Gao J.,the 401st Hospital of Jinan Military Area |
Wang M.,Jinan Central Hospital Jinan 250013 |
Yang S.,Jinan Central Hospital Jinan 250013 |
Li X.,Weifang Medical University
Shanghai kou qiang yi xue = Shanghai journal of stomatology | Year: 2015
PURPOSE: To analyze the effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells and to investigate the mechanism of migration.METHODS: The inguinal adipose tissue of rat was excised at aseptic condition to obtain primary ADSCs by enzyme digestion. Multi-directional differentiation was used to identify the ADSCs. PRF membrane was acquired through one time centrifuge. The cell migration was examined by Transwell assay and wound healing assay. The mRNA expression of MMP2 and MT1-MMP was tested by real-time PCR. Statistical analysis was performed using SPSS 13.0 software package.RESULTS: Cell migration test showed that the migration of rat ADSCs in PRF group were significantly higher than those in the negative group（P<0.05） and inhibitor group（P<0.05）. The ADSCs migration effects in three growth factors group at different concentrations showed significant difference（P<0.05）. Real-time PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in PRF group than control group (P<0.05). PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in three growth factors group than control group (P<0.05).CONCLUTIONS: PRF and three growth factors consistently enhanced the migration of rat ADSCs in a dose-response manner. The migration increase of rat ADSCs may be associated with the up-regulation of MMP2 and MT1-MMP gene expression.
PubMed | General Hospital of Jinan Military Area Jinan 250031, The Fourth Hospital of Jinan Jinan 250013 and Jinan Central Hospital Jinan 250013
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2016
To do a systematic review using meta-analysis to assess the diagnostic accuracy of fecal lactoferrin (FL) in patients with inflammatory bowel disease (IBD).We performed a literature review and systematically searched the Medline and EMBASE databases for eligible studies. The quality of the included studies was assessed using the QUADAS tool. The sensitivity, specificity, and other diagnostic indexes of FL were pooled using a random-effects model.Seven studies, involving 1816 patients, met the inclusion criteria. In all studies, the pooled FL sensitivity and pooled specificity were 0.82 (95% confidence interval [CI]: 0.72, 0.89) and 0.95 (95% CI: 0.88, 0.98), respectively. The positive and negative likelihood ratios were 16.63 and 0.18, respectively. The area under the summary receiver-operating characteristic curve (SROC) was 0.95 (95% CI: 0.93, 0.97), and the diagnostic odds ratio was 90.04 (95% CI: 37.01, 219.02). The pooled FL sensitivity and specificity for Crohns disease (CD) diagnosis (sensitivity =75%, specificity =100%) was not as good as it was for ulcerative colitis (UC) diagnosis (sensitivity =82%, specificity =100%).FL, as a noninvasive and screening marker, has a high specificity and a modest specificity during the diagnosis of suspected IBD.