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Du Y.,Jinan Stomatological Hospital
Journal of investigative and clinical dentistry | Year: 2013

A case of endodontic treatment of a mandibular first premolar exhibiting a total of four distinct root canals and four apical foramina is described. This occurrence in mandibular first premolar has rarely been reported in the endodontic literature. Endodontic treatment that considers the anatomic variation of root canal morphology is important to ensure a favorable healing outcome, and its identification could be enhanced by careful examination using a dental operating microscope. Obturation of root canals using a warm vertical compaction technique with a highly-radiopaque root canal sealer, such as AH Plus, after careful ultrasonic activated irrigation with ethylenediaminetetraacetic acid might allow the flow of sealer into the narrowed but unprepared part of the canal. This offers valuable adjuncts for the successful negotiation of calcified main canals, thereby facilitating optimum chemo-mechanical debridement of the root canal system. © 2013 Blackwell Publishing Asia Pty Ltd.

Ji X.,Shandong University | Zhang L.,Jinan Stomatological Hospital | Peng J.,Shandong University | Hou M.,Shandong University
Journal of Hematology and Oncology | Year: 2014

Immune thrombocytopenia is an autoimmune disease with abnormal T cell immunity. Cytotoxic T cells, abnormal T regulatory cells, helper T cell imbalance, megakaryocyte maturation abnormalities and abnormal T cell anergy are involved in the pathogenesis of this condition. The loss of T cell-mediated immune tolerance to platelet auto-antigens plays a crucial role in immune thrombocytopenia. The induction of T cell tolerance is an important mechanism by which the pathogenesis and treatment of immune thrombocytopenia can be studied. Studies regarding the roles of the new inducible costimulator signal transduction pathway, the ubiquitin proteasome pathway, and the nuclear factor kappa B signal transduction pathway in the induction of T cell tolerance can help improve our understanding of immune theory and may provide a new theoretical basis for studying the pathogenesis and treatment of immune thrombocytopenia. © 2014 Ji et al.; licensee BioMed Central Ltd.

Wu G.,Jinan General Military Hospital | Chen L.,Jinan Stomatological Hospital | Zhu G.,Jinan General Military Hospital | Wang Y.,Jinan General Military Hospital
Journal of Surgical Research | Year: 2013

Background: To investigate whether low-intensity ultrasound accelerates healing in bone tissues close to dental implants with osteoradionecrosis (ORN) of the mandible and is suitable for development as a therapy in patients with dental implants receiving radiotherapy. Materials and methods: Dog models of radiative bone injury surrounding dental implants in both sides of mandible were established by four treatment methods of radiotherapy, each 15 Gy. After radiative treatment, antibiotics were administered and the left injury was treated with ultrasound and the right with debridement. Measures for evaluation included spiral computed tomography (SCT), Micro-CT, microvessel density, and pull-out experiment, and data were collected and analyzed. Results: After 4 months of radiotherapy, both sides of mandible displayed preclinic symptom of radiative osteonecrosis. Microvessel density of the side treated by ultrasound was 6.2152 ± 0.6508 and that of the debridement side was 3.8490 ± 0.8954 (P < 0.05). Micro-CT results showed that bone volume fraction of trabecula, thickness of trabecula, trabecula spacing, ratio of bone surface area to bone volume, and trabecula number of the ultrasound-treated mandible were 0.3605 ± 0.0337, 0.0287 ± 0.0045, 0.0369 ± 0.0073, 71.6124 ± 14.1649, and 7.2915 ± 1.4937, whereas those of the debridement side were 0.1779 ± 0.0178, 0.0151 ± 0.0021, 0.6623 ± 0.1125, 33.2686 ± 5.949, and 5.0689 ± 0.5028, respectively; statistical significance was observed (P < 0.05). Pull-out experiment suggested that pull-out strength of the ultrasound-treated side was 0.5793 ± 0.1066 whereas that of the debridement side was 0.2980 ± 0.0243, representing a statistical significance (P < 0.05). Conclusions: Low-intensity ultrasound can accelerate the healing of bone tissues surrounding dental implants in osteoradionecrosis of the mandible animals.

Han Q.-Q.,Shandong University | Du Y.,Jinan Stomatological Hospital | Yang P.-S.,Shandong University
Future Medicinal Chemistry | Year: 2013

Although several methods have been used in bone regeneration medicine, current methods still have many limitations. The tissue used for autogenous bone graft is limited and allograft has weak osteoinductive activity. Tissue engineering provides a good choice for bone regeneration. However, the growth factors needed have a high price and short half-life. Recently, a number of small molecules have been confirmed to have osteoinductive activity and some have been clinically used. Natural small molecules including decalpenic acid, flavonoids, quinones can be extracted from plants and others can be synthesized according to the structure designed or mimicking the structure of natural small molecules. Small molecules can act as co-activator of BMP2 pathway or activate Wnt pathway; others can be the inhibitors of NF-κB signaling pathway. This review gives an overview on the small molecules with osteoinductive activity and discusses the mechanism of the small molecules. © 2013 Future Science Ltd.

Xu W.,Shandong University | Deng Z.,Shandong Academy of Sciences | Guo H.,Jinan Stomatological Hospital | Ling P.,Shandong University
Biomedical Chromatography | Year: 2012

A sensitive, accurate, rapid and robust LC-MS-MS method for the quantification of aucubin, a major bioactive constituent of Aucuba japonica, Eucommia ulmoides and Plantago asiatica, was established and validated in rat plasma. Plasma samples were simply precipitated by adding methanol and the supernatant was chromatographed by a Diamonsil® C18(2) column with the mobile phase comprising a mixture of 10mm ammonium acetate in methanol and that in water with the ratio of 50:50 (v/v). Quantification of aucubin was performed by mass spectrometry in the multiple-reaction monitoring mode with positive atmospheric ionization at m/z 364→149 for aucubin, and m/z 380→165 for catalpol (IS), respectively. The retention time was 2.47 and 2.44min for aucubin and the IS, respectively. The calibration curve (10.0-30,000ng/mL) was linear (r2>0.99) and the lower limit of quantification was 10.0ng/mL in the rat plasma sample. The method showed satisfactory results such as sensitivity, specificity, precision, accuracy, recovery, freeze-thaw and long-term stability. This simple LC-MS method was successfully applied in a pharmacokinetic study carried out in Sprague-Dawley rats after oral administration of aucubin at a single dose of 50mg/kg. Herein the pharmacokinetic study of aucubin is reported for the first time. © 2011 John Wiley & Sons, Ltd.

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