arms has been designed, and used to co-deliver docetaxel (DOC) and MMP-9 shRNA plasmid for nasopharyngeal cancer therapy. It was found that PP-PLLD-Arg/MMP-9 nanocomplex showed the photo-enhanced gene transfection efficiency in vitro, and could mediate a significant reduce of MMP-9 protein expression in HNE-1 cells. For co-delivery analysis, the obtained PP-PLLD-Arg/DOC/MMP-9 complexes could induce a more significant apoptosis than DOC or MMP-9 used only, and decreased invasive capacity of HNE-1 cells. Moreover, the star-shaped copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k in the hemolysis and MTT assays, and also showed a good biocompatibility in vivo. Therefore, PP-PLLD-Arg with suited irradiation is a promising non-toxic and photo-inducible effective drug and gene delivery strategy, which should be encouraged in tumor therapy. © 2014 Elsevier Ltd.


Ge R.,Sun Yat Sen University | Sun X.,Jinan University
BioMetals | Year: 2012

Helicobacter pylori infections are closely associated with peptic ulcers, gastric malignancy and iron deficiency anemia. Iron is essential for almost all living organisms and the investigation of iron uptake and trafficking system is thus important to understand the pathological roles of H. pylori. Up to now, the iron trafficking system of H. pylori is not yet fully clear and merits further efforts in this regards. The available information about iron uptake and regulation has been discussed in this concise review, such as FeoB in ferrous transportation, FrpB2 in hemoglobin uptake, HugZ in heme processing, virulence factors (VacA and CagA) in transferrin utilization, Pfr and NapA in iron storage and Fur in iron regulation. The identified iron trafficking system will help us to understand the pathological roles of H. pylori in the various gastric diseases and iron deficiency anemia and stimulates further development of effective anti-bacterial drugs. © Springer Science+Business Media, LLC. 2011.


Fang R.,Sun Yat Sen University | Zhang G.,Sun Yat Sen University | Guo Q.,Sun Yat Sen University | Ning F.,Sun Yat Sen University | And 3 more authors.
Cancer Letters | Year: 2013

Nodal, an important embryonic morphogen, has been reported to function in tumorigenesis. Here we report for the first time that Nodal promotes malignancy by inducing epithelial-mesenchymal transition (EMT) in B16 murine melanoma. These cells displayed increased migration and invasion abilities upon treating with Nodal, accompanying with typical phenotype changes of EMT. In contrast, Nodal knockdown or blocking Nodal signaling using a specific antagonist SB431542 repressed the EMT phenotype as well as reduced cell motility and invasiveness. Treatment with Nodal also induced expression of transcription factor Snail. Snail knockdown abolished the Nodal-induced EMT in B16 cells. We further show that Snail expression is mediated by the Nodal-regulated AKT/GSK-3β signaling. Taken together, these results revealed that Nodal promotes the aggressive phenotype of B16 murine melanoma cells by inducing EMT via up-regulation of Snail. This study provides a better understanding of Nodal function in melanoma, and suggests a potential novel target for clinical therapeutic research. © 2013 Elsevier Ireland Ltd.


Zhang D.-M.,Jinan University | Liu J.-S.,Jinan University | Deng L.-J.,Jinan University | Chen M.-F.,Jinan University | And 7 more authors.
Carcinogenesis | Year: 2013

Hepatocellular carcinoma (HCC) is a deadly form of cancer without effective chemotherapy so far. Currently, only sorafenib, a multitargeted tyrosine kinase inhibitor, slightly improves survival in HCC patients. In searching for natural anti-HCC components from toad venom, which is frequently used in the treatment of liver cancer in traditional Chinese medicine, we discovered that arenobufagin, a bufadienolide from toad venom, had potent antineoplastic activity against HCC HepG2 cells as well as corresponding multidrug-resistant HepG2/ADM cells. We found that arenobufagin induced mitochondria-mediated apoptosis in HCC cells, with decreasing mitochondrial potential, as well as increasing Bax/Bcl-2 expression ratio, Bax translocation from cytosol to mitochondria. Arenobufagin also induced autophagy in HepG2/ ADM cells. Autophagy-specific inhibitors (3-methyladenine, chloroquine and bafilomycin A1) or Beclin1 and Atg 5 small interfering RNAs (siRNAs) enhanced arenobufagin-induced apoptosis, indicating that arenobufagin-mediated autophagy may protect HepG2/ADM cells from undergoing apoptotic cell death. In addition, we observed the inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway by arenobufagin. Interestingly, inhibition of mTOR by rapamycin or siRNA duplexes augmented arenobufagin-induced apoptosis and autophagy. Finally, arenobufagin inhibited the growth of HepG2/ADM xenograft tumors, which were associated with poly (ADP-ribose) polymerase cleavage, light chain 3-II activation and mTOR inhibition. In summary, we first demonstrated the antineoplastic effect of arenobufagin on HCC cells both in vitro and in vivo. We elucidated the underlying antineoplastic mechanisms of arenobufagin that involve cross talk between apoptosis and autophagy via inhibition of the PI3K/Akt/mTOR pathway. This study may provide a rationale for future clinical application using arenobufagin as a chemotherapeutic agent for HCC. © The Author 2013. Published by Oxford University Press.All rights reserved.


Xiao X.,Huazhong University of Science and Technology | Li T.,Huazhong University of Science and Technology | Yang P.,Jinan University | Gao Y.,Huazhong University of Science and Technology | And 14 more authors.
ACS Nano | Year: 2012

All-solid-state flexible supercapacitors based on a carbon/MnO 2 (C/M) core-shell fiber structure were fabricated with high electrochemical performance such as high rate capability with a scan rate up to 20 V s -1, high volume capacitance of 2.5 F cm -3, and an energy density of 2.2 × 10 -4 Wh cm -3. By integrating with a triboelectric generator, supercapacitors could be charged and power commercial electronic devices, such as a liquid crystal display or a light-emitting-diode, demonstrating feasibility as an efficient storage component and self-powered micro/nanosystems. © 2012 American Chemical Society.


Lin Z.,Jinan University | Guan Z.,Jinan University | Zhang Z.,Sun Yat Sen University | Mai K.,Sun Yat Sen University
Thermochimica Acta | Year: 2012

It has been proved that the effect of amorphous polymer as the second component on the β-nucleating effect of β-nucleated isotactic polypropylene (iPP) blends was less than that of the crystalline polymers. We guess that it is the crystallinity of the second component that affects the β-nucleating effect. In order to prove this hypothesis, the blends of β-nucleated iPP and polyamide 6 (PA6) were prepared by melt compounding in this study. Cooling rate, crystallization temperature, crystallization time, annealing temperature and annealing time were used to control the crystallinity of PA6 phase, and the effect of PA6 crystallinity on the crystallization and melting behavior was studied, in turn its effect on the β-nucleating effect of iPP phase was explored. The results indicated that β crystal content markedly decreased with the increase of PA6 crystallinity, which proved that PA6 crystallinity controls the α-nucleating effect and suppresses the β-nucleating effect of the β-nucleating agent in the β-nucleated iPP blends, leading to a significant decrease in β crystal content. Therefore, decreasing the crystallinity of the second component in the β-nucleated iPP blends is a feasible method to weaken α-nucleating effect of the second component and an effective way to obtain β-nucleated iPP blend with high β crystal content. © 2012 Elsevier B.V.


Rui Z.,Sun Yat Sen University | Huang Y.,Sun Yat Sen University | Zheng Y.,Sun Yat Sen University | Ji H.,Sun Yat Sen University | Yu X.,Jinan University
Journal of Molecular Catalysis A: Chemical | Year: 2013

Anatase and rutile have the same chemical composition as TiO2 but different crystalline structures and surface structures, which draws effect on the properties of their supported catalysts. Additionally, titania mixture, e.g., Degussa P25, which consists of both anatase and rutile, presents different physicochemical properties with anatase and rutile. In this work, P25-, anatase- or rutile-supported copper oxide catalysts (signed as CuO/P25, CuO/A and CuO/R) were prepared and characterized to study the titania support effect on the physicochemical properties and catalytic properties for the trace CH 4 combustion. It is found that the activity of CuO/R is better than the corresponding CuO/A sample when their pore size and specific surface area are comparable, which is attributed to the different chemical states of the copper oxide caused by the different TiO2 support polymorphs. The loading of CuO and subsequent calcination promotes more significant sintering and anatase to rutile transform for CuO/P25 than those for CuO/A. Thus, CuO/R shows better stability than CuO/P25. © 2013 Elsevier B.V.


Yin H.-Q.,Sun Yat Sen University | Mai D.-S.,Jinan University | Gan F.,Sun Yat Sen University | Chen X.-J.,Jinan University
RSC Advances | Year: 2014

We developed an easy one-step method for the synthesis of linear and cyclic arginine-glycine-aspartate (RGD) conjugated gold nanoparticles (GNPs). Compared with the previously reported multistep process, our method is simple, time-efficient, and cost-effective. Interestingly, the RGD peptides can function as reducing, protecting and tumour targeting agents and the prepared GNPs possess good stability and good biocompatibility. The uptake of RGD-GNPs by human tumour cells was assessed by dark-field light scattering imaging and transmission electron microscopy (TEM). Both types of RGD-GNPs can specifically target the melanoma A375 cells better than the breast cancer MCF-7 cells, which express the integrin αvβ3 at low level. We also compared the cellular uptake ability between the linear and cyclic RGD conjugated GNPs quantitatively. In order to validate the cellular uptake mechanism, the competitive inhibition assay was performed. Various important results indicated that the activity of the RGD peptides to specifically target tumour cells was not influenced noticeably after conjugation with GNPs. © 2014 The Royal Society of Chemistry.


Zhao S.,Jinan University | Ma X.,Sun Yat Sen University
IEEE Photonics Technology Letters | Year: 2016

Visible light communication (VLC) systems are expected to support a variety of applications, such as common-information broadcasting, real-time multimedia streaming, and large file downloading. Typically, these applications have different delay requirements. Hence, it is desirable to design high-performance coding scheme capable of supporting a wide range of delays but with acceptable hardware complexity. To achieve this, we propose a delay-tunable coding scheme for VLC systems based on block Markov superposition transmission of short non-binary low-density parity-check (NBLDPC) codes. The proposed coding scheme includes the following advantages: 1) it is easily configurable to fulfill different delay requirements while keeping the code rate constant; 2) it requires essentially the same hardware modules to implement the encoder/decoder as the involved short NBLDPC code; and 3) it can have a larger coding gain if a longer delay is tolerated. Numerical results are presented to show the expected tradeoff between delay and energy in a VLC system. © 1989-2012 IEEE.


Lin X.,Central South University | Jiang C.,Jinan University | Luo Z.,Central South University | Qu S.,Hunan Normal University
BMC Nephrology | Year: 2013

Background: The protective effect of Erythropoietin (EPO) analogue rHuEPO on acute renal injury induced by exhaustive exercise had been reported. The purpose of this study is to probe into the protective effect of EPO on chronic renal injury induced by repeated exhaustive exercise for four weeks. Methods. Eighty adult male Sprague-Dawley rats were used in this experiment. The animals were randomly allocated to one of four groups: control (C), exhaustive exercise test (ET), ET plus EPO pre-treatement (ET+EPO) and ET+EPO plus LY294002 pretreatment (ET+EPO+LY). Results: Compared with the rats in control group, there was considerable damage in kidney cells in rats of ET group as revealed by histological and ultrastructural examinations. However, treatment with EPO during the training, the exhaustive running distance was significant increased (P < 0.01), and the pathological changes of kidney cell were much less compared with those of rats without EPO intervention. When LY294002, a specific inhibitor of phospholipids phthalocyanine inositol 3-kinase, was added to the EPO treated rats, the injury changes of renal cell were becoming more pronounced. Conclusions: The protective effect of EPO on chronic renal injury induced by repeated exhaustive exercise was demonstrated in the present study. We proposed that the effect could be due to inhibiting the cell apoptosis and blocking the formation of interstitial fibrosis via activation of the PI3K/Akt pathway, thus plays role in the endogenous protection of the kidney injury. © 2013 Lin et al.; licensee BioMed Central Ltd.


Ma D.,Sun Yat Sen University | Ma D.,Jinan University | Zhang L.-M.,Sun Yat Sen University
Materials Science and Engineering C | Year: 2013

The supramolecular hydrogel self-assembled from α-cyclodextrin (α-CD) and an amphiphilic triblock copolymer was used for the first time as a biosensing platform by the in-situ incorporation of horseradish peroxidase and polyaniline (PANI) nanoparticles. It was found that the used triblock copolymer could disperse well PANI nanoparticles in aqueous system and then interact with α-CD in the presence of horseradish peroxidase for the formation of supramolecular hydrogel composite. The content of PANI nanoparticles was found to affect the gelation time and gel strength. The circular dichroism analyses showed that the entrapped horseradish peroxidase could retain its native conformation. By electrochemical experiments, the incorporated PANI nanoparticles were confirmed to improve the current response and enzymatic activity, and the fabricated biosensor was found to provide a fast amperometric response to hydrogen peroxide. © 2013 Elsevier B.V.


Xue Y.-Q.,Jinan University | Di J.-M.,Sun Yat Sen University | Luo Y.,Sun Yat Sen University | Cheng K.-J.,Lishui Institute of Agricultural science | And 2 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2014

Resveratrol (3,4′,5-trihydroxystilbene) is a naturally derived phytoalexin stilbene isolated from grapes and other plants, playing an important role in human health and is well known for its extensive bioactivities, such as antioxidation, anti-inflammatory, anticancer. In addition to resveratrol, scientists also pay attention to resveratrol oligomers, derivatives of resveratrol, which are characterized by the polymerization of two to eight, or even more resveratrol units, and are the largest group of oligomeric stilbenes. Resveratrol oligomers have multiple beneficial properties, of which some are superior in activity, stability, and selectivity compared with resveratrol. The complicated structures and diverse biological activities are of significant interest for drug research and development and may provide promising prospects as cancer preventive and therapeutical agents. This review presents an overview on preventive or anticancer properties of resveratrol oligomers. © 2014 You-Qiu Xue et al.


Sun L.,University of Jinan | Wang S.,Harvard University | Hu C.,Jinan University | Zhang X.,University of Jinan
Experimental Cell Research | Year: 2011

Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell proliferation and apoptosis, it is still unclear how apoptosis is regulated. The aim of this study is to analyze the relationship among apoptosis, phosphatidylinositol 3-kinase (PI3K)/Akt and nuclear factor κB (NF-κB) in FPC knockdown kidney cells. We show that PKHD1-silenced HEK293 cells demonstrate a higher PI3K/Akt activity. Selective inhibition of PI3K/Akt using LY294002 or wortmannin in these cells increases serum starvation-induced HEK293 cell apoptosis with a concomitant decrease in cell proliferation and higher caspase-3 activity. PI3K/Akt inhibition also leads to increased NF-κB activity in these cells. We conclude that the PI3K/Akt pathway is involved in apoptotic function in PKHD1-silenced cells, and PI3K/Akt inhibition correlates with upregulation of NF-κB activity. These observations provide a potential platform for determining FPC function and therapeutic investigation of ARPKD. © 2011 Elsevier Inc.


Liu J.,Jinan University | Soininen J.,University of Helsinki | Han B.-P.,Jinan University | Declerck S.A.J.,Netherlands Institute of Ecology
Journal of Biogeography | Year: 2013

Aim: Dendritic ecological networks (DENs), such as river systems, combine features that challenge the traditional conceptual views and empirical approaches applied to metacommunities. As a result of their dendritic branching geometry and stream flow directionality, they are strongly hierarchical and asymmetrical. We analysed the metacommunity structure of benthic diatoms in a large-scale river system with the aim of evaluating the importance of potential causal influences. Furthermore, we hypothesized that metacommunities of diatoms that are strongly attached to their substrata show a different spatial structure than metacommunities of other, more weakly attached diatoms. Location: The study was carried out in the Dong River, a 32,275 km2 subtropical river network located in southern China. Methods: We surveyed benthic diatom communities during three seasons (dry, intermediate and wet). Using partial redundancy analysis, we partitioned community variation among environmental models and different spatial eigenfunction models to evaluate the influence of alternative dispersal pathways (overland versus water course dispersal), stream directionality, man-made dams and diatom functional traits on diatom metacommunity structure. Results: Models based on hydrological connections and water directionality represent spatial patterns better than overland distances, suggesting that the dynamics of benthic diatom metacommunities are mainly confined to the river network and influenced by the prevailing water flow. We found significant effects of man-made dams on the spatial structure of important limnological variables and diatom metacommunity structure. The metacommunity of strongly attached diatoms also showed a weaker signature of flow directionality than that of other growth forms, especially in seasons with high water levels. Main conclusions: We conclude that the consideration of among-site connectivity, flow directionality and species traits is key to a better understanding of the spatial ecology of passively dispersing microbial organisms in river systems. © 2013 John Wiley & Sons Ltd.


