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Yu H.,Shandong Cancer Hospital and Institute | Zhang L.,Jinan Fourth Peoples Hospital | Du X.,Shandong Cancer Hospital and Institute | Sheng X.,Shandong Cancer Hospital and Institute
Chinese Journal of Clinical Oncology | Year: 2014

Objective: The benefits of postoperative adjuvant therapy method for low-risk early-stage cervical squamous cell carcinoma were investigated. Methods: A total of 133 patients with low-risk early-stage cervical squamous cell carcinoma were treated at Shandong Cancer Hospital & Institute from February 2008 to March 2012. All patients received adjuvant therapy: 42 were treated with pelvic radiotherapy (RT), 47 were treated with adjuvant chemotherapy (CT) + intracavitary radiotherapy (ICRT), and 44 were treated with concurrent chemoradiation (CCRT). Disease-free survival (DFS) and complications of the therapy were evaluated. Results: No significant differences in DFS were observed in the patients treated with RT, CT + ICRT, and CCRT (P>0.05), and the three-year DFS rates were 94.0%, 93.4%, and 97.6%, respectively. The frequencies of grade III to IV acute toxicities were significantly higher in patients treated with CCRT (34.1%) than in those treated with RT (9.5%) or CT + ICRT (16.7%) (P<0.05). No statistically significant difference was observed between the RT group and the CT + ICRT group (P>0.05). Grade I to II late toxicity was significantly more frequent in the CCRT (25%) and RT (19.0%) groups compared with the CT + ICRT group (4.3%) (P>0.05), but no statistically significant differences were observed between the CCRT and the RT groups (P>0.05). Conclusion: CT + ICRT or RT has a three-year DFS rate equivalent to CCRT but with fewer therapy complications for low-risk early-stage cervical squamous cell carcinoma. Source

Zhao Y.,Affiliated Hospital of Academy of Military Medical science | Zhao Y.,Chinese Institute of Microbiology and Epidemiology | Zhao Y.,Liaoning Medical University | Niu W.,Affiliated Hospital of Academy of Military Medical science | And 9 more authors.
BioMed Research International | Year: 2015

An S. maltophilia strain named WJ66 was isolated from a patient; WJ66 showed resistance to more antibiotics than the other S. maltophilia strains. This bacteraemia is resistant to sulphonamides, or fluoroquinolones, while the representative strain of S. maltophilia, K279a, is sensitive to both. To explore drug resistance determinants of this strain, the draft genome sequence of WJ66 was determined and compared to other S. maltophilia sequences. Genome sequencing and genome-wide evolutionary analysis revealed that WJ66 was highly homologous with the strain K279a, but strain WJ66 contained additional antibiotic resistance genes. Further analysis confirmed that strain WJ66 contained an amino acid substitution (Q83L) in fluoroquinolone target GyrA and carried a class 1 integron, with an aadA2 gene in the resistance gene cassette. Homology analysis from the pathogen-host interaction database showed that strain WJ66 lacks raxST and raxA, which is consistent with K279a. Comparative genomic analyses revealed that subtle nucleotide differences contribute to various significant phenotypes in close genetic relationship strains. © 2015 Yan Zhao et al. Source

Yang H.,Shandong University | Lu X.,Shandong University | Liu Z.,Jinan Fifth Peoples Hospital | Chen L.,Jinan Fourth Peoples Hospital | And 4 more authors.
Oncotarget | Year: 2015

Epithelial-mesenchymal transition (EMT) plays a fundamental role in cancer metastasis. The ubiquitin ligase FBXW7, a general tumor suppressor in human cancer, has been implicated in diverse cellular processes, however, its role in cholangiocarcinoma (CCA) metastasis has not been identified. Here, we report a crucial role of FBXW7 in CCA metastasis by regulating EMT. Loss of FBXW7 expression was detected in CCA cells and clinical specimens. Clinicopathological analysis revealed a close correlation between FBXW7 deficiency and metastasis, TNM stage and differentiation in intrahepatic CCA and perihilar CCA. Moreover, FBXW7 silencing in CCA cells dramatically promoted EMT, stem-like capacity and metastasis both in vitro and in vivo. Conversely, FBXW7 overexpression attenuated these processes. Mechanistically, treatment with rapamycin, a mTOR inhibitor, inhibited EMT, stemlike capacity and metastasis induced by FBXW7 silencing both in vitro and in vivo. Furthermore, the expression of EMT regulating transcription factors, snail, slug and ZEB1, were also decreased markedly with rapamycin treatment. In addition, silencing ZEB1 inhibited EMT and metastasis of both CCA cells and FBXW7 deficient CCA cells, which implicated the potential role of ZEB1 in FBXW7/mTOR signaling pathway related CCA metastasis. In conclusion, our findings defined a pivotal function of FBXW7 in CCA metastasis by regulating EMT. Source

Yang C.H.,Jinan Fourth Peoples Hospital
Advanced Materials Research | Year: 2014

Polyembryony is an infrequent situation in the conceived naturally. Pregnant woman would have some changes about several physical signs during pregnancy period. Some women even have some certain pregnancy reaction. For the pregnant women with multiple births, they would feel more discomfort during pregnancy period, and they have more probability to generate physical signs, polyembryony ups and downs increases accordingly. Not only during the pregnancy period, but also in the farrowing interval and after parturition the pregnant women with multiple births have much more probability to arise problems. Thus, for the nursing of multiple pregnancy complication, we should have foreseeability and prevention. This paper combines with common clinical data, and then discusses multiple pregnancy complication and treatment and nursing. © (2014) Trans Tech Publications, Switzerland. Source

Li H.,Jinan Fourth Peoples Hospital | Yang X.,Shandong Qilu Hospital | Shi W.,Shandong University | Ma Z.,Jinan Fourth Peoples Hospital | And 4 more authors.
Biomedical and Environmental Sciences | Year: 2015

Recent studies showed that pathology of alcoholic encephalopathy was associated with cerebral vascular damage. TMP (tetramethyl-pyrazine) is widely used in the treatment of cerebrovascular diseases, however, it has not been reported whether TMP can relieve alcohol-induced cerebral vascular damages. The study was performed to investigate the learning and memory, cerebrovascular pathological changes and the expressions of vascular endothelial growth factor (VEGF) and serum levelsofendothelin-1 (ET-1) in the rat model of chronic alcoholic encephalopathy, and explore the effects of TMP intervention on alcoholic encephalopathy. In the present study, the rat model of chronic alcoholic encephalopathy was established by the gavage administration of alcohol; the learning and memory ability was tested by Morris water maze; the expression of VEGF was measured by RT-PCR and Western blot; and the serum levels of ET-1 was measured by radioimmunoassay. We found that alcohol intoxication impaired learning and memory, induced VEGF overexpression and increased ET 1 concentrations. TMP intervention improved learning abilities, increased the VEGF expression and reduced ET-1 level. These results indicate that TMP exhibits therapeutic effects on chronic alcoholic encephalopathy. © 2015 The Editorial Board of Biomedical and Environmental Sciences. Source

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