PubMed | Jinan Stomatologic Hospital, Navy General Hospital, The Military General Hospital of Beijing PLA and General Hospital of The Second Artillery
Type: Journal Article | Journal: Oncology letters | Year: 2015
Oral cavity cancer is common worldwide. Furthermore, the epidermal growth factor receptor (EGFR) signaling pathway is considered to be constitutively activated in oral cancers. Paclitaxel is widely accepted as an antitumor drug as it effectively inhibits the cell cycle. This study predominantly explores the possible molecule mechanism of paclitaxel on oral cancer treatment. Cell viability was first detected using an MTT assay. Cell apoptosis was examined by Hoechst staining and flow cytometry using an annexin-V and propidium iodide kit. Specific EGFR signaling pathways were further explored through western blot analysis. Abnormal protein expression levels were determined via immunofluoresence. Additionally, the protein levels of matrix metalloproteinase (MMP)-2 and 9 were determined using ELISA. Paclitaxel significantly inhibited oral cancer cell viability in a time- and dose-dependent manner. Paclitaxel also enhanced oral cancer cell apoptosis via increased Bim and Bid protein expression. Furthermore, paclitaxel was observed to inhibit oral cancer cell proliferation through increased MMP-2 and MMP-9 protein levels. Paclitaxel inhibited the growth of the oral cancer cell line, tea8113 malignant proliferation and enhanced tea8113 cell apoptosis through inhibiting the EGFR signaling pathway.
Kong L.,Jinan Stomatologic Hospital |
Kong L.,University of Sichuan |
Qi X.,Hebei Medical University |
Huang S.,University of Sichuan |
And 3 more authors.
Archives of Oral Biology | Year: 2015
Objectives Theaflavins, the main polyphenols in black tea, possesses a wide range of beneficial pharmacological properties. Porphyromonas gingivalis (P. gingivalis) is a major aetiological agent associated with periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues. The aim of the present study is to investigate the effect of theaflavins on pathogenic properties of P. gingivalis and on periodontitis by inhibiting matrix metalloproteinases (MMPs) production induced by this oral pathogen. Methods Microplate dilution assays were performed to determine the effect of theaflavins against planktonic culture and biofilm of P. gingivalis. The effect of theaflavins on gingipain and collagenase activities of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrate. Human gingival fibroblasts (HGFs) were stimulated with P. gingivalis in the presence or absence of theaflavins, and then MMP-1,-2 secretion and their mRNA expression were assessed using an enzyme-linked immunosorbent assay (ELISA) and real-time PCR analysis, respectively. Results Theaflavins exhibited the antimicrobial effects against both planktonic culture and biofilm of P. gingivalis. Theaflavins also markedly inhibited the proteinase activities of P. gingivalis collagenase and gingipains in a dose-dependent manner. Lastly, theaflavins significantly inhibited the secretion and mRNA expression of MMP-1 and MMP-2 by HGFs stimulated with P. gingivalis. Conclusion Theaflavins can affect the virulent properties of P. gingivalis and attenuate the MMP-mediated inflammatory response induced by this pathogen, which suggests that theaflavins may be potentially valuable supplementary therapeutic agent for prevention and treatment of P. gingivalis-associated periodontal diseases. © 2014 Elsevier Ltd. All rights reserved.
Cui J.,Jinan Stomatologic Hospital |
Cui J.,Shandong University |
Li D.,Jinan Stomatological Hospital |
Zhang W.,Jinan Stomatological Hospital |
And 2 more authors.
Oncology Letters | Year: 2014
MicroRNAs (miRNAs) are important in the regulation of cell growth, differentiation, apoptosis and carcinogenesis. The overexpression of oncogenic miRNAs or the underexpression of tumor suppressor miRNAs exhibits a critical function in the tumorigenesis of oral cancer. The aim of the present study was to identify differentially expressed miRNAs (DE-miRNAs), which may differentiate oral cancer from normal tissues, as well as the molecular signatures that differ in tumor histology. The miRNA expression profiles of GSE28100 [the Gene Expression Omnibus (GEO) accession number] were downloaded from the GEO database and an independent sample t-test was used to identify statistical differences between the DE-miRNAs of the oral cancer patients and the healthy control subjects. The target genes of DE-miRNA were retrieved from the miRecords database. Furthermore, a protein-protein interaction network was constructed using the Search Tools for the Retrieval of Interacting Genes database and Cytoscape software. A total of 15 DE-miRNAs were identified and among them, hsa-miR-15a drew specific attention. Gene Ontology analysis revealed that the target genes of fibroblast growth factor (FGF)2 are involved in the progression of oral cancer. Furthermore, functional analysis indicated that the FGF-receptor signaling pathway was significantly upregulated in oral cancer. hsa-miR-15a is important in the regulation of oral cancer and thus, may present a potential biomarker for the prediction of oral cancer progression.