Yan J.,Jinan University | Meng H.,Jinan University | Xie F.,Sun Yat Sen University | Yuan X.,Jinan University | And 4 more authors.
Journal of Power Sources | Year: 2014

Nitrogen-doped hollow mesoporous carbon spheres has been synthesized from mesoporous silica spheres using glycine as carbon and nitrogen precursor. The wall of the spheres is composed by broken graphene. The metal free nitrogen-doped hollow mesoporous carbon spheres are proven to be active electrocatalyst for the oxygen reduction reaction in alkaline solution. A unique advantage of the nitrogen-doped hollow mesoporous carbon sphere is its methanol-tolerant property because of the absence of active metal. The catalytic activity is ascribed to the pyridinic-nitrogen formed during pyrolysis and the graphene-like structure. To the best of our knowledge this is the first report on the nitrogen-doped hollow mesoporous carbon sphere as a metal-free electrocatalyst for the oxygen reduction reaction which is an important reaction in fuel cell. The prepared mesoporous carbon material can also be used as catalyst support and find application both in the anode and cathode of fuel cell. © 2013 Published by Elsevier B.V.


Ge R.,Sun Yat Sen University | Sun X.,Jinan University | Xiao C.,Jinan University | Yin X.,Jinan University | And 3 more authors.
Proteomics | Year: 2011

Increasing evidence shows that protein phosphorylation on serine (Ser), threonine (Thr) and tyrosine (Tyr) residues is a major regulatory post-translational modification in the bacteria. To reveal the phosphorylation state in the Gram-negative pathogenic bacterium Helicobacter pylori, we carried out a global and site-specific phosphoproteomic analysis based on TiO2-phosphopeptide enrichment and high-accuracy LC-MS/MS determination. Eighty-two phosphopeptides from 67 proteins were identified with 126 phosphorylation sites, among which 79 class I sites were determined to have a distribution of 42.8:38.7:18.5% for the Ser/Thr/Tyr phosphorylation, respectively. The H. pylori phosphoproteome is characterized by comparably big size, high ratio of Tyr phosphorylation, high abundance of multiple phosphorylation sites in individual phosphopeptides and over-representation of membrane proteins. An interaction network covering 28 phosphoproteins was constructed with a total of 163 proteins centering on the major H. pylori virulence factor VacA, indicating that protein phosphorylation in H. pylori may be delicately controlled to regulate many aspects of the metabolic pathways and bacterial virulence. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Lin Z.,Jinan University | Zhang Z.,Sun Yat Sen University | Mai K.,Sun Yat Sen University
Journal of Applied Polymer Science | Year: 2012

In this article, a novel eggshell/β-PP bio-composite was prepared and evaluated. First the waste chicken eggshell (ES) was modified by a stingy amount of pimelic acid (PA) through solution method, and then compounded with PP through melt blending method. The crystalline form, mechanical properties, and morphology of the ES/β-PP composites were studied. The results showed that ES modified by PA was a β nucleating agent with high efficiency and selectivity, resulting in the maximum K β value of 0.99 in PP composites. PA modification promoted the dispersion and interfacial bonding of ES in PP. Although the addition of PA-modified ES slightly decreased tensile properties and flexural properties of PP, it increased the impact strength of PP by 228% than that of pure PP. Taking all factors into consideration, the optimal mass ratio of PP/ES/PA composites for reaching optimum mechanical properties should be 100/5/0.000471. Thus, the application of modified ES to prepare ES/β-PP bio-composites represents a promising way to mitigate environmental pollution and to reduce the cost of polyolefin products. Copyright © 2011 Wiley Periodicals, Inc.


Ge R.,Sun Yat Sen University | Sun X.,Jinan University
Medical Hypotheses | Year: 2011

Helicobacter pylori causes such gastric diseases as gastritis, peptic ulcerations, gastric cancer and MALT lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms amyloid-like oligomers in physiologically relevant conditions. Here we proposed the in vivo functions of this protein with relevance to its physical locations. The collective evidence presented here shed some light on the pathologic mechanisms of H. pylori infections, with emphasis on the bacterial colonization in the gastric environment, pathological effects to the gastric epithelial cells and the possible link to Alzheimer's disease. © 2011 Elsevier Ltd.


He G.-N.,Sun Yat Sen University | Huang B.,Jinan University | Shen H.,Sun Yat Sen University
Nanotechnology | Year: 2011

ZnO nanorods were synthesized by a simple aqueous solution method. Crystal structures and morphology studies show that the ZnO nanorods are single crystalline with the growth direction aligned with the c axis of ZnO. An Au-ZnO nanorod-Au (metal-semiconductor-metal, MSM) device using the synthesized nanorod was fabricated. An electronic model with two back-to-back Schottky diodes in series with a nanorod was used to describe the electrical transport of the MSM device. A positive temperature coefficient of resistance is observed on a single ZnO nanorod from 383 to 473 K. A simple model has been proposed to explain such an abnormal behavior including the effect of the interface states and the adsorption-desorption of the water/oxygen molecules on the surface of the nanorod. © 2011 IOP Publishing Ltd.


Guan Z.,Jinan University | Lin Z.,Jinan University | Mai K.,Sun Yat Sen University
Composites Science and Technology | Year: 2013

Calcium carbonate as a filler material provides some β-nucleation capacity for polypropylene in production of particle-reinforced composites. Its effectiveness in this regard mainly depends on the source of calcium carbonate, but the mechanism is not cleared yet. In this study, the prepared Monetaria moneta powder with the main component of calcium carbonate revealed high β-nucleation capacity for polypropylene, which was enhanced with increasing the filler loading with the maximum performance at 5. wt.%. Heat treatment of M. moneta indicated that β-nucleation capability was due to the nano-layer structure of aragonite crystal type in which the lattice parameter has the c-axis perpendicular to the layer and the length of c-axis applied to the dimensional lattice matches the β-phase of PP. The M. moneta powder was observed as a strong β-nucleating agent different from the conventional nucleating agents. © 2013 Elsevier Ltd.


Li B.,Tsinghua University | Huang S.-Q.,Jinan University | Feng X.-Q.,Tsinghua University
Archive of Applied Mechanics | Year: 2010

The wrinkling of a stiff thin film bonded on a soft elastic layer and subjected to an applied or residual compressive stress is investigated in the present paper. A three-dimensional theoretical model is presented to predict the buckling and postbuckling behavior of the film. We obtained the analytical solutions for the critical buckling condition and the postbuckling morphology of the film. The effects of the thicknesses and elastic properties of the film and the soft layer on the characteristic wrinkling wavelength are examined. It is found that the critical wrinkling condition of the thin film is sensitive to the compressibility and thickness of the soft layer, and its wrinkling amplitude depends on the magnitude of the applied or residual in-plane stress. The bonding condition between the soft layer and the rigid substrate has a considerable influence on the buckling of the thin film, and the relative sliding at the interface tends to destabilize the system. © 2009 Springer-Verlag.


Zhong D.-C.,Gannan Normal University | Meng M.,Jinan University | Deng J.-H.,Gannan Normal University | Luo X.-Z.,Gannan Normal University | Xie Y.-R.,Gannan Normal University
Inorganic Chemistry Communications | Year: 2011

A 3D metal-organic framework {[Cd 2(TZ) 3(BDC) 0.5]·5H 2O} n (1·5nH 2O) (HTZ = 1H-tetrazole, H 2BDC = 1,4-benzenedicarboxylic acid), has been hydrothermally synthesized and structurally characterized by single-crystal X-ray diffraction. The phase purity was confirmed by powder X-ray diffraction (PXRD), and the stability was identified by thermal gravimetric analysis (TG) and variable-temperature powder X-ray diffraction (VT-PXRD). The result of the single-crystal X-ray diffraction analysis indicates that 1 is a novel 3D microporous metal-organic framework constructed from Cd(II) metal centers and mixed linkers of TZ - anions and BDC 2- anions. Photoluminescent measurement elucidates that 1 displays a strong and broad emission peak at 423 nm, which suggests that 1 may be a potential purple-light material. © 2011 Elsevier B.V. All rights reserved.


Li J.-M.,Sun Yat Sen University | Wang Y.-Y.,Sun Yat Sen University | Wang Y.-Y.,Jinan University | Zhao M.-X.,Sun Yat Sen University | And 6 more authors.
Biomaterials | Year: 2012

Co-delivery of siRNA and chemotherapeutic agents has been developed to combat multidrug resistance in cancer therapy. Recently, we developed a series of quantum dots (QDs) functionalized by β-cyclodextrin (β-CD) coupled to amino acids, some of which can be used to facilitate the delivery of siRNA. In this study, two CdSe/ZnSe QDs modified with β-CD coupled to L-Arg or L-His were used to simultaneously deliver doxorubicin (Dox) and siRNA targeting the MDR1 gene to reverse the multidrug resistance of HeLa cells. In this co-delivery system, Dox was firstly encapsulated into the hydrophobic cavities of β-CD, resulting in bypass of P-glycoprotein (P-gp)-mediated drug efflux. After complex formation of the mdr1 siRNA with Dox-loaded QDs via electrostatic interaction, significant down-regulation of mdr1 mRNA levels and P-gp expression was achieved as shown by RT-PCR and Western blotting experiments, respectively. The number of apoptotic HeLa cells after treatment with the complexes substantially exceeded the number of apoptotic cells induced by free Dox only. The intrinsic fluorescence of the QDs provided an approach to track the system by laser confocal microscopy. These multifunctional QDs are promising vehicles for the co-delivery of nucleic acids and chemotherapeutics and for real-time tracking of treatment. © 2011 Elsevier Ltd.


Mei X.Q.,Jinan University | Mei X.Q.,Sun Yat Sen University | Wong M.H.,Hong Kong Baptist University | Yang Y.,Jinan University | And 3 more authors.
Environmental Pollution | Year: 2012

Understanding the complex biotic and abiotic interactions invoked by the rice root system in oxygen-depleted soil is an important step in screening genotypes for low toxic metal or metalloid accumulation. A hydroponic and a rhizobox experiment have been conducted to explore the effects of varying root oxygen release on chemical changes, As fractionation in rhizosphere soil and Fe plaque formation, As uptake and tolerance by different rice genotypes. The results showed that rice genotypes with higher rates of radial oxygen loss (ROL) and at the bolting stage, tended to have greater effects on rhizosphere Eh, pH, Fe 3+/Fe 2+ quotients, As fractionation and mobility and also on Fe plaque formation compared to those with lower ROL and at the tillering stage. Genotypes with higher ROL have a strong ability to reduce As accumulation in shoots and increase As tolerance by reducing As mobilization in the rhizosphere and by limiting As translocation. © 2012 Elsevier Ltd. All rights reserved.


Liu Z.-Q.,Jinan University | Liu Z.-Q.,Guangzhou University | Huang W.-Y.,Jinan University | Zhang Y.-M.,Jinan University | Tong Y.-X.,Sun Yat Sen University
CrystEngComm | Year: 2012

The urchin-like Bi 2S 3 spheres and CuS/Bi 2S 3 composite nanowires have been fabricated through a soft chemical route without the assistance of any templates or surfactants. The resulting samples are characterized by scanning electron microscopy, transmission electron microscopy, powder X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and UV-visible diffuse reflectance spectroscopy. The results demonstrate that the morphology, optical and magnetic properties of products exhibit remarkable change owing to copper modification of Bi 2S 3 nanostructures. Furthermore, the CuS/Bi 2S 3 nanostructures display high visible light photoactivities, which can be attributed to the combined effect of copper modification of Bi 2S 3. © 2012 The Royal Society of Chemistry.


Wang Z.-W.,University of Jinan | Wang P.-L.,University of Jinan | Hu C.-Y.,Jinan University
Packaging Technology and Science | Year: 2010

Understanding the diffusion of migrants in polyethylene terephthalate (PET) and calculating the diffusion coefficients are very important for migration research. In this study, the diffusion coefficients of 13 kinds of small molecules with molecular weights ranging from 32 to 339g/mol in amorphous PET are calculated based on molecular dynamics (MD) simulation. By comparison of diffusion coefficients simulated by MD simulation techniques, predicted by the Piringer model and by experiments, the accuracy of the Piringer model and MD simulation techniques for the estimation of diffusion coefficients of migrants in PET is evaluated. The MD simulation shows that Dsimu is very close to Dexp, within one order of magnitude of the experimental diffusion coefficients except for a few samples. The possible reasons for the differences among Dsimu, Dpred and Dexp are analysed from the molecular weight and temperature. The results show that the Piringer-model-predicted values at high temperatures overestimate significantly higher than that at lower temperatures. The activation energy is calculated by the Arrhenius equation, which shows the relationship between diffusion coefficient and temperature. It is shown that the MD simulation yields acceptable activation energy. The study suggests that MD simulation may be a useful approach to calculate the diffusion coefficients of small molecules in PET. Copyright © 2010 John Wiley & Sons, Ltd.


Xing X.,Sun Yat Sen University | Cai W.,Sun Yat Sen University | Shi H.,Sun Yat Sen University | Wang Y.,Jinan University | And 5 more authors.
British Journal of Cancer | Year: 2013

Background: The prognostic value of CDKN2A promoter hypermethylation in colorectal cancer remains controversial. We systematically reviewed the evidence for assessment of CDKN2A methylation in colorectal cancer to elucidate this issue. Methods: Pubmed, Embase and ISI web of knowledge were searched to identify eligible studies to evaluate the association of CDKN2A hypermethylation and overall survival and clinicopathological features of colorectal cancer patients. Combined hazard ratios (HRs) or odds ratios (ORs) with 95% confidence interval (95% CI) were pooled using a random-effects model. Results: A total of 11 studies encompassing 3440 patients were included in the meta-analysis. CDKN2A hypermethylation had an unfavourable impact on OS of patients with colorectal cancer (HR 1.65, 95% CI 1.29-2.11). Subgroup analysis indicated that CDKN2A hypermethylation was significantly correlated with OS in Europe (HR 1.49; 95% CI 1.28-1.74) and Asia (HR 3.30; 95% CI 1.68-6.46). Furthermore, there was a significant association between CDKN2A hypermethylation and lymphovascular invasion (OR 1.68, 95% CI 1.15-2.47), lymph node metastasis (OR 1.68, 95% CI 1.09-2.59) and proximal tumour location (OR 2.09, 95% CI 1.34-3.26) of colorectal cancer. Conclusion: This meta-analysis indicated that CDKN2A hypermethylation might be a predictive factor for unfavourable prognosis of colorectal cancer patients. © 2013 Cancer Research UK. All rights reserved.


Diao Z.,Jinan University | Diao Z.,Sun Yat Sen University | Li M.,Jinan University | Zeng F.,Jinan University | And 2 more authors.
Journal of Hazardous Materials | Year: 2013

A novel dual-tank photoelectrochemical catalytic reactor was designed to investigate the degradation pathway of malachite green. A thermally formed TiO2/Ti thin film electrode was used as photoanode, graphite was used as cathode, and a saturated calomel electrode was employed as the reference electrode in the reactor. In the reactor, the anode and cathode tanks were connected by a cation exchange membrane. Results showed that the decolorization ratio of malachite green in the anode and cathode was 98.5 and 96.5% after 120min, respectively. Malachite green in the two anode and cathode tanks was oxidized, achieving the bipolar double effect. Malachite green in both the anode and cathode tanks exhibited similar catalytic degradation pathways. The double bond of the malachite green molecule was attacked by strong oxidative hydroxyl radicals, after which the organic compound was degraded by the two pathways into 4,4-bis(dimethylamino) benzophenone, 4-(dimethylamino) benzophenone, 4-(dimethylamino) phenol, and other intermediate products. Eventually, malachite green was degraded into oxalic acid as a small molecular organic acid, which was degraded by processes such as demethylation, deamination, nitration, substitution, addition, and other reactions. © 2013 Elsevier B.V.


Nie X.-P.,Jinan University | Liu B.-Y.,Jinan University | Liu B.-Y.,Sun Yat Sen University | Yu H.-J.,Sun Yat Sen University | And 3 more authors.
Environmental Pollution | Year: 2013

We tested antioxidant responses of the green microalga Pseudokirchneriella subcapitata exposed to different concentrations of the three antibiotics erythromycin (ETM), ciprofloxacin (CPF) and sulfamethoxazole (SMZ). Measurements included the level of lipid peroxidation, the total antioxidative capacity and three major antioxidant mechanisms: the ascorbate-glutathione cycle, the xanthophyll cycle and the enzyme activities of catalase (CAT), superoxide dismutase (SOD), guaiacol glutathione peroxidase (GPX) and glutathione-S- transferase (GST). Three antibiotics significantly affect the antioxidant system of P. subcapitata, but in different ways the alga was more tolerant to CPF and SMZ exposures than to ETM exposure. ETM caused reductions in AsA and GSH biosynthesis, ascorbate-glutathione cycle, xanthophylls cycle and antioxidant enzyme activities. The toxicity of CPF seems to be mainly overcome via induction of the ascorbate-glutathione cycle and CAT, SOD and GPX activities, while the toxicity of SMZ on the photosynthetic apparatus is predominantly reduced by the xanthophyll cycle and GST activity. © 2012 Published by Elsevier Ltd.


Sun X.,Jinan University | Ge F.,Jinan University | Xiao C.-L.,Jinan University | Yin X.-F.,Jinan University | And 3 more authors.
Journal of Proteome Research | Year: 2010

Recent phosphoproteomic characterizations of Bacillus subtilis, Escherichia coli, Lactococcus lactis, Pseudomonas putida, and Pseudomonas aeruginosa have suggested that protein phosphorylation on serine, threonine, and tyrosine residues is a major regulatory post-translational modification in bacteria. In this study, we carried out a global and site-specific phosphoproteomic analysis on the Gram-positive pathogenic bacterium Streptococcus pneumoniae. One hundred and two unique phosphopeptides and 163 phosphorylation sites with distributions of 47%/44%/9% for Ser/Thr/Tyr phosphorylations from 84 S. pneumoniae proteins were identified through the combined use of TiO2 enrichment and LC-MS/MS determination. The identified phosphoproteins were found to be involved in various biological processes including carbon/protein/nucleotide metabolisms, cell cycle and division regulation. A striking characteristic of S. pneumoniae phosphoproteome is the large number of multiple species-specific phosphorylated sites, indicating that high level of protein phosphorylation may play important roles in regulating many metabolic pathways and bacterial virulence. © 2010 American Chemical Society.


Yang L.,Jinan University | Liu W.,University of Jinan
IEEE Communications Letters | Year: 2011

In this letter, we consider a single-relay and K users multiple-input multiple-output (MIMO) system with the channel state information (CSI)-assisted amplify-and-forward (CAF) protocol, where each node is equipped with N antennas. We propose one antenna selection scheme for the multiuser wireless relay network. According to the order statistics, we derive the exact expressions for the probability density functions (PDF) and use them to evaluate the average throughput. The numerical results show that for a N=2 and K=3 relay channel the antenna selection scheme results about 2dB SNR loss compared to the full antenna case. © 2011 IEEE.


Chen J.L.,Jinan University | Shan Q.,Minsheng Securities Co
Advanced Materials Research | Year: 2014

The growing natural gas consumption asks for larger scale of pipelines and arouses criticism on separation pipelines business from national oil and gas monopoly companies in China. Through reviewing of America's regulatory practices on access pricing policy in the gas pipeline industry and summarizing the evolution of access pricing theory on network economics, we conclude some suggestions on the reform process of China's pipeline access regulation policy by comparing and contrasting the industrial environments' major differences between China and America. We emphasize the importance of executing non-discrimination access price policy in the spirit of Anti-Monopoly Law and indicate that simple separation isn't the most effective way to increase social welfare nowadays. Regulatory agency should prohibit abusing market power and decrease cost information asymmetric in cross-subsidy activity. © (2014) Trans Tech Publications, Switzerland.


Bakker E.S.,Netherlands Institute of Ecology | Sarneel J.M.,Netherlands Institute of Ecology | Gulati R.D.,Netherlands Institute of Ecology | Liu Z.,CAS Nanjing Institute of Geography and Limnology | And 3 more authors.
Hydrobiologia | Year: 2013

Although many lake restoration projects have led to decreased nutrient loads and increased water transparency, the establishment or expansion of macrophytes does not immediately follow the improved abiotic conditions and it is often unclear whether vegetation with high macrophyte diversity will return. We provide an overview of the potential bottlenecks for restoration of submerged macrophyte vegetation with a high biodiversity and focus on the biotic factors, including the availability of propagules, herbivory, plant competition and the role of remnant populations. We found that the potential for restoration in many lakes is large when clear water conditions are met, even though the macrophyte community composition of the early 1900s, the start of human-induced large-scale eutrophication in Northwestern Europe, could not be restored. However, emerging charophytes and species rich vegetation are often lost due to competition with eutrophic species. Disturbances such as herbivory can limit dominance by eutrophic species and improve macrophyte diversity. We conclude that it is imperative to study the role of propagule availability more closely as well as the biotic interactions including herbivory and plant competition. After abiotic conditions are met, these will further determine macrophyte diversity and define what exactly can be restored and what not. © 2012 The Author(s).


Li W.,Tsinghua University | Fan J.,Jinan University | Mai Y.,Jinan University | Wang L.,Tsinghua University
Advanced Energy Materials | Year: 2016

The stability of single-crystalline/monocrystalline-like perovskite film is expected to be better than its microcrystalline counterparts. In the present work, highly orientated perovskite thin films (CH3NH3PbI3- xClx) are prepared by means of aquointermediates assisted solution process. It displays super-duper preferred-orientation along <110> direction that is close to the single crystal, and its diffraction intensity ratio of (110)/(310) is nearly two orders of magnitude higher in contrast to the films that prepared by traditional way. Owing to its superior performances, e.g., highly crystallized quality, stress-free inside films, longer electron lifetime, faster temporal response time, etc., the highly orientated perovskite-based solar cells accordingly allow realizing high efficiency while improving its thermal stability. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Ma D.,Sun Yat Sen University | Lin J.,Sun Yat Sen University | Lin J.,Guangdong Medical College | Chen Y.,Sun Yat Sen University | And 2 more authors.
Carbon | Year: 2012

Graphene oxide nanosheets were used to induce the in situ gelation of doxorubicin hydrochloride as an antitumor drug. When a very small amount of the graphene oxide was introduced into an aqueous solution of doxorubicin hydrochloride at room temperature, a strong and thixotropic gel was rapidly formed without any polymers or chemical additives. The gelation mechanism was investigated by fluorescence spectroscopy, X-ray diffraction and scanning electron microscopy. The encapsulated doxorubicin hydrochloride was found to show sustained release and antitumor efficacy. © 2012 Elsevier Ltd. All rights reserved.


Wang H.,Rutgers University | Yao K.,King Abdullah University of Science and Technology | Zhang Z.,Jinan University | Jagiello J.,Micromeritics Instrument Co. | And 3 more authors.
Chemical Science | Year: 2014

In industry, cryogenic rectification for separating xenon from other noble gases such as krypton and argon is an energy and capital intensive process. Here we show that a microporous metal-organic framework, namely Co 3(HCOO)6 is capable of effective capture and separation of xenon from other noble gases. Henry's constant, isosteric heat of adsorption (Qst), and IAST selectivity are calculated based on single component sorption isotherms. Having the highest Qst reported to date, Co 3(HCOO)6 demonstrates high adsorption capacity for xenon and its IAST selectivity for Xe-Kr is the largest among all MOFs investigated to date. To mimic real world conditions, breakthrough experiments are conducted on Xe-Kr binary mixtures at room temperature and 1 atmosphere. The results are consistent with the calculated data. These findings show that Co 3(HCOO)6 is a promising candidate for xenon capture and purification. Our gas adsorption measurements and molecular simulation study also reveal that the adsorption of xenon represents the first example of commensurate adsorption of atomic gases near ambient conditions. © 2014 The Royal Society of Chemistry.


Xu L.,Jinan University | Han B.-P.,Jinan University | Van Damme K.,Ghent University | Vierstraete A.,Ghent University | And 2 more authors.
Journal of Biogeography | Year: 2011

Aim To reconstruct the phylogeographic history of the Holarctic carnivorous genus Leptodora (Crustacea: Cladocera: Haplopoda).Location We studied the DNA of between one and five specimens each from 28 populations distributed across the Holarctic, but with emphasis on Eurasia.Methods We sequenced a mitochondrial (cytochrome c oxidase subunit I) and a nuclear (elongation factor-1α) gene, and combined this molecular information with geological and palaeoclimatological data. Haplotype networks and phylogenetic trees were constructed using a Bayesian and maximum likelihood approach. A molecular clock was applied.Results Leptodora consists of three clades (Leptodora kindtii in Europe, Leptodora richardi in China and Japan, and Leptodora sp. in North America), with insular subclades in Japan and in the eastern Mediterranean. The North American clade was not studied in detail. Leptodora richardi is the more thermophilic of the three. It extends from the Tropic of Cancer in the south to the Heilong Basin in the north. The western European L. kindtii is more cold-water adapted than the eastern Mediterranean subclade. 'West European' and 'Chinese' clades are broadly separated by a hybrid zone in Siberia and European Russia as far west as the Volga. These hybrids have the mitochondrial DNA of L. kindtii, the nuclear DNA of L. richardi and the low-temperature preference of L. kindtii, and may have formed as recently as the Holocene hypsithermal. A pure L. kindtii population in the Upper Irtysh catchment, east of the Dzungarian Gates, has been sequestered in endorheic Lake Wulungu, Xinjiang, since the mid-Pleistocene.Main conclusions Application of a molecular clock places the most recent common ancestor of the North American, East Asian and European populations in the mid-Miocene. The North American taxon is still living in isolation, while the Eurasian taxa, separated by the Alpine folding, made contact again in the Pleistocene, when the cold-stenothermic L. kindtii repeatedly moved eastwards across Siberia and back. The population in Xinjiang is a relict of an early wave coming from western Europe: it crossed the Dzungarian Gates during a humid mid-Pleistocene event, probably corresponding to the Apsheron transgression in the Caspian Basin. Later aridity isolated it there, and it started accumulating private haplotypes. The Holocene Euro-Siberian hybrid zone may eventually engulf all European populations. © 2010 Blackwell Publishing Ltd.


Hou D.-F.,Central China Normal University | Liu H.,Jinan University | Ren H.-C.,Rockefeller University
Physical Review D - Particles, Fields, Gravitation and Cosmology | Year: 2012

The two-loop contributions to the chiral vortical conductivity are considered. The Kubo formula together with the anomalous Ward identity of the axial vector current suggest that there may be a nonzero correction to the coefficient of the T2 term of the conductivity. © 2012 American Physical Society.


Hocher B.,University of Potsdam | Hocher B.,Jinan University
Journal of Reproductive Immunology | Year: 2014

Many lines of data, initial epidemiologic studies as well as subsequent extensive experimental studies, indicate that early-life events play a powerful role in influencing later suceptibility to certain chronic diseases. Such events might be over- or undernutrition, exposure to environmental toxins, but also changes in hormones, in particular stress hormones. Typically, those events are triggered by the environmental challenges of the mother. However, recent studies have shown that paternal environmental or nutritional factors affect the phenotype of the offspring as well. The maternal and paternal environmental factors act on the phenotype of the offspring via epigenetic modification of its genome. The advanced fetal programming hypothesis proposes an additional non-environmentally driven mechanism: maternal and also paternal genes may influence the maturating sperm, the oocyte, and later the embryo/fetus, leading to their epigenetic alteration. Thus, the observed phenotype of the offspring may be altered by maternal/paternal genes independent of the fetal genome. Meanwhile, several independent association studies in humans dealing with metabolic and neurological traits also suggest that maternal genes might affect the offspring phenotype independent of the transmission of that particular gene to the offspring. Considering the implications of this hypothesis, some conclusions drawn from transgenic or knockout animal models and based on the causality between a genetic alteration and a phenotype, need to be challenged. Possible implications for the development, diagnostic and therapy of human genetic diseases have to be investigated. © 2014 Elsevier Ireland Ltd.


Luo H.,Jinan University | Wang F.,Biocontrol | Wang F.,National Institutes for Food and Drug Control | Bai Y.,Jinan University | And 2 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2012

In vitro antiproliferative effects of selenium nanoparticles (nanoSe 0, 10-40μmol/L) on HeLa (human cervical carcinoma) cells and MDA-MB-231 (human breast carcinoma) cells were examined by optical microscopic inspection and MTT assay in the present study. The nanoSe 0 effectively inhibited the growth of MDA-MB-231cells and HeLa cells in a dose-dependent manner. The morphology analysis with atomic force microscope showed that the HeLa cells treated with 10μmol/L nanoSe 0 were rough and shrunken with truncated lamellipodia at terminal part of the cells. Flow cytometric analysis demonstrated that HeLa cells were arrested at S phase of the cell cycle after exposed to nanoSe 0 (10μmol/L). Taken together, our results suggested that nanoSe 0 may be more helpful in cancer chemoprevention as a potential anticancer drug. © 2012 Elsevier B.V.


Wang Q.-Q.,Xiangtan University | Wang Q.-Q.,Jinan University | Cheng N.,Xiangtan University | Yi W.-B.,Xiangtan University | And 2 more authors.
Bioorganic and Medicinal Chemistry | Year: 2014

α-Glucosidase (AG) play crucial roles in the digestion of carbohydrates. Inhibitors of α-glucosidase (AGIs) are promising candidates for the development of anti-diabetic drugs. Here, five series of apigenin and chrysin nitric oxide (NO)-donating derivatives were synthesised and evaluated for their AG inhibitory activity and NO releasing capacity in vitro. Except for 9a-c, twelve compounds showed remarkable inhibitory activity against α-glucosidase, with potency being better than that of acarbose and 1-deoxynojirimycin. All organic nitrate derivatives released low concentrations of NO in the presence of l-cysteine. Structure activity relationship studies indicated that 5-OH, hydrophobic coupling chain, and carbonyl groups of the coupling chain could enhance the inhibitory activity. Apigenin and chrysin derivatives therefore represents a new class of promising compounds that can inhibit α-glucosidase activity and supply moderate NO for preventing the development of diabetic complications. © 2014 Elsevier Ltd. All rights reserved.


Sun C.,Jinan University | Sun C.,Lunan Pharmaceutical Group Corporation | Lai X.,Jinan University | Huang X.,Jinan University | Zeng Y.,Jinan University
Biological and Pharmaceutical Bulletin | Year: 2014

Ginsenoside Rg1 (Rg1), one of the active ingredients in Panax ginseng, has been known to regulate many cellular processes. The purpose of this study was to investigate the protective effects of Rg1 on apoptosis in mouse cultured astrocytes in vitro and a mouse model of cerebral ischemia and reperfusion in vivo. The cell apoptosis was measured by fluorescence microplate reader and xCELLigence system and the Ca2+ overload was recorded by confocal microscopy. The mitochondrial membrane potential and reactive oxygen species (ROS) were determined by flow cytometry. BALB/c mice were subjected to transient middle cerebral artery occlusion (MCAO) and randomly divided into four groups: Sham (sham-operated +0.9% saline), MCAO (MCAO+0.9% saline), Rg1-L (MCAO+Rg1 20 mg/kg) and Rg1-H (MCAO+Rg1 40 mg/kg). Neurological deficit scores, brain water content and infarct volume were evaluated at 24 h after reperfusion. The results showed that Rg1 significantly attenuated H2O2-induced apoptosis in astrocytes. Rg1 efficiently inhibited intracellular Ca2+ overload, loss of mitochondrial membrane potential, and ROS production in astrocytes. In vivo study, it was also observed that Rg1 markedly reduced the neurological deficit scores, brain edema, and infarct volume in the model mice. These results suggest that Rg1 possesses significant neuroprotective effects, which might be related to the prevention of astrocytes from apoptosis. © 2014 The Pharmaceutical Society of Japan.


Dumont H.J.,Ghent University | Dumont H.J.,Jinan University
Odonatologica | Year: 2013

COI and ITS DNA fragments were used to gain insight into the phylogenetic relationships within Ischnura. The genus is recovered as monophyletic, and the 24 species-level taxa considered (about one third of the total) suggest the existence of 2 main clades, here called the I. elegans and I pumilio groups. Each group has a core number of about 4-5 spp. while the placement of most others is not well resolved and will require further study. However, for a number of taxa, their relationship within the species group is clarified. This is true of formerly enigmatic spp. like I. aralensis, but also of I. fountaineae, I. evansi, and others. I. aurora is found to be only distantly related to I. rubilio and both certainly deserve full species status and occupy disjunct geographic ranges. Ischnura nursei is confirmed as a true Ischnura. I. graellsi and I. saharensis, although closely related to I. elegans, appear to be good species.


Tang S.,Jinan University | Xu W.,Tianjin Hospital
Iranian Red Crescent Medical Journal | Year: 2012

Background: The restoration of disc space height (DSH) is essential in anterior lumbar interbody fusion (ALIF), while it is unclear whether the reduction of DSH may alter the mechanical status and adversely affect adjacent segment, and few literatures focused on the subject. Methods: Ninety five patients who had undergone ALIF for degenerative disc disease at our institution between March 2004 and March 2007 were retrospectively reviewed and 76 patients were enrolled in this study. Preoperative, postoperative and the final follow-up segmental lordosis (SL), whole lumbar lordosis (WLL) and DSH were measured and compared in adjacent segmental degeneration (ASD) group and non-ASD group, and the relationship between DSH, SL, WLL and ASD were investigated retrospectively. Results: In 76 patients, the radiographic ASD was proven in 25 (32.9%) and symptomatic ASD in 2 patients. There was a significant correlation between DSH and SL, but was insignificant between DSH and WLL, and a significant correlation was noticed between ASD and SL, WLL and DSH at final follow-up. Conclusion: The normal DSH and SL is important for preventing ASD and an anterior cage with appropriate height and lordotic angle to be used in ALIF to maintain the proper DSH and SL. © Iranian Red Crescent Medical Journal.


Dumont H.J.,Jinan University | Dumont H.J.,Ghent University
Odonatologica | Year: 2014

Fourteen species of Odonata were collected in Ounianga and Tibesti (Chad, Af-rica) in March 2014. Among them, only one zygopteran (Ischnura saharensis, with a Saharan distribution), one gomphid (the eremian Paragomphus sinaiticus), and two aeshnids (Anax imperator and Hemianax ephippiger) were present. The latter species was not only the most common dragonfly, but also the most abundant insect seen in the desert. It is likely that it was in a phase of accumulating individuals, possibly as a prelude to another massive trans-Sahara and even trans-Mediterranean migration, for which the species is well-known. The 10 libellulids recorded were almost all Afrotropical species, but several expand to the Maghreb and even Mediterranean Europe. Only Orthetrum cf. hintzi is a tropical African species that had never been recorded from the desert before.


Gu B.,Jinan University | Gu B.,South Florida Water Management District | Schelske C.L.,University of Florida | Waters M.N.,Valdosta State University
Oecologia | Year: 2011

Carbon stable isotopes (δ 13C) of particulate organic matter (POM) have been used as indicators for energy flow, primary productivity and carbon dioxide concentration in individual lakes. Here, we provide a synthesis of literature data from 32 freshwater lakes around the world to assess the variability of δ 13C POM along latitudinal, morphometric and biogeochemical gradients. Seasonal mean δ 13C POM, a temporally integrated measure of the δ 13C POM, displayed weak relationships with all trophic state indices [total phosphorus (TP), total nitrogen (TN), and chlorophyll a (Chl a)], but decreased significantly with the increase in latitude, presumably in response to the corresponding decrease in water temperature and increase in CO 2 concentration. The seasonal minimum δ 13C POM also correlated negatively with latitude while seasonal maximum δ 13C POM correlated positively with all trophic state indices, pH, and δ 13C of dissolved inorganic carbon (DIC). Seasonal amplitude of δ 13C POM (the difference between seasonal maximum and minimum values) correlated significantly with pH, TP and Chl a concentrations and displayed small variations in oligotrophic, mesotrophic and low latitude eutrophic lakes, which is attributed to low primary productivity and abundant non-living POM in the low trophic state lakes and relatively stable environmental conditions in the subtropics. Seasonal amplitude of δ 13C POM was the greatest in high latitude eutrophic lakes. Greater seasonal changes in solar energy and light regime may be responsible for the large seasonal variability in high latitude productive lakes. This synthesis provides new insights on the factors controlling variations in stable carbon isotopes of POM among lakes on the global scale. © 2010 Springer-Verlag.


Hou D.-F.,Central China Normal University | Liub H.,Jinan University | Renc H.-C.,Central China Normal University | Renc H.-C.,Rockefeller University
Journal of High Energy Physics | Year: 2011

In this paper, we shall address some field theoretic issues regarding the chiral magnetic effect. The general structure of the chiral magnetic current consistent with the electromagnetic gauge invariance is obtained and the impact of the infrared divergence is examined. Some subtleties on the relation between the chiral magnetic effect and the axial anomaly are clarified through a careful examination of the infrared limit of the relevant thermal diagrams. © SISSA 2011.


Yu S.,Jinan University | Geng Q.,Jinan University | Ma J.,Shanghai 10th Peoples Hospital | Sun F.,Peoples 10th Hospital | And 3 more authors.
Cell Death and Disease | Year: 2013

Osteoblast differentiation is a pivotal event in bone formation. Runt-related transcription factor-2 (Runx2) is an essential factor required for osteoblast differentiation and bone formation. However, the underlying mechanism of Runx2-regulated osteogenic differentiation is still unclear. Here, we explored the corresponding mechanism using the C2C12/Runx2Dox subline, which expresses Runx2 in response to doxycycline (Dox). We found that Runx2-induced osteogenic differentiation of C2C12 cells results in a sustained decrease in the expression of heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family. Forced expression of HB-EGF or treatment with HB-EGF is capable of reducing the expression of alkaline phosphatase (ALP), a defined marker of early osteoblast differentiation. HB-EGF-mediated inhibition of ALP depends upon activation of the EGFR and the downstream extracellular signal-regulated kinase, c-Jun N-terminal kinase mitogen-activated protein kinase pathways as well as phosphatidylinositol 3-kinase/Akt pathway. Runx2 specifically binds to the Hbegf promoter, suggesting that Hbegf transcription is directly inhibited by Runx2. Runx2 can upregulate miR-1192, which enhances Runx2-induced osteogenic differentiation. Moreover, miR-1192 directly targets Hbegf through translational inhibition, suggesting enhancement of Runx2-induced osteogenic differentiation by miR-1192 through the downregulation of HB-EGF. Taken together, our results suggest that Runx2 induces osteogenic differentiation of C2C12 cells by inactivating HB-EGF-EGFR signaling through the downregulation of HB-EGF via both transcriptional and post-transcriptional mechanisms. © 2013 Macmillan Publishers Limited. All rights reserved.


Fedyunin I.,University of Potsdam | Fedyunin I.,Max Planck Institute of Colloids and Interfaces | Lehnhardt L.,University of Potsdam | Bohmer N.,University of Potsdam | And 4 more authors.
FEBS Letters | Year: 2012

Clusters of codons pairing to low-abundance tRNAs synchronize the translation with co-translational folding of single domains in multidomain proteins. Although proven with some examples, the impact of the ribosomal speed on the folding and solubility on a global, cell-wide level remains elusive. Here we show that upregulation of three low-abundance tRNAs in Escherichia coli increased the aggregation propensity of several cellular proteins as a result of an accelerated elongation rate. Intriguingly, alterations in the concentration of the natural tRNA pool compromised the solubility of various chaperones consequently rendering the solubility of some chaperone-dependent proteins. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.


Zhang G.,University of Potsdam | Zhang G.,Jinan University | Fedyunin I.,University of Potsdam | Fedyunin I.,Max Planck Institute of Colloids and Interfaces | And 4 more authors.
Nucleic Acids Research | Year: 2012

The most crucial step in data processing from high-throughput sequencing applications is the accurate and sensitive alignment of the sequencing reads to reference genomes or transcriptomes. The accurate detection of insertions and deletions (indels) and errors introduced by the sequencing platform or by misreading of modified nucleotides is essential for the quantitative processing of the RNA-based sequencing (RNA-Seq) datasets and for the identification of genetic variations and modification patterns. We developed a new, fast and accurate algorithm for nucleic acid sequence analysis, FANSe, with adjustable mismatch allowance settings and ability to handle indels to accurately and quantitatively map millions of reads to small or large reference genomes. It is a seed-based algorithm which uses the whole read information for mapping and high sensitivity and low ambiguity are achieved by using short and non-overlapping reads. Furthermore, FANSe uses hotspot score to prioritize the processing of highly possible matches and implements modified SmithWatermann refinement with reduced scoring matrix to accelerate the calculation without compromising its sensitivity. The FANSe algorithm stably processes datasets from various sequencing platforms, masked or unmasked and small or large genomes. It shows a remarkable coverage of low-abundance mRNAs which is important for quantitative processing of RNA-Seq datasets. © 2012 The Author(s).


Ai B.-Q.,South China Normal University | Zhong W.-R.,Jinan University | Hu B.,Hong Kong Baptist University | Hu B.,University of Houston
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2011

We study heat conduction through one-dimensional homogeneous lattices in the presence of the nonlinear on-site potentials containing the bounded and unbounded parts, and the harmonic interaction potential. We observe the occurrence of double negative differential thermal resistance (NDTR); namely, there exist two regions of temperature difference, where the heat flux decreases as the applied temperature difference increases. The nonlinearity of the bounded part contributes to NDTR at low temperatures and NDTR at high temperatures is induced by the nonlinearity of the unbounded part. The nonlinearity of the on-site potentials is necessary to obtain NDTR for the harmonic interaction homogeneous lattices. However, for the anharmonic homogeneous lattices, NDTR even occurs in the absence of the on-site potentials, for example, the rotator model. © 2011 American Physical Society.


Xu A.-D.,Jinan University | Wang Y.-J.,Capital Medical University | Wang D.Z.,Illinois College
CNS Neuroscience and Therapeutics | Year: 2013

Background: The last update of the consensus statement on intravenous recombinant tissue plasminogen activator (IV rt-PA) for acute ischemic stroke (AIS) by the Chinese Stroke Therapy Expert Panel was published in 2006. Great progress has been made since then. Aim: To provide another update on the new knowledge of IV rt-PA for AIS since 7 years ago. Method: In summer of 2012, the Chinese Stroke Therapy Expert Panel was reconvened. New publications on the use of IV rt-PA for AIS were reviewed. In addition, all newly published consensus and guidelines from other countries were reviewed. The 2006 version of Chinese Consensus was then updated. Results: There is now clinical evidence to support the use of IV rt-PA between 3 and 4.5 h after the onset with several exclusion criteria. More studies are needed to provide the evidence for IV rt-PA use beyond 4.5 h. There is benefit giving IV rt-PA within 3 h to patients who are older than 80 and in patients with ongoing atrial fibrillation. Patients with INR<1.7 while on warfarin, minor strokes, rapid improving strokes and severe strokes should be treated and can all be benefited from IV rt-PA. Discussion: Since IV rt-PA was initially recommended in 1996, there is now more evidence support its use, efficacy and safety. The treatment time window is also being expanded. More public education on stroke recognition are needed so many stroke patients may benefit from the treatment. Conclusion: The 2013 version of Chinese IV rt-PA consensus contains the most up-to-date information on the use of IV rt-PA for AIS. It will be a useful tool and guideline to provide appropriate thrombolytic therapy to stroke patients who meet the criteria. © 2013 John Wiley & Sons Ltd.


Yang J.,Jinan University | Chan E.H.W.,Charles Darwin University | Wang X.,Jinan University | Feng X.,Jinan University | Guan B.,Jinan University
Optics Express | Year: 2015

An all-optical photonic microwave phase shifter that can realize a continuous 360° phase shift over a wide frequency range is presented. It is based on the new concept of controlling the amplitude and phase of the two RF modulation sidebands via a Fourier-domain optical processor. The operating frequency range of the phase shifter is largely increased compared to the previously reported Fourier-domain optical processor based phase shifter that uses only one RF modulation sideband. This is due to the extension of the lower RF operating frequency by designing the amplitude and phase of one of the RF modulation sidebands while the other sideband is designed to realize the required RF signal phase shift. The two-sideband amplitude-and-phase-control based photonic microwave phase shifter has a simple structure as it only requires a single laser source, a phase modulator, a Fourier-domain optical processor and a single photodetector. Investigation on the bandwidth limitation problem in the conventional Fourier-domain optical processor based phase shifter is presented. Comparisons between the measured phase shifter output RF amplitude and phase responses with theory, which show excellent agreement, are also presented for the first time. Experimental results demonstrate the full -180° to + 180° phase shift with little RF signal amplitude variation of less than 3 dB and with a phase deviation of less than 4° over a 7.5 GHz to 26.5 GHz frequency range, and the phase shifter exhibits a long term stable performance. © 2015 Optical Society of America.


Wang X.,Jinan University | Yang J.,Jinan University | Chan E.H.W.,Charles Darwin University | Feng X.,Jinan University | Guan B.-O.,Jinan University
Optics Letters | Year: 2015

An all-optical photonic microwave phase shifter that can realize a continuous 0°-360° phase shift is presented. The phase-shifting operation is implemented by controlling the phase of the two RF phase-modulation sidebands while keeping the optical carrier phase fixed. The use of two RF modulation sidebands, instead of a single sideband used in most conventional phase shifters, has the advantage of highoutput RF signal power, and consequently high signal-tonoise ratio performance. Experimental results demonstrate the full -180° to -180° phase shift over a wide microwave frequency range from 11 to 26.5 GHz, and 14 dB increase in the output RF signal power compared to a conventional phase shifter. © 2015 Optical Society of America.


Ai B.-Q.,South China Normal University | He Y.-F.,Hebei University | Zhong W.-R.,Jinan University
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2010

Directed transport of overdamped Brownian particles driven by fractional Gaussian noises is investigated in asymmetrically periodic potentials. By using Langevin dynamics simulations, we find that rectified currents occur in the absence of any external driving forces. Unlike white Gaussian noises, fractional Gaussian noises can break thermodynamical equilibrium and induce directed transport. Remarkably, the average velocity for persistent fractional noise is opposite to that for antipersistent fractional noise. The velocity increases monotonically with Hurst exponent for the persistent case, whereas there exists an optimal value of Hurst exponent at which the velocity takes its maximal value for the antipersistent case. © 2010 The American Physical Society.


Wu C.,Hong Kong Polytechnic University | Wu C.,Dalian University of Technology | Fu H.Y.,Hong Kong Polytechnic University | Qureshi K.K.,King Fahd University of Petroleum and Minerals | And 2 more authors.
Optics Letters | Year: 2011

A fiber-optic Fabry-Perot interferometer was constructed by splicing a short length of photonic crystal fiber to a standard single-mode fiber. The photonic crystal fiber functions as a Fabry-Perot cavity and serves as a direct sensing probe without any additional components. Its pressure and temperature responses in the range of 0-40 MPa and 25 °C-700 °C were experimentally studied. The proposed sensor is easy to fabricate, potentially lowcost, and compact in size, which makes it very attractive for high-pressure and high-temperature sensing applications. © 2011 Optical Society of America.


Li W.-R.,Guangdong Institute of Microbiology | Li W.-R.,Jinan University | Xie X.-B.,Guangdong Institute of Microbiology | Shi Q.-S.,Guangdong Institute of Microbiology | And 3 more authors.
BioMetals | Year: 2011

The antibacterial activity and mechanism of silver nanoparticles (Ag-NPs) on Staphylococcus aureus ATCC 6538P were investigated in this study. The experiment results showed the minimum bactericidal concentration (MBC) of Ag-NPs to S. aureus was 20 μg/ml. Moreover, when bacteria cells were exposed to 50 μg/ml Ag-NPs for 6 h, the cell DNA was condensed to a tension state and could have lost their replicating abilities. When S. aureus cells were exposed to 50 μg/ml Ag-NPs for 12 h, the cell wall was breakdown, resulting in the release of the cellular contents into the surrounding environments, and finally became collapsed. And Ag-NPs could reduce the enzymatic activity of respiratory chain dehydrogenase. Furthermore, the proteomic analysis showed that the expression abundance of some proteins was changed in the treated bacterial cell with Ag-NPs, formate acetyltransferase increased 5.3-fold in expression abundance, aerobic glycerol-3-phosphate dehydrogenase decreased 6.5-fold, ABC transporter ATP-binding protein decreased 6.2-fold, and recombinase A protein decreased 4.9-fold. © 2010 Springer Science+Business Media, LLC.


Ai B.-Q.,South China Normal University | He Y.-F.,Hebei University | Zhong W.-R.,Jinan University
Soft Matter | Year: 2015

Dynamics and separation of mixed chiral microswimmers are numerically investigated in a channel with regular arrays of rigid half-circle obstacles. For zero shear flow, transport behaviors are the same for different chiral particles: the average velocity decreases with increase of the rotational diffusion coefficient, the direction of the transport can be reversed by tuning the angular velocity, and there exists an optimal value of the packing fraction at which the average velocity takes its maximal value. However, when the shear flow is considered, different chiral particles show different behaviors. By suitably tailoring parameters, particles with different chiralities can move in different directions and can be separated. In addition, we also proposed a space separation method by introducing a constant load, where counterclockwise and clockwise particles stay in different regions of the channel. © The Royal Society of Chemistry 2015.


Li W.-R.,Jinan University | Li W.-R.,Guangdong Institute of Microbiology | Shi Q.-S.,Guangdong Institute of Microbiology | Ouyang Y.-S.,Guangdong Institute of Microbiology | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2013

Essential oils are aromatic oily liquids obtained from some aromatic plant materials. Certain essential oils such as citronella oil contain antifungal activity, but the antifungal effect is still unknown. In this study, we explored the antifungal effect of citronella oil with Aspergillus niger ATCC 16404. The antifungal activity of citronella oil on conidia of A. niger was determined by poisoned food technique, broth dilution method, and disc volatility method. Experimental results indicated that the citronella oil has strong antifungal activity: 0.125 (v/v) and 0.25 % (v/v) citronella oil inhibited the growth of 5 × 105 spore/ml conidia separately for 7 and 28 days while 0.5 % (v/v) citronella oil could completely kill the conidia of 5 × 10 5 spore/ml. Moreover, the fungicidal kinetic curves revealed that more than 90 % conidia (initial concentration is 5 × 105 spore/ml) were killed in all the treatments with 0.125 to 2 % citronella oil after 24 h. Furthermore, with increase of citronella oil concentration and treatment time, the antifungal activity was increased correspondingly. The 0.5 % (v/v) concentration of citronella oil was a threshold to kill the conidia thoroughly. The surviving conidia treated with 0.5 to 2 % citronella oil decreased by an order of magnitude every day, and no fungus survived after 10 days. With light microscope, scanning electron microscope, and transmission electron microscope, we found that citronella oil could lead to irreversible alteration of the hyphae and conidia. Based on our observation, we hypothesized that the citronella oil destroyed the cell wall of the A. niger hyphae, passed through the cell membrane, penetrated into the cytoplasm, and acted on the main organelles. Subsequently, the hyphae was collapsed and squashed due to large cytoplasm loss, and the organelles were severely destroyed. Similarly, citronella oil could lead to the rupture of hard cell wall and then act on the sporoplasm to kill the conidia. Nevertheless, the citronella oil provides a potential of being a safe and environmentally friendly fungicide in the future. © 2012 Springer-Verlag Berlin Heidelberg.


Cheng Z.,Hong Kong Baptist University | Man Y.B.,Hong Kong Baptist University | Nie X.P.,Jinan University | Wong M.H.,Hong Kong Baptist University | Wong M.H.,City University of Hong Kong
Chemosphere | Year: 2013

Cadmium, lead, zinc, Chromium, copper, nickel and manganese in sediments and in aquatic organisms were collected from the aquaculture pond ecosystem of the Pearl River Delta (PRD), China and analyzed to evaluate bioaccumulation and trophic transfer in food chains, as well as the potential health risk of exposure to the Hong Kong residents via dietary intake of these aquatic products. The results revealed that based on the biota-sediment accumulation factor, omnivorous fish and zooplankton accumulated more trace metals from sediment than carnivorous fish. Concentrations of seven trace metals in aquaculture pond of PRD significantly decreased with increasing trophic levels, showing that these trace metals were trophically diluted in predatory and omnivorous food chains. The hazard index values of all fish species were smaller than 1 for adults and children, indicating there was no health risk from the multiple metals via ingestion of the freshwater fish for the inhabitants. © 2012 Elsevier Ltd.


Wang H.,Jinan University | Cai H.-H.,Jinan University | Zhang L.,Jinan University | Cai J.,Jinan University | And 2 more authors.
Biosensors and Bioelectronics | Year: 2013

A rapid, easy assay for monitoring dynamics of T-cell activation should help to guide potential medical evaluation of immune responses or immunopathogenesis. Here, we report development of novel electrochemical cytosensors for dynamic analyses of T-cell activation markers on living cells. Gold nanoparticles-doped polyaniline nanofiber (Au/PANI-NFs) composite was greenly prepared by in situ one-step chemical inertness of PANI-NFs with gold nanoparticles to fabricate impedance-based electrochemical biosensors. Transmission electron micrographs indicated that the gold nanoparticles were uniformly anchored along with the structure of PANI-NF surface, displaying fibrillar morphology with a ~60nm diameter. Au/PANI-NFs-based cytosensors coated with anti-CD Ab molecules could provide biomimetic interface for multiple immunosensing of T-cell surface activation markers (CD69, CD25, and CD71). The dual signal amplification of Au nanoparticle and PANI-NFs-based electrochemical impedance spectroscopic (EIS) measurements enabled the cytosensors considerably sensitive, with a detection limit of 1×104 cells/ml of activated T-cells. The activation-targeted cytosensors detected early, middle and late stages for expression of activation markers CD69, CD25, and CD71 at 8h, 24h, and 36h, respectively, after concanvalin A stimulation of T cells. The quantitative results consisted with those derived from flow cytometric analysis. Furthermore, activation-targeted cytosensor allowed for dynamic analysis of the immune inhibition of T-cell activation by immune regulatory drug icariin (ICA). Thus, Au/PANI-NFs-based cytosensors offer simple and fast approach for non-destructive, quantitative evaluation of T-cell activation markers, with considerable specificity, reproducibility, and low background noise. A rapid, easy assay for monitoring dynamics of T-cell activation. Constructions of biosensor interface of gold nanoparticles-doped polyaniline nanofiber composite. A detection limit of activated T-cells for activation-targeted cytosensor at 1×104 cells/ml. © 2013 The Authors.


Ai B.-Q.,South China Normal University | He Y.-F.,Hebei University | Zhong W.-R.,Jinan University
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2011

Transport of Brownian particles interacting with each other via the Morse potential is investigated in the presence of an ac driving force applied locally at one end of the chain. By using numerical simulations, we find that the system can behave as a particle diode for both overdamped and underdamped cases. For low frequencies, the transport from the free end to the ac acting end is prohibited, while the transport from the ac acting end to the free end is permitted. However, the polarity of the particle diode will reverse for medium frequencies. There exists an optimal value of the well depth of the interaction potential at which the average velocity takes its maximum. The average velocity υ decreases monotonically with the system size N by a power law υN-1. © 2011 American Physical Society.


Wu L.,Shandong University | Man C.,Jining Hospital of Infectious Diseases | Wang H.,Shandong Provincial Qianfoshan Hospital | Lu X.,Shandong Academy of Sciences | And 3 more authors.
Pharmaceutical Research | Year: 2013

Purpose: To develop PEGylated multi-walled carbon nanotubes as a sustained release drug delivery system. Methods: Oxaliplatin was incorporated into inner cavity of PEGylated multi-walled carbon nanotubes (MWCNT-PEG) using nano-extraction. Oxaliplatin release rates from MWCNT-PEG-Oxaliplatin were investigated using dialysis tubing. Cytotoxicity of oxaliplatin, MWCNT-Oxaliplatin and MWCNT-PEG-Oxaliplatin were evaluated in HT29 cell by MTT assay, Pt-DNA adducts formation, γ-H2AX formation and cell apoptosis assay. Results: Loading of oxaliplatin into MWCNT-PEG was ~43.6%. Sustained release occurred to MWCNT-PEG-Oxaliplatin, with only 34% of oxaliplatin released into medium within 6 h. In MTT assay, MWCNT-PEG-Oxaliplatin showed slightly decreased cytotoxic effect when cell viability was assessed at 12 and 24 h. A drastic increase of cytotoxicity was found when cell viability was assessed at 48 and 96 h. Pt-DNA adducts formation, γ-H2AX formation and cell apoptosis assay results showed the same trend as the MTT assay, suggesting sustained-release for MWCNT-Oxaliplatin and MWCNT-PEG-Oxaliplatin formulations. Conclusions: PEGylated multi-walled carbon nanotubes can be used as sustained release drug delivery system, thus remarkably improving cytotoxicity of oxaliplatin on HT-29 cells. © 2012 Springer Science+Business Media, LLC.


Weber-Boyvat M.,Minerva Foundation Institute for Medical Research | Zhong W.,Jinan University | Yan D.,Jinan University | Olkkonen V.M.,Minerva Foundation Institute for Medical Research | Olkkonen V.M.,University of Helsinki
Biochemical Pharmacology | Year: 2013

Oxysterol-binding (OSBP)-related proteins (ORPs) constitute a family of sterol and phosphoinositide binding/transfer proteins in eukaryotes from yeast to man. While their functions have mainly been addressed in cellular lipid metabolism or sterol transport, increasing evidence points to more versatile regulatory roles in a spectrum of cellular regimes. In fact ORPs do not appear to be robust controllers of lipid homeostasis. Several ORPs localize at membrane contacts sites (MCS), where endoplasmic reticulum (ER) is apposed with other organelle limiting membranes. Apparently, ORPs have the capacity to control the formation of MCS or activity of enzymatic machineries at these sites. Thereby, ORPs most likely affect organelle membrane lipid compositions, with impacts on signaling and vesicle transport, but also cellular lipid metabolism. Moreover, an increasing number of protein interaction partners of ORPs have been identified, connecting these proteins with various aspects of cell regulation. Small molecular anti-proliferative compounds, ORPphilins, were recently found to target two members of the ORP family, OSBP and ORP4, revealing an essential function of ORPs in cancer cell proliferation and survival. Further functions assigned for ORPs include regulation of extracellular signal regulated kinase (ERK) activity (OSBP), control of ER-late endosome MCS and late endosome motility (ORP1L), regulation of b1-integrin activity (ORP3), modulation of hepatocyte insulin signaling and macrophage migration (ORP8), as well as post-Golgi vesicle transport, phosphatidylinositol-4-phosphate and target of rapamycin complex 1 signaling and nitrogen sensing (Saccharomyces cerevisiae Osh4p). These and other recent observations shed light on the ORPs as integrators of lipid signals with an unforeseen variety of vital cellular processes. © 2013 Elsevier Inc. All rights reserved.


Ai B.-Q.,South China Normal University | Chen Q.-Y.,South China Normal University | He Y.-F.,Hebei University | Li F.-G.,South China Normal University | Zhong W.-R.,Jinan University
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2013

Rectification and diffusion of noninteracting self-propelled particles is numerically investigated in a two-dimensional corrugated channel. From numerical simulations, we obtain the average velocity and the effective diffusion coefficient. It is found that the self-propelled particles can be rectified by the self-propelled velocity. There exist optimal values of the parameters (the self-propelled velocity, the translational diffusion constant, and the height of the potential) at which the average velocity takes its maximal value. There exists an optimal translational diffusion at which the effective diffusion constant is maximal. The self-propelled velocity can strongly increase the effective diffusion, while the large rotational diffusion rate can strongly suppress the effective diffusion. © 2013 American Physical Society.


Rong J.,Jinan University | Niu Z.,CAS Technical Institute of Physics and Chemistry | Lee L.A.,University of South Carolina | Wang Q.,University of South Carolina
Current Opinion in Colloid and Interface Science | Year: 2011

Viruses, as typical bionanoparticles from nature, possess many superb properties, such as exquisite symmetry, uniformity of size and shape, well-characterized surface chemistry that governs the potential interparticle interactions. These features make viral particles ideal building blocks for self-assembly studies and novel materials development. This review outlines some of the recent research activities in the area of controlled self-assembly of viruses and its potential use as materials. One particular application of the assembled viruses, especially for biomaterials, is also briefly discussed. © 2011 Elsevier Ltd.


Su H.-C.,CAS Guangzhou Institute of Geochemistry | Ying G.-G.,CAS Guangzhou Institute of Geochemistry | Tao R.,CAS Guangzhou Institute of Geochemistry | Tao R.,Jinan University | And 3 more authors.
Environmental Pollution | Year: 2012

Antibiotic susceptibility, detection of sul gene types and presence of class 1, 2 and 3 integrons and gene cassettes using PCR assays were investigated in 3456 Escherichia coli isolates obtained from 38 sampling sites of the Dongjiang River catchment in the dry and wet seasons. 89.1% of the isolates were resistant and 87.5% showed resistance to at least three antibiotics. sul2 was detected most frequently in 89.2% of 1403 SXT-resistant isolates. The presence of integrons (class 1 and 2) was frequently observed (82.3%) while no class 3 integron was found. In these integrons, 21 resistance genes of 14 gene cassette arrays and 10 different families of resistance genes were identified. Three gene cassette arrays, aac(6')-Ib-cr-aar-3-dfrA27-aadA16, aacA4-catB3-dfrA1 and aadA2-lnuF, were detected for the first time in surface water. The results showed that bacterial resistance in the catchment was seriously influenced by human activities, especially discharge of wastewater. © 2012 Elsevier Ltd. All rights reserved.


Li J.,Jinan University | Wu C.,Jinan University | Zhang Q.,Jinan University | Yan B.,CAS Guangzhou Institute of Geochemistry
Dalton Transactions | Year: 2013

Density functional theory calculations have been carried out to study the reaction mechanism of the [FeIII(F20TPP)Cl] catalyzed C-H amination reaction. The calculations show that the classical three-step mechanism for other metals (Ru, Rh, Ir and Zn), including N2 liberation, C-N bond formation and 1,2-hydrogen shift, does not fit the iron(iii)-catalyzed system. After N2 liberation, the favorable reaction pathway for the iron(iii)-catalyzed system is a 1,2-hydrogen shift preceding C-N bond formation, i.e., a H-abstraction/radical rebound mechanism. © 2013 The Royal Society of Chemistry.


Fu Y.-W.,Jinan University | Zhang Q.-Z.,Jinan University | Xu D.-H.,U.S. Department of Agriculture | Liang J.-H.,Jinan University | Wang B.,Jinan University
Journal of Agricultural and Food Chemistry | Year: 2014

Ichthyophthirius multifiliis (Ich), a fish ectoparasite, comprises an important challenge in the aquaculture industry. In this study, a steroidal glycoside, cynatratoside-C, isolated from Cynanchum atratum roots by bioassay-guided fractionation was used to treat I. multifiliis. The cynatratoside-C at 0.25 mg/L demonstrated a 100% mortality of I. multifiliis in vitro after 5 h exposure. The 5 h median effective concentration (EC 50) of cynatratoside-C to nonencysted tomonts was 0.083 mg/L. In addition, cynatratoside-C at concentrations of 0.125 and 0.06 mg/L could completely terminate the reproduction of encysted tomonts. The cynatratoside-C at 2 mg/L could cure the infected grass carp within 48 h. The exact mechanism of cynatratoside-C for killing I. multifiliis is unknown, but it manifests itself microscopically through loss of membrane integrity of nonencysted tomonts or through releasing immature theronts from encysted tomonts. The immature theronts finally died before infecting fish. On the basis of these results, cynatratoside-C could be used as a natural anti-I. multifiliis agent. © 2014 American Chemical Society.


Wu C.,Jinan University | Li J.,Jinan University | Yan B.,CAS Guangzhou Institute of Geochemistry
Dalton Transactions | Year: 2014

A systematic DFT study was performed to examine the isomerization of 2-aryl-2H-azirines to 2,3-disubstituted indoles by FeCl2 and Rh 2(O2CCF3)4. The results indicate that the isomerization of 2-aryl-2H-azirines mainly proceeds through a stepwise mechanism and the Rh2(O2CCF3)4 exhibits higher catalytic performance than FeCl2. Investigation of the magnetic properties suggests that the C-N bond formation step is pseudoelectrocyclization for the FeCl2-catalyzed system. The calculations show that a water-catalyzed 1,2-H shift for the FeCl 2-catalyzed system adopts a proton-transport catalysis strategy, in which chlorine atom coordination to the iron center is critical because it acts as a proton acceptor. When a molecule of water is involved in the Rh 2(O2CCF3)4-catalyzed reaction, the 1,2-H shift is significantly promoted, so that the rate-determining step becomes the ring opening of 2-aryl-2H-azirine. In addition, we studied the catalytic activity of Fe(OAc)2 and CuCl. This journal is © the Partner Organisations 2014.


Zhang D.D.,University of Hong Kong | Lee H.F.,University of Hong Kong | Wang C.,Jinan University | Li B.,South China Normal University | And 3 more authors.
Global Ecology and Biogeography | Year: 2011

Aim It has long been assumed that deteriorating climate (cooling and warming above the norm) could shrink the carrying capacity of agrarian lands, depriving the human population of sufficient food. Population collapses (i.e. negative population growth) follow. However, this human-ecological relationship has rarely been verified scientifically, and evidence of warming-caused disaster has never been found. This research sought to explore quantitatively the temporal pattern, spatial pattern and triggers of population collapses in relation to climate change at the global scale over 1100 years. Location Various countries/regions in the Northern Hemisphere (NH) during the pre-industrial era. Methods We performed time-series analysis to examine the association between temperature change and country-wide/region-wide population collapses in different climatic zones. All of the known population collapse incidents in the NH in the period ce 800-1900 were included in our data analysis. Results Nearly 90% of population collapses in various NH countries/regions occurred during periods of climate deterioration characterized by shrinking carrying capacity of the land. In addition, we found that cooling dampened the human ecosystem and brought about 80% of the collapses in warmer humid, cooler humid and dry zones, while warming adversely affected the ecosystems in dry and tropical humid zones. All of the population collapses and growth declines in periods of warm climate occurred in dry and tropical humid zones. Malthusian checks (famines, wars and epidemics) were the dominant triggers of population collapses, which peaked dramatically when climate deteriorated. Main conclusions Global demographic catastrophes and most population collapse incidents occurred in periods with great climate change, owing to overpopulation caused by diminished carrying capacity of the land and the resultant outbreak of Malthusian checks. Impacts of cooling or warming on land carrying capacity varied geographically, as a result of the diversified ecosystems in different parts of the Earth. The observed climate-population synchrony challenges Malthusian theory and demonstrates that it is not population growth alone but climate-induced subsistence shortage and population growth working synergistically, that cause large-scale human population collapses on the long-term scale. © 2010 Blackwell Publishing Ltd.


Groenewold N.,University of Western Australia | Chen A.,Jinan University | Lee G.,Xi'an Jiaotong University
Regional Studies | Year: 2010

Groenewold N., Chen A. and Lee G. Inter-regional spillovers of policy shocks in China, Regional Studies. Inter-regional output spillovers from policy shocks are analysed in a three-region vector-autoregressive (VAR)-based model of China using investment as the policy variable. The contemporaneous effect of policy is greater in the coastal region than in the other two regions, and the effect in the central region is larger than in the western region, suggesting that at least part of the expenditure boosts in the poorer inland regions find their way to the coastal provinces. These results are confirmed when the effects of the policy shocks are simulated over time and are found to be generally robust to alternative model specifications. © 2010 Regional Studies Association.


Wang Y.,CAS Guangzhou Institute of Geochemistry | Wang Y.,University of Chinese Academy of Sciences | Luo C.,CAS Guangzhou Institute of Geochemistry | Li J.,CAS Guangzhou Institute of Geochemistry | And 3 more authors.
Environmental Pollution | Year: 2011

The concentration and composition of PBDEs in the soils and plants near a typical e-waste recycling site in South China were investigated. The total concentration of PBDEs (ΣPBDEs) in soil ranged from 4.8 to 533 ng/g dry wt. The ΣPBDEs in vegetation were from 2.1 to 217 ng/g dry wt. For the vegetable, the highest concentration of 19.9 ng/g dry wt. was observed in the shoot of Brassica alboglabra L. BDE 209 was the predominant congener in all samples. In comaprison with other e-waste contaminated sites in China, lower concentrations of PBDEs and higher concentrations of PCBs were observed in both soils and plants suggesting different e-waste types involved in the present study. The PBDEs contaminated vegetables around the e-waste dismantling site may pose a potential health risk to the local inhabitants. © 2010 Published by Elsevier Ltd.


Xu Y.,CNRS Chemistry Institute of Rennes | Xu Y.,Jinan University | Zhao L.,CNRS Chemistry Institute of Rennes | Li Y.,Jinan University | Doucet H.,CNRS Chemistry Institute of Rennes
Advanced Synthesis and Catalysis | Year: 2013

The PdCl(C3H5)(dppb)/KOAc system was found to be effective for the direct regioselective C-5 arylation of 3-acetylpyrroles with ortho-substituted aryl bromides. This procedure has been found to be tolerant to a variety of functional groups at C-2 of the aryl bromide such as methyl, formyl, nitrile, nitro, hydroxymethyl, chloro, fluoro or trifluoromethyl. The sequential direct C-5 arylation followed by C-2 arylation of such 3-substituted pyrroles allows the synthesis of 2,5-diaryl-3-acetylpyrroles in high yields. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Gao J.,Xi'an Jiaotong University | Xue J.-F.,Jinan University | Xu M.,Jinan University | Gui B.-S.,Xi'an Jiaotong University | And 2 more authors.
International journal of nanomedicine | Year: 2014

PURPOSE: This study aimed to accurately analyze the relationship between calcium oxalate (CaOx) stone formation and the components of urinary nanocrystallites.METHOD: High-resolution transmission electron microscopy (HRTEM), selected area electron diffraction, fast Fourier transformation of HRTEM, and energy dispersive X-ray spectroscopy were performed to analyze the components of these nanocrystallites.RESULTS: The main components of CaOx stones are calcium oxalate monohydrate and a small amount of dehydrate, while those of urinary nanocrystallites are calcium oxalate monohydrate, uric acid, and calcium phosphate. The mechanism of formation of CaOx stones was discussed based on the components of urinary nanocrystallites.CONCLUSION: The formation of CaOx stones is closely related both to the properties of urinary nanocrystallites and to the urinary components. The combination of HRTEM, fast Fourier transformation, selected area electron diffraction, and energy dispersive X-ray spectroscopy could be accurately performed to analyze the components of single urinary nanocrystallites. This result provides evidence for nanouric acid and/or nanocalcium phosphate crystallites as the central nidus to induce CaOx stone formation.


Du W.S.,Wuhan University | Hu B.Q.,Wuhan University | Zhao Y.,Jinan University
Information Sciences | Year: 2013

The connections between special types of fuzzy relations and properties of fuzzy rough approximation operators have been established in recent years, but ⊤-Euclidean fuzzy relation has not been considered yet. In the present paper, the necessary and sufficient conditions for a fuzzy relation being ⊤-Euclidean are investigated in three different fuzzy rough approximation spaces. Meanwhile, it is proved that in (I, ⊤)-fuzzy rough approximation space, where I is an R-implication, the properties the ⊤-Euclidean (I, ⊤)-fuzzy rough approximation operators possess are just the same as those in rough fuzzy approximation space. © 2012 Elsevier Inc. All rights reserved.


An T.,CAS Guangzhou Institute of Geochemistry | Yang H.,CAS Guangzhou Institute of Geochemistry | Yang H.,University of Chinese Academy of Sciences | Li G.,CAS Guangzhou Institute of Geochemistry | And 3 more authors.
Applied Catalysis B: Environmental | Year: 2010

Fluoroquinolones and their metabolites are found in surface and ground waters, indicating their ineffective removal by conventional water treatment technologies. Advanced oxidation processes (AOPs) are alternatives to traditional water treatments. They utilize free radical reactions to directly degrade fluoroquinolones. This work reports absolute rate constants for the reaction of ciprofloxacin with several free radicals, {radical dot}OH, {radical dot}N3 and SO4{radical dot}- as well as hydrated electrons. Pulsed radiolysis experiments showed that {radical dot}OH, {radical dot}N3 and eaq - reacted quickly with ciprofloxacin, with bimolecular reaction rate constants of (2.15 ± 0.10) × 1010, (2.90 ± 0.12) × 1010 and (2.65 ± 0.15) × 1010 M-1 s-1, respectively, while the SO4{radical dot}- radical appeared not to react with ciprofloxacin. Transient spectra were observed for the intermediate radicals produced by hydroxyl and azide radical reactions. Moreover, ciprofloxacin can be degraded rapidly using a typical advanced oxidation process, TiO2 photocatalysis, with half-lives of 1.9-10.9 min depending upon pH values. Seven degradation products were elucidated by LC/MS/MS analysis, and the degradation mechanism of ciprofloxacin was also tentatively proposed by combining the experimental evidence with theoretical calculations of frontier electron densities. The calculations suggest that the addition of a hydroxyl radical to ciprofloxacin and photo-hole direct attack is two predominant reaction pathways. © 2009 Elsevier B.V. All rights reserved.


Duan C.-Y.,Xi'an Jiaotong University | Xia Z.-Y.,Jinan University | Zhang G.-N.,Jinan University | Gui B.-S.,Xi'an Jiaotong University | And 2 more authors.
International Journal of Nanomedicine | Year: 2013

The property changes of urinary nanocrystallites in 13 patients with calcium oxalate (CaOx) stones were studied before and after ingestion of potassium citrate (K3cit), a therapeutic drug for stones. The analytical techniques included nanoparticle size analysis, transmission electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The studied properties included the components, morphologies, zeta potentials, particle size distributions, light intensity autocorrelation curves, and polydispersity indices (PDIs) of the nanocrystallites. The main components of the urinary nanocrystallites before K3cit intake included uric acid, β-calcium phosphate, and calcium oxalate monohydrate. After K3cit intake, the quantities, species, and percentages of aggregated crystals decreased, whereas the percentages of monosodium urate and calcium oxalate dehydrate increased, and some crystallites became blunt. Moreover, the urinary pH increased from 5.96 ± 0.43 to 6.46 ± 0.50, the crystallite size decreased from 524 ± 320 nm to 354 ± 173 nm, and the zeta potential decreased from -4.85 ± 2.87 mV to -8.77 ± 3.03 mV. The autocorrelation curves became smooth, the decay time decreased from 11.4 ± 3.2 ms to 4.3 ± 1.7 ms, and the PDI decreased from 0.67 ± 0.14 to 0.53 ± 0.19. These changes helped inhibit CaOx calculus formation. © 2013 Duan et al, publisher and licensee Dove Medical Press Ltd.


Yu L.,China Three Gorges University | Yu L.,Jinan University | Yin T.,Wuhan University
Journal of Vibration and Acoustics, Transactions of the ASME | Year: 2010

This paper proposed a practical damage detection method for frame structures based on finite element model-updating techniques. An objective function is defined as minimizing the discrepancies between the experimental and analytical modal parameters (namely, natural frequencies and mode shapes), which is set as a nonlinear least-squares problem with bound constraints. Unlike the commonly used line-search methods, the trust-region approach, a simple yet very powerful concept for minimization, is employed in order to make the optimization process more robust and reliable. Noting the objective function may sometimes be underdetermined for complex structures due to a relatively larger number of potential damaged elements, this paper attempts to propose a simple and convenient solution by expanding the original objective function. Moreover, the relative weighting scheme between different parts in the objective function is also investigated. One numerical two-story portal frame structure and two laboratory-tested frame structures, including a simple three-story steel frame structure and a more complex frame structure with bolted joints, are all adopted to evaluate the efficiency of the proposed technique. Some important issues about the application of the proposed method are also discussed in this paper. Copyright © 2010 by ASME.


Yang S.-L.,Huazhong University of Science and Technology | Liu L.-P.,Jinan University | Jiang J.-X.,Affiliated Hospital of Guiyang Medical College | Xiong Z.-F.,Huazhong University of Science and Technology | And 2 more authors.
Japanese Journal of Clinical Oncology | Year: 2014

Objectives: The roles of hypoxia-inducible factor-1α and hypoxia-inducible factor-2α in the development of hepatocellular carcinoma have not been fully elucidated. Here, we aim to uncover the relationship between the prognosis of hepatocellular carcinoma patients and the expression of hypoxia-inducible factor-1α and hypoxia-inducible factor-2α in tumor tissues. Methods: The protein levels of hypoxia-inducible factor-1α and hypoxia-inducible factor-2α were detected by immunohistochemistry on paraffin sections of 126 paired hepatocellular carcinoma tissue and peritumoral tissue samples. The mRNA levels of them were detected by quantitative real-time polymerase chain reaction. Results: High expression of hypoxia-inducible factor-1α was found in 57.1% (72/126) of tumor specimens, compared with 5.6% (7/126) in peritumoral tissues, while high expression of hypoxia-inducible factor-2α was found in only 13.5% (17/126) of tumors, compared with 47.6% (60/126) of peritumoral tissues. There was high expression of hypoxia-inducible factor-1α protein in hepatocellular carcinoma tissues closely associated with capsular infiltration and portal vein invasion, and thus lower overall survival and disease-free survival of hepatocellular carcinoma patients (P< 0.05). No significant association has been found between the expression of hypoxia-inducible factor-2α protein and capsular infiltration, portal vein invasion, overall survival and disease-free survival (P> 0.05). However, patients with high expression of both hypoxia-inducible factor-1α and hypoxia-inducible factor-2α have a significantly worse outcome than patients with low expression of both hypoxia-inducible factor-1α and hypoxia-inducible factor-2α (P< 0.05). Conclusions: The discordant results on expression of hypoxia-inducible factor-1α and hypoxia-inducible factor-2α suggest that these two proteins are differentially regulated in vivo, thus reflecting distinctive protein expression and stabilization mechanisms. The association between hypoxia-inducible factor-1α expression and unfavorable outcome indicates the importance of using hypoxia-inducible factor-1α as a treatment target in hepatocellular carcinoma. © The Author 2013. Published by Oxford University Press. All rights reserved.


Chen J.-F.,Jinan University | Morozov E.V.,University of New South Wales
Composite Structures | Year: 2016

This paper is concerned with the development and validation of a consistency elasto-viscoplastic damage model that accounts for the strain rate-dependent plastic response and the progressive post-failure behaviour of composite materials. The model proposed is suitable for progressive failure analysis of composite materials and structures subjected to loadings at various strain rates. A strain rate-dependent yield criterion is adopted; whereas the standard Kuhn-Tucker conditions for plastic loading and unloading remain valid. Also, the plastic consistency condition for strain rate-dependent material is satisfied. The relevant strain-driven implicit numerical integration procedure for the proposed material model is developed. A tangent stiffness operator including the strain rate-dependent effects and consistent with the integration procedure developed is derived to ensure the quadratic convergence rate pertaining to the Newton-Raphson method in a finite element analysis. The implicit integration algorithm is implemented in the finite element code Abaqus through the user-defined material subroutine UMAT. The validation of the proposed model is performed on S2/8552 glass/epoxy off-axis block composite specimens subjected to compression at various strain rates. It is shown that the strain rate-dependent response of composite materials is well captured and the predicted results agree well with the experimental data reported in the literature. © 2016 Elsevier Ltd.


Xu S.-H.,Guangzhou University | Xu S.-H.,Jinan University | Huang J.-Z.,Jinan University | Xu M.-L.,Jinan University | And 5 more authors.
Journal of Pathology | Year: 2015

Amplification of the activated Cdc42-associated kinase 1 (ACK1) gene is frequent in gastric cancer (GC). However, little is known about the clinical roles and molecular mechanisms of ACK1 abnormalities in GC. Here, we found that the ACK1 protein level and ACK1 phosphorylation at Tyr 284 were frequently elevated in GC and associated with poor patient survival. Ectopic ACK1 expression in GC cells induced epithelial-mesenchymal transition (EMT) and promoted migration and invasion in vitro, and metastasis in vivo; the depletion of ACK1 induced the opposite effects. We utilized SILAC quantitative proteomics to discover that the level of the cell cycle-related protein ecdysoneless homologue (ECD) was markedly altered by ACK1. Overexpression of ECD promoted EMT, migration, and invasion in GC, similar to the effects of ACK1 overexpression. Silencing of ECD completely blocked the augmentation of ACK1 overexpression-induced EMT, migration, and invasion. Mechanistically, ACK1 phosphorylated AKT at Thr 308 and Ser 473 and activated the AKT pathway to up-regulate the transcription factor POU2F1, which directly bound to the promoter region of its novel target gene ECD and thus regulated ECD expression in GC cells. Furthermore, the phosphorylation levels of AKT at Thr 308 and Ser 473 and POU2F1 and ECD levels were positively associated with ACK1 levels in clinical GC specimens. Collectively, we have demonstrated that ACK1 promotes EMT, migration, and invasion by activating AKT-POU2F1-ECD signalling in GC cells. ACK1 may be employed as a new prognostic factor and therapeutic target for GC. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.


Xu J.-C.,Key Laboratory of Disaster Forecast and Control in Engineering | Xu J.-C.,Jinan University | Zhang Y.,Jinan University
Gongcheng Lixue/Engineering Mechanics | Year: 2011

This paper performs a nonlinear dynamic stability analysis of truncated sandwich shallow spherical shells under the action of explosive impacts. Based on Reissner's hypothesis and Hamilton's principle, the nonlinear dynamic governing equation is obtained and solved by Galerkin method and Runge-Kutta method. Budiansky-Roth criterion expressed in terms of rigid center displacements is employed to determine the critical instability load. The effects of geometric parameters and mechanical parameters on impact critical instability load are discussed.


Zhang X.,State Key Laboratory of Optical Communications Technologies and Networks | Li Z.,Jinan University | Jiang T.,Huazhong University of Science and Technology | Li H.,State Key Laboratory of Optical Communications Technologies and Networks | And 7 more authors.
Optics Express | Year: 2013

In this paper, we experimentally demonstrate the first 110-Gb/s multi-band superchannel coherent optical orthogonal frequency-division multiplexing based on offset quadrature amplitude modulation (OFDM/OQAM) system. Unlike the conventional orthogonal band-multiplexed OFDM system, no timing or frequency synchronization is required for the OFDM/OQAM system. We further investigate the influence of guard band, and find that very trivial guard band spacing (<20MHz) is required without any sensitivity performance or spectral efficiency degradation. Thus, the newly designed scheme would significantly reduce the implementation constrains for the band-multiplexed superchannel coherent optical OFDM system. © 2013 Optical Society of America.


Zhang X.,Jinan University | Li Z.,Jinan University | Li C.,State Key Laboratory of Optical Communications Technologies and Networks | Li C.,Huazhong University of Science and Technology | And 4 more authors.
Optics Express | Year: 2014

In this paper, we propose a novel approach to simultaneously receive multi-band 100-Gb/s direct-detection optical signal with only one polarization and one conventional 40-GHz photodiode. The modulation format of orthogonal frequency-division multiplexing based on offset quadrature amplitude modulation (OFDM/OQAM) is selected to provide signal spectrum with high side-lobe suppression ratio, which can effectively reduce the electrical sub-band frequency interference. The whole 100-Gb/s OFDM/OQAM signal is comprised of 6 sub-bands with 16- and 32-QAM formats loading. Only one guard band is required to accommodate the overlapped 6-band signal-to-signal beat interference (SSBI). The receiver bandwidth is mainly limited by the digital storage oscilloscope (DSO) of 33 GHz. The transmission distance over standard single mode fiber (SSMF) is up to 320 km. © 2014 Optical Society of America.


Zhao T.,Southern Medical University | Li Y.,Southern Medical University | Wei W.,Meizhou peoples hospital | Savage S.,Imperial College London | And 2 more authors.
Neurobiology of Disease | Year: 2014

Commonly used anesthetic agents, e.g. ketamine, may be neurotoxic to the developing brain but there has been little attention to the neurobehavioral consequences for offspring when used for maternal anesthesia. We hypothesize that treatment of pregnant rats with ketamine during the second trimester would affect brain development of the offspring. Pregnant rats on gestational day 14, about equal to midtrimester pregnancy in humans, received a sedative dose of ketamine intravenously for 2. h. Brain hippocampal morphology of their pups at postnatal days 0 (P0) and P30 was examined by Nissl-staining and the characteristics of dendrites were determined using the Golgi-Cox staining, while cell proliferation in subventricular zone (SVZ) and dentate gyrus (DG) was labeled with bromodeoxyuridine (BrdU). Their neurobehavioral functions were tested at P25-30 after which the NR1 and NR2 subunits of N-methyl-d-aspartate (NMDA) receptor, brain-derived neurotrophic factor (BDNF) and postsynaptic density protein 95 (PSD-95) in the hippocampus were analyzed by western blot. When pregnant rats were exposed to ketamine, there was neuronal loss, pyramidal neuronal abnormality and reduced cell proliferation in the hippocampus of offspring. These morphological abnormalities were associated with depression- and anxiety-like behaviors, and impaired memory up to young adult age. The treatment further caused NR2A receptor subunit up-regulation and NR2B receptor subunit, BDNF and PSD-95 down-regulation. These data suggest that maternal anesthesia with ketamine during the fetal brain development period can cause fetal brain damage and subsequent neurobehavioral abnormality, which is likely associated with the imbalanced expression of NMDA receptor subunits. © 2014.


Xue J.,Jinan University | Yuan D.,Jinan University | Han F.,Beijing Institute of Technology | Liu R.,Jinan University
European Journal of Mechanics, A/Solids | Year: 2013

Conventionally cylindrical shells are often treated as shallow shells whose governing equation, according to Karman-Donnell's theory, can be approximated by those of thin plates. In this paper Karman-Donnell's theory for shallow shells is extended for long cylindrical shells undergoing large, nonlinear flexural deflection. The kinematic relations between the changes of curvature and the displacement are derived and the governing equations are established by considering the influence of the initial curvature of the cylindrical shells. In particular, the extended Karman-Donnell's theory is applied for the failure analysis of infinitely cylindrical shell under lateral pressure. A regional collapse mode is identified to occur in the shell with a longitudinal span proportional to (radius 3/thickness) (1/2) and a transverse profile of dog bone shape. It is found that the buckling pressure of the shell is in proportion to (thickness/radius) 3 and converges to the classic solution given by Timoshenko and Gere (1961). A comparison to the previous works indicates that ignoring the effect of the initial curvature will result in an overestimate of the buckling pressure for 33%. It shows that the initial curvature of long cylindrical shells has significant influence on the load carrying capacity and the extended Karman-Donnell's equations give very accurate predictions. © 2012 Elsevier Masson SAS. All rights reserved.


Ding D.-P.,Southern Medical University | He X.-F.,Southern Medical University | Zhang Y.,Jinan University
Breast Cancer Research and Treatment | Year: 2011

The xeroderma pigmentosum group G (XPG or ERCC5) and group F (XPF or ERCC4) play an important role in DNA repair, and produce dual incision 3 and 5 to the damaged nucleotide fragment. Several polymorphisms in the XPF and XPG gene have been described, including the commonly occurring Asp1104His in XPG and Arg415Gln in XPF. The published data on the association between these polymorphisms and breast cancer remained controversial. This meta-analysis of literatures was performed to derive a more precise estimation of the relationship. A total of 17 studies were identified to the meta-analysis, including 5,235 cases and 5,685 controls for XPG Asp1104His (from ten studies) and 3,910 cases and 3,985 controls for XPF Arg415Gln (from seven studies). Overall, no significantly elevated breast cancer risk was found in all genetic models when all studies were pooled into the meta-analysis (for XPG Asp1104His Asp/His vs. Asp/Asp: OR 1.02, 95% CI 0.94-1.11; His/His vs. Asp/Asp: OR 0.96, 95% CI 0.83-1.11; dominant model: OR 1.01, 95% CI 0.94-1.09; and for XPF Arg415Gln Arg/Gln vs. Arg/Arg: OR 1.00, 95% CI 0.89-1.12; Gln/Gln vs. Arg/Arg: OR 2.40, 95% CI 0.62-9.22; dominant model: OR 1.03, 95% CI 0.90-1.18). In stratified analyses, we observed an overall OR of 5.20 (95% CI 2.08-12.95) for breast cancer developing risk in the Caucasian ethnicity, comparing Gln/Gln type to wild-type Arg/Arg for Arg415Gln polymorphism. In conclusion, this meta-analysis suggests that XPG Asp1104His polymorphism is not associated with increased breast cancer risk, and XPF Arg415Gln may be a low-penetrant risk factor in the Caucasian ethnicity for developing breast cancer. © 2011 Springer Science+Business Media, LLC.


Xu Y.,Guangzhou University | Xu Y.,Jinan University | Miroshnichenko A.E.,Australian National University
Physical Review B - Condensed Matter and Materials Physics | Year: 2014

We propose a dynamically tunable nonreciprocal response for wave propagations by employing nonlinear Fano resonances. We demonstrate that the transmission contrast between waves propagating in opposite directions can be controlled by an excitation signal. In particular, the unidirectional transmission can be flipped at different time of a pulse, resembling a Fano diode with dynamical reconfigurable nonreciprocity. The key mechanism is the interaction between a linear and a nonlinear Fano resonance that allows for the tunable unidirectional wave propagation and ultrahigh transmission contrast ratio. We further present a realistic photonic example which demonstrates the properties of nonreciprocity can be dynamically manipulated using a pump pulse, based on the general theoretical model. © 2014 American Physical Society.


Yuan T.-F.,Nanjing Normal University | Yuan T.-F.,University of Hong Kong | Liang Y.-X.,University of Hong Kong | So K.-F.,University of Hong Kong | So K.-F.,Jinan University
Frontiers in Neuroanatomy | Year: 2015

Adult neurogenesis has been well studied in hippocampus and subventricular zone (SVZ); while this is much less appreciated in other brain regions, including amygdala, hypothalamus, and piriform cortex (PC). The present review aims at summarizing recent advances on the occurrence of new neurons in the PC, their potential origin, and migration route from the SVZ. We further discuss the relevant implications in olfactory dysfunction accompanying the neurodegenerative diseases. © 2015 Yuan, Liang and So.


Liu T.,Southern Medical University | Xue W.,Jinan University | Ke B.,Jiangxi Provincial Peoples Hospital | Xie M.-Q.,Southern Medical University | Ma D.,Jinan University
Biomaterials | Year: 2014

A new cyclodextrin derivative (CD-PLLD) consisting of a β-cyclodextrin core and poly(l-lysine) dendron arms was prepared by the click conjugation of per-6-azido-b-cyclodextrin with propargyl focal point poly(l-lysine) dendron of third generation, and then used for docetaxel (DOC) and the best siRNA plasmid targeting MMP-9 (pMR3) co-delivery. Different from commonly used amphiphilic copolymers with cationic character, the as obtained cyclodextrin derivative may be used directly for the combinatorial delivery of nucleic acid and lipophilic anticancer drugs without a complicated micellization process. It was found that CD-PLLD/pMR3 nanocomplex showed a good gene transfection efficiency invitro, and could mediate the reduce of MMP-9 protein in HNE-1 cells. For co-delivery analysis, the obtained CD-PLLD/DOC/pMR3 complexes could induce a more significant apoptosis than DOC or pMR3 used only, and decreased invasive capacity of HNE-1 cells. Moreover, the star-shaped copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k in the hemolysis and MTT assays, which should be encouraged in nasopharyngeal cancer therapy. © 2014 Elsevier Ltd.


Gao Q.,Max Planck Institute of Colloids and Interfaces | Gao Q.,Jinan University | Giordano C.,Max Planck Institute of Colloids and Interfaces | Antonietti M.,Max Planck Institute of Colloids and Interfaces
Angewandte Chemie - International Edition | Year: 2012

Learning from nature: The development of an MoS2/Ta 3N5 nanocomposite as a catalyst for selective aerobic oxidation by O2 activation was inspired by the nitrogenase enzymes in nature. The superior performance of this biomimetic catalyst, which shows potential for the selective oxidation of multifunctional substrates (see picture), results from the integration of Ta3N5 and MoS2 at the nanoscale and the synergistic enhancement of their activity. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Deng Z.-H.,Shenzhen Blood Center | Zhen J.-X.,Shenzhen Blood Center | Xu Y.-P.,Shenzhen Blood Center | Zhao J.,Jinan University
Tissue Antigens | Year: 2014

The novel HLA-DPB1*166:01 allele differs from the closest allele DPB1*152:01 by a single missense mutation at CDS nt 315G>A in exon 2. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


Yang J.,Southern Medical University | Li M.,Jinan University | Shen X.,Southern Medical University | Liu S.,Southern Medical University
Viruses | Year: 2012

Influenza A virus (IAV) has caused seasonal influenza epidemics and influenza pandemics, which resulted in serious threat to public health and socioeconomic impacts. Until now, only 5 drugs belong to two categories are used for prophylaxis and treatment of IAV infection. Hemagglutinin (HA), the envelope glycoprotein of IAV, plays a critical role in viral binding, fusion and entry. Therefore, HA is an attractive target for developing anti-IAV drugs to block the entry step of IAV infection. Here we reviewed the recent progress in the study of conformational changes of HA during viral fusion process and the development of HA-based IAV entry inhibitors, which may provide a new choice for controlling future influenza pandemics. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Ouyang D.-Y.,Jinan University | Xu L.-H.,Jinan University | He X.-H.,Jinan University | Zhang Y.-T.,Jinan University | And 3 more authors.
Autophagy | Year: 2013

Autophagic responses to chemotherapeutic agents may vary greatly among different prostate cancer cells and have not been well characterized. In this study, we showed that valproic acid (VPA) induced conversion of LC3-I to LC3-II and formation of LC3 puncta, the typical markers of autophagy, in LNCaP and PC-3 cells. However, these markers were undetectable in DU145 cells upon autophagic stimulation, indicating a defect of autophagy in this cell line. Among several critical autophagy-related proteins, ATG5 and ATG12-ATG5 conjugates, which are essential for autophagy induction, were absent in DU145 cells. No canonical transcripts for full-length ATG5 but only two alternatively spliced ATG5 transcripts were identified in DU145 cells. These alternative transcripts lack one or two exons, leading to premature termination of ATG5 translation. Transfection of the wild-type ATG5 gene into DU145 cells rescued the production of ATG5 and ATG12-ATG5 conjugates, resulting in formation of LC3-II conjugates and LC3 puncta. Moreover, the levels of the SQSTM1 protein, which should be degradable as an autophagy adaptor, were much higher in DU145 than in LNCaP and PC-3 cells, but were significantly decreased after ATG5 restoration in DU145 cells. However, expression of wild-type ATG5 in DU145 or knockdown of ATG5 in LNCaP and PC-3 cells did not change the inhibitory effects of VPA on these cells. Collectively, these results indicated that VPA-induced autophagy in prostate cancer cells depended on ATG5 and more importantly, that the autophagy pathway was genetically impaired in DU145 cells, suggesting caution in interpreting autophagic responses in this cell line. © 2013 Landes Bioscience.


Zhao C.,Jinan University | Zhao C.,Guangzhou University | Dai C.,Jinan University | Chen X.,Jinan University
International Journal of Hyperthermia | Year: 2012

Background: Currently known as a metastatic disease, stage IV gastric cancer cannot be cured by surgery, but treatments are recommended to relieve symptoms such as pain and to prolong survival. Methods: With better access to metastases and certain large or inoperable tumours, we applied two treatment sessions of combined therapy of whole-body hyperthermia and hyperthermic intraperitoneal chemo-perfusion in the treatment group, while patients in the control group were treated with oxaliplatin combined with 5-fluorouracil chemotherapy or Xeloda. We used the RECIST criteria for outcome evaluation. Results: With the combined treatment, we found the complete and partial remission rate of patients to be 61.5%, and the rate of stable disease was 19.2%. Symptoms such as pain and a large volume of ascites were alleviated, and the quality of life was correspondingly improved. In addition, the combined treatment had a significant therapeutic benefit against the primary tumour and the metastases to the lymph nodes and liver. Survival time was also significantly prolonged (the 1-year survival rate was 38.5% compared to the control group rate of 19%). Conclusions: These results suggest that whole-body hyperthermia combined with hyperthermic intraperitoneal chemotherapy is an effective treatment for patients with advanced gastric malignancies. © 2012 Informa UK Ltd.


Cheng K.-W.,University of Hong Kong | Wong C.-C.,University of Hong Kong | Wang M.,University of Hong Kong | He Q.-Y.,Jinan University | Chen F.,University of Hong Kong
Mass Spectrometry Reviews | Year: 2010

Natural products, and their derivatives and mimics, have contributed to the development of important therapeutics to combat diseases such as infections and cancers over the past decades. The value of natural products to modern drug discovery is still considerable. However, its development is hampered by a lack of a mechanistic understanding of their molecular action, as opposed to the emerging molecule-targeted therapeutics that are tailored to a specific protein target(s). Recent advances in the mass spectrometry-based proteomic approaches have the potential to offer unprecedented insights into the molecular action of natural products. Chemical proteomics is established as an invaluable tool for the identification of protein targets of natural products. Small-molecule affinity selection combined with mass spectrometry is a successful strategy to "fish"cellular targets from the entire proteome. Mass spectrometry-based profiling of protein expression is also routinely employed to elucidate molecular pathways involved in the therapeutic and possible toxicological responses upon treatment with natural products. In addition, mass spectrometry is increasingly utilized to probe structural aspects of natural products-protein interactions. Limited proteolysis, photoaffinity labeling, and hydrogen/deuterium exchange in conjunction with mass spectrometry are sensitive and high-throughput strategies that provide low-resolution structural information of non-covalent natural product-protein complexes. In this review, we provide an overview on the applications of mass spectrometry-based techniques in the identification and characterization of natural product-protein interactions, and we describe how these applications might revolutionize natural product-based drug discovery. © 2009 Wiley Periodicals, Inc.


Jiao H.-P.,Jinan University | Yu X.,Jinan University | Liu Z.-Q.,Guangzhou University | Kuang P.-Y.,Guangzhou University | Zhang Y.-M.,Jinan University
RSC Advances | Year: 2015

To overcome the drawback of low photocatalytic efficiency brought by fast electron-hole recombination and narrow photoresponse range, the heterostructured Bi2S3/BiOBr microspheres were designed and synthesized via a facile one-pot solvothermal method. The as-prepared heterostructured Bi2S3/BiOBr photocatalyst exhibits significantly enhanced photoelectrochemical performance and photocatalytic activity for decomposing rhodamine B (RhB) and Brilliant ponceau 5R (BP) compared with pure BiOBr under visible light irradiation, which could be mainly attributed to the formation of a heterojunction in the Bi2S3/BiOBr interface, which can efficiently facilitate the separation of photogenerated electron-hole pairs. Furthermore, a terephthalic acid photoluminescence (TA-PL) probing test and radical trapping experiments demonstrate that the h+ and ·O2- radicals are the dominant reactive species while·OH radicals could be neglected. In addition, the possible enhanced photocatalytic mechanism is proposed on the basis of the calculated energy band positions and radical trapping experiments. © 2015 The Royal Society of Chemistry.


Gao W.,University of Hong Kong | Li H.-Y.,University of Hong Kong | Li H.-Y.,Jinan University | Xiao S.,University of Hong Kong | Chye M.-L.,University of Hong Kong
Plant Journal | Year: 2010

Summary Lysophospholipids are intermediates of phospholipid metabolism resulting from stress and lysophospholipases detoxify lysophosphatidylcholine (lysoPC). Many lysophospholipases have been characterized in mammals and bacteria, but few have been reported from plants. Arabidopsis thaliana lysophospholipase 2 (lysoPL2) (At1g52760) was identified as a protein interactor of acyl-CoA-binding protein 2 (ACBP2) in yeast two-hybrid analysis and co-immunoprecipitation assays. BLASTP analysis indicated that lysoPL2 showed ∼35% amino acid identity to the lysoPL1 family. Co-localization of autofluorescence-tagged lysoPL2 and ACBP2 by confocal microscopy in agroinfiltrated tobacco suggests the plasma membrane as a site for their subcellular interaction. LysoPL2 mRNA was induced by zinc (Zn) and hydrogen peroxide (H2O2), and lysoPL2 knockout mutants showed enhanced sensitivity to Zn and H2O2 in comparison to wild type. LysoPL2-overexpressing Arabidopsis was more tolerant to H 2O2 and cadmium (Cd) than wild type, suggesting involvement of lysoPL2 in phospholipid repair following lipid peroxidation arising from metal-induced stress. Lipid hydroperoxide (LOOH) contents in ACBP2-overexpressors and lysoPL2-overexpressors after Cd-treatment were lower than wild type, indicating that ACBP2 and lysoPL2 confer protection during oxidative stress. A role for lysoPL2 in lysoPC detoxification was demonstrated when recombinant lysoPL2 was observed to degrade lysoPC in vitro. Filter-binding assays and Lipidex competition assays showed that (His)6-ACBP2 binds lysoPC in vitro. Binding was disrupted in a (His)6-ACBP2 derivative lacking the acyl-CoA-binding domain, confirming that this domain confers lysoPC binding. These results suggest that ACBP2 can bind both lysoPC and lysoPL2 to promote the degradation of lysoPC in response to Cd-induced oxidative stress. © 2010 Blackwell Publishing Ltd.


He L.,Jinan University | Chen T.,Jinan University | You Y.,Jinan University | Hu H.,Jinan University | And 5 more authors.
Angewandte Chemie - International Edition | Year: 2014

Construction of delivery systems for anticancer gold complexes to decrease their toxicity while maintaining efficacy is a key strategy to optimize and develop anticancer gold medicines. Herein, we describe cancer-targeted mesoporous silica nanoparticles (MSN) for delivery of a gold(III) porphyrin complex (Au-1a@MSN(R)) to enhance its anticancer efficacy and selectivity between cancer and normal cells. Encapsulation of Au-1a within mesoporous silica nanoparticles amplifies its inhibitory effects on thioredoxin reductase (TrxR), resulting in a loss of redox balance and overproduction of reactive oxygen species (ROS). Elevated cellular oxidative stress activates diversified downstream ROS-mediated signaling pathways, leading to enhanced apoptosis-inducing efficacy. © 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.


Yang L.,Leibniz Institute for Solid State and Materials Research | Yang L.,South China University of Technology | Wang S.,Fudan University | Mao J.,Fudan University | And 6 more authors.
Advanced Materials | Year: 2013

Hierarchical MoS2/polyaniline nanowires, integrating MoS 2 nanosheets with conductive polyaniline, serve as prominent anode materials for Li-ion batteries, presenting high capacity and good cyclability. The polyaniline-hybrid structure and hierarchical features significantly promote the Li-storage performance as compared with the bare MoS2, indicating new opportunities for developing electrode nanomaterials. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Yu M.,Jinan University | Ren Q.,Guangzhou University | Yu S.Y.,Guangzhou University
Molecular Biology Reports | Year: 2014

The phosphorylation of nephrin plays an important role in maintaining the normal structure and function in podocytes. Dexamethasone (Dex) is usually used to treat glomerular diseases with proteinuria. In this study, we observated the effect of Dex and angiotensin II (AngII) on the change of nephrin phosphorylation in cultured podocytes. In vitro, cultured podocytes were exposed to AngII (10-6 mol/L) pretreated with or without Dex (100 nM) for different time periods. Nck or Fyn were silenced by small interfering RNA (siRNA), nephrin and its phosphorylation expression were analyzed by Western blotting. In vitro, the phosphorylation of nephrin was significantly reduced after AngII stimulation (P < 0.05). Dex significantly resisted podocyte injury inducted by AngII via increasing the phosphorylation of nephrin (P < 0.05), siRNA silencing Nck can partially inhibited nephrin phosphorylation, siRNA silencing Fyn can completely inhibited nephrin phosphorylation. Phosphorylation of nephrin is important for the survival status of podocytes. Glucocorticoid treatment for human glomerulonephritis may exert its function by regulating Nck and Fyn complex to promote phosphorylation of nephrin. These results elucidate a novel mechanism of glucocorticoid treatment for glomerulonephritis. © 2014 Springer Science+Business Media.


Lian Y.,Jinan University | Xie L.,Jinan University | Chen M.,Southern Medical University | Chen L.,Jinan University
Evidence-based Complementary and Alternative Medicine | Year: 2014

Objective. To investigate the effects and to analyze the mechanism of the combination of Astragalus polysaccharide (APS) and Rhein on apoptosis in rats with chronic renal failure (CRF). Methods. Thirty-seven male Wistar rats were randomly divided into a control group, a model group, a low-dose APS and Rhein combination group, and a high-dose APS and Rhein combination group. CRF was induced by orogastric gavage with adenine. Rats were observed for renal function, electrolyte, and pathological changes for 7 weeks after administration. Renal tubular cell apoptosis was assessed by TUNEL and protein expressions of IRE1 and CHOP were detected by Western-blotting. Results. The combination of APS and Rhein decreased the kidney weight and index, improved renal pathological injury, maintained the stability of serum electrolytes, and reduced SCr and BUN levels in rat models. Moreover, APS and Rhein combination could effectively inhibit the apoptosis and reduce the protein expressions of IRE1and CHOP of renal tubular cells. Conclusions. The combination of APS and Rhein could improve renal function and reduce renal cell apoptosis to protect against further progression of CRF, whose mechanism may be related to alleviate endoplasmic reticulum stress (ERS) in the renal cells. © 2014 Yonghong Lian et al.


Chen H.Y.,Jinan University | Tse T.H.,University of Hong Kong
IEEE Transactions on Software Engineering | Year: 2013

Algebraic specifications have been used in the testing of object-oriented programs and received much attention since the 1990s. It is generally believed that class-level testing based on algebraic specifications involves two independent aspects: the testing of equivalent and nonequivalent ground terms. Researchers have cited intuitive examples to illustrate the philosophy that even if an implementation satisfies all the requirements specified by the equivalence of ground terms, it may still fail to satisfy some of the requirements specified by the nonequivalence of ground terms. Thus, both the testing of equivalent ground terms and the testing of nonequivalent ground terms have been considered as significant and cannot replace each other. In this paper, we present an innovative finding that, given any canonical specification of a class with proper imports, a complete implementation satisfies all the observationally equivalent ground terms if and only if it satisfies all the observationally nonequivalent ground terms. As a result, these two aspects of software testing cover each other and can therefore replace each other. These findings provide a deeper understanding of software testing based on algebraic specifications, rendering the theory more elegant and complete. We also highlight a couple of important practical implications of our theoretical results. © 1976-2012 IEEE.


Trandem K.,University of Iowa | Anghelina D.,University of Iowa | Zhao J.,University of Iowa | Zhao J.,Jinan University | Perlman S.,University of Iowa
Journal of Immunology | Year: 2010

Mice infected with the neurotropic JHM strain of mouse hepatitis virus (JHMV) develop acute and chronic demyelinating diseases with histopathological similarities to multiple sclerosis. The process of demyelination is largely immune-mediated, as immunodeficient mice (RAG1-/- mice) do not develop demyelination upon infection; however, demyelination develops if these mice are reconstituted with either JHMV-immune CD4 or CD8 T cells. Because myelin destruction is a consequence of the inflammatory response associated with virus clearance, we reasoned that decreasing the amount of inflammation would diminish clinical disease and demyelination. Given that regulatory T cells (Tregs) have potent anti-inflammatory effects, we adoptively transferred Tregs into infected C57BL/6 and RAG1-/- mice. In both instances, transfer of Tregs decreased weight loss, clinical scores, and demyelination. Transferred Tregs were not detected in the CNS of infected RAG1-/- mice, but rather appeared to mediate their effects in the draining cervical lymph nodes. We show that Tregs dampen the inflammatory response mediated by transferred JHMV-immune splenocytes in infected RAG1-/- mice by decreasing T cell proliferation, dendritic cell activation, and proinflammatory cytokine/chemokine production, without inducing apoptosis. By extension, decreasing inflammation, whether by Treg transfer or by otherwise enhancing the anti-inflammatory milieu, could contribute to improved clinical outcomes in patients with virus-induced demyelination. Copyright © 2010 by The American Association of Immunologists, Inc.


Zhao J.,University of Iowa | Zhao J.,Jinan University | Legge K.,University of Iowa | Perlman S.,University of Iowa
Journal of Clinical Investigation | Year: 2011

The morbidity and mortality associated with respiratory virus infection is felt most keenly among the elderly. T cells are necessary for viral clearance, and many age-dependent intrinsic T cell defects have been documented. However, the development of robust T cell responses in the lung also requires respiratory DCs (rDCs), which must process antigen and migrate to draining LNs (DLNs), and little is known about age-related defects in these T cell-extrinsic functions. Here, we show that increases in prostaglandin D 2 (PGD 2) expression in mouse lungs upon aging correlate with a progressive impairment in rDC migration to DLNs. Decreased rDC migration resulted in diminished T cell responses and more severe clinical disease in older mice infected with respiratory viruses. Diminished rDC migration associated with virus-specific defects in T cell responses and was not a result of cell-intrinsic defect, rather it reflected the observed age-dependent increases in PGD 2 expression. Blocking PGD 2 function with small-molecule antagonists enhanced rDC migration, T cell responses, and survival. This effect correlated with upregulation on rDCs of CCR7, a chemokine receptor involved in DC chemotaxis. Our results suggest that inhibiting PGD 2 function may be a useful approach to enhance T cell responses against respiratory viruses in older humans.


Lin J.,Southern Medical University | Lin J.,Jinan University | Lin J.,Hunan University
Biochimica et biophysica acta | Year: 2015

BACKGROUND: Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work.METHODS: Specifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen-antibody reaction was studied by using human red blood cell agglutination assay.RESULTS: The results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen-antibody reaction.CONCLUSIONS: From these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups.GENERAL SIGNIFICANCE: This work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers. Copyright © 2014 Elsevier B.V. All rights reserved.


Pan B.,Jinan University | Zheng S.,Southern Medical University | Liu C.,Southern Medical University | Xu Y.,Southern Medical University
Molecular Biology Reports | Year: 2013

To investigate the immunoglobulin G (IgG) expression in prostate cancer cell lines and explore the effects of IGHG1 gene knockdown on PC3 cell growth and apoptosis. Flow cytometry, qPCR and western blot were used to demonstrate IgG expression in prostate cancer cell lines. PC3 cells were transfected with designed siRNA, the expression of IgG was determined by qPCR and western blot, the proliferation and apoptosis were detected by 3-(4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxy-phenyl)-2-(4-sulfophenil)-2H-tetrazolium, inner salt (MTS) and flow cytometry. The percentages of IgG in LNCaP cell membrane and cytoplasm were 2.96 and 89.22% by flow cytometer, those of PC3 cell were 86.73 and 90.99% respectively. The average level of IgG1 mRNA expression in PC3 cell line was significantly higher than that in LNCaP cell line (3.08±0.15 vs 1.00±0.37, P=0.001). The protein level of IgG expression of PC3 cell line was 1.92±0.15, compared with LNCaP cell line (1.05±0.86). The expression of IgG1 mRNA and protein level in transfected PC3 cells decreased, with significant statistical differences from the blank control group (P<0.01). The PC3 cell growth inhibition rates were 31.3 and 43.3% in 48 and 72h respectively. The rate of apoptotic PC3 cells were 5.29±0.41% in experimental group higher than that in control group (1.49±0.29%) (P<0.01). IgG was identified in prostate cancer cells, and the siRNA targeted silencing of IGHG1 can inhibit cell viability and promote apoptosis, which might therefore act as a potential target in prostate cancer gene therapy. © 2012 Springer Science+Business Media Dordrecht.


Gao F.,Jinan University | Ye Y.,Wannan Medical College | Zhang Y.,Southern Medical University | Yang J.,Jinan University
Clinical Biochemistry | Year: 2013

Objectives: Researchers have proposed that cancer stem cells (CSCs) are the driving force of tumorigenesis, recurrence, and metastases, contributing to the failure of some cancer treatments. Water bath hyperthermia, a novel treatment for cancer, can be highly effective when combined with chemotherapy, radiotherapy, or immunotherapy. Side population (SP) cells are part of a subpopulation of cells that have stem cell-like characteristics. In preliminary experiments, we discovered a decrease in SP cells in heat-treated colon cancer cells, which encouraged us to investigate whether hyperthermia had in fact played a role in regulating the stemness of colon cancer cells. Design and methods: We determined the number of SP cells and CD133-positive cells using flow cytometry; surveyed the expression of stemness-related genes and proteins by using quantitative reverse transcriptase-polymerase chain reaction and Western blot analysis, respectively; and evaluated tumor formation ability by using the tumor sphere formation assay. Results: We found that water bath hyperthermia at 40. °C results in a decreased percentage of SP cells (i.e. decreased from 0.6% to 0.0% and 1.1% to 0.1% among heat-treated HCT116 cells and SW620 cell respectively) and CD133-positive cells (i.e. decreased from 25.1. ±. 1.2% to 18.7. ±. 1.3% and 33.6. ±. 3.2% to 23.5. ±. 2.5% among heat-treated HCT116 cells and SW620 cell separately), weakens stem cell self-renewal properties (i.e., tumor colony formation ability), and downregulates the expression of stem-like cellular markers in colon cancer cells. Conclusions: All the findings indicate that hyperthermia has an important effect on the stemness of colon cancer cells. © 2013 The Canadian Society of Clinical Chemists.