Cui J.,Jinan Stomatologic Hospital |
Shen L.,Jinan Stomatologic Hospital |
Wang Y.,Shandong University
Asian Pacific Journal of Cancer Prevention | Year: 2012
Background: The Cyclin D1(CCND1) G870A polymorphism may be associated with breast cancer, but the evidence from individual studies is inconclusive. The aim of this study was to investigate the correlation between the CCND1 G870A polymorphism and breast cancer risk in a meta-analysis. Materials and Methods: We searched Pubmed and analysed 11 articles on 5,528 cases and 5,353 controls before February 1, 2012. Results: we found there are significant association for AA versus GG and AA versus GA/GG. No significant associations were found for GA versus GG, GA/AA versus GG. There are significant association for AA versus GG ,and AA versus GA/GG in Caucasians. We didn't find any significant main effects for G870Apolymorphism on breast cancer risk either in recessive or dominant models in Asians. Conclusion: This meta-analysis suggests that AA of the CCND1 G870A polymorphism is associated with breast cancer susceptibility.
Sun J.,Shandong University |
Liu X.,Jinan Stomatologic Hospital |
Jiang G.,Shandong University |
Qi Q.,Shandong University
Antimicrobial Agents and Chemotherapy | Year: 2015
Candida albicans persisters constitute a small subpopulation of biofilm cells and play a major role in recalcitrant chronic candidiasis; however, the mechanism underlying persister formation remains unclear. Persisters are often described as dormant, multidrug-tolerant, nongrowing cells. Persister cells are difficult to isolate and study not only due to their low levels in C. albicans biofilms but also due to their transient, reversible phenotype. In this study, we tried to induce persister formation by inducing C. albicans cells into a dormant state. C. albicans cells were pretreated with 5-fluorocytosine (planktonic cells, 0.8 μgml-1; biofilm cells, 1 μgml-1) for 6 h at 37°C, which inhibits nucleic acid and protein synthesis. Biofilms and planktonic cultures of eight C. albicans strains were surveyed for persisters after amphotericin B treatment (100 μgml-1 for 24 h) and CFU assay. None of the planktonic cultures, with or without 5-fluorocytosine pretreatment, contained persisters. Persister cells were found in biofilms of all tested C. albicans strains, representing approximately 0.01 to 1.93% of the total population. However, the persister levels were not significantly increased in C. albicans biofilms pretreated with 5-fluorocytosine. These results suggest that inhibition of nucleic acid synthesis did not seem to increase the formation of amphotericin B-tolerant persisters in C. albicans biofilms. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Wang B.,Hefei Stomatologic Hospital |
Huang S.,Shandong University |
Pan L.,Jinan Stomatologic Hospital |
Jia S.,Shandong University
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology | Year: 2013
Objectives: The aim of this study was to investigate if overexpression of osterix (Osx) in human umbilical cord-derived mesenchymal stem cells (UC-MSCs) would facilitate osteogenic differentiation in bone regeneration. Study Design: UC-MSCs were isolated from UCs. A pEGFP-Osx plasmid was constructed and applied to transfect UC-MSCs. Cell proliferation, alkaline phosphatase (ALP) activity, and expression of bone-related genes were examined to evaluate the osteogenic potential of UC-MSCs. Bone regeneration in vivo was evaluated in nude mice using PLGA as a carrier. Results: Reverse-transcription polymerase chain reaction showed that pEGFP-Osx transfection enhanced expression of bone matrix proteins. Overexpression of Osx in UC-MSCs enhanced ALP activity, while not inhibited their proliferation rate. The Osx-transduced group formed significantly more bone at 4 weeks. Conclusions: Concerning their simple isolation and proliferation, it is believed that genetically engineered UC-MSCs could play important roles in the study and application of bone tissue engineering. © 2013 Elsevier Inc.
Mi Y.,Jinan Stomatologic Hospital
Life Science Journal | Year: 2013
Objective This study aimed to evaluate the clinical performance of gold alloy inlays, porcelain inlays and Ceramage polymerization porcelain inlays in restoring molar defects. Materials and methods Gold alloy inlays, Ceramage polymerization porcelain inlays and porcelain inlays were used to restore 59, 54 and 51 molar defects, respectively. All restorations were evaluated 6, 18 and 30 months after restoration according to the modified standards proposed by the American Public Health Association. Results No significant differences in inlay discoloration, marginal adaptation, secondary caries and food impaction were observed among these groups (P > 0.05). Both gold alloy inlays and Ceramage inlays were significantly more resistant to fractures than porcelain inlays (P < 0.05). Conclusions Gold alloy inlays, Ceramage inlays and porcelain inlays all produced satisfactory restorative effects on molar defects, although some differences were observed. An appropriate method should be determined according to patient conditions.
Shen L.,Shandong University |
Cui J.,Jinan Stomatologic Hospital |
Liang S.,Shandong University |
Pang Y.,Shandong University |
Liu P.,Shandong University
OncoTargets and Therapy | Year: 2013
Accumulating evidence shows that enhancer of zeste homolog 2 (E2H2) is upregulated in a broad range of cancer types, such as breast cancer, prostate cancer, ovarian cancer, and colon cancer. Therefore, inhibiting EZH2 expression may be a promising strategy for anticancer therapy. This review focuses on the current understanding of the mechanisms underlying EZH2 regulation that are involved in cancer progression. Also, it introduces two EZH2 inhibitors that target EZH2 and could be potentially applied in the treatment of cancer in the future. © 2013 Shen et al, publisher and licensee Dove Medical Press Ltd.
Shen L.,Shandong University |
Cui J.,Jinan Stomatologic Hospital |
Pang Y.-X.,Shandong University |
Ma Y.-H.,Shandong University |
Liu P.-S.,Shandong University
Asian Pacific Journal of Cancer Prevention | Year: 2013
methyltransferase inhibitor, disrupts polycomb-repressive complex 2 (PRC2), and induces apoptosis, while inhibiting proliferation and metastasis, in cancer cells, including acute myeloid leukemia, breast cancer and glioblastoma. However, little is known about effects of DZNep on ovarian cancer cells. Materials and Methods: We here therefore studied DZNep-treated A2780 ovarian cancer cells in vitro. Proliferation of ovarian cancer cells under treatment of DZNep was assessed by MTT and apoptosis by flow cytometry. Cell wound healing was applied to detect the migration. Finally, we used q-PCR to assess the migration-related gene, E-cadherin. Results: DZNep could inhibit the proliferation of A2780 and induce apoptosis Furthermore, it inhibited migration and increased the expression of E-cadherin (P<0.05). Conclusion: DZNep is a promising therapeutic agent for ovarian cancer cells, with potential to inhibite proliferation, induce apoptosis and decrease migration.
PubMed | Jinan Stomatologic Hospital and Shandong University
Type: Journal Article | Journal: PloS one | Year: 2014
Ectopic angiogenesis within the intima and media is considered to be a hallmark of advanced vulnerable atherosclerotic lesions. Some studies have shown that specific matrix metalloproteinases (MMPs) might play different roles in angiogenesis. Therefore, we investigated the predominant effects of specific MMPs in intraplaque angiogenesis and plaque instability in a rabbit model of atherosclerosis.New Zealand rabbits underwent balloon injury of the abdominal artery and ingestion of a high-cholesterol (1%) diet to establish an atherosclerotic animal model. At weeks 4, 6, 8, 10, and 12 after balloon injury, five rabbits were euthanized and the abdominal aorta was harvested. Blood lipid analysis, intravascular ultrasound imaging, pathologic and immunohistochemical expression studies, and western blotting were performed. From weeks 4 to 12, the expression of MMP-1, -2, -3, and -9 and vascular endothelial growth factor A (VEGF-A) increased with atherosclerotic plaque development in the abdominal aorta, while the expression of MMP-14 substantially decreased. The vulnerability index (VI) gradually increased over time. Intraplaque neovessels appeared at week 8. The microvessel density (MVD) was greater at week 12 than at week 8. The VI, MVD, and VEGF-A level were positively correlated with the MMP-1, -2,-3, and -9 levels within plaques. Negative correlations were noted between the MMP-14 level and the VI, MVD, and VEGF-A level.Upregulation of MMP-1, -2, -3, and -9 and downregulation of MMP-14 may contribute to intraplaque angiogenesis and plaque instability at the advanced stage of atherosclerosis in rabbits